CN114762498A - Biological material processing apparatus - Google Patents
Biological material processing apparatus Download PDFInfo
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- CN114762498A CN114762498A CN202110055001.6A CN202110055001A CN114762498A CN 114762498 A CN114762498 A CN 114762498A CN 202110055001 A CN202110055001 A CN 202110055001A CN 114762498 A CN114762498 A CN 114762498A
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- liquid
- cabin
- shell
- liquid exchange
- discharge
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
- A01N1/0268—Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen
Abstract
The invention belongs to the technical field of biological material treatment and discloses a biological material treatment device. The liquid exchange device comprises a shell, wherein the upper end of the shell is provided with a liquid exchange cabin, a liquid discharge cabin and a plurality of liquid guide channels, and two ends of each liquid guide channel are respectively communicated with the liquid exchange cabin and the liquid discharge cabin so as to realize liquid exchange between the liquid exchange cabin and the liquid discharge cabin; the lower end of the liquid exchange cabin is communicated with a discharge hole, and the discharge hole is communicated with the lower end of the shell. The invention provides enough space in the shell to form an effect similar to a culture dish, facilitates the exchange of treatment liquid in a container of biological cells or tissues, controls the volume of liquid around the biological material within a preset range, ensures the stability and safety of operation, ensures that the operation process is more convenient and efficient, can develop various different applications according to different use occasions, and is convenient for transferring the biological material to other specific positions and devices subsequently.
Description
Technical Field
The invention belongs to the technical field of biological material treatment, and particularly relates to a biological material treatment device.
Background
In laboratories, hospitals and the like, it is often necessary to perform solution treatment on biological materials, including biological cells, biological tissues and the like, in order to exchange liquid inside the cells with the outside, so as to perform subsequent experimental purposes.
The art of vitrification cryopreservation of human and animal embryos is currently a relatively mature art, where "freezing" is liquid to solid cooling, which may include crystallization, and "vitrification" is liquid to solid cooling, but not crystallization. The vitrifying cryopreservation of human and animal embryos consists in the steps of collection and retrieval of the oocytes, their in vitro fertilization and subsequent storage of such fertilized eggs and the embryos and/or the resulting later blastocysts in an ultra-low temperature environment after treatment in a cryoprotective solution.
There are also many brand cryoprotectants on the market, with principles that are largely the same or different, in order to suppress the formation of ice crystals within cells and minimize cell damage during the freezing process. These cryoprotectants are classified as osmotic and non-osmotic solutions. Examples of permeability are Ethylene Glycol (EG), dimethyl sulfoxide (DMSO), and glycerol. The osmotic cryoprotectant is a small molecule that readily penetrates the biofilm, forming hydrogen bonds with water molecules of the biomaterial, preventing its ice crystallization. Impermeable cryoprotectants, such as disaccharides, trehalose, and sucrose, function by extracting free water from within the biological material and dehydrating the intracellular space. The resulting dehydration allows it to complement osmotic cryoprotectants to increase the intracellular relative concentration of cryoprotectants and, secondly, to prevent the formation of ice crystals within the cell. However, the toxicity of these high concentrations of cryoprotectants can be considerable and the cells need to be rapidly plunged into liquid nitrogen after cryoprotectant pretreatment to achieve freezing.
The vitrification process involves exposing the biological material to at least three vitrification solutions. The vitrification solution is typically added to successive wells of a multi-well culture dish, wherein the culture dish and solution are warmed to a predetermined temperature, which is determined according to the requirements of the biological material under investigation.
In a typical protocol, the biological material is physically transferred to a first solution (e.g., ES equilibrator) in a first well, and then washed by physically moving the biological material or cells through the solution of interest using a cell pipetting device. The washing process is repeated in a second solution (e.g., VS vitrification cryofluid) in a second well, in a third and fourth well for a predetermined period of time until the biological material or cells are considered ready for cryopreservation. The biological material is then physically aspirated with a predetermined amount of vitrification solution using a pipette or other manipulation device. The droplets containing the biological material or cells to be vitrified are then pipetted onto a vitrification device, such as a carrier rod. The vitrification device with the attached droplets and biological material is then physically transferred and directly plunged into liquid nitrogen. Once the biological material and carrier fluid are vitrified, the vitrification device is inserted into a pre-cooling protective sleeve or other storage device for subsequent transfer to liquid nitrogen or liquid nitrogen vapor for long-term cold storage.
The existing cell freezing process is basically completed manually. When people manually work, problems of mental stress, hand trembling, visual illusion, fatigue and the like can occur, and operation errors such as embryo or cell loss can occur, so that the stability, timeliness, safety and the like of operation cannot be guaranteed.
Disclosure of Invention
In order to solve the above problems of the prior art, the present invention is directed to a biomaterial treating apparatus.
The technical scheme adopted by the invention is as follows:
a biological material processing device comprises a shell, wherein a liquid exchange cabin, a liquid discharge cabin and a plurality of liquid guide channels are arranged at the upper end of the shell, and two ends of each liquid guide channel are respectively communicated with the liquid exchange cabin and the liquid discharge cabin and are used for realizing liquid exchange between the liquid exchange cabin and the liquid discharge cabin; the lower end of the liquid exchange cabin is communicated with a discharge hole, and the discharge hole is communicated with the lower end of the shell.
Further preferably, the liquid guide channel is provided at an upper end of the housing.
It is further preferred that the upper end of the housing is provided with a circular groove, and the liquid exchange compartment, the liquid discharge compartment and all the liquid guide channels are located in the circular groove.
Still more preferably, a cylindrical sleeve is arranged above the circular groove in a matching manner, and a piston rod is connected in the cylindrical sleeve in a sliding manner; and a thin rod for plugging is arranged in the discharge hole and is connected with one end of the piston rod close to the circular groove.
It is further preferred that the liquid exchange chamber and the liquid discharge chamber have respective diameters which become smaller from top to bottom.
Still more preferably, a load bar is provided at a lower end of the discharge hole.
It is further preferable that the housing is provided with a plurality of capillary chutes, and a lower end of each of the capillary chutes communicates with a lower end of the discharge hole.
It is further preferable that the lower part of the housing is horizontally provided with a limiting slot hole, and one end of the carrier rod is positioned at the lower end of the discharge hole after passing through the limiting slot hole.
More preferably, the cross section of the liquid exchange cabin is a semicircular cross section, an inner wall surface of the liquid exchange cabin is parallel to one side surface of the shell, and the shell is made of transparent materials; the liquid exchange chamber, the drain hole and the capillary chute are all located within the same depth of field.
It is further preferred that the housing has a notch in the middle of its lower end.
The invention has the beneficial effects that:
the invention is provided with a liquid exchange cabin, which can inject biological material and biological culture solution to be processed into the liquid exchange cabin to make the biological material exchange liquid in the culture solution, because the liquid exchange cabin is communicated with a liquid discharge cabin through a liquid guide channel, the solution in the liquid exchange cabin can be guided into the liquid discharge cabin through capillary action, then the excess solution is sucked away by a capillary tube or a chute dropper with a capillary action slit at the liquid discharge cabin, so that the solution in the liquid exchange cabin can be continuously guided into the liquid discharge cabin until the liquid exchange between the biological material and the solution is finished, and finally the biological material in the liquid exchange cabin enters the discharge hole along with the solution and is smoothly discharged, the invention forms an effect similar to a culture dish by providing enough space in a shell, which is convenient for the exchange of processing liquid in a container of biological cells or tissues, control the liquid volume around the biomaterial and predetermine the within range, combine the design of discharge hole, form the effect that is similar to the pipettor, guaranteed the stability and the security of operation for operation process is convenient more and high-efficient, and can develop multiple different applications according to the use occasion difference, the follow-up biomaterial of being convenient for simultaneously shifts other specific position and device to.
Drawings
Fig. 1 is a schematic perspective view of a first embodiment of the present invention;
FIG. 2 is a cross-sectional view of a first embodiment of the present invention;
FIG. 3 is a schematic perspective view of a second embodiment of the present invention;
FIG. 4 is a cross-sectional view of a second embodiment of the present invention;
FIG. 5 is a graph showing the effect of microscopic observation of the upper part in the experiment;
FIG. 6 is a graph showing the effect of the side microscopic observation in the experiment;
fig. 7 is a graph showing the effect of microscopic observation of the bottom in the experiment.
In the figure: 1-a shell; 2-liquid exchange chamber; 3-liquid discharge compartment; 4-a drainage channel; 5-a drain hole; 6-a circular groove; 7-capillary chutes; 8-limiting slotted holes; 9-carrying rod.
Detailed Description
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the present invention will be briefly described below with reference to the accompanying drawings and the embodiments or the description in the prior art, it is obvious that the following description of the structure of the drawings is only some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
The technical solution provided by the present invention will be described in detail by way of embodiments with reference to the accompanying drawings. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto.
In some instances, some embodiments are not described or not in detail, as they are conventional or customary in the art.
Furthermore, the technical features described herein, or the steps of all methods or processes disclosed, may be combined in any suitable manner in one or more embodiments, in addition to the mutually exclusive features and/or steps. It will be readily appreciated by those of skill in the art that the order of the steps or operations of the methods associated with the embodiments provided herein may be varied. Any sequence in the figures and examples is for illustrative purposes only and does not imply a requirement in a certain order unless explicitly stated to require a certain order.
The ordinal numbers used herein for the components, such as "first," "second," etc., are used merely to distinguish between the objects described, and do not have any sequential or technical meaning. The terms "connected" and "coupled" when used in this application, encompass both direct and indirect connections (and couplings) where appropriate and where not necessary contradictory.
The first embodiment is as follows:
as shown in fig. 1 and fig. 2, the present embodiment provides a biomaterial treatment apparatus, which includes a casing 1, a liquid exchange chamber 2 for exchanging a biomaterial liquid, a liquid discharge chamber 3 for discharging the liquid, and a plurality of liquid guide channels 4 are arranged at an upper end of the casing 1, and two ends of each liquid guide channel 4 are respectively communicated with the liquid exchange chamber 2 and the liquid discharge chamber 3 for realizing liquid exchange between the liquid exchange chamber 2 and the liquid discharge chamber 3; the lower end of the liquid exchange cabin 2 is communicated with a discharge hole 5 for discharging the biological materials after treatment, and the discharge hole 5 is communicated with the lower end of the shell 1. It should be noted that, when there is no specific limitation on the structures of the liquid exchange chamber 2, the liquid discharge chamber 3 and the liquid guide channel 4, the sizes and shapes thereof are not specifically limited, and can be adjusted according to actual situations.
It should be further described in this embodiment that the liquid guiding channel 4 is disposed at the upper end of the housing 1, so that the control of the solution is more accurate, and it should be described that the liquid guiding channel 4 is disposed at the upper end of the housing 1 only in an optimal manner, and can be adjusted according to the volume of the actual liquid exchange chamber 2 and the actual demand, and in the implementation process, the liquid guiding channel 4 may also be disposed inside the housing 1 without specific limitation.
It should be further noted in this embodiment that the upper end of the housing 1 is provided with a circular groove 6 for the requirements of the treatment process, and the liquid exchange chamber 2, the liquid discharge chamber 3 and all the liquid guide channels 4 are located in the circular groove 6, so as to facilitate the piston operation at the later stage. More specifically, a cylindrical sleeve is arranged above the circular groove 6 in a matching manner, and a piston rod is connected in the cylindrical sleeve in a sliding manner; a thin rod for plugging is arranged in the discharge hole 5, the thin rod is connected with one end of the piston rod close to the circular groove 6, and the thin rod can be a quartz capillary. It should be noted that the piston rod and the cylindrical sleeve can slide relatively, so the cylindrical sleeve can be buckled in the circular groove 6 according to the situation, or keep a certain distance from the circular groove 6, and the operation is convenient for the liquid exchange chamber 2 and the liquid discharge chamber 3.
It should be further noted that, in this embodiment, a notch is formed in the middle of the lower end of the casing 1, so as to facilitate taking the casing 1, and meanwhile, a certain operation space is also left below the discharge hole 5, so that the casing 1 is more like a platform, and the solution discharged from the discharge hole 5 is associated with the biological material, so as to facilitate subsequent operations.
Initially, the position of the discharge hole 5 is closed by a thin rod having a corresponding size or a medical catheter, and leakage of the liquid is prevented. In order to achieve a better sealing effect, it is conceivable to apply a substance such as medical paraffin to the passage of the discharge hole 5, and to facilitate the movement of the pin in the hole. Biological material biological cells or biological tissues to be treated are injected into the liquid exchange chamber 2 together with a certain amount of culture solution, and since the discharge holes 5 are blocked by the thin rods, it can be ensured that the biological material is not lost in the liquid exchange chamber 2. The liquid exchange cabin 2 is continuously filled with a specific biological culture liquid, so that the biological materials are subjected to liquid exchange in the culture liquid. In the process, as the liquid guide channel 4 is communicated between the liquid exchange cabin 2 and the liquid discharge cabin 3, the size of the liquid guide channel 4 is specially designed, the principle that the solution in the liquid exchange cabin 2 is guided into the liquid discharge cabin 3 through capillary action and the liquid is sucked at the position is similar to the structure of an open channel in the prior art, and then the redundant solution is sucked away by a dropper at the position of the liquid discharge cabin 3, so that the solution in the liquid exchange cabin 2 can be continuously guided into the liquid discharge cabin 3. This procedure is repeated with different culture fluids until the fluid exchange between the biological material and the solution is completed and the remaining solution is optimally drained by the drainage channel 4, and a certain amount of solution and processed biological material remains inside the fluid exchange chamber 2. At this moment, the whole circular groove 6 can be sleeved by a cylindrical sleeve above the device, the sleeve opening of the cylindrical sleeve can be clamped at the circular groove 6 for sealing, and the cylindrical sleeve is internally provided with a piston rod to form a piston structure similar to a syringe with the sleeve. The piston rod is connected with the thin rod, when the piston rod is upwards, the thin rod can be pulled out of the discharge hole 5, and the discharge hole 5 is exposed to form a needle head structure communicated with the outside. At this time, since the inside of the cylindrical casing is under negative pressure, the solution and the biomaterial do not leak from the discharge hole 5. Then the piston rod is pressed down, the increased pressure can drive the biological material to enter the discharge hole 5 along with the solution and smoothly discharge the biological material, and before the process is carried out, a part of solution can be extracted by utilizing the capillary tube, so that the volume control of the solution is realized. Below the outlet opening 5, a device for receiving the biological material, for example a carrier rod or a petri dish, can be placed, and the solution to be treated, together with the biological material, can be transferred to the desired device for subsequent treatment after it has been discharged through the outlet opening 5. The process realizes the treatment of the biological material in the container, the control of the volume of the solution after the treatment and the subsequent transfer of the biological material to other specific positions and devices.
The second embodiment:
as shown in fig. 3 and fig. 4, the present embodiment provides a biological material processing apparatus, which includes a housing 1, a liquid exchange chamber 2 for liquid exchange of biological material, a liquid discharge chamber 3 for liquid discharge, and a plurality of liquid guide channels 4 are arranged at an upper end of the housing 1, and two ends of each liquid guide channel 4 are respectively communicated with the liquid exchange chamber 2 and the liquid discharge chamber 3 for liquid exchange between the liquid exchange chamber 2 and the liquid discharge chamber 3; the lower end of the liquid exchange cabin 2 is communicated with a discharge hole 5 for discharging the biological tissue after treatment, and the discharge hole 5 is communicated with the lower end of the shell 1.
As a preferred mode, it needs to be further described in this embodiment that the liquid guide channel 4 is disposed at the upper end of the housing 1, so that the control of the solution is more accurate, it needs to be described that the liquid guide channel 4 is disposed at the upper end of the housing 1 only in a preferred mode, and the liquid guide channel 4 may be adjusted according to the volume of the actual liquid exchange cabin 2 and the actual requirement, and in the implementation process, the liquid guide channel 4 may also be disposed inside the housing 1, which is not limited specifically.
In this embodiment, it should be further noted that the lower end of the discharge hole 5 is provided with a carrying rod 9 for discharging the processed embryo together with the residual liquid downward to the carrying rod 9. The shell 1 is provided with a plurality of capillary chutes 7, the lower end of each capillary chute 7 is communicated with the lower end of the discharge hole 5, so that the redundant liquid on the carrying rod 9 is automatically sucked through the capillary action, the waste liquid around the discharged embryo is ensured to be avoided, and the structure of the capillary chute 7 is not particularly limited, such as a round hole, a polygonal hole or an annular hole. The lower part of the shell 1 is horizontally provided with a limit slot hole 8, and one end of the carrying rod 9 passes through the limit slot hole 8 and then is positioned at the lower end of the discharge hole 5, and can be close to the discharge hole 5, or can be attached to the discharge hole 5, or is an optimal distance (according to actual conditions) for facilitating the transfer of liquid drops to the rod. It should be noted that the length, width and shape of the groove body of the capillary chute can be made higher according to the requirement. Besides, besides the chute structure, the chute structure can be replaced by a vertical groove body, or a capillary tube is embedded in the vertical cylindrical groove body, or a ring of capillary tubes surrounding the discharge hole 5, so that a similar liquid sucking function is realized.
It should be further described in this embodiment that, a notch is formed in the middle of the lower end of the casing 1, so that the casing 1 can be taken conveniently, a certain operation space can be reserved below the discharge hole 5, the casing 1 is more like a platform which is erected, so that the solution discharged from the discharge hole 5 is more convenient for subsequent operations together with the biological material, and meanwhile, a certain space can be provided for the capillary chute 7, so that the capillary chute 7 can be a through hole structure without penetrating through the upper end of the casing 1, and the solution can be prevented from accidentally falling into the capillary chute 7 in the operation process, and the capillary chute 7 is prevented from exerting an effect.
In the operation, the discharge hole 4 is first closed with a narrow tube, and the embryo and a predetermined amount of culture solution are injected into the liquid exchange chamber 2. Then, a proper amount of biological treatment fluid ES is dripped into the liquid discharge cabin 3, and after the liquid enters the liquid exchange cabin 2 through the liquid guide channel 4, the liquid exchanges components with the embryo. After a sufficient treatment time, the liquid in the discharge chamber 3 is again withdrawn through the capillary micro-slits. The excess waste liquid in the liquid exchange chamber 2 is also sucked up through the liquid guide channel 4. Because the embryo will sit at the bottom of the ES solution during processing, the embryo will not be drawn away by the capillary tube under the restriction of the drainage channel 4. After the ES treatment is finished, VS is dripped into the liquid discharge cabin 3 in the same way, so that the VS enters the liquid exchange cabin 2 to exchange components with the embryo. After a sufficient processing time, the exchange between the embryo and the culture medium is completed, whereupon the thin tube blocking the discharge hole 5 is withdrawn and pressurized over the entire device, so that the embryo and the culture medium are driven under pressure into the hole 4 and therewith fall onto the carrier rod 9. Initially, a large amount of liquid is left around the embryo on the carrying rod 9, and under the action of the capillary chutes 7 on the two sides, the liquid is gradually sucked away, and a small amount of remaining liquid remains around the embryo. The carrier rod 9 with the embryos thereon may now be subjected to a subsequent vitrification freezing operation.
As a preferable mode, it should be further explained in this embodiment that the caliber of the liquid exchange chamber 2 and the caliber of the liquid discharge chamber 3 are gradually reduced from top to bottom, so that the capillary tube can more sufficiently suck the liquid at the liquid discharge chamber 3.
It should be noted that, the width, depth, shape, etc. of the liquid guiding channel 4 can be replaced according to the requirement, so as to meet the application requirement under the condition of different liquid amounts.
As a preferable mode, it should be further explained in this embodiment that the cross section of the liquid exchange chamber 2 is a semicircular cross section, an inner wall surface of the liquid exchange chamber 2 is parallel to a side surface of the housing 1, and the housing 1 is made of a transparent material, so that the position of the embryo in the liquid exchange chamber 2 can be conveniently observed through the position of the flat edge, and the reliability of the operation is ensured; the liquid exchange chamber 2, the drain hole 5 and the capillary chute 7 are all located in the same depth of field range, and can be observed from the upper part, the bottom or the side surface in the treatment process, so that the internal conditions of the three can be conveniently observed at any time under a microscope, as shown in fig. 5-7, wherein arrows in the three figures point to polystyrene micro-particles with the size of 200 microns, and represent biological materials.
It should be noted that the diameter and depth of the discharge holes 5 can be modified according to the size of the biological material to be treated, ensuring the device is suitable for different uses.
It should be noted that the carrier bar 9 used in this embodiment is preferably a cryotop model, and for other models of carrier bars, the bottom interface can be changed to interface with it. Cryotop is an elastic strip of plastic joined to a grip. Wherein the cells were placed on a strip and then directly plunged into liquid nitrogen. At present, Cryotop has a high survival rate and relative easy operability, and becomes a mainstream technology.
The present invention is not limited to the above-mentioned alternative embodiments, and any other various products can be obtained by anyone in the light of the present invention, but any changes in the shape or structure thereof, all of which fall within the scope of the present invention, fall within the protection scope of the present invention.
Claims (10)
1. A biomaterial treatment device, characterized in that: the device comprises a shell (1), wherein a liquid exchange cabin (2), a liquid discharge cabin (3) and a plurality of liquid guide channels (4) are arranged at the upper end of the shell (1), and two ends of each liquid guide channel (4) are respectively communicated with the liquid exchange cabin (2) and the liquid discharge cabin (3) so as to realize liquid exchange between the liquid exchange cabin (2) and the liquid discharge cabin (3); the lower end of the liquid exchange cabin (2) is communicated with a discharge hole (5), and the discharge hole (5) is communicated with the lower end of the shell (1).
2. A biological material processing apparatus according to claim 1, wherein: the liquid guide channel (4) is arranged at the upper end of the shell (1).
3. A biological material processing apparatus according to claim 1, wherein: the upper end of the shell (1) is provided with a circular groove (6), and the liquid exchange cabin (2), the liquid discharge cabin (3) and all the liquid guide channels (4) are all positioned in the circular groove (6).
4. A biomaterial treatment device as claimed in claim 3, wherein: a cylindrical sleeve is arranged above the circular groove (6) in a matched mode, and a piston rod is connected in the cylindrical sleeve in a sliding mode; and a thin rod for plugging is arranged in the discharge hole (5), and the thin rod is connected with one end of the piston rod close to the circular groove (6).
5. A biomaterial treatment device as claimed in claim 1, wherein: the caliber of the liquid exchange cabin (2) and the caliber of the liquid discharge cabin (3) are gradually reduced from top to bottom.
6. A biomaterial treatment device according to any one of claims 1 to 5, wherein: the lower end of the discharge hole (5) is provided with a load bar (9).
7. A biomaterial treatment device as claimed in claim 6, wherein: the shell (1) is provided with a plurality of capillary chutes (7), and the lower end of each capillary chute (7) is communicated with the lower end of the discharge hole (5).
8. A biomaterial treatment device as claimed in claim 6, wherein: the lower part of the shell (1) is horizontally provided with a limiting slotted hole (8), and one end of the carrying rod (9) passes through the limiting slotted hole (8) and then is positioned at the lower end of the discharge hole (5).
9. A biomaterial treatment device as claimed in claim 6, wherein: the cross section of the liquid exchange cabin (2) is a semicircular cross section, one inner wall surface of the liquid exchange cabin (2) is parallel to one side surface of the shell (1), and the shell (1) is made of transparent materials; the liquid exchange cabin (2), the discharge hole (5) and the capillary chute (7) are all positioned in the same depth of field range.
10. A biomaterial treatment device as claimed in claim 6, wherein: the middle part of the lower end of the shell (1) is provided with a notch.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115885973A (en) * | 2022-11-21 | 2023-04-04 | 香港中文大学深港创新研究院(福田) | Vitrification freezing tool and using method thereof |
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| CN103179852A (en) * | 2010-05-28 | 2013-06-26 | 格尼亚有限公司 | Improved micromanipulation and storage apparatus and methods |
| CN110521719A (en) * | 2019-07-08 | 2019-12-03 | 浙江大学 | A kind of glass freezing device and application method |
| CN111700064A (en) * | 2020-07-22 | 2020-09-25 | 明日加加科技有限公司 | Cell freezing auxiliary device |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103179852A (en) * | 2010-05-28 | 2013-06-26 | 格尼亚有限公司 | Improved micromanipulation and storage apparatus and methods |
| CN110521719A (en) * | 2019-07-08 | 2019-12-03 | 浙江大学 | A kind of glass freezing device and application method |
| CN111700064A (en) * | 2020-07-22 | 2020-09-25 | 明日加加科技有限公司 | Cell freezing auxiliary device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115885973A (en) * | 2022-11-21 | 2023-04-04 | 香港中文大学深港创新研究院(福田) | Vitrification freezing tool and using method thereof |
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