CN114748407B

CN114748407B - Traditional Chinese medicine mask for whitening and acne removal

Info

Publication number: CN114748407B
Application number: CN202210520797.2A
Authority: CN
Inventors: 金丽华; 胡德新
Original assignee: Second Affiliated Hospital of ZCMU
Current assignee: Second Affiliated Hospital of ZCMU
Priority date: 2022-05-12
Filing date: 2022-05-12
Publication date: 2023-05-16
Anticipated expiration: 2042-05-12
Also published as: CN114748407A

Abstract

The invention relates to a traditional Chinese medicine mask for whitening and acne removal, which is prepared from radix angelicae, rose, semen cuscutae, concha haliotidis, poria cocos, coix seed, rhizoma typhonii, bletilla striata, radix ampelopsis, radix puerariae, edible tulip and radix bupleuri. The traditional Chinese medicine mask disclosed by the invention is simple in preparation method, wide in raw material medicine sources, low in cost and free from toxic and side effects, and has obvious curative effects of whitening and lightening spots, tendering skin, eliminating acnes and acne marks.

Description

Traditional Chinese medicine mask for whitening and acne removal

Technical Field

The invention relates to the field of traditional Chinese medicines, in particular to a traditional Chinese medicine mask for whitening and removing acnes.

Background

With the improvement of living standard, people pay more attention to external images, and the clean and clean skin is healthy, pleasant and can increase confidence. However, with various environmental problems such as strong radiation, environmental pollution, population aging, global temperature rise, etc. of electronic products, skin pigment disorder diseases occur to more and more people. 80% of asian women have been investigated for fair and crystal skin; 70% of European women use whitening products to reduce and eliminate chloasma.

Tyrosinase is in melanin cells, and when stimulated by ultraviolet rays, emotion, pressure and other factors, can activate and catalyze tyrosine in blood to generate grey DOPA (DOPA) through oxidation reaction, and then black grey Dopaquinone (DQ) is generated, the dopaquinone is polymerized to form melanin particles, and the melanin particles are transferred to the skin surface layer under the action of cell metabolism. The whole process is affected by various enzymes such as tyrosinase, dopa tautomerase, DHICA oxidase, etc., and furthermore, the transfer of melanin is also affected by the morphology of the black pigment cells. Therefore, the whitening mainly has the following ways: 1. inhibit proliferation of melanocytes; 2. inhibiting the activity of the enzyme of interest to reduce melanin synthesis levels; 3. preventing transfer of melanin granules to the keratinocytes; 4. accelerating the metabolism speed of melanin; 5. reducing acceleration of melanin formation by exogenous stimuli such as oxygen radicals, ultraviolet rays, etc.

The production of acne inflammation and propionibacterium acnes (Propionibacterium acnes,P.acnes) Inducing an immune response to acne and releasing a range of inflammatory factors. Among them, inflammatory factors such as interleukin (IL-1. Beta.) and tumor necrosis factor (TNF-. Alpha.) play an important role in the inflammatory reaction of acne, and the content of these factors is also related to the severity of acne inflammation.

However, most of the common whitening and acne removing formulas adopt chemical synthesis preparations, so that the effect is limited, and adverse side effects are easy to generate in the chemical synthesis preparations. Therefore, it is urgent to find whitening additives with better effect, safer, richer resources and simpler acquisition.

The action mechanism of the facial mask comprises two aspects, namely isolating the outside air and pollution, improving the skin temperature, expanding skin pores, promoting secretion and metabolism of sweat glands, discharging the metabolism products of epidermal cells and accumulated oil and fat, and enabling the functional components in the facial mask to permeate into the stratum corneum, thereby achieving the purposes of cleaning, moisturizing, whitening, resisting wrinkles, removing acnes and the like. On the other hand, dirt, cellular metabolites and excessive grease on the skin are removed when the mask is peeled off after drying. The traditional Chinese medicine components with different effects are added into the facial mask to achieve the effects of moisturizing, whitening, removing freckles, removing acnes and the like. Although some natural traditional Chinese medicine mask products with main effects of whitening and acne removing appear in the current mask market, the traditional Chinese medicine mask has the defects of slow effect and limited whitening and acne removing effects, and the traditional Chinese medicine mask with obvious whitening and acne removing effects still has wide market prospect.

Disclosure of Invention

The invention aims to provide a traditional Chinese medicine mask capable of effectively whitening and removing acnes.

In order to achieve the above purpose, the present invention adopts the following technical scheme:

the first aspect of the invention provides a traditional Chinese medicine mask for whitening and acne removal, which is prepared from radix angelicae, rose, semen cuscutae, concha haliotidis, poria cocos, coix seed, rhizoma typhonii, bletilla striata, radix ampelopsis, radix puerariae, edible tulip and radix bupleuri.

Preferably, the traditional Chinese medicine mask is prepared from 1-30 parts by weight of dahurian angelica root, 1-30 parts by weight of rose, 5-30 parts by weight of semen cuscutae, 1-30 parts by weight of concha haliotidis, 5-40 parts by weight of poria cocos, 10-50 parts by weight of coix seed, 1-30 parts by weight of rhizoma typhonii, 1-30 parts by weight of bletilla striata, 5-30 parts by weight of Japanese ampelopsis root, 5-40 parts by weight of kudzuvine root, 5-30 parts by weight of edible tulip and 1-30 parts by weight of bupleurum root.

More preferably, the traditional Chinese medicine mask is prepared from 5-20 parts by weight of dahurian angelica root, 5-20 parts by weight of rose, 10-20 parts by weight of semen cuscutae, 5-20 parts by weight of concha haliotidis, 10-30 parts by weight of poria cocos, 20-40 parts by weight of coix seed, 5-20 parts by weight of rhizoma typhonii, 5-20 parts by weight of bletilla striata, 10-20 parts by weight of Japanese ampelopsis root, 10-30 parts by weight of kudzuvine root, 10-20 parts by weight of edible tulip and 5-20 parts by weight of bupleurum root.

Most preferably, the traditional Chinese medicine mask is prepared from 10 parts by weight of radix angelicae, 10 parts by weight of rose, 15 parts by weight of semen cuscutae, 10 parts by weight of concha haliotidis, 20 parts by weight of poria cocos, 30 parts by weight of coix seed, 10 parts by weight of rhizoma typhonii, 10 parts by weight of bletilla striata, 15 parts by weight of radix ampelopsis, 20 parts by weight of radix puerariae, 15 parts by weight of edible tulip and 10 parts by weight of radix bupleuri.

Preferably, the traditional Chinese medicine facial mask further comprises honey.

The second aspect of the present invention provides a method for preparing the above traditional Chinese medicine mask, comprising the following steps:

(1) Weighing radix Angelicae Dahuricae, flos Rosae Rugosae, semen Cuscutae, concha Haliotidis, poria, coicis semen, rhizoma Typhonii, rhizoma Bletillae, radix Ampelopsis, radix Puerariae, pseudobulbus Cremastrae seu pleiones and bupleuri radix, mixing, pulverizing into superfine powder, and sieving with 300-500 mesh sieve;

(2) Adding boiled water into the medicinal powder, blending into paste, cooling, adding appropriate amount of Mel, and mixing.

Preferably, the very fine powder in step (1) of the process is sieved through a 400 mesh sieve.

Preferably, in step (2) of the method, honey is added in an amount of 1ml/5g of the powder.

Preferably, the melanoma cells are B16 melanoma cells.

The traditional Chinese medicine raw materials selected by the invention have the following common pharmacological effects in the field:

dahurian angelica root, radix Angelicae Dahuricae, warm in nature, pungent in flavor, enters lung, spleen and stomach meridians. Radix Angelicae Dahuricae has effects of relieving exterior syndrome, dispelling cold, dispelling pathogenic wind, relieving pain, relieving stuffy nose, eliminating dampness, stopping leukorrhagia, relieving swelling, expelling pus, dispelling pathogenic wind, and relieving itching, and can be used for treating common cold due to wind-cold, headache, toothache, rheumatalgia, nasosinusitis, leukorrhagia, sore, carbuncle, and toxic swelling.

The rose is sweet in taste and slightly bitter in flavor, and enters liver meridian and spleen meridian. The flos Rosae Rugosae has effects of activating qi-flowing, resolving stagnation, regulating blood, and relieving pain, and can be used for treating liver and stomach qi pain, anorexia, nausea, menoxenia, traumatic injury.

Semen Cuscutae has mild property and pungent and sweet taste, and enters liver, kidney and spleen meridians. Semen cuscutae has effects of tonifying liver and kidney, securing essence, reducing urination, preventing miscarriage, improving eyesight, relieving diarrhea, and dispelling pathogenic wind and removing speckle, and can be used for treating spermatorrhea, dysuria, enuresis, frequent urination, vitiligo, etc.

Shi Jue Ming is cold in nature, salty in taste and enters liver meridian. Concha Haliotidis has effects of suppressing hyperactive liver, subsiding yang, removing liver fire, improving eyesight, and can be used for treating headache, giddiness, conjunctival congestion, nebula, blurred vision, and blue-blind sparrow eyes.

Poria, with flat nature, sweet and bland taste, enters heart, lung, spleen and kidney meridians. Poria has effects of promoting diuresis, removing dampness, invigorating spleen, and calming heart, and can be used for treating edema, oliguria, phlegm retention, palpitation, spleen deficiency, anorexia, loose stool, diarrhea, uneasiness, palpitation, and insomnia.

Yi ren is cool in nature, sweet and light in taste, and enters spleen, stomach and lung meridians. Semen Coicis has effects of promoting diuresis, removing dampness, invigorating spleen, relieving diarrhea, removing arthralgia, expelling pus, removing toxic materials, and resolving hard mass, and can be used for treating edema, tinea pedis, dysuria, spleen deficiency diarrhea, wet arthralgia, pulmonary abscess, and intestinal abscess; warts, cancers.

Bai Fu Zi is warm in nature and pungent in flavor, enters stomach meridian and liver meridian. Rhizoma Typhonii has effects of dispelling pathogenic wind and phlegm, arresting convulsion, removing toxic substance, resolving hard mass, and relieving pain, and can be used for treating apoplexy, facial distortion, dysphoria, phlegm syncope headache, migraine, sore throat, and tetanus; it is indicated for scrofula and phlegm nodule and venomous snake bite.

Bletilla striata, also known as bletilla striata, is slightly cold in nature, bitter in taste, sweet in flavor, astringent in taste, and enters lung meridian, liver meridian and stomach meridian. The rhizoma Bletillae has effects of astringing, stopping bleeding, relieving swelling, and promoting granulation, and can be used for treating traumatic hemorrhage, pyocutaneous disease, toxic swelling, and chapped skin.

Ampelopsis root, radix Ampelopsis, with slightly cold nature, is bitter in flavor. It enters heart meridian and stomach meridian. The ampelopsis japonica has the effects of clearing heat and detoxicating, and resolving carbuncles and resolving hard masses, and is used for treating carbuncles, furuncles, scrofula, burns and scalds, and the like.

Pueraria root, radix Puerariae, with flat nature, sweet and pungent taste, enters spleen and stomach meridians. Radix Puerariae has effects in relieving exterior syndrome, promoting eruption, promoting salivation, and relieving diarrhea, and can be used for treating common cold, fever, aversion to cold, no sweat, and strong neck.

Shi Gao is cool in nature, sweet in flavor, slightly pungent in flavor, and enters liver meridian and spleen meridian. The edible tulip has the effects of clearing heat and detoxicating, resolving phlegm and resolving masses, and can be used for treating carbuncle, furuncle, scrofula, tuberculosis of lymph nodes, and snake and insect bites.

Bupleuri radix, with slightly cold nature, pungent and bitter taste, enters liver, gallbladder and lung meridians. Bupleuri radix and relieving exterior syndrome, dispersing stagnated liver qi for relieving qi stagnation, invigorating yang, and relieving heat and malaria, and can be used for treating common cold, fever, distending pain in chest and hypochondrium, menoxenia, uterine prolapse, and rectocele.

The invention has the positive beneficial effects that:

1. the traditional Chinese medicine mask disclosed by the invention adopts natural traditional Chinese medicine raw materials, and is simple in preparation method, wide in raw material medicine sources, low in cost and free from toxic and side effects.

2. The traditional Chinese medicine mask disclosed by the invention is scientific and reasonable in compatibility of the prescription, and the raw materials are different in effect and supplement each other, so that the traditional Chinese medicine mask has obvious curative effects of whitening and lightening spots, tendering skin, eliminating acnes and acne marks, and has less adverse reaction during the administration.

Detailed Description

The present invention will be further described with reference to examples, but the embodiments of the present invention are not limited thereto. The experimental methods used in the following examples are conventional methods unless otherwise specified.

Example 1

Weighing 10g of radix angelicae, 10g of rose, 15g of semen cuscutae, 10g of concha haliotidis, 20g of poria cocos, 30g of semen coicis, 10g of rhizoma typhonii, 10g of bletilla striata, 15g of radix ampelopsis, 20g of radix puerariae, 15g of edible tulip and 10g of radix bupleuri, mixing, grinding into superfine powder by a machine, sieving with a 300-500-mesh sieve, blending with boiled water to form paste, cooling, adding 35ml of honey, and uniformly mixing to obtain the traditional Chinese medicine mask 1.

Example 2

Weighing 20g of radix angelicae, 20g of rose, 15g of semen cuscutae, 10g of concha haliotidis, 20g of poria cocos, 10g of semen coicis, 10g of rhizoma typhonii, 10g of bletilla striata, 15g of radix ampelopsis, 20g of radix puerariae, 15g of edible tulip and 10g of radix bupleuri, mixing, grinding into superfine powder by a machine, sieving with a 300-500-mesh sieve, blending with boiled water to form paste, cooling, adding 35ml of honey, and uniformly mixing to obtain the traditional Chinese medicine mask 2.

Example 3

Weighing 10g of radix angelicae, 10g of rose, 15g of semen cuscutae, 10g of concha haliotidis, 20g of poria cocos, 10g of semen coicis, 20g of rhizoma typhonii, 20g of bletilla striata, 15g of radix ampelopsis, 20g of radix puerariae, 15g of edible tulip and 10g of radix bupleuri, mixing, grinding into superfine powder by a machine, sieving with a 300-500-mesh sieve, blending with boiled water to form paste, cooling, adding 35ml of honey, and uniformly mixing to obtain the traditional Chinese medicine mask 3.

Example 4

Weighing 10g of radix angelicae, 10g of rose, 15g of semen cuscutae, 10g of concha haliotidis, 20g of poria cocos, 30g of semen coicis, 10g of rhizoma typhonii, 10g of bletilla striata, 25g of radix ampelopsis, 10g of radix puerariae, 15g of edible tulip and 10g of radix bupleuri, mixing, grinding into superfine powder by a machine, sieving with a 300-500-mesh sieve, blending with boiled water to form paste, cooling, adding 35ml of honey, and uniformly mixing to obtain the traditional Chinese medicine mask 4.

Example 5

Weighing 20g of radix angelicae, 10g of rose, 15g of semen cuscutae, 10g of concha haliotidis, 20g of poria cocos, 30g of coix seed, 10g of rhizoma typhonii, 10g of bletilla striata, 15g of radix ampelopsis, 10g of radix puerariae, 15g of edible tulip and 10g of radix bupleuri, mixing, grinding into superfine powder by a machine, sieving with a 300-500-mesh sieve, blending with boiled water to form paste, cooling, adding 35ml of honey, and uniformly mixing to obtain the traditional Chinese medicine mask 5.

The mask of the embodiments 1-5 of the present invention is used as follows: the traditional Chinese medicine mask is externally applied to the face, the preservative film is covered on the outer layer for moisturizing, and after 20 minutes, the face is basically scraped by a scraping plate and then cleaned by warm water; the frequency of use is once a week.

Test example 1, chinese medicine pair of the inventionP.acnesTreatment of induced mouse ear inflammation

1. Test method

1.1、P.acnesSuspensionPreparation

SelectingP.acnesCulturing strain in brain heart infusion culture medium, activating and proliferating, collecting colony, filtering, and regulating bacterial liquid concentration to 5×10 ¹² CFU/L. PreparingP.acnesSuspension for establishingP.acnesMice were injected intradermally with a model of otic inflammation.

1.2 preparation of decoction of Chinese herbs

Taking 175g of the traditional Chinese medicine composition of the embodiment 1-5 of the invention respectively, mixing, grinding into superfine powder by a machine, sieving by a 300-500-mesh sieve, adding distilled water with the weight being 2 times that of the traditional Chinese medicine composition, decocting, filtering, and concentrating filtrate into 1g/mL of traditional Chinese medicine decoction for 1-5 for later use.

1.3 dosing regimen

70 healthy mice were selected and randomly divided into 7 groups (i.e., control group, model group, examples 1-5 of the present invention), 10 each. Wherein, the control group is injected with 20 mu L of PBS solution in the center of the double-sided inner auricle of the mouse, and the model group is injected in the center of the double-sided inner auricle of the mouseP.acnes20. Mu.L of suspension; the modeling method of the embodiment 1-5 groups is the same as that of the model group, after modeling is carried out for 5 days, the embodiment groups smear Chinese medicinal decoction 1-5 on the auricles of the mice, the Chinese medicinal decoction is continuously administrated for 14d for 2 times per day and 0.1mL each time, the mice are killed after the last administration for 24 hours, the serum of the mice is collected, the corresponding operation is carried out according to the description of a kit by an ELISA method, and the levels of IL-1 beta and TNF-alpha are detected.

2. Test results

The IL-1. Beta. And TNF-alpha. Content in the serum of mice treated with the traditional Chinese medicines of each test group of the present invention is shown in Table 1 below.

TABLE 1 Effect of the inventive Chinese herbs in each test group on IL-1 beta and TNF-alpha levels in mouse serum

Group of	IL-1β（ng/L）	TNF-ɑ（ng/L）
			Control group	42.82±5.87	15.31±2.69
Model group	147.27±11.03	57.32±4.73
			Example 1 group	62.56±5.02	24.09±2.34
Example 2 group	88.40±6.25	37.29±2.94
			Example 3 group	84.56±8.21	38.95±2.76
Example 4 group	92.42±7.98	42.41±3.98
			Example 5 group	102.34±8.37	40.02±4.56

As can be seen from Table 1 above, the serum IL-1. Beta. And TNF-alpha. Levels in mice in the model group were significantly elevated compared to the control group, confirmingP.acnesThe intradermal injection mice have a successful model of otic inflammation. And modelCompared with mice in groups, the IL-1 beta and TNF-alpha levels in the serum of the mice can be reduced to different degrees in all the groups 1 to 5 of the invention, and the difference has statistical significance (P)<0.05 The effective components of the traditional Chinese medicine mask are aimed atP.acnesThe induced mouse ear inflammation has definite anti-inflammatory effect, which indicates that the traditional Chinese medicine mask provided by the invention can be clinically used for treating acne and has the acne removing effect. Notably, the reduction of IL-1 beta and TNF-alpha levels in the serum of mice was most pronounced in group 1 of the present invention, suggesting that the traditional Chinese medicine composition may produce excellent anti-inflammatory and acne treatment effects.

Experimental example 2 biological Effect of the Chinese medicine of the invention on B16 mouse melanoma cells

1. Test method

1.1 preparation of Chinese medicinal decoction

Taking 175g of the traditional Chinese medicine composition of the embodiment 1-5 of the invention respectively, mixing, grinding into superfine powder by a machine, sieving by a 300-500-mesh sieve, adding distilled water with the weight being 2 times that of the traditional Chinese medicine composition, decocting, filtering, and concentrating filtrate into 1g/mL of traditional Chinese medicine decoction for 1-5 for later use. Before use, the solution is diluted to 1mg/mL with distilled water.

1.2, B16 melanoma cell culture

Aseptically inoculating B16 melanoma cells into RPMI-1640 medium containing 10% foetal calf serum, culturing in carbon dioxide incubator at 37deg.C and 5% CO ₂ Culturing in saturated humidity environment, and changing liquid every day. When the cells grow to 80% -90% confluence, the cells are digested and passaged by 0.25% trypsin, and the cells are continuously cultured. Each experiment was taken from the same passage of cells, with an initial seed cell concentration of 10 per ml of culture medium ⁵ About a single cell.

1.3 detection of cell proliferation Rate by MTT colorimetric method

Taking B16 melanoma cells in exponential growth phase, preparing single cell suspension by digestion and dispersion, regulating cell density to 1×10 per well ⁴ The cell density of each cell is inoculated in a 96-well plate, the cell is cultivated for 12 hours, a new culture medium is replaced after the cell is completely attached, 100 mu L of 1mg/mL of Chinese medicinal decoction with the concentration of 1 to 5 are respectively added into each well in the groups of examples 1 to 5, 10 holes are repeated in each test group, and fresh culture solution is added into the control group to replace the Chinese medicinal decoctionAfter 48h incubation, 50. Mu.L of MTT solution of 1mg/mL was added to each well, incubated in an incubator for 4h, medium and MTT were discarded, 150. Mu.L of DMSO was added to each well to dissolve residual MTT-formazan crystals, and absorbance at 570nm was measured for each well with an microplate reader after shaking and mixing.

The cell proliferation rate was calculated according to the following formula: cell proliferation rate = (experimental well OD value-blank well OD value)/(control well OD value-blank well OD value) ×100%.

1.4 influence of the Chinese traditional medicine of the invention on melanin synthesis

Cell culture and experimental treatments were as described in section 1.3. Culturing for 48h, wherein the cells are grown to 80% -90% fusion, discarding the supernatant, washing with PBS for 2 times, digesting and collecting 2mL of total cells in a centrifuge tube, diluting 20 μL by a certain multiple for cell counting, centrifuging at 1500r/min for 10min, and discarding the supernatant. 2mL of PBS was added for resuspension, then 500. Mu.L of ethanol diethyl ether mixture with a volume ratio of 1:1 was added, the mixture was left at room temperature for 30min, centrifuged at 3000r/min for 5min, the supernatant was discarded, 1mL of 1mol/L NaOH solution containing 10% DMSO was added, water bath was conducted at 80℃for 45min, and the absorbance at 405nm was measured.

Total melanin synthesis = experimental OD/control OD x 100%.

2. Test results

The effect of the present invention on B16 melanoma cell proliferation rate and total melanin synthesis in each test group is shown in Table 2 below.

TABLE 2 Effect of the inventive Chinese medicine on proliferation Rate of B16 melanoma cells and total amount of melanin synthesis

Group of	Cell proliferation Rate (%)	Total melanin synthesis (%)
			Example 1 group	28.37±3.88	16.24±2.67
Example 2 group	43.29±5.02	30.92±3.08
			Example 3 group	48.01±5.28	32.46±2.90
Example 4 group	50.42±4.91	40.57±3.89
			Example 5 group	49.26±5.90	36.25±3.47

The results in Table 2 show that the active ingredients of the traditional Chinese medicines in the examples 1-5 can obviously inhibit the growth of B12 melanocytes and the synthesis of total melanin in the B12 cells, and the inhibition effect of the group in the example 1 on the total melanin synthesis and the proliferation of cells is obviously stronger than that of the group in the examples 2-5. In conclusion, the active ingredients of the traditional Chinese medicine mask disclosed by the invention can simultaneously inhibit melanin generation from two aspects of reducing melanin synthesis sites (namely inhibiting cell proliferation) and reducing the synthesis amount of melanin in cells, so that the traditional Chinese medicine mask disclosed by the invention is predicted to have a whitening effect clinically.

It is to be understood that the above-described embodiments of the present invention are merely illustrative of or explanation of the principles of the present invention and are in no way limiting of the invention. Accordingly, any modification, equivalent replacement, improvement, etc. made without departing from the spirit and scope of the present invention should be included in the scope of the present invention.

Claims

1. The application of the traditional Chinese medicine composition in preparing the acne-removing traditional Chinese medicine mask is characterized in that the preparation method of the traditional Chinese medicine composition is as follows: weighing 10g of radix angelicae, 10g of rose, 15g of semen cuscutae, 10g of concha haliotidis, 20g of poria cocos, 30g of semen coicis, 10g of rhizoma typhonii, 10g of bletilla striata, 15g of radix ampelopsis, 20g of radix puerariae, 15g of edible tulip and 10g of radix bupleuri, mixing, grinding into superfine powder by a machine, sieving with a 300-500-mesh sieve, blending with boiled water to form paste, cooling, adding 35ml of honey, and uniformly mixing.

2. The application of the traditional Chinese medicine composition in preparing the whitening traditional Chinese medicine mask is characterized in that the preparation method of the traditional Chinese medicine composition is as follows: weighing 10g of radix angelicae, 10g of rose, 15g of semen cuscutae, 10g of concha haliotidis, 20g of poria cocos, 30g of semen coicis, 10g of rhizoma typhonii, 10g of bletilla striata, 15g of radix ampelopsis, 20g of radix puerariae, 15g of edible tulip and 10g of radix bupleuri, mixing, grinding into superfine powder by a machine, sieving with a 300-500-mesh sieve, blending with boiled water to form paste, cooling, adding 35ml of honey, and uniformly mixing.