CN114747657A

CN114747657A - Preparation method of composite microbial feed additive and special equipment thereof

Info

Publication number: CN114747657A
Application number: CN202210412610.7A
Authority: CN
Inventors: 龙光祥
Original assignee: Nanning Aonong Feed Co ltd
Current assignee: Nanning Aonong Feed Co ltd
Priority date: 2022-04-19
Filing date: 2022-04-19
Publication date: 2022-07-15

Abstract

The invention relates to the technical field of animal feed processing, in particular to a compound microorganism feed additive, a preparation method thereof and special equipment. The composite microbial metabolite is obtained by culturing lactobacillus plantarum and lactobacillus casei to obtain bacterium powder A, culturing bacillus subtilis to obtain bacterium powder B, and compounding the bacterium powder A and the bacterium powder B according to a certain proportion, so that the daily gain of the bred animals can be effectively increased, and the feed conversion ratio is reduced. By providing the fluid nozzle in the spray drying apparatus, the liquid cell located in the middle is drawn into a thin layer by the impact of the compressed gas on both sides, and when the liquid cell collides with the nozzle edge, the liquid cell can form a spray droplet having a small particle diameter.

Description

Preparation method of composite microbial feed additive and special equipment thereof

Technical Field

The invention belongs to the technical field of animal feed processing, and particularly relates to a preparation method of a compound microbial feed additive and special equipment.

Background

With the rapid development of livestock and poultry breeding in China, modern large-scale breeding is a great trend of livestock and poultry breeding, and scientific and technical breeding also has a wider application space. However, the potential risk formed for a long time is not properly solved due to the pure pursuit of breeding benefits in the livestock and poultry breeding industry, the drug residues in the bodies of animals and the mass propagation of drug-resistant pathogenic bacteria caused by the long-term abusing of various antibiotics have great threat to the health of people, and the feeding microbial preparation is gradually accepted by the livestock and poultry breeding world due to the advantages of no toxic or side effect, no residues in the bodies, no pollution, no drug resistance, low cost, obvious effect and the like. The intestinal microorganisms interact with the environment in a host, the microbial preparation with a single microbial inoculum type easily loses activity and function due to the change of the environment in an animal body, the production performance is unstable, the microbial preparation reaches the high-bile-salt environment of the intestinal tract through the strong acid environment of the stomach and is greatly inactivated, the number of the surviving microorganisms is small, and the probiotic activity of the microorganisms is hardly exerted after the microorganisms reach the intestinal tract, so that the product quality of the microbial preparation is influenced. Therefore, it is a focus of attention to select a microbial feed additive consisting of probiotics, which enhances the gastrointestinal tract regulatory ability of the probiotics through the selection combination of the probiotics.

The Chinese patent with the application number of 2019102789062 discloses a compound microbial feed additive and a preparation method thereof, the compound microbial feed additive is prepared from lactobacillus fermentation liquor, bacillus mucilaginosus fermentation liquor, trichoderma longibrachiatum fermentation liquor and chlorella algae liquor, the provided compound microbial feed additive can improve the intestinal function of piglets, improve the daily weight gain and the feed conversion efficiency of weaned piglets, has remarkable antibacterial and antiviral effects, can remarkably improve the immunity of the piglets, effectively prevents the piglets from diarrhea after weaning, and has wide application prospect.

The Chinese patent with the application number of 2010102092866 discloses a compound microorganism feed additive for chicks and a preparation method thereof, wherein the feed additive comprises probiotics and prebiotics, the probiotics comprise bifidobacterium, lactobacillus acidophilus, enterococcus faecium and bacillus licheniformis, and the prebiotics comprise xylo-oligosaccharide. The feed additive can effectively reduce the morbidity of chicks, effectively improve daily gain and feed-weight ratio, reduce production cost, effectively reduce the use amount of antibiotics and improve the quality of livestock products.

The Chinese patent with the application number of 2013106938694 discloses a microbial feed additive and a preparation method thereof, wherein a liquid fermentation culture medium left in the production of biological cellulose is used as a raw material, wastes are utilized, resources are saved, and environmental pollution is reduced; the microbial feed additive containing live bacteria can be prepared by adding animal blood powder and a complex enzyme preparation and inoculating compound beneficial microbes; in addition, residual biological cellulose exists in a residual liquid fermentation culture medium produced by the biological cellulose, and the residual biological cellulose is a good dietary fiber source of animals and can play a role in balancing dietary nutrition and improving the intestinal health of the animals together with beneficial compound microorganisms.

However, the prior art does not relate to how to improve the survival rate of beneficial strains in the culture process, so that a preparation method of the compound microbial feed additive is needed to improve the production quality.

Disclosure of Invention

The invention aims to provide a compound microbial feed additive with high beneficial bacterium activity, which can improve the production efficiency, increase the number of beneficial bacteria and reduce the damage or death of the beneficial bacteria.

The technical scheme adopted by the invention for realizing the purpose is as follows:

a preparation method of a compound microorganism feed additive is characterized in that,

the compound microbial feed additive consists of two different layered compound microbial flora powders:

and (3) fungus powder A: 50% of lactobacillus plantarum and 50% of lactobacillus casei;

and (3) fungus powder B: 100% of bacillus subtilis;

the preparation method of the fungus powder A comprises the following steps:

s1, inoculating lactobacillus plantarum and lactobacillus casei to an MRS culture medium, namely 10g of peptone, 10g of beef extract, 5g of yeast extract powder, 20g of glucose, 801.0 mL of tween-801.0, 2g of dipotassium phosphate, 5g of sodium acetate, 2g of triammonium citrate, 0.2g of magnesium sulfate, 0.05g of manganese sulfate, 20g of agar powder and 1000mL of distilled water, sterilizing at 121 ℃ for 20min, and culturing to obtain a seed solution;

S2, inoculating the seed solution to a fermentation medium, namely 3.6kg of peptone, 2.4kg of yeast extract, 0.6kg of beef extract, 3.2kg of glucose, 0.06kg of magnesium sulfate, 1.2kg of triammonium citrate, 1.8kg of sodium acetate, 600L of distilled water, sterilizing at 121 ℃ for 15min to obtain fermentation liquor;

s3, after the fermentation is finished, concentrating the fermentation liquor by using a membrane concentrator, and collecting the concentrated liquor;

s4, adding 0.2kg of skimmed milk powder, 1.2kg of lactose and 0.1kg of sodium alginate into the concentrated solution, and uniformly stirring to obtain a fungus body fluid A;

and S5, carrying out vacuum low-temperature drying through spray drying by a vacuum low-temperature dryer to obtain the product.

The invention also provides special production equipment for the composite microbial feed additive, which comprises a spray drying device, wherein an atomizing device in the spray drying device can form micron-sized spray droplets, and the spray drying device comprises:

a first fluid nozzle and a second fluid nozzle symmetrically disposed about the nozzle;

a first passage between the first fluid nozzle and the nozzle, a second passage between the second fluid nozzle and the nozzle;

a third fluid nozzle located on the first channel and in communication with the first channel, a fourth fluid nozzle located on the second channel and in communication with the second channel;

The first channel is a columnar structure with the width gradually reduced from an inlet to the nozzle, and the second channel comprises a gradually-reduced section with the width gradually reduced close to the inlet of the second channel and a uniform section with the width unchanged close to one side of the nozzle;

the fourth fluid nozzle is arranged on the gradual change section of the second channel, a triangular control plate is arranged near the connection part of the gradual change section and the uniform section, the triangular control plate is connected on the inner wall of one side, close to the fourth fluid channel, of the second channel, the triangular control plate is in a right-angled triangle shape and comprises a tip extending to the gradual change section and a horizontal end just contacting with the inlet end of the uniform section, at least a first channel plate and a second channel plate are arranged on the bevel edge section of the triangular control plate, the directions of the first channel plate and the second channel plate are parallel to the axial direction of the second channel, guide rails are arranged on the two outer sides of the first channel plate and the second channel plate, a driving part capable of driving the first channel plate and the second channel plate to move is arranged on the guide rails, and the distance between the first channel plate and the second channel plate can be controlled by driving the driving part through a control part, a triangular plate is fixedly arranged at the center position below the horizontal end through a rotating shaft, the triangular plate is triangular, the pointed end of the triangular plate faces the horizontal end, and the triangular plate can be driven by a rotating motor to rotate;

The end part of the first channel close to the nozzle is in a quadrangular pyramid shape, the end part of the second channel close to the nozzle is in a quadrangular prism shape, the length and the width of the inlet of the first fluid channel are the same as those of the inlet of the second fluid channel, and the length of the first channel is the same as that of the second channel.

The invention has the following beneficial effects:

1. the lactobacillus plantarum has higher viable count compared with other lactobacillus, can produce special lactobacillus in the reproduction process, can regulate immunity, inhibit pathogenic bacteria, maintain the balance of flora in intestinal tracts and promote the absorption of nutrient substances; after entering an animal body, lactobacillus casei can survive in a large amount in intestinal tracts and can effectively regulate the balance of intestinal flora; the bacillus subtilis has stronger stability in particles or powder, can smoothly enter large and small intestinal tracts of animals for survival and can be propagated in a large range; by compounding the bacterial powder A obtained by culturing the lactobacillus plantarum and the lactobacillus casei and the bacterial powder obtained by culturing the bacillus subtilis according to a certain proportion, the daily gain of the fed animals can be effectively increased, and the feed conversion ratio is reduced;

2. the composite microbial feed additive prepared by the special equipment can realize a layered composite structure of the thalli A and the thalli B, and the composite microbial feed additive is at least positioned on the outer layer and mainly made of the thalli B, so that the stability of the composite microbial feed additive is improved, the survival rate of beneficial bacteria is improved, and the survival rate of the composite microbial feed additive passing through the stomach is further improved, more importantly, the bacterium liquid B can flow through the tip of the triangular control plate, the direction of the thin layer of the bacterium liquid B can be changed by controlling the distance between the first channel plate and the second channel plate, meanwhile, part of the bacterium liquid is scattered in the thin layer manner, so that most of the bacterium liquid flows in through the first channel plate, the rest part of the bacterium liquid enters through the second channel plate, and the tip of the rotating shaft is positioned between the first channel plate and the second channel plate by adjusting the rotating motor, therefore, the bacteria liquid B can be layered through the arrangement, the uniform section arrangement enables the layered arrangement to be maintained until the bacteria liquid B enters the nozzle, most of the bacteria liquid B on the inner side of the nozzle collides with the bacteria liquid A through a lamellar structure to form spray liquid drops with smaller particle sizes, after the liquid drops are formed through collision, the bacteria liquid B on the lower side of the bacteria liquid B is coated through the layered structure on the lower side of the triangle, the thickness and compactness of the coating layer of the bacteria liquid B can be controlled according to actual requirements by controlling the position between the triangle and the first and second channel plates, the higher the flow speed of the lower layer is, the higher the compactness and thickness of the lower layer are, the damage or death of beneficial bacteria is effectively reduced by the formed composite microbial feed additive, and when the formed spray liquid drops enter the drying chamber, the moisture contained in the spray liquid drops is quickly gasified at the entrance of the drying chamber, the heat is absorbed, the temperature in the drying chamber is reduced, and the damage or death of beneficial bacteria is effectively reduced.

Drawings

FIG. 1 is a schematic view of a spray drying apparatus according to the present invention;

FIG. 2 is a schematic structural diagram of a triangular control plate;

FIG. 3 is a schematic view of a triangle;

FIG. 4 is an enlarged view taken at A of FIG. 1;

FIG. 5 is an enlarged view of FIG. 1 at B;

fig. 6 is an enlarged schematic view of fig. 2 at C.

In the figure, 1, a first fluid nozzle; 2. a second fluid nozzle; 3. a third fluid nozzle; 4. a fourth fluid nozzle; 5. a triangular control plate; 6. a first channel plate; 7. a second channel plate; 8. a guide rail; 9. a drive member; 10. a set square; 11. a rotating shaft; 12. rotating the motor; 13. a nozzle; 14. a square outlet; 15. a transition section; 16. and (5) homogenizing.

Detailed Description

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

Example 1

Preparation of fungus powder A:

s1, culturing of Lactobacillus plantarum and Lactobacillus casei seeds:

Inoculating lactobacillus plantarum and lactobacillus casei to an MRS culture medium, culturing at 35-2 ℃ for 12-20 h to obtain a seed solution, wherein the culture medium is as follows: 10g of peptone, 10g of beef extract, 5g of yeast extract powder, 20g of glucose, 801.0 mL of tween-801.0, 2g of dipotassium phosphate, 5g of sodium acetate, 2g of ammonium citrate tribasic, 0.2g of magnesium sulfate, 0.05g of manganese sulfate, 20g of agar powder and 1000mL of distilled water, wherein the pH value is 6.4-6.8, and the sterilization is carried out by high-pressure steam at 121 ℃ for 20 min.

S2, fermenting and culturing the lactobacillus plantarum and the lactobacillus casei:

inoculating the seed liquid to a fermentation culture medium, wherein the culture temperature is 34 +/-1 ℃, and the culture time is 12-16 h to obtain a fermentation liquid; wherein the culture medium is: 3.6kg of peptone, 2.4kg of yeast extract, 0.6kg of beef extract, 3.2kg of glucose, 0.06kg of magnesium sulfate, 1.2kg of ammonium citrate, 1.8kg of sodium acetate, 600L of distilled water, 6.0-6.5 of pH, and high-pressure steam sterilization at 121 ℃ for 15 min.

S3, after the fermentation is finished, concentrating the fermentation liquor by using a membrane concentrator, and collecting 32L of concentrated liquor;

s4, adding 0.2kg of skimmed milk powder, 1.2kg of lactose and 0.1kg of sodium alginate into the concentrated solution, and fully stirring until the mixture is uniformly stirred to obtain a bacterial fluid A;

s5, carrying out vacuum low-temperature drying through spray drying by a vacuum low-temperature dryer, wherein the drying temperature is 34 +/-2 ℃, and the vacuum degree is 500-.

Example 2

Preparation of fungus powder B:

t1, culturing the bacillus subtilis seeds:

inoculating the bacillus subtilis to an LB culture medium, culturing at the temperature of 37 +/-0.5 ℃ for 12-18h, and culturing to obtain a seed solution, wherein the culture medium is as follows: 10g of peptone, 5g of yeast extract, 10g of NaCl, 1000ml of distilled water and 20g of agar, and sterilizing at 121 ℃ for 20 min.

T2, fermentation culture of bacillus subtilis:

inoculating the seed liquid into a fermentation culture medium, wherein the culture temperature is 35 +/-0.5 ℃, and the culture time is 12-18 h; the culture medium is as follows: 12kg of sucrose, 9kgL of peptone, 3kg of yeast extract powder, 0.6kg of magnesium sulfate, 1.2kg of monopotassium phosphate, 1.2kg of dipotassium phosphate and 600L of distilled water, adjusting the pH to 7.0, and sterilizing for 15min by high-pressure steam at 121 ℃.

T3, after the fermentation is finished, concentrating the fermentation liquor by using a membrane concentrator, and collecting 38L of concentrated solution;

t4, adding 0.15kg of skimmed milk powder, 1.2kg of lactose and 0.15kg of sodium alginate into the concentrated solution, and fully stirring until the mixture is uniformly stirred to obtain a bacterial fluid B;

t5, spray drying by a vacuum low-temperature dryer, and carrying out vacuum low-temperature drying at the drying temperature of 35 +/-2 ℃ and the vacuum degree of 400-.

Example 3

And compounding the bacterium powder A and the bacterium powder B according to the mass ratio of 1:1 to obtain the compound microbial metabolite, wherein the preparation method of the bacterium powder A refers to example 1, and the preparation method of the bacterium powder B refers to example 2.

Example 4

And (3) compounding the bacterium powder A and the bacterium powder B according to the mass ratio of 1:1.1 to obtain the compound microorganism metabolite, wherein the preparation method of the bacterium powder A refers to example 1, and the preparation method of the bacterium powder B refers to example 2.

Example 5

And (3) compounding the bacterium powder A and the bacterium powder B according to the mass ratio of 1:1.2 to obtain the compound microorganism metabolite, wherein the preparation method of the bacterium powder A refers to example 1, and the preparation method of the bacterium powder B refers to example 2.

Comparative example 1

Preparing lactobacillus plantarum powder:

the lactobacillus plantarum was inoculated in MRS medium and the rest of the preparation was the same as the preparation of powder a in example 1.

Comparative example 2

Preparation of lactobacillus casei powder:

lactobacillus casei was inoculated in MRS medium, and the rest of the preparation method was the same as that of powder A in example 1.

Feed efficacy verification

1. Broiler chicken raising experimental scheme

80 Aijia Yijia broilers with the age of 1 day are selected and randomly divided into 8 groups, a control group is fed with basic ration, different metabolite products subjected to spray drying are respectively added into the basic ration in a test group, the addition amount is 0.5 percent (mass ratio) to feed broilers, the test period is 35 days, and the test data is the average value of the whole feeding period. The test results are shown in table 1.

TABLE 1

As can be seen from the data in Table 1, the product obtained in example 5 has a significant effect in increasing the daily gain and the feed-meat ratio of broiler chickens compared with other groups, wherein the daily gain is increased by 17.82% compared with the control group, and the feed-meat ratio is 1.60.

2. Piglet feeding experimental scheme

80 Harbin white piglets are selected and randomly divided into 8 groups, the control group is fed with basic ration, different metabolite products subjected to spray drying are respectively added into the basic ration in the test group, the addition amount is 0.3 percent (mass ratio) to feed the white piglets, the test period is 30 days, and the test data is the average value of the whole feeding period. The test results are shown in table 2.

TABLE 2

As can be seen from the data in Table 2, the product obtained in example 5 has a significant effect in increasing the daily gain and the feed-meat ratio of the white piglets compared with other groups, wherein the daily gain is increased by 20.89% compared with the control group, and the feed-meat ratio is 1.72.

An atomizing device in a spray drying device can form micron-sized spray liquid drops 7. When the particle size of the spray droplets 7 is reduced, the surface area of the spray droplets 7 per unit mass is increased, so that the contact area with hot air can be effectively increased, and the damage or death of thalli caused by too long heating time of dry hot air can be effectively inhibited, therefore, when the micron-sized spray droplets 7 are formed, the contact area between the droplets formed by spraying and the hot air for drying is greatly increased, the drying effect can be improved, and the drying degree can be increased.

The spray drying device of the invention is provided with 4 fluid nozzles, as shown in figure 1, a first fluid nozzle 1 and a second fluid nozzle 2 are introduced with compressed gas, a third fluid nozzle 3 and a fourth fluid nozzle 4 are introduced with bacteria liquid, when the bacteria liquid A and the bacteria liquid B are introduced into the third fluid nozzle 3 or the fourth fluid nozzle 4, the compressed gas is introduced into the first fluid nozzle 1 and the second fluid nozzle 2, the compressed gas introduced from the first fluid nozzle 1 and the second fluid nozzle 2 flows on the surfaces of the bacteria liquid A and the bacteria liquid B at a high gas flow rate, under the impact of the high gas flow rate, the bacteria liquid A and the bacteria liquid B are stretched into a thin layer by impact when flowing through a channel surface 5, the collision occurs when passing through the edge tip of the nozzle 6, the stretched liquid is micronized by the impact force, micron-sized spray droplets 7 are formed, and the generated spray droplets enter a drying chamber, and (3) enabling dry hot air to contact with the spray liquid drops, and evaporating water in the spray liquid drops by heat generated by the hot air to finally obtain the thallus powder.

Wherein the compressed gas is one of air, carbon dioxide, nitrogen or argon. The introduction amount of the compressed gas is as follows: the gas is introduced into the nozzle 6 at a rate of about 1 to 100L/min.

The inlet temperature of the drying chamber is 200-250 ℃, when the spray droplets enter the drying chamber, the moisture contained in the spray droplets is gasified at the higher temperature of the inlet of the drying chamber, the liquid is gasified to absorb heat, the temperature in the drying chamber is lower than the temperature at the inlet of the drying chamber, the damage or death of beneficial bacteria is further reduced by shortening the retention time of the spray droplets in the drying chamber, and the outlet temperature of the drying chamber is 25-70 ℃.

Compared with other lactic acid bacteria, the lactobacillus plantarum has higher viable count, can produce a large amount of acid, can produce special lactobacillin in the propagation process, has a plurality of health-care functions, and can regulate immunity, inhibit pathogenic bacteria, maintain the balance of flora in intestinal tracts, promote the absorption of nutrient substances, reduce the content of serum cholesterol, prevent cardiovascular diseases and inhibit the formation of tumor cells. Lactobacillus casei belongs to the genus Lactobacillus, is a gram-positive bacterium, does not produce spores, flagellum and motion, is facultative heterotypic fermented lactose, can survive in a large amount in intestinal tracts after entering animals, can effectively regulate the balance of intestinal flora, promote digestion and absorption, and can promote cell division, generate antibody immunity, enhance immunity and inhibit tumor growth. The bacillus subtilis is one of bacillus, can secrete a plurality of enzyme substances and growth factors when producing spores, has excellent stability, has the characteristics of oxidation resistance, extrusion resistance, high temperature resistance, acid resistance and the like, has strong stress resistance, can be stored for a long time, has strong stability in particles or powder, can smoothly enter large and small intestinal tracts of animals to live and can be bred in a large range. In the invention, lactobacillus plantarum and lactobacillus casei are cultured to obtain bacterial powder A, bacillus subtilis is cultured to obtain bacterial powder B, the bacterial powder A and the bacterial powder B are compounded according to a certain proportion to obtain a compound microorganism metabolite, and broiler and piglet feeding experiments on the obtained product show that the daily gain of broiler chickens and white piglets in examples 3-5 is higher, and the feed-meat ratio of broiler chickens and white piglets is relatively lower. In the process of raising experiments of broiler chickens and white piglets, the effect of each group in the example 5 is the best, the daily gain increase is improved by 17.82% compared with a control group in the broiler raising experiments, and the feed-meat ratio is 1.60; in the raising experiment of the white piglet, the daily gain is improved by 20.89 percent compared with the control group, and the feed conversion ratio is 1.72.

In the invention, the composite microbial feed additive is prepared by specific equipment, in other words, the excellent technical effect can be obtained only after the composite microbial feed additive is prepared by the specific equipment of the invention, specifically, the composite microbial feed additive is mainly realized by the layered arrangement and the coating effect of the specific materials, the bacillus subtilis has stronger stability in particles and powder, and can smoothly and stably enter large and small intestinal tracts after entering the stomach, so that the large-range reversal is realized, and the daily weight gain can be effectively improved and the feed-meat ratio can be reduced by matching the specific proportion of lactobacillus plantarum and lactobacillus casei which are coated inside.

The special equipment of the invention, namely the atomization device in the spray drying device can form micron-sized spray droplets, and the spray drying device comprises: a first fluid nozzle 1 and a second fluid nozzle 2, the first fluid nozzle 1 and the second fluid nozzle 2 being symmetrically arranged about a nozzle 13; a first passage between the first fluid nozzle 1 and the nozzle 13, a second passage between the second fluid nozzle 2 and the nozzle 13; a third fluid nozzle 3 located on and in communication with the first channel, a fourth fluid nozzle 4 located on and in communication with the second channel; the first channel is a columnar structure with the width gradually decreasing from an inlet to the nozzle 13, and the second channel comprises a gradually-decreasing-width gradual section 15 close to the inlet of the second channel and a uniform section 16 with the width unchanged close to one side of the nozzle 13; the fourth fluid nozzle 4 is arranged on the transition section 15 of the second channel, is positioned at the junction between the transition section 15 and the uniform section 16, a triangular control plate 5 is arranged near the junction between the transition section 15 and the uniform section 16, the triangular control plate 5 is connected on the inner wall of one side of the second channel close to the fourth fluid channel 4, the triangular control plate 5 is in a right-angled triangle shape and comprises a tip extending to the transition section 15 and a horizontal end just contacting the inlet end of the uniform section, and is positioned at least a first channel plate 6 and a second channel plate 7 arranged on the hypotenuse section of the triangular control plate 5, the directions of the first channel plate and the second channel plate are parallel to the axial direction of the second channel, guide rails 8 are arranged on two outer sides of the first channel plate and the second channel plate, a driving part 9 capable of driving the first channel plate and the second channel plate to move is arranged on the guide rails 8, and the distance between the first channel plate and the second channel plate can be driven by a control part A triangular plate 10 is fixedly arranged at the central position below the horizontal end through a rotating shaft 11, the triangular plate is triangular, the tip end of the triangular plate faces the horizontal end, and the triangular plate 10 can be driven by the rotating motor 12 to rotate; the end part of the first channel close to the nozzle 13 is in a quadrangular pyramid shape, the end part of the second channel close to the nozzle 13 is in a quadrangular prism shape square outlet 14, the length and the width of the inlets of the first fluid channel 1 and the second fluid channel 2 are the same, the length of the first channel is the same as that of the second channel, the first fluid nozzle and the second fluid nozzle are introduced with compressed gas, the third fluid nozzle and the fourth fluid nozzle are respectively introduced with a bacteria liquid A and a bacteria liquid B, namely, the fourth fluid nozzle 4 of the second channel is introduced with a bacteria liquid B.

The composite microbial feed additive prepared by the special equipment can realize the layered composite structure of the thalli A and the thalli B, namely, the bacteria liquid B is divided into a first layer which comprises most bacteria liquid A and collides with the bacteria liquid A to form fog-shaped particles, and a second layer which coats the fog-shaped particles, the inner layer comprises mixed particles of the bacteria liquid A and the bacteria liquid B, so that the effect is optimal after the bacteria liquid B enters the intestinal tract, the coating layer on the outer layer is the bacteria liquid B which is high in smoothness and stability, the protective property during passing is effectively improved, the bacteria liquid B which is propagated firstly after the intestinal tract, the environment after propagation of the bacteria liquid B is further favorable for propagation of the bacteria liquid A and the bacteria liquid B which are subsequently protected, the bacteria liquid B is propagated first to generate a suitable growth environment for the subsequent bacteria liquid, namely, the composite microbial feed additive is at least positioned on the outer layer and mainly comprises the thalli B, so that the stability of the composite microbial feed additive is improved by the mode, the survival rate of beneficial bacteria is improved, the survival rate of the composite microbial feed additive passing through the stomach is further improved, more importantly, the bacteria liquid B is layered through the tip of the triangular control plate, the direction of the thin layer of the bacteria liquid B can be changed by controlling the distance between the first channel plate and the second channel plate, meanwhile, part of the bacteria liquid is scattered in the thin layer manner, so that most of the bacteria liquid flows in through the first channel plate, the rest of the bacteria liquid enters through the second channel plate 7, the tip of the rotating shaft 11 is positioned between the first channel plate and the second channel plate by adjusting the rotating motor 12, the bacteria liquid B can be layered through the arrangement, the layered arrangement can be maintained until the bacteria liquid enters the nozzle 13 through the uniform section arrangement, and further, most of the bacteria liquid B positioned on the inner side of the nozzle 13 collides with the bacteria liquid A through the thin layer manner to form spray liquid drops with small particle sizes, after the liquid drops are formed by collision, the vertical horse and the lower side are layered, namely, the liquid drops are coated by the bacteria liquid B on the lower side of the triangular plate 10, the thickness and the compactness of the coating layer of the bacteria liquid B can be controlled according to actual requirements by controlling the position between the triangular plate and the first channel plate and the second channel plate, the higher the flow rate of the lower layer is, the higher the compactness and the thickness are, namely, the compound vitamin feed additive which is efficient and is in a desired proportion can be formed to the maximum extent by matching the triangular control plate 5 with the first channel plate and the second channel plate, so that the formed compound microorganism feed additive effectively reduces the damage or death of beneficial strains, finally, not only improves the production efficiency, but also improves the quantity of the beneficial strains, and when the formed spray liquid drops enter a drying chamber, moisture contained in the spray liquid drops is quickly gasified at the entrance of the drying chamber to absorb heat, so as to reduce the temperature in the drying chamber, the invention also provides another important invention point, which effectively reduces the damage or death of beneficial bacteria.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present application have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the application, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A preparation method of a compound microorganism feed additive is characterized in that,

Fungus powder A: 50% of lactobacillus plantarum and 50% of lactobacillus casei;

and (3) fungus powder B: 100% of bacillus subtilis;

the preparation method of the fungus powder A comprises the following steps:

s2, inoculating the seed liquid to a fermentation medium, namely 3.6kg of peptone, 2.4kg of yeast extract, 0.6kg of beef extract, 3.2kg of glucose, 0.06kg of magnesium sulfate, 1.2kg of triammonium citrate, 1.8kg of sodium acetate, 600L of distilled water, sterilizing at 121 ℃ for 15min to obtain fermentation liquid;

s3, after the fermentation is finished, concentrating the fermentation liquor by using a membrane concentrator, and collecting concentrated liquor;

s4, adding 0.2kg of skimmed milk powder, 1.2kg of lactose and 0.1kg of sodium alginate into the concentrated solution, and uniformly stirring to obtain a bacterial fluid A;

2. The method for preparing a compound microbial feed additive according to claim 1, wherein the culture conditions in step S1 are as follows: the culture temperature is 35 ℃ and 2 ℃, and the culture time is 12h-20 h.

3. The method for preparing a compound microbial feed additive according to claim 2, wherein the culture conditions in step S2 are as follows: the culture temperature is 34 +/-1 ℃, and the culture time is 12-16 h.

4. The preparation method of the compound microbial feed additive according to claim 1, wherein the preparation method of the bacterial powder B comprises the following steps:

t1, inoculating bacillus subtilis to LB culture medium, namely 10g of peptone, 5g of yeast extract, 10g of NaCl, 1000ml of distilled water, 20g of agar, sterilizing at 121 ℃ for 20min, and culturing to obtain seed liquid;

t2, inoculating the seed liquid to a fermentation medium, adding 12kg of sucrose, 9kgL kg of peptone, 3kg of yeast extract powder, 0.6kg of magnesium sulfate, 1.2kg of monopotassium phosphate, 1.2kg of dipotassium phosphate and 600L of distilled water, adjusting the pH to 7.0, and sterilizing for 15min by high-pressure steam at 121 ℃;

t3, after the fermentation is finished, concentrating the fermentation liquor by using a membrane concentrator, and collecting the concentrated liquor;

t4, adding 0.15kg of skimmed milk powder, 1.2kg of lactose and 0.15kg of sodium alginate into the concentrated solution, and uniformly stirring to obtain a bacterial fluid B;

and T5, performing spray drying by a vacuum low-temperature dryer, and performing vacuum low-temperature drying to obtain the product.

5. The method for preparing a compound microbial feed additive according to claim 4, wherein the culture conditions in step T1 are as follows: the culture temperature is 37 +/-0.5 ℃, and the culture time is 12-18 h.

6. The method for preparing the compound microbial feed additive according to claim 4, wherein the culture conditions in the step T2 are as follows: the culture temperature is 35 +/-0.5 ℃, and the culture time is 12-18 h.

7. The method for preparing a compound microbial feed additive according to claim 1, wherein the compound microbial feed additive comprises the following components: and compounding the obtained bacterial powder A and bacterial powder B according to the mass ratio of 1:1-1.2 to obtain the compound microbial metabolite.

8. The special production equipment for the compound microorganism feed additive as claimed in any one of claims 1 to 7, wherein the atomization device in the spray drying device is capable of forming micron-sized spray droplets, and the spray drying device comprises:

a first fluid nozzle (1) and a second fluid nozzle (2), the first fluid nozzle (1) and the second fluid nozzle (2) being symmetrically arranged with respect to the nozzle (13);

a first passage between the first fluid nozzle (1) and the nozzle (13), a second passage between the second fluid nozzle (2) and the nozzle (13);

a third fluid nozzle (3) located on the first channel and in communication with the first channel, a fourth fluid nozzle (4) located on the second channel and in communication with the second channel;

The first channel is a columnar structure with gradually reduced width from an inlet to the nozzle (13), and the second channel comprises a gradually-reduced width section (15) close to the inlet of the second channel and a uniform section (16) with constant width close to one side of the nozzle (13);

fourth fluid nozzle (4) set up in on gradual change section (15) of second passageway, be located gradual change section (15) with near junction of even section (16) is provided with triangle control board (5), triangle control board (5) are connected the second passageway is close to on the inner wall of fourth fluid passageway (4) one side, triangle control board (5) are the right triangle shape and including stretching to the most advanced of gradual change section (15) and just contact the horizontal end of the entry end of even section is located the hypotenuse section of triangle control board (5) is provided with first passageway board (6) and second passageway board (7) at least, the direction of first passageway board and second passageway board with the axial direction of second passageway is parallel, the two outsides of first passageway board and second passageway board are provided with guide rail (8), be provided with on guide rail (8) and drive the drive division that first passageway board and second passageway board removed The distance between the first channel plate and the second channel plate can be controlled by driving the driving part (9) through a control part, a triangular plate (10) is fixedly arranged at the center position below the horizontal end through a rotating shaft (11), the triangular plate is triangular, the tip end of the triangular plate faces the horizontal end, and the triangular plate (10) can be driven by a rotating motor (12) to rotate;

The end part of the first channel close to the nozzle (13) is in a quadrangular pyramid shape, the end part of the second channel close to the nozzle (13) is a square outlet (14) in a quadrangular prism shape, the length and the width of the inlet of the first fluid channel (1) and the inlet of the second fluid channel (2) are the same, and the length of the first channel and the length of the second channel are the same.

9. The special equipment for compounding the microbial feed additive according to claim 8, wherein the first fluid nozzle and the second fluid nozzle are filled with compressed gas, and the third fluid nozzle and the fourth fluid nozzle are filled with the microbial solution A and the microbial solution B respectively.

10. The special equipment for the compound microorganism feed additive as claimed in claim 9, wherein the compressed gas is one of air, carbon dioxide, nitrogen or argon.