CN114747656A - Resistance-reducing probiotic fermented feed, preparation method and preparation device thereof - Google Patents
Resistance-reducing probiotic fermented feed, preparation method and preparation device thereof Download PDFInfo
- Publication number
- CN114747656A CN114747656A CN202210246719.8A CN202210246719A CN114747656A CN 114747656 A CN114747656 A CN 114747656A CN 202210246719 A CN202210246719 A CN 202210246719A CN 114747656 A CN114747656 A CN 114747656A
- Authority
- CN
- China
- Prior art keywords
- feed
- preparation
- culture
- fermented feed
- tank
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000006041 probiotic Substances 0.000 title claims abstract description 51
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 51
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 48
- 238000002360 preparation method Methods 0.000 title claims description 48
- 238000000855 fermentation Methods 0.000 claims abstract description 59
- 230000004151 fermentation Effects 0.000 claims abstract description 59
- 239000007788 liquid Substances 0.000 claims abstract description 35
- 239000000463 material Substances 0.000 claims abstract description 25
- 239000002994 raw material Substances 0.000 claims abstract description 22
- 238000001704 evaporation Methods 0.000 claims abstract description 17
- 230000008020 evaporation Effects 0.000 claims abstract description 15
- 241000894006 Bacteria Species 0.000 claims abstract description 8
- 239000001963 growth medium Substances 0.000 claims description 21
- 230000000813 microbial effect Effects 0.000 claims description 16
- 238000002156 mixing Methods 0.000 claims description 14
- 230000002457 bidirectional effect Effects 0.000 claims description 12
- 241000194032 Enterococcus faecalis Species 0.000 claims description 11
- 244000199866 Lactobacillus casei Species 0.000 claims description 11
- 235000013958 Lactobacillus casei Nutrition 0.000 claims description 11
- 241000186673 Lactobacillus delbrueckii Species 0.000 claims description 11
- 229940032049 enterococcus faecalis Drugs 0.000 claims description 11
- 229940017800 lactobacillus casei Drugs 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 238000009630 liquid culture Methods 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 9
- 239000010902 straw Substances 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 230000003321 amplification Effects 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 6
- 240000006162 Chenopodium quinoa Species 0.000 claims description 4
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 claims description 4
- 235000019779 Rapeseed Meal Nutrition 0.000 claims description 4
- 240000008042 Zea mays Species 0.000 claims description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 4
- 235000005822 corn Nutrition 0.000 claims description 4
- 238000009629 microbiological culture Methods 0.000 claims description 4
- 239000004456 rapeseed meal Substances 0.000 claims description 4
- 230000008901 benefit Effects 0.000 claims description 2
- 238000005469 granulation Methods 0.000 claims description 2
- 230000003179 granulation Effects 0.000 claims description 2
- 238000000227 grinding Methods 0.000 claims description 2
- 239000002054 inoculum Substances 0.000 claims description 2
- 241000883990 Flabellum Species 0.000 claims 2
- 241000486679 Antitype Species 0.000 claims 1
- 240000004658 Medicago sativa Species 0.000 claims 1
- 239000004615 ingredient Substances 0.000 claims 1
- 210000002966 serum Anatomy 0.000 abstract description 9
- 102100036475 Alanine aminotransferase 1 Human genes 0.000 abstract description 4
- 108010082126 Alanine transaminase Proteins 0.000 abstract description 4
- 108010088751 Albumins Proteins 0.000 abstract description 4
- 102000009027 Albumins Human genes 0.000 abstract description 4
- 102000002260 Alkaline Phosphatase Human genes 0.000 abstract description 4
- 108020004774 Alkaline Phosphatase Proteins 0.000 abstract description 4
- 108010044091 Globulins Proteins 0.000 abstract description 4
- 102000006395 Globulins Human genes 0.000 abstract description 4
- 108090000340 Transaminases Proteins 0.000 abstract description 4
- 102000004169 proteins and genes Human genes 0.000 abstract description 4
- 108090000623 proteins and genes Proteins 0.000 abstract description 4
- 102000014898 transaminase activity proteins Human genes 0.000 abstract description 4
- 241000283903 Ovis aries Species 0.000 description 21
- 235000019687 Lamb Nutrition 0.000 description 9
- 238000007599 discharging Methods 0.000 description 8
- 241001465754 Metazoa Species 0.000 description 6
- 241000186660 Lactobacillus Species 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 229940039696 lactobacillus Drugs 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 241000219823 Medicago Species 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 235000018102 proteins Nutrition 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000186000 Bifidobacterium Species 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 102000010911 Enzyme Precursors Human genes 0.000 description 2
- 108010062466 Enzyme Precursors Proteins 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 235000012000 cholesterol Nutrition 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000004584 weight gain Effects 0.000 description 2
- 235000019786 weight gain Nutrition 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000283898 Ovis Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- PNNCWTXUWKENPE-UHFFFAOYSA-N [N].NC(N)=O Chemical compound [N].NC(N)=O PNNCWTXUWKENPE-UHFFFAOYSA-N 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- YXVFQADLFFNVDS-UHFFFAOYSA-N diammonium citrate Chemical compound [NH4+].[NH4+].[O-]C(=O)CC(O)(C(=O)O)CC([O-])=O YXVFQADLFFNVDS-UHFFFAOYSA-N 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000036449 good health Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000012165 high-throughput sequencing Methods 0.000 description 1
- 235000020256 human milk Nutrition 0.000 description 1
- 210000004251 human milk Anatomy 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 1
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108010009004 proteose-peptone Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01F—PROCESSING OF HARVESTED PRODUCE; HAY OR STRAW PRESSES; DEVICES FOR STORING AGRICULTURAL OR HORTICULTURAL PRODUCE
- A01F29/00—Cutting apparatus specially adapted for cutting hay, straw or the like
- A01F29/02—Cutting apparatus specially adapted for cutting hay, straw or the like having rotating knives with their cutting edges in a plane perpendicular to their rotational axis
- A01F29/025—Cutting apparatus specially adapted for cutting hay, straw or the like having rotating knives with their cutting edges in a plane perpendicular to their rotational axis with feeding direction parallel to axis
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01F—PROCESSING OF HARVESTED PRODUCE; HAY OR STRAW PRESSES; DEVICES FOR STORING AGRICULTURAL OR HORTICULTURAL PRODUCE
- A01F29/00—Cutting apparatus specially adapted for cutting hay, straw or the like
- A01F29/09—Details
- A01F29/12—Discharge means
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23N—MACHINES OR APPARATUS FOR TREATING HARVESTED FRUIT, VEGETABLES OR FLOWER BULBS IN BULK, NOT OTHERWISE PROVIDED FOR; PEELING VEGETABLES OR FRUIT IN BULK; APPARATUS FOR PREPARING ANIMAL FEEDING- STUFFS
- A23N17/00—Apparatus specially adapted for preparing animal feeding-stuffs
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C13/00—Disintegrating by mills having rotary beater elements ; Hammer mills
- B02C13/14—Disintegrating by mills having rotary beater elements ; Hammer mills with vertical rotor shaft, e.g. combined with sifting devices
- B02C13/18—Disintegrating by mills having rotary beater elements ; Hammer mills with vertical rotor shaft, e.g. combined with sifting devices with beaters rigidly connected to the rotor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/137—Delbrueckii
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Animal Husbandry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Environmental Sciences (AREA)
- Mycology (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Virology (AREA)
- Birds (AREA)
- Tropical Medicine & Parasitology (AREA)
- Clinical Laboratory Science (AREA)
- Mechanical Engineering (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses an anti-attenuation probiotic fermented feed, which relates to the technical field of feed processing and comprises a strain fermentation tank, a feed fermentation tank, a material crusher, a mixer, an evaporation tank and an oven, wherein a fermented bacteria liquid discharge pipe is arranged at the bottom of the strain fermentation tank and is connected to one side of the top of the feed fermentation tank, a flowmeter is arranged on the fermented bacteria liquid discharge pipe, a discharge pipe of the feed fermentation tank is connected to a raw material liquid inlet of the evaporation tank, and the material crusher and the mixer are arranged beside the feed fermentation tank. The feed can improve the contents of albumin, globulin and total protein in blood serum of the cubs, and the contents of glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and alkaline phosphatase in the blood serum are lower than those of the cubs fed by the commercially-purchased feed.
Description
Technical Field
The invention relates to the technical field of feed processing, in particular to an anti-attenuation probiotic fermented feed, a preparation method and a preparation device thereof.
Background
Young animals have a weak immune system, and in farms that raise animals in large numbers, the animals start to feed with the feed several months later, and when the young animals have a weak ability to digest the feed and are unable or slow to digest it, the young animals absorb and metabolize slowly, which affects development. In China, the feeding of the piglets needs to treat the feed for eating, so that the feed is easy to digest and absorb, and can supplement enough nutrition, particularly in the conversion process of milk feeding and feed feeding, the feed is easy to absorb like breast milk, and the piglets cannot generate larger resistance. Therefore, it is important that reduced resistance feeds be used to feed young animals.
Disclosure of Invention
The present invention is directed to solving, at least in part, one of the technical problems in the related art. Therefore, an object of the present invention is to provide an anti-reducing probiotic fermented feed, a preparation method and a preparation device thereof, so as to solve the problems in the background art.
The utility model provides a preparation facilities of subtracting resistant type benefit fermented feed which characterized in that: comprises that
The fermentation tank of bacterial for ferment the bacterial, still include:
feed fermentation tank for ferment the fodder, still include:
The material crusher is used for crushing the feed raw materials; further comprising:
the mixer is used for mixing the feed and the strains; further comprising:
the evaporation tank is used for providing high-temperature conditions for the fermentation process of the feed; further comprising:
the drying oven is used for drying the generated feed;
be equipped with the zymophyte liquid calandria on the bacterial fermentation tank bottom, the zymophyte liquid calandria inserts the one side at feed fermentation tank top, be equipped with the flowmeter on the zymophyte liquid calandria, feed fermentation tank's row expects on the pipe inserts the raw materials liquid entry of evaporating pot, material crusher and blendor all set up by feed fermentation tank.
In some embodiments of the invention, the feed fermentation tank comprises a tank body, a base, a supporting and adjusting structure, a feed opening, a top inlet and an internal smashing structure, wherein the tank body is arranged on the base through the supporting and adjusting structure, the supporting and adjusting structure is used for controlling feeding, the top inlet is arranged at the top of the tank body, the feed opening is arranged at the bottom of the tank body, and the internal smashing structure is arranged in the tank body and is used for further smashing feed raw materials.
In some embodiments of the present invention, the supporting and adjusting structure includes a sliding panel, a bottom supporting seat and a power component, the tank is fixed on the bottom supporting seat, a sliding groove is formed in the bottom supporting seat, the sliding panel is slidably disposed in the sliding groove, and the power component is disposed on a side of the sliding panel.
In some embodiments of the present invention, the power parts include a driving rack gear, a driving gear, a moving support rod, a moving slide plate, a buffer spring, and an adjustment support plate, the tank body is fixed on the bottom supporting seat, a sliding groove is arranged on the bottom supporting seat, a sliding panel is arranged in the sliding groove in a sliding manner, a movable sliding rod is fixed at the top of the sliding panel, a movable sliding plate is fixed at the end part of the movable sliding rod and is connected with the bottom supporting seat in a sliding way, a buffer spring is fixed on the top of the movable sliding plate, the other end of the buffer spring is fixed on the top of the bottom supporting seat, an adjusting support plate is fixed on the side edge of the sliding panel, a driving rack is uniformly arranged on the adjusting support plate, the inside drive gear that is provided with through the power spare of bottom sprag seat just drive gear and drive rack meshing connect.
In some embodiments of the invention, the internal crushing structure comprises a blanking control part, crushing blades, a driving rotating shaft, a crushing support seat and a bidirectional motor, the blanking control part is arranged at the bottom of the tank body, the bidirectional motor is fixed at the middle position in the tank body through a transverse support, the driving rotating shaft and the blanking control part are respectively and rotatably arranged at two sides of the bidirectional motor, the crushing support seat is fixed on the driving rotating shaft, and the crushing blades are uniformly fixed on the crushing support seat.
In some embodiments of the present invention, the blanking control member includes a bottom blanking port, a rotating plate, a chassis, internal teeth, a bottom gear, a rotating chassis and a connecting rod, a rotating disc is fixed at the bottom of the tank, the rotating disc is provided with the bottom blanking port, the bottom of the tank is provided with the blanking port, the blanking port is communicated with the bottom blanking port, two sides of the bidirectional motor are respectively and rotatably provided with a driving rotating shaft and a bottom rotating shaft, the bottom rotating shaft is arranged at an output end of the bidirectional motor through an automatic clamping member, the end of the bottom rotating shaft is fixed with the bottom gear, the chassis is rotatably provided with the rotating plate, the rotating plate is rotatably arranged on the rotating chassis in a fan shape through the connecting rod, the internal teeth are arranged inside the rotating chassis, and the internal teeth are meshed with the bottom gear.
An anti-reducing probiotic fermented feed is prepared by mixing a microbial agent prepared by microbial culture with a fermented feed and fermenting;
the microbial culture preparation comprises the steps of respectively inoculating lactobacillus casei DF-3, lactobacillus delbrueckii JY-11 and enterococcus faecalis GY-9 to an MRS solid slant culture medium to be cultured to obtain a seed slant; respectively inoculating seed slopes of lactobacillus casei DF-3, lactobacillus delbrueckii JY-11 and enterococcus faecalis GY-9 in an MRS liquid culture medium to obtain respective liquid seed liquid; performing liquid expanded culture of strains, and respectively inoculating Lactobacillus casei DF-3, Lactobacillus delbrueckii JY-11 and enterococcus faecalis GY-9 into a 50L fermentation tank for culture; the volume ratio of the microbial agent for enlarged culture is 1: 1: 1, and mixing uniformly to obtain the novel resistance-reducing probiotic preparation.
A preparation method of an anti-reducing probiotic fermented feed comprises the following specific steps:
s1, preparation of a microbial preparation: 1. respectively inoculating lactobacillus casei DF-3, lactobacillus delbrueckii JY-11 and enterococcus faecalis GY-9 to an MRS solid slant culture medium to culture to obtain a seed slant; 2. respectively inoculating the seed slopes into an MRS liquid culture medium to obtain respective liquid seed solutions; 3. respectively inoculating the liquid seed solutions into 50L fermentation tanks for culture; 4. and (3) mixing the microbial agent subjected to amplification culture in a volume ratio of 1: 1: 1, uniformly mixing to obtain a novel antibiotic-reducing probiotic preparation;
s2, preparation of probiotic fermented feed: putting alfalfa, corn straw and quinoa straw into a grinder according to a certain proportion, grinding the mixture to a length of 2.0-2.5mm, and then adding and mixing rapeseed meal according to a certain proportion to obtain a feed fine material;
s3, fermenting the fermented feed: adding a microbial preparation into the probiotic fermented feed according to 1-2% of the total weight of the probiotic fermented feed, adding water, adjusting the water content in the feed to 65-75%, placing the fermented material in a closed fermentation tank for fermentation for 30-40d, wherein the fermentation temperature is 40-45 ℃;
s4, granulating: putting the fermented feed in the step S3 into an evaporation tank for evaporation to ensure that the water content is lower than 30%, taking out the evaporated feed, putting the evaporated feed into an oven for drying to ensure that the water content is lower than 10%, and finally, transferring the evaporated feed into a granulator for granulation to prepare granulated feed.
In other embodiments of the invention, the slant culture medium culture conditions are: culturing in a constant temperature incubator at 37 deg.C for 48-52 h; the culture conditions of the MRS liquid culture medium are as follows: culturing in a shaking incubator at 37 deg.C and 200r/min for 48-52 h; the culture conditions of the amplification culture are as follows: inoculating into 50L fermentation tank, wherein the culture medium is MRS liquid culture medium with an inoculum size of 10%, culturing at 37 deg.C and 200r/min for 48-52h with effective viable bacteria number of 5 × 108CFU/mL or above; the temperature of the evaporation zone in the evaporation tank is 55-65 ℃.
According to the invention, the feed can improve the contents of albumin, globulin and total protein in blood serum of the cubs, and the contents of glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and alkaline phosphatase in the blood serum are lower than those of the cubs fed by the commercially purchased feed; by adopting the supporting and adjusting structure, the height of the tank body can be adjusted, so that waste caused by splashing during blanking can be avoided, and the bottom collecting box can be conveniently pulled out; this application adopts corresponding smashing just to combine together with unloading control unit for can utilize two way motor's action, also can further smash at the in-process of unloading, thereby can carry out the unloading at the pivoted in-process, and the material can fully smash under the effect of gravity, if the effect of smashing is not good, also can utilize automatic joint spare control chassis not to rotate, shelters from the bottom feed opening.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
fig. 1 is a schematic structural layer diagram of a resistance-reducing probiotic fermented feed preparation device provided by the invention.
FIG. 2 is a comparative experimental diagram of the preparation of a resistance-reducing probiotic fermented feed.
Fig. 3 is a schematic diagram of a material fermentation tank of the resistance-reducing probiotic fermented feed preparation device provided by the invention.
Fig. 4 is a schematic view of a support and adjustment structure of an anti-reduction type probiotic fermented feed preparation device provided by the invention.
Fig. 5 is a schematic view of a bottom feed opening of the resistance-reducing probiotic fermented feed preparation device provided by the invention.
FIG. 6 is a schematic diagram of the internal crushing of a resistance-reducing probiotic fermented feed preparation device provided by the invention.
In the figure: 1. a strain fermentation tank; 2. a flow meter; 3. a feed fermentation tank; 4. an evaporator tank; 5. a material crusher; 6. a mixer; 7. a top access port; 8. a tank body; 9. a bottom support seat; 10. a sliding panel; 11. a base; 12. a feeding port; 13. a drive rack; 14. a drive gear; 15. moving the support rod; 16. moving the sliding plate; 17. a buffer spring; 18. adjusting the support plate; 19. a bottom feed opening; 20. breaking the supporting seat; 21. a bi-directional motor; 22-bottom gear; 23. rotating the chassis; 24. a connecting rod; 25. a rotating plate; 26. a chassis; 27. an inner tooth; 28. breaking the fan blades; 29. breaking the rotating shaft.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Example 1: please refer to FIGS. 1-6
Preparation of resistance-reducing probiotic preparation
(1) Preparing microorganism slant seeds: inoculating lactobacillus casei DF-3, lactobacillus delbrueckii JY-11 and enterococcus faecalis GY-9 to an MRS solid slant culture medium, and culturing at the constant temperature of 37 ℃ for 48-52h to obtain the seed slant.
The MRS solid slant culture medium comprises: 10g of casein peptone, 10g of beef extract, 5g of yeast powder, 5g of glucose, 5g of sodium acetate, 2g of diammonium citrate, 801g of Tween, 2g of dipotassium phosphate, 0.2g of magnesium sulfate heptahydrate, 0.05g of manganese sulfate, 20g of calcium carbonate, 20g of agar, 1000mL of distilled water and 6.8 of pH. Sterilizing with high pressure steam at 121 deg.C for 20 min.
(2) Culturing liquid seeds of the microorganisms: respectively inoculating lactobacillus casei DF-3, lactobacillus delbrueckii JY-11 and enterococcus faecalis GY-9 to an MRS liquid culture medium, and performing shake culture at 37 ℃ and 200r/min for 48-52h to obtain liquid seeds.
(3) Liquid amplification culture of the strain: respectively inoculating Lactobacillus casei DF-3, Lactobacillus delbrueckii JY-11 and enterococcus faecalis GY-9 into a 50L fermentation tank, wherein the culture medium is an MRS liquid culture medium, the inoculation amount is 10%, culturing is carried out for 48-52h at 37 ℃ and the rotating speed of 200r/min, and when the number of effective viable bacteria in the 3 lactobacillus liquid is 5 multiplied by 10 8Stopping fermentation when the concentration is above CFU/mL.
(4) Preparation of microbial preparation: and (4) respectively carrying out amplification culture on the microbial agents in the step (3) according to the volume ratio of 1: 1: 1, mixing uniformly to obtain the novel antibiotic-reducing probiotic preparation.
Example 2 please refer to FIGS. 1-6
Preparation of resistance-reducing probiotic fermented feed
The fermented feed comprises the following raw materials: 40-50% of alfalfa, 20-35% of fresh corn straw, 20-35% of quinoa straw and 5-10% of rapeseed meal.
(1) Putting the alfalfa into a pulverizer to be pulverized; placing the corn straw and the quinoa straw into a crusher together, and crushing to a length of about 2.0-2.5 mm; adding the rapeseed meal into the crushed materials according to the weight proportion, and mixing in a mixer to obtain a feed refined material;
(2) adding the compound microbial preparation (the resistance-reducing probiotic preparation) prepared in the example 1 according to the proportion of 1 percent of the total weight of the refined materials, uniformly mixing, and adding water to adjust the water content in the feed to 65-75 percent;
(3) and (3) placing the fermented material obtained in the step (2) in a closed environment (a feed fermentation tank) for fermentation for 30-40d to obtain the novel resistance-reducing probiotic fermented feed.
Test 1
24 lambs with similar weight and good health condition for about 3 months are selected for the test, and are randomly divided into 3 groups, and each group comprises 8 lambs, namely a test group 1, a test group 2 and a control group. The feeding conditions of all groups of lambs are the same, and the lambs are fed along with the ewes in a barn way and fed regularly. During supplementary feeding, the lambs and the ewes are separated and fed with supplementary feeds respectively, the test group 1 is fed with the resistance-reducing probiotic fermented feed, the test group 2 is fed with commercial feed (clorson, jiangming (china) science and technology limited), and the control group is not fed with supplementary feeds. The lambs adopt supplementary feeding barriers, and only the lambs can freely come in and go out. In the pre-test period, the health of the lambs is checked, the lambs are subjected to parasite expelling and epidemic prevention treatment, numbered and fixed in a sheep house, and the lambs are transferred to the normal test period after being adapted to the environment. The pre-test period is 7 days, and the test period is 28 days. Feeding at 06:00 and 18:00 each day, and feeding freely and drinking freely. The test lambs were weighed on days 0, 7, 14 and 28 of the test, respectively.
TABLE 1 Effect of supplementary feeding of probiotics on lamb growth Performance
| Test group 1 | |
Control group | |
| weight/Kg of lambs at the beginning of the test | 4.22±0.43 | 4.81±0.32 | 4.57±0.48 |
| weight/Kg of lamb after test | 16.97±2.57 | 17.04±2.48 | 16.33±2.65 |
| Weight gain/Kg in test | 12.75±1.34 | 12.23±2.04 | 11.76±2.13 |
| Average daily gain/Kg | 0.46±0.03 | 0.44±0.05 | 0.42±0.03 |
| Material to weight ratio | 0.29 | 0.37 | 0.43 |
And (3) test results: from table 1, it can be seen that the addition of probiotics contributes to the improvement of the growth performance of lambs. The weight gain, average daily gain and feed-weight ratio of the resistance-reducing probiotic fermented feed to the lambs are higher than those of a commodity group and a control group, and the novel resistance-reducing probiotic fermented feed is beneficial to improving the growth performance of the lambs.
Test example 2
The lamb feeding management is the same as above. After the test is finished, 10mL of jugular venous blood of each group of lambs is collected, serum is separated after standing, the lambs are stored at the temperature of minus 20 ℃, and relevant blood biochemical indexes are determined.
TABLE 2 Effect of supplementary feeding of probiotics on Biochemical indicators of lamb blood
| Test group 1 | |
Control group | |
| Total protein g/L | 46.69±2.13 | 43.76±2.26 | 43.18±2.47 |
| Albumin g/L | 33.48±2.42 | 32.28±2.44 | 30.19±2.39 |
| Globulin g/L | 13.48±1.85 | 13.04±1.48 | 11.33±1.75 |
| Glutamic-pyruvic transaminase U/L | 8.47±3.48 | 8.62±3.35 | 9.84±2.68 |
| Glutamic-oxalacetic transaminase U/L | 75.39±11.46 | 80.45±10.84 | 83.93±11.54 |
| Alkaline phosphatase U/L | 514.48±85.38 | 507.33±95.38 | 615.37±98.67 |
| Total Cholesterol mmol/L | 2.32±0.47 | 2.11±0.31 | 2.08±0.43 |
| Nitrogen mmol/L of urea | 7.03±1.38 | 7.68±2.04 | 7.48±2.42 |
| Glucose mmol/L | 5.33±1.31 | 4.95±1.48 | 5.03±1.04 |
The results show that: the contents of albumin, globulin and total protein in the lamb serum of the probiotic group are higher than those of the control group; the contents of glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and alkaline phosphatase in the blood serum of the lambs in the probiotic group are lower than those in the control group; the total cholesterol content of the probiotic group is higher than that of the control group; the resistance-reducing probiotic fermented feed is better than a commercial control. The content of the serum urea nitrogen of the lambs is the lowest in the test group 1; the highest in the serum glucose test group 1 indicates that the resistance-reducing probiotic fermented feed is beneficial to various metabolism and physiological activities in lambs.
Test example 3
The lamb breeding management is the same as above. After the test is finished, fresh excrement samples of all groups of lambs are collected and stored at the temperature of 80 ℃ below zero, and the change of excrement microbial flora is determined through high-throughput sequencing.
The results show that: compared with a control group, the probiotic group changes the lamb feces microbial flora mainly to increase bifidobacterium, increase the lactobacillus number and reduce escherichia coli; the microbial numbers of bifidobacterium and lactobacillus in the lamb feces of the resistance-reducing probiotic fermented feed group are obviously increased, which shows that the fermented feed is beneficial to the increase of the number of beneficial microbes of lamb intestinal lactobacillus and the reduction of the number of disease microbes such as escherichia coli and the like.
Referring to the feed preparation device, referring to fig. 1, the device comprises a strain fermentation tank 1, a feed fermentation tank 3, a material crusher 5, a mixer 6, an evaporation tank 4 and an oven, wherein the strain fermentation tank 1 is a device for fermenting strains required by the feed, a fermented bacteria liquid discharging pipe is arranged at the bottom of the strain fermentation tank 1 and is connected to one side of the top of the feed fermentation tank 3, so that the fermented bacteria liquid reaches the inside of the feed fermentation tank 3 through the fermented bacteria liquid discharging pipe, wherein, a flowmeter 2 is arranged on the zymophyte liquid calandria, wherein, the flowmeter 2 is used for detecting the flow of the entering zymophyte liquid, a discharge pipe of the feed fermentation tank 3 is connected to a raw material liquid inlet of the evaporation tank 4, a material crusher 5 and a mixer 6 are both arranged beside the feed fermentation tank 3, used for filling the raw materials obtained by the material crusher 5 into the feed fermentation tank 3 after being crushed.
As a further embodiment of this application, please refer to fig. 1 and fig. 3, wherein feed fermentation tank 3 includes a tank body 8, a base 11, a support adjusting structure, a feed opening 12, a top inlet 7 and an internal smashing structure, at first, the tank body 8 is arranged on the base 11 through the support adjusting structure, the longitudinal height of the tank body 8 can be adjusted by the support adjusting structure, the top of the tank body 8 is provided with the top inlet 7, the top inlet 7 is used for entering the required raw material, a feed opening 12 is arranged at the bottom of the tank body 8, the feed of the finished product is realized by the feed opening 12, the internal smashing structure is arranged inside the tank body 8, and the raw material is smashed by the internal smashing structure. In this embodiment, at first get into the raw materials from top inlet port 7, the raw materials is smashed the structure through inside in jar 8 insides, smashes the back, utilizes the adjustment of the vertical height that supports the adjustment structure and realize jar 8 of body, can be convenient for carry out the unloading, utilizes the jar body 8 of longitudinal movement, can shift out the collecting vessel that will be convenient for load the finished product fodder from feed opening 12.
As a further embodiment of the present application, please refer to fig. 1, fig. 3 and fig. 4, wherein in order to realize the adjustment of the overall height of the tank 8, the support adjusting structure comprises a sliding panel 10, a bottom supporting seat 9, a driving rack 13, a driving gear 14, a movable supporting rod 15, a movable sliding plate 16, a buffer spring 17 and an adjusting supporting plate 18, the tank 8 is firstly fixed on the bottom supporting seat 9, in order to realize the longitudinal height adjustment of the tank 8, a sliding groove is opened on the bottom supporting seat 9, the sliding panel 10 is slidably arranged in the sliding groove, the height of the tank 8 is controlled by the length extending between the sliding panel 10 and the bottom supporting seat 9, wherein the movable sliding rod 15 is fixed on the top of the sliding panel 10, the movable sliding rod 15 is fixed on the end of the movable sliding plate 16, and the movable sliding plate 16 is slidably connected with the bottom supporting seat 9, namely, when the movable sliding plate 16 moves longitudinally, the buffer spring 17 is fixed at the top of the movable sliding plate 16, and the other end of the buffer spring 17 is fixed at the top of the bottom supporting seat 9, so as to buffer the position of the sliding panel 10, when the power element fails, the buffer tank body 8 can be buffered under the action of the buffer spring 17, the adjusting supporting plate 18 is fixed at the side edge of the sliding panel 10, the driving racks 13 are uniformly arranged on the adjusting supporting plate 18, the driving gear 14 is arranged in the bottom supporting seat 9 through the power element, and the driving gear 14 is meshed and connected with the driving racks 13, namely, the driving gear 14 rotates under the action of the power element, so that the positions of the driving gear 14 and the driving racks 13 move relatively, and the adjustment of the longitudinal height of the bottom supporting seat 9 can be realized;
In order to control the crushing of the raw material and the controlled discharging of the crushed raw material, in this embodiment, referring to fig. 1, fig. 3, fig. 5 and fig. 6, the internal crushing structure comprises a bottom discharge port 19, a rotating plate 25, a base plate 26, internal teeth 27, crushing blades 28, a driving rotating shaft 29, a crushing support 20, a bidirectional motor 21, a bottom gear 22, a rotating base plate 23 and a connecting rod 24, i.e. firstly, a rotating disc 26 is fixed at the bottom of the tank 8, the rotating disc 26 is provided with a bottom discharge port 19 for realizing the discharging, wherein the bottom of the tank 8 is provided with a discharge port 12, the discharge port 12 is communicated with the bottom discharge port 19, the discharging can be performed when the bottom discharge port 19 is not shielded, wherein the bidirectional motor 21 is fixed at the middle position inside the tank 8 through a transverse support, and two sides of the bidirectional motor 21 are respectively and rotatably provided with the driving rotating shaft 29 and the bottom rotating shaft, the bottom rotating shaft is arranged at the output end of the bidirectional motor 21 through an automatic clamping piece, namely whether the bidirectional motor 21 drives the bottom rotating shaft to rotate or not is realized through the automatic clamping piece, wherein crushing fan blades 28 are uniformly arranged on a driving rotating shaft 29, the raw materials are crushed by the crushing fan blades 28, a bottom gear 22 is fixed at the end part of the bottom rotating shaft, a rotating plate 25 is rotatably arranged on a chassis 26, the rotating plate 25 is fan-shaped and is rotatably arranged on a rotating chassis 23 through a connecting rod 24, internal teeth 27 are arranged inside the rotating chassis 23, the internal teeth 27 are meshed and connected with the bottom gear 22, namely, when the bottom gear 22 rotates, the internal teeth 27 can be driven to rotate, so that the rotating plate 25 can be driven to rotate, the rotating plate 25 can be separated from the bottom feed opening 19, so that the feeding is convenient, and by utilizing the rotating chassis 23 and the rotating plate 25, the bottom feed opening 19 can be placed on the rotating chassis 23, so as to facilitate the blanking, a hopper-shaped shield may be provided on the base plate 26, and the output end of the hopper-shaped shield is provided on the bottom blanking opening 19, in order to further facilitate the blanking.
The working raw materials of the device are as follows: the strain fermentation tank 1 is a device for fermenting strains required by feed, a zymogen liquid discharging pipe is arranged at the bottom of the strain fermentation tank 1 and is connected to one side of the top of the feed fermentation tank 3, so that the fermented liquid reaches the inside of the feed fermentation tank 3 through the zymogen liquid discharging pipe, a flow meter 2 is used for detecting the flow of the entered liquid, a discharging pipe of the feed fermentation tank 3 is connected to a raw material liquid inlet of an evaporation tank 4, a material crusher 5 and a mixer 6 are arranged beside the feed fermentation tank 3 and are used for crushing the raw material obtained by the material crusher 5 and then filling the crushed raw material into the feed fermentation tank 3, when the raw material is crushed, the raw material enters from a top inlet 7, the raw material is crushed through an internal crushing structure in the tank 8, after the crushing is finished, the longitudinal height of the tank 8 is adjusted by using a supporting and adjusting structure, lift jar body 8 high, place the collecting tank that will load finished product fodder in jar body 8 bottom, utilize vertical height to reduce the collecting tank afterwards for the collecting tank hugs closely on jar body 8, avoids producing and splashes, when the collecting tank is filled, lifts jar body 8 high, is convenient for pull out the collecting tank, and the crushing function of this application with 3 integrated material rubbing crusher 5 of fodder fermentation cylinder simultaneously can save space.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (9)
1. The utility model provides a subtract preparation facilities of anti type benefit fermented feed which characterized in that: comprises that
The strain fermentation tank (1) is used for fermenting strains and further comprises:
feed fermentation cylinder (3) for ferment the fodder, still include:
the material crusher (5) is used for crushing the feed raw materials; further comprising:
the mixer (6) is used for mixing the feed and the strains; further comprising:
the evaporation tank (4) is used for providing high-temperature conditions for the fermentation process of the feed; further comprising:
the drying oven is used for drying the generated feed;
be equipped with zymophyte liquid calandria on strain fermentation cylinder (1) bottom, the zymophyte liquid calandria inserts the one side at feed fermentation cylinder (3) top, be equipped with flowmeter (2) on the zymophyte liquid calandria, the row of material pipe of feed fermentation cylinder (3) inserts on the feed liquor entry of evaporating pot (4), material crusher (5) and blendor (6) all set up by feed fermentation cylinder (3).
2. The apparatus for preparing a reduced resistance probiotic fermented feed according to claim 1, characterized in that: feed fermentation cylinder (3) are including a jar body (8), base (11), support regulation structure, feed opening (12), top inlet port (7) and inside smash the structure, it is provided with a jar body (8) to adjust the structure through supporting on base (11), support and adjust the structure and be used for controlling the unloading, jar body (8) top is provided with top inlet port (7), jar body (8) bottom is provided with feed opening (12), jar body (8) inside is provided with inside and smashes the structure for carry out further smashing to feed ingredient.
3. The apparatus for preparing a reduced resistance probiotic fermented feed according to claim 1, characterized in that: the supporting and adjusting structure comprises a sliding panel (10), a bottom supporting seat (9) and a power component, the tank body (8) is fixed on the bottom supporting seat (9), a sliding groove is formed in the bottom supporting seat (9), the sliding panel (10) is arranged in the sliding groove in a sliding mode, and the power component is arranged on the side edge of the sliding panel (10).
4. The apparatus for preparing a reduced resistance probiotic fermented feed according to claim 3, characterized in that: the power component comprises a driving rack (13), a driving gear (14), a movable supporting rod (15), a movable sliding plate (16), a buffer spring (17) and an adjusting supporting plate (18), the tank body (8) is fixed on the bottom supporting seat (9), a sliding groove is formed in the bottom supporting seat (9), a sliding panel (10) is arranged in the sliding groove in a sliding mode, the movable sliding rod (15) is fixed to the top of the sliding panel (10), the movable sliding plate (16) is fixed to the end portion of the movable sliding rod (15), the movable sliding plate (16) is connected with the bottom supporting seat (9) in a sliding mode, the buffer spring (17) is fixed to the top of the movable sliding plate (16), the other end of the buffer spring (17) is fixed to the top of the bottom supporting seat (9), the adjusting supporting plate (18) is fixed to the side edge of the sliding panel (10), the adjustable supporting plate is characterized in that a driving rack (13) is uniformly arranged on the adjusting supporting plate (18), a driving gear (14) is arranged inside the bottom supporting seat (9) through a power part, and the driving gear (14) is meshed with the driving rack (13) in a connecting mode.
5. The preparation device of the resistance-reducing probiotic fermented feed according to claim 1, characterized in that: inside structure of smashing includes the unloading control, smashes flabellum (28), drive pivot (29), smashes supporting seat (20) and two-way motor (21), jar body (8) bottom is provided with the unloading control, jar body (8) inside intermediate position is fixed with two-way motor (21) through horizontal support, just two-way motor (21) both sides rotate respectively and are provided with drive pivot (29) and unloading control, be fixed with on drive pivot (29) and smash supporting seat (20), smash and evenly be fixed with on supporting seat (20) and smash flabellum (28).
6. The preparation device of the resistance-reducing probiotic fermented feed according to claim 5, characterized in that: the blanking control part comprises a bottom blanking port (19), a rotating plate (25), a chassis (26), internal teeth (27), a bottom gear (22), a rotating chassis (23) and a connecting rod (24), a turntable (26) is fixed at the bottom of the tank body (8), the turntable (26) is provided with the bottom blanking port (19), the bottom of the tank body (8) is provided with a blanking port (12), the blanking port (12) is communicated with the bottom blanking port (19), two sides of the bidirectional motor (21) are respectively provided with a driving rotating shaft (29) and a bottom rotating shaft in a rotating mode, the bottom rotating shaft is arranged at the output end of the bidirectional motor (21) through an automatic clamping part, the end part of the bottom rotating shaft is fixed with the bottom gear (22), the chassis (26) is provided with the rotating plate (25) in a rotating mode, the rotating plate (25) is sector and is arranged on the rotating chassis (23) in a rotating mode through the connecting rod (24), the rotating chassis (23) is internally provided with internal teeth (27), and the internal teeth (27) are meshed with the bottom gear (22).
7. An anti-reducing probiotic fermented feed is characterized in that: the preparation is carried out by the preparation device of the resistance-reducing probiotic fermented feed as claimed in any one of claims 1-6, and the microbial agent prepared by microbial culture is mixed with the fermented feed and fermented;
the microbial culture preparation comprises the steps of respectively inoculating lactobacillus casei DF-3, lactobacillus delbrueckii JY-11 and enterococcus faecalis GY-9 to an MRS solid slant culture medium to be cultured to obtain a seed slant; respectively inoculating seed slopes of lactobacillus casei DF-3, lactobacillus delbrueckii JY-11 and enterococcus faecalis GY-9 in an MRS liquid culture medium to obtain respective liquid seed liquid; performing liquid expanded culture of strains, and respectively inoculating Lactobacillus casei DF-3, Lactobacillus delbrueckii JY-11 and enterococcus faecalis GY-9 into a 50L fermentation tank for culture; the volume ratio of the microbial agent for enlarged culture is 1: 1: 1, and mixing uniformly to obtain the novel resistance-reducing probiotic preparation.
8. A preparation method of an anti-reducing probiotic fermented feed is characterized by comprising the following steps: the preparation method is carried out by the preparation device of the resistance-reducing probiotic fermented feed as claimed in any one of claims 1 to 6, and comprises the following specific steps:
s1, preparation of a microbial preparation: 1. respectively inoculating lactobacillus casei DF-3, lactobacillus delbrueckii JY-11 and enterococcus faecalis GY-9 to an MRS solid slant culture medium to culture to obtain a seed slant; 2. respectively inoculating the seed slopes into an MRS liquid culture medium to obtain respective liquid seed solutions; 3. respectively inoculating the liquid seed solutions into 50L fermentation tanks for culture; 4. and (3) mixing the microbial agent subjected to amplification culture in a volume ratio of 1: 1: 1, uniformly mixing to obtain a novel antibiotic-reducing probiotic preparation;
S2, preparation of the probiotic fermented feed: putting alfalfa, corn straw and quinoa straw into a grinder according to a certain proportion, grinding to a length of 2.0-2.5mm, and then adding and mixing rapeseed meal according to a certain proportion to obtain a feed fine material;
s3, fermenting the fermented feed: adding a microbial preparation into the probiotic fermented feed according to 1-2% of the total weight of the probiotic fermented feed, adding water, adjusting the water content in the feed to 65-75%, placing the fermented material in a closed fermentation tank for fermentation for 30-40d, wherein the fermentation temperature is 40-45 ℃;
s4, granulating: putting the fermented feed in the step S3 into an evaporation tank for evaporation to ensure that the water content is lower than 30%, taking out the evaporated feed, putting the evaporated feed into an oven for drying to ensure that the water content is lower than 10%, and finally, transferring the evaporated feed into a granulator for granulation to prepare granulated feed.
9. The preparation method of the resistance-reducing probiotic fermented feed according to claim 8, wherein the slant culture medium culture conditions are as follows: culturing in a constant temperature incubator at 37 deg.C for 48-52 h; the culture conditions of the MRS liquid culture medium are as follows: culturing in a shaking incubator at 37 deg.C and 200r/min for 48-52 h; the culture conditions of the amplification culture are as follows: inoculating into 50L fermentation tank, wherein the culture medium is MRS liquid culture medium with an inoculum size of 10%, culturing at 37 deg.C and 200r/min for 48-52h with effective viable bacteria number of 5 × 10 8CFU/mL or above; the temperature of the evaporation zone in the evaporation tank is 55-65 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210246719.8A CN114747656A (en) | 2022-03-14 | 2022-03-14 | Resistance-reducing probiotic fermented feed, preparation method and preparation device thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210246719.8A CN114747656A (en) | 2022-03-14 | 2022-03-14 | Resistance-reducing probiotic fermented feed, preparation method and preparation device thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114747656A true CN114747656A (en) | 2022-07-15 |
Family
ID=82326377
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210246719.8A Pending CN114747656A (en) | 2022-03-14 | 2022-03-14 | Resistance-reducing probiotic fermented feed, preparation method and preparation device thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114747656A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115568604A (en) * | 2022-10-24 | 2023-01-06 | 遵义师范学院 | Preparation device of mulberry twig and mulberry leaf composite ration for artificial silkworm breeding |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012228218A (en) * | 2011-04-27 | 2012-11-22 | Osaka Prefecture | Fermented feed and method for producing the same |
| WO2014170233A2 (en) * | 2013-04-18 | 2014-10-23 | Chr. Hansen A/S | Method for improving milk quality in milk-producing animals |
| CN209135447U (en) * | 2018-03-28 | 2019-07-23 | 昆明饲宝饲料有限责任公司 | A kind of feed fermentation device |
| CN110959746A (en) * | 2019-12-27 | 2020-04-07 | 洛阳希望生物科技有限公司 | Probiotic fermented feed and preparation method thereof |
| CN111616388A (en) * | 2020-05-29 | 2020-09-04 | 山东省畜牧总站(山东省种畜禽质量测定站) | Device for preparing fermented feed for meat donkeys by using corn straws and vinasse and preparation method of device |
| CN212335167U (en) * | 2020-04-29 | 2021-01-12 | 赤峰四海同心生物科技有限公司 | Drying system of fermented feed |
| CN112970940A (en) * | 2021-04-20 | 2021-06-18 | 湖南普菲克生物科技有限公司 | Preparation process for producing antibacterial fermented feed by compounding multiple strains |
| CN113122456A (en) * | 2021-04-16 | 2021-07-16 | 湖南普菲克生物科技有限公司 | Preparation process of fermentation microbial inoculum for fermenting feed and auxiliary mixed fermentation tank thereof |
| CN215917655U (en) * | 2021-09-13 | 2022-03-01 | 青岛英伟生物技术有限公司 | A reducing mechanism for pig feed production |
-
2022
- 2022-03-14 CN CN202210246719.8A patent/CN114747656A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012228218A (en) * | 2011-04-27 | 2012-11-22 | Osaka Prefecture | Fermented feed and method for producing the same |
| WO2014170233A2 (en) * | 2013-04-18 | 2014-10-23 | Chr. Hansen A/S | Method for improving milk quality in milk-producing animals |
| CN209135447U (en) * | 2018-03-28 | 2019-07-23 | 昆明饲宝饲料有限责任公司 | A kind of feed fermentation device |
| CN110959746A (en) * | 2019-12-27 | 2020-04-07 | 洛阳希望生物科技有限公司 | Probiotic fermented feed and preparation method thereof |
| CN212335167U (en) * | 2020-04-29 | 2021-01-12 | 赤峰四海同心生物科技有限公司 | Drying system of fermented feed |
| CN111616388A (en) * | 2020-05-29 | 2020-09-04 | 山东省畜牧总站(山东省种畜禽质量测定站) | Device for preparing fermented feed for meat donkeys by using corn straws and vinasse and preparation method of device |
| CN113122456A (en) * | 2021-04-16 | 2021-07-16 | 湖南普菲克生物科技有限公司 | Preparation process of fermentation microbial inoculum for fermenting feed and auxiliary mixed fermentation tank thereof |
| CN112970940A (en) * | 2021-04-20 | 2021-06-18 | 湖南普菲克生物科技有限公司 | Preparation process for producing antibacterial fermented feed by compounding multiple strains |
| CN215917655U (en) * | 2021-09-13 | 2022-03-01 | 青岛英伟生物技术有限公司 | A reducing mechanism for pig feed production |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115568604A (en) * | 2022-10-24 | 2023-01-06 | 遵义师范学院 | Preparation device of mulberry twig and mulberry leaf composite ration for artificial silkworm breeding |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101139560B (en) | Process for producing feeding micro-ecological preparation by solid fermentation of brewery mash with various bacterium and fermentation culture medium thereof | |
| CN102093967B (en) | Mink source enterococcus faecium and application thereof | |
| CN103484288B (en) | Method for improving effect of medicinal liquor and probiotic composition | |
| CN101461453B (en) | Composite microbial feed additive for beasts and birds | |
| CN102559545B (en) | Composite micro-ecological preparation for aquatic product and premix thereof | |
| CN106858066B (en) | Additive for synergistically promoting proliferation and colonization of intestinal probiotics and application method thereof | |
| CN108949513A (en) | A kind of microbial ferment device | |
| CN101603016A (en) | Live lactobacillus composite ecological microbial agent | |
| CN106635886A (en) | Method for reducing egg cholesterol by utilizing lactobacillus paracasei of bile salt producing hydrolase | |
| CN107043722A (en) | One plant of pig source lactobacillus reuteri and its solid-state probiotics of preparation | |
| CN110317753A (en) | A kind of bacillus megaterium microbial inoculum, liquid fermentation process and its application | |
| CN103283948A (en) | Bifidobacterium bifidum-oriented microecological preparation | |
| CN101138392B (en) | Micro-zoology preparations for feeding | |
| Lustrato et al. | Cheese whey recycling in traditional dairy food chain: effects of vinegar from whey in dairy cow nutrition | |
| CN101857469A (en) | Preparation process for golf course lawn slow-release bio-organic fertilizer produced by three continuous fermentations | |
| KR20200087388A (en) | Animal feed additive using agricultural wastes and process for preparing the same | |
| CN114747656A (en) | Resistance-reducing probiotic fermented feed, preparation method and preparation device thereof | |
| CN102406068A (en) | Method for preparing microbial fermented feed capable of keeping intestinal health of pigs | |
| CN103283974A (en) | A method for producing a probiotic agent of live Lactobacillus bulgaricus | |
| CN101225364A (en) | Discovery of forage grass attached lactobacillus plantarum and enterococcus mundtii and use thereof | |
| CN104830711A (en) | Solid fermentation process of bacillus amyloliquefaciens feed additive | |
| CN117016691A (en) | Probiotic feed additive for improving pork quality | |
| CN109938185A (en) | Ammonia-nitrogen content and there is the feed addictive for promoting digestive function in a kind of reduction pig manure | |
| CN1579232A (en) | Multi-health-bateria compound nutrient freezed powder capsule and its making method | |
| CN107198038B (en) | Chlorella powder and spirulina powder composition and feed prepared from composition and used for breeding healthy pigs |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |