CN114747656A - Antibiotic-reduced probiotic fermented feed, preparation method and preparation device thereof - Google Patents

Abstract

The invention discloses an anti-resistance-reduced probiotic fermented feed, which relates to the technical field of feed processing. There is a fermented bacteria liquid drain pipe, the fermentation bacteria liquid drain pipe is connected to one side of the top of the feed fermentation tank, a flow meter is provided on the fermentation bacteria liquid drain pipe, and the discharge pipe of the feed fermentation tank is connected to To the raw material liquid inlet of the evaporation tank, the material pulverizer and the mixer are both arranged beside the feed fermentation tank. Using the feed of the present invention can improve the contents of serum albumin, globulin and total protein in the blood of the cubs, and the contents of alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase in the serum are lower than those of the cubs fed with the feed purchased in the market. Feed is beneficial to the various metabolic and physiological activities in the pups.

Description

Antibiotic-reduced probiotic fermented feed, preparation method and preparation device thereof

technical field

The invention relates to the technical field of feed processing, in particular to an anti-resistance-reduced probiotic fermented feed, a preparation method and a preparation device thereof.

Background technique

The immune system of young animals is weak. In farms that raise a large number of animals, animals start to feed them with feed when they are a few months old. The cubs have weak ability to digest the feed, and when they cannot digest or digest slowly, it will cause the cubs to absorb and slow metabolism, affecting development. In China, pig and sheep pups need to be treated with feed before they can be eaten. To make the feed easy to digest and absorb, it must also be able to supplement enough nutrients, especially in the process of milk feeding and feed feeding, the feed should be the same as breast milk. It is as easy to absorb, and it cannot make the cubs develop greater resistance. Therefore, resistance-reduced diets are very important for the feeding of pups.

SUMMARY OF THE INVENTION

The present invention aims to solve one of the technical problems in the related art at least to a certain extent. Therefore, an object of the present invention is to provide an anti-resistance-reduced probiotic fermented feed, a preparation method and a preparation device thereof, so as to solve the problems existing in the background technology.

A preparation device for reducing resistance type probiotic fermented feed is characterized in that: comprising:

The strain fermenter is used to ferment strains, and also includes:

Feed fermenter for fermenting feed, also including:

Material pulverizer for pulverizing feed raw materials; also includes:

Mixer for mixing feed and cultures; also includes:

Evaporation tank, used to provide high temperature conditions for the fermentation process of feed; also includes:

an oven for drying the resulting feed;

The bottom of the strain fermentation tank is provided with a fermented bacteria liquid drain pipe, the fermentation bacteria liquid drain pipe is connected to one side of the top of the feed fermentation tank, a flow meter is arranged on the fermentation bacteria liquid drain pipe, and the feed The discharge pipe of the fermentation tank is connected to the inlet of the raw material liquid of the evaporation tank, and the material pulverizer and the mixer are both arranged beside the feed fermentation tank.

In some embodiments of the present invention, the feed fermentation tank includes a tank body, a base, a support adjustment structure, a feeding port, a top inlet port and an internal crushing structure, and the base is provided with a tank body through the support adjustment structure, The support adjustment structure is used to control the unloading, the top of the tank body is provided with a top inlet port, the bottom of the tank body is provided with a discharge port, and the interior of the tank body is provided with an internal crushing structure for The feed material is further crushed.

In some embodiments of the present invention, the support adjustment structure includes a sliding panel, a bottom support base and a power component, the tank body is fixed on the bottom support base, and a sliding groove is opened on the bottom support base, so A sliding panel is slidably arranged inside the sliding slot, and a power component is arranged on the side of the sliding panel.

In some embodiments of the present invention, the power component includes a driving rack, a driving gear, a moving support rod, a moving sliding plate, a buffer spring and an adjusting support plate, the tank body is fixed on a bottom support seat, and the bottom A sliding slot is provided on the support base, a sliding panel is slidably arranged inside the sliding slot, a movable sliding rod is fixed on the top of the sliding panel, a movable sliding plate is fixed at the end of the movable sliding rod, and the movable sliding plate and the The bottom support base is slidably connected, a buffer spring is fixed on the top of the moving sliding plate, the other end of the buffer spring is fixed on the top of the bottom support base, an adjustment support plate is fixed on the side of the sliding panel, and the adjustment support A driving rack is evenly arranged on the plate, a driving gear is arranged inside the bottom support seat through a power element, and the driving gear and the driving rack are meshed and connected.

In some embodiments of the present invention, the internal crushing structure includes a blanking control member, a crushing fan blade, a driving shaft, a crushing support seat and a bidirectional motor, and the bottom of the tank is provided with a blanking control member, so A two-way motor is fixed in the middle position of the tank body through a transverse support, and a driving shaft and a blanking control member are respectively rotated on both sides of the two-way motor. A breaking support seat is fixed on the driving shaft, and the breaking support Shattered fan blades are evenly fixed on the seat.

In some embodiments of the present invention, the feeding control member includes a bottom feeding port, a rotating plate, a chassis, internal teeth, a bottom gear, a rotating chassis and a connecting rod, and a turntable is fixed at the bottom of the tank body, and the turntable is A bottom discharge port is opened on the top, a discharge port is opened at the bottom of the tank body, and the discharge port and the bottom discharge port are connected. The two sides of the two-way motor are respectively provided with a driving shaft and a bottom shaft. The bottom rotating shaft is set at the output end of the two-way motor through an automatic clip, the bottom gear is fixed at the end of the bottom rotating shaft, and a rotating plate is rotated on the chassis. On the chassis, internal teeth are arranged inside the rotating chassis, and the internal teeth are meshed and connected with the bottom gear.

An antibiotic-reduced probiotic fermented feed is obtained by mixing and fermenting a microbial inoculum prepared by microbial culture and a fermented feed;

The microorganism culture preparation adopts Lactobacillus casei DF-3, Lactobacillus delbrueckii JY-11 and Enterococcus faecalis GY-9 to inoculate the MRS solid slant medium to cultivate seed slants respectively; The seed slants of Lactobacillus JY-11 and Enterococcus faecalis GY-9 were respectively inoculated in MRS liquid medium to obtain their respective liquid seed liquids; 11 and Enterococcus faecalis GY-9 were respectively inoculated in a 50L fermenter for culture; the expanded cultured microbial inoculants were mixed uniformly at a volume ratio of 1:1:1 to obtain a new type of anti-resistance-reduced probiotic preparation.

A preparation method of anti-resistance type probiotic fermented feed, the specific steps are as follows:

S1, the preparation of microbial preparations: 1. Inoculate Lactobacillus casei DF-3, Lactobacillus delbrueckii JY-11 and Enterococcus faecalis GY-9 on MRS solid slant medium and cultivate seed slants respectively; 2. Separate the seed slants Inoculate in MRS liquid medium to obtain respective liquid seed liquids; 3. Inoculate the liquid seed liquids in 50L fermenters respectively for cultivation; 4. Mix the expanded microbial inoculum by volume ratio 1:1:1, A novel anti-resistance-reducing probiotic preparation is obtained;

S2. Preparation of probiotic fermented feed: put alfalfa grass, corn stalk and quinoa stalk together in a pulverizer in proportion, pulverize to a length of 2.0-2.5mm, and then add and mix rapeseed meal in proportion to obtain a feed refined material ;

S3. Fermentation of fermented feed: add the microbial preparation to the probiotic fermented feed according to 1%-2% of the total weight of the probiotic fermented feed, add water, adjust the moisture content in the feed to 65-75%, and place the fermented material in a closed fermentation tank Fermentation 30-40d, fermentation temperature 40-45℃;

S4, granulation: put the fermented feed in step S3 into an evaporation tank for evaporation, so that its moisture content is lower than 30%, take out the evaporated feed and put it into an oven for drying, so that its moisture content is lower than 10%, Finally, it is moved into a granulator for granulation to make pellet feed.

In other embodiments of the present invention, the culture conditions of the slant medium are: culturing in a constant temperature incubator at 37°C for 48-52 hours; the culture conditions of the MRS liquid medium are: the temperature is 37°C, and the rotational speed is Culture in a 200r/min shaking incubator for 48-52h; the culture conditions for the expanded culture are: inoculated in a 50L fermenter, the medium is MRS liquid medium, the inoculum volume is 10%, the temperature is 37°C, and the rotational speed is After culturing at 200r/min for 48-52h, the number of effective viable bacteria is above 5×10 ⁸ CFU/mL; the temperature of the evaporation zone in the evaporation tank is 55-65°C.

In the present invention, the use of the feed of the present invention can increase the contents of serum albumin, globulin and total protein in the blood of the cubs, and the contents of alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase in the serum are lower than those of the cubs fed with the market purchased feed , the feed of the present invention is beneficial to the progress of various metabolism and physiological activities in the cubs; the application can adjust the height of the tank by using the support adjustment structure, so as to avoid waste due to splashing during feeding , and the bottom collection box can be easily pulled out; this application adopts the corresponding smashing to combine with the blanking control component, so that the action of the bidirectional motor can be used to further smash during the blanking process, so as to be able to rotate In the process of cutting, and the material can be fully broken under the action of gravity, if the breaking effect is not good, the automatic clamping part can also be used to control the chassis not to rotate, so as to block the bottom discharge opening.

Description of drawings

The accompanying drawings are used to provide a further understanding of the present invention, and constitute a part of the specification, and are used to explain the present invention together with the embodiments of the present invention, and do not constitute a limitation to the present invention. In the attached image:

FIG. 1 is a schematic diagram of the structure layer of a device for preparing an anti-resistance type probiotic fermented feed proposed by the present invention.

FIG. 2 is an experimental comparison diagram of the preparation of an anti-resistance-reduced probiotic fermented feed proposed by the present invention.

FIG. 3 is a schematic diagram of a material fermentation tank of a device for preparing an anti-resistance type probiotic fermented feed proposed by the present invention.

Fig. 4 is a schematic diagram of the support and adjustment structure of a preparation device for reducing resistance type probiotic fermented feed proposed by the present invention.

Fig. 5 is a schematic diagram of a feeding port at the bottom of a device for preparing an anti-resistance-reduced probiotic fermented feed proposed by the present invention.

FIG. 6 is a schematic diagram of the internal crushing of an anti-resistance type probiotic fermented feed preparation device proposed by the present invention.

In the figure: 1. Bacteria fermentation tank; 2. Flow meter; 3. Feed fermentation tank; 4. Evaporation tank; 5. Material pulverizer; 6. Mixer; 7. Top inlet; 8. Tank body; 9 , bottom support seat; 10, sliding panel; 11, base; 12, feeding opening; 13, driving rack; 14, driving gear; 15, moving support rod; 16, moving sliding plate; 17, buffer spring; 18, Adjustment support plate; 19. Bottom feeding port; 20. Breaking support seat; 21. Two-way motor; 22-bottom gear; 23. Rotating chassis; 24. Connecting rod; 25. Rotating plate; 26. Chassis; 27. Internal Teeth; 28, break the fan blade; 29, break the shaft.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, but not all of the embodiments.

Example 1: See Figures 1-6

Preparation of an anti-resistance-reducing probiotic preparation

(1) Preparation of microbial slant seeds: Lactobacillus casei DF-3, Lactobacillus delbrueckii JY-11 and Enterococcus faecalis GY-9 were inoculated into MRS solid slant medium, and after culturing at 37°C for 48-52 hours, the Seed bevel.

The MRS solid slant culture medium is: casein peptone 10g, beef extract 10g, yeast powder 5g, glucose 5g, sodium acetate 5g, diammonium citrate 2g, Tween 801g, dipotassium hydrogen phosphate 2g, magnesium sulfate heptahydrate 0.2g, 0.05 g of manganese sulfate, 20 g of calcium carbonate, 20 g of agar, 1000 mL of distilled water, pH 6.8. Autoclave at 121°C for 20min.

(2) Cultivation of microbial liquid seeds: Lactobacillus casei DF-3, Lactobacillus delbrueckii JY-11 and Enterococcus faecalis GY-9 were respectively inoculated in MRS liquid medium at a temperature of 37°C and a rotation speed of 200r/min After shaking culture for 48-52h, it is liquid seed.

(3) Bacterial liquid expansion culture: Lactobacillus casei DF-3, Lactobacillus delbrueckii JY-11 and Enterococcus faecalis GY-9 were inoculated in a 50L fermenter respectively, the medium was MRS liquid medium, and the inoculation amount was 10 %, incubate for 48-52 hours at a temperature of 37°C and a rotation speed of 200 r/min, and stop the fermentation when the number of effective viable bacteria in the above-mentioned three kinds of lactic acid bacteria is above 5×10 ⁸ CFU/mL.

(4) Preparation of the microbial preparation: the microbial inoculants respectively expanded and cultured in step (3) are mixed uniformly in a volume ratio of 1:1:1, to obtain a new type of anti-resistance-reduced probiotic preparation.

Embodiment 2: Please refer to Figures 1-6

Preparation of an anti-resistance-reduced probiotic fermented feed

The raw materials of the fermented feed are: alfalfa grass 40-50%, fresh corn straw 20-35%, quinoa straw 20-35%, rapeseed meal 5-10%.

(1) Put the alfalfa grass into the pulverizer and pulverize; the corn stalk and the quinoa straw are put into the pulverizer together, and pulverized to a length of about 2.0-2.5mm; the rapeseed meal is added to the pulverized material by weight, and put into the mixer After internal mixing, feed refined material is obtained;

(2) adding the composite microbial preparation (anti-resistance-reducing probiotic preparation) prepared in Example 1 in a proportion of 1% of the total weight of the refined material and mixing it, adding water to adjust the water content in the feed to be 65-75%;

(3) placing the fermented material obtained in step (2) in an airtight environment (feed fermenter) for 30-40 d fermentation to obtain a new type of anti-resistance-reduced probiotic fermented feed.

Test 1

Twenty-four lambs with similar body weight and good health status of about 3 months were selected for the experiment and randomly divided into 3 groups with 8 lambs in each group, namely experimental group 1, experimental group 2 and control group. The lambs in each group were kept under the same conditions, and were fed with ewes and routinely lactated. During supplementary feeding, lambs and ewes were separated, and each was fed supplementary feed. Test group 1 was supplemented with anti-resistance-reduced probiotic fermented feed, and test group 2 was supplemented with commercial feed (Closan, Jianming (China) Technology Co., Ltd.), The control group was not fed. Lambs use supplementary feeding stalls, and only the lambs are allowed to come and go freely. During the pre-trial period, the lambs are subjected to health check, deworming, epidemic prevention treatment, numbering, fixed sheep house, and then transferred to the positive trial period after adapting to the environment. The pre-trial period is 7 days and the trial period is 28 days. Feeding at 06:00 and 18:00 every day, ad libitum access to food and water ad libitum. Experiments Lamb body weights were measured on days 0, 7, 14 and 28 of the experiment.

Table 1. Effects of probiotic supplementation on growth performance of lambs

Test group 1 Test group 2 control group Lamb body weight/Kg at the beginning of the experiment 4.22±0.43 4.81±0.32 4.57±0.48 Body weight of lambs at the end of the test/Kg 16.97±2.57 17.04±2.48 16.33±2.65 Test weight gain/Kg 12.75±1.34 12.23±2.04 11.76±2.13 Average daily gain/Kg 0.46±0.03 0.44±0.05 0.42±0.03 Material to weight ratio 0.29 0.37 0.43

Test results: From Table 1, it can be seen that the addition of probiotics helps to improve the growth performance of lambs. The weight gain, average daily gain and feed-to-weight ratio of the anti-resistance-reduced probiotic fermented feed of the present invention are higher than those of the commercial group and the control group. .

Test Example 2

The management of lamb feeding is the same as above. At the end of the experiment, 10 mL of jugular venous blood of lambs in each group was collected, and the serum was separated after standing, and stored at -20°C to determine the relevant blood biochemical indicators.

Table 2. Effects of probiotic supplementation on blood biochemical indexes of lambs

Test group 1 Test group 2 control group Total protein g/L 46.69±2.13 43.76±2.26 43.18±2.47 Albumin g/L 33.48±2.42 32.28±2.44 30.19±2.39 globulin g/L 13.48±1.85 13.04±1.48 11.33±1.75 Alanine aminotransferase U/L 8.47±3.48 8.62±3.35 9.84±2.68 Aspartate aminotransferase U/L 75.39±11.46 80.45±10.84 83.93±11.54 Alkaline Phosphatase U/L 514.48±85.38 507.33±95.38 615.37±98.67 Total cholesterol mmol/L 2.32±0.47 2.11±0.31 2.08±0.43 Urea nitrogen mmol/L 7.03±1.38 7.68±2.04 7.48±2.42 Glucose mmol/L 5.33±1.31 4.95±1.48 5.03±1.04

The results showed that the serum albumin, globulin and total protein contents of lambs in the probiotics group were higher than those of the control group; the serum alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase contents of the lambs in the probiotics group were lower than those of the control group; the probiotics group The total cholesterol content is higher than that of the control group; wherein the anti-resistance-reduced probiotic fermented feed of the present invention is generally better than the commercial control. The lamb serum urea nitrogen content was the lowest in test group 1; the serum glucose content was the highest in test group 1, indicating that the anti-resistance-reduced probiotic fermented feed of the present invention is beneficial to various metabolism and physiological activities in lambs.

Test Example 3

The management of lamb feeding is the same as above. At the end of the experiment, fresh fecal samples of lambs in each group were collected and stored at -80°C. The changes in fecal microbial flora were determined by high-throughput sequencing.

The results showed that: compared with the control group, the changes of the fecal microflora of the lambs in the probiotic group were mainly increased Bifidobacterium, the number of Lactobacillus increased, and the number of Escherichia coli decreased; , the number of Bifidobacterium and Lactobacillus microorganisms increased significantly, indicating that the fermented feed of the present invention is beneficial to the increase of the number of beneficial microorganisms of lactic acid bacteria in the intestinal tract of lambs, and the decrease of the number of pathogenic microorganisms such as Escherichia coli.

For the above-mentioned feed preparation device, please refer to FIG. 1, the present application includes a strain fermentation tank 1, a feed fermentation tank 3, a material pulverizer 5, a mixer 6, an evaporation tank 4 and an oven, wherein the strain fermentation tank 1 is a In order to ferment the bacterial species required by the feed, a fermentation bacterial liquid drain is arranged on the bottom of the bacterial species fermentation tank 1, and the fermented bacterial liquid drain is connected to one side of the top of the feed fermentation tank 3, so that fermentation can be obtained. The bacterial liquid reaches the inside of the feed fermentation tank 3 through the fermented bacterial liquid discharge pipe, wherein a flowmeter 2 is provided on the fermentation bacterial liquid discharge pipe, wherein the flowmeter 2 is to detect the flow of the bacterial liquid entering, in the feed fermentation tank 3. The discharge pipe is connected to the inlet of the raw material liquid of the evaporation tank 4, and the material pulverizer 5 and the mixer 6 are both arranged beside the feed fermentation tank 3, and are used to crush the raw material obtained by the material pulverizer 5 and fill it into the feed. Inside the fermenter 3.

As a further embodiment of the present application, please refer to FIG. 1 and FIG. 3, wherein the feed fermenter 3 includes a tank body 8, a base 11, a support adjustment structure, a feeding port 12, a top inlet port 7 and an internal crushing structure. The base 11 is provided with a tank body 8 through a support adjustment structure, and the longitudinal height of the tank body 8 can be adjusted by using the support adjustment structure, and the top of the tank body 8 is provided with a top inlet port 7, and the top inlet port 7 is used to enter the required raw materials, and A feeding port 12 is arranged at the bottom of the tank body 8, and the finished product is discharged by using the feeding port 12. An internal crushing structure is arranged inside the tank body 8, and the raw materials are crushed by the internal crushing structure. In this embodiment, the raw materials are firstly entered from the top inlet 7, and the raw materials are broken inside the tank body 8 through the internal crushing structure. After the crushing, the vertical height of the tank body 8 is adjusted by using the support adjustment structure. Unloading can be facilitated, and by using the longitudinally moving tank 8 , the collecting tank for loading the finished feed can be removed from the unloading port 12 .

As a further embodiment of the present application, please refer to FIG. 1 , FIG. 3 and FIG. 4 , wherein in order to realize the adjustment of the overall height of the tank body 8 , the support adjustment structure includes a sliding panel 10 , a bottom support seat 9 , a driving rack 13 , The driving gear 14, the moving support rod 15, the moving sliding plate 16, the buffer spring 17 and the adjusting support plate 18, firstly fix the tank body 8 on the bottom support seat 9, in order to realize the longitudinal height adjustment of the tank body 8, so the bottom support The seat 9 is provided with a sliding groove, and a sliding panel 10 is slidably arranged inside the sliding groove. The length of the sliding panel 10 and the bottom support seat 9 is used to control the height of the tank 8. The top of the sliding panel 10 is fixed with a Move the sliding rod 15, the end of the moving sliding rod 15 is fixed with a moving sliding plate 16 and the moving sliding plate 16 is slidably connected with the bottom support base 9, that is, when the moving sliding plate 16 moves longitudinally, the top of the moving sliding plate 16 is used to fix a buffer The spring 17 and the other end of the buffer spring 17 are fixed on the top of the bottom support seat 9 to buffer the position of the sliding panel 10. When the power element fails, the buffer tank 8 and the sliding panel can be operated under the action of the buffer spring 17. 10. An adjustment support plate 18 is fixed on the side, and a drive rack 13 is evenly arranged on the adjustment support plate 18. A drive gear 14 is provided inside the bottom support base 9 through a power member, and the drive gear 14 and the drive rack 13 are meshed and connected, that is, drive The gear 14 is rotated under the action of the power element, so that the positions of the driving gear 14 and the driving rack 13 are moved relatively, so that the longitudinal height of the bottom support seat 9 can be adjusted;

In order to control the crushing of the raw materials and control the cutting of the crushed raw materials, in this embodiment, referring to FIG. 1 , FIG. 3 , FIG. 5 and FIG. 6 , the internal crushing structure includes a bottom discharging port 19 , rotating plate 25, chassis 26, internal teeth 27, crushing fan blades 28, driving shaft 29, crushing support seat 20, bidirectional motor 21, bottom gear 22, rotating chassis 23 and connecting rod 24, that is, first in the tank 8 A turntable 26 is fixed at the bottom, and a bottom discharge port 19 is opened on the turntable 26 to realize unloading. The bottom of the tank body 8 is provided with a discharge port 12, and the discharge port 12 and the bottom discharge port 19 are connected. When When there is no cover on the bottom feeding port 19, the feeding can be carried out. The inner middle position of the tank body 8 is fixed with a two-way motor 21 through a horizontal bracket, and the two sides of the two-way motor 21 are respectively rotated and provided with a driving shaft 29 and a bottom shaft. , the bottom rotating shaft is set at the output end of the two-way motor 21 through the automatic clamping part, that is, whether the two-way motor 21 drives the bottom rotating shaft to rotate by using the automatic clamping part, wherein the driving rotating shaft 29 is evenly provided with a crushing fan blade 28, and the use of crushing The fan blade 28 smashes the raw materials, wherein the bottom gear 22 is fixed at the end of the bottom rotating shaft, and a rotating plate 25 is arranged on the chassis 26 to rotate. Internal teeth 27 are provided inside, and the internal teeth 27 are engaged with the bottom gear 22, that is, when the bottom gear 22 rotates, the internal teeth 27 can be driven to rotate, thereby driving the rotating plate 25 to rotate, so that the rotating plate 25 can be rotated. The bottom feeding port 19 is separated from the bottom feeding port 19, so as to facilitate feeding, and the bottom feeding port 19 can be placed on the rotating chassis 23 by using the rotating chassis 23 and the rotating plate 25, so as to facilitate feeding. In order to further facilitate feeding , a bucket-shaped shield can be provided on the chassis 26 , and the output end of the bucket-shaped shield is arranged on the bottom discharge port 19 .

The working raw materials of the device: the strain fermentation tank 1 is a device for fermenting the strains required by the feed, and a fermentation bacteria liquid drain pipe is arranged on the bottom of the strain fermentation tank 1, and the fermentation bacteria liquid drain pipe is connected to the One side of the top of the feed fermentation tank 3, so that the bacterial liquid obtained by fermentation reaches the inside of the feed fermentation tank 3 through the fermented bacterial liquid discharge pipe. Connected to the raw material liquid inlet of the evaporation tank 4, the material pulverizer 5 and the mixer 6 are both arranged beside the feed fermentation tank 3, and are used to crush the raw materials obtained by the material pulverizer 5 and fill it into the feed fermentation tank 3. Inside, when the raw material is broken, the raw material enters from the top inlet 7, and the raw material is broken inside the tank body 8 through the internal breaking structure. After the breaking, the vertical height of the tank body 8 is realized by using the support adjustment structure. To adjust, lift the tank body 8, place the collection tank loaded with the finished feed at the bottom of the tank body 8, and then lower the collection tank by using the longitudinal height to make the collection tank close to the tank body 8 to avoid splashing. When the collection tank is full, the tank body 8 is lifted to facilitate pulling out the collection tank. Meanwhile, in the present application, the feed fermentation tank 3 is integrated with the crushing function of the material crusher 5, which can save space.

The above description is only a preferred embodiment of the present invention, but the protection scope of the present invention is not limited to this. The equivalent replacement or change of the inventive concept thereof shall be included within the protection scope of the present invention.

Claims (9)

1. a preparation device of anti-resistance type probiotic fermented feed, is characterized in that: comprising: A strain fermentation tank (1), used for fermenting strains, further comprising: The feed fermenter (3) is used for fermenting the feed, further comprising: A material pulverizer (5), which is used for pulverizing feed raw materials; it also includes: A mixer (6) for mixing feed and bacteria; also includes: The evaporation tank (4) is used to provide high temperature conditions for the fermentation process of the feed; it also includes: an oven for drying the resulting feed; The bottom of the bacterial strain fermentation tank (1) is provided with a fermentation bacteria liquid drain pipe, the fermentation bacteria liquid drain pipe is connected to one side of the top of the feed fermentation tank (3), and the fermentation bacteria liquid drain pipe is provided with a The flow meter (2), the discharge pipe of the feed fermentation tank (3) is connected to the raw material liquid inlet of the evaporation tank (4), and the material pulverizer (5) and the mixer (6) are both arranged in Next to the feed fermentation tank (3). 2. The device for preparing an anti-resistance-reduced probiotic fermented feed according to claim 1, wherein the feed fermentation tank (3) comprises a tank body (8), a base (11), a support adjustment structure, a lower A material port (12), a top inlet port (7) and an internal crushing structure, the base (11) is provided with a tank body (8) through a support adjustment structure, and the support adjustment structure is used to control the unloading, The top of the tank body (8) is provided with a top inlet port (7), the bottom of the tank body (8) is provided with a discharge port (12), and the tank body (8) is provided with an internal crushing structure, which is used for For further crushing of feed raw materials. 3 . The device for preparing an anti-resistance-reduced probiotic fermented feed according to claim 1 , wherein the support and adjustment structure comprises a sliding panel ( 10 ), a bottom support seat ( 9 ) and a power component, 3 . The tank body (8) is fixed on the bottom support seat (9), the bottom support seat (9) is provided with a sliding groove, and a sliding panel (10) is slidably arranged inside the sliding groove, and the sliding panel (10) ) is provided with a power component on the side. 4. The device for preparing an anti-resistance-reduced probiotic fermented feed according to claim 3, wherein the power component comprises a driving rack (13), a driving gear (14), a moving support rod (15), Move the sliding plate (16), the buffer spring (17) and the adjusting support plate (18), the tank body (8) is fixed on the bottom support seat (9), and the bottom support seat (9) is provided with a sliding groove A sliding panel (10) is slidably arranged inside the sliding slot, a movable sliding rod (15) is fixed on the top of the sliding panel (10), and a movable sliding plate (16) is fixed at the end of the movable sliding rod (15) And the moving sliding plate (16) is slidably connected to the bottom support seat (9), a buffer spring (17) is fixed on the top of the moving sliding plate (16), and the other end of the buffer spring (17) is fixed on the On the top of the bottom support seat (9), an adjustment support plate (18) is fixed on the side of the sliding panel (10), and a drive rack (13) is evenly arranged on the adjustment support plate (18). A drive gear (14) is provided inside the support base (9) through a power element, and the drive gear (14) is meshed with the drive rack (13). 5. The device for preparing an anti-resistance-reduced probiotic fermented feed according to claim 1, wherein the internal crushing structure comprises a blanking control member, a crushing fan blade (28), and a drive shaft (29) , break the support seat (20) and the bidirectional motor (21), the bottom of the tank body (8) is provided with a blanking control member, and the inner middle position of the tank body (8) is fixed with a bidirectional motor (21) through a transverse bracket , and the two sides of the two-way motor (21) are respectively provided with a driving shaft (29) and a blanking control member, and a crushing support seat (20) is fixed on the driving rotary shaft (29), and the crushing support base is The crushing fan blades (28) are evenly fixed on the (20). 6 . The device for preparing anti-resistance-reduced probiotic fermented feed according to claim 5 , wherein the feeding control member comprises a bottom feeding port ( 19 ), a rotating plate ( 25 ), and a chassis ( 26 ). 7 . , internal teeth (27), bottom gear (22), rotating chassis (23) and connecting rod (24), a turntable (26) is fixed on the bottom of the tank (8), and a bottom is opened on the turntable (26). A feeding port (19), a feeding port (12) is opened at the bottom of the tank body (8), and the feeding port (12) is communicated with the feeding port (19) at the bottom, and the bidirectional motor (21) A drive shaft (29) and a bottom shaft are respectively rotatably provided on both sides, and the bottom shaft is arranged at the output end of the bidirectional motor (21) through an automatic clamping piece, and a bottom gear (22) is fixed at the end of the bottom shaft, A rotating plate (25) is rotatably arranged on the chassis (26), the rotating plate (25) is rotatably arranged on the rotating chassis (23) in a fan shape through a connecting rod (24), and the rotating chassis (23) is internally arranged There are internal teeth (27), and the internal teeth (27) are engaged with the bottom gear (22). 7. An anti-resistance-reduced probiotic fermented feed, characterized in that: it is prepared by a device for preparing an anti-resistance-reduced probiotic fermented feed according to any one of claims 1-6, and the microbial inoculum prepared by microbial culture and The fermented feed is mixed and fermented; The microorganism culture preparation adopts Lactobacillus casei DF-3, Lactobacillus delbrueckii JY-11 and Enterococcus faecalis GY-9 to inoculate the MRS solid slant medium to cultivate seed slants respectively; The seed slants of Lactobacillus JY-11 and Enterococcus faecalis GY-9 were respectively inoculated in MRS liquid medium to obtain their respective liquid seed liquids; 11 and Enterococcus faecalis GY-9 were respectively inoculated in a 50L fermenter for culture; the expanded cultured microbial inoculants were mixed uniformly at a volume ratio of 1:1:1 to obtain a new type of anti-resistance-reduced probiotic preparation. 8. A method for preparing an anti-resistance-reduced probiotic fermented feed, characterized in that: the preparation is carried out by any one of the preparation devices of any one of claims 1-6, and the specific steps are as follows: S1, the preparation of microbial preparations: 1. Inoculate Lactobacillus casei DF-3, Lactobacillus delbrueckii JY-11 and Enterococcus faecalis GY-9 on MRS solid slant medium and cultivate seed slants respectively; 2. Separate the seed slants Inoculate in MRS liquid medium to obtain respective liquid seed liquids; 3. Inoculate the liquid seed liquids in 50L fermenters respectively for cultivation; 4. Mix the expanded microbial inoculum by volume ratio 1:1:1, A novel anti-resistance-reducing probiotic preparation is obtained; S2. Preparation of probiotic fermented feed: put alfalfa grass, corn stalk and quinoa stalk together in a pulverizer in proportion, pulverize to a length of 2.0-2.5mm, and then add and mix rapeseed meal in proportion to obtain a feed refined material ; S3. Fermentation of fermented feed: add the microbial preparation to the probiotic fermented feed according to 1%-2% of the total weight of the probiotic fermented feed, add water, adjust the moisture content in the feed to 65-75%, and place the fermented material in a closed fermentation tank Fermentation 30-40d, fermentation temperature 40-45℃; S4, granulation: put the fermented feed in step S3 into an evaporation tank for evaporation, so that its moisture content is lower than 30%, take out the evaporated feed and put it into an oven for drying, so that its moisture content is lower than 10%, Finally, it is moved into a granulator for granulation to make pellet feed. 9. The method for preparing anti-resistance-reduced probiotic fermented feed according to claim 8, wherein the culture conditions of the slant medium are: culture in a 37°C constant temperature incubator for 48-52 hours; the MRS liquid culture The culture conditions of the base are as follows: the temperature is 37°C, and the rotation speed is 200r/min in a shaking incubator for 48-52h; the culture conditions for the expanded culture are: inoculation in a 50L fermenter, the medium is MRS liquid medium, The inoculum amount is 10%, the temperature is 37°C, and the rotation speed is 200r/min for 48-52h, and the number of effective viable bacteria is above 5×10 ⁸ CFU/mL; the temperature of the evaporation zone in the evaporation tank is 55-65°C .

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