CN114747478B

CN114747478B - Composite breeding method for pasture

Info

Publication number: CN114747478B
Application number: CN202110053516.2A
Authority: CN
Inventors: 冯永德; 冯超; 张云丹
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-01-15
Filing date: 2021-01-15
Publication date: 2023-10-13
Anticipated expiration: 2041-01-15
Also published as: CN114747478A

Abstract

The application discloses a composite breeding method of pasture, which solves the technical problems that the pasture fed by the existing livestock is insufficient in nutritive value and insufficient in yield, and cannot meet the requirements of high-quality, high-safety and rich-nutrition pasture products. The application comprises the following steps: breeding female parent of new pasture variety; male parent of new forage grass variety, and new forage grass variety. The forage grass bred by the application has the advantages of high yield, rich nutrition, capability of replacing grain with grass, capability of replacing material with grass, and the like.

Description

Composite breeding method for pasture

Technical Field

The application relates to the technical field of breeding, in particular to a composite breeding method of pasture.

Background

At present, in order to promote growth and obtain a certain amount of meat, an additive feed feeding mode is generally adopted, so that on the one hand, malodorous excrement is generated to cause environmental protection, the meat quality of livestock is poor, and in addition, the feeding cost is increased. If the forage grass is completely adopted for feeding, insufficient nutrition can occur, the growth is slow, and the market supply of the forage grass is seriously insufficient.

For this reason, it is necessary to cultivate pastures with higher nutritional value and higher yield to meet the current requirements of high quality, high safety and nutrient rich livestock products.

Disclosure of Invention

The application aims to solve the technical problems that: the existing pasture raised by livestock is insufficient in nutritive value and insufficient in yield, and cannot meet the requirements of high-quality, high-safety and nutrient-rich pasture products.

The application can be realized by the following technical scheme:

a composite breeding method of pasture, comprising the following steps:

step 1: male parent for breeding new variety of pasture

Performing different strain hybridization by taking pasture pennisetum as a female parent and long-sequence pennisetum as a male parent to obtain an F1 generation, treating embryo of the F1 generation by adopting a composite biological inducer to generate antioxidant substances, inducing plant cell tissue differentiation, introducing genetic factors, selecting a mutagenized individual from the antioxidant substances, and performing backcross with the male parent long-sequence pennisetum continuously for 4 times to obtain a pennisetum rejuvenation line serving as a male parent of a new pasture grass variety;

step 2: female parent for breeding new forage grass variety

The method comprises the steps of carrying out different strain hybridization by taking grassiness as a female parent and sweet grassiness as a male parent to obtain an F1' generation, adopting a compound biological inducer to treat embryo of the F1 generation, inducing gene mutation, introducing genetic factors, selecting a mutation individual from the embryo, carrying out backcross on the mutation individual with the male parent sweet grassiness continuously by adopting a parent backcross breeding method, and carrying out backcross for 4 times to obtain a grassiness restorer line which is taken as a female parent of a new pasture grass variety;

step 3: breeding of new forage grass variety

And (3) carrying out hybridization on the male parent of the new pasture variety selected in the step (1) and the female parent of the new pasture variety selected in the step (2) to obtain individual pasture variety.

In the breeding process of new forage grass variety, a composite biological inducer is adopted in the process of hybridization of different strain, an ultra-micro speed fusion technology is innovated, antioxidant substances are generated, plant cell tissue differentiation is induced, a large amount of beneficial substances such as amino acids, organic acids, polysaccharides, various vitamins, various biochemical enzymes, growth promoting factors, antioxidant substances, antiviral substances and the like are generated, the immune function of plants is improved, healthy and high-speed growth is promoted, and the obtained hybrid rejuvenation line of different strain and hybrid restoration line of different strain have the advantages of high mating force of 2 parents, stress resistance, strong disease and pest resistance, good tillering property, vigorous growth vigor and the like.

The composite biological inducer has the biggest characteristics of symbiotic co-prosperity, stable state and complete functions, and has the capability of making epoch-making plant individuals. The application innovates an ultra-micro speed fusion technology, induces saccharides generated by photosynthetic bacteria and saccharomycetes to form lactic acid, has strong sterilization capability, maintains ecological balance of a plant body system, and is called as 'post-antibiotic'. It can decompose lignin and cellulose, synthesize various amino acids, vitamins and SOD superoxide dismutase, promote metabolism, dissolve insoluble inorganic phosphorus and maintain plant growth. The photosynthetic bacteria group has photosynthesis and nitrogen fixation, belongs to independent nutrition microorganisms, and can proliferate by itself. The thallus contains more than 60% of protein, is rich in multiple vitamins, contains coenzyme Q10, antiviral substances and growth promoting factors, changes harmful substances into harmless substances, and synthesizes antioxidant substances, saccharides, amino acids, vitamins, nitrogen compounds, antiviral substances, physiologically active substances and the like by taking secretions of plant roots, organic matters in soil, harmful gases (hydrogen sulfide and the like), carbon dioxide, nitrogen and the like as matrixes. It can dissolve insoluble phosphorus and symbiotic with azotobacter to raise its nitrogen fixing capacity.

The application discloses a composite breeding method of pasture, which is characterized in that plant individuals of new varieties of pasture are subjected to asexual propagation and cultivation, and a multi-generation gene optimization technology is carried out, so that genotype phenotype characters of the plant individuals are stable, and new varieties of pasture are obtained.

The application discloses a composite breeding method of pasture, wherein the composite biological inducer is EM original juice.

The application preferably discloses a composite breeding method of pasture, wherein the step 1 also comprises the step of adopting tricyclic alkaloids to treat the embryo of the F1 generation and the embryo of the F1 'generation after adopting a composite biological inducer to treat the embryo of the F1 generation and the embryo of the F1' generation.

Further, the tricyclic alkaloid is carbazole alkaloid Neocarazostatin A or carbazole alkaloid Murrayaquinone A.

The application preferably provides a composite breeding method of pasture, wherein the addition amounts of the composite biological inducer and the tricyclic alkaloid are both 0.5-2.5% of the total weight of embryo.

In the step 3, the hybrid individual gene exhibits partial morphological characters and economic characters which are insufficient, and mutation is induced by irradiation of neodymium glass laser to obtain new pasture variety individuals suitable for breeding targets.

The application preferably discloses a composite breeding method of pasture, wherein the irradiation intensity of neodymium glass laser is 25-29J/square centimeter.

The application relates to a compound breeding method of pasture, which is characterized in that a novel variety of pasture is cultivated by asexual propagation to obtain novel variety of pasture seedlings, and the planting conditions of the novel variety of pasture seedlings are as follows: a) There is no requirement for the soil quality. Suitable for growth under any soil condition. b) The illumination requirement is not high. The sunshine duration is 950 hours or more. c) The water demand is not high. Developed main root and fibrous root, can fully absorb underground water and has strong drought resistance. d) The temperature requirement is not high. High temperature resistance, low temperature resistance and medium-micro frost resistance. e) The organic fertilizer is suitable for application, and the pesticide and fertilizer are not suitable for application. The disease resistance, insect resistance and stress resistance are strong. Plant spacing is 0.5-2.0m, and row spacing is 1.0-5m.

Compared with the prior art, the application has the following advantages and beneficial effects:

1. the application obtains male parent and female parent with strong stress resistance, strong disease and insect resistance, good tillering property and vigorous growth vigor by hybridizing in different strains and adopting alkaloid to induce mutation.

2. The application induces saccharides produced by photosynthetic bacteria and saccharomycetes to form lactic acid through the induction of the compound biological inducer, has strong sterilization capability, maintains ecological balance of plant systems, and is called as 'post-antibiotic'. It can decompose lignin and cellulose, synthesize various amino acids, vitamins and SOD superoxide dismutase, promote metabolism, dissolve insoluble inorganic phosphorus and maintain plant growth. The photosynthetic bacteria group has photosynthesis and nitrogen fixation, belongs to independent nutritive microorganisms, and can proliferate by itself. The thallus contains more than 60% of protein, is rich in multiple vitamins, contains coenzyme Q10, antiviral substances and growth promoting factors, changes harmful substances into harmless substances, and synthesizes antioxidant substances, saccharides, amino acids, vitamins, nitrogen compounds, antiviral substances, physiologically active substances and the like by taking secretions of plant roots, organic matters in soil, harmful gases (hydrogen sulfide and the like), carbon dioxide, nitrogen and the like as matrixes. It can dissolve insoluble phosphorus and symbiotic with azotobacter to raise nitrogen fixing capacity.

Meanwhile, the application also adopts tricyclic alkaloids to induce germs, induces plants to generate the effects of bacteriostasis and disease and insect prevention, and carries out compound induction on germs through EM original juice and tricyclic alkaloids, so that the cultivated pasture has high nutritive value, strong disease and insect resistance and strong environmental stress resistance.

3. The novel pasture of the application has pure ecological characteristics, is not suitable for applying chemical fertilizer, pesticide and herbicide in the planting process, and has strong disease and pest resistance. In the breeding process, antibiotics and hormone are not generally used for all animals, so that the immunity of the animals can be enhanced; the zero emission and zero pollution of the breeding industry are realized, and after all animals eat the breeding industry, the discharged excrement has no foul smell and does not generate flies, mosquitoes and the like; the root system is developed, the fleshy root thoroughly changes the cultivation mode of artificial feed from the source, and the whole ecological industry chain of cultivation is changed into an ecological industry with pure nature, pure ecology, high quality, high safety and low cost in the true sense.

Drawings

The accompanying drawings, which are included to provide a further understanding of embodiments of the application and are incorporated in and constitute a part of this specification, illustrate embodiments of the application and together with the description serve to explain the principles of the application. In the drawings:

FIG. 1 is a diagram of a breeding line of a male parent of a new variety of pasture according to the application.

FIG. 2 is a diagram of a breeding line of a female parent of a new variety of pasture according to the application.

FIG. 3 is a graph comparing the foliar appearance of a new variety of grass of the application with pennisetum hydridum.

FIG. 4 is an external view of a new variety of pasture according to the present application.

FIG. 5 is an external view of a new variety of pasture according to the present application.

FIG. 6 is a plot of the growth of a new variety of pasture according to the application.

FIG. 7 is a graph comparing the stalks of a new variety of grass of the present application with those of pennisetum hydridum.

FIG. 8 is a 10-day growth chart of a new breed of pasture according to the application.

Detailed Description

For the purpose of making apparent the objects, technical solutions and advantages of the present application, the present application will be further described in detail with reference to the following examples and the accompanying drawings, wherein the exemplary embodiments of the present application and the descriptions thereof are for illustrating the present application only and are not to be construed as limiting the present application.

In the present application, HNF14C, GXF, HGF13C, GXX, FJX46, GFX14 represent breeding numbers.

The EM original dew is provided by the developing company of astronomical biotechnology in Jiangxi province.

1. Breeding of new variety of pasture

Example 1

The method comprises the following steps:

step 1: male parent for breeding new variety of pasture

Hybridization is carried out by taking pennisetum mesenchymum HNF14C as female parent and pennisetum longum GXF36 as male parent to obtain F1 generation, and treating the embryo of the F1 generation by adopting EM original dew with embryo quality of 1.6% and carbazole alkaloid Neocarazostatin A with embryo quality of 0.9%, wherein the specific treatment steps are as follows: the seeds of pasture grass F1 are added with water according to the proportion of 1:5, the soaking time is 1 to 2 days, and water is required to be changed for 1 to 2 times or 3 to 4 times. Soaking seeds, airing the seeds at a shade place, covering a moisturizing cloth after the epidermis is air-dried, adopting a reverse osmosis nano film based on a nano film technology when germs sprout, putting the seeds into a thermal insulation and moisturizing (the temperature is 25 ℃ and the humidity is 85% -90%) germination accelerating container, adopting a slow release catheter to reach the germs, adopting a slow release catheter to lead photosynthetic bacteria, saccharomycetes and the like to be subjected to ultraviolet mutagenesis and optimized activation and lead the photosynthetic bacteria, saccharomycetes and the like to be fused, and adopting the slow release catheter to reach the germs by 0.9% of carbazole alkaloid Neocarazostatin A to obtain individuals with good application value.

Through the treatment of EM original dew, antioxidant substances are generated, plant cell tissue differentiation is induced, and genetic factors are introduced, so that the nitrogen fixation capacity of the plant cell tissue differentiation is improved by times. Through the treatment of carbazole alkaloid Neocarazostatin A, disease and pest resistance, bacteriostasis and the like of pasture can be improved, a mutagenized individual is selected from the pasture, the mutagenized individual is continuously backcrossed with the long-sequence pennisetum GXF36 of the male parent, 4 backcrossing is carried out, and the pennisetum rejuvenation line HGF13C is obtained as the male parent of a novel pasture variety GFX14 XHGF 13C, as shown in figure 1;

step 2: female parent for breeding new forage grass variety

The method comprises the steps of taking elephant grass GXX13 as a female parent and sweet elephant grass FJX46 as a male parent to carry out different-line hybridization to obtain F1 generation, adopting an EM original juice with the mass of 1.6% of germ and carbazole alkaloid Neocarazostatin A with the mass of 0.9% to treat the germ of the F1' generation, wherein in the same treatment process as the step 1, the EM original juice is treated to generate antioxidant substances, induce plant cell tissue differentiation and introduce genetic factors, so that the nitrogen fixation capacity of the plant is doubled. Through the treatment of carbazole alkaloid Neocarazostatin A, disease and pest resistance, bacteriostasis and the like of pasture can be improved, induced individuals are selected from the induced individuals, the induced individuals are continuously backcrossed with male parent sweet grassiness FJX46 by adopting a parent backcross breeding method, and the grassiness restorer GFX14 is obtained as a female parent of a new variety of pasture through 4 backcrosses, as shown in figure 2;

step 3: breeding of new forage grass variety

The method is characterized in that a new pasture variety individual is obtained by hybridizing a male parent HGF13C of the new pasture variety bred in the step 1 and a female parent GFX14 of the new pasture variety bred in the step 2, and the new pasture variety individual is irradiated by neodymium glass laser: the leaves become smooth and flat, the growth vigor becomes vigorous, the leaf shape becomes large and long, and after treatment, the new beneficial gene induced mutation characteristic can be caused;

step 4: and (3) obtaining a new pasture variety through asexual propagation cultivation of the new pasture variety individuals obtained in the step (3).

In the step 3, the hybrid individuals are subjected to induced mutation by irradiation of 25-29J/square centimeter neodymium glass laser, and new pasture variety individuals with smooth leaves are obtained.

Cultivation of asexual propagation in areas such as Hainan, sichuan and the like is continuous from 2016-2019, and the characters are stable, so that novel pasture varieties GFX14 XHGF 13C are formed.

Examples 2 to 7

Examples 2-7 differ from example 1 in that the mass ratio of the complex bio-inducer in the germ is 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.2%, 1.4%, 1.6%, 1.8%, 2.0%, 2.2%, 2.5%; the mass ratio of carbazole alkaloid Neocarazostatin A in embryo is 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.2%, 1.4%, 1.6%, 1.8%, 2.0%, 2.2%, 2.5%, and analysis of grass bred in the above examples shows that all of them have gene mutation, which means that the addition of the complex biological inducer and carbazole alkaloid Neocarazostatin A inevitably causes gene mutation, and statistics of mutation probability shows that mutation rate is maximum when the addition amount of carbazole alkaloid Neocarazostatin A is 0.9% at 1.6%.

2. Variety comparison test

In 2017-2018, a variety comparison test is carried out in two consecutive years in a new pasture variety base test of Sichuan Tianfu, the test cell arrangement adopts complete random group arrangement, 3 times of repetition are carried out, the cell area is 4m multiplied by 5m, 6 rows are planted in each cell, the plant spacing is 0.8m, the row spacing is 1m, the control variety is pennisetum hydridum, the tillering, stem thickness, plant height and other agronomic character record is mainly carried out, and 4 times of harvesting are carried out for measuring yield investigation.

3. Variety zoning adaptability test

In 2019-2020, in two years of multi-point regional adaptability tests of Sichuan Luzhou, jintang, guangan, liang mountain Zhou, zhaoju, ganmi Lujing, tibetan Linzhi, chongqing, qianzhou, tianjin Ning river, hainan Zhou, three-year and other places, the pennisetum hydridum is used as a reference, the pennisetum hydridum is arranged in random granules, 3 times of repetition are respectively arranged, each repetition is 1 mu, 1300 plants/mu are planted, 390 plants are surveyed and observed each year, project observation such as plant height, tillering, stem thickness and the like are mainly carried out, and the area of a cell is harvested and the yield per mu is reduced. And analyzing the high yield, stable yield, consistency and specificity.

4. Demonstration test of productivity

In 2019-2020, two years of multi-point production demonstration tests are carried out on adjacent water, parallel-in peace house, luzhou county, jiuzhai ditch in Aba, migo mountain, zhaozhaoju, tibetan Linzhi, chongqing, tongnan, hainan, three years, 3 times of repetition are respectively carried out on pennisetum hydridum serving as a control variety, each repetition is 1 mu, 1300 plants/mu are planted, 390 plants are surveyed and observed each year, the agronomic character records such as tillering, stem thickness, plant height and the like are mainly carried out, and the yield measurement investigation is carried out for 4 times.

5. Statistical treatment of test data

After the test data are processed by excel2007, the statistical software dps v7.55 is used for performing variance analysis on the multi-year multi-point data, and the Duncan method is used for performing multiple comparison.

6. Test results and analysis

6.1 morphological characterization

The novel forage grass variety GFX14 XHGF 13C has high growth speed and high yield. The general annual growth is 7-9 m high, the tillers are 42-91, the stem thickness can reach 3.5-4.1 cm, the internode length is 7-15 cm, each node is wrapped by blades, the blades grow mutually, the length is 81-122 cm, the blade width is 4-6 cm, and the plant is free of burrs and saw teeth, as shown in figure 3; she Kuanshe is long and fresh, and can reduce intestinal tract disease of livestock, as shown in figure 4, and leaves are waxy, glossy and free of villus, as shown in figure 5. Dense conical inflorescences are 20-30 cm long. Under the agro-farming climate conditions suitable for the growth in the west, middle and south, the annual yield of fresh grass is 30-40 t/mu, and some fresh grass is as high as 60 t/mu. While the pennisetum hydridum has burrs and saw teeth, and when animals such as sheep eat the pennisetum hydridum, lips are easy to cut off blood

The grass features high plant height up to 8-10 m, strong tillering and regeneration capacity, drought resistance and certain salt tolerance and moisture resistance. The average daily length of the grass is 8-13 cm, the weight of one grass reaches 2-3 kg, the grass growing capacity of one fresh grass bud is increased in geometric multiple, the grass can grow into a forest (shed), more than 100 plants can be grown, and the good reach of 200 plants is shown in figure 6. Each root of the grass can reach hundreds of jin, and the root system is particularly developed.

The variety-specific trait stems are solid, and the obvious distinguishing features of the variety-specific trait stems and the pennisetum hydridum stems are shown in fig. 7.

6.2 biological Properties

Novel grass species GFX14×hgf13c genus angiosperms phylum, monocotyledonous class, poaceae family, genus pennisetum. The method of harvesting for many years can be adopted in south of China, higher yield can be obtained, and the yield per mu is higher in suitable soil environment, air temperature and other factors. The growth starts under the air temperature condition of 12 ℃ to 15 ℃, the temperature of 25 ℃ to 35 ℃ is proper, the growth is inhibited below 10 ℃, and the growth is stopped below 5 ℃. Symbiotic with azotobacter can increase nitrogen fixation ability. The new variety GFX14 XHGF 13C can be planted in most areas of the country as shown in FIG. 6.

As high-quality forage grass, the forage grass can replace silage corn and grain, and provides high-quality ecological forage grass with good palatability for herbivores such as cattle, sheep, pigs, horses, donkeys, rabbits, chickens, ducks, geese, fishes and the like, thereby greatly relieving the contradiction between serious shortage of green forage grass and human and animal food.

6.3 high photosynthetic Rate

The initial product of the photosynthesis of the novel forage grass variety GFX14 XHGF 13C is 4-carbonic acid-hydrocarbon succinic acid, aspartic acid and other four-carbon dicarboxylic acid products, namely the photosynthesis biochemical path is C-4, belongs to typical four-carbon plants, and has higher photosynthesis rate. The photosynthetic rate was determined to be 50-70 mg CO 2/dm 2/hr. This characteristic is a decisive factor for its high yield as shown in fig. 8.

6.4 yield investigation

6.4.1 variety comparison test

The pasture variety base test in the new area of Sichuan Tianfu in 2017-2018 is carried out for two consecutive years, and is managed in a non-fertilizing mode, and test results show that the yield of the novel pasture variety GFX14 XHGF 13C in 2017-2018 is obviously higher than that of the control pennisetum hydridum (P is less than 0.01) as shown in table 1. Wherein, in 2017 variety comparison test, the average acre yield of fresh grass is 27.00t, the 1 st place is higher than that of control pennisetum hydridum, and the yield is increased by 11.11%; in 2018, a variety comparison test is carried out, the average acre yield of the fresh grass is 41.50t, the 1 st place is occupied, and the yield is increased by 12.05% compared with the yield of the control pennisetum hydridum; the average acre yield of the fresh grass in two years is 34.25t, and the yield is increased by 11.71 percent compared with the control.

TABLE 1 grass yield of novel grass variety GFX14 XHGF 13C comparative test

Remarks: the control seed is pennisetum hydridum.

6.4.2 variety zoning adaptability test

The adaptability test for the variety zoning was carried out in two consecutive years from 2019 to 2020, and the results are shown in Table 2. At 12 test points of Sichuan Luzhou, jintang, guangan, liangshan Zhou Mei Gu, zhaoju, ganzhu Linzhi, chongqing Tongnan, wanzhou, tianjin Ning river, hainan Yangzhou, sanand the like, the average fresh grass mu yield of the new forage grass variety GFX14×HGF13C is 43.00t for two years, and is increased by 17.68 percent compared with the control pennisetum hydridum, thereby reaching extremely remarkable level. Wherein, in 2019 variety regionalization adaptability test, the average acre yield of the test seed fresh grass is 38.99t, which is increased by 17.90 percent compared with 33.07t of control pennisetum hydridum; in the 2020 variety zoning adaptability test, the average acre yield of the test seed fresh grass is 47.00t, which is increased by 17.50% compared with 40.00t of the control pennisetum hydridum; the average acre fresh grass yield of 43.00t in two years is increased by 17.68 percent compared with the control pennisetum hydridum.

The analysis of the high yield and stable yield of the novel forage grass variety GFX14 XHGF 13C is shown in Table 3, and the novel forage grass variety GFX14 XHGF 13C and the novel control variety pennisetum hydridum are suitable for being planted at 12 test points, and the high yield and stable yield of the novel forage grass variety GFX14 XHGF 13C are obviously better than those of the novel control variety pennisetum hydridum.

TABLE 2 investigation and analysis of yield of GFX14 XHGF 13C regionalization adaptability test for novel pasture variety

Note that: the control seed is pennisetum hydridum.

TABLE 3 analysis of high yield and stability of novel forage GFX14 XHGF 13C regionalized adaptability test

Note that: E1-Sichuan Luzhou; E2-Sichuan Jintang; E3-Sichuan Guangan; E4-Liangshan Zhou Mei Gu; E5-Zhaoshan Zhaozhuo; E6-Ganzhou lujing; E7-Tibetan Linzhi; E8-Chongqing Tongnan; E9-Chongqing Wanzhou; E10-Tianjin Ning river; e11-hainan, state; e12 Hainan three-way.

6.4.3 production demonstration test investigation analysis

In 2019-2020, on the basis of a variety comparison test, two-year multi-point production demonstration test surveys are conducted at 10 test points of Sichuan adjacent water, parallel peace, lu county, jiuzhai in Aban, meailou in Liangshan, zhaojuan, tibetan Linzhi, chongqing, tongnan, hainan, three-year and the like. The new forage grass variety GFX14 XHGF 13C has vigorous growth and strong adaptability, the plant height after harvesting for 5-6 weeks can reach more than 1.5-2 m, the forage grass novel forage grass variety shows excellent production performance, the average acre yield of fresh grass for two years at 10 test points is 43.41t, the forage grass novel forage grass variety is 18.64 percent of the forage grass yield is increased compared with the contrast seed, and the analysis of variance result shows that the forage grass novel forage grass variety is extremely obviously higher than the contrast seed (P < 0.01) as shown in a table 4.

TABLE 4 analysis of production test yield of novel grass variety GFX14 XHGF 13C

Note that: the control variety is pennisetum hydridum.

6.5 detection and analysis of nutritional ingredients

The nutrient content detection data are from a third party detection mechanism, and the result of the zoning adaptability test is shown in table 5, and the nutrient content of the novel forage grass variety GFX14×HGF13C is high. The index of each nutrition ingredient tested by the analysis test center of the academy of agricultural science of Sichuan province is shown in table 5:

TABLE 5 GFX14 XHGF 13C nutritional ingredients of novel pasture variety

Whereas the control group of pennisetum hydridum has the following nutritional ingredients:

11.6% of crude protein, 1.74% of crude fat and 22.45% of nitrogen-free extract.

Therefore, the nutritional value of the novel forage grass variety is obviously higher than that of pennisetum hydridum.

Discussion and analysis 7

The novel forage grass variety GFX14 XHGF 13C is used for feeding herbivorous domestic animals and poultry such as cattle, sheep, pigs, chickens, ducks, geese, fishes, rabbits and the like, can replace the traditional formula compound feed and a large amount of grains, improves the quality of the livestock products, and greatly reduces the feed cost. Tests prove that the quality of the forage grass is high-yield, high-protein, high-fat and high-quality forage grass.

7.1 area or Environment of growth

The new forage grass variety GFX14 XHGF 13C can be planted in the south all the year round, and is suitable for planting in the north from the bottom of 4 months to 7 months in spring and at the temperature of above 12 ℃. Through the breeding of years and the trial demonstration of each test point, the novel forage grass variety GFX14 XHGF 13C shows excellent characteristics, and has the advantages of high plant height, upright cluster, developed root system, wide adaptability, strong stress resistance, high yield, higher crude protein and other nutrient contents, and is suitable for planting in most areas of China.

7.2 simplification of cultivation technique

The novel forage grass variety GFX14 XHGF 13C is drought-resistant and barren-resistant, can be planted in barren slopes, river sides, reservoir sides, farmland sides and front and rear houses, and can be planted in scattered places, if soil layers are used as forage grass, the forage grass should be rich, and the soil with sufficient water sources is favorable for exerting the high yield characteristics of the novel forage grass variety GFX14 XHGF 13C. If the yield is not pursued on one side, the method can adopt extensive planting management as desertification control.

7.3 planting management ecology

The novel forage grass variety GFX14 XHGF 13C is simple in planting management technology. Weeding is carried out for 1 time after germination, and additional fertilizer is applied after each harvesting. From the experimental and experimental demonstration conditions, the novel forage grass variety GFX14 XHGF 13C can be grown in the same vigorous manner without using pesticides and chemical fertilizers and without using herbicides, and if the chemical fertilizer is used improperly, fertilizer damage can be caused, and normal growth is affected.

7.4 variety specificity, identity and stability

7.4.1 specificity analysis

The special stems of the novel forage grass variety GFX14 XHGF 13C are solid, while the stems of the control variety pennisetum hydridum are hollow; the leaf margin of the novel forage grass variety GFX14 XHGF 13C is smooth and has no saw teeth; the leaf edge of the pennisetum hydridum is provided with small saw teeth; leaves of the new grass variety GFX14 XHGF 13C are waxy and glossy; pennisetum hydridum leaf mask short villus.

7.4.2 consistency analysis

The novel forage grass variety GFX14 XHGF 13C has the same character and consistency in the planting area of tens of thousands of acres in Hainan and Sichuan, and the novel forage grass variety GFX14 XHGF 13C has the same consistency.

7.4.3 stability analysis

In 2017-2020, continuously planting for 4 years in Tianfu new areas of Sichuan province, taking a new forage grass variety GFX14×HGF13C as a test variety, taking pennisetum hydridum as a control variety, performing growth expression and yield distribution test together, setting 3 times of repetition, 1 mu of each repetition, planting 1300 plants/mu, examining and observing 390 plants each year, wherein the new forage grass variety GFX14×HGF13C has high purity, consistent phenotype and stable generation-to-generation.

Conclusion 8

The novel forage grass variety GFX14 XHGF 13C is perennial gramineous forage grass, has high plant size, upright clusters, developed root system, certain drought resistance, stress resistance, high yield and wide adaptability, and has the advantages of strong plant regeneration capability after harvesting, high growth speed, strong disease and insect resistance, good tillering performance, high yield, high nutritional value and good palatability. The variety comparison test, the regionalization adaptability test and the productivity demonstration test show that the novel forage grass variety GFX14×HGF13C is extremely superior to the control pennisetum hydridum in terms of acre yield, quality and the like, is excellent in 12 test points for carrying out the variety regionalization adaptability test, is obviously superior to the control pennisetum hydridum in terms of high yield and stable yield, and is suitable for being planted and popularized and applied in vast majority of national provincial areas such as Sichuan, guangdong, guangxi, hainan, chongqing, tianjin, tibet and the like.

The foregoing detailed description of the preferred embodiments has been provided for the purpose of illustrating the general principles of the application, and is not meant to limit the scope of the application, but to limit the application to the particular embodiments disclosed.

Claims

1. A composite breeding method of pasture, which is characterized by comprising the following steps:

step 1: male parent for breeding new variety of pasture

Performing different strain hybridization by taking pasture pennisetum as a female parent and long-sequence pennisetum as a male parent to obtain an F1 generation, treating the embryo of the F1 generation by adopting an EM original juice with the mass of 1.4% -1.8% and carbazole alkaloid Neocarazostatin A with the mass of 0.7% -1.2%, inducing plant cell tissue differentiation, inducing gene mutation, selecting a mutagenized individual, and then carrying out backcross with the male parent long-sequence pennisetum continuously, and carrying out backcross for a plurality of times to obtain a pennisetum rejuvenation line as the male parent of a new pasture grass variety;

step 2: female parent for breeding new forage grass variety

The method comprises the steps of carrying out different strain hybridization by taking grassiness as a female parent and sweet grassiness as a male parent to obtain an F1 'generation, adopting an EM original dew with the germ mass of 1.4% -1.8% and carbazole alkaloid Neocarazostatin A with the germ mass of 0.7% -1.2% to treat the germ of the F1' generation, inducing gene mutation, selecting a mutagenized individual from the germ, carrying out parental backcross breeding on the mutagenized individual, continuously backcrossing with the male parent sweet grassiness, and carrying out backcross for multiple times to obtain a grassiness restorer line which is used as a female parent of a new grassiness variety;

step 3: breeding of new forage grass variety

And (3) hybridizing the male parent of the new pasture variety bred in the step (1) with the female parent of the new pasture variety bred in the step (2) to obtain an individual of the new pasture variety.

2. The method for composite breeding of pasture according to claim 1, wherein the individual of the new variety of pasture is subjected to asexual propagation cultivation, and a multi-generation gene optimization technology is performed to stabilize the genotype phenotype character of the individual to obtain the new variety of pasture.

3. The composite breeding method of pasture according to claim 1 or 2, wherein the individual of the new pasture variety is subjected to asexual propagation and cultivation to obtain the young pasture variety seedling, and the planting conditions of the young pasture variety seedling are as follows: the plant spacing is 0.5-2.0m, and the row spacing is 1-5 m.

4. A method of composite breeding of pasture according to claim 1 or 2, characterized in that the number of backcrosses is 4.