CN114736777A - Special ultrafiltration of medicine carrying exosome extraction and separation, super integrative device that leaves - Google Patents

Abstract

The invention discloses a special ultrafiltration and superseparation integrated device for extracting and separating drug-loaded exosomes, which comprises a workbench, wherein one side of the top end of the workbench is fixedly connected with a fixed cylinder through a fixed plate, the inner side of the fixed cylinder is fixedly connected with a connecting seat, the outer side of the top end of the connecting seat is fixedly connected with an outer ring, and the top end of the connecting seat is positioned on the inner side of the outer ring and is fixedly connected with an inner ring; through setting up centrifuge bowl and ultrafiltration jar, to inside appropriate amount of extract and the buffer of adding of centrifuge bowl, start the motor of connecting plate bottom, the output that makes the motor drives the centrifuge bowl through the link and carries out centrifugal motion, and then the extract to centrifuge bowl inside is centrifuged, make the inside exosome supernatant of centrifuge bowl inside discharge the inside of funnel through the supernatant export after the centrifugation, then the inside that gets into the ultrafiltration jar through the connecting pipe of funnel bottom is ultrafiltered, and then the effect of centrifugation and filtration integration has been reached, and the pollution to exosome has been reduced.

Description

Special ultrafiltration of medicine carrying exosome extraction and separation, super integrative device that leaves

Technical Field

The invention belongs to the technical field of exosome extraction and separation, and particularly relates to an ultrafiltration and superseparation integrated device special for medicine-carrying exosome extraction and separation.

Background

Exosomes refer to small membrane vesicles (30-150nm) containing complex RNAs and proteins, which today refer specifically to discoid vesicles with diameters between 40-100 nm; many cells secrete exosomes under both normal and pathological conditions. The liposome is mainly derived from a multivesicular body formed by invagination of intracellular lysosome particles, and is released into extracellular matrix after fusion of an outer membrane of the multivesicular body and a cell membrane; all cultured cell types secrete exosomes and exosomes are naturally present in body fluids, including blood, saliva, urine, cerebrospinal fluid and milk; the precise molecular mechanisms involved in their secretion and uptake and their composition, "cargo" and corresponding functions have just begun to be studied; exosomes are considered as specifically secreted membrane vesicles, involved in intercellular communication, and interest in exosome research is growing, whether to study their function or to understand how to use them for the development of minimally invasive diagnostics.

At present when extracting exosome, centrifugation and filtration process need go on alone, and then lead to extraction efficiency lower to make exosome receive the pollution easily, consequently provide a special ultrafiltration of medicine carrying exosome extraction separation, super from integrative device.

Disclosure of Invention

The invention aims to overcome the defects in the prior art and provides an ultrafiltration and super-separation integrated device special for extracting and separating drug-loaded exosomes, so as to solve the problems that the extraction efficiency is low and exosomes are easily polluted in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme: an ultrafiltration and super-separation integrated device special for extracting and separating drug-loaded exosomes comprises a workbench, wherein one side of the top end of the workbench is fixedly connected with a fixed cylinder through a fixed plate, the inner side of the fixed cylinder is fixedly connected with a connecting seat, the outer side of the top end of the connecting seat is fixedly connected with an outer ring, the top end of the connecting seat is positioned on an inner side fixedly connected with an inner ring of the outer ring, the top end of the inner ring is fixedly connected with a connecting plate, the bottom end of the connecting plate is fixedly connected with a motor, the output end of the motor is fixedly connected with a connecting frame, the other end of the connecting frame is fixedly connected with a centrifugal tank, the outer side of the upper end of the centrifugal tank is fixedly connected with a supernatant outlet, the bottom end of the centrifugal tank is fixedly connected with a lower-layer liquid outlet, the outer sides of the supernatant outlet and the lower-layer liquid outlet are respectively provided with a first electromagnetic valve and a second electromagnetic valve, the outer side of the upper end of the fixed cylinder is fixedly connected with a funnel, the funnel is located the below of supernatant export, the bottom fixedly connected with connecting pipe of funnel, the connecting pipe other end fixedly connected with ultrafiltration jar, ultrafiltration jar fixed connection is in the top of workstation.

Preferably, be provided with solenoid valve three between ultrafiltration jar and the connecting pipe, the bottom of ultrafiltration jar is provided with solenoid valve four, the outside of ultrafiltration jar is provided with negative pressure device, ultrafiltration jar top is provided with the sealed lid of ultrafiltration, the inside upper end of ultrafiltration jar is provided with the milipore filter.

Preferably, a first conductive sliding ring is arranged on the outer side of the supernatant outlet, a first conductive sliding ring is arranged on the outer side of the subnatant outlet, the first conductive sliding ring is positioned at the top end of the electrifying torch, the first conductive sliding ring and an inner ring of the first conductive sliding ring are respectively electrically connected with a first electromagnetic valve and a second electromagnetic valve, and the electrifying torch and the electrifying ring are both electrically connected with a power supply.

Preferably, the outside fixedly connected with neck cover of solid fixed cylinder upper end, the neck cover is located the outside of funnel, the neck cover upper end is provided with the spacing groove, there is the protective cover neck cover top through the spacing groove joint, the protective cover top corresponds the centrifuge bowl has seted up the opening.

Preferably, one side fixedly connected with support on workstation top, support one side is provided with buffer solution storage jar, the bottom fixedly connected with hose of buffer solution storage jar, hose other end fixedly connected with liquid feeding rifle, the liquid feeding rifle block is in the outside of support.

Preferably, the top end of the centrifugal tank is provided with a super-separation sealing cover, the top end of the super-separation sealing cover is fixedly connected with a liquid inlet pipe, the inner side of the top end of the liquid inlet pipe is provided with a leather plug, and the liquid inlet pipe is positioned right below the opening.

Preferably, the bottom end of the fixed cylinder is fixedly connected with a liquid outlet hopper, the bottom end of the liquid outlet hopper penetrates through the workbench and is located inside the drawer, and a waste liquid box is arranged inside the drawer.

Preferably, the top end of the ultrafiltration tank penetrates through the workbench and is positioned inside the drawer, and the bottom ends of the ultrafiltration tank and the liquid outlet hopper are both positioned above the waste liquid box.

Preferably, a control panel is arranged on the outer side of the support and is electrically connected with the motor, the first electromagnetic valve, the second electromagnetic valve, the third electromagnetic valve, the negative pressure device and the fourth electromagnetic valve.

Preferably, the inner side of the limit groove is provided with a contact protection switch, and the contact protection switch is electrically connected with the control panel.

Compared with the prior art, the invention provides a special ultrafiltration and superseparation integrated device for extracting and separating drug-loaded exosomes, which has the following beneficial effects:

1. according to the invention, the centrifugal tank and the ultrafiltration tank are arranged, a proper amount of extracting solution and buffer solution are added into the centrifugal tank, the motor at the bottom end of the connecting plate is started, the output end of the motor drives the centrifugal tank to do centrifugal motion through the connecting frame, then the extracting solution in the centrifugal tank is centrifuged, after centrifugation, the supernatant of exosomes in the centrifugal tank is discharged into the funnel through the supernatant outlet, and then enters the ultrafiltration tank through the connecting pipe at the bottom end of the funnel for ultrafiltration, so that the effect of integration of centrifugation and filtration is achieved, and the pollution to exosomes is reduced;

2. according to the invention, the super-centrifugal sealing cover is arranged, the top end of the super-centrifugal sealing cover is fixedly connected with the liquid inlet pipe, the super-centrifugal sealing cover is covered on the top end of the centrifugal tank, the centrifugal tank is stopped at the original point by the motor after a certain time, the extracting solution in the centrifugal tank is layered after standing for a period of time, other impurities in the extracting solution are located in the lower layer liquid, the leather plug at the top end of the liquid inlet pipe is detached after the lower layer liquid is discharged, then a proper amount of buffer solution is injected into the liquid inlet pipe through the liquid adding gun, the liquid adding gun is communicated with the buffer solution storage tank through the hose, so that the buffer solution is continuously added into the centrifugal tank, and the extraction purity of exosomes is improved;

3. according to the invention, through arranging the electric torch and the electric ring, in the centrifugation process, the first conductive sliding ring positioned at the outer side of the supernatant outlet rolls at the top end of the electric torch, the second conductive sliding ring positioned at the outer side of the subnatant outlet rolls at the inner side of the electric ring, the electric torch and the electric ring are electrically connected with a power supply, the first conductive sliding ring and the inner ring of the first conductive sliding ring are respectively electrically connected with the first electromagnetic valve and the second electromagnetic valve, and the first electromagnetic valve and the second electromagnetic valve are respectively connected into a circuit under the action of the first conductive sliding ring and the outer ring of the first conductive sliding ring and can continuously rotate, so that the first electromagnetic valve and the second electromagnetic valve can be conveniently controlled at any time.

The device has the advantages that the structure is scientific and reasonable, the use is safe and convenient, and great help is provided for people.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention without limiting the invention in which:

fig. 1 is an isometric structural schematic diagram of an ultrafiltration and superseparation integrated device special for extracting and separating drug-loaded exosomes, provided by the invention;

fig. 2 is an explosion structure schematic diagram of an ultrafiltration and superseparation integrated device special for extracting and separating a drug-loaded exosome provided by the invention;

fig. 3 is a schematic structural view of a main view of an ultrafiltration and superseparation integrated device special for extracting and separating a drug-loaded exosome provided by the invention;

fig. 4 is a schematic side view of an ultrafiltration and superseparation integrated device special for extracting and separating a drug-loaded exosome according to the present invention;

FIG. 5 is a schematic cross-sectional view taken at A-A in FIG. 4;

FIG. 6 is a schematic structural diagram of a power-on torch and a power-on loop in the ultrafiltration and superseparation integrated device special for extracting and separating drug-loaded exosomes provided by the invention;

fig. 7 is a schematic diagram of a partial explosion structure of an ultrafiltration and superseparation integrated device special for extracting and separating a drug-loaded exosome provided by the invention;

FIG. 8 is a schematic structural diagram of an ultrafiltration tank in the special ultrafiltration and superseparation integrated device for extracting and separating drug-loaded exosomes provided by the invention;

in the figure: the device comprises a workbench 1, a fixing plate 2, a fixing barrel 3, a connecting seat 4, an outer ring 5, an inner ring 6, a connecting plate 7, a motor 8, a connecting frame 9, a centrifugal tank 10, a supernatant outlet 11, a lower layer liquid outlet 12, a first electromagnetic valve 13, a second electromagnetic valve 14, a funnel 15, a connecting pipe 16, an ultrafiltration tank 17, a third electromagnetic valve 18, a negative pressure device 19, a fourth electromagnetic valve 20, an ultrafiltration sealing cover 21, a first conductive sliding ring 22, a current-carrying barrel 23, a conductive sliding ring 24, a current-carrying ring 25, a neck sleeve 26, a limiting groove 27, a protective cover 28, an opening 29, a support 30, a buffer storage tank 31, a hose 32, a liquid feeding gun 33, an ultrafiltration sealing cover 34, a liquid inlet pipe 35, a leather plug 36, a liquid outlet hopper 37, a drawer 38, a waste liquid box 39 and a control panel 40.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-8, the present invention provides a technical solution: an ultrafiltration and super-separation integrated device special for extracting and separating drug-loaded exosomes comprises a workbench 1, wherein one side of the top end of the workbench 1 is fixedly connected with a fixed cylinder 3 through a fixed plate 2, the inner side of the fixed cylinder 3 is fixedly connected with a connecting seat 4, the outer side of the top end of the connecting seat 4 is fixedly connected with an outer ring 5, the top end of the connecting seat 4 is fixedly connected with an inner ring 6 at the inner side of the outer ring 5, the top end of the inner ring 6 is fixedly connected with a connecting plate 7, the bottom end of the connecting plate 7 is fixedly connected with a motor 8, the output end of the motor 8 is fixedly connected with a connecting frame 9, the other end of the connecting frame 9 is fixedly connected with a centrifugal tank 10, the outer side of the upper end of the centrifugal tank 10 is fixedly connected with a supernatant outlet 11, the bottom end of the centrifugal tank 10 is fixedly connected with a lower liquid outlet 12, the outer sides of the supernatant outlet 11 and the lower liquid outlet 12 are respectively provided with a first electromagnetic valve 13 and a second electromagnetic valve 14, and the outer side of the upper end of the fixed cylinder 3 is fixedly connected with a funnel 15, funnel 15 is located the below of supernatant export 11, the bottom fixedly connected with connecting pipe 16 of funnel 15, connecting pipe 16 other end fixedly connected with ultrafiltration jar 17, ultrafiltration jar 17 fixed connection is on the top of workstation 1, to the inside appropriate amount of extract and buffer of adding of centrifugation jar 10, start the motor 8 of connecting plate 7 bottom, the output that makes motor 8 drives centrifugation jar 10 through link 9 and carries out centrifugal motion, and then centrifuge the inside extract of centrifugation jar 10, make the inside exosome supernatant of centrifugation jar 10 discharge the inside of funnel 15 through supernatant export 11 after the centrifugation, then 16 inside that get into ultrafiltration jar 17 of connecting pipe through funnel 15 bottom are ultrafiltered, and then the effect of centrifugation and filtration integration has been reached, and the pollution to exosome has been reduced.

In the present invention, preferably, a third electromagnetic valve 18 is disposed between the ultrafiltration tank 17 and the connection pipe 16, a fourth electromagnetic valve 20 is disposed at the bottom end of the ultrafiltration tank 17, a negative pressure device 19 is disposed at the outer side of the ultrafiltration tank 17, an ultrafiltration sealing cover 21 is disposed at the top end of the ultrafiltration tank 17, an ultrafiltration membrane is disposed at the upper end inside the ultrafiltration tank 17, the ultrafiltration tank 17 is sealed by the third electromagnetic valve 18 and the fourth electromagnetic valve 20 after the supernatant enters, and then the negative pressure device 19 generates a negative pressure inside the ultrafiltration tank 17, so that the supernatant is separated by the ultrafiltration membrane inside the ultrafiltration tank 17.

In the invention, preferably, a first conductive slip ring 22 is arranged outside the supernatant outlet 11, a second conductive slip ring 24 is arranged outside the subnatant outlet 12, the first conductive slip ring 22 is positioned at the top end of the electrifying cylinder 23, the first conductive slip ring 24 is positioned inside the electrifying ring 25, the first conductive slip ring 22 and the inner ring of the first conductive slip ring 24 are respectively and electrically connected with the first electromagnetic valve 13 and the second electromagnetic valve 14, the electrifying cylinder 23 and the electrifying ring 25 are both electrically connected with a power supply, during the centrifugation process, the first conductive slip ring 22 positioned outside the supernatant outlet 11 rolls at the top end of the electrifying cylinder 23, the second conductive slip ring 24 positioned outside the subnatant outlet 12 rolls inside the electrifying ring 25, the electrifying cylinder 23 and the electrifying ring 25 are electrically connected with the power supply, and the inner rings of the first conductive slip ring 22 and the second conductive slip ring 24 are respectively and electrically connected with the first electromagnetic valve 13 and the second electromagnetic valve 14, so as to respectively connect the first electromagnetic valve 13 and the second electromagnetic valve 14 into the circuit under the action of the first conductive slip ring 22 and the outer ring 24, and can rotate continuously, and then be convenient for control solenoid valve 13 and solenoid valve two 14 at any time.

In the invention, preferably, the outer side of the upper end of the fixing cylinder 3 is fixedly connected with a neck sleeve 26, the neck sleeve 26 is positioned on the outer side of the funnel 15, the upper end of the neck sleeve 26 is provided with a limiting groove 27, the top end of the neck sleeve 26 is clamped with a protective cover 28 through the limiting groove 27, the top end of the protective cover 28 is provided with an opening 29 corresponding to the centrifugal tank 10, the upper end of the neck sleeve 26 is provided with the limiting groove 27 corresponding to the protective cover 28, so that the protective cover 28 and the neck sleeve 26 can be assembled conveniently, and the opening 29 at the top end of the protective cover 28 is positioned right above the centrifugal tank 10.

In the present invention, preferably, one side of the top end of the workbench 1 is fixedly connected with a support 30, one side of the support 30 is provided with a buffer storage tank 31, the bottom end of the buffer storage tank 31 is fixedly connected with a hose 32, the other end of the hose 32 is fixedly connected with a liquid adding gun 33, the liquid adding gun 33 is clamped on the outer side of the support 30, the liquid adding gun 33 is communicated with the buffer storage tank 31 through the hose 32, and the buffer storage tank 31 is arranged on the outer side of the support 30, so as to facilitate the continuous addition of buffer liquid into the centrifuge tank 10, and improve the extraction purity of exosomes.

In the invention, preferably, the top end of the centrifugal tank 10 is provided with a super-centrifugal sealing cover 34, the top end of the super-centrifugal sealing cover 34 is fixedly connected with a liquid inlet pipe 35, the inner side of the top end of the liquid inlet pipe 35 is provided with a leather plug 36, the liquid inlet pipe 35 is positioned right below the opening 29, and as the centrifugal tank 10 is positioned at an original point, the liquid inlet pipe 35 at the top end of the centrifugal tank 10 is positioned right below the opening 29, so that the leather plug 36 at the top end of the liquid inlet pipe 35 can be conveniently detached through the opening 29, and then a proper amount of buffer solution is injected into the liquid inlet pipe 35 through the liquid feeding gun 33.

In the present invention, preferably, the bottom end of the fixed cylinder 3 is fixedly connected with a liquid outlet funnel 37, the bottom end of the liquid outlet funnel 37 penetrates through the workbench 1 and is located inside the drawer 38, a waste liquid box 39 is arranged inside the drawer 38, and the discharged waste liquid enters the inner side of the liquid outlet funnel 37 through the lower layer liquid outlet 12, then is discharged into the drawer 38 through the liquid outlet funnel 37, and finally is collected through the drawer 38.

In the invention, preferably, the top end of the ultrafiltration tank 17 penetrates through the workbench 1 and is positioned inside the drawer 38, the bottom ends of the ultrafiltration tank 17 and the liquid outlet hopper 37 are both positioned above the waste liquid box 39, the electromagnetic valve four 20 is opened, so that the filtered waste liquid enters the drawer 38 through the electromagnetic valve four 20, and the waste liquid box 39 is arranged inside the drawer 38 and below the electromagnetic valve four 20 and the liquid outlet hopper 37, thereby facilitating the centralized treatment of the waste liquid.

In the present invention, preferably, a control panel 40 is disposed outside the bracket 30, the control panel 40 is electrically connected to the motor 8, the first electromagnetic valve 13, the second electromagnetic valve 14, the third electromagnetic valve 18, the negative pressure device 19, and the fourth electromagnetic valve 20, and the motor 8, the first electromagnetic valve 13, the second electromagnetic valve 14, the third electromagnetic valve 18, the negative pressure device 19, and the fourth electromagnetic valve 20 are controlled by the control panel 40 according to a set instruction to operate, so as to facilitate automatic extraction.

In the present invention, preferably, a contact protection switch is disposed inside the limiting groove 27, and the contact protection switch is electrically connected to the control panel 40, and when the protective cover 28 is in place, the contact protection switch can be triggered, and the control panel 40 can only perform the extraction operation when receiving an electrical signal from the contact protection switch, otherwise, the extraction operation cannot be performed, so as to improve the safety of use.

The working principle and the using process of the invention are as follows: when the centrifugal liquid extracting device is used, a proper amount of extracting liquid and buffer liquid are added into the centrifugal tank 10, the super-centrifugal sealing cover 34 is covered at the top end of the centrifugal tank 10, then the protective cover 28 is covered at the top end of the neck sleeve 26, the upper end of the neck sleeve 26 is provided with the limiting groove 27 corresponding to the protective cover 28, the protective cover 28 and the neck sleeve 26 are convenient to assemble, an opening 29 at the top end of the protective cover 28 is positioned right above the centrifugal tank 10, then the rotating speed and time of the motor 8 are set through the control panel 40, the motor 8 at the bottom end of the connecting plate 7 is started, the output end of the motor 8 drives the centrifugal tank 10 to carry out centrifugal motion through the connecting frame 9, further, the extracting liquid in the centrifugal tank 10 is centrifuged, in the centrifugal process, the conductive sliding ring I22 positioned outside the supernatant outlet 11 rolls at the top end of the electrifying cylinder 23, and the conductive sliding ring I24 positioned outside the lower liquid outlet 12 rolls at the inner side of the liquid inlet pipe 35, the electric torch 23 and the electrifying ring 25 are electrically connected with a power supply, the inner rings of the conductive slip ring I22 and the conductive slip ring 24 are respectively electrically connected with the electromagnetic valve I13 and the electromagnetic valve II 14, the electromagnetic valve I13 and the electromagnetic valve II 14 are respectively connected into a circuit under the action of the outer ring of the conductive slip ring I22 and the conductive slip ring 24 and can continuously rotate, so that the electromagnetic valve I13 and the electromagnetic valve II 14 can be conveniently controlled at any time, the motor 8 stops the centrifugal tank 10 at the original point after a certain time, the extracting solution in the centrifugal tank 10 is layered after standing for a period of time, other impurities in the extracting solution are positioned in the lower layer liquid, then the electromagnetic valve II 14 positioned outside the lower layer liquid outlet 12 at the bottom end of the centrifugal tank 10 is controlled to be opened through the control panel 40, the lower layer liquid in the centrifugal tank 10 is discharged, and the discharged waste liquid enters the inner side of the liquid outlet hopper 37 through the lower layer liquid outlet 12, then discharged into the drawer 38 through the liquid outlet funnel 37, because the centrifuge tank 10 is located at the original point, the liquid inlet pipe 35 at the top end of the centrifuge tank 10 is located right below the opening 29, and then the leather plug 36 at the top end of the liquid inlet pipe 35 is conveniently detached through the opening 29, then a proper amount of buffer solution is injected into the liquid inlet pipe 35 through the liquid adding gun 33, the liquid adding gun 33 is communicated with the buffer solution storage tank 31 through the hose 32, the buffer solution storage tank 31 is arranged at the outer side of the bracket 30, so that the buffer solution is continuously added into the centrifuge tank 10, the extraction purity of exosomes is improved, after repeated centrifugation, the electromagnetic valve 13 at the outer side of the supernatant outlet 11 is controlled to be opened through the control panel 40, the supernatant of exosomes in the centrifuge tank 10 is discharged into the funnel 15 through the supernatant outlet 11, and then enters the interior of the ultrafiltration tank 17 through the connecting pipe 16 at the bottom end of the funnel 15, after the supernatant fluid gets into, seal ultrafiltration jar 17 through three 18 of solenoid valves and four 20 of solenoid valves, then make the inside negative pressure that produces of ultrafiltration jar 17 through negative pressure device 19, and then make the supernatant fluid separate through the inside milipore filter of ultrafiltration jar 17, then open four 20 of solenoid valves, make filterable waste liquid get into the inside of drawer 38 through four 20 of solenoid valves, the inside of drawer 38 is located four 20 of solenoid valves and is provided with waste liquid box 39 with the below of going out liquid fill 37, and then be convenient for carry out centralized processing with the waste liquid, then open the sealed lid 21 of the sealed lid of ultrafiltration on ultrafiltration jar 17 top and take out the exosome after the ultrafiltration, and then reached centrifugation and the effect of filtering the integration, and reduced the pollution to exosome.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (10)

1. The utility model provides a special ultrafiltration of medicine carrying exosome extraction and separation, super from integrative device, includes workstation (1), its characterized in that: fixed plate (2) fixedly connected with solid fixed cylinder (3) is passed through to one side on workstation (1) top, gu fixed cylinder (3) inboard fixedly connected with connecting seat (4), top outside fixedly connected with outer loop (5) of connecting seat (4), connecting seat (4) top is located inboard fixedly connected with inner ring (6) of outer loop (5), inner ring (6) top fixedly connected with connecting plate (7), connecting plate (7) bottom fixedly connected with motor (8), the output end fixedly connected with link (9) of motor (8), link (9) other end fixedly connected with centrifuge bowl (10), outside fixedly connected with supernatant export (11) of centrifuge bowl (10) upper end, centrifuge bowl (10) bottom fixedly connected with lower floor liquid export (12), the outside of supernatant export (11) and lower floor liquid export (12) is provided with solenoid valve (13) and solenoid valve two (14) respectively The utility model discloses a, outside fixedly connected with funnel (15) of solid fixed cylinder (3) upper end, funnel (15) are located the below of supernatant outlet (11), the bottom fixedly connected with connecting pipe (16) of funnel (15), connecting pipe (16) other end fixedly connected with ultrafiltration jar (17), ultrafiltration jar (17) fixed connection is in the top of workstation (1).

2. The special ultrafiltration and superseparation integrated device for extracting and separating drug-loaded exosomes according to claim 1, which is characterized in that: be provided with solenoid valve three (18) between ultrafiltration jar (17) and connecting pipe (16), the bottom of ultrafiltration jar (17) is provided with solenoid valve four (20), the outside of ultrafiltration jar (17) is provided with negative pressure device (19), ultrafiltration jar (17) top is provided with sealed lid of ultrafiltration (21), the inside upper end of ultrafiltration jar (17) is provided with the milipore filter.

3. The special ultrafiltration and superseparation integrated device for extracting and separating drug-loaded exosomes according to claim 1, which is characterized in that: the device is characterized in that a first conducting slip ring (22) is arranged on the outer side of the supernatant outlet (11), a first conducting slip ring (24) is arranged on the outer side of the lower layer liquid outlet (12), the first conducting slip ring (22) is located at the top end of a first electromagnetic valve (23), the first conducting slip ring (24) is located on the inner side of a second electromagnetic valve (25), the first conducting slip ring (22) and the inner ring of the first conducting slip ring (24) are electrically connected with a first electromagnetic valve (13) and a second electromagnetic valve (14) respectively, and the first electromagnetic valve (23) and the second electromagnetic valve (14) are both electrically connected with a power supply.

4. The special ultrafiltration and superseparation integrated device for extracting and separating drug-loaded exosomes according to claim 1, which is characterized in that: outside fixedly connected with neck cover (26) of solid fixed cylinder (3) upper end, neck cover (26) are located the outside of funnel (15), neck cover (26) upper end is provided with spacing groove (27), neck cover (26) top has protective cover (28) through spacing groove (27) joint, protective cover (28) top corresponds open-ended (29) has been seted up in centrifuging tank (10).

5. The special ultrafiltration and super-separation integrated device for extracting and separating drug-loaded exosomes according to claim 1, which is characterized in that: workstation (1) one side fixedly connected with support (30) on top, support (30) one side is provided with buffer solution storage jar (31), the bottom fixedly connected with hose (32) of buffer solution storage jar (31), hose (32) other end fixedly connected with liquid feeding gun (33), liquid feeding gun (33) block is in the outside of support (30).

6. The special ultrafiltration and superseparation integrated device for extracting and separating drug-loaded exosomes according to claim 1, which is characterized in that: the top of centrifugation jar (10) is provided with super sealed lid (34), super sealed lid (34) top fixedly connected with feed liquor pipe (35) of keeping away, the inboard on feed liquor pipe (35) top is provided with leather plug (36), feed liquor pipe (35) are located opening (29) under.

7. The special ultrafiltration and superseparation integrated device for extracting and separating drug-loaded exosomes according to claim 1, which is characterized in that: the bottom fixedly connected with of solid fixed cylinder (3) goes out liquid fill (37), go out the inside that liquid fill (37) bottom run through workstation (1) and be located drawer (38), drawer (38) inside is provided with waste liquid box (39).

8. The special ultrafiltration and superseparation integrated device for extracting and separating drug-loaded exosomes according to claim 1, which is characterized in that: the top of ultrafiltration jar (17) runs through workstation (1) and is located the inside of drawer (38), the bottom of ultrafiltration jar (17) and play liquid fill (37) all is located the top of waste liquid box (39).

9. The special ultrafiltration and super-separation integrated device for extracting and separating drug-loaded exosomes according to claim 5, which is characterized in that: the control device is characterized in that a control panel (40) is arranged on the outer side of the support (30), and the control panel (40) is electrically connected with the motor (8), the first electromagnetic valve (13), the second electromagnetic valve (14), the third electromagnetic valve (18), the negative pressure device (19) and the fourth electromagnetic valve (20).

10. The special ultrafiltration and super-separation integrated device for extracting and separating drug-loaded exosomes according to claim 4, which is characterized in that: the inboard of spacing groove (27) is provided with the contact protection switch, contact protection switch and control panel (40) electric connection.

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