CN114717102A - Full-automatic nucleic acid detector - Google Patents

Abstract

The invention provides a full-automatic nucleic acid detector, comprising: a frame; the sample module is provided with a sample rack for placing a sample tube, a screwing cap assembly and a holding assembly; the holding assembly is used for fixing the tube body of the sample tube, and the cap screwing assembly is used for uncapping or capping the sample tube; the strip cup holder module is provided with a supporting plate and a plurality of strip cups, and the strip cups are placed on the supporting plate and used for containing reagents required by nucleic acid extraction and extracting nucleic acid; a pipetting module to load pipettes and/or piercing heads; the puncture head is used for puncturing a sealing film which is arranged on the strip cup and used for sealing the reagent; the reagent module is used for storing the detection reagent at low temperature; a consumable module; a centrifugation module for centrifuging a mixture to be amplified; the amplification detection module is used for amplifying and detecting nucleic acid; and a waste material module. The detector body integrates the functions of reagent storage, sample adding, sample transferring, extraction, amplification, centrifugation, detection and waste material collection, and has the advantages of simple and rapid operation, less manual operation and high detection efficiency.

Description

Full-automatic nucleic acid detector

Technical Field

The invention relates to the technical field of medical equipment, in particular to a full-automatic nucleic acid detector.

Background

In the field of molecular biology today, molecular diagnostic techniques are increasingly used. Nucleic acid extraction, detection and quantification of samples has become a common experimental approach. The steps of cracking, cleaning, eluting, amplifying and the like are included in the process of nucleic acid extraction and amplification detection, a standard PCR laboratory is needed for conventional nucleic acid detection, and the steps of sample pretreatment, nucleic acid extraction, detection system preparation, nucleic acid amplification, detection and the like are completed through multi-step instrument use and manual operation by professional nucleic acid detection personnel, so that the problems of long time consumption, complex operation and labor consumption exist.

Disclosure of Invention

In order to achieve the above object, the present invention is achieved by the following technical solutions.

The invention provides a full-automatic nucleic acid detector, comprising:

a frame for forming a load-bearing structure;

the sample module is provided with a sample rack for placing a sample tube, a screwing cap assembly and a holding assembly; the holding component is used for fixing the tube body of the sample tube, and the cap screwing component is used for uncapping or capping the sample tube;

the strip cup holder module is provided with a supporting plate and a plurality of strip cups, and the strip cups are placed on the supporting plate and used for containing reagents required by nucleic acid extraction and extracting nucleic acid;

a pipetting module to load pipettes and/or piercing heads; the puncture head is used for puncturing a sealing film which is arranged on the strip cup and used for sealing the reagent;

the reagent module is used for storing the detection reagent at low temperature;

the consumable module is used for placing consumables;

a centrifugation module for centrifuging a mixture to be amplified;

the amplification detection module is used for amplifying and detecting nucleic acid;

and the waste material module is used for placing waste materials.

Preferably, the frame is provided with an air filter assembly and an ultraviolet lamp.

Preferably, the screw cap assembly comprises:

the first clamping mechanism is used for clamping the tube cap of the sample tube;

the first rotary driving piece is used for driving the first clamping mechanism to rotate so as to screw the cap;

and the three-dimensional driving mechanism is used for driving the first rotary driving piece to move.

Preferably, the clasping assembly comprises:

the second clamping mechanism is used for clamping the sample tube so that the cap screwing component can screw the cap;

the first X-direction driving mechanism is used for driving the second clamping mechanism to translate along the X direction so as to switch the sample tube between the cap screwing station and the suction station.

Preferably, the supporting plate is arranged in the middle area of the rack and is provided with a plurality of clamping grooves which are arranged in parallel and used for clamping the strip cups respectively; the strip cup is provided with a plurality of containing cavities for containing different reagents;

the strip cup holder module further comprises a heating seat and a magnetic suction component; the heating seat corresponds to the position of the containing cavity for containing the digestive juice; the magnetic attraction component is used for adsorbing magnetic beads in the cavity to be cleaned.

Preferably, the reagent module is disposed proximate to the sample module;

the pipetting module comprises:

a second X-direction driving mechanism fixed to the frame;

at least one first pipetting mechanism arranged on the back of the second X-direction driving mechanism and used for transferring the reagent on the strip cup; the first liquid transferring mechanism comprises a first Z-direction driving mechanism and a first liquid transferring part fixed at the driving end of the first Z-direction driving mechanism; the first pipetting part comprises a plurality of first loading heads for loading pipettes and/or puncture heads;

the first Y-direction driving mechanism is connected to the driving end of the front face of the second X-direction driving mechanism;

and the second pipetting mechanism is arranged on one side of the first Y-direction driving mechanism facing the sample module and is used for transferring the samples of the sample module and/or the detection reagents of the reagent module.

Preferably, the consumable module comprises:

the pipette rack is used for placing a plurality of pipettes;

the reaction tube base is used for clamping a plurality of reaction tubes;

the reaction tube cap base is used for clamping a plurality of reaction tube caps;

and the vibration mechanism is connected to the reaction tube base and is used for vibrating and shaking the mixture in the reaction tube.

Preferably, the pipetting module further comprises a fork mechanism provided with a fork plate; the fork plate includes:

the first bayonets are used for forking the reaction tube cap on the reaction tube cap base and pressing the reaction tube cap into the tube opening of the reaction tube;

and the second bayonets are used for forking the reaction tube behind the cap to be transferred to the centrifugal module and/or the amplification detection module.

Preferably, the sample module, the strip cup holder module and the amplification detection module are sequentially arranged along the Y direction; the consumable module and the centrifugal module are arranged in front of the strip cup holder module; the amplification detection module is arranged behind the pipetting module;

the amplification detection module comprises:

an amplification assembly to amplify nucleic acids;

an optical detection assembly for detecting nucleic acid amplification values suspended above the amplification assembly;

and the second Y-direction driving mechanism is fixed on the rack and used for driving the amplification assembly to move along the Y direction so as to be matched with the fork-mounted mechanism to transfer the reaction tube.

Preferably, the amplification detection module further comprises a pressing cover component for pressing the detection pore plate of the optical detection component against the top wall of the incubation chamber of the amplification component;

the optical detection assembly includes:

an optical module for detecting the nucleic acid amplification value;

the driving end of the third Y-direction driving mechanism is connected with the optical module and used for driving the optical module to move along the Y direction so as to switch different detection holes in the Y direction;

and the driving end of the third X-direction driving mechanism is connected with the third Y-direction driving mechanism and is used for driving the third X-direction driving mechanism to drive the optical module to move along the X direction so as to switch different detection holes in the X direction.

Compared with the prior art, the invention has the beneficial effects that:

the invention provides a full-automatic nucleic acid detector, which integrates the functions of reagent storage, sample adding, sample transferring, extracting, amplifying, centrifuging, detecting and waste material collecting, and has the advantages of simple and rapid operation, less manual operation and high detection efficiency.

The foregoing description is only an overview of the technical solutions of the present invention, and in order to make the technical means of the present invention more clearly understood and to be implemented according to the content of the description, the following detailed description is given with reference to the preferred embodiments of the present invention and the accompanying drawings. The detailed description of the present invention is given in detail by the following examples and the accompanying drawings.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without limiting the invention. In the drawings:

FIG. 1 is a schematic perspective view of a detector body according to the present invention;

FIG. 2 is a perspective view of the twist cap assembly of the present invention;

FIG. 3 is a first perspective view of the clasping assembly of the present invention;

FIG. 4 is a perspective view of the clasping assembly of the present invention, which is schematically illustrated in FIG. two;

FIG. 5 is a first schematic perspective view of a strip cup holder module according to the present invention;

fig. 6 is a schematic perspective view of a strip cup holder module according to the present invention;

FIG. 7 is a perspective view of a pipetting module of the invention;

FIG. 8 is a perspective view of a first pipetting mechanism of the invention;

FIG. 9 is a schematic perspective view of a fork plate of the present invention;

FIG. 10 is a schematic perspective view of a reagent module according to the present invention;

FIG. 11 is a schematic perspective view of a consumable module according to the present invention;

FIG. 12 is a structural cross-sectional view of a centrifuge module of the present invention;

FIG. 13 is an exploded view of the centrifuge module of the present invention;

fig. 14 is a schematic perspective view of an interposer according to the present invention;

FIG. 15 is a schematic perspective view of an amplification detection module according to the present invention;

FIG. 16 is a partial cross-sectional view of an amplification module according to the present invention;

fig. 17 is a partial perspective view of the detector body according to the present invention.

In the figure: 100. a detector body;

10. a frame; 11. an air filtration assembly; 12. a partition plate;

20. a sample module; 21. a sample tube; 22. a sample rack; 23. screwing the cap assembly; 231. a first clamping mechanism; 2311. a first jaw; 232. a first rotary drive member; 233. a three-dimensional drive mechanism; 2331. a fourth X-direction driving mechanism; 2332. a fourth Y-direction drive mechanism; 2333. a second Z-direction driving mechanism; 2334. a first transfer member; 2335. a first slider; 2336. a first guide rail; 2337. a second slider; 2338. a second guide rail; 2339. a third slider; 23310. a third guide rail; 24. a clasping component; 241. a second clamping mechanism; 2411. a first fixing plate; 2412. a motor; 2413. a pinion gear; 2414. a bull gear; 2415. a transmission screw rod; 2416. a second adaptor; 2417. a ball slider; 2418. a fourth slider; 2419. a fourth guide rail; 24110. a first cam; 24111. a second jaw; 242. a first X-direction driving mechanism; 243. a fifth slider; 244. a fifth guide rail; 25. a barcode gun assembly;

30. a strip cup holder module; 31. a support plate; 311. a card slot; 32. a strip cup; 321. sealing the film; 322. a cavity; 33. a heating base; 34. the magnetic component; 341. a second rotary drive; 342. a transmission rod; 343. a second cam; 344. a cam guide; 345. a magnetic attraction seat; 351. a second fixing plate; 3511. a chute; 352. a third fixing plate; 353. a first support member; 354. a second support member; 36. a fifth Y drive mechanism;

40. a pipetting module; 41. a pipette; 42. a second X-direction driving mechanism; 43. a first pipetting mechanism; 431. a first Z-direction driving mechanism; 432. a first loading head; 433. an injector; 434. withdrawing the head plate; 44. a first Y-direction drive mechanism; 45. a second pipetting mechanism; 46. a forking mechanism; 461. a fork plate; 4611. a first bayonet; 4612. a second bayonet; 462. a fourth Z-direction driving mechanism;

50. a reagent module; 51. a reagent tube; 52. a heat-preserving cover; 53. a first air duct; 54. a second air duct;

60. a consumable module; 61. a pipette rack; 62. a reaction tube base; 63. a reaction tube cap base; 64. a vibration mechanism; 651. a reaction tube; 652. a reaction tube cap;

70. a centrifuge module; 71. a third driving member; 72. a turntable; 73. a centrifugal base; 731. an installation part; 74. an iron member; 75. a magnet; 76. an adapter plate; 761. a groove; 7611. a fixing hole; 762. a second card slot; 77. a metal shaft sleeve; 78. a pillar; 79. a housing; 791. a fixed part; 792. a notch;

80. an amplification detection module; 81. an amplification module; 811. heating the susceptor; 812. a top cover; 813. a heat insulating sheet; 814. a cooling member; 815. a heat sink; 816. a fan; 817. a fourth fixing plate; 818. a heating plate; 82. an optical detection assembly; 821. an optical module; 822. a third Y-direction drive mechanism; 8221. a stopper; 823. a third X-direction driving mechanism; 824. detecting the pore plate; 8241. a detection hole; 8242. a stop lever; 83. a second Y-direction drive mechanism; 84. a gland assembly; 841. a rotating electric machine; 842. a screw rod; 843. a fisheye ball head; 844. an annular fixed block; 845. briquetting; 846. a second guide post; 85. a first support frame; 86. a second support frame; 87. a first guide post;

90. a waste module; 91. a waste material box; 92. a feed channel;

101. a power supply module;

102. a touch screen is provided.

Detailed Description

The foregoing and other objects, features, aspects and advantages of the present invention will become more apparent from the following detailed description of the present invention when taken in conjunction with the accompanying drawings, wherein like reference characters designate like parts throughout the several views. In the drawings, the shape and size may be exaggerated for clarity, and the same reference numerals will be used throughout the drawings to designate the same or similar components. In the following description, terms such as center, thickness, height, length, front, back, rear, left, right, top, bottom, upper, lower, and the like are used based on the orientation or positional relationship shown in the drawings. In particular, "height" corresponds to the dimension from top to bottom, "width" corresponds to the dimension from left to right, and "depth" corresponds to the dimension from front to back. These relative terms are for convenience of description and are not generally intended to require a particular orientation. Terms concerning attachments, coupling and the like (e.g., "connected" and "attached") refer to a relationship wherein structures are secured or attached to one another either directly or indirectly through intervening structures, as well as both movable or rigid attachments or relationships, unless expressly described otherwise.

The present invention will be further described with reference to the accompanying drawings and the detailed description, and it should be noted that any combination of the embodiments or technical features described below can be used to form a new embodiment without conflict.

Example 1

The present invention provides a full-automatic nucleic acid detecting instrument, as shown in fig. 1 to 17, including an instrument body 100, the instrument body 100 including:

a frame 10 to form a load-bearing structure;

the sample module 20 is provided with a sample rack 22 for placing a sample tube 21, a cap screwing component 23 and a holding component 24; the clasping assembly 24 is used for fixing the tube body of the sample tube 21, and the screwing cap assembly 23 is used for uncapping or capping the sample tube 21;

the strip cup holder module 30 is provided with a supporting plate 31 and a plurality of strip cups 32, wherein the strip cups 32 are placed on the supporting plate 31 and used for containing reagents required by nucleic acid extraction and extracting nucleic acid;

a pipetting module 40 to load pipettes 41 and/or piercing heads; the puncture head is used for puncturing a sealing film 321 which is arranged on the strip cup 32 and is used for sealing the reagent;

a reagent module 50 for storing a detection reagent at a low temperature;

a consumable module 60 for placing consumables;

a centrifugation module 70 for centrifuging the mixture to be amplified;

an amplification detection module 80 for amplifying and detecting nucleic acid;

a waste module 90 for placing waste.

Specifically, the sample module 20 is provided to realize the functions of sample loading, uncapping, sampling, sample loading and capping, that is, an operator loads the sample tube 21 with the sample on the sample rack 22, uncapping is realized in cooperation with screwing the cap assembly 23 and the clasping assembly 24, sampling is realized in cooperation with the pipetting module 40, and the sample is added to the strip cup 32 for nucleic acid extraction. Arranging a pipetting module 40, loading a piercing head to pierce the sealing membrane 321 on the strip cup 32 to match with subsequent sample adding; pipettes 41 are loaded by the pipetting module 40 to effect pipetting. The reagent module 50 is provided to store the detection reagent at a low temperature. The centrifuge module 70 is provided to perform centrifugation. The detector body 100 integrates the functions of reagent storage, sample adding, sample transferring, extraction, amplification, centrifugation, detection and waste material collection, and has the advantages of simple and rapid operation, less manual operation and high detection efficiency.

In one embodiment, as shown in fig. 1, the housing 10 is provided with an air filter assembly 11 and an ultraviolet lamp, the air filter assembly 11 is used for filtering the air in the detector body 100, and the ultraviolet lamp is used for ultraviolet sterilization to prevent pollution.

In one embodiment, as shown in fig. 2, the screw cap assembly 23 includes:

a first clamping mechanism 231 for clamping the cap of the sample tube 21;

a first rotary driving member 232 for driving the first clamping mechanism 231 to rotate for screwing the cap;

a three-dimensional driving mechanism 233 for driving the first rotary driving member 232 to move. Specifically, the three-dimensional driving mechanism 233 drives the first rotary driving member 232 to drive the first clamping mechanism 231 to move in the X direction, the Y direction, or the Z direction, so as to adjust the position of the first clamping mechanism 231 to match the position of the sample tube 21, thereby implementing the cap screwing.

In one embodiment, the three-dimensional driving mechanism 233 includes a fourth X-direction driving mechanism 2331, a fourth Y-direction driving mechanism 2332, and a second Z-direction driving mechanism 2333. The fourth Y-drive mechanism 2332 is fixed to the rack 10 by a joint block, and the fourth Y-drive mechanism 2332 is located above the side of the sample rack 22 near the edge of the rack 10. The fourth X-direction drive mechanism 2331 is connected to the drive end of a fourth Y-direction drive mechanism 2332. The second Z-direction drive mechanism 2333 is connected to the drive end of the fourth X-direction drive mechanism 2331.

In one embodiment, the fourth X-direction drive mechanism 2331 is a belt drive mechanism.

In one embodiment, the fourth Y-drive mechanism 2332 is a belt drive mechanism.

In one embodiment, the second Z-drive mechanism 2333 is a lead screw and nut mechanism.

In an embodiment, a first connecting member 2334 is disposed below the fourth X-direction driving mechanism 2331, and the first connecting member 2334 is disposed across the fourth Y-direction driving mechanism 2332, which is favorable for reducing the overall size of the three-dimensional driving mechanism 233. The first connecting member 2334 supports and guides the movement of the fourth X-direction driving mechanism 2331 in the Y-direction by the sliding fit of the two first sliding blocks 2335 and the two first guiding rails 2336.

In one embodiment, the fourth X-direction driving mechanism 2331 and the second Z-direction driving mechanism 2333 are respectively provided with a first housing and a second housing to improve the outer contour regularity and the appearance of the three-dimensional driving mechanism 233.

Further, the first housing is open at the front to expose the output end of the fourth X-direction drive mechanism 2331 inside. The fourth X-direction driving mechanism 2331 and the second Z-direction driving mechanism 2333 are respectively provided with a second guide rail 2338 and a second slider 2337 to support and guide the movement of the second Z-direction driving mechanism 2333 in the X-direction. The second block 2337 is U-shaped; two ends of the second rail 2338 are fixed to two ends of the first housing, and a gap is formed between the second rail 2338 and the front surface of the first housing for the second slider 2337 to pass through, so that the second slider 2337 is connected to the output end of the fourth X-direction driving mechanism 2331 located in the first housing.

In one embodiment, the first rotary driving member 232 and the second Z-direction driving mechanism 2333 support and guide the movement of the first rotary driving member 232 in the Z-direction through the third block 2339 and the third rail 23310.

In one embodiment, the first clamping mechanism 231 includes a clamping driving member and two first clamping jaws 2311, and the clamping driving member is configured to drive the two first clamping jaws 2311 to move relatively or oppositely, so as to adjust the distance between the first clamping jaws 2311, thereby performing the clamping or releasing function.

In one embodiment, as shown in fig. 3 and 4, the clasping assembly 24 comprises:

a second clamping mechanism 241 for clamping the sample tube 21 to cap the cap screwing assembly 23;

the first X-direction driving mechanism 242 drives the second clamping mechanism 241 to translate along the X direction, so as to switch the sample tube 21 between the cap screwing station and the suction station. Specifically, the first X-direction driving mechanism 242 is used to adjust the position of the second clamping mechanism 241, and in order to simplify the control design of the first X-direction driving mechanism 242, the second clamping mechanism 241 has two stations, i.e., a cap screwing station and a suction station. When the second clamping mechanism 241 needs to clamp the sample tube 21 for the cap screwing operation, the first X-direction driving mechanism 242 drives the second clamping mechanism 241 to move to the cap screwing station. When the sample tube 21 clamped by the second clamping mechanism 241 needs to be transferred to the suction station for the pipetting module 40 to sample after uncapping, the first X-direction driving mechanism 242 drives the second clamping mechanism 241 to move to the suction station.

In one embodiment, the first X-direction driving mechanism 242 is a belt transmission mechanism.

In one embodiment, the second clamping mechanism 241 is located above the first X-drive mechanism 242 to reduce the lateral dimension of the clasping assembly 24 to take full advantage of the longitudinal space within the housing 10.

In one embodiment, the second clamping mechanism 241 includes:

a first fixing plate 2411 for forming a supporting structure, which is connected to the driving end of the first X-direction driving mechanism 242;

a motor 2412 for forming a rotational driving force;

a pinion 2413 coaxially connected to a drive end of the motor 2412;

a large gear 2414 engaged with the small gear 2413;

one end of the transmission screw rod 2415 is rotatably connected to a second adapter 2416 fixed on the first fixing plate 2411, and the bull gear 2414 is sleeved on the periphery of the transmission screw rod 2415;

a ball sliding block 2417 which is matched with the transmission screw 2415;

a fourth slider 2418 connected below the ball slider 2417;

a fourth guide rail 2419 disposed on the first fixing plate 2411 and slidably connected to the fourth slider 2418;

a first cam 24110 connected above the ball slider 2417;

two second clamping jaws 24111, wherein one ends of the two second clamping jaws 24111 are respectively positioned at two sides of the first cam 24110;

the motor 2412 rotates, the small gear 2413 drives the large gear 2414 to rotate, and under the transmission of the transmission screw 2415 and the ball sliding block 2417, the first cam 24110 moves towards or away from the two second clamping jaws 24111, so that the distance between the two second clamping jaws 24111 is adjusted through the change of the state that the outer contour of the first cam 24110 is abutted against the two second clamping jaws 24111, and the clamping or loosening effect is achieved.

In an embodiment, the first X-direction driving mechanism 242 and the second clamping mechanism 241 are supported and guided by the fifth slider 243 and the fifth guide rail 244.

In one embodiment, as shown in fig. 1 and 17, the sample module 20 includes a barcode gun assembly 25 for scanning barcode information on the sample rack 22.

In an embodiment, as shown in fig. 1 and 17, the supporting plate 31 is disposed in a middle region of the rack 10 to facilitate sampling and sample adding of the strip cups 32, and is provided with a plurality of parallel slots 311 for respectively clamping the strip cups 32; the strip cup 32 is provided with a plurality of cavities 322 for containing different reagents;

as shown in fig. 5 and 6, the strip cup holder module 30 further includes a heating seat 33 and a magnetic attraction component 34; the heating seat 33 corresponds to the cavity 322 for containing the digestive juice; the magnetic attraction component 34 is used for attracting magnetic beads of the cavity 322 to be cleaned. Specifically, the slot 311 is designed to facilitate the assembly and disassembly of the strip cup 32. The number of the cavities 322 of the strip cup 32 is determined according to the type of the reagents to be contained, such as empty cups for respectively containing digestive juice, lysate, protease, cleaning solution 1, cleaning solution 2, cleaning solution 3, eluent, freeze-dried powder and purified nucleic acid for storage and temporary storage.

In one embodiment, as shown in FIG. 17, the reagent module 50 is disposed proximate to the sample module 20;

as shown in fig. 7 and 8, the pipetting module 40 includes:

a second X-direction drive mechanism 42 fixed to the frame 10;

at least one first pipetting mechanism 43 disposed on the back of the second X-direction driving mechanism 42 for transferring the reagent on the strip cup 32; the first pipetting mechanism 43 comprises a first Z-direction driving mechanism 431 and a first pipetting member fixed at the driving end of the first Z-direction driving mechanism 431; the first pipetting means comprise a number of first loading heads 432 for loading pipettes 41 and/or piercing heads;

a first Y-direction driving mechanism 44 connected to a driving end of the front surface of the second X-direction driving mechanism 42;

a second pipetting mechanism 45 disposed on a side of the first Y-direction driving mechanism 44 facing the sample module 20 for transferring the sample of the sample module 20 and/or the detection reagent of the reagent module 50. Specifically, the first pipetting mechanism 43 is located on the back side of the second X-direction driving mechanism 42, and the first Y-direction driving mechanism 44 and the second pipetting mechanism 45 are located on the front side of the second X-direction driving mechanism 42, so as to balance the load-bearing forces on the front side and the back side of the second X-direction driving mechanism 42, thereby preventing the pipetting module 40 from deforming and displacing due to the load-bearing being deviated to one side after long-term operation, and further preventing the pipetting accuracy from being affected. In addition, the first pipetting mechanism 43 is arranged for pipetting the reagent on the strip cup 32, and the second pipetting mechanism 45 is arranged for pipetting the sample on the sample module 20 or the reagent on the reagent module 50, so that the positions of the sample module 20, the strip cup holder module 30 and the reagent module 50 are designed compactly, and the overall size of the detecting instrument body 100 is reduced.

In one embodiment, the second X-drive mechanism 42 is a belt drive mechanism.

In one embodiment, as shown in fig. 8, the first pipetting means comprises:

a plurality of rows of syringes 433 for forming a pipette or a syringe;

a plurality of first loading heads 432, a first loading head 432 is matched with an outlet of a syringe 433.

Further, the number of the first liquid-moving parts is consistent with the number of the clamping grooves 311 on the supporting plate 31, and the arrangement of the first liquid-moving parts is consistent with the arrangement of the clamping grooves 311 on the supporting plate 31, so that the first liquid-moving mechanism 43 punctures or samples and samples the plurality of cups 32 on the supporting plate 31 at the same time, and the liquid-moving efficiency is improved.

Further, the first pipetting mechanism 43 further includes a retreat plate 434 to automatically retreat the pipettes 41 on the first loading head 432.

In one embodiment, the first Y-drive mechanism 44 is a belt drive mechanism.

In one embodiment, the second pipetting mechanism 45 includes a third Z-drive mechanism and a second pipetting member connected to the third Z-drive mechanism.

In one embodiment, as shown in fig. 5 and 6, the strip cup holder module 30 includes a second fixing plate 351 fixed to the rack 10 to form a supporting structure of the strip cup holder module 30; the strip cup holder module 30 is assembled and then mounted on the frame 10, so that the whole machine can be assembled conveniently.

In one embodiment, the strip cup holder module 30 includes a third fixing plate 352, two first supporting members 353, and a fifth Y-direction driving mechanism 36; the second fixing plate 351 is provided with two sliding slots 3511 extending along the Y direction, and the bottom end of a first supporting member 353 is fixed in one sliding slot 3511; the fifth Y-direction driving mechanism 36 is located between the two sliding slots 3511 and fixed to the second fixing plate 351; both sides of the bottom end of the third fixing plate 352 are slidably connected to the two first supporting members 353, respectively, and the middle portion of the bottom surface of the third fixing plate 352 is connected to the driving end of the fifth Y-directional driving mechanism 36; the supporting plate 31, the heating base 33 and the magnetic component 34 of the strip cup holder module 30 are disposed on the third fixing plate 352. Specifically, the fifth Y-direction driving mechanism 36 drives the third fixing plate 352 to move the supporting plate 31, the heating seat 33, and the magnetic attraction assembly 34 along the Y direction to get close to or away from the first pipetting mechanism 43, so as to cooperate with the pipetting or puncturing operation of the first pipetting mechanism 43.

In one embodiment, the fifth Y-drive mechanism 36 is a motor screw drive mechanism.

In one embodiment, the strip cup holder module 30 further includes a second supporting member 354, the top end and the bottom end of which are respectively fixed to the supporting plate 31 and the third fixing plate 352, so as to form a space between the supporting plate 31 and the third fixing plate 352, the space is used for accommodating the heating seat 33 and the magnetic attraction component 34, the longitudinal space in the rack 10 is effectively utilized, and the lateral space occupied by the strip cup holder module 30 is reduced.

In one embodiment, the magnetically attractive assembly 34 includes:

a second rotary driving member 341 for forming a rotary driving force;

a transmission rod 342 coaxially connected to a driving end of the second rotary driver 341;

two second cams 343 connected to both ends of the driving lever 342, respectively;

two cam guide rods 344 eccentrically connected to the two second cams 343, respectively;

a magnetic attraction seat 345 provided with a plurality of magnets;

wherein, the two cam guide rods 344 are respectively connected with two ends of the magnetic suction seat 345; the second rotating member 341 drives the transmission rod 342 to drive the two second cams 343 to rotate, so that the magnetic attraction seat 345 moves under the cooperation of the second cams 343 and the cam guide rods 344, and further the distance between the magnetic attraction seat 345 and the strip cup 32 on the supporting plate 31 is changed, and further the magnetic beads in the corresponding cavities 322 are adsorbed or the magnetic attraction is removed. The magnetic attraction component 34 is simple and small in structure, stable and efficient in operation, and fully utilizes the space below the supporting plate 31.

Further, the second rotary driving member 341 is a belt transmission mechanism, and the driven wheel thereof is sleeved on the periphery of the transmission rod 342.

In one embodiment, the reagent module 50 is provided with an air duct to prevent cross contamination.

Further, as shown in fig. 10, the reagent module 50 includes:

a plurality of reagent tubes 51 for holding a reagent for detection;

a kit for cryopreservation of the reagent tube 51;

a heat-insulating cover 52 for surrounding the reagent cartridge to prevent the heat of the reagent cartridge from being affected by the outside;

a first air duct 53 disposed below the heat insulating cover 52;

and a second air duct 54 disposed at one side of the heat-insulating cover 52.

Further, the first air duct 53 and/or the second air duct 54 are used for discharging condensed water formed on the surface of the heat-insulating cover 52 due to temperature difference, so as to prevent the working table surface around the reagent module 50 from being wet.

Further, the reagent tube 51 includes an empty tube for performing a premixing operation for different reagents for detection. For example, when the nucleic acid extraction of the strip cup holder module 30 is performed at a certain stage, the second pipetting mechanism 45 can perform sampling and premixing operations on different detection reagents in the reagent tube 51, thereby increasing the detection efficiency.

In one embodiment, as shown in fig. 11, the consumable module 60 includes:

a pipette rack 61 for placing a plurality of pipettes 41;

a reaction tube base 62 for clamping a plurality of reaction tubes 651;

a reaction tube cap base 63 for clamping a plurality of reaction tube caps 652;

a vibration mechanism 64 connected to the reaction tube base 62 for vibrating and shaking the mixture in the reaction tube 651. Specifically, the consumable module 60 is provided with a reaction tube base 62 for placing the reaction tube 651, so that the sample after nucleic acid extraction and the detection reagent are mixed therein. The reaction tube cap base 63 is provided to cap the reaction tube 651. A vibration mechanism 64 is provided to oscillate the capped reaction tube 651.

Further, the reaction tube base 62 is a twenty-four tube base, and the plurality of reaction tubes 651 are arranged in a 3 × 4 array.

Further, the arrangement of the plurality of reaction tube caps 652 on the reaction tube base 62 is consistent with the arrangement of the plurality of reaction tubes 651, so that one row or one column of reaction tubes 651 can be capped at the same time, thereby improving the efficiency.

In one embodiment, as shown in fig. 7, 9, 17, the pipetting module 40 further comprises a fork mechanism 46 provided with a fork plate 461; the fork plate 461 includes:

a plurality of first bayonets 4611 for forking up the reaction cap 652 on the reaction cap base 63 and pressing the reaction cap 652 into the opening of the reaction tube 651;

a plurality of second bayonets 4612 for picking up the capped reaction tubes 651 for transferring to the centrifugation module 70 and/or the amplification detection module 80, wherein the reaction tubes are transferred to the centrifugation module 70 for centrifugation and transferred to the amplification detection module 80 for amplification detection. Specifically, the cap of the reaction tubes 651 and the transfer of the reaction tubes 651 can be simultaneously performed by the fork mechanism 46, so that the operation efficiency of the detecting apparatus body 100 can be improved. In addition, through the design of the fork plate 461, the reaction tube cap 652 can be forked, the reaction tube 651 can also be forked, the fork plate 461 has simple structure and diversified functions, and the miniaturized design of the fork mechanism 46 is facilitated.

In one embodiment, the fork mechanism 46 further includes a fourth Z drive mechanism, and the fork plate 461 is connected to a drive end of the fourth Z drive mechanism. Specifically, the second X-direction driving mechanism 42 is used for driving and adjusting the position of the fork plate 461 in the X-direction, the first Y-direction driving mechanism 44 is used for adjusting the position of the fork plate 461 in the Y-direction, and the fourth Z-direction driving mechanism is used for adjusting the position of the fork plate 461 in the Z-direction, so as to implement the fork-up operation.

In one embodiment, as shown in fig. 1 and 17, the sample module 20, the strip cup holder module 30, and the amplification detection module 80 are sequentially arranged along the Y direction; the consumable module 60 and the centrifuge module 70 are arranged in front of the strip cup holder module 30; the amplification detection module 80 is arranged behind the pipetting module 40; the detector body 100 has a compact structure, and the matched modules are arranged close to each other, so as to accelerate the detection speed;

as shown in fig. 15, the amplification detection module 80 includes:

an amplification assembly 81 for amplifying nucleic acids;

an optical detection assembly 82 for detecting nucleic acid amplification values, which is suspended above the amplification assembly 81;

a second Y-direction driving mechanism 83 fixed to the frame 10 for driving the amplification module 81 to move along the Y-direction to cooperate with the fork mechanism 46 to transfer the reaction tube 651. Specifically, the amplification detection module 80 is disposed behind the pipetting module 40 to fully utilize the space behind the pipetting module 40. In addition, because the amplification detection module 80 is located behind the liquid transfer module 40, the movement of the fork mechanism 46 in the X direction is not limited by the amplification detection module 80, so that the gap between the amplification detection module 80 and the strip cup holder module 30 is conveniently reduced, which is beneficial to the miniaturization design of the detector body 100. The amplification module 81 is movable relative to the optical detection module 82 to accommodate the fork mechanism 46 in front of the second X-direction drive mechanism 42 of the pipetting module 40 to place the centrifuged reaction tube 651 in the amplification module 81. In addition, the optical detection module 82 is suspended above the amplification module 81, and the longitudinal space in the rack 10 is fully utilized to reduce the lateral size of the amplification detection module 80.

Further, the amplification detection module 80 further comprises a pressing cover assembly 84 for pressing the detection well plate 824 of the optical detection assembly 82 against the top wall of the incubation chamber of the amplification assembly 81;

the optical detection assembly 82 includes:

an optical module 821 for detecting the nucleic acid amplification value;

a third Y-direction driving mechanism 822, a driving end of which is connected to the optical module 821, for driving the optical module 821 to move along the Y direction, so as to switch different detection holes in the Y direction;

a third X-direction driving mechanism 823, a driving end of which is connected to the third Y-direction driving mechanism 822, for driving the third X-direction driving mechanism 823 to drive the optical module 821 to move along the X direction, so as to switch the detection holes in different X directions. Specifically, after the amplification module 81 is moved to the front of the pipetting module 40 and the reaction tube 651 is loaded, the amplification module 81 is moved to the lower side of the optical detection module 82 for amplification and detection, but since the amplification module 81 is moved relative to the optical detection module 82, a gap is required between the amplification module 81 and the optical detection module 82 in order to facilitate the movement of the amplification module 81 relative to the optical detection module 82. When the amplification module 81 performs amplification, the top wall of the incubation chamber of the amplification module 81 is pressed down by the pressing cover module 84 to cooperate with the optical detection module 82, improving the accuracy of fluorescence detection. The position of the optical module 821 in the transverse plane is adjusted by the third Y-direction driving mechanism 822 and the third X-direction driving mechanism 823 so as to match different inspection holes.

In one embodiment, as shown in fig. 16, the amplification assembly 81 includes:

a heating base 811 provided with a plurality of holders for fixing the reaction tubes 651;

a top cover 812, which covers the heating base 811, for forming a closed incubation chamber structure surrounding the reaction tubes 651 together with the heating base 811, thereby facilitating heat preservation;

a heat shield 813 disposed above the top cover 812 to prevent heat from the top cover 812 from being transferred to the detection aperture plate 824 of the optical detection assembly 82;

a cooling member 814 disposed below the heating base 811 for cooling the heating base 811 to form an amplification temperature environment with high and low temperature circulation in cooperation with the heating base 811;

the top cover 812 is provided with a plurality of first through holes, the heat insulation sheet 813 is provided with a plurality of second through holes, and the first through holes, the second through holes and the detection holes 8241 on the detection hole plate 824 are matched to form an optical detection channel.

Further, the cooling member 814 is Peltier.

Further, the amplification assembly 81 further comprises a heat sink 815 disposed below the cooling member 814 for accelerating cooling of the heating base 811.

Further, the amplification assembly 81 further comprises a fan 816 disposed below the heat sink 815 for accelerating cooling of the heating base 811.

Further, the top cover 812 is provided with a heating plate 818 on the upper surface thereof, so that the top cover 812 is at a higher temperature during the nucleic acid amplification process, and the reaction cap 652 abutting against the lower surface of the top cover 812 is at a higher temperature, so as to prevent the liquid in the reaction tube 651 from evaporating and condensing on the inner surface of the reaction cap 652, thereby affecting the accuracy of the detection result. The heater chip 818 has a plurality of third through holes for matching with the optical inspection.

In one embodiment, as shown in fig. 15, the amplification assembly 81 includes a fourth fixing plate 817 fixed to the frame 10 to form a support structure; the second Y-direction drive mechanism 83 is fixed to the upper surface of the fourth fixing plate 817.

Further, the amplification detection module 80 includes two first support frames 85 and two second support frames 86, wherein the two first support frames 85 are respectively located at two sides of the amplification assembly 81 and fixed to the fourth fixing plate 817; the two second supporting frames 86 are respectively located at two sides of the detecting aperture plate 824 of the optical detecting assembly 82 and are respectively fixed to the two first supporting frames 85. Two second support brackets 86 are used to fix the gland assembly 84.

Further, the amplification detection module 80 includes a plurality of first guide pillars 87, the top ends of the first guide pillars 87 are slidably connected to the bottom end of the detection hole plate 824 and fixed to the first support frame 85, and the springs are sleeved on the outer peripheries of the first guide pillars 87. The first guide post 87 cooperates with a spring to support the detection orifice plate 824. When the gland assembly 84 presses against the upper surface of the detection well plate 824, the detection well plate 824 acts as a gentle pressure against the top wall of the incubation chamber of the amplification assembly 81, under the buffering of the spring, avoiding damage to the incubation chamber. When the gland assembly 84 is withdrawn, the detection orifice plate 824 rises a certain distance under the spring bias to allow the amplification assembly 81 to move in the Y direction.

In one embodiment, the aperture plate 824 has a stop 8242, the third Y-direction driving mechanism 822 has a stop 8221, and the stop 8242 cooperates with the stop 8221 to limit the stop position of the optical module 821 in the Y-direction.

In one embodiment, the gland assembly 84 includes:

a rotary motor 841 to form a rotary driving force;

a screw 842, the top end of which is connected to the driving end of the rotating motor 841;

a fisheye ball 843 connected to the bottom end of the lead screw 842;

a connecting pin which is sleeved in the fisheye ball head 843;

the bottom end of the fisheye ball head 843 is located in the hollow area of the annular fixed block 844, and two ends of the connecting pin are connected to two side walls of the hollow area of the annular fixed block 844;

a pressing block 845 connected to the annular fixing block 844;

when the rotating motor 841 rotates, the screw 842, the fisheye ball 843 and the connecting pin are matched with each other, so that the rotating power of the rotating motor 841 is converted into upgrading linear motion power of the annular fixing block 844, and the pressing block 845 is driven to move up and down.

Further, the gland assembly 84 further includes a plurality of second guide posts 846 fixed to the detection well plate 824; briquetting 845 is slidably coupled to second guide post 846, and through second guide post 846, plays a guiding role in the movement of briquetting 845.

In one embodiment, the second Y-drive mechanism 83 is a belt drive mechanism.

In one embodiment, the third Y-drive 822 is a belt drive.

In one embodiment, the third X-direction drive mechanism 823 is a belt drive mechanism.

In one embodiment, as shown in fig. 12 to 14, the centrifuge module 70 includes:

a third driving member 71 for forming a rotational driving force;

a dial 72 coaxially connected to an output shaft of the third driver 71;

a centrifugal base 73 connected to the turntable 72; the centrifugal base 73 is provided with a plurality of mounting parts 731;

a plurality of reaction tubes 651 for holding a substance to be centrifuged; the reaction tube 651 is fixed to the mounting part 731;

at least one iron piece 74 is arranged at the bottom of the centrifugal base 73, and a magnet 75 is arranged on one side of the third driving piece 71; when the third driving member 71 stops, the ferrous member 74 attracts the magnet 75, so that the centrifugal base 73 stops at the original position.

In this embodiment, the iron piece 74 is arranged on the centrifugal base 73, the magnet 75 is arranged on one side of the third driving piece 71, and the position of the centrifugal base 73 in the stop motion is positioned through the adsorption effect of the magnet 75 and the iron piece 74, so that the positions of the centrifugal base 73 in the two states are consistent, the automatic resetting effect is realized, the operation of the clamping mechanism or the sampling mechanism is convenient to match, and the whole centrifugal module 70 is simple and small in structure.

In one embodiment, the centrifugal base 73 has a plurality of first engaging grooves for forming the mounting portion 731; a reaction tube 651 is clamped in a first clamping groove, and is simple to assemble and easy to detach.

In one embodiment, when the third driving member 71 stops, a ferrous member 74 on the centrifugal base 73 faces the magnet 75 to form a stronger attraction force, thereby improving the magnetic attraction return effect.

In one embodiment, the centrifugal module 70 further comprises an adapter plate 76 coaxially connected to one end of the third driving member 71; the magnet 75 is eccentrically disposed on the adapter plate 76, so that the magnet 75 can be conveniently assembled and the structural change of the third driving member 71 is small.

Further, the adapter plate 76 is annular, and a groove 761 is formed on the outer periphery thereof; the groove 761 is provided with a fixing hole 7611; the magnet 75 is disposed in the groove 761 and fixed to the fixing hole 7611 by a fastener. The adapter plate 76 has a simple structure, and the magnet 75 is convenient to install.

In one embodiment, the centrifugal module 70 further comprises a metal bushing 77, one end of which is coaxially connected to the outer periphery of the output shaft of the third driving member 71, and the other end of which is coaxially connected to the central shaft hole of the rotary plate 72. Specifically, the metal sleeve 77 is provided to play a role of receiving the third driving element 71 and the turntable 72, and the metal sleeve 77 has high structural strength to enhance the assembly structural strength of the third driving element 71 and the turntable 72.

In an embodiment, a protrusion is disposed at an end of the third driving member 71 facing the rotating disc 72, and the protrusion is engaged with the central hole of the annular adaptor plate 76 to achieve a pre-assembling positioning function between the third driving member 71 and the adaptor plate 76. Further, the third driving member 71 and the adapter plate 76 are fixed by a plurality of fasteners.

In one embodiment, the centrifugal module 70 further includes a plurality of support posts 78 distributed in a circumferential array and fixed to the adapter plate 76 to form a mounting structure of the whole machine. Specifically, when the centrifuge module 70 is installed, if the centrifuge module 70 is installed in a nucleic acid detecting apparatus, the bottom ends of the plurality of support posts 78 are fixed on a working table in the nucleic acid detecting apparatus, so as to support and fix the nucleic acid detecting apparatus.

Further, the top ends of the pillars 78 are fixed on the adapter plate 76, and a plurality of the pillars 78 are circumferentially distributed in an array.

In one embodiment, the centrifugal bases 73 are at least two in number and are arranged in a circumferential array about the output shaft of the third drive member 71. Specifically, the number of the centrifugal bases 73 is increased to ensure the total amount of one centrifugation of the centrifugal module 70, and the size and weight of the centrifugal bases 73 are reduced, so that the requirement for the attraction force between the iron 74 and the magnet 75 on the centrifugal bases 73 is reduced.

Further, both ends of the centrifugal base 73 are rotatably connected to the rotating disc 72; the iron piece 74 is arranged at the bottom of the centrifugal base 73. Specifically, during centrifugation, the third driving member 71 drives the turntable 72 to rotate the centrifugal base 73, and the rotation centrifuges the substance in the reaction tube 651. In addition, the two ends of the centrifugal base 73 and the rotation connection points of the rotating disc 72 form a rotation shaft, the iron member 74 is arranged at the bottom of the centrifugal base 73, so that the center of gravity of the centrifugal base 73 moves downwards, and when the rotating disc 72 drives the centrifugal base 73 to rotate around the output shaft of the third driving member 71, the centrifugal base 73 rotates around the rotation shaft formed at the two ends thereof relative to the rotating disc 72, thereby playing a centrifugal role. That is, during centrifugation, the centrifuge base 73 performs two types of rotational movements including a rotational movement around the output shaft of the third driver 71 and a rotational movement around the rotation shaft formed at both ends of the centrifuge base 73, and the centrifugation effect is excellent.

In an embodiment, the third driving member 71 is a hollow cup motor, so as to realize high-speed rotation and instantaneous stop, and to cooperate with magnetic attraction reset to realize precise positioning.

In one embodiment, the centrifugal base 73 and the reaction tube 651 are made of POM material to reduce the weight, so that the third driving member 71 can drive the turntable 72 to rotate and the centrifugal base 73 can drive the reaction tube 651 to rotate relative to the turntable 72.

In one embodiment, the iron member 74 is a screw, which is simple in structure and easy to assemble; a plurality of screws are evenly distributed at the bottom of the centrifugal base 73, so that the iron piece 74 and the magnet 75 are controlled by suction force.

In one embodiment, the centrifuge module 70 further comprises:

an optical coupler connected to the third driver 71;

a stopper provided on the turntable 72;

the optical coupler is matched with the blocking piece to acquire the position information of the rotary disc 72, so that the accurate stop position of the centrifugal base 73 can be accurately controlled.

Further, the adapter plate 76 is provided with a second clamping groove 762 for clamping the optical coupler, and the assembly is simple and quick.

Furthermore, the blocking sheet is integrally formed on the turntable 72, so that the structure is simple and the processing is convenient.

In one embodiment, the turntable 72 and the centrifugal base 73 are hinged by matching shaft sleeves and hinge pins, and the assembly is simple and quick.

In one embodiment, centrifuge module 70 further includes a cover 79 to form a protective cover and enhance the aesthetic appearance of the exterior surface of centrifuge module 70.

Furthermore, fixing portions 791 are disposed on two sides of the outer cover 79, and the fixing portions 791 are provided with mounting holes for mounting on a mounted table. When the centrifuge module 70 is installed in the nucleic acid detecting apparatus, the fixing portion 791 is installed to a table top in the nucleic acid detecting apparatus by a fastener. That is, the outer cover 79 plays a protective role while reducing the assembly structure between the outer cover 79 and the components located therein, which is advantageous to simplify the overall structure of the centrifuge module 70.

In one embodiment, the cover 79 has a notch 792 in its upper surface for observing whether the centrifuge base 73 stops in place.

In one embodiment, the number of the centrifugal bases 73 is two, the notches 792 correspond to the positions of the magnets 75, and the cross-sectional dimension of the notches 792 is larger than that of the centrifugal bases 73, so as to observe whether the centrifugal bases 73 attracted to the magnets 75 stop at the original positions when the third driving member 71 stops, thereby ensuring the accuracy of the reset.

In one embodiment, the rack 10 includes a partition 12 to divide the internal region of the rack 10 into upper and lower regions, and the sample module 20, the strip cup holder module 30, the pipetting module 40, the reagent module 50, the consumable module 60, the centrifugation module 70, and the amplification detection module 80 are located above the partition 12.

In one embodiment, the waste module 90 includes a waste box 91, a feed channel 92, the feed channel 92 being located above the partition 12, and the waste box 91 being located below the partition 12.

Further, the waste module 90 further includes two inductive optocouplers for respectively sensing whether the waste box 91 is filled with waste and whether the waste box 91 is installed in place.

In one embodiment, the monitor body 100 further includes a power module 101 for supplying power.

In one embodiment, the apparatus body 100 further includes a touch screen 102 for an operator to input commands.

The foregoing is merely a preferred embodiment of the invention and is not intended to limit the invention in any manner; those skilled in the art can readily practice the invention as shown and described in the drawings and detailed description herein; however, those skilled in the art should appreciate that they can readily use the disclosed conception and specific embodiments as a basis for designing or modifying other structures for carrying out the same purposes of the present invention without departing from the scope of the invention as defined by the appended claims; meanwhile, any changes, modifications, and evolutions of the equivalent changes of the above embodiments according to the actual techniques of the present invention are still within the protection scope of the technical solution of the present invention.

Claims (10)

1. A fully automatic nucleic acid detecting instrument, comprising:

a frame (10) to form a load-bearing structure;

the sample module (20) is provided with a sample rack (22) for placing a sample tube (21), a cap screwing component (23) and a holding component (24); the clasping assembly (24) is used for fixing the tube body of the sample tube (21), and the screwing cap assembly (23) is used for uncapping or capping the sample tube (21);

the strip cup holder module (30) is provided with a supporting plate (31) and a plurality of strips of cups (32), wherein the strips of cups (32) are placed on the supporting plate (31) and used for containing reagents required by nucleic acid extraction and extracting nucleic acid;

a pipetting module (40) to load pipettes (41) and/or piercing heads; the puncture head is used for puncturing a sealing film (321) which is arranged on the strip cup (32) and is used for sealing the reagent;

a reagent module (50) for cryogenically storing a detection reagent;

a consumable module (60) for placing consumables;

a centrifugation module (70) for centrifuging the mixture to be amplified;

an amplification detection module (80) for nucleic acid amplification and detection;

a waste module (90) for holding waste.

2. The apparatus according to claim 1, wherein the housing (10) is provided with an air filter assembly (11) and an ultraviolet lamp.

3. The fully automated nucleic acid detecting apparatus according to claim 1, wherein the screw cap assembly (23) comprises:

a first clamping mechanism (231) for clamping a cap of the sample tube (21);

the first rotary driving piece (232) is used for driving the first clamping mechanism (231) to rotate so as to screw the cap;

a three-dimensional driving mechanism (233) for driving the first rotary driving member (232) to move.

4. The fully automated nucleic acid detecting apparatus according to claim 3, wherein the clasping assembly (24) comprises:

a second clamping mechanism (241) for clamping the sample tube (21) to cap the cap screwing assembly (23);

a first X-direction driving mechanism (242) for driving the second clamping mechanism (241) to translate along the X direction so as to switch the sample tube (21) between the cap screwing station and the suction station.

5. The automatic nucleic acid detecting instrument according to claim 1, wherein the supporting plate (31) is disposed in a middle region of the rack (10) and has a plurality of parallel slots (311) for respectively receiving a plurality of the strip cups (32); the strip cup (32) is provided with a plurality of containing cavities (322) for containing different reagents;

the strip cup holder module (30) further comprises a heating seat (33) and a magnetic suction component (34); the heating seat (33) corresponds to the position of a cavity (322) for containing digestive juice; the magnetic attraction component (34) is used for adsorbing magnetic beads of the cavity (322) to be cleaned.

6. The apparatus according to claim 1, wherein the reagent module (50) is disposed adjacent to the sample module (20);

the pipetting module (40) comprises:

a second X-direction drive mechanism (42) fixed to the frame (10);

at least one first pipetting mechanism (43) arranged on the back of the second X-direction driving mechanism (42) and used for transferring the reagent on the strip cup (32); the first pipetting mechanism (43) comprises a first Z-direction driving mechanism (431) and a first pipetting part fixed at the driving end of the first Z-direction driving mechanism (431); the first pipetting means comprise a number of first loading heads (432) for loading pipettes (41) and/or piercing heads;

a first Y-direction driving mechanism (44) connected to a driving end of the front face of the second X-direction driving mechanism (42);

a second pipetting mechanism (45) arranged on the side of the first Y-direction driving mechanism (44) facing the sample module (20) for transferring the sample of the sample module (20) and/or the detection reagent of the reagent module (50).

7. The fully automated nucleic acid testing instrument according to any one of claims 1 to 6, wherein the consumable module (60) comprises:

a pipette rack (61) for placing a number of said pipettes (41);

a reaction tube base (62) for clamping a plurality of reaction tubes (651);

the reaction tube cap base (63) is used for clamping a plurality of reaction tube caps (652);

a vibrating mechanism (64) connected to the reaction tube base (62) for vibrating and shaking the mixture in the reaction tube (651).

8. The fully automated nucleic acid detecting apparatus according to claim 7, wherein the pipetting module (40) further comprises a fork mechanism (46) provided with a fork plate (461); the fork plate (461) comprises:

a plurality of first bayonets (4611) for forking the reaction tube cap (652) on the reaction tube cap base (63) and pressing the reaction tube cap (652) into the mouth of the reaction tube (651);

a number of second bayonets (4612) for forking the capped reaction tubes (651) for transfer to the centrifuge module (70) and/or the amplification detection module (80).

9. The fully automatic nucleic acid detecting instrument according to claim 8, wherein the sample module (20), the strip cup holder module (30), and the amplification detecting module (80) are arranged in sequence along a Y direction; the consumable module (60) and the centrifugal module (70) are arranged in front of the strip cup holder module (30); the amplification detection module (80) is arranged behind the pipetting module (40);

the amplification detection module (80) comprises:

an amplification component (81) for amplifying nucleic acids;

an optical detection assembly (82) for detecting nucleic acid amplification values, suspended above the amplification assembly (81);

and the second Y-direction driving mechanism (83) is fixed on the frame (10) and used for driving the amplification component (81) to move along the Y direction so as to cooperate with the fork-mounting mechanism (46) to transfer the reaction tube (651).

10. The apparatus according to claim 9, wherein the amplification and detection module (80) further comprises a pressing and covering assembly (84) for pressing the detection well plate (824) of the optical detection assembly (82) against the top wall of the incubation chamber of the amplification assembly (81);

the optical detection assembly (82) comprises:

an optical module (821) for detecting the nucleic acid amplification value;

a third Y-direction driving mechanism (822), the driving end of which is connected with the optical module (821) and is used for driving the optical module (821) to move along the Y direction so as to switch different detection holes in the Y direction;

and the driving end of the third X-direction driving mechanism (823) is connected with the third Y-direction driving mechanism (822) and used for driving the third X-direction driving mechanism (823) to drive the optical module (821) to move along the X direction so as to switch different detection holes in the X direction.

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