CN114705518B - 悬浮细胞孵育染色方法及免疫荧光检测方法 - Google Patents
悬浮细胞孵育染色方法及免疫荧光检测方法 Download PDFInfo
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- CN114705518B CN114705518B CN202111673716.4A CN202111673716A CN114705518B CN 114705518 B CN114705518 B CN 114705518B CN 202111673716 A CN202111673716 A CN 202111673716A CN 114705518 B CN114705518 B CN 114705518B
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- 210000004027 cell Anatomy 0.000 claims description 84
- 238000004140 cleaning Methods 0.000 claims description 26
- 239000007853 buffer solution Substances 0.000 claims description 16
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- 210000001178 neural stem cell Anatomy 0.000 claims description 7
- 238000007865 diluting Methods 0.000 claims description 6
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- 239000000872 buffer Substances 0.000 claims description 4
- 238000005119 centrifugation Methods 0.000 claims description 4
- 229930040373 Paraformaldehyde Natural products 0.000 claims description 3
- 238000012758 nuclear staining Methods 0.000 claims description 3
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
- G01N2001/2846—Cytocentrifuge method
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
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Priority Applications (1)
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CN202111673716.4A CN114705518B (zh) | 2021-12-31 | 2021-12-31 | 悬浮细胞孵育染色方法及免疫荧光检测方法 |
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CN202111673716.4A CN114705518B (zh) | 2021-12-31 | 2021-12-31 | 悬浮细胞孵育染色方法及免疫荧光检测方法 |
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CN114705518A CN114705518A (zh) | 2022-07-05 |
CN114705518B true CN114705518B (zh) | 2024-07-30 |
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN110376044A (zh) * | 2019-08-29 | 2019-10-25 | 北京银丰鼎诚生物工程技术有限公司 | 一种神经球免疫荧光染色的方法 |
CN111474358A (zh) * | 2020-04-16 | 2020-07-31 | 武汉巴菲尔生物技术服务有限公司 | 一种3d立体免疫荧光染色试剂盒及其应用 |
Family Cites Families (2)
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CN108508196B (zh) * | 2018-04-13 | 2020-10-27 | 何韶衡 | 一种检测人调节性t细胞亚型的试剂盒及检测方法 |
US20210389225A1 (en) * | 2018-12-01 | 2021-12-16 | Mingdao Innovation (Beijing) Medical-Tech Co., Ltd. | A flow cytometric detection method for lymphocyte in immune cells |
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- 2021-12-31 CN CN202111673716.4A patent/CN114705518B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN110376044A (zh) * | 2019-08-29 | 2019-10-25 | 北京银丰鼎诚生物工程技术有限公司 | 一种神经球免疫荧光染色的方法 |
CN111474358A (zh) * | 2020-04-16 | 2020-07-31 | 武汉巴菲尔生物技术服务有限公司 | 一种3d立体免疫荧光染色试剂盒及其应用 |
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