CN114703215A - 使用真核细胞发酵表达血管紧张素转化酶2的方法 - Google Patents

使用真核细胞发酵表达血管紧张素转化酶2的方法 Download PDF

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CN114703215A
CN114703215A CN202111405655.3A CN202111405655A CN114703215A CN 114703215 A CN114703215 A CN 114703215A CN 202111405655 A CN202111405655 A CN 202111405655A CN 114703215 A CN114703215 A CN 114703215A
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邢新会
卢元
王怡
廖锡豪
张翀
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Abstract

使用真核细胞发酵表达血管紧张素转化酶2的方法。本申请涉及一种包含ACE2的工程质粒，包含该工程质粒的真核细胞，以及使用该真核细胞作为宿主细胞发酵表达血管紧张素转化酶2(ACE2)的方法。

Description

使用真核细胞发酵表达血管紧张素转化酶2的方法
技术领域
本申请属于生物技术领域，具体涉及一种使用真核细胞作为宿主细胞表达血管紧张素转化酶2(ACE2)的方法。
背景技术
目前已知2019新型冠状病毒SARS-CoV-2的特异性细胞受体是ACE2(Angiotensin-converting enzyme 2，血管紧张素转化酶2)蛋白质，因此以 ACE2受体蛋白质为基础开发新冠病毒防治蛋白质药物和药物快速筛选平台，可作为新型有效的新冠病毒防治策略。首先，受体蛋白质ACE2可以特异性的结合新冠病毒，类似于“抗体”功能实现病毒的中和以及快速排出体外，以实现新冠病毒治疗的效果。第二，受体蛋白质ACE2在不同人群细胞相对保守不易突变，以此作为蛋白质药物可以应对新冠病毒的突变问题，长效且稳定，从而避免传统疫苗和抗体策略的“失效”问题。第三，可结合目前快速发展的合成生物学等手段，快速实现重组蛋白质ACE2药物的理性设计和大规模生产制造。第四，因生物安全性要求和动物模型发展，大大限制了疫苗、抗体和小分子化学药物的研发进程，可以受体蛋白ACE2为基础发展新型冠状病毒药物筛选平台，脱离生物安全实验室要求的限制，可在普通实验室即可进行药物筛选，从而大大加速药物的筛选和准确度，以加速药物的“设计-建立-测试-学习”研发周期。进一步地，可以ACE2为基础，快速准确探究新型冠状病毒表面S蛋白质、研发药物等与受体ACE2的关联作用机制和规律，以及指导药物合成和临床实验研究。
申请人的工作主要聚焦在新型冠状病毒的细胞受体ACE2蛋白质，结合合成生物学等多学科交叉手段，设计和制造新型冠状病毒高效防治重组蛋白质药物。同时，基于这种新兴范式的受体蛋白质ACE2平台，跳脱生物安全性和动物模型束缚，快速筛选疫苗、抗体和化学小分子药物。从中探究新冠病毒表面蛋白、药物和受体ACE2结合规律，以此来指导新型冠状病毒作用和发展机制基础研究，以及进一步指导新冠病毒防治药物合成和后期临床实验研究。
以上应用都需要能够经济、稳定地生产血管紧张素转化酶2(ACE2)以供研究使用；但是根据申请人的知晓，目前在现有技术中还没有关于使用发酵技术表达血管紧张素转化酶2(ACE2)的方法。
发明内容
本申请的技术方案提供了：
1.一种工程质粒，其包含天然ACE2序列、敲除了跨膜区和胞内区的ACE2 序列、或者进一步敲除了胞外区的ACE2序列作为外源基因。
2.如项1所述的工程质粒，其在如项1所述的ACE2序列的下游还包含标签蛋白的序列，并在其上游包含信号肽序列。
3.如项2所述的工程质粒，其中，所述标签蛋白是HIS或Strep-II，优选 HIS。
4.如项3所述的工程质粒，其中，所述工程质粒是pPICZA，所述工程质粒是pPICZA，其携带了敲除了跨膜区和胞内区的截短ACE2序列、或进一步敲除了胞外区的截短ACE2序列；
SEQ ID NO.1、SEQ ID NO.2；
而表达老虎的两种截短的ACE2的核酸序列分别是
SEQ ID NO.3、SEQ ID NO.4；
其中表达牛的两种截短的ACE2的核酸序列分别是
SEQ ID NO.5、SEQ ID NO.6；
而表达斑马鱼的两种截短的ACE2的核酸序列分别是
SEQ ID NO.7、SEQ ID NO.8；
其中表达狗的两种截短的ACE2的核酸序列分别是
SEQ ID NO.9、SEQ ID NO.10；
而表达猫的两种截短的ACE2的核酸序列分别是
SEQ ID NO.11、SEQ ID NO.12；
其中表达雪貂的两种截短的ACE2的核酸序列分别是
SEQ ID NO.13、SEQ ID NO.14；
而表达恒河猴的两种截短的ACE2的核酸序列分别是
SEQ ID NO.15、SEQ ID NO.16；
其中表达穿山甲的两种截短的ACE2的核酸序列分别是
SEQ ID NO.17、SEQ ID NO.18；
而表达土拨鼠的两种截短的ACE2的核酸序列分别是
SEQ ID NO.19、SEQ ID NO.20；
其中表达果子狸的两种截短的ACE2的核酸序列分别是
SEQ ID NO.21、SEQ ID NO.22；
而表达中华鳖的两种截短的ACE2的核酸序列分别是
SEQ ID NO.23、SEQ ID NO.24；
其中表达褐家鼠的两种截短的ACE2的核酸序列分别是
SEQ ID NO.25、SEQ ID NO.26；
而表达马铁菊头蝠的两种截短的ACE2的核酸序列分别是
SEQ ID NO.27、SEQ ID NO.28；
其中表达蝾螈的两种截短的ACE2的核酸序列分别是
SEQ ID NO.29、SEQ ID NO.30；
其中表达野猪的两种截短的ACE2的核酸序列分别是
SEQ ID NO.31、SEQ ID NO.32；
其中表达蛇的两种截短的ACE2的核酸序列分别是
SEQ ID NO.33、SEQ ID NO.34；
其中表达银鲑鱼的两种截短的ACE2的核酸序列分别是
SEQ ID NO.35、SEQ ID NO.36；
其中表达虹鳟鱼的两种截短的ACE2的核酸序列分别是
SEQ ID NO.37、SEQ ID NO.38；
其中表达三文鱼的两种截短的ACE2的核酸序列分别是
SEQ ID NO.39、SEQ ID NO.40；
其中表达亚东鲑的两种截短的ACE2的核酸序列分别是
SEQ ID NO.41、SEQ ID NO.42；
其中表达水貂的两种截短的ACE2的核酸序列分别是
SEQ ID NO.43、SEQ ID NO.44；
其中表达狐狸的两种截短的ACE2的核酸序列分别是
SEQ ID NO.45、SEQ ID NO.46；
其中表达马的两种截短的ACE2的核酸序列分别是
SEQ ID NO.47、SEQ ID NO.48。
5.一种基因工程真核细胞，其包含如项1-4的任一项所述的工程质粒。
6.如项5所述的基因工程真核细胞，其中该基因工程真核细胞改造自巴斯德毕赤酵母(Pichia pastoris)。
7.如项6所述的基因工程真核细胞，其中，所述巴斯德毕赤酵母的菌株为 X33株。
8.通过真核细胞发酵法使用如项5-7的任一项所述的基因工程真核细胞生产血管紧张素转化酶2(ACE2)的方法，该方法包括：
-对宿主细胞进行培养，在培养物中表达ACE2；
-从培养物中对ACE2提取纯化。
9.如项8所述的生产方法，其中，所述培养和表达的条件为，种子液30 摄氏度下摇床培养20-24h，转入BMMY培养基，30摄氏度下甲醇诱导目标蛋白表达72h；收集发酵液上清。
10.如项8所述的生产方法，其中，所述提取纯化至少使用下述步骤：对发酵液上清进行过滤；使用亲和层析法对ACE2进行提取。
11.一种真核细胞，其染色体上导入有SEQ ID NO.1～SEQ ID NO.48中任一项所示的核酸序列以及SEQ ID NO.97所示的核酸序列；
优选所述真核细胞包含如权利要求1-4的任一项所述的工程质粒以及携带SEQ IDNO.97序列的工程质粒，进一步优选：所述真核细胞包含携带 SEQ ID NO.1或2的pPICKαA工程质粒以及携带SEQ ID NO.97序列的 pPICKαA工程质粒。
12.如项11所述的真核细胞，其中该真核细胞为酵母菌，最优选为巴斯德毕赤酵母(Pichia pastoris)。
13.如项12所述的真核细胞，其中所述巴斯德毕赤酵母的菌株为X33株。
14.通过真核细胞发酵法使用如项11-13的任一项所述的真核细胞共表达血管紧张素转化酶2(ACE2)和新冠棘突蛋白的受体结合域(RBD)的方法，所述方法包括：
-对宿主细胞进行培养，在培养物中共表达ACE2和RBD；
-从培养物中对ACE2和RBD提取纯化。
15.如项14所述的生产方法，其中，所述培养和表达的条件为，种子液 30摄氏度下摇床培养20-24h，转入BMMY培养基，30摄氏度下甲醇诱导目标蛋白表达72h；收集发酵液上清。
16.如项14所述的生产方法，其中，所述提取纯化至少使用下述步骤：对发酵液上清进行过滤；使用亲和层析法对ACE2和RBD进行提取。
发明的技术效果
采用本申请的生产方法，能够经济、稳定地利用发酵技术表达或者共表达血管紧张素转化酶2(ACE2)。
附图说明
图1.pPICKαA质粒的结构示意图；
图2.使用毕赤酵母进行ACE2表达，(A)胞内表达产物各组分的Western Blot分析：分泌的以及细胞内表达的hACE2-740/615.S-hACE2-740/615、分泌的hACE2-740/615,I-hACE2-740/615、细胞内表达的hACE2-740/615；(B) 经纯化的S-hACE2-740/615的SDS-PAGE测定以及去糖基化分析；(C)经纯化的I-hACE2-740/615的SDS-PAGE测定；
图3.使用毕赤酵母进行ACE2表达，(A)表达hACE2-740/615的菌株的培养物上清液的Western Blot分析；(B)表达hACE2-740/615的X33菌株和X33/载体的生长曲线；(C)纯化后的hACE2-740/615蛋白质浓度；
图4.使用毕赤酵母进行ACE2表达，hACE2-740/615与S蛋白的结合特征(ELISA检测)：(A)检测hACE2-740/615与抗-hACE2抗体的结合； (B)使用S1蛋白和抗-S1抗体检测hACE2-740/615与S1蛋白的结合；(C) 使用RBD和抗-RBD抗体检测hACE2-740/615与RBD的结合；(D)使用 S1蛋白和抗-RBD抗体检测hACE2-740/615与S1蛋白的结合；(E)使用 RBD和抗-S1抗体检测hACE2-740/615与RBD的结合。
图5.23种不同物种来源(除人类外)ACE2在毕赤酵母中的分泌表达 Western Blot结果；其中，可以检测到成功分泌表达的ACE2蛋白共有16种： Mf、d、At、Te、Rf、Mj、Ml、Dc、Ss、Rn、Ps、Bt、Df、Dr、Pl、Vv；不能成功分泌表达的ACE2蛋白共有7种：Mm、s、Cs、St、Rt、Sal、Ec。
图6.Western Blot结果，显示了：对不能分泌表达的7种ACE2蛋白进行胞内表达，均能成功表达。
图7A和7B.由此两图可以看出hACE2-615以分子量为81.3kDa的单聚体形式存在，而hACE2-740以分子量为184.5kDa的二聚体形式存在。
图8A和8B.由此两图可以看出，对于所有物种，615版本(图8A)的 ACE2蛋白与RBD的结合相比于740版本(图8B)都有10倍左右的下降。
具体实施方式
需要说明的是，在说明书及权利要求当中使用了某些词汇来指称特定组件。本领域技术人员应可以理解，技术人员可能会用不同名词来称呼同一个组件。本说明书及权利要求并不以名词的差异来作为区分组件的方式，而是以组件在功能上的差异来作为区分的准则。如在通篇说明书及权利要求当中所提及的“包含”或“包括”为一开放式用语，故应解释成“包含但不限定于”。说明书后续描述为实施本发明的较佳实施方式，然所述描述乃以说明书的一般原则为目的，并非用以限定本发明的范围。本发明的保护范围当视所附权利要求所界定者为准。
本申请在第一方面涉及一种工程质粒。
在一个具体实施方式中，提供了一种工程质粒，其包含天然ACE2序列、敲除了跨膜区和胞内区的截短的ACE2序列、或进一步敲除了胞外区的截短 ACE2序列作为外源基因。
在本说明书的上下文中，血管紧张素转化酶(ACE2，EC 3.4.15.1)是一种外肽酶。它在体内的主要功能有以下两个：催化血管紧张素I转化为血管紧张素II；使缓激肽失活。血管紧张素转化酶因这两种功能而成为治疗高血压、心力衰竭、糖尿病合并高血压等疾病的理想靶点。血管紧张素转化酶抑制剂能减少血管紧张素II的生成，并增加缓激肽的活性。此外，ACE也能催化血管紧张素(1-9)转化为血管紧张素(1-7)。经研究，ACE2也是新型冠状病毒(SARS-CoV-2)作用的靶点，本申请的目的就是如何经济快速地生产ACE2，从而利用它基于靶点效应研发抗病毒药物。在本说明书的上下文中，rhACE2-740/615是指重组人ACE2。在此，rhACE2-850是指氨基酸序列长度为850的天然ACE2序列，而rhACE2-740为敲除了跨膜区和胞内区的ACE2序列、而rhACE2-615为进一步敲除了部分胞外区的ACE2 序列。在此需要说明，生物膜所含的蛋白叫膜蛋白，是生物膜功能的主要承担者。根据蛋白分离的难易及在膜中分布的位置，膜蛋白基本可分为三大类：外在膜蛋白或称外周膜蛋白、内在膜蛋白或称整合膜蛋白和脂锚定蛋白。膜蛋白包括糖蛋白，载体蛋白和酶等。本申请所涉及的ACE2蛋白就属于酶类的整合膜蛋白。通常，由于跨膜区的存在，会影响膜蛋白的水溶性，所以对于利用原核生物的生产使用ACE2特定截短形式会有利于改进生产效果。
在本说明书的上下文中，“质粒”是指细菌、酵母菌和放线菌等生物中染色体(或拟核)以外的DNA分子，存在于细胞质中(但酵母除外，酵母的 2μm质粒存在于细胞核中)，具有自主复制能力，使其在子代细胞中也能保持恒定的拷贝数，并表达所携带的遗传信息，是闭合环状的双链DNA分子。质粒不是细菌生长繁殖所必需的物质，可自行丢失或人工处理而消除，如高温、紫外线等。质粒携带的遗传信息能赋予宿主菌某些生物学性状，有利于细菌在特定的环境条件下生存。细菌质粒是DNA重组技术中常用的载体。载体是指把一个有用的外源基因通过基因工程手段，送进受体细胞中去进行增殖和表达的工具。将某种目标基因片段重组到质粒中，构成重组基因或重组体。然后将这种重组体经微生物学的转化技术，转入受体细胞(如大肠杆菌)中，使重组体中的目标基因在受体菌中得以繁殖或表达，从而改变寄主细胞原有的性状或产生新的物质。
需要说明，在本说明书的上下文中，被插入质粒的、标识有18-805aa、 18-710aa、18-615aa的序列分别代表全长氨基酸序列的(即天然的、刨除了第1至第17个氨基酸的天然信号肽)、第18个氨基酸至第710个氨基酸截短形式的(即敲除了跨膜区和胞内区的)、第18个氨基酸至第615个氨基酸截短形式的(即敲除了跨膜区、胞内区和部分胞外区的)ACE2序列。
在又一具体实施方式中，提供了工程质粒，其上述ACE2序列的下游还包含标签蛋白的序列，并在其上游包含信号肽序列。
在本说明书的上下文中，在本说明书的上下文中，His、Flag、HA、Myc、 Strep-II都是常用的蛋白标签。由8个氨基酸(Trp-Ser-His-Pro-Gln-Phe-Glu -Lys)构成了Strap-tag-II,Strep-tag技术开发的原理是利用生物素与抗生物素蛋白链菌素(streptavidin)之间的结合反应，streptavidin也被设计为 Strep-Tactin，这样做可以使对Strap-tag-II的亲和力增加近百倍。目前， Strap-tag-II/Strep-Tactin系统已经成为被应用的最广泛的亲和系统之一。
举例来说，His10是指由十个组氨酸残基组成的融合标签，可插入在目的蛋白的C末端或N末端。当做标签使用时，一是能构成表位利于检测；二是构成独特的结构特征(结合配体)利于纯化。组氨酸残基侧链与固态的镍有强烈的吸引力，可用于固定化金属螯合层析(IMAC)，对重组蛋白进行分离纯化。使用His-tag具有以下技术优势：1.标签的分子量小，只有～0.84KD，而GST和蛋白A分别为～26KD和～30KD，一般不影响目标蛋白的功能；2.His 标签融合蛋白可以在非离子型表面活性剂存在的条件下或变性条件下纯化，前者在纯化疏水性强的蛋白得到应用，后者在纯化包涵体蛋白时特别有用，用高浓度的变性剂溶解后通过金属螯和亲和层析去除杂蛋白，使复性不受其它蛋白的干扰，或进行金属螯和亲和层析复性；3.His标签融合蛋白也被用于蛋白质-蛋白质、蛋白质-DNA相互作用研究；4.His标签免疫原性相对较低，可将纯化的蛋白直接注射动物进行免疫制备抗体；5.可应用于多种表达系统，纯化的条件温和；6.可以和其它的亲和标签一起构建双亲和标签。
Flag标签蛋白为编码8个氨基酸的亲水性多肽(DYKDDDDK)，同时载体中构建的Kozak序列使得带有FLAG的融合蛋白在真核表达系统中表达效率更高。FLAG作为标签蛋白，其融合表达目的蛋白后具有以下优点： 1.FLAG作为融合表达标签，其通常不会与目的蛋白相互作用并且通常不会影响目的蛋白的功能、性质，这样就有利用研究人员对融合蛋白进行下游研究。2.融合FLAG的目的蛋白，可以直接通过FLAG进行亲和层析，此层析为非变性纯化，可以纯化有活性的融合蛋白，并且纯化效率高。3.FLAG作为标签蛋白，其可以被抗FLAG的抗体识别，这样就方便通过WesternBlot、ELISA等方法对含有FLAG的融合蛋白进行检测、鉴定。4.融合在N端的 FLAG，其可以被肠激酶切除(DDDK)，从而得到特异的目的蛋白。因此现FLAG标签已广泛的应用于蛋白表达、纯化、鉴定、功能研究及其蛋白相互作用等相关领域。
C-Myc标签蛋白是一个含11个氨基酸的小标签，其氨基酸序列为Glu- Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu，这11个氨基酸作为抗原表位表达在不同的蛋白质框架中仍可识别其相应抗体。C-Myc tag已成功应用在 Western-blot杂交技术、免疫沉淀和流式细胞计量术中,可用于检测重组蛋白质在靶细胞中的表达。
在本说明书的上下文中，“信号肽”是引导新合成的蛋白质向分泌通路转移的短(长度5-30个氨基酸)肽链。
在一个具体实施方式中，所述标签蛋白是HIS或Strep-II，优选HIS。
在又一具体实施方式中，所述工程质粒是pPICZA，其携带了敲除了跨膜区和胞内区的截短的ACE2序列或进一步敲除胞外区的截短的ACE2序列；
其中表达人的两种截短的ACE2的核酸序列分别是
SEQ ID NO.1、SEQ ID NO.2；
而表达老虎的两种截短的ACE2的核酸序列分别是
SEQ ID NO.3、SEQ ID NO.4；
其中表达牛的两种截短的ACE2的核酸序列分别是
SEQ ID NO.5、SEQ ID NO.6；
而表达斑马鱼的两种截短的ACE2的核酸序列分别是
SEQ ID NO.7、SEQ ID NO.8；
其中表达狗的两种截短的ACE2的核酸序列分别是
SEQ ID NO.9、SEQ ID NO.10；
而表达猫的两种截短的ACE2的核酸序列分别是
SEQ ID NO.11、SEQ ID NO.12；
其中表达雪貂的两种截短的ACE2的核酸序列分别是
SEQ ID NO.13、SEQ ID NO.14；
而表达恒河猴的两种截短的ACE2的核酸序列分别是
SEQ ID NO.15、SEQ ID NO.16；
其中表达穿山甲的两种截短的ACE2的核酸序列分别是
SEQ ID NO.17、SEQ ID NO.18；
而表达土拨鼠的两种截短的ACE2的核酸序列分别是
SEQ ID NO.19、SEQ ID NO.20；
其中表达果子狸的两种截短的ACE2的核酸序列分别是
SEQ ID NO.21、SEQ ID NO.22；
而表达中华鳖的两种截短的ACE2的核酸序列分别是
SEQ ID NO.23、SEQ ID NO.24；
其中表达褐家鼠的两种截短的ACE2的核酸序列分别是
SEQ ID NO.25、SEQ ID NO.26；
而表达马铁菊头蝠的两种截短的ACE2的核酸序列分别是
SEQ ID NO.27、SEQ ID NO.28；
其中表达蝾螈的两种截短的ACE2的核酸序列分别是
SEQ ID NO.29、SEQ ID NO.30；
其中表达野猪的两种截短的ACE2的核酸序列分别是
SEQ ID NO.31、SEQ ID NO.32；
其中表达蛇的两种截短的ACE2的核酸序列分别是
SEQ ID NO.33、SEQ ID NO.34；
其中表达银鲑鱼的两种截短的ACE2的核酸序列分别是
SEQ ID NO.35、SEQ ID NO.36；
其中表达虹鳟鱼的两种截短的ACE2的核酸序列分别是
SEQ ID NO.37、SEQ ID NO.38；
其中表达三文鱼的两种截短的ACE2的核酸序列分别是
SEQ ID NO.39、SEQ ID NO.40；
其中表达亚东鲑的两种截短的ACE2的核酸序列分别是
SEQ ID NO.41、SEQ ID NO.42；
其中表达水貂的两种截短的ACE2的核酸序列分别是
SEQ ID NO.43、SEQ ID NO.44；
其中表达狐狸的两种截短的ACE2的核酸序列分别是
SEQ ID NO.45、SEQ ID NO.46；
其中表达马的两种截短的ACE2的核酸序列分别是
SEQ ID NO.47、SEQ ID NO.48。
本申请在第二方面涉及一种真核细胞。
在一个具体实施方式中，提供了一种基因工程真核细胞，其包含上述工程质粒。
在又一具体实施方式中，提供了一种基因工程真核细胞，其中该基因工程真核细胞改造自巴斯德毕赤酵母(Pichia pastoris)。
在本说明书的上下文中，毕赤酵母即巴斯德毕赤酵母(Pichia pastoris)，是甲醇营养型酵母中的一类能够利用甲醇作为唯一碳源和能源的酵母菌。与其它酵母一样，它在无性生长期主要以单倍体形式存在，当环境营养限制时，常诱导2个生理类型不同的接合型单倍体细胞交配，融合成双倍体。巴斯德毕赤酵母的另一个生物学特点是，甲醇代谢所需的醇氧化酶被分选到过氧化物酶体中，形成区域化。以葡萄糖作碳源时，菌体中只有1个或很少几个小的过氧化物酶体，而以甲醇作碳源时，过氧化物酶体几乎占到整个细胞体积的80％，AOX增至细胞总蛋白的35％-40％。因此，当在AOX基因前利用同源重组方式插入外源蛋白基因时，可获得大量表达。同时，根据甲醇酵母这种可以形成过氧化物酶体的特性，既可利用该系统表达一些毒性蛋白和易被降解的酶类，也可用以研究细胞特异区域化的生物发生及其机制和功能。本申请就是巴斯德毕赤酵母表达外源的ACE2蛋白。
在又一具体实施方式中，所述毕赤酵母的菌株为X33。
本申请在第三方面涉及使用上述基因工程真核细胞生产血管紧张素转化酶2(ACE2)的方法。
在一个具体实施方式中，提供了一种利用基因工程真核细胞生产血管紧张素转化酶2(ACE2)的方法，该方法包括：-对宿主细胞进行培养，在培养物中表达ACE2；-从培养物中对ACE2提取纯化。
在又一具体实施方式中，所述培养和表达的条件为，种子液30摄氏度下摇床培养20-24h，转入BMMY培养基，30摄氏度下甲醇诱导目标蛋白表达72h；收集发酵液上清。
在再一具体实施方式中，所述提取纯化至少使用下述步骤：对发酵液上清进行过滤；使用亲和层析法对ACE2进行提取。
本申请在第四方面涉及一种真核细胞以及使用所述真核细胞共表达血管紧张素转化酶2(ACE2)和新冠棘突蛋白受体结合域(RBD)的方法。
在一个具体实施方式中，提供了一种真核细胞，其染色体上导入有SEQ ID NO.1～SEQ ID NO.48中任一项所示的核酸序列以及SEQ ID NO.97所示的核酸序列；优选所述真核细胞包含如权利要求1-4的任一项所述的工程质粒以及携带SEQ ID NO.97序列的工程质粒，进一步优选：所述真核细胞包含携带SEQ ID NO.1或2的pPICKαA工程质粒以及携带SEQID NO.97序列的pPICKαA工程质粒。
在又一具体实施方式中，所述真核细胞为酵母菌，最优选为巴斯德毕赤酵母(Pichiapastoris)。
在再一具体实施方式中，所述巴斯德毕赤酵母的菌株为X33株。
在一个具体实施方式中，提供了一种通过真核细胞发酵法使用前述真核细胞共表达血管紧张素转化酶2(ACE2)和新冠棘突蛋白受体结合域(RBD) 的方法，所述方法包括：
-对宿主细胞进行培养，在培养物中共表达ACE2和RBD；
-从培养物中对ACE2和RBD提取纯化。
在又一具体实施方式中，所述培养和表达的条件为，种子液30摄氏度下摇床培养20-24h，转入BMMY培养基，30摄氏度下甲醇诱导目标蛋白表达 72h；收集发酵液上清。
在再一具体实施方式中，所述提取纯化至少使用下述步骤：对发酵液上清进行过滤；使用亲和层析法对ACE2和RBD进行提取。
<实施例>
下面将参照附图更详细地描述本发明的具体实施例。虽然附图中显示了本发明的具体实施例，然而应当理解，可以以各种形式实现本发明而不应被这里阐述的实施例所限制。相反，提供这些实施例是为了能够更透彻地理解本发明，并且能够将本发明的范围完整的传达给本领域的技术人员。
实施例1使用毕赤酵母表达人ACE2的设计及其结果分析
第一步，构建质粒和选择菌株
巴斯德毕赤酵母菌株X33用于重组蛋白的表达。带有AOX1启动子的质粒pPICZA被用于细胞内表达hACE2-740/615，而pPICZαA载体被用于在α配合因子信号肽的控制下分泌hACE2-740/615。在C末端带有10个HIS 标签的hACE2(18-740aa)基因进行了密码子优化并且由GENEWIZ公司(中国苏州)合成，然后，在用EcoRI/NotI消化之后结合至pPICZαA载体上，以形成pPICZαA-hACE2-740质粒。
随后，pPICZαA-hACE2-740质粒用引物hACE2-615-F(5’-CATCATC ATCATCATCATCATCATCATCATTGAGCGGCCGCCAGCTTTCTA-3’)和引物hACE2-615-R(5’-TCAATGATGATGATGATGATGATGATGATGATG ATCTGCATAAGGTGACCA-3’)进行PCR扩增，随后用DpnI进行消化，转入大肠杆菌DH10B感受态细胞中以生成pPICZαA-hACE2-615质粒。
使用引物ZA-ACE2-F(5’-AGGAATTCACGTGGCCCAGCATGCAGT CCACTATTGAGGAGCA-3’)和引物ZA-ACE2-R(5’-GTCATGTCTAAGG CTAAAACTAGAAAGCTGGCGGCCGC-3’)对人ACE2-740/615基因进行 PCR扩增。同时，使用引物ZA-F(5’-GTTTTAGCCTTAGACATGAC-3’)和 ZA-R(5’-GCTGGGCCACGTGAATTCCT-3’)对载体pPICZA进行PCR扩增。
随后，hACE2-740/615两片段被分别使用吉布森组装试剂盒(New EnglandBioLabs,USA)组装入pPICZA载体。
第二步，转化细胞和培养细胞
按照以前的方法(Liao et al.2019)进行电穿孔操作以将质粒转入巴斯德毕赤酵母X33。来自同样培养皿的重组克隆被收集并在250rpm，30℃下培养20-24小时(50mL摇瓶中的5mL的BMGY：1％酵母提取物，2％蛋白胨， 1.34％的不含氨基酸的酵母氮源，1％的甘油，100mM的磷酸钾，pH6.0)。
然后，OD600为20的菌株在250rpm，30℃下培养(250mL摇瓶中的 20mL的BMMY：1％酵母提取物，2％蛋白胨，1.34％的不含氨基酸的酵母氮源，1％的甲醇，100mM的磷酸钾，pH6.0)。
用1％甲醇流加式发酵24小时，经72-120小时表达hACE2-740/615。
第三步，蛋白质纯化
-收集胞内表达hACE2-740/615的细胞，洗涤，用10mL的结合缓冲液再悬浮，并通过高压均质化进行破碎。
再低温下高速离心，以含有粗蛋白的上清液装载镍-NTA-组氨酸结合树脂，用含有0.25M咪唑的洗脱缓冲液洗脱蛋白。
最后，收集目的蛋白。
-对于分泌hACE2-740/615的菌株，收集上清液，过滤，并装载镍-NTA- 组氨酸结合树脂。
第四步，结果分析
使用SDS-PAGE、Western blotting和ELISA进行分析。
-HACE2-740/615有胞内表达，也有分泌表达。
使用巴斯德毕赤酵母菌株X33，成功地胞内表达且分泌表达了在C端带有HIS标签的人ACE2(18-740aaor 18-615aa)。
使用镍亲和色谱纯化分泌出的hACE2-740/615。然而，hACE2-740/615 的分子量大于理论分子量(84.98/70.6kDa)。人类ACE2有数个N-糖化位点。
然后，用N-糖苷酶F(PNGase F)处理经纯化的hACE2-740/615。结果显示，hACE2-740/615确实在N端被糖基化。另一方面，不能获得在巴斯德毕赤酵母中胞内表达的经纯化的hACE2-740/615。原因可能是，胞内表达的hACE2-740/615不能形成天然构象并倾向于凝聚。
图2使用毕赤酵母进行ACE2表达，(A)胞内表达产物各组分的Western Blot分析：分泌的以及细胞内表达的hACE2-740/615.S-hACE2-740/615、分泌的hACE2-740/615,I-hACE2-740/615、细胞内表达的hACE2-740/615；(B) 经纯化的S-hACE2-740/615的SDS-PAGE测定以及去糖基化分析；(C)经纯化的I-hACE2-740/615的SDS-PAGE测定。
-hACE2-615在巴斯德毕赤酵母中有高分泌表达
图3使用毕赤酵母X33进行ACE2表达，(A)表达hACE2-740/615的菌株的培养物上清液的Western Blot分析；(B)表达hACE2-740/615的菌株的生长曲线；(C)纯化后的hACE2-740/615蛋白质浓度。
在5天的时长内进行了分泌hACE2-740/615的表达，可以发现， hACE2-740/615在72小时达到最高的积累值。hACE2-615的表达水平要显著高于hACE2-740的。重组的和野生型的菌株生长得几乎相同，这暗示了，hACE2-740/615的表达不会影响巴斯德毕赤酵母的生长。进而，在2L摇瓶发酵72小时之后，从2×400mL培养上清液中纯化出了 hACE2-740/615。收获了12.5mL经纯化的蛋白，hACE2-615的浓度是2.3g/L，这个值比hACE2-740高三倍。这些结果揭示了，hACE2-615在巴斯德毕赤酵母中高表达。
-hACE2-740/615结合S蛋白的功能表征
图4使用毕赤酵母进行ACE2表达，hACE2-740/615与S蛋白的结合特征(ELISA检测)：(A)检测hACE2-740/615与抗-hACE2抗体的结合； (B)使用S1蛋白和抗-S1抗体检测hACE2-740/615与S1蛋白的结合；(C) 使用RBD和抗-RBD抗体检测hACE2-740/615与RBD的结合；(D)使用S1蛋白和抗-RBD抗体检测hACE2-740/615与S1蛋白的结合；(E)使用 RBD和抗-S1抗体检测hACE2-740/615与RBD的结合。
为了验证分泌的以及胞内表达的hACE2-740/615对S蛋白的结合，进行了ELISA实验。首先，检测了hACE2-740/615对商购抗-hACE2抗体的结合活性。结果显示，两者都能够结合至该抗体。然后，商购的S1 蛋白和RBD(S1蛋白结合至ACE2的结构)被用于表征hACE2-740/615的结合。发现，此两者，特别是分泌的hACE2-740/615，显示了对S1蛋白和 RBD的有效结合活性。因此，巴斯德毕赤酵母高分泌型的 hACE2-615可以在处理冠状病毒SARS-CoV-2中起重要作用。
ELISA实验的具体操作为，将200μL的RBD加入到96孔板(美国康宁)中进行过夜的4℃孵育，然后使用PBS进行三次洗涤，常温下加入封闭液(200μL)孵育1h，孵育结束使用200μLPBS洗涤三次。加入封闭液(200 μL)稀释的ACE2并孵育1h。然后倒掉样品并使用PBS洗涤三次，添加封闭液稀释的His Ab-HRP并进行1h的孵育，孵育结束后进行三次的PBS洗涤。加入200μLTMB溶液至显色，加入2M的硫酸溶液终止反应并在450nm 下进行吸光度检测。
实施例2使用毕赤酵母表达老虎ACE2的设计及其结果分析
按照实施例1的步骤进行老虎ACE2毕赤酵母表达实验，与实施例1的区别仅在于，质粒所包含的是老虎ACE2(AtACE2)；其对应的序列为：SEQ ID NO.3、SEQ ID NO.4。结果见图5和图6。
实施例3使用毕赤酵母表达牛ACE2的设计及其结果分析
按照实施例1的步骤进行牛ACE2毕赤酵母表达实验，与实施例1的区别仅在于，质粒所包含的是牛ACE2(BtACE2)；其对应的序列为：SEQ ID NO.5、SEQ ID NO.6。结果见图5和图6。
实施例4使用毕赤酵母表达斑马鱼ACE2的设计及其结果分析
按照实施例1的步骤进行斑马鱼ACE2毕赤酵母表达实验，与实施例1 的区别仅在于，质粒所包含的是斑马鱼ACE2(DrACE2)；其对应的序列为： SEQ ID NO.7、SEQ ID NO.8。结果见图5和图6。
实施例5使用毕赤酵母表达狗ACE2的设计及其结果分析
按照实施例1的步骤进行狗ACE2毕赤酵母表达实验，与实施例1的区别仅在于，质粒所包含的是狗ACE2(dACE2)；其对应的序列为：SEQ ID NO.9、SEQ ID NO.10。结果见图5和图6。
实施例6使用毕赤酵母表达猫ACE2的设计及其结果分析
按照实施例1的步骤进行猫ACE2毕赤酵母表达实验，与实施例1的区别仅在于，质粒所包含的是猫ACE2(DcACE2)；其对应的序列为：SEQ ID NO.11、SEQ ID NO.12。结果见图5和图6。
实施例7使用毕赤酵母表达雪貂ACE2的设计及其结果分析
按照实施例1的步骤进行雪貂ACE2毕赤酵母表达实验，与实施例1的区别仅在于，质粒所包含的是雪貂ACE2(DfACE2)；其对应的序列为：SEQ ID NO.13、SEQ ID NO.14。结果见图5和图6。
实施例8使用毕赤酵母表达恒河猴ACE2的设计及其结果分析
按照实施例1的步骤进行恒河猴ACE2毕赤酵母表达实验，与实施例1 的区别仅在于，质粒所包含的是恒河猴ACE2(MmACE2)；其对应的序列为： SEQ ID NO.15、SEQ IDNO.16。结果见图5和图6。
实施例9使用毕赤酵母表达穿山甲ACE2的设计及其结果分析
按照实施例1的步骤进行穿山甲ACE2毕赤酵母表达实验，与实施例1 的区别仅在于，质粒所包含的是穿山甲ACE2(MjACE2)；其对应的序列为： SEQ ID NO.17、SEQ IDNO.18。结果见图5和图6。
实施例10使用毕赤酵母表达土拨鼠ACE2的设计及其结果分析
按照实施例1的步骤进行土拨鼠ACE2毕赤酵母表达实验，与实施例1 的区别仅在于，质粒所包含的是土拨鼠ACE2(MfACE2)；其对应的序列为： SEQ ID NO.19、SEQ IDNO.20。结果见图5和图6。
实施例11使用毕赤酵母表达果子狸ACE2的设计及其结果分析
按照实施例1的步骤进行果子狸ACE2毕赤酵母表达实验，与实施例1 的区别仅在于，质粒所包含的是果子狸ACE2(PlACE2)；其对应的序列为： SEQ ID NO.21、SEQ IDNO.22。结果见图5和图6。
实施例12使用毕赤酵母表达中华鳖ACE2的设计及其结果分析
按照实施例1的步骤进行中华鳖ACE2毕赤酵母表达实验，与实施例1 的区别仅在于，质粒所包含的是中华鳖ACE2(PsACE2)；其对应的序列为： SEQ ID NO.23、SEQ IDNO.24。结果见图5和图6。
实施例13使用毕赤酵母表达褐家鼠ACE2的设计及其结果分析
按照实施例1的步骤进行褐家鼠ACE2毕赤酵母表达实验，与实施例1 的区别仅在于，质粒所包含的是褐家鼠ACE2(RnACE2)；其对应的序列为： SEQ ID NO.25、SEQ IDNO.26。结果见图5和图6。
实施例14使用毕赤酵母表达马铁菊头蝠ACE2的设计及其结果分析
按照实施例1的步骤进行马铁菊头蝠ACE2毕赤酵母表达实验，与实施例1的区别仅在于，质粒所包含的是马铁菊头蝠ACE2(RfACE2)；其对应的序列为：SEQ ID NO.27、SEQ IDNO.28。结果见图5和图6。
实施例15使用毕赤酵母表达蝾螈ACE2的设计及其结果分析
按照实施例1的步骤进行蝾螈ACE2毕赤酵母表达实验，与实施例1的区别仅在于，质粒所包含的是蝾螈ACE2(sACE2)；其对应的序列为：SEQ ID NO.29、SEQ ID NO.30。结果见图5和图6。
实施例16使用毕赤酵母表达野猪ACE2的设计及其结果分析
按照实施例1的步骤进行野猪ACE2毕赤酵母表达实验，与实施例1的区别仅在于，质粒所包含的是野猪ACE2(SsACE2)；其对应的序列为：SEQ ID NO.31、SEQ ID NO.32。结果见图5和图6。
实施例17使用毕赤酵母表达蛇ACE2的设计及其结果分析
按照实施例1的步骤进行蛇ACE2毕赤酵母表达实验，与实施例1的区别仅在于，质粒所包含的是蛇ACE2(TeACE2)；其对应的序列为：SEQ ID NO.33、SEQ ID NO.34。结果见图5和图6。
实施例18使用毕赤酵母表达银鲑鱼ACE2的设计及其结果分析
按照实施例1的步骤进行银鲑鱼ACE2毕赤酵母表达实验，与实施例1 的区别仅在于，质粒所包含的是银鲑鱼ACE2(CsACE2)；其对应的序列为： SEQ ID NO.35、SEQ IDNO.36。结果见图5和图6。
实施例19使用毕赤酵母表达虹鳟鱼ACE2的设计及其结果分析
按照实施例1的步骤进行虹鳟鱼ACE2毕赤酵母表达实验，与实施例1 的区别仅在于，质粒所包含的是虹鳟鱼ACE2(RtACE2)；其对应的序列为： SEQ ID NO.37、SEQ IDNO.38。结果见图5和图6。
实施例20使用毕赤酵母表达三文鱼ACE2的设计及其结果分析
按照实施例1的步骤进行三文鱼ACE2毕赤酵母表达实验，与实施例1 的区别仅在于，质粒所包含的是三文鱼ACE2(SalACE2)；其对应的序列为： SEQ ID NO.39、SEQ IDNO.40。结果见图5和图6。
实施例21使用毕赤酵母表达亚东鲑ACE2的设计及其结果分析
按照实施例1的步骤进行亚东鲑ACE2毕赤酵母表达实验，与实施例1 的区别仅在于，质粒所包含的是亚东鲑ACE2(StACE2)；其对应的序列为： SEQ ID NO.41、SEQ IDNO.42。结果见图5和图6。
实施例22使用毕赤酵母表达水貂ACE2的设计及其结果分析
按照实施例1的步骤进行水貂ACE2毕赤酵母表达实验，与实施例1的区别仅在于，质粒所包含的是水貂ACE2(MlACE2)；其对应的序列为：SEQ ID NO.43、SEQ ID NO.44。结果见图5和图6。
实施例23使用毕赤酵母表达狐狸ACE2的设计及其结果分析
按照实施例1的步骤进行狐狸ACE2毕赤酵母表达实验，与实施例1的区别仅在于，质粒所包含的是狐狸ACE2(VvACE2)；其对应的序列为：SEQ ID NO.45、SEQ ID NO.46。结果见图5和图6。
实施例24使用毕赤酵母表达马ACE2的设计及其结果分析
按照实施例1的步骤进行马ACE2毕赤酵母表达实验，与实施例1的区别仅在于，质粒所包含的是马ACE2(EcACE2)；其对应的序列为：SEQ ID NO.47、SEQ ID NO.48。结果见图5和图6。
实施例25ACE2和RBD在酵母中的共表达
在自然界中，在新冠病毒感染动物的过程中，SARS-CoV-2S-RBD(RBD- 新冠棘突蛋白的受体结合域)蛋白与动物细胞上的ACE2蛋白发生结合。进行此实验的目的是使ACE2和RBD在酵母中共表达，模拟自然界中人类 ACE2和RBD蛋白处于结合状态时的蛋白质构型。
第一步，构建质粒和选择菌株
从金斯瑞合成RBD-StrepII基因，在用EcoRI/SacII消化之后结合至 pPICKαA载体上，以形成pPICZαA-RBD-StrepII质粒
第二步，转化细胞和培养细胞
分别将pPICZαA-hACE2-740/615质粒电转化到X33菌株中，获得 X33/pPICZαA-hACE2-740/615菌株，然后把pPICZαA-RBD-StrepII质粒电转化到X33/pPICZαA-hACE2-740/615菌株，获得ACE2和RBD共表达菌株。来自同样培养皿的重组克隆被收集并在250rpm，30℃下培养20-24小时 (50mL摇瓶中的5mL的BMGY：1％酵母提取物，2％蛋白胨，1.34％的不含氨基酸的酵母氮源，1％的甘油，100mM的磷酸钾，pH6.0)。
然后，OD600为20的菌株在250rpm，30℃下培养(250mL摇瓶中的 20mL的BMMY：1％酵母提取物，2％蛋白胨，1.34％的不含氨基酸的酵母氮源，1％的甲醇，100mM的磷酸钾，pH6.0)。
用1％甲醇流加式发酵24小时，经72-120小时表达hACE2-740/615。
第三步，蛋白质纯化
-收集上清液，过滤，并装载镍-NTA-组氨酸结合树脂。
第四步，Native-PAGE验证
使用纯化的蛋白样品，与loading buffer预混，然后上样到10％非变性PAGE凝胶，120V跑2-3个小时，考马斯蓝染色，再脱色得到胶图。
RBD-StrepII的核酸和氨基酸序列分别如SEQ ID NO.97和SEQ ID NO.98所示。此实验证明人类ACE2和RBD可以在酵母中成功地共表达。
实施例26ACE2单体和二聚体分析
我们采用多角度光散射(MALS)检测仪对hACE2-615aa和hACE2-740 的分子量进行测定。根据测量的分子量来判定是否为单体或二聚体。从图7A 和图7B的结果可以看出hACE2-615以分子量为81.3kDa的单聚体形式存在，而hACE2-740以分子量为184.5kDa的二聚体形式存在。由于得到表达的 hACE2-740更加接近ACE2蛋白在自然状态下的构象，我们预测hACE2-740 与RBC蛋白的结合能力更强并进行了实施例27。
实施例27分别表达的ACE2和RBD的体外结合实验
使用BLI分析(Biolayer interferometry，生物膜光干涉技术)进行hACE2- 740与RBD蛋白的亲和力(结合/解离)测试。带有Fc标签的SARS-CoV-2 RBD从Sino Biological公司购买。缓冲液为购自Gibbico公司的DPBS。ACE2 和SARS-CoV-2RBD之间的结合亲和力是使用Octet RED96e(FortéBio)的通过BLI检测系统确定的。将带有mFc标签的SARS-CoV-2RBD固定在 ProteinA sensortip上(30℃)。将Sensortip浸入ACE2中以测量结合，然后再浸入仅包含pH 7.4的缓冲液DPBS的孔中以测量解离。进行双扣除，将不含SARS-CoV-2RBD的传感器浸入ACE2中以测量结合，然后浸入仅包含 pH 7.4的缓冲液DPBS的孔中以测定解离。使用Octet数据分析软件v11.1 (FortéBio)将对照数据减去并用1：1的结合模型进行拟合。
材料购买：用于BLI分析的ACE2由本实验室合成；用于BLI分析的 SARS-CoV-2RBD带有Fc标签，从Sino Biological公司购买。
本实验证明，所有物种的ACE2-740版本的蛋白与RBD蛋白的亲和力要明显高于ACE2-615版本；且猪、老虎、土拨鼠、牛的ACE2与RBD蛋白亲和力最强(KD<20nM)。结果如图8A和8B所示。
尽管以上结合附图对本发明的实施方案进行了描述，但本发明并不局限于上述的具体实施方案和应用领域，上述的具体实施方案仅仅是示意性的、指导性的，而不是限制性的。本领域的普通技术人员在本说明书的启示下和在不脱离本发明权利要求所保护的范围的情况下，还可以做出很多种的形式，这些均属于本发明保护之列。
序列表
SEQ ID NO.1:hACE2-740(人)核苷酸序列
CAGTCCACTATTGAGGAGCAGGCGAAGACATTCCTTGACAAGTTCAATCACGAAGCAGAAGATTTATTCTACCAGTCATCACTTGCATCATGGAACTATA ACACAAACATCACAGAAGAGAACGTACAGAATATGAACAACGCAGGAGATAAATGGTCAGCATTTCTTAAAGAACAATCAACACTTGCACAAATGTATCCTC TTCAAGAAATCCAGAATTTAACAGTTAAACTTCAACTTCAAGCACTTCAACAGAATGGTTCATCAGTTCTTTCAGAAGATAAATCAAAGCGGTTGAACACAAT CCTTAACACAATGTCAACAATCTACTCTACCGGGAAAGTCTGCAACCCTGATAACCCTCAAGAATGTCTTCTTCTTGAACCTGGACTTAACGAAATCATGGCAA ACTCACTTGATTATAACGAAAGACTTTGGGCATGGGAATCATGGAGATCAGAAGTTGGAAAGCAGCTCAGACCACTCTACGAGGAGTACGTTGTTCTTAAGAA TGAGATGGCAAGAGCAAACCATTATGAAGATTATGGAGATTATTGGAGAGGAGATTATGAAGTTAACGGAGTTGATGGATATGATTATTCAAGAGGTCAGCT AATTGAGGACGTTGAACATACATTTGAAGAAATCAAACCGTTGTACGAGCACCTGCATGCATATGTTAGAGCAAAGCTCATGAACGCATATCCTTCATATATC TCACCTATCGGATGTCTTCCTGCACATCTTCTTGGAGATATGTGGGGCCGTTTCTGGACTAACCTTTATTCACTTACAGTTCCTTTCGGGCAGAAACCAAATATCGATGTTACAGATGCAATGGTTGATCAAGCATGGGATGCACAAAGAATCTTTAAAGAAGCAGAGAAGTTCTTCGTATCTGTTGGACTTCCTAACATGACACAAG GATTCTGGGAGAACTCCATGCTTACAGATCCTGGAAATGTCCAGAAGGCAGTTTGTCATCCTACAGCATGGGATCTTGGAAAGGGTGATTTCCGTATTCTTATG TGTACAAAGGTCACTATGGATGATTTCCTCACTGCACATCATGAAATGGGACATATCCAATATGATATGGCATATGCAGCACAACCTTTCTTATTAAGAAACG GAGCAAACGAAGGATTTCATGAAGCAGTTGGAGAAATCATGTCACTTTCAGCAGCAACACCTAAACATCTTAAATCAATCGGACTTCTTTCACCTGATTTCCA GGAGGATAACGAAACAGAAATCAACTTTCTTCTTAAACAAGCACTTACAATCGTTGGAACACTTCCTTTCACTTACATGCTTGAGAAGTGGCGCTGGATGGTG TTCAAGGGTGAAATCCCTAAAGATCAATGGATGAAGAAGTGGTGGGAAATGAAGAGGGAGATCGTTGGAGTTGTTGAACCTGTTCCTCATGATGAAACATAT TGTGACCCAGCCTCTCTGTTCCACGTGTCTAATGACTACAGTTTCATACGCTACTACACGCGCACCCTATATCAATTTCAATTTCAAGAAGCACTTTGTCAAGC AGCAAAGCACGAGGGACCTCTTCATAAATGTGATATCTCAAACTCAACAGAAGCAGGACAGAAGCTGTTTAATATGCTTAGACTTGGAAAGAGCGAGCCTTG GACACTTGCACTTGAGAATGTAGTTGGAGCAAAGAATATGAACGTTAGACCTCTTCTTAACTATTTCGAGCCATTGTTCACTTGGCTTAAAGATCAGAATAAG AACTCCTTTGTTGGATGGTCAACAGATTGGTCACCTTATGCAGATCAATCAATCAAAGTTAGAATCTCACTTAAATCAGCACTTGGAGATAAAGCATATGAAT GGAACGATAACGAAATGTACCTATTTCGAAGTTCCGTCGCTTACGCTATGCGTCAGTACTTTCTGAAAGTGAAGAATCAAATGATCCTGTTCGGCGAGGAAGA TGTTAGAGTTGCAAACCTTAAACCTAGAATCTCATTTAACTTCTTCGTCACCGCACCTAAGAATGTCTCAGATATCATCCCTAGAACAGAAGTTGAGAAGGCTA TTAGAATGTCAAGATCAAGAATCAACGATGCATTTAGACTTAACGATAACTCACTTGAATTTCTTGGAATCCAACCTACACTTGGACCTCCTAACCAACCTCCT GTTTCA
SEQ ID NO.2:hACE2-615(人)核苷酸序列
CAGTCCACTATTGAGGAGCAGGCGAAGACATTCCTTGACAAGTTCAATCACGAAGCAGAAGATTTATTCTACCAGTCATCACTTGCATCATGGAACTATA ACACAAACATCACAGAAGAGAACGTACAGAATATGAACAACGCAGGAGATAAATGGTCAGCATTTCTTAAAGAACAATCAACACTTGCACAAATGTATCCTC TTCAAGAAATCCAGAATTTAACAGTTAAACTTCAACTTCAAGCACTTCAACAGAATGGTTCATCAGTTCTTTCAGAAGATAAATCAAAGCGGTTGAACACAAT CCTTAACACAATGTCAACAATCTACTCTACCGGGAAAGTCTGCAACCCTGATAACCCTCAAGAATGTCTTCTTCTTGAACCTGGACTTAACGAAATCATGGCAA ACTCACTTGATTATAACGAAAGACTTTGGGCATGGGAATCATGGAGATCAGAAGTTGGAAAGCAGCTCAGACCACTCTACGAGGAGTACGTTGTTCTTAAGAA TGAGATGGCAAGAGCAAACCATTATGAAGATTATGGAGATTATTGGAGAGGAGATTATGAAGTTAACGGAGTTGATGGATATGATTATTCAAGAGGTCAGCT AATTGAGGACGTTGAACATACATTTGAAGAAATCAAACCGTTGTACGAGCACCTGCATGCATATGTTAGAGCAAAGCTCATGAACGCATATCCTTCATATATC TCACCTATCGGATGTCTTCCTGCACATCTTCTTGGAGATATGTGGGGCCGTTTCTGGACTAACCTTTATTCACTTACAGTTCCTTTCGGGCAGAAACCAAATATCGATGTTACAGATGCAATGGTTGATCAAGCATGGGATGCACAAAGAATCTTTAAAGAAGCAGAGAAGTTCTTCGTATCTGTTGGACTTCCTAACATGACACAAG GATTCTGGGAGAACTCCATGCTTACAGATCCTGGAAATGTCCAGAAGGCAGTTTGTCATCCTACAGCATGGGATCTTGGAAAGGGTGATTTCCGTATTCTTATG TGTACAAAGGTCACTATGGATGATTTCCTCACTGCACATCATGAAATGGGACATATCCAATATGATATGGCATATGCAGCACAACCTTTCTTATTAAGAAACG GAGCAAACGAAGGATTTCATGAAGCAGTTGGAGAAATCATGTCACTTTCAGCAGCAACACCTAAACATCTTAAATCAATCGGACTTCTTTCACCTGATTTCCA GGAGGATAACGAAACAGAAATCAACTTTCTTCTTAAACAAGCACTTACAATCGTTGGAACACTTCCTTTCACTTACATGCTTGAGAAGTGGCGCTGGATGGTG TTCAAGGGTGAAATCCCTAAAGATCAATGGATGAAGAAGTGGTGGGAAATGAAGAGGGAGATCGTTGGAGTTGTTGAACCTGTTCCTCATGATGAAACATAT TGTGACCCAGCCTCTCTGTTCCACGTGTCTAATGACTACAGTTTCATACGCTACTACACGCGCACCCTATATCAATTTCAATTTCAAGAAGCACTTTGTCAAGC AGCAAAGCACGAGGGACCTCTTCATAAATGTGATATCTCAAACTCAACAGAAGCAGGACAGAAGCTGTTTAATATGCTTAGACTTGGAAAGAGCGAGCCTTG GACACTTGCACTTGAGAATGTAGTTGGAGCAAAGAATATGAACGTTAGACCTCTTCTTAACTATTTCGAGCCATTGTTCACTTGGCTTAAAGATCAGAATAAG AACTCCTTTGTTGGATGGTCAACAGATTGGTCACCTTATGCAGAT
SEQ ID NO.3:AtACE2-740(老虎)核苷酸序列
TCCACTACTGAGGAATTGGCTAAGACTTTTTTGGAGAAGTTTAACCACGAGGCCGAGGAGTTGTCTTACCAATCTTCTTTGGCTTCTTGGAACTACAACAC TAACATTACCGATGAGAACGTCCAGAAGATGAACGAAGCTGGTGCTAAGTGGTCTGCTTTTTACGAAGAACAATCTAAGTTGGCCGAAACCTACCCATTGGCT GAAATTCATAACACCACTGTTAAGCGTCAGTTGCAGGCTTTGCAACAATCTGGTTCTTCTGTTTTGTCTGCCGATAAGTCTCAAAGATTGAACACTATCTTGAA CGCCATGTCCACTATCTACTCTACTGGTAAGGCCTGTAACCCAAACAACCCACAAGAATGTTTGTTGTTGGAACCAGGTTTGGATGATATTATGGAGAACTCCA AGGACTACAACGAACGTTTGTGGGCTTGGGAAGGTTGGAGAGCTGAAGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTCGCTTTGAAGAACGAGA TGGCCAGAGCTAACAACTACGAAGATTACGGTGATTACTGGAGAGGTGATTACGAAGAAGAATGGACTGATGGTTACAACTACTCTCGTTCTCAATTGATCAA GGACGTCGAACATACCTTCACCCAGATCAAGCCATTGTACCAACACTTGCATGCTTACGTTAGAGCTAAGTTGATGGATTCTTACCCCTCTAGAATTTCCCCAA CTGGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGACTGTCCCATTTGGTCAAAAGCCAAACATTGACGTT ACTGACGCTATGGTTAACCAGTCCTGGGATGCTAGAAGAATTTTCAAGGAGGCTGAAAAGTTTTTCGTCTCCGTTGGTTTGCCAAACATGACTCAAGGTTTTTG GGAAAACTCTATGTTGACCGAACCAGGTAACTCTCAAAAGGTTGTTTGTCATCCAACTGCCTGGGATTTGGGTAAGGGTGATTTTAGAATTAAGATGTGCACC AAGGTCACCATGGACGACTTTTTGACCGCCCACCATGAAATGGGTCATATTCAATACGATATGGCCTACGCCGTTCAGCCATTTTTGTTGAGAAACGGTGCTAA CGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCCGCTGCTACTCCAAACCATTTGAAGACTATTGGTTTGTTGCCACCAGGTTTTTCTGAAGATTC TGAAACTGAAATCAACTTCTTGTTGAAGCAGGCCTTGACTATCGTCGGTACCTTGCCATTTACCTACATGTTGGAGAAGTGGAGATGGATGGTTTTTAAGGGTG AAATTCCAAAGGAGCAGTGGATGCAAAAGTGGTGGGAAATGAAGAGAGAAATTGTCGGTGTTGTCGAGCCAGTTCCACATGATGAAACTTACTGTGATCCAG CTTCTTTGTTTCACGTCGCTAACGATTACTCTTTCATCAGATACTACACCCGCACCATTTACCAGTTCCAGTTTCAGGAAGCTTTGTGCAGAATTGCCAAGCACG AAGGTCCATTGCATAAGTGTGATATTTCTAACTCCTCCGAGGCCGGTAAGAAGTTGTTGCAAATGTTGACTTTGGGCAAGTCCAAGCCATGGACTTTGGCTTTG GAACATGTTGTTGGTGAAAAGAACATGAACGTCACCCCATTGTTGAAGTACTTCGAACCATTGTTTACCTGGTTGAAGGAGCAAAACAGAAACTCTTTCGTCG GTTGGAACACTGATTGGAGACCATACGCTGATCAATCCATCAAGGTCAGAATTTCCTTGAAGTCTGCCTTGGGTGATAAGGCTTACGAATGGAACGATAACGA AATGTACTTGTTCCGTTCCTCTGTTGCTTACGCCATGAGAGAATACTTTTCTAAGGTTAAGAACCAGACCATCCCATTCGTTGAGGATAACGTCTGGGTCTCTA ACTTGAAGCCAAGAATTTCTTTTAACTTCTTCGTCACCGCCTCCAAGAACGTTTCTGATGTTATTCCACGTCGTGAGGTCGAAGAAGCCATTAGAATGTCTCGT TCTAGAATTAACGACGCCTTCCGTTTGGATGACAACTCCTTGGAATTTTTGGGTATTCAGCCAACTTTGTCCCCACCATACCAACCACCAGTTACT
SEQ ID NO.4:AtACE2-615(老虎)核苷酸序列
TCCACTACTGAGGAATTGGCTAAGACTTTTTTGGAGAAGTTTAACCACGAGGCCGAGGAGTTGTCTTACCAATCTTCTTTGGCTTCTTGGAACTACA ACACTAACATTACCGATGAGAACGTCCAGAAGATGAACGAAGCTGGTGCTAAGTGGTCTGCTTTTTACGAAGAACAATCTAAGTTGGCCGAAACCTACCCATT GGCTGAAATTCATAACACCACTGTTAAGCGTCAGTTGCAGGCTTTGCAACAATCTGGTTCTTCTGTTTTGTCTGCCGATAAGTCTCAAAGATTGAACACTATCT TGAACGCCATGTCCACTATCTACTCTACTGGTAAGGCCTGTAACCCAAACAACCCACAAGAATGTTTGTTGTTGGAACCAGGTTTGGATGATATTATGGAGAA CTCCAAGGACTACAACGAACGTTTGTGGGCTTGGGAAGGTTGGAGAGCTGAAGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTCGCTTTGAAGAA CGAGATGGCCAGAGCTAACAACTACGAAGATTACGGTGATTACTGGAGAGGTGATTACGAAGAAGAATGGACTGATGGTTACAACTACTCTCGTTCTCAATTG ATCAAGGACGTCGAACATACCTTCACCCAGATCAAGCCATTGTACCAACACTTGCATGCTTACGTTAGAGCTAAGTTGATGGATTCTTACCCCTCTAGAATTTC CCCAACTGGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGACTGTCCCATTTGGTCAAAAGCCAAACATTGACGTTACTGACGCTATGGTTAACCAGTCCTGGGATGCTAGAAGAATTTTCAAGGAGGCTGAAAAGTTTTTCGTCTCCGTTGGTTTGCCAAACATGACTCAAGGT TTTTGGGAAAACTCTATGTTGACCGAACCAGGTAACTCTCAAAAGGTTGTTTGTCATCCAACTGCCTGGGATTTGGGTAAGGGTGATTTTAGAATTAAGATGTG CACCAAGGTCACCATGGACGACTTTTTGACCGCCCACCATGAAATGGGTCATATTCAATACGATATGGCCTACGCCGTTCAGCCATTTTTGTTGAGAAACGGTG CTAACGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCCGCTGCTACTCCAAACCATTTGAAGACTATTGGTTTGTTGCCACCAGGTTTTTCTGAAG ATTCTGAAACTGAAATCAACTTCTTGTTGAAGCAGGCCTTGACTATCGTCGGTACCTTGCCATTTACCTACATGTTGGAGAAGTGGAGATGGATGGTTTTTAAG GGTGAAATTCCAAAGGAGCAGTGGATGCAAAAGTGGTGGGAAATGAAGAGAGAAATTGTCGGTGTTGTCGAGCCAGTTCCACATGATGAAACTTACTGTGAT CCAGCTTCTTTGTTTCACGTCGCTAACGATTACTCTTTCATCAGATACTACACCCGCACCATTTACCAGTTCCAGTTTCAGGAAGCTTTGTGCAGAATTGCCAAG CACGAAGGTCCATTGCATAAGTGTGATATTTCTAACTCCTCCGAGGCCGGTAAGAAGTTGTTGCAAATGTTGACTTTGGGCAAGTCCAAGCCATGGACTTTGG CTTTGGAACATGTTGTTGGTGAAAAGAACATGAACGTCACCCCATTGTTGAAGTACTTCGAACCATTGTTTACCTGGTTGAAGGAGCAAAACAGAAACTCTTT CGTCGGTTGGAACACTGATTGGAGACCATACGCTGAT
SEQ ID NO.5:BtACE2-740(牛)核苷酸序列
TCCACTACTGAAGAACAAGCTAAGACTTTCTTGGAGAAGTTTAACCACGAGGCCGAAGATTTGTCTTACCAATCTTCTTTGGCTTCCTGGAACTACAACAC TAACATTACCGACGAGAACGTCCAAAAGATGAACGAAGCCAGAGCTAAGTGGTCTGCTTTTTACGAAGAACAATCTCGTATGGCCAAGACTTACTCCTTGGAA GAGATTCAGAACTTGACTTTGAAGCGTCAATTGAAGGCTTTGCAGCACTCTGGTACTTCTGCTTTGTCTGCTGAAAAGTCTAAGAGATTGAACACCATTTTGAA CAAGATGTCCACCATCTACTCCACCGGTAAGGTTTTGGACCCAAACACTCAAGAATGTTTGGCTTTGGAACCAGGTTTGGATGATATTATGGAAAACTCCCGT GACTACAACCGTCGTTTGTGGGCTTGGGAAGGTTGGAGAGCTGAAGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTCGTTTTGGAAAACGAGATG GCCAGAGCTAACAACTACGAAGATTACGGTGATTACTGGAGAGGTGATTACGAAGTTACTGGTGCTGGTGATTACGATTACTCTAGAGATCAATTGATGAAGG ACGTCGAAAGAACTTTCGCCGAAATTAAGCCATTGTACGAGCAATTGCATGCCTACGTTAGAGCTAAGTTGATGCATACTTACCCATCTTACATTTCCCCCACC GGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACTCTTTGACCGTCCCATTTGAGCATAAGCCATCTATTGATGTCACT GAGAAGATGGAAAACCAGTCTTGGGATGCTGAAAGAATTTTTAAGGAGGCCGAAAAGTTCTTCGTCTCCATTTCCTTGCCATACATGACCCAAGGTTTTTGGG ATAACTCTATGTTGACTGAGCCAGGTGATGGTAGAAAGGTTGTTTGTCATCCAACTGCTTGGGATTTGGGTAAGGGTGATTTTAGAATTAAGATGTGCACCAA GGTCACCATGGACGACTTCTTGACTGCTCATCATGAAATGGGTCATATCCAATACGATATGGCCTACGCTGCTCAACCATACTTGTTGAGAAACGGTGCTAAC GAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCTTTGTCCGCTGCTACTCCACATTACTTGAAGGCTTTGGGTTTGTTGGCTCCAGATTTTCATGAAGATAAC GAGACCGAAATTAACTTCTTGTTGAAGCAGGCCTTGACCATCGTTGGTACTTTGCCATTTACTTACATGTTGGAGAAGTGGAGATGGATGGTTTTTAAGGGTGA AATTCCAAAGCAACAGTGGATGGAAAAGTGGTGGGAAATGAAGAGAGAAATTGTCGGTGTTGTCGAGCCATTGCCACATGATGAAACTTACTGTGATCCAGC TTGTTTGTTTCACGTTGCTGAAGATTACTCCTTTATCAGATACTACACCCGTACCATCTACCAGTTCCAATTTCATGAGGCCTTGTGCAAGACTGCTAAGCATGA AGGTGCTTTGTTTAAGTGTGATATCTCCAACTCCACTGAGGCCGGTCAAAGATTGTTGCAAATGTTGAGATTGGGTAAGTCCGAACCATGGACTTTGGCTTTGG AAAACATTGTTGGTATTAAGACCATGGACGTCAAGCCATTGTTGAACTACTTTGAGCCATTGTTTACTTGGTTGAAGGAGCAGAACCGTAACTCTTTTGTCGGT TGGTCTACTGAATGGACTCCATACTCTGATCAATCCATCAAGGTCAGAATCTCTTTGAAGTCCGCTTTGGGTGAGAACGCTTACGAATGGAACGATAACGAAA TGTACTTGTTCCAGTCCTCCGTTGCTTACGCTATGAGAAAGTACTTTTCCGAAGCTCGTAACGAAACTGTTTTGTTCGGTGAAGATAACGTCTGGGTTTCTGATA AGAAGCCAAGAATTTCTTTCAAGTTCTTCGTCACCTCTCCCAACAACGTTTCTGATATCATTCCACGTACCGAGGTTGAAAACGCTATTAGATTGTCTCGTGAT CGTATCAACGATGTCTTTCAATTGGATGACAACTCCTTGGAGTTTTTGGGTATTCAACCAACTTTGGGTCCACCATACGAACCACCAGTTACT
SEQ ID NO.6:BtACE2-615(牛)核苷酸序列
TCCACTACTGAAGAACAAGCTAAGACTTTCTTGGAGAAGTTTAACCACGAGGCCGAAGATTTGTCTTACCAATCTTCTTTGGCTTCCTGGAACTACA ACACTAACATTACCGACGAGAACGTCCAAAAGATGAACGAAGCCAGAGCTAAGTGGTCTGCTTTTTACGAAGAACAATCTCGTATGGCCAAGACTTACTCCTT GGAAGAGATTCAGAACTTGACTTTGAAGCGTCAATTGAAGGCTTTGCAGCACTCTGGTACTTCTGCTTTGTCTGCTGAAAAGTCTAAGAGATTGAACACCATTT TGAACAAGATGTCCACCATCTACTCCACCGGTAAGGTTTTGGACCCAAACACTCAAGAATGTTTGGCTTTGGAACCAGGTTTGGATGATATTATGGAAAACTC CCGTGACTACAACCGTCGTTTGTGGGCTTGGGAAGGTTGGAGAGCTGAAGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTCGTTTTGGAAAACGAG ATGGCCAGAGCTAACAACTACGAAGATTACGGTGATTACTGGAGAGGTGATTACGAAGTTACTGGTGCTGGTGATTACGATTACTCTAGAGATCAATTGATGA AGGACGTCGAAAGAACTTTCGCCGAAATTAAGCCATTGTACGAGCAATTGCATGCCTACGTTAGAGCTAAGTTGATGCATACTTACCCATCTTACATTTCCCCC ACCGGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACTCTTTGACCGTCCCATTTGAGCATAAGCCATCTATTGATGTCACTGAGAAGATGGAAAACCAGTCTTGGGATGCTGAAAGAATTTTTAAGGAGGCCGAAAAGTTCTTCGTCTCCATTTCCTTGCCATACATGACCCAAGGTTTTT GGGATAACTCTATGTTGACTGAGCCAGGTGATGGTAGAAAGGTTGTTTGTCATCCAACTGCTTGGGATTTGGGTAAGGGTGATTTTAGAATTAAGATGTGCAC CAAGGTCACCATGGACGACTTCTTGACTGCTCATCATGAAATGGGTCATATCCAATACGATATGGCCTACGCTGCTCAACCATACTTGTTGAGAAACGGTGCT AACGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCTTTGTCCGCTGCTACTCCACATTACTTGAAGGCTTTGGGTTTGTTGGCTCCAGATTTTCATGAAGAT AACGAGACCGAAATTAACTTCTTGTTGAAGCAGGCCTTGACCATCGTTGGTACTTTGCCATTTACTTACATGTTGGAGAAGTGGAGATGGATGGTTTTTAAGGG TGAAATTCCAAAGCAACAGTGGATGGAAAAGTGGTGGGAAATGAAGAGAGAAATTGTCGGTGTTGTCGAGCCATTGCCACATGATGAAACTTACTGTGATCC AGCTTGTTTGTTTCACGTTGCTGAAGATTACTCCTTTATCAGATACTACACCCGTACCATCTACCAGTTCCAATTTCATGAGGCCTTGTGCAAGACTGCTAAGCA TGAAGGTGCTTTGTTTAAGTGTGATATCTCCAACTCCACTGAGGCCGGTCAAAGATTGTTGCAAATGTTGAGATTGGGTAAGTCCGAACCATGGACTTTGGCTTTGGAAAACATTGTTGGTATTAAGACCATGGACGTCAAGCCATTGTTGAACTACTTTGAGCCATTGTTTACTTGGTTGAAGGAGCAGAACCGTAACTCTTTTGTC GGTTGGTCTACTGAATGGACTCCATACTCTGAT
SEQ ID NO.7:DrACE2-740(斑马鱼)核苷酸序列
CAAACTGTTGAAGATCGTGCTCGTGAATTTTTGAACAAGTTTGATGAGGAAGCTTCCGACATTATGTACCAGTACACCTTGGCTTCTTGGGCTTACAACAC TGATATTTCTCAAGAGAACGCCGACAAGGAAGCTGAAGCTTACGCTATTTGGTCTGAATACTACAACAAGATGTCCGAGGAATCTAACGCTTACCCAATTGAT CAAATTTCCGACCCAATCATCAAGATGCAGTTGCAAAAGTTGCAGGACAAGGGTTCTGGTGCTTTGTCTCCAGATAAGGCTTCTGAATTGAGAAACATTATGT CCGAGATGTCTACCATTTACAACACCGCTACCGTTTGCAAGATTGACGATCCAACTGATTGTCAGACTTTGGAACCAGGTTTGGAATCTATTATGGCCGAATCT AGAGACTACGACGAACGTTTGCATGTTTGGGAAGGTTGGAGAGTTGCTACTGGTATGAAGATGAGACCATTGTACGAAAAGTACGTCGATTTGAAGAACGAG GCTGCTAAGTTGAACAACTACGAAGATCATGGTGATTACTGGAGAGGTGATTACGAAACTATTGACGATCCAAAGTACTCTTACTCCCGTGACCAAGTTATTG AGGATGCTAGAAGAATTTACAAGGAGATATTGCCCTTGTACAAGGAGTTGCACGCTTACGTTAGAGCTAAGTTGCAAGATGTTTACCCAGGTCATATTGGTTC TGATGCTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGATGATCCCATACCCAGATAGACCAGATATTGACG TCTCTTCCGCTATGGTTGAGCAAGGTTGGGATGAAATTAGATTGTTTAAGGAGGCCGAGAAGTTTTTCATGTCTGTTAACATGCCAGCCATGTTCGACAACTTT TGGAACAACTCTATGTTCATCAAGCCAGAGGAACGTGACGTTGTTTGTCATCCAACTGCTTGGGATATGGGTAACAGAAAGGATTTTAGAATCAAGATGTGCA CCAAGGTCAACATGGACGATTTCTTGACTGTCCACCATGAGATGGGTCATAACCAATACCAGATGGCTTACAGAAACCATCCATACTTGTTGAGAGATGGTGC TAACGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCTTTGTCCGCCGCTACTCCATCTCATTTGCAATCTTTGGGTTTGTTGCCATCTGATTTTAAGCAGGA TTACGAAACCGATATCAACTTCTTGTTGAAGCAGGCTTTGACTATCGTTGGTACTTTGCCATTTACTTACATGTTGGAGGAATGGCGTTGGCAGGTTTTTAAGG CTAAGATTCCAAAGGACGAGTGGATGCAACAATGGTGGCAAATGAAGAGAGAATTGGTTGGTGTTGCTGAAGCTGTTCCAAGAGATGAAACTTACTGTGATC CACCAGCTTTGTTTCATGTTTCTGGTGATTACTCTTTCATCCGTTACTTCACCAGAACCATTTACCAGTTCCAATTTCAGGAAGCCTTGTGCAAGGCTGCCGGTC ATACTGGTCCATTGTACAAGTGTGATATTACCAACTCCACCAAGGCTGGTGATAAGTTGAGACATATGTTGGAATTGGGTAGATCCATGTCCTGGACTAGAGC TTTGGAAGAAGTTGCTGGTACTACTAAGATGGATTCTCAACCATTGTTGCACTACTTTTCCACCTTGATGGAGTGGTTGAAGGAAGAGAACCAAAAGAACAAC AGAGTTCCCGGTTGGAACGTTAACGTTAACCCAGGTGTTTTGACTTCTTCTTTTATCAACGACGCCGAAATTTCCGAAAACGCCTTCAAGGTCAGAATTTCTTT GAAGTCTGCTTTGGGTAACGAGGCCTACACTTGGAACGCTAACGATATTTACTTGTTTAAGTCCACCATGGCCTTTGCCATGAGACAATACTACTTGAAGGAG AAGAACACCGATGTTAACTTTACCCCAGAGAACATCCATACTTACAACGAAACTGCTAGAATCTCCTTCAAGTTCGCCGTTATGGACCCAACTAAGACTGGTA CTGTTATTCCAAAGGCTGAAGTTGAAAACGCCATTTGGCAAGAAAGAGATAGAATTAACGGTGCCTTTTTGTTGTCCGACGAAACTTTGGAATTTGTCGGTTTG ATGGCTACCTTGGCTCCACCAAAGGAAGAAAAGATTACT
SEQ ID NO.8:DrACE2-615(斑马鱼)核苷酸序列
CAAACTGTTGAAGATCGTGCTCGTGAATTTTTGAACAAGTTTGATGAGGAAGCTTCCGACATTATGTACCAGTACACCTTGGCTTCTTGGGCTTACA ACACTGATATTTCTCAAGAGAACGCCGACAAGGAAGCTGAAGCTTACGCTATTTGGTCTGAATACTACAACAAGATGTCCGAGGAATCTAACGCTTACCCAAT TGATCAAATTTCCGACCCAATCATCAAGATGCAGTTGCAAAAGTTGCAGGACAAGGGTTCTGGTGCTTTGTCTCCAGATAAGGCTTCTGAATTGAGAAACATT ATGTCCGAGATGTCTACCATTTACAACACCGCTACCGTTTGCAAGATTGACGATCCAACTGATTGTCAGACTTTGGAACCAGGTTTGGAATCTATTATGGCCGA ATCTAGAGACTACGACGAACGTTTGCATGTTTGGGAAGGTTGGAGAGTTGCTACTGGTATGAAGATGAGACCATTGTACGAAAAGTACGTCGATTTGAAGAAC GAGGCTGCTAAGTTGAACAACTACGAAGATCATGGTGATTACTGGAGAGGTGATTACGAAACTATTGACGATCCAAAGTACTCTTACTCCCGTGACCAAGTTA TTGAGGATGCTAGAAGAATTTACAAGGAGATATTGCCCTTGTACAAGGAGTTGCACGCTTACGTTAGAGCTAAGTTGCAAGATGTTTACCCAGGTCATATTGG TTCTGATGCTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGATGATCCCATACCCAGATAGACCAGATATTGACGTCTCTTCCGCTATGGTTGAGCAAGGTTGGGATGAAATTAGATTGTTTAAGGAGGCCGAGAAGTTTTTCATGTCTGTTAACATGCCAGCCATGTTCGACAAC TTTTGGAACAACTCTATGTTCATCAAGCCAGAGGAACGTGACGTTGTTTGTCATCCAACTGCTTGGGATATGGGTAACAGAAAGGATTTTAGAATCAAGATGT GCACCAAGGTCAACATGGACGATTTCTTGACTGTCCACCATGAGATGGGTCATAACCAATACCAGATGGCTTACAGAAACCATCCATACTTGTTGAGAGATGG TGCTAACGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCTTTGTCCGCCGCTACTCCATCTCATTTGCAATCTTTGGGTTTGTTGCCATCTGATTTTAAGCA GGATTACGAAACCGATATCAACTTCTTGTTGAAGCAGGCTTTGACTATCGTTGGTACTTTGCCATTTACTTACATGTTGGAGGAATGGCGTTGGCAGGTTTTTA AGGCTAAGATTCCAAAGGACGAGTGGATGCAACAATGGTGGCAAATGAAGAGAGAATTGGTTGGTGTTGCTGAAGCTGTTCCAAGAGATGAAACTTACTGTG ATCCACCAGCTTTGTTTCATGTTTCTGGTGATTACTCTTTCATCCGTTACTTCACCAGAACCATTTACCAGTTCCAATTTCAGGAAGCCTTGTGCAAGGCTGCCG GTCATACTGGTCCATTGTACAAGTGTGATATTACCAACTCCACCAAGGCTGGTGATAAGTTGAGACATATGTTGGAATTGGGTAGATCCATGTCCTGGACTAGAGCTTTGGAAGAAGTTGCTGGTACTACTAAGATGGATTCTCAACCATTGTTGCACTACTTTTCCACCTTGATGGAGTGGTTGAAGGAAGAGAACCAAAAGAAC AACAGAGTTCCCGGTTGGAACGTTAACGTTAACCCAGGTGTTTTGACTTCTTCTTTTATCAACGACGCCGAAATTTCCGAACACCATCAC
SEQ ID NO.9:dACE2-740(狗)核苷酸序列
TCCACCGAAGATTTGGTTAAGACCTTTTTGGAAAAGTTCAACTACGAGGCCGAAGAGTTGTCTTACCAGTCTTCTTTGGCTTCTTGGAACTACAACATTAA CATCACCGACGAAAACGTCCAAAAGATGAACAACGCCGGTGCTAAGTGGTCTGCTTTTTACGAAGAACAATCTAAGTTGGCCAAGACCTACCCATTGGAAGA AATTCAAGATTCCACCGTCAAGCGTCAGTTGCGTGCTTTGCAACATTCTGGTTCTTCTGTTTTGTCTGCCGATAAGAACCAACGTTTGAACACTATTTTGAACTC CATGTCCACCGTTTACTCTACCGGTAAGGCCTGTAACCCATCTAACCCACAAGAATGTTTGTTGTTGGAACCAGGTTTGGATGATATTATGGAGAACTCTAAGG ACTACAACGAGCGTTTGTGGGCTTGGGAAGGTTGGCGTTCTGAAGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTCGCTTTGAAGAACGAGATGGC TAGAGCTAACAACTACGAAGATTACGGTGATTACTGGAGAGGTGATTACGAAGAAGAATGGGAAAACGGTTACAACTACTCTAGAAACCAATTGATCGACGA TGTCGAATTGACCTTTACCCAGATCATGCCATTGTACCAACATTTGCACGCTTACGTTAGAACTAAGTTGATGGATACTTACCCATCCTACATCTCCCCAACTG GTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGACTGTCCCATTTGGTCAAAAGCCAAACATTGACGTCACC AACGCTATGGTTAACCAATCTTGGGATGCTAGAAAGATTTTCAAGGAGGCCGAGAAGTTCTTCGTCTCTGTCGGTTTGCCAAACATGACTCAAGAATTTTGGG GTAACTCTATGTTGACCGAACCATCTGATTCTAGAAAGGTTGTTTGTCACCCAACTGCTTGGGATTTGGGTAAGGGTGATTTTAGAATTAAGATGTGCACCAAG GTCACCATGGACGATTTTTTGACTGCTCACCACGAGATGGGTCATATTCAATACGATATGGCTTACGCCGCTCAACCATTTTTGTTGAGAAACGGTGCTAACGA AGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCTGCTACTCCAAACCATTTGAAGAACATTGGTTTGTTGCCACCATCTTTTTTCGAGGACTCTGA AACTGAAATTAACTTCTTGTTGAAGCAGGCCTTGACCATTGTCGGTACTTTGCCATTTACCTACATGTTGGAAAAGTGGAGATGGATGGTTTTTAAGGGTGAAA TTCCCAAGGACCAGTGGATGAAGACTTGGTGGGAAATGAAGAGAAACATTGTCGGTGTTGTCGAACCAGTTCCACATGATGAAACTTACTGTGATCCAGCTTC TTTGTTTCACGTTGCTAACGATTACTCCTTTATCCGTTACTACACTCGTACTATCTACCAGTTCCAATTCCAGGAGGCCTTGTGCCAGATCGCCAAGCATGAAGG TCCATTGCATAAGTGTGATATTTCCAACTCCTCTGAGGCCGGTCAAAAGTTGTTGGAAATGTTGAAGTTGGGTAAGTCTAAGCCATGGACTTACGCTTTGGAAA TTGTTGTTGGTGCTAAGAACATGGACGTCAGACCATTGTTGAACTACTTCGAACCATTGTTTACCTGGTTGAAGGAGCAGAACAGAAACTCCTTTGTCGGTTGG AACACTGATTGGTCTCCATACGCTGATCAATCCATTAAGGTTCGTATCTCCTTGAAGTCTGCCTTGGGTGAAAAGGCTTACGAATGGAACAACAACGAAATGT ACTTGTTCCGTTCTTCCATCGCCTACGCCATGCGTCAATACTTTTCTGAAGTTAAGAACCAGACCATCCCCTTCGTTGAAGACAACGTTTGGGTTTCTGATTTGA AGCCAAGAATTTCCTTCAACTTCTCCGTCACCTCCCCAGGTAACGTCTCTGATATTATTCCAAGAACTGAGGTCGAAGAGGCTATCAGAATGTACCGTTCTAGA ATCAACGACGTCTTCAGATTGGATGACAACTCCTTGGAATTTTTGGGCATCCAACCAACTCCAGGTCCACCATACGAACCACCAGTTACT
SEQ ID NO.10:dACE2-615(狗)核苷酸序列
TCCACCGAAGATTTGGTTAAGACCTTTTTGGAAAAGTTCAACTACGAGGCCGAAGAGTTGTCTTACCAGTCTTCTTTGGCTTCTTGGAACTACAACA TTAACATCACCGACGAAAACGTCCAAAAGATGAACAACGCCGGTGCTAAGTGGTCTGCTTTTTACGAAGAACAATCTAAGTTGGCCAAGACCTACCCATTGGA AGAAATTCAAGATTCCACCGTCAAGCGTCAGTTGCGTGCTTTGCAACATTCTGGTTCTTCTGTTTTGTCTGCCGATAAGAACCAACGTTTGAACACTATTTTGA ACTCCATGTCCACCGTTTACTCTACCGGTAAGGCCTGTAACCCATCTAACCCACAAGAATGTTTGTTGTTGGAACCAGGTTTGGATGATATTATGGAGAACTCT AAGGACTACAACGAGCGTTTGTGGGCTTGGGAAGGTTGGCGTTCTGAAGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTCGCTTTGAAGAACGAG ATGGCTAGAGCTAACAACTACGAAGATTACGGTGATTACTGGAGAGGTGATTACGAAGAAGAATGGGAAAACGGTTACAACTACTCTAGAAACCAATTGATC GACGATGTCGAATTGACCTTTACCCAGATCATGCCATTGTACCAACATTTGCACGCTTACGTTAGAACTAAGTTGATGGATACTTACCCATCCTACATCTCCCC AACTGGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGACTGTCCCATTTGGTCAAAAGCCAAACATTGACGTCACCAACGCTATGGTTAACCAATCTTGGGATGCTAGAAAGATTTTCAAGGAGGCCGAGAAGTTCTTCGTCTCTGTCGGTTTGCCAAACATGACTCAAGAATTT TGGGGTAACTCTATGTTGACCGAACCATCTGATTCTAGAAAGGTTGTTTGTCACCCAACTGCTTGGGATTTGGGTAAGGGTGATTTTAGAATTAAGATGTGCAC CAAGGTCACCATGGACGATTTTTTGACTGCTCACCACGAGATGGGTCATATTCAATACGATATGGCTTACGCCGCTCAACCATTTTTGTTGAGAAACGGTGCTA ACGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCTGCTACTCCAAACCATTTGAAGAACATTGGTTTGTTGCCACCATCTTTTTTCGAGGACT CTGAAACTGAAATTAACTTCTTGTTGAAGCAGGCCTTGACCATTGTCGGTACTTTGCCATTTACCTACATGTTGGAAAAGTGGAGATGGATGGTTTTTAAGGGT GAAATTCCCAAGGACCAGTGGATGAAGACTTGGTGGGAAATGAAGAGAAACATTGTCGGTGTTGTCGAACCAGTTCCACATGATGAAACTTACTGTGATCCA GCTTCTTTGTTTCACGTTGCTAACGATTACTCCTTTATCCGTTACTACACTCGTACTATCTACCAGTTCCAATTCCAGGAGGCCTTGTGCCAGATCGCCAAGCAT GAAGGTCCATTGCATAAGTGTGATATTTCCAACTCCTCTGAGGCCGGTCAAAAGTTGTTGGAAATGTTGAAGTTGGGTAAGTCTAAGCCATGGACTTACGCTTT GGAAATTGTTGTTGGTGCTAAGAACATGGACGTCAGACCATTGTTGAACTACTTCGAACCATTGTTTACCTGGTTGAAGGAGCAGAACAGAAACTCCTTTGTC GGTTGGAACACTGATTGGTCTCCATACGCTGAT
SEQ ID NO.11:DcACE2-740(猫)核苷酸序列
TCCACTACTGAGGAATTGGCTAAGACTTTTTTGGAGAAGTTTAACCACGAGGCCGAAGAGTTGTCCTACCAATCTTCTTTGGCTTCTTGGAACTACAACAC TAACATTACCGACGAGAACGTCCAGAAGATGAACGAAGCTGGTGCTAAGTGGTCTGCTTTTTACGAAGAACAATCTAAGTTGGCCAAGACCTACCCATTGGCT GAAATTCATAACACCACTGTCAAGAGACAATTGCAGGCTTTGCAACAATCTGGTTCTTCTGTTTTGTCTGCTGATAAGTCTCAACGTTTGAACACTATCTTGAA CGCCATGTCTACTATTTACTCCACTGGTAAGGCTTGTAACCCAAACAACCCACAAGAATGTTTGTTGTTGGAACCAGGTTTGGATGATATTATGGAGAACTCTA AGGACTACAACGAGCGTTTGTGGGCTTGGGAAGGTTGGAGAGCTGAAGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTTGCCTTGAAGAACGAAA TGGCTAGAGCTAACAACTACGAAGATTACGGTGATTACTGGAGAGGTGATTACGAAGAAGAATGGACTGATGGTTACAACTACTCTCGTTCTCAATTGATCAA GGACGTCGAACACACCTTCACTCAAATCAAGCCATTGTACCAACATTTGCACGCCTACGTTAGAGCCAAGTTGATGGATACTTACCCATCTAGAATTTCCCCCA CCGGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGACTGTCCCATTTGGTCAAAAGCCAAACATTGATGTT ACCGACGCCATGGTTAACCAATCTTGGGATGCTAGAAGAATTTTCAAGGAGGCCGAAAAGTTCTTTGTTTCCGTTGGTTTGCCAAACATGACTCAGGGTTTTTG GGAAAACTCTATGTTGACTGAGCCAGGTGATTCTAGAAAGGTTGTTTGTCATCCAACTGCCTGGGATTTGGGTAAGGGTGATTTTAGAATTAAGATGTGCACC AAGGTCACCATGGACGATTTTTTGACTGCCCATCATGAGATGGGTCACATTCAATACGATATGGCTTACGCTGTTCAACCATTTTTGTTGAGAAACGGTGCTAA CGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCCGCTACTCCAAACCATTTGAAGACTATTGGTTTGTTGTCCCCAGGTTTTTCCGAAGACTC TGAAACTGAAATTAACTTCTTGTTGAAGCAGGCCTTGACCATCGTTGGTACCTTGCCATTTACCTACATGTTGGAAAAGTGGAGATGGATGGTTTTTAAGGGTG AAATTCCCAAGGAACAATGGATGCAAAAGTGGTGGGAAATGAAGAGAGAAATTGTCGGTGTTGTCGAGCCAGTTCCACATGATGAAACTTACTGTGATCCAG CTTCTTTGTTTCACGTCGCTAACGATTACTCTTTTATCAGATACTACACCCGTACCATCTACCAGTTTCAGTTTCAGGAGGCTTTGTGTAGAATCGCTAAGCATG AAGGTCCATTGCATAAGTGTGATATTTCCAACTCTTCCGAGGCCGGTAAGAAGTTGTTGCAAATGTTGACTTTGGGTAAGTCTAAGCCATGGACTTTGGCTTTG GAACATGTTGTTGGTGAAAAGAAGATGAACGTCACCCCATTGTTGAAGTACTTTGAGCCATTGTTTACCTGGTTGAAGGAGCAAAACAGAAACTCTTTTGTCG GCTGGAACACCGATTGGAGACCATACGCTGATCAGTCTATCAAGGTCAGAATTTCCTTGAAGTCCGCCTTGGGTGACGAAGCTTACGAATGGAACGATAACGA AATGTACTTGTTCCGTTCTTCCGTTGCCTACGCTATGCGTGAGTACTTTTCTAAGGTTAAGAACCAGACTATTCCATTCGTCGAGGATAACGTCTGGGTTTCTAA CTTGAAGCCAAGAATTTCTTTCAACTTCTTCGTCACCGCTTCCAAGAACGTTTCCGATGTTATTCCAAGATCCGAAGTTGAAGAAGCCATTCGTATGTCTAGAT CCAGAATCAACGACGCTTTTAGATTGGACGACAACTCCTTGGAATTTTTGGGTATTCAACCAACTTTGTCCCCACCATACCAACCACCAGTTACT
SEQ ID NO.12:DcACE2-615(猫)核苷酸序列
TCCACTACTGAGGAATTGGCTAAGACTTTTTTGGAGAAGTTTAACCACGAGGCCGAAGAGTTGTCCTACCAATCTTCTTTGGCTTCTTGGAACTACA ACACTAACATTACCGACGAGAACGTCCAGAAGATGAACGAAGCTGGTGCTAAGTGGTCTGCTTTTTACGAAGAACAATCTAAGTTGGCCAAGACCTACCCATT GGCTGAAATTCATAACACCACTGTCAAGAGACAATTGCAGGCTTTGCAACAATCTGGTTCTTCTGTTTTGTCTGCTGATAAGTCTCAACGTTTGAACACTATCT TGAACGCCATGTCTACTATTTACTCCACTGGTAAGGCTTGTAACCCAAACAACCCACAAGAATGTTTGTTGTTGGAACCAGGTTTGGATGATATTATGGAGAAC TCTAAGGACTACAACGAGCGTTTGTGGGCTTGGGAAGGTTGGAGAGCTGAAGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTTGCCTTGAAGAAC GAAATGGCTAGAGCTAACAACTACGAAGATTACGGTGATTACTGGAGAGGTGATTACGAAGAAGAATGGACTGATGGTTACAACTACTCTCGTTCTCAATTGA TCAAGGACGTCGAACACACCTTCACTCAAATCAAGCCATTGTACCAACATTTGCACGCCTACGTTAGAGCCAAGTTGATGGATACTTACCCATCTAGAATTTCC CCCACCGGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGACTGTCCCATTTGGTCAAAAGCCAAACATTGATGTTACCGACGCCATGGTTAACCAATCTTGGGATGCTAGAAGAATTTTCAAGGAGGCCGAAAAGTTCTTTGTTTCCGTTGGTTTGCCAAACATGACTCAGGGTT TTTGGGAAAACTCTATGTTGACTGAGCCAGGTGATTCTAGAAAGGTTGTTTGTCATCCAACTGCCTGGGATTTGGGTAAGGGTGATTTTAGAATTAAGATGTGC ACCAAGGTCACCATGGACGATTTTTTGACTGCCCATCATGAGATGGGTCACATTCAATACGATATGGCTTACGCTGTTCAACCATTTTTGTTGAGAAACGGTGC TAACGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCCGCTACTCCAAACCATTTGAAGACTATTGGTTTGTTGTCCCCAGGTTTTTCCGAAGA CTCTGAAACTGAAATTAACTTCTTGTTGAAGCAGGCCTTGACCATCGTTGGTACCTTGCCATTTACCTACATGTTGGAAAAGTGGAGATGGATGGTTTTTAAGG GTGAAATTCCCAAGGAACAATGGATGCAAAAGTGGTGGGAAATGAAGAGAGAAATTGTCGGTGTTGTCGAGCCAGTTCCACATGATGAAACTTACTGTGATC CAGCTTCTTTGTTTCACGTCGCTAACGATTACTCTTTTATCAGATACTACACCCGTACCATCTACCAGTTTCAGTTTCAGGAGGCTTTGTGTAGAATCGCTAAGC ATGAAGGTCCATTGCATAAGTGTGATATTTCCAACTCTTCCGAGGCCGGTAAGAAGTTGTTGCAAATGTTGACTTTGGGTAAGTCTAAGCCATGGACTTTGGCT TTGGAACATGTTGTTGGTGAAAAGAAGATGAACGTCACCCCATTGTTGAAGTACTTTGAGCCATTGTTTACCTGGTTGAAGGAGCAAAACAGAAACTCTTTTG TCGGCTGGAACACCGATTGGAGACCATACGCTGAT
SEQ ID NO.13:DfACE2-740(雪貂)核苷酸序列
TCTACTACCGAAGATTTGGCTAAGACTTTCTTGGAAAAGTTCAACTACGAGGCCGAAGAATTGTCTTACCAAAACTCTTTGGCTTCCTGGAACTACAACAC TAACATTACTGATGAGAACATCCAGAAGATGAACATCGCCGGTGCCAAGTGGTCTGCTTTTTACGAAGAAGAATCTCAGCATGCCAAGACCTACCCATTGGAA GAAATTCAGGACCCAATTATTAAGCGTCAGTTGAGAGCCTTGCAACAGTCTGGTTCTTCTGTTTTGTCTGCTGATAAGAGAGAACGCTTGAACACTATTTTGAA CGCCATGTCCACTATCTACTCCACTGGTAAGGCTTGTAACCCAAACAACCCACAAGAATGTTTGTTGTTGGAACCAGGTTTGGATGATATTATGGAAAACTCCA AGGACTACAACGAGCGTTTGTGGGCTTGGGAAGGTTGGCGTTCTGAAGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTCGCTTTGAAGAACGAAA TGGCCAGAGCTAACAACTACGAAGATTACGGTGATTACTGGAGAGGTGATTACGAAGAAGAATGGGCTGATGGTTACTCTTACTCTAGAAACCAATTGATCG AGGACGTCGAGCATACTTTTACTCAAATCAAGCCATTGTACGAGCACTTGCACGCTTACGTTAGAGCTAAGTTGATGGATGCTTACCCATCTAGAATTTCCCCA ACTGGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGATGGTCCCATTTAGACAGAAGCCAAACATTGACGT TACTGACGCTATGGTTAACCAATCTTGGGATGCTAGAAGAATTTTCGAGGAGGCTGAAACCTTTTTTGTTTCCGTTGGTTTGCCAAACATGACCGAAGGTTTTT GGCAAAACTCTATGTTGACTGAGCCAGGTGATAACAGAAAGGTTGTTTGTCATCCAACTGCCTGGGATTTGGGTAAGAGAGATTTTAGAATTAAGATGTGCAC CAAGGTCACCATGGACGACTTCTTGACTGCTCATCATGAAATGGGTCATATTCAATACGACATGGCCTACGCTGAACAACCATTTTTGTTGAGAAACGGTGCT AACGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCCGCTACTCCAAACCATTTGAAGAACATTGGTTTGTTGCCCCCAGATTTTTCCGAAGA CTCTGAAACTGACATTAACTTCTTGTTGAAGCAAGCCTTGACCATCGTTGGTACTTTGCCATTTACTTACATGTTGGAGAAGTGGCGTTGGATGGTTTTTAAGG GTGAAATTCCAAAGGAGCAGTGGATGCAAAAGTGGTGGGAAATGAAGAGAGATATTGTCGGTGTTGTTGAGCCATTGCCACATGATGAAACTTACTGTGATC CAGCTGCTTTGTTTCATGTTGCTAACGATTACTCTTTCATCCGTTACTACACCCGTACTATCTACCAGTTTCAATTTCAGGAAGCCTTGTGTCAAATTGCCAAGC ACGAAGGTCCATTGTACAAGTGTGATATTTCTAACTCCTCCGAGGCCGGTCAAAAGTTGCATGAAATGTTGTCTTTGGGTCGTTCTAAGCCATGGACTTTTGCT TTGGAAAGAGTTGTTGGTGCTAAGACTATGGATGTTAGACCATTGTTGAACTACTTCGAGCCATTGTTTACTTGGTTGAAGGAGCAGAACAGAAACTCCTTCGT CGGTTGGAACACTGATTGGTCTCCATACGCTGATCAATCCATTAAGGTCCGTATCTCTTTGAAGTCTGCTTTGGGTGAAAAGGCTTACGAATGGAACGATAACG AAATGTACTTTTTCCAGTCCTCCATCGCTTACGCTATGAGAGAATACTTTTCCAAGGTCAAGAACCAGACTATTCCATTTGTTGGTAAGGACGTTAGAGTCTCC GATTTGAAGCCAAGAATTTCCTTTAACTTCATCGTCACCTCCCCAGAGAACATGTCTGATATTATTCCAAGAGCCGATGTCGAAGAGGCCATTCGTAAGTCTAG AGGTAGAATTAACGATGCCTTTCGTTTGGACGATAACTCCTTGGAATTTTTGGGTATCCAGCCAACCTTGGAGCCACCATACCAACCACCAGTTACT
SEQ ID NO.14:DfACE2-615(雪貂)核苷酸序列
TCTACTACCGAAGATTTGGCTAAGACTTTCTTGGAAAAGTTCAACTACGAGGCCGAAGAATTGTCTTACCAAAACTCTTTGGCTTCCTGGAACTACA ACACTAACATTACTGATGAGAACATCCAGAAGATGAACATCGCCGGTGCCAAGTGGTCTGCTTTTTACGAAGAAGAATCTCAGCATGCCAAGACCTACCCATT GGAAGAAATTCAGGACCCAATTATTAAGCGTCAGTTGAGAGCCTTGCAACAGTCTGGTTCTTCTGTTTTGTCTGCTGATAAGAGAGAACGCTTGAACACTATTT TGAACGCCATGTCCACTATCTACTCCACTGGTAAGGCTTGTAACCCAAACAACCCACAAGAATGTTTGTTGTTGGAACCAGGTTTGGATGATATTATGGAAAA CTCCAAGGACTACAACGAGCGTTTGTGGGCTTGGGAAGGTTGGCGTTCTGAAGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTCGCTTTGAAGAAC GAAATGGCCAGAGCTAACAACTACGAAGATTACGGTGATTACTGGAGAGGTGATTACGAAGAAGAATGGGCTGATGGTTACTCTTACTCTAGAAACCAATTG ATCGAGGACGTCGAGCATACTTTTACTCAAATCAAGCCATTGTACGAGCACTTGCACGCTTACGTTAGAGCTAAGTTGATGGATGCTTACCCATCTAGAATTTC CCCAACTGGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGATGGTCCCATTTAGACAGAAGCCAAACATTGACGTTACTGACGCTATGGTTAACCAATCTTGGGATGCTAGAAGAATTTTCGAGGAGGCTGAAACCTTTTTTGTTTCCGTTGGTTTGCCAAACATGACCGAAGGT TTTTGGCAAAACTCTATGTTGACTGAGCCAGGTGATAACAGAAAGGTTGTTTGTCATCCAACTGCCTGGGATTTGGGTAAGAGAGATTTTAGAATTAAGATGT GCACCAAGGTCACCATGGACGACTTCTTGACTGCTCATCATGAAATGGGTCATATTCAATACGACATGGCCTACGCTGAACAACCATTTTTGTTGAGAAACGG TGCTAACGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCCGCTACTCCAAACCATTTGAAGAACATTGGTTTGTTGCCCCCAGATTTTTCCGA AGACTCTGAAACTGACATTAACTTCTTGTTGAAGCAAGCCTTGACCATCGTTGGTACTTTGCCATTTACTTACATGTTGGAGAAGTGGCGTTGGATGGTTTTTA AGGGTGAAATTCCAAAGGAGCAGTGGATGCAAAAGTGGTGGGAAATGAAGAGAGATATTGTCGGTGTTGTTGAGCCATTGCCACATGATGAAACTTACTGTG ATCCAGCTGCTTTGTTTCATGTTGCTAACGATTACTCTTTCATCCGTTACTACACCCGTACTATCTACCAGTTTCAATTTCAGGAAGCCTTGTGTCAAATTGCCA AGCACGAAGGTCCATTGTACAAGTGTGATATTTCTAACTCCTCCGAGGCCGGTCAAAAGTTGCATGAAATGTTGTCTTTGGGTCGTTCTAAGCCATGGACTTTTGCTTTGGAAAGAGTTGTTGGTGCTAAGACTATGGATGTTAGACCATTGTTGAACTACTTCGAGCCATTGTTTACTTGGTTGAAGGAGCAGAACAGAAACTCCTT CGTCGGTTGGAACACTGATTGGTCTCCATACGCTGAT
SEQ ID NO.15:MmACE2-740(恒河猴)核苷酸序列
TCTACCATTGAAGAACAGGCTAAGACTTTCTTGGATAAGTTTAACCACGAAGCCGAGGATTTGTTTTACCAGTCCTCTTTGGCTTCCTGGAACTACAACAC TAACATTACTGAAGAGAACGTCCAGAACATGAACAACGCTGGTGAAAAGTGGTCTGCTTTTTTGAAGGAACAATCCACCTTGGCCCAAATGTACCCATTGCAA GAAATTCAAAACTTGACTGTCAAGTTGCAGTTGCAGGCTTTGCAACAAAACGGTTCTTCTGTTTTGTCTGAGGATAAGTCTAAGCGTTTGAACACCATTTTGAA CACTATGTCTACCATCTACTCCACCGGTAAGGTCTGCAACCCAAACAACCCACAAGAATGTTTGTTGTTGGACCCAGGTTTGAACGAAATTATGGAGAAGTCC TTGGACTACAACGAGCGTTTGTGGGCCTGGGAAGGTTGGAGATCCGAAGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTCGTTTTGAAGAACGAG ATGGCCGGTGCTAACCATTACAAGGATTACGGTGATTACTGGAGAGGTGATTACGAAGTTAACGGTGTTGATGGTTACGATAACAACAGAGATCAATTGATCG AGGACGTCGAGAGAACTTTCGAAGAGATCAAGCCATTGTACGAGCATTTGCATGCTTACGTTAGAGCTAAGTTGATGAACGCTTACCCATCTTACATTTCCCC AACTGGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACTCTTTGACCGTCCCATTTGGTCAAAAGCCAAACATTGATGT CACTGACGCTATGGTTAACCAAGCTTGGAACGCTCAAAGAATTTTTAAGGAGGCCGAAAAGTTTTTCGTCTCCGTCGGTTTGCCAAACATGACTCAAGGTTTTT GGGAAAACTCTATGTTGACCGATCCAGGTAACGTTCAAAAGGTTGTTTGTCATCCAACTGCCTGGGATTTGGGTAAGGGTGATTTTAGAATTATCATGTGCACC AAGGTCACCATGGATGACTTTTTGACTGCTCATCATGAAATGGGTCATATCCAGTACGATATGGCCTACGCTGCTCAACCATTTTTGTTGAGAAACGGTGCTAA CGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCCGCTGCTACTCCAAAGCATTTGAAGTCTATTGGTTTGTTGTCCCCCGACTTCCAGGAGGATA ACGAAACTGAGATTAACTTCTTGTTGAAGCAGGCCTTGACTATCGTTGGTACTTTGCCATTTACTTACATGTTGGAGAAGTGGAGATGGATGGTTTTTAAGGGT GAAATTCCAAAGGACCAGTGGATGAAGAAGTGGTGGGAAATGAAGAGAGAAATTGTCGGTGTTGTCGAGCCAGTCCCACATGATGAAACTTACTGTGATCCA GCTTCTTTGTTTCATGTCTCTAACGATTACTCCTTCATCCGCTACTACACTCGTACTTTGTACCAGTTCCAGTTTCAGGAGGCTTTGTGCCAAGCTGCTAAGCAT GAAGGTCCATTGCATAAGTGTGATATTTCCAACTCTACCGAGGCCGGTCAAAAGTTGTTGAACATGTTGAAGTTGGGTGAGTCCGAACCATGGACTTTGGCTTT GGAAAACGTTGTTGGTGCTAAGAACATGAACGTTAGACCATTGTTGAACTACTTCGAGCCATTGTTCACTTGGTTGAAGGATCAGAACAAGAACTCTTTTGTC GGTTGGTCTACTGACTGGTCTCCATACGCTGATCAATCCATTAAGGTCAGAATCTCCTTGAAGTCTGCTTTGGGTGATAAGGCTTACGAATGGAACGATAACGA AATGTACTTGTTCCGTTCCTCCGTTGCTTACGCTATGAGAACCTACTTTTTGGAAATTAAGCACCAGACCATCTTGTTCGGTGAGGAAGACGTTAGAGTTGCTG ACTTGAAGCCAAGAATTTCTTTTAACTTCTACGTCACTGCCCCCAAGAACGTCTCTGATATTATTCCACGTACTGAGGTTGAAGAAGCCATCAGAATTTCCCGT TCCCGTATTAACGATGCTTTCAGATTGAACGATAACTCCTTGGAGTTTTTGGGTATCCAAACCACTTTGGCTCCACCATACCAATCTCCAGTTACT
SEQ ID NO.16:MmACE2-615(恒河猴)核苷酸序列
TCTACCATTGAAGAACAGGCTAAGACTTTCTTGGATAAGTTTAACCACGAAGCCGAGGATTTGTTTTACCAGTCCTCTTTGGCTTCCTGGAACTACA ACACTAACATTACTGAAGAGAACGTCCAGAACATGAACAACGCTGGTGAAAAGTGGTCTGCTTTTTTGAAGGAACAATCCACCTTGGCCCAAATGTACCCATT GCAAGAAATTCAAAACTTGACTGTCAAGTTGCAGTTGCAGGCTTTGCAACAAAACGGTTCTTCTGTTTTGTCTGAGGATAAGTCTAAGCGTTTGAACACCATTT TGAACACTATGTCTACCATCTACTCCACCGGTAAGGTCTGCAACCCAAACAACCCACAAGAATGTTTGTTGTTGGACCCAGGTTTGAACGAAATTATGGAGAA GTCCTTGGACTACAACGAGCGTTTGTGGGCCTGGGAAGGTTGGAGATCCGAAGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTCGTTTTGAAGAAC GAGATGGCCGGTGCTAACCATTACAAGGATTACGGTGATTACTGGAGAGGTGATTACGAAGTTAACGGTGTTGATGGTTACGATAACAACAGAGATCAATTG ATCGAGGACGTCGAGAGAACTTTCGAAGAGATCAAGCCATTGTACGAGCATTTGCATGCTTACGTTAGAGCTAAGTTGATGAACGCTTACCCATCTTACATTT CCCCAACTGGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACTCTTTGACCGTCCCATTTGGTCAAAAGCCAAACATTGATGTCACTGACGCTATGGTTAACCAAGCTTGGAACGCTCAAAGAATTTTTAAGGAGGCCGAAAAGTTTTTCGTCTCCGTCGGTTTGCCAAACATGACTCAAGG TTTTTGGGAAAACTCTATGTTGACCGATCCAGGTAACGTTCAAAAGGTTGTTTGTCATCCAACTGCCTGGGATTTGGGTAAGGGTGATTTTAGAATTATCATGT GCACCAAGGTCACCATGGATGACTTTTTGACTGCTCATCATGAAATGGGTCATATCCAGTACGATATGGCCTACGCTGCTCAACCATTTTTGTTGAGAAACGGT GCTAACGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCCGCTGCTACTCCAAAGCATTTGAAGTCTATTGGTTTGTTGTCCCCCGACTTCCAGGA GGATAACGAAACTGAGATTAACTTCTTGTTGAAGCAGGCCTTGACTATCGTTGGTACTTTGCCATTTACTTACATGTTGGAGAAGTGGAGATGGATGGTTTTTA AGGGTGAAATTCCAAAGGACCAGTGGATGAAGAAGTGGTGGGAAATGAAGAGAGAAATTGTCGGTGTTGTCGAGCCAGTCCCACATGATGAAACTTACTGTG ATCCAGCTTCTTTGTTTCATGTCTCTAACGATTACTCCTTCATCCGCTACTACACTCGTACTTTGTACCAGTTCCAGTTTCAGGAGGCTTTGTGCCAAGCTGCTA AGCATGAAGGTCCATTGCATAAGTGTGATATTTCCAACTCTACCGAGGCCGGTCAAAAGTTGTTGAACATGTTGAAGTTGGGTGAGTCCGAACCATGGACTTTGGCTTTGGAAAACGTTGTTGGTGCTAAGAACATGAACGTTAGACCATTGTTGAACTACTTCGAGCCATTGTTCACTTGGTTGAAGGATCAGAACAAGAACTCT TTTGTCGGTTGGTCTACTGACTGGTCTCCATACGCTGAT
SEQ ID NO.17:MjACE2-740(穿山甲)核苷酸序列
TCCACTTCTGATGAAGAAGCTAAGACCTTTTTGGAGAAGTTTAACTCCGAAGCCGAAGAATTGTCCTACCAGTCTTCTTTGGCTTCTTGGAACTACAACAC TAACATTACCGATGAGAACGTCCAGAAGATGAACGTCGCTGGTGCTAAGTGGTCTACTTTTTACGAAGAACAATCCAAGATCGCCAAGAACTACCAGTTGCAG AACATTCAGAACGATACTATTAAGCGTCAGTTGCAGGCTTTGCAATTGTCTGGTTCTTCTGCTTTGTCTGCTGATAAGAACCAAAGATTGAACACCATTTTGAA CACCATGTCCACTATCTACTCTACCGGTAAGGTCTGTAACCCAGGTAACCCACAAGAATGTTCTTTGTTGGAACCAGGTTTGGATAACATTATGGAGTCCTCTA AGGATTACAACGAGCGTTTGTGGGCTTGGGAAGGTTGGCGTTCTGAAGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTCGTCTTGAAGAACGAAAT GGCCAGAGCTAACCATTACGAAGATTACGGTGATTACTGGAGAGGTGATTACGAAGCTGAAGGTGCTAACGGTTACAACTACTCTAGAGATCATTTGATCGAG GACGTCGAACACATTTTTACCCAGATCAAGCCATTGTACGAGCATTTGCATGCTTACGTTAGAGCTAAGTTGATGGATAACTACCCCTCTCATATTTCCCCAAC CGGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGACTGTCCCATTTCGTCAGAAGCCAAACATTGATGTCA CTGATGCTATGGTTAACCAGACTTGGGATGCTAACAGAATTTTTAAGGAGGCCGAGAAGTTCTTTGTCTCCGTCGGTTTGCCAAAGATGACCCAAACTTTTTGG GAAAACTCTATGTTGACCGAGCCAGGTGATGGTAGAAAGGTTGTTTGTCATCCAACTGCTTGGGATTTGGGTAAGCATGATTTTAGAATTAAGATGTGCACCA AGGTCACCATGGACGATTTCTTGACCGCCCATCATGAAATGGGTCATATTCAATACGATATGGCCTACGCTATGCAACCATACTTGTTGAGAAACGGTGCTAA CGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCTGCTACTCCAAAGCATTTGAAGAACATTGGTTTGTTGCCACCAGATTTTTACGAGGACA ACGAAACTGAAATCAACTTCTTGTTGAAGCAGGCCTTGACCATTGTCGGTACTTTGCCATTTACTTACATGCTGGAAAAGTGGCGTTGGATGGTTTTTTCCGGT CAAATTCCAAAGGAGCAGTGGATGAAGAAGTGGTGGGAAATGAAGAGAGAAATTGTCGGTGTTGTTGAGCCAGTTCCACATGATGAAACTTACTGTGATCCA GCTTCTTTGTTTCACGTTGCTAACGATTACTCCTTTATCCGTTACTACACCCGTACTATTTACCAGTTTCAGTTTCAGGAGGCCTTGTGCCAAACCGCCAAGCAT GAAGGTCCATTGCATAAGTGTGATATTTCCAACTCCGCCGAAGCCGGTCAAAAGTTGTTGCAAATGTTGTCTTTGGGTAAGTCCAAGCCATGGACTTTGGCTTT GGAAAGAGTTGTTGGTACTAAGAACATGGACGTTAGACCATTGTTGAACTACTTTGAGCCATTGTTGACTTGGTTGAAGGAACAAAACAAGAACTCCTTTGTC GGTTGGAACACTGATTGGTCTCCATACGCTGCTCAGTCCATCAAGGTCAGAATTTCTTTGAAGTCCGCTTTGGGTGAAAAGGCCTACGAATGGAACGATTCTG AAATGTACTTGTTCCGTTCCTCCGTCGCCTACGCTATGAGAGAATACTTTTCTAAGGTTAAGAAGCAGACCATCCCATTTGAGGATGAGTGTGTTCGTGTCTCC GATTTGAAGCCAAGAGTTTCTTTTATTTTCTTCGTCACCTTGCCCAAGAACGTCTCCGCCGTTATTCCAAGAGCTGAAGTTGAAGAAGCTATTCGTATTTCTCGT TCCAGAATCAACGACGCCTTCAGATTGGACGATAACTCTTTGGAGTTTTTGGGTATTCAGCCCACCTTGCAACCACCATACCAACCACCAGTTACT
SEQ ID NO.18:MjACE2-615(穿山甲)核苷酸序列
TCCACTTCTGATGAAGAAGCTAAGACCTTTTTGGAGAAGTTTAACTCCGAAGCCGAAGAATTGTCCTACCAGTCTTCTTTGGCTTCTTGGAACTACA ACACTAACATTACCGATGAGAACGTCCAGAAGATGAACGTCGCTGGTGCTAAGTGGTCTACTTTTTACGAAGAACAATCCAAGATCGCCAAGAACTACCAGTT GCAGAACATTCAGAACGATACTATTAAGCGTCAGTTGCAGGCTTTGCAATTGTCTGGTTCTTCTGCTTTGTCTGCTGATAAGAACCAAAGATTGAACACCATTT TGAACACCATGTCCACTATCTACTCTACCGGTAAGGTCTGTAACCCAGGTAACCCACAAGAATGTTCTTTGTTGGAACCAGGTTTGGATAACATTATGGAGTCC TCTAAGGATTACAACGAGCGTTTGTGGGCTTGGGAAGGTTGGCGTTCTGAAGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTCGTCTTGAAGAACG AAATGGCCAGAGCTAACCATTACGAAGATTACGGTGATTACTGGAGAGGTGATTACGAAGCTGAAGGTGCTAACGGTTACAACTACTCTAGAGATCATTTGAT CGAGGACGTCGAACACATTTTTACCCAGATCAAGCCATTGTACGAGCATTTGCATGCTTACGTTAGAGCTAAGTTGATGGATAACTACCCCTCTCATATTTCCC CAACCGGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGACTGTCCCATTTCGTCAGAAGCCAAACATTGATGTCACTGATGCTATGGTTAACCAGACTTGGGATGCTAACAGAATTTTTAAGGAGGCCGAGAAGTTCTTTGTCTCCGTCGGTTTGCCAAAGATGACCCAAACTTT TTGGGAAAACTCTATGTTGACCGAGCCAGGTGATGGTAGAAAGGTTGTTTGTCATCCAACTGCTTGGGATTTGGGTAAGCATGATTTTAGAATTAAGATGTGC ACCAAGGTCACCATGGACGATTTCTTGACCGCCCATCATGAAATGGGTCATATTCAATACGATATGGCCTACGCTATGCAACCATACTTGTTGAGAAACGGTG CTAACGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCTGCTACTCCAAAGCATTTGAAGAACATTGGTTTGTTGCCACCAGATTTTTACGAG GACAACGAAACTGAAATCAACTTCTTGTTGAAGCAGGCCTTGACCATTGTCGGTACTTTGCCATTTACTTACATGCTGGAAAAGTGGCGTTGGATGGTTTTTTC CGGTCAAATTCCAAAGGAGCAGTGGATGAAGAAGTGGTGGGAAATGAAGAGAGAAATTGTCGGTGTTGTTGAGCCAGTTCCACATGATGAAACTTACTGTGA TCCAGCTTCTTTGTTTCACGTTGCTAACGATTACTCCTTTATCCGTTACTACACCCGTACTATTTACCAGTTTCAGTTTCAGGAGGCCTTGTGCCAAACCGCCAA GCATGAAGGTCCATTGCATAAGTGTGATATTTCCAACTCCGCCGAAGCCGGTCAAAAGTTGTTGCAAATGTTGTCTTTGGGTAAGTCCAAGCCATGGACTTTGGCTTTGGAAAGAGTTGTTGGTACTAAGAACATGGACGTTAGACCATTGTTGAACTACTTTGAGCCATTGTTGACTTGGTTGAAGGAACAAAACAAGAACTCCTT TGTCGGTTGGAACACTGATTGGTCTCCATACGCTGCT
SEQ ID NO.19:MfACE2-740(土拨鼠)核苷酸序列
TCTACTATCGAGGAATTGGCCAAGACTTTTTTGGATAAGTTTAACCAGGAGGCCGAGGACTTGGATTACCAGCGTTCTTTGGCTTCTTGGAACTACAACAC TAACATTACCAAGGAGAACACCCAGAAGATGAACGAGGCTGAAGCTAAGTGGTCTGCTTTTTACGAAAAGCAATCTAAGTTGGCGAAGGCCTACCCATTGCA AGAAATTCAAAACTTTACCTTGAAGCGTCAGTTGCAGGCTTTGCAACAATCCGGTTCTTCTGCTTTGTCTGCTAACAAGAGAGAACAATTGAACACCATTTTGA ACACCATGTCCACCATCTACTCTACCGGTAAGGTTTGTAACCCAAAGAAGCCACAAGAATGTTTGTTGTTGGAACCCGGTTTGGATGGTATTATGGCTAACTCT ACTGATTACAACGAGCGTTTGTGGGTTTGGGAAGGTTGGAGATCCAAGGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTCGTTTTGAAGAACGAG ATGGCTAGAGCTAACAACTACGAAGATTACGGTGATTACTGGAGAGGTGATTACGAAGCTGAAGGTGCTGATGGTTACGGTTACAACCATAACCAATTGATTG AGGACGTTGAGAGAACTTTTGCCGAAATTAAGCCATTGTACGAGCATTTGCATGCCTACGTTAGAGCTAAGTTGATGAACACTTACCCATCTTACATTTCCCCC ACTGGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACTCTTTGACCGTCCCATTTCCAGAAAAGCCAAACATTGACGTT ACTGACGCCATGATCAAGCAGAACTGGAACGCTGTTAGAATTTTCAAGGAGGCTGAAAAGTTTTTCGTTTCCGTTGGTTTGCCAAACATGACCCAGGGTTTTTG GGAAAACTCTATGTTGACCGAACCAACTGATGGTAGAAAGGTTGTTTGTCATCCAACTGCTTGGGATTTGCAAAAGGGTGATTTTAGAATTAAGATGTGCACC AAGGTCACCATGGATAACTTCTTGACTGCTCATCATGAAATGGGTCATATTCAGTACAACATGGCCTACGCTATTCAGCCATACTTGTTGAGAAACGGTGCTAA CGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCTACTACCCCAAAGCATTTGAAGTCTATTGGTTTGTTGCCCTCCGATTTTCGTGAGGATAA CGAAACTGAAATTAACTTCTTGTTGAAGCAGGCCTTGACCATCGTTGGTGCTTTGCCATTTACTTACATGTTGGAAAAGTGGCGTTGGATGGTTTTTAAGGGTG AAATTCCAAAGGACCAGTGGATGAAGAAGTGGTGGGAAATGAAGAGAGAAATTGTCGGTGTTATGGAGCCAGTTCCACATGATGAAACTTACTGTGATCCAG CTGCTTTGTACCATGTTTCTAACGATTTTTCCTTTATCCGTTACTACACCAGAACCATTTACCAGTTCCAGTTTCAGGAAGCTTTGTGTCAAGCCGCTAAGCATG AAGGTCCATTGCATAAGTGTGATATTTCCAACTCTACCGAGGCCGGTCAAAAGTTGTTGAACATGTTGAGATTGGGTAAGTCCAAGCCATGGACTTTGGCTTTG GAAAACGTTGTTGGTGCTAGAAACATGGATGTTAGACCATTGTTGAACTACTTCGAGCCCTTGTTTGGTTGGTTGAAGGATCAGAACAGAAACTCTTTTGTCGG TTGGAACACCAACTGGTCTCCATACACTGATCAGTCTATCAAGGTCAGAATCTCTTTGAAGTCCGCTTTGGGTGAGGAAGCTTACCAATGGAACGATAACGAA ATGTACTTGTTCCGTTCTTCCGTTGCCTACGCTATGAGAATGTACTTTTCTAAGGTTAAGAACCAGACCATCCCCTTCGGTGAGGAAGATGTTTGGGTTTCTGAT TTGAAGCCAAGAATTTCCTTTAACTTCTTCGTCACCACCCCACAGAACGCTTCTGATATTATTCCAAGAACTGACGTCGAAAAGGCTATTCGTATGTCCAGAGG TAGAATTAACGGTGTCTTTAGATTGGACGATAACTCCTTGGAATTTCTGGGTATCCAGCCAACCTTGGGTCCACCATACCAACCACCAGTTACT
SEQ ID NO.20:MfACE2-615(土拨鼠)核苷酸序列
TCTACTATCGAGGAATTGGCCAAGACTTTTTTGGATAAGTTTAACCAGGAGGCCGAGGACTTGGATTACCAGCGTTCTTTGGCTTCTTGGAACTACAACAC TAACATTACCAAGGAGAACACCCAGAAGATGAACGAGGCTGAAGCTAAGTGGTCTGCTTTTTACGAAAAGCAATCTAAGTTGGCGAAGGCCTACCCATTGCA AGAAATTCAAAACTTTACCTTGAAGCGTCAGTTGCAGGCTTTGCAACAATCCGGTTCTTCTGCTTTGTCTGCTAACAAGAGAGAACAATTGAACACCATTTTGA ACACCATGTCCACCATCTACTCTACCGGTAAGGTTTGTAACCCAAAGAAGCCACAAGAATGTTTGTTGTTGGAACCCGGTTTGGATGGTATTATGGCTAACTCT ACTGATTACAACGAGCGTTTGTGGGTTTGGGAAGGTTGGAGATCCAAGGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTCGTTTTGAAGAACGAG ATGGCTAGAGCTAACAACTACGAAGATTACGGTGATTACTGGAGAGGTGATTACGAAGCTGAAGGTGCTGATGGTTACGGTTACAACCATAACCAATTGATTG AGGACGTTGAGAGAACTTTTGCCGAAATTAAGCCATTGTACGAGCATTTGCATGCCTACGTTAGAGCTAAGTTGATGAACACTTACCCATCTTACATTTCCCCC ACTGGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACTCTTTGACCGTCCCATTTCCAGAAAAGCCAAACATTGACGTT ACTGACGCCATGATCAAGCAGAACTGGAACGCTGTTAGAATTTTCAAGGAGGCTGAAAAGTTTTTCGTTTCCGTTGGTTTGCCAAACATGACCCAGGGTTTTTG GGAAAACTCTATGTTGACCGAACCAACTGATGGTAGAAAGGTTGTTTGTCATCCAACTGCTTGGGATTTGCAAAAGGGTGATTTTAGAATTAAGATGTGCACC AAGGTCACCATGGATAACTTCTTGACTGCTCATCATGAAATGGGTCATATTCAGTACAACATGGCCTACGCTATTCAGCCATACTTGTTGAGAAACGGTGCTAA CGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCTACTACCCCAAAGCATTTGAAGTCTATTGGTTTGTTGCCCTCCGATTTTCGTGAGGATAA CGAAACTGAAATTAACTTCTTGTTGAAGCAGGCCTTGACCATCGTTGGTGCTTTGCCATTTACTTACATGTTGGAAAAGTGGCGTTGGATGGTTTTTAAGGGTG AAATTCCAAAGGACCAGTGGATGAAGAAGTGGTGGGAAATGAAGAGAGAAATTGTCGGTGTTATGGAGCCAGTTCCACATGATGAAACTTACTGTGATCCAG CTGCTTTGTACCATGTTTCTAACGATTTTTCCTTTATCCGTTACTACACCAGAACCATTTACCAGTTCCAGTTTCAGGAAGCTTTGTGTCAAGCCGCTAAGCATG AAGGTCCATTGCATAAGTGTGATATTTCCAACTCTACCGAGGCCGGTCAAAAGTTGTTGAACATGTTGAGATTGGGTAAGTCCAAGCCATGGACTTTGGCTTTG GAAAACGTTGTTGGTGCTAGAAACATGGATGTTAGACCATTGTTGAACTACTTCGAGCCCTTGTTTGGTTGGTTGAAGGATCAGAACAGAAACTCTTTTGTCGG TTGGAACACCAACTGGTCTCCATACACTGAT
SEQ ID NO.21:PlACE2-740(果子狸)核苷酸序列
TCTACTACTGAAGAGTTGGCCAAGACTTTTTTGGAAACTTTCAACTACGAGGCCCAAGAGTTGTCTTACCAATCTTCTGTTGCTTCTTGGAACTACAACAC TAACATTACCGATGAGAACGCCAAGAACATGAACGAAGCTGGTGCTAAGTGGTCTGCTTACTACGAAGAACAATCTAAGTTGGCCCAAACTTACCCATTGGCT GAAATTCAAGATGCCAAGATTAAGCGTCAGTTGCAGGCTTTGCAACAGTCTGGTTCTTCTGTTTTGTCTGCTGATAAGTCTCAACGTTTGAACACTATTTTGAA CGCCATGTCTACTATCTACTCCACTGGTAAGGCTTGTAACCCAAACAACCCACAAGAATGTTTGTTGTTGGAACCAGGTTTGGATAACATTATGGAGAACTCCA AGGACTACAACGAACGTTTGTGGGCTTGGGAAGGTTGGAGAGCTGAAGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTCGCTTTGAAGAACGAGA TGGCCAGAGCTAACAACTACGAAGATTACGGTGATTACTGGAGAGGTGATTACGAAGAAGAATGGACTGGTGGTTACAACTACTCTAGAAACCAATTGATTC AGGACGTCGAGGACACTTTTGAACAAATTAAGCCATTGTACCAGCACTTGCACGCCTACGTTAGAGCCAAGTTGATGGATACTTACCCATCTAGAATTTCCCG TACCGGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGACTGTCCCATTTGGTCAAAAGCCAAACATTGATG TTACCGACGCTATGGTTAACCAGAACTGGGATGCTAGAAGAATTTTCAAGGAGGCCGAAAAGTTTTTCGTCTCCGTTGGTTTGCCAAACATGACCCAAGGTTTT TGGGAAAACTCTATGTTGACTGAGCCCGGCGATGGTAGAAAGGTTGTTTGTCATCCAACTGCTTGGGATTTGGGTAAGGGTGATTTTAGAATTAAGATGTGCA CCAAGGTTACCATGGACGACTTTTTGACTGCTCATCATGAAATGGGTCATATCCAGTACGATATGGCCTACGCTGCTCAACCATTTTTGTTGAGAAACGGTGCT AACGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCCGCTACTCCAAACCATTTGAAGACTATTGGTTTGTTGTCCCCAGCCTTTTCCGAGGAC AACGAAACTGAGATTAACTTCTTGTTGAAGCAGGCCTTGACCATTGTCGGTACTTTGCCATTTACTTACATGTTGGAAAAGTGGCGTTGGATGGTTTTTAAGGG TGCTATTCCAAAGGAACAGTGGATGCAAAAGTGGTGGGAAATGAAGAGAAACATTGTTGGTGTTGTCGAGCCAGTTCCACATGATGAAACTTACTGTGATCCA GCTTCTTTGTTTCACGTTGCCAACGATTACTCTTTTATCCGTTACTACACCCGTACCATTTACCAATTTCAGTTCCAGGAGGCTTTGTGCCAAATTGCTAAGCAT GAAGGTCCATTGCATAAGTGTGATATTTCCAACTCTACTGAGGCCGGTAAGAAGTTGTTGGAAATGTTGTCTTTGGGCCGTTCTGAACCATGGACTTTGGCTTT GGAAAGAGTTGTTGGTGCTAAGAACATGAACGTTACTCCATTGTTGAACTACTTCGAGCCATTGTTTACCTGGTTGAAGGAACAGAACCGTAACTCTTTTGTTG GTTGGGACACCGATTGGAGACCATACTCTGATCAGTCCATCAAGGTTAGAATCTCTTTGAAGTCCGCTTTGGGTGAGAAGGCTTACGAATGGAACGATAACGA AATGTACTTGTTCCGTTCCTCCATTGCCTACGCTATGCGTGAATACTTTTCTAAGGTTAAGAACCAGACCATCCCCTTCGTTGAGGATAACGTTTGGGTTTCTGA TTTGAAGCCAAGAATTTCCTTCAACTTCTTCGTCACCTTTTCCAACAACGTTTCCGACGTTATTCCACGTTCTGAGGTTGAAGATGCTATTCGCATGTCCCGTTC TAGAATTAACGATGCCTTTAGATTGGACGACAACTCCTTGGAATTTTTGGGTATCGAGCCAACTTTGTCTCCACCATACAGACCACCAGTTACT
SEQ ID NO.22:PlACE2-615(果子狸)核苷酸序列
TCTACTACTGAAGAGTTGGCCAAGACTTTTTTGGAAACTTTCAACTACGAGGCCCAAGAGTTGTCTTACCAATCTTCTGTTGCTTCTTGGAACTACAACAC TAACATTACCGATGAGAACGCCAAGAACATGAACGAAGCTGGTGCTAAGTGGTCTGCTTACTACGAAGAACAATCTAAGTTGGCCCAAACTTACCCATTGGCT GAAATTCAAGATGCCAAGATTAAGCGTCAGTTGCAGGCTTTGCAACAGTCTGGTTCTTCTGTTTTGTCTGCTGATAAGTCTCAACGTTTGAACACTATTTTGAA CGCCATGTCTACTATCTACTCCACTGGTAAGGCTTGTAACCCAAACAACCCACAAGAATGTTTGTTGTTGGAACCAGGTTTGGATAACATTATGGAGAACTCCA AGGACTACAACGAACGTTTGTGGGCTTGGGAAGGTTGGAGAGCTGAAGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTCGCTTTGAAGAACGAGA TGGCCAGAGCTAACAACTACGAAGATTACGGTGATTACTGGAGAGGTGATTACGAAGAAGAATGGACTGGTGGTTACAACTACTCTAGAAACCAATTGATTC AGGACGTCGAGGACACTTTTGAACAAATTAAGCCATTGTACCAGCACTTGCACGCCTACGTTAGAGCCAAGTTGATGGATACTTACCCATCTAGAATTTCCCG TACCGGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGACTGTCCCATTTGGTCAAAAGCCAAACATTGATG TTACCGACGCTATGGTTAACCAGAACTGGGATGCTAGAAGAATTTTCAAGGAGGCCGAAAAGTTTTTCGTCTCCGTTGGTTTGCCAAACATGACCCAAGGTTTT TGGGAAAACTCTATGTTGACTGAGCCCGGCGATGGTAGAAAGGTTGTTTGTCATCCAACTGCTTGGGATTTGGGTAAGGGTGATTTTAGAATTAAGATGTGCA CCAAGGTTACCATGGACGACTTTTTGACTGCTCATCATGAAATGGGTCATATCCAGTACGATATGGCCTACGCTGCTCAACCATTTTTGTTGAGAAACGGTGCT AACGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCCGCTACTCCAAACCATTTGAAGACTATTGGTTTGTTGTCCCCAGCCTTTTCCGAGGAC AACGAAACTGAGATTAACTTCTTGTTGAAGCAGGCCTTGACCATTGTCGGTACTTTGCCATTTACTTACATGTTGGAAAAGTGGCGTTGGATGGTTTTTAAGGG TGCTATTCCAAAGGAACAGTGGATGCAAAAGTGGTGGGAAATGAAGAGAAACATTGTTGGTGTTGTCGAGCCAGTTCCACATGATGAAACTTACTGTGATCCA GCTTCTTTGTTTCACGTTGCCAACGATTACTCTTTTATCCGTTACTACACCCGTACCATTTACCAATTTCAGTTCCAGGAGGCTTTGTGCCAAATTGCTAAGCAT GAAGGTCCATTGCATAAGTGTGATATTTCCAACTCTACTGAGGCCGGTAAGAAGTTGTTGGAAATGTTGTCTTTGGGCCGTTCTGAACCATGGACTTTGGCTTT GGAAAGAGTTGTTGGTGCTAAGAACATGAACGTTACTCCATTGTTGAACTACTTCGAGCCATTGTTTACCTGGTTGAAGGAACAGAACCGTAACTCTTTTGTTG GTTGGGACACCGATTGGAGACCATACTCTGAT
SEQ ID NO.23:PsACE2-740(中华鳖)核苷酸序列
GATATCACCCAAGAGGCCATTAACTTTTTGTCCGAATTTAACGTTCAGGCCGAAGATTTGTCTTACGCTTCTTCTTTGGCTTCTTGGAACTACAACACTAAC ATTACCGATGAGAACGCCAAGAAGATGAACGAGGCTGGTGCTAAGTGGTCTGTTTTTTACGATGAAGCTTCTACCAACGCCTCCAAGTACGCTATTGATAAGA TCACCAACCACACTGTCAAGTTGCAATTGCAATCTTTGCAAGGTAAGGGTACTTCTGTTTTGTCTGGTGAAAAGTACAACGAGTTGAACAAGATTTTGTCCACC ATGTCTACCTTCTACTCTACTGGTACTGTTTGTAAGCCAGATAACCCAGATATTTGCTTGCCATTGGAACCAGGTTTGGATGCTATTATGGCTTCTTCTACTGAT TACTTCGAGCGTTTGTGGGCCTGGGAAGGTTGGAGAGCTGATGTTGGTAAGAAGATGAGAGAATTGTACGAGAGATACGTCGAATTGGAGAACGAGGCCGCT AGATTGAACAAGTACTCTGATTACGGTGATTACTGGAGAGGTAACTACGAAGTTAACGATCCAACTGAATACGCCTACTCTAGAAACCAATTGATGGAGGATG TTGAGGCCACCTTCGAACAGATTAAGCCATTGTACAGAGAGTTGCATGCTTACGTTAGATACAGATTGGAAAAGTTCTACGGTTCCGACCATATCTCCTCCACT GGTTGTTTGCCAGCCCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACGCTTTGACTGTGCCATACCCAGATAAGCCAAACATTGATGTTAC TTCTGAGATGGTCAAGAAGAACTGGAACGCCACTAAGATTTTTAAGGCCGCCGAAGATTTTTTCATGTCCGTTGGTTTGTACAAGATGACCGAAGGTTTTTGGA AGAACTCTATGATTACCGAGCCAAACGATGGTAGAAAGGTTGTTTGTCATCCAACTGCTTGGGATATGGGTAAGAAGGATTACAGAATTAAGATGTGCACCAA GGTCTCTATGGATGACTTTTTGACCGTCCACCATGAAATGGGTCATATTGAATACGATATGGCCTACTCTAACTTGTCCTACTTGTTGCGTTCTGGTGCCAACGA AGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCTGCTACTCCAAAGCATTTGAAGTCTTTGGATTTGTTGGAGCCAACTTTTCAGGAAGATAACG AAACTGACATCAACTTCTTGTTGAAGCAGGCCTTGACTATTGTTGGTACTATGCCATTTACCTACATGTTGGAAAAGTGGAGATGGATGGTTTTTAAGGGTGATATTCCAAAGGACGAGTGGATGAAGAAGTGGTGGGAAATGAAGAGAGCTATTGTTGGTGTTGTTGAGCCAGTTCCACATGATGAAACTTACTGTGATCCAGCTG CTTTGTTTCATGTTGCTAACGATTACTCTTTCATCCGTTACTACACCAGAACCATTTACCAGTTTCAGTTTCAGGAGGCCTTGTGCAAGGCTGCTAACCATGGTG GTTTGTTGCATACTTGTGATATTACCAACTCCATGGCCGCTGGTCAAAAGTTGAGAGATATGTTGGCTTTGGGTAGATCCCAACCATGGACTAAGGCTTTGGAA TCTATTACTGGTGAAAAGAAGATGAACGCCACCCCATTGTTGCATTACTTTGAACCATTGTACCAGTGGTTGATTAAGAACAACTCTGGTAGAGCTGTTGGTTG GAACACTTTTTGGTCTCCATACTCTGGTAACGCTATCAAGGTCAGAATCTCTTTGAAGACCGCTTTGGGTGATAACGCTTACGAATGGGATGAAAACGAATTGT ACTTTTTCAAGTCCTCCATCGCCTACGCTATGAGAAAGTACTTTTTGGAGGTCAAGAACCAGACCGTCTCCTTTCAATGTACTGATATTCATGTCTGGGCCGTTA CCCAACGTGTTTCTTTTTACTTTGCTGTCTCTATGCCAGGTAACGCTACTGATTTTATTCCAAAGTCTGAGGTCGAGACCGCTATCAGAATGTCCAGAGGTAGA ATTAACGAAGCCTTTCGTTTGGACGATAACACCTTGGAATTTGAGGGTTTGTTGCCAACTTTGGCTTCTCCATACGAACCACCAGTTACT
SEQ ID NO.24:PsACE2-615(中华鳖)核苷酸序列
GATATCACCCAAGAGGCCATTAACTTTTTGTCCGAATTTAACGTTCAGGCCGAAGATTTGTCTTACGCTTCTTCTTTGGCTTCTTGGAACTACAACACTAAC ATTACCGATGAGAACGCCAAGAAGATGAACGAGGCTGGTGCTAAGTGGTCTGTTTTTTACGATGAAGCTTCTACCAACGCCTCCAAGTACGCTATTGATAAGA TCACCAACCACACTGTCAAGTTGCAATTGCAATCTTTGCAAGGTAAGGGTACTTCTGTTTTGTCTGGTGAAAAGTACAACGAGTTGAACAAGATTTTGTCCACC ATGTCTACCTTCTACTCTACTGGTACTGTTTGTAAGCCAGATAACCCAGATATTTGCTTGCCATTGGAACCAGGTTTGGATGCTATTATGGCTTCTTCTACTGAT TACTTCGAGCGTTTGTGGGCCTGGGAAGGTTGGAGAGCTGATGTTGGTAAGAAGATGAGAGAATTGTACGAGAGATACGTCGAATTGGAGAACGAGGCCGCT AGATTGAACAAGTACTCTGATTACGGTGATTACTGGAGAGGTAACTACGAAGTTAACGATCCAACTGAATACGCCTACTCTAGAAACCAATTGATGGAGGATG TTGAGGCCACCTTCGAACAGATTAAGCCATTGTACAGAGAGTTGCATGCTTACGTTAGATACAGATTGGAAAAGTTCTACGGTTCCGACCATATCTCCTCCACT GGTTGTTTGCCAGCCCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACGCTTTGACTGTGCCATACCCAGATAAGCCAAACATTGATGTTAC TTCTGAGATGGTCAAGAAGAACTGGAACGCCACTAAGATTTTTAAGGCCGCCGAAGATTTTTTCATGTCCGTTGGTTTGTACAAGATGACCGAAGGTTTTTGGA AGAACTCTATGATTACCGAGCCAAACGATGGTAGAAAGGTTGTTTGTCATCCAACTGCTTGGGATATGGGTAAGAAGGATTACAGAATTAAGATGTGCACCAA GGTCTCTATGGATGACTTTTTGACCGTCCACCATGAAATGGGTCATATTGAATACGATATGGCCTACTCTAACTTGTCCTACTTGTTGCGTTCTGGTGCCAACGA AGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCTGCTACTCCAAAGCATTTGAAGTCTTTGGATTTGTTGGAGCCAACTTTTCAGGAAGATAACG AAACTGACATCAACTTCTTGTTGAAGCAGGCCTTGACTATTGTTGGTACTATGCCATTTACCTACATGTTGGAAAAGTGGAGATGGATGGTTTTTAAGGGTGATATTCCAAAGGACGAGTGGATGAAGAAGTGGTGGGAAATGAAGAGAGCTATTGTTGGTGTTGTTGAGCCAGTTCCACATGATGAAACTTACTGTGATCCAGCTG CTTTGTTTCATGTTGCTAACGATTACTCTTTCATCCGTTACTACACCAGAACCATTTACCAGTTTCAGTTTCAGGAGGCCTTGTGCAAGGCTGCTAACCATGGTG GTTTGTTGCATACTTGTGATATTACCAACTCCATGGCCGCTGGTCAAAAGTTGAGAGATATGTTGGCTTTGGGTAGATCCCAACCATGGACTAAGGCTTTGGAA TCTATTACTGGTGAAAAGAAGATGAACGCCACCCCATTGTTGCATTACTTTGAACCATTGTACCAGTGGTTGATTAAGAACAACTCTGGTAGAGCTGTTGGTTG GAACACTTTTTGGTCTCCATACTCTGGT
SEQ ID NO.25:RnACE2-740(褐家鼠)核苷酸序列
TCTTTGATTGAGGAAAAGGCCGAATCTTTCTTGAACAAGTTCAACCAAGAAGCCGAAGACTTGTCTTACCAGTCTTCTTTGGCTTCCTGGAACTACAACAC TAACATTACTGAAGAGAACGCCCAGAAGATGAACGAGGCTGCTGCTAAGTGGTCTGCTTTTTACGAAGAACAATCTAAGATCGCCCAGAACTTTTCTTTGCAA GAGATTCAGAACGCCACTATCAAGAGACAATTGAAGGCTTTGCAACAGTCTGGTTCTTCTGCTTTGTCTCCAGATAAGAACAAGCAATTGAACACCATCTTGA ACACCATGTCCACCATCTACTCCACTGGTAAGGTTTGTAACTCTATGAACCCACAAGAATGCTTCTTGTTGGAGCCAGGTTTGGATGAAATTATGGCTACTTCT ACTGACTACAACCGTCGTTTGTGGGCTTGGGAAGGTTGGAGAGCTGAAGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTCGTCTTGAAGAACGAG ATGGCCAGAGCCAACAACTACGAAGATTACGGTGATTACTGGAGAGGTGATTACGAAGCTGAAGGTGTTGAAGGTTACAACTACAACAGAAACCAATTGATC GAGGACGTCGAGAACACTTTTAAGGAGATTAAGCCATTGTACGAGCAGTTGCATGCTTACGTTAGAACCAAGTTGATGGAAGTTTACCCCTCTTACATTTCCCC AACTGGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGACTACCCCATTTTTGCAGAAGCCAAACATTGACG TTACTGACGCTATGGTTAACCAATCTTGGGATGCTGAAAGAATTTTCAAGGAGGCCGAGAAGTTCTTCGTCTCTGTTGGTTTGCCACAAATGACTCCAGGTTTT TGGACTAACTCTATGTTGACTGAACCAGGTGATGATAGAAAGGTTGTTTGTCATCCAACTGCCTGGGATTTGGGTCATGGTGATTTTAGAATTAAGATGTGCAC CAAGGTCACCATGGACAACTTTTTGACCGCCCATCATGAAATGGGTCATATTCAATACGATATGGCCTACGCTAAGCAACCATTTTTGTTGAGAAACGGTGCT AACGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCTGCTACTCCAAAGCATTTGAAGTCTATTGGTTTGTTGCCATCCAACTTTCAGGAGGA CAACGAAACTGAGATTAACTTTTTGTTGAAGCAGGCCTTGACCATTGTCGGTACTTTGCCATTTACTTACATGTTGGAGAAGTGGAGATGGATGGTTTTTCAAG ATAAGATCCCACGTGAGCAGTGGACTAAGAAGTGGTGGGAAATGAAGAGAGAAATTGTCGGTGTTGTCGAGCCATTGCCACATGATGAAACTTACTGTGATC CAGCTTCTTTGTTTCACGTCTCTAACGATTACTCTTTCATCCGTTACTACACCCGTACTATTTACCAGTTCCAATTCCAGGAGGCCTTGTGCCAGGCTGCTAAGC ATGATGGTCCATTGCATAAGTGTGATATTTCCAACTCTACCGAGGCCGGTCAAAAGTTGTTGAACATGTTGTCTTTGGGTAACTCCGGTCCATGGACTTTGGCT TTGGAAAACGTTGTTGGTTCTAGAAACATGGACGTTAAGCCATTGTTGAACTACTTCCAGCCATTGTTTGTTTGGTTGAAGGAACAAAACCGCAACTCCACCGT TGGTTGGTCTACTGATTGGTCTCCATACGCTGATCAGTCCATTAAGGTCAGAATTTCCTTGAAGTCTGCCTTGGGTAAGAACGCCTACGAATGGACTGATAACG AAATGTACTTGTTCCGTTCCTCCGTCGCTTACGCTATGAGAGAATACTTTTCCAGAGAAAAGAACCAGACCGTTCCATTCGGTGAGGCTGATGTTTGGGTTTCT GATTTGAAGCCAAGAGTTTCTTTTAACTTCTTCGTCACCTCCCCAAAGAACGTCTCTGATATCATCCCAAGATCCGAAGTTGAAGAAGCCATTAGAATGTCTAG AGGCAGAATTAACGACATCTTCGGTTTGAACGACAACTCCTTGGAATTTTTGGGTATCTACCCAACCTTGAAGCCACCATACGAACCACCAGTTACT
SEQ ID NO.26:RnACE2-615(褐家鼠)核苷酸序列
TCTTTGATTGAGGAAAAGGCCGAATCTTTCTTGAACAAGTTCAACCAAGAAGCCGAAGACTTGTCTTACCAGTCTTCTTTGGCTTCCTGGAACTACAACAC TAACATTACTGAAGAGAACGCCCAGAAGATGAACGAGGCTGCTGCTAAGTGGTCTGCTTTTTACGAAGAACAATCTAAGATCGCCCAGAACTTTTCTTTGCAA GAGATTCAGAACGCCACTATCAAGAGACAATTGAAGGCTTTGCAACAGTCTGGTTCTTCTGCTTTGTCTCCAGATAAGAACAAGCAATTGAACACCATCTTGA ACACCATGTCCACCATCTACTCCACTGGTAAGGTTTGTAACTCTATGAACCCACAAGAATGCTTCTTGTTGGAGCCAGGTTTGGATGAAATTATGGCTACTTCT ACTGACTACAACCGTCGTTTGTGGGCTTGGGAAGGTTGGAGAGCTGAAGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTCGTCTTGAAGAACGAG ATGGCCAGAGCCAACAACTACGAAGATTACGGTGATTACTGGAGAGGTGATTACGAAGCTGAAGGTGTTGAAGGTTACAACTACAACAGAAACCAATTGATC GAGGACGTCGAGAACACTTTTAAGGAGATTAAGCCATTGTACGAGCAGTTGCATGCTTACGTTAGAACCAAGTTGATGGAAGTTTACCCCTCTTACATTTCCCC AACTGGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGACTACCCCATTTTTGCAGAAGCCAAACATTGACG TTACTGACGCTATGGTTAACCAATCTTGGGATGCTGAAAGAATTTTCAAGGAGGCCGAGAAGTTCTTCGTCTCTGTTGGTTTGCCACAAATGACTCCAGGTTTT TGGACTAACTCTATGTTGACTGAACCAGGTGATGATAGAAAGGTTGTTTGTCATCCAACTGCCTGGGATTTGGGTCATGGTGATTTTAGAATTAAGATGTGCAC CAAGGTCACCATGGACAACTTTTTGACCGCCCATCATGAAATGGGTCATATTCAATACGATATGGCCTACGCTAAGCAACCATTTTTGTTGAGAAACGGTGCT AACGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCTGCTACTCCAAAGCATTTGAAGTCTATTGGTTTGTTGCCATCCAACTTTCAGGAGGA CAACGAAACTGAGATTAACTTTTTGTTGAAGCAGGCCTTGACCATTGTCGGTACTTTGCCATTTACTTACATGTTGGAGAAGTGGAGATGGATGGTTTTTCAAG ATAAGATCCCACGTGAGCAGTGGACTAAGAAGTGGTGGGAAATGAAGAGAGAAATTGTCGGTGTTGTCGAGCCATTGCCACATGATGAAACTTACTGTGATC CAGCTTCTTTGTTTCACGTCTCTAACGATTACTCTTTCATCCGTTACTACACCCGTACTATTTACCAGTTCCAATTCCAGGAGGCCTTGTGCCAGGCTGCTAAGC ATGATGGTCCATTGCATAAGTGTGATATTTCCAACTCTACCGAGGCCGGTCAAAAGTTGTTGAACATGTTGTCTTTGGGTAACTCCGGTCCATGGACTTTGGCT TTGGAAAACGTTGTTGGTTCTAGAAACATGGACGTTAAGCCATTGTTGAACTACTTCCAGCCATTGTTTGTTTGGTTGAAGGAACAAAACCGCAACTCCACCGT TGGTTGGTCTACTGATTGGTCTCCATACGCTGAT
SEQ ID NO.27:RfACE2-740(马铁菊头蝠)核苷酸序列
TCTACCACTGAAGATTTGGCCAAGAAGTTTTTGGACGACTTCAACTCCGAGGCTGAAAACTTGTCTCATCAATCTTCTTTGGCCTCCTGGGAATACAACAC TAACATTTCTGACGAGAACGTCCAAAAGATGGATGAAGCCGGTGCTAAGTGGTCTGATTTTTACGAAAAGCAATCCAAGTTGGCCAAGAACTTTTCTTTGGAG GAAATCCACAACGACACCGTTAAGTTGCAGTTGCAAATTTTGCAGCAATCCGGTTCTCCAGTTTTGTCTGAAGATAAGTCTAAGCGTTTGAACTCCATTTTGAA CGCCATGTCTACCATCTACTCCACTGGTAAGGTTTGTAAGCCAAACAACCCACAAGAATGTTTGTTGTTGGAACCAGGTTTGGATAACATTATGGGCACTTCTA AGGACTACAACGAGCGTTTGTGGGCTTGGGAAGGTTGGAGAGCTGAAGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTCGTTTTGAAGAACGAGA TGGCCAGAGGTTACCATTACGAAGATTACGGTGATTACTGGAGAAGAGATTACGAAACTGAAGGTTCTCCAGATTTGGAATACTCTAGAGATCAATTGATCAA GGACGTCGAGCGTATTTTCGCCGAGATCAAGCCATTGTACGAACAATTGCATGCCTACGTTAGAACCAAGTTGATGGATACTTACCCCTTTCACATTTCCCCAA CTGGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGACTGTCCCATTTGGTCAAAAGCCAAACATTGATGTTA CCGACGCCATGTTGAACCAGAACTGGGATGCTAAGAGAATTTTTAAGGAGGCCGAGAAGTTCTTCGTCTCTATTGGTTTGCCAAACATGACCGAAGGTTTTTG GAACAACTCTATGTTGACTGACCCAGGTGATGGTAGAAAGGTTGTTTGTCATCCAACTGCTTGGGATTTGGGTAAGGGTGATTTTAGAATTAAGATGTGCACC AAGGTCACCATGGAGGATTTTTTGACTGCCCATCATGAAATGGGTCATATTCAATACGATATGGCCTACGCTTCTCAACCATACTTGTTGAGAAACGGTGCTAA CGAAGGTTTTCATGAAGCTGTTGGTGAAGTTATGTCTTTGTCTGTTGCTACTCCAAAGCATTTGAAGACTATGGGTTTGTTGTCTTCCGACTTTTTGGAAGATAA CGAGACTGAAATCAACTTCTTGTTCAAGCAGGCCTTGAACATTGTTGGTACCTTGCCATTTACTTACATGTTGGAAAAGTGGCGCTGGATGGTTTTTAAGGGTG AAATTCCAAAGGAGGAGTGGATGAAGAAGTGGTGGGAAATGAAGAGAAAGATTGTCGGTGTTGTTGAGCCAGTTCCACATGATGAAACTTACTGTGATCCAG CTTCTTTGTTTCACGTTGCCAACGATTACTCTTTTATCCGTTACTACACTCGTACCATCTTCGAGTTCCAGTTTCATGAGGCTTTGTGTCGTATTGCTAAGCATGA TGGTCCATTGCATAAGTGTGATATTTCCAACTCCACCGATGCCGGTGAGAAGTTGCATCAAATGTTGTCTGTTGGTAAGTCCCAACCATGGACTTCTGTTTTGA AGGATTTTGTCGGTTCTAAGAACATGGACGTTGGTCCATTGTTGAGATACTTTGAACCATTGTACACCTGGTTGACCGAACAAAACAGAAAGTCCTTTGTCGGT TGGAACACTGATTGGTCTCCATACGCTGATCAGTCCATTAAGGTTCGTATTTCCTTGAAGTCCGCTTTGGGTGAAAAGGCTTACGAATGGAACAACAACGAAA TGTACTTGTTCCGTTCCTCTGTCGCTTACGCCATGAGAGAATACTTTTTGAAGACCAAGAACCAGACCATTTTGTTCGGTGAGGAAGATGTTTGGGTCTCTAAC TTGAAGCCAAGAATTTCCTTTAACTTCTACGTCACTTCCCCACGTAACTTGTCTGACATTATTCCAAAGCCAGAAGTCGAAGGTGCTATTAGAATGTCTAGATC CAGAATCAACGACGCCTTCCGTTTGGATGATAACTCTTTGGAGTTTTTGGGCATCCAGCCAACCTTGGGTCCACCATACCAACCACCAGTTACT
SEQ ID NO.28:RfACE2-615(马铁菊头蝠)核苷酸序列
TCTACCACTGAAGATTTGGCCAAGAAGTTTTTGGACGACTTCAACTCCGAGGCTGAAAACTTGTCTCATCAATCTTCTTTGGCCTCCTGGGAATACAACAC TAACATTTCTGACGAGAACGTCCAAAAGATGGATGAAGCCGGTGCTAAGTGGTCTGATTTTTACGAAAAGCAATCCAAGTTGGCCAAGAACTTTTCTTTGGAG GAAATCCACAACGACACCGTTAAGTTGCAGTTGCAAATTTTGCAGCAATCCGGTTCTCCAGTTTTGTCTGAAGATAAGTCTAAGCGTTTGAACTCCATTTTGAA CGCCATGTCTACCATCTACTCCACTGGTAAGGTTTGTAAGCCAAACAACCCACAAGAATGTTTGTTGTTGGAACCAGGTTTGGATAACATTATGGGCACTTCTA AGGACTACAACGAGCGTTTGTGGGCTTGGGAAGGTTGGAGAGCTGAAGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTCGTTTTGAAGAACGAGA TGGCCAGAGGTTACCATTACGAAGATTACGGTGATTACTGGAGAAGAGATTACGAAACTGAAGGTTCTCCAGATTTGGAATACTCTAGAGATCAATTGATCAA GGACGTCGAGCGTATTTTCGCCGAGATCAAGCCATTGTACGAACAATTGCATGCCTACGTTAGAACCAAGTTGATGGATACTTACCCCTTTCACATTTCCCCAA CTGGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGACTGTCCCATTTGGTCAAAAGCCAAACATTGATGTTA CCGACGCCATGTTGAACCAGAACTGGGATGCTAAGAGAATTTTTAAGGAGGCCGAGAAGTTCTTCGTCTCTATTGGTTTGCCAAACATGACCGAAGGTTTTTG GAACAACTCTATGTTGACTGACCCAGGTGATGGTAGAAAGGTTGTTTGTCATCCAACTGCTTGGGATTTGGGTAAGGGTGATTTTAGAATTAAGATGTGCACC AAGGTCACCATGGAGGATTTTTTGACTGCCCATCATGAAATGGGTCATATTCAATACGATATGGCCTACGCTTCTCAACCATACTTGTTGAGAAACGGTGCTAA CGAAGGTTTTCATGAAGCTGTTGGTGAAGTTATGTCTTTGTCTGTTGCTACTCCAAAGCATTTGAAGACTATGGGTTTGTTGTCTTCCGACTTTTTGGAAGATAA CGAGACTGAAATCAACTTCTTGTTCAAGCAGGCCTTGAACATTGTTGGTACCTTGCCATTTACTTACATGTTGGAAAAGTGGCGCTGGATGGTTTTTAAGGGTG AAATTCCAAAGGAGGAGTGGATGAAGAAGTGGTGGGAAATGAAGAGAAAGATTGTCGGTGTTGTTGAGCCAGTTCCACATGATGAAACTTACTGTGATCCAG CTTCTTTGTTTCACGTTGCCAACGATTACTCTTTTATCCGTTACTACACTCGTACCATCTTCGAGTTCCAGTTTCATGAGGCTTTGTGTCGTATTGCTAAGCATGA TGGTCCATTGCATAAGTGTGATATTTCCAACTCCACCGATGCCGGTGAGAAGTTGCATCAAATGTTGTCTGTTGGTAAGTCCCAACCATGGACTTCTGTTTTGA AGGATTTTGTCGGTTCTAAGAACATGGACGTTGGTCCATTGTTGAGATACTTTGAACCATTGTACACCTGGTTGACCGAACAAAACAGAAAGTCCTTTGTCGGT TGGAACACTGATTGGTCTCCATACGCTGAT
SEQ ID NO.29:sACE2-740(蝾螈)核苷酸序列
GACGTTACTAACGATGCTAGAGTCTTTTTGGACGCTTTTAACGCTCAAGCTGAAGATTTGTCTTACGAGAACTCTTTGGCTTCCTGGGCTTACAACACTAA CATTACTGAAGAGAACGCCATCAAGATGAACGAAGCCGGTGCTAAGTGGACTGCTTTTTACAAGAAGGCTAACAACAACGCCTCTAGATTTCCAGTTGATCAA ATTACCGATCCCGACATTAAGTTGCAGATTTTGTCCTTGGGTGAGAAGGGTTCCTCCGTCTTGCCAGATGATAAGTACAACAGATTGAACAAGGCCTTGTCTGA CATGTCCACCATTTACTCTACTGGTACTGTTTGTGACAACTCCGCTAAGTGTTTGCAGTTGGAACCAGGTTTGGATTTGATTATGGCTGATTCTACTGACTACCA CAAGCGTTTGTGGGCCTGGGAAGGTTGGAGATCCGAAGTTGGTAAGAAGATGAGACCATTGTACGAAACTTACGTCGATTTGAACAACGAAGCCGCCAAGTT GAACGATTACGCTGATTACGGTGATTACTGGAGAGGTAACTACGAAACTCAAGATTCTGGTAAGTACGCCTACTCTAGAAACGATTTGAAGAGAGATGTCGA GCGTACTTTTAAGGAGATCCAGCCATTGTACAGAGAATTGCATGCCTACGTTAGAGATAAGTTGCGTGGTGTTTACGGTGATAAGTACATTTCTAAGAACGGT TGCTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGGCTGTTCCATACCCAAACCAACCATCTATTGATGTTACTTCC GCCATGAACGCTAAGAAGTGGAACGTTGATAAGATGTTTCGTGAGGCCGAGGACTTCTTTGTTTCTGTCGGTTTGTACAAGATGAACGAGAACTTCTGGAACT TCTCTATGTTGACTGAGCCAAACGACGGTAGAAACGTTGTTTGTCATCCAACTGCTTGGGATATGGGTAAGAACGATTTTAGAATTAAGATGTGCACCAAGGT GAACATGGAGGACTTCTTGACCGTCCACCATGAGATGGGTCACATTCAATACGATATGGCTTACGCTAACTTGTCCTTTTTGTTGCGTAACGGTGCTAACGAGG GTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCTGCTACTCCAAAGCATTTGAAGTCTTTGGATTTGTTGCCACCAACTTTTGTGGAGAACGAAGAA ACCAACATCAACTTCTTGTTGCGCCAGGCTTTGACTATTGTCGCCACCATGCCATTTACTTACATGTTGGAAGAATGGAGATGGAAGGTTTTTAACGGTGAAAT TCCACGTGACCAGTGGATGAAGAAGTGGTGGCAAATGAAGAGAGAAATTGTCGGTGTTATGGAGCCAGTTCCACATGATGAAACTTACTGTGATCCAGCTGCT TTGTTTCATGTTGCTAACGATTACTCTTTCATTCGCTACTACACCCGTACTATCTACCAGTTTCAATTTCAGGAGGCCTTGTGCAAGGCCGCTAACCATAACGGT TCTTTGCATACTTGTGATATCACCAACTCCACCTTGGCTGGTCAAAAGTTGAGAACTATGTTGGCTTTGGGTAACTCTAAGCCATGGACTATGGCTTTGGAATC TATTACTGGTGGTAAGACTATGGACGCCCAACCATTGTTGCATTACTTTGACCCATTGTACACTTGGTTGAGAAAGAACAACATTGACAACAACCGTCAGACC TACTGGGATACTGAATGGTCTGCTTACACTGATTACGAGATTAAGGTTCGTATCTCTTTGCACTCCGCTTTCGGTGACAACGCCTACACTTGGGATTCTGGTGA ACAATACTTGTTTAAGTCCACCATCGCCTACGCTATGATTAAGTACTACTCTGAAGTCAAGAGCGAGCAGGTCCCATTTACTGCTGAAAACGTTTTTGTTACCC GTGAGACCTTGAGAATTTCCTTTTACTTCCACGTCACTGACCCACGTAACATTTCCTCTTTTATCCCAAAGATCGACGTCGAAGATGCCGTTAGATTGTCTAGA GGTAGAATTAACTCTGCCTTCAACTTGGACGACAACACTTTGGAATTTGTGGACATCTTGTCCACCTTGTCCCCATCCGTTGAACCACCAGTTACT
SEQ ID NO.30:sACE2-615(蝾螈)核苷酸序列
GACGTTACTAACGATGCTAGAGTCTTTTTGGACGCTTTTAACGCTCAAGCTGAAGATTTGTCTTACGAGAACTCTTTGGCTTCCTGGGCTTACAACACTAA CATTACTGAAGAGAACGCCATCAAGATGAACGAAGCCGGTGCTAAGTGGACTGCTTTTTACAAGAAGGCTAACAACAACGCCTCTAGATTTCCAGTTGATCAA ATTACCGATCCCGACATTAAGTTGCAGATTTTGTCCTTGGGTGAGAAGGGTTCCTCCGTCTTGCCAGATGATAAGTACAACAGATTGAACAAGGCCTTGTCTGA CATGTCCACCATTTACTCTACTGGTACTGTTTGTGACAACTCCGCTAAGTGTTTGCAGTTGGAACCAGGTTTGGATTTGATTATGGCTGATTCTACTGACTACCA CAAGCGTTTGTGGGCCTGGGAAGGTTGGAGATCCGAAGTTGGTAAGAAGATGAGACCATTGTACGAAACTTACGTCGATTTGAACAACGAAGCCGCCAAGTT GAACGATTACGCTGATTACGGTGATTACTGGAGAGGTAACTACGAAACTCAAGATTCTGGTAAGTACGCCTACTCTAGAAACGATTTGAAGAGAGATGTCGA GCGTACTTTTAAGGAGATCCAGCCATTGTACAGAGAATTGCATGCCTACGTTAGAGATAAGTTGCGTGGTGTTTACGGTGATAAGTACATTTCTAAGAACGGT TGCTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGGCTGTTCCATACCCAAACCAACCATCTATTGATGTTACTTCC GCCATGAACGCTAAGAAGTGGAACGTTGATAAGATGTTTCGTGAGGCCGAGGACTTCTTTGTTTCTGTCGGTTTGTACAAGATGAACGAGAACTTCTGGAACT TCTCTATGTTGACTGAGCCAAACGACGGTAGAAACGTTGTTTGTCATCCAACTGCTTGGGATATGGGTAAGAACGATTTTAGAATTAAGATGTGCACCAAGGT GAACATGGAGGACTTCTTGACCGTCCACCATGAGATGGGTCACATTCAATACGATATGGCTTACGCTAACTTGTCCTTTTTGTTGCGTAACGGTGCTAACGAGG GTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCTGCTACTCCAAAGCATTTGAAGTCTTTGGATTTGTTGCCACCAACTTTTGTGGAGAACGAAGAA ACCAACATCAACTTCTTGTTGCGCCAGGCTTTGACTATTGTCGCCACCATGCCATTTACTTACATGTTGGAAGAATGGAGATGGAAGGTTTTTAACGGTGAAAT TCCACGTGACCAGTGGATGAAGAAGTGGTGGCAAATGAAGAGAGAAATTGTCGGTGTTATGGAGCCAGTTCCACATGATGAAACTTACTGTGATCCAGCTGCT TTGTTTCATGTTGCTAACGATTACTCTTTCATTCGCTACTACACCCGTACTATCTACCAGTTTCAATTTCAGGAGGCCTTGTGCAAGGCCGCTAACCATAACGGT TCTTTGCATACTTGTGATATCACCAACTCCACCTTGGCTGGTCAAAAGTTGAGAACTATGTTGGCTTTGGGTAACTCTAAGCCATGGACTATGGCTTTGGAATC TATTACTGGTGGTAAGACTATGGACGCCCAACCATTGTTGCATTACTTTGACCCATTGTACACTTGGTTGAGAAAGAACAACATTGACAACAACCGTCAGACC TACTGGGATACTGAATGGTCTGCTTACACTGAT
SEQ ID NO.31:SsACE2-740(野猪)核苷酸序列
TCTACTACCGAGGAATTGGCTAAGACTTTTTTGGAAAAGTTCAACTTGGAGGCCGAGGATTTGGCTTACCAATCTTCTTTGGCTTCTTGGAACTACAACAC TAACATTACCGATGAGAACATCCAGAAGATGAACGACGCTAGAGCCAAGTGGTCTGCTTTTTACGAAGAACAATCTCGTATTGCCAAGACCTACCCATTGGAT GAAATTCAAACTTTGATCTTGAAGCGTCAGTTGCAGGCTTTGCAACAGTCCGGTACTTCTGGTTTGTCTGCTGATAAGTCTAAGAGATTGAACACCATCTTGAA CACCATGTCCACTATTTACTCTTCCGGTAAGGTTCTTGATCCAAACAACCCACAAGAATGTTTGGTTTTGGAACCAGGTTTGGATGAAATTATGGAGAACTCTA AGGACTACTCCCGTAGATTGTGGGCTTGGGAATCTTGGAGAGCTGAAGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTCGTCTTGGAGAACGAGAT GGCTAGAGCTAACAACTACGAAGATTACGGTGATTACTGGAGAGGTGATTACGAAGTTACTGGTACTGGTGATTACGATTACTCTAGAAACCAATTGATGGAG GACGTTGAGAGAACTTTCGCTGAAATTAAGCCATTGTACGAACACTTGCACGCCTACGTTAGAGCTAAGTTGATGGATGCTTACCCATCTAGAATTTCCCCAAC TGGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGACTGTCCCATTTGGTGAAAAGCCATCTATTGATGTTAC CGAGGCCATGGTTAACCAGTCTTGGGATGCTATTAGAATCTTTGAGGAAGCGGAGAAGTTTTTCGTCTCTATTGGTTTGCCAAACATGACCCAAGGTTTTTGGA ACAACTCTATGTTGACTGAGCCAGGTGATGGTAGAAAGGTTGTTTGTCATCCAACTGCTTGGGATTTGGGTAAGGGTGATTTTAGAATTAAGATGTGCACCAA GGTCACCATGGATGATTTTTTGACTGCTCATCATGAGATGGGTCACATTCAATACGATATGGCCTACGCTATTCAGCCATACTTGTTGAGAAACGGTGCTAACG AAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCCGCTGCTACTCCACATTACTTGAAGGCTTTGGGTTTGTTGCCACCAGATTTTTACGAAGATTCTG AGACTGAAATCAACTTCTTGTTGAAGCAGGCCTTGACTATTGTCGGTACTTTGCCATTTACTTACATGTTGGAAAAGTGGCGTTGGATGGTTTTTAAGGGTGAAATTCCAAAGGAGCAGTGGATGCAAAAGTGGTGGGAAATGAAGAGAGAAATTGTCGGTGTTGTCGAGCCATTGCCACATGATGAAACTTACTGTGATCCAGCT TGTTTGTTTCACGTCGCTGAAGATTACTCTTTCATCCGTTACTACACCCGTACTATTTACCAGTTTCAGTTCCATGAGGCTTTGTGTAGAACTGCCAAGCATGAA GGTCCATTGTACAAGTGTGATATTTCCAACTCTACCGAGGCTGGTCAAAAGTTGTTGCAAATGTTGTCTTTGGGTAAGTCCGAACCATGGACTTTGGCTTTGGA AAACATTGTTGGTGTTAAGACCATGGACGTCAAGCCATTGTTGTCTTACTTTGAGCCATTGTTGACCTGGTTGAAGGCCCAAAACGGTAACTCTTCTGTTGGTT GGAACACTGATTGGACTCCATACGCTGATCAATCCATCAAGGTTAGAATCTCCTTGAAGTCCGCTTTGGGTAAGGAAGCCTACGAATGGAACGATAACGAAAT GTACTTGTTCCGCTCCTCCATCGCCTACGCTATGCGTAACTACTTTTCTTCTGCTAAGAACGAGACCATCCCATTTGGTGCTGAAGATGTTTGGGTTTCTGATTT GAAGCCAAGAATTTCCTTTAACTTCTTCGTCACCTCCCCAGCCAACATGTCCGATATTATTCCAAGATCCGATGTCGAGAAGGCCATTTCTATGTCTCGTTCTAG AATTAACGACGCCTTCCGTTTGGATGACAACACTTTGGAATTTTTGGGTATCCAGCCAACTTTGGGTCCACCAGATGAACCACCAGTTACT
SEQ ID NO.32:SsACE2-615(野猪)核苷酸序列
TCTACTACCGAGGAATTGGCTAAGACTTTTTTGGAAAAGTTCAACTTGGAGGCCGAGGATTTGGCTTACCAATCTTCTTTGGCTTCTTGGAACTACAACAC TAACATTACCGATGAGAACATCCAGAAGATGAACGACGCTAGAGCCAAGTGGTCTGCTTTTTACGAAGAACAATCTCGTATTGCCAAGACCTACCCATTGGAT GAAATTCAAACTTTGATCTTGAAGCGTCAGTTGCAGGCTTTGCAACAGTCCGGTACTTCTGGTTTGTCTGCTGATAAGTCTAAGAGATTGAACACCATCTTGAA CACCATGTCCACTATTTACTCTTCCGGTAAGGTTCTTGATCCAAACAACCCACAAGAATGTTTGGTTTTGGAACCAGGTTTGGATGAAATTATGGAGAACTCTA AGGACTACTCCCGTAGATTGTGGGCTTGGGAATCTTGGAGAGCTGAAGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTCGTCTTGGAGAACGAGAT GGCTAGAGCTAACAACTACGAAGATTACGGTGATTACTGGAGAGGTGATTACGAAGTTACTGGTACTGGTGATTACGATTACTCTAGAAACCAATTGATGGAG GACGTTGAGAGAACTTTCGCTGAAATTAAGCCATTGTACGAACACTTGCACGCCTACGTTAGAGCTAAGTTGATGGATGCTTACCCATCTAGAATTTCCCCAAC TGGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGACTGTCCCATTTGGTGAAAAGCCATCTATTGATGTTAC CGAGGCCATGGTTAACCAGTCTTGGGATGCTATTAGAATCTTTGAGGAAGCGGAGAAGTTTTTCGTCTCTATTGGTTTGCCAAACATGACCCAAGGTTTTTGGA ACAACTCTATGTTGACTGAGCCAGGTGATGGTAGAAAGGTTGTTTGTCATCCAACTGCTTGGGATTTGGGTAAGGGTGATTTTAGAATTAAGATGTGCACCAA GGTCACCATGGATGATTTTTTGACTGCTCATCATGAGATGGGTCACATTCAATACGATATGGCCTACGCTATTCAGCCATACTTGTTGAGAAACGGTGCTAACG AAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCCGCTGCTACTCCACATTACTTGAAGGCTTTGGGTTTGTTGCCACCAGATTTTTACGAAGATTCTG AGACTGAAATCAACTTCTTGTTGAAGCAGGCCTTGACTATTGTCGGTACTTTGCCATTTACTTACATGTTGGAAAAGTGGCGTTGGATGGTTTTTAAGGGTGAAATTCCAAAGGAGCAGTGGATGCAAAAGTGGTGGGAAATGAAGAGAGAAATTGTCGGTGTTGTCGAGCCATTGCCACATGATGAAACTTACTGTGATCCAGCT TGTTTGTTTCACGTCGCTGAAGATTACTCTTTCATCCGTTACTACACCCGTACTATTTACCAGTTTCAGTTCCATGAGGCTTTGTGTAGAACTGCCAAGCATGAA GGTCCATTGTACAAGTGTGATATTTCCAACTCTACCGAGGCTGGTCAAAAGTTGTTGCAAATGTTGTCTTTGGGTAAGTCCGAACCATGGACTTTGGCTTTGGA AAACATTGTTGGTGTTAAGACCATGGACGTCAAGCCATTGTTGTCTTACTTTGAGCCATTGTTGACCTGGTTGAAGGCCCAAAACGGTAACTCTTCTGTTGGTT GGAACACTGATTGGACTCCATACGCTGAT
SEQ ID NO.33:TeACE2-740(蛇)核苷酸序列
GATGTTACTCAACAAGCCGCTGAATTTTTGAAGCAATTTGACGCCAGAGCCGACGATTTGTACTACGCTGCTTCTATTGCTTCTTGGAACTACAACACTAA CTTGACCGAAGAAAACGCCAAGATTATGCACGAAAAGGACAACATTTTCTCCAAGTTTTACGAGGAGGCCTCTAAGAACGCCTCTATGTACAACGTTAACCAA ATTACCAACGAGACCATTCGTTTGCAGTTGCACTTGTTGCAGAACGTCCCAACTAACTCCTCTACTAAGGATCAATTGGATACCGTTTTGCGTAAGATGTCTAC TATGTACTCCACTGGTACCGTTTGTAAGCAAGATGATCCATTTAACTGCTTGCCCTTGGAGCCAGGTTTGGATGATATTATGGAAAACAACTGGTCCTACTCCG AACGTTTGTGGGCTTGGGAAGGTTGGAGAGCTGATGTTGGTAAGAAGATGAGACCATTGTACGAATCTTACGTCGAGTTGAAGAACAAGTACGCTAGATTGA GAGGTTACGCTGATTACGGTGATTACTGGAGAGCTAACTACGAAGTTGATTTGCCAAAGGAATACCAGTACCAGAGAGCCCAATTGATCACTGACGTCGAAA ACACTTTGCAACAGATTATGCCATTGTACAAGCACTTGCACGCTTACGTTAGAAGACATTTGTACAAGCATTACGGTCCCGAATTTATCAACTTGGAGGGTGCC ATTCCCGCCCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGATGGTCCCATTTCCAAACAAGACTTCTATTGACGTCACCTCCGCT ATGGTCACCAAGAAGTGGACTGTTAACTCTATTTTCAAGGCCGCTGAGCAATTTTTCACCTCCATTGGTTTGTTTCCAATGACCGATAACTTTTGGAACAACTC CATGTTGGAAGAGCCAAAGGATGGTAGAAAGGTTGTTTGTCATCCAACTGCTTGGGATATGGGTAAGAAGGATTACAGAATTAAGATGTGCACCAAGATCAA CATGGAGGACTTCTTGACCGCTCACCATGAAATGGGTCATATTGAATACGACATGGCCTACTCTGATCAGCCATTTTTGTTGAGAAACGGTGCTAACGAAGGT TTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCCGCTACTCCAAAGTACTTGAAGTCTTTGGGTTTGTTGGAACACACCTTTCAAGAGGATACTGAAAC CGATATCAACTTTTTGTTGAAGCAGGCCTTGACCATTGTCGGTACTATGCCATTTACTTACATGTTGGAAAAGTGGCGTTGGATGGTTTTTGCTGAACAAATTC CAAAGGATCAGTGGATGAAGAAGTGGTGGGAAATGAAGAGAGAAATTGTCGGTGTTGTTGAGCCATTGCCACATAACGAAGAATACTGTGATCCAGCTGCTT TGTTTCATGTTGCTAACGATTACTCTTTCATCCGTTACTACACCAGAACCATCTACCAGTTTCAGTTCCAGGAGGCTTTGTGTCAAGCTGCCGGTCATACTGGTG AATTGTACAAGTGTGAAATTTCCCACTCCACCGACGCCGGTCATATTTTGAAGGATATGTTGGCTTTGGGTTCCTCTCAACCATGGACTAAGGCTTTGGAATCT ATTACTAAGTCCCAGAAGATGGACGCCACCCCATTTAGACATTACTTTGACCCATTGTTGAAGTGGTTGGAAAAGCAAAACTCTAACGAGAACGTCGGCTGGA ACGTTAACTGGACTCCATACTCTAAGTACGCCATCAAGGTTAGAATCTCTTTGAAGAGAGCTTTGGGCGATGATGCTTACAACTGGACTGCTTCTGAAATGTAC TTGTTTAAGTCCACCATCGCCTACGCCATGCAAAAGTACTTCTTGGAGATTAAGAACAAGACCGTCTTGTTCCAGACCGACAACGTTCATGTCTCTCCAGTTAC TGAGAGAATTTCTTTTTACTTCACCGTCTCCATGCCAACCAACATCTCTGAATTGGTTCCAAAGTCTGAAGTCGAGGAAGCCATTTCTTTGTCTAGAGATAGAA TTAACGAGGCCTTTCGTTTGACCGACCAGACTTTGGAGTTTGTTGGTTTGTTGCCAACTTTGGCTCCACCATACGAATCTCCAATTACT
SEQ ID NO.34:TeACE2-615(蛇)核苷酸序列
GATGTTACTCAACAAGCCGCTGAATTTTTGAAGCAATTTGACGCCAGAGCCGACGATTTGTACTACGCTGCTTCTATTGCTTCTTGGAACTACAACACTAA CTTGACCGAAGAAAACGCCAAGATTATGCACGAAAAGGACAACATTTTCTCCAAGTTTTACGAGGAGGCCTCTAAGAACGCCTCTATGTACAACGTTAACCAA ATTACCAACGAGACCATTCGTTTGCAGTTGCACTTGTTGCAGAACGTCCCAACTAACTCCTCTACTAAGGATCAATTGGATACCGTTTTGCGTAAGATGTCTAC TATGTACTCCACTGGTACCGTTTGTAAGCAAGATGATCCATTTAACTGCTTGCCCTTGGAGCCAGGTTTGGATGATATTATGGAAAACAACTGGTCCTACTCCG AACGTTTGTGGGCTTGGGAAGGTTGGAGAGCTGATGTTGGTAAGAAGATGAGACCATTGTACGAATCTTACGTCGAGTTGAAGAACAAGTACGCTAGATTGA GAGGTTACGCTGATTACGGTGATTACTGGAGAGCTAACTACGAAGTTGATTTGCCAAAGGAATACCAGTACCAGAGAGCCCAATTGATCACTGACGTCGAAA ACACTTTGCAACAGATTATGCCATTGTACAAGCACTTGCACGCTTACGTTAGAAGACATTTGTACAAGCATTACGGTCCCGAATTTATCAACTTGGAGGGTGCC ATTCCCGCCCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGATGGTCCCATTTCCAAACAAGACTTCTATTGACGTCACCTCCGCT ATGGTCACCAAGAAGTGGACTGTTAACTCTATTTTCAAGGCCGCTGAGCAATTTTTCACCTCCATTGGTTTGTTTCCAATGACCGATAACTTTTGGAACAACTC CATGTTGGAAGAGCCAAAGGATGGTAGAAAGGTTGTTTGTCATCCAACTGCTTGGGATATGGGTAAGAAGGATTACAGAATTAAGATGTGCACCAAGATCAA CATGGAGGACTTCTTGACCGCTCACCATGAAATGGGTCATATTGAATACGACATGGCCTACTCTGATCAGCCATTTTTGTTGAGAAACGGTGCTAACGAAGGT TTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCCGCTACTCCAAAGTACTTGAAGTCTTTGGGTTTGTTGGAACACACCTTTCAAGAGGATACTGAAAC CGATATCAACTTTTTGTTGAAGCAGGCCTTGACCATTGTCGGTACTATGCCATTTACTTACATGTTGGAAAAGTGGCGTTGGATGGTTTTTGCTGAACAAATTC CAAAGGATCAGTGGATGAAGAAGTGGTGGGAAATGAAGAGAGAAATTGTCGGTGTTGTTGAGCCATTGCCACATAACGAAGAATACTGTGATCCAGCTGCTT TGTTTCATGTTGCTAACGATTACTCTTTCATCCGTTACTACACCAGAACCATCTACCAGTTTCAGTTCCAGGAGGCTTTGTGTCAAGCTGCCGGTCATACTGGTG AATTGTACAAGTGTGAAATTTCCCACTCCACCGACGCCGGTCATATTTTGAAGGATATGTTGGCTTTGGGTTCCTCTCAACCATGGACTAAGGCTTTGGAATCT ATTACTAAGTCCCAGAAGATGGACGCCACCCCATTTAGACATTACTTTGACCCATTGTTGAAGTGGTTGGAAAAGCAAAACTCTAACGAGAACGTCGGCTGGA ACGTTAACTGGACTCCATACTCTAAGCAT
SEQ ID NO.35:CsACE2-740(银鲑鱼)核苷酸序列
TCCGATTTGGAAAGACGTGCTCAAGAATTTTTGAACCAGTTCGATGGTAACGCTACCCATTTGATGTACCAATACTCTTTGGCTTCTTGGGCTTACAACAC TGATATTTCTCAAGAGAACTTGGACAAGTTGGGTGTCCAATCTGCTATTTGGGGTGAATACTACTCTACTGTTTCTAAGGAATCCGAGAAGTTCCCAATCGACC AAATCAGAGATCCATTGATTAAGTTGCAGTTGATCTCCTTGCAAGACAAGGGTTCTGGTGCTTTGTCTGCTGATAAGGCTGCTCATTTGAACAAGGTTATGAAC GAAATGTCCTCCATTTACTCCACCGGTACTGTTTGTAAGCGTGAAGATCCATTTGATTGTCAGACTTTGGAACCAGGTTTGGAATCTGTTATGGCTAACATGGA TTCTGACTACTACGAGAGATTGCACGTCTGGGAAGGTTGGAGAGTTGAAGTTGGTAAGAAGATGAGACCATTGTACGAAGATTACGTCGATTTGAAGAACGA GGCTGCTAAGTTGAACGATTACGAAGATTACGGTGATTACTGGAGATCCAACTACGAAACTACTGACGATTCTCCCTACAACTACGCTAGAGGTCAATTGATG ACTGATGTTAGAAGAATCTACAAGGAGATCCTGCCCTTGTACAAGGAGTTGCACGCCTACGTTAGATCCAAGTTGCAGGCTAAGCATCCAGAACATATTCACC CAGAAGGTGGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTGGTTTGTACCCAATTTCTACCCCATTTCCAGAAAAGATCGATATCGAC GTTACTAACGCTATGATCGCCCAAAAGTGGCCAAAGGATAGATTGTTTCAAGAGGCTGAAAAGTTCTTCATGTCCGTCGGTTTGTACAAGATGTTTGACAACTT TTGGAAGGACTCCATGTTGGAAAAGCCAACTGATGGTAGAAAGGTTGTTTGTCATCCAACTGCTTGGGATATGGGTAACAGAGAAGATTTTAGAATCAAGATG TGCACCGAGGTTAACATGGATCATTTTTTGACCGCTCATCACGAGATGGGTCATAACCAATACCAAATGGCTTACAGAAACTTGTCCTACTTGTTGCGTGACGG TGCTAACGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCCGCTGCTACTCCAAAGCATTTGAAGGCTTTGGGTTTGTTGCCAGATGATTTTGTTGA AGACAAGGAGACCGAAATCAACTTCTTGATGAAGCAGGCCTTGACCATTGTTGCTACTTTGCCATTTACTTACATGTTGGAGGAATGGAGATGGCAAGTTTTTT TGGGTACTATTCCAAAGGACCAGTGGATGCAAAGATGGTGGGAAATGAAGAGAGATATGGTTGGTGTTGTTGAGCCATTGCCAAGAGATGAAACTTACTGTG ATCCACCAGCTTTGTTTCATGTTTCTGGTGATTACTCTTTCATCCGTTACTTTACCCGTACCATTTACCAGTTTCAGTTCCAGAAGGCTTTGTGCGAAGCTGCTGG TCATTCTGGTCCATTGTTTAAGTGTGATATTACCAACTCCACCGCCGCCGGTGATAAGTTGAGAACTATGTTGGAATTTGGTCGTTCCAAGTCCTGGACTAGAG CTTTGGAAACTATTTCCGGTAACCCAAAGATGGATTCTGCTCCATTGTTGGATTACTTTAAGGACTTGCACGTCTGGTTGTTGGAAGAGAACAGAAAGAACAA CAGAAAGCCAGGTTGGAAGGCTGCTGAAGATCCATTTTCTGAAAACGCCTACAAGGTTAGATTGTCTTTGAAGGCTGCTATGGGTGATAAGGCTTACAAGTGG AACGCTAACGAAATGTACTTGTTTAAGGCCAACATGGCCTACGCCATGAGACAATACTACTTGGAAGTTAACAAGACCGCCGCTTTGTTTACTACTGAGAACA TTCACACTTACAAGGAGACCGCTAGAATCTCTTTTTACTTCGTTGTCACTGACCCAGCTAACTCCGCTGTTGTTATTCCAAAGGCTGAAGTTGAAGCTGCTATTA GAATGTCTAGAGGTAGAATTAACGACGCCTTTAAGTTGGATGACAAGACTTTGGAGTTCGAAGGTTTGTTGGCTACTTTGGCTCCACCAGTTGAACAACCAGTTACT
SEQ ID NO.36:CsACE2-615(银鲑鱼)核苷酸序列
TCCGATTTGGAAAGACGTGCTCAAGAATTTTTGAACCAGTTCGATGGTAACGCTACCCATTTGATGTACCAATACTCTTTGGCTTCTTGGGCTTACAACAC TGATATTTCTCAAGAGAACTTGGACAAGTTGGGTGTCCAATCTGCTATTTGGGGTGAATACTACTCTACTGTTTCTAAGGAATCCGAGAAGTTCCCAATCGACC AAATCAGAGATCCATTGATTAAGTTGCAGTTGATCTCCTTGCAAGACAAGGGTTCTGGTGCTTTGTCTGCTGATAAGGCTGCTCATTTGAACAAGGTTATGAAC GAAATGTCCTCCATTTACTCCACCGGTACTGTTTGTAAGCGTGAAGATCCATTTGATTGTCAGACTTTGGAACCAGGTTTGGAATCTGTTATGGCTAACATGGA TTCTGACTACTACGAGAGATTGCACGTCTGGGAAGGTTGGAGAGTTGAAGTTGGTAAGAAGATGAGACCATTGTACGAAGATTACGTCGATTTGAAGAACGA GGCTGCTAAGTTGAACGATTACGAAGATTACGGTGATTACTGGAGATCCAACTACGAAACTACTGACGATTCTCCCTACAACTACGCTAGAGGTCAATTGATG ACTGATGTTAGAAGAATCTACAAGGAGATCCTGCCCTTGTACAAGGAGTTGCACGCCTACGTTAGATCCAAGTTGCAGGCTAAGCATCCAGAACATATTCACC CAGAAGGTGGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTGGTTTGTACCCAATTTCTACCCCATTTCCAGAAAAGATCGATATCGAC GTTACTAACGCTATGATCGCCCAAAAGTGGCCAAAGGATAGATTGTTTCAAGAGGCTGAAAAGTTCTTCATGTCCGTCGGTTTGTACAAGATGTTTGACAACTT TTGGAAGGACTCCATGTTGGAAAAGCCAACTGATGGTAGAAAGGTTGTTTGTCATCCAACTGCTTGGGATATGGGTAACAGAGAAGATTTTAGAATCAAGATG TGCACCGAGGTTAACATGGATCATTTTTTGACCGCTCATCACGAGATGGGTCATAACCAATACCAAATGGCTTACAGAAACTTGTCCTACTTGTTGCGTGACGG TGCTAACGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCCGCTGCTACTCCAAAGCATTTGAAGGCTTTGGGTTTGTTGCCAGATGATTTTGTTGA AGACAAGGAGACCGAAATCAACTTCTTGATGAAGCAGGCCTTGACCATTGTTGCTACTTTGCCATTTACTTACATGTTGGAGGAATGGAGATGGCAAGTTTTTT TGGGTACTATTCCAAAGGACCAGTGGATGCAAAGATGGTGGGAAATGAAGAGAGATATGGTTGGTGTTGTTGAGCCATTGCCAAGAGATGAAACTTACTGTG ATCCACCAGCTTTGTTTCATGTTTCTGGTGATTACTCTTTCATCCGTTACTTTACCCGTACCATTTACCAGTTTCAGTTCCAGAAGGCTTTGTGCGAAGCTGCTGG TCATTCTGGTCCATTGTTTAAGTGTGATATTACCAACTCCACCGCCGCCGGTGATAAGTTGAGAACTATGTTGGAATTTGGTCGTTCCAAGTCCTGGACTAGAG CTTTGGAAACTATTTCCGGTAACCCAAAGATGGATTCTGCTCCATTGTTGGATTACTTTAAGGACTTGCACGTCTGGTTGTTGGAAGAGAACAGAAAGAACAA CAGAAAGCCAGGTTGGAAGGCTGCTGAAGATCCATTTTCTGAA
SEQ ID NO.37:RtACE2-740(虹鳟鱼)核苷酸序列
TCCGATTTGGAACGTAGAGCCCAAGAATTTTTGGACCAATTTGACGGTAACGCCACTCATTTGATGTACCAATACTCTTTGGCTTCCTGGGCTTACAACAC TGATATTTCTCAAGAGAACTTGGACAAGTTGGGTGTTCAATCTACTATCTGGGGTGAATACTACTCCACTGTCTCTAAGGAATCTGAAAAGTTTCCAATCGACC AGATATCCGACCCATTGATCAGATTGCAATTGATTTCCTTGCAGGACAAGGGTTCTGGTGCTTTGTCTGCTGATAAGGCTGCTCATTTGAACAAGGTTATGAAC GAAATGTCCTCCATTTACTCCACCGGTACCGTCTGTAAGAGAGAAGATCCATTGGATTGTCAAACCTTGGAGCCAGGTTTGGAATCTGTTATGGCTAACATGG ATTCTGACTACTACGAAAGATTGCACGTCTGGGAAGGTTGGAGAGTTGAAGTTGGTAAGAAGATGAGACCATTGTACGAAGATTACGTCGATTTGAAGAACG AGGCCGCTAAGTTGAACGATTACGAAGATTACGGTGATTACTGGAGATCCAACTACGAAACTATTGACGACTCTCCATACAACTACGCTAGAGGTCAATTGAT GACTGATGTTAGAAGAATCTACAAGGAGATCCTTCCATTGTACAAGGAATTGCACGCCTACGTTCGTTCTAAGTTGCAAGCTAAGCATCCAGAACATATTCAC CCAGAAGGTGGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTGGTTTGTACCCAATTTCTACCCCATTTCCAGAAAAGACCGATATCGA TGTTACTGAGGCTATGATTGCCCAAAAGTGGCCAAAGGATAGATTGTTTCAAGAGGCCGAAAAGTTCTTCATGTCTGTTGGTTTGTACAAGATGTTTGACAACT TCTGGAAGGACTCTATGTTGGAGAAGCCAACCGATGGTAGAAAGGTTGTTTGTCATCCAACTGCTTGGGATATGGGTAACAGAGAAGATTTTAGAATCAAGAT GTGCACGGAGGTCAACATGGACCATTTTTTGACCGCTCACCATGAAATGGGTCATAACCAATACCAAATGGCCTACAGAAACTTGTCTTACTTGTTGCGTGAT GGTGCCAACGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCTGCTACTCCAAAGCATTTGAAGGCTTTGGGTTTGTTGCCAGGTGATTTTGTT GAAGATAAGGAGACCGAAATCAACTTCTTGATGAAGCAGGCTTTGACCATTGTTGCTACTTTGCCATTTACTTACATGTTGGAGGAATGGCGTTGGCAAGTTTT TTTGGGTACTATTCCAAAGGACCAGTGGATGCAAAGATGGTGGGAAATGAAGAGAGATATGGTTGGTGTTGTTGAGCCATTGCCAAGAGATGAAACTTACTGT GATCCACCAGCTTTGTTTCATGTTTCTGGTGATTACTCTTTCATCCGTTACTTTACCAGAACCGTCTACCAATTTCAATTCCAGAAGGCTTTGTGCGAAGCCGCT GGTCATTCTGGTCCATTGTTTAAGTGTGATATTACCAACTCCACCGCCGCTGGTGATAAGTTGAGAACTATGTTGGAATTTGGTCGTTCCAAGTCCTGGACTCG TGCTTTGGAAACTATTTCTGGTAACGCTAAGATGGACTCTGCCCCATTGTTGGATTACTTTAAGGACTTGCATGTCTGGTTGATCGAAGAGAACAGAAAGAAC AACAGAAAGCCAGGTTGGAGAGCTGCTGAAGATCCATTTTCTGCTAACGCTTACAAGGTTAGATTGTCCTTGAAGGCTGCTATGGGTGATAAGGCTTACATGT GGAACGCTAACGAAATGTACTTGTTTAAGGCCAACATGGCCTACGCTATGAGACAATACTACTTGGAAGTTAACAAGACCGCCGCCTTGTTTACCACTGAAAA CATTCATACCTACAAGGAGACTGCCAGAATTTCTTTTTACTTCGTCGTCACCGACCCAGCCAACTCTGCTGTTGTTATTCCAAAGGCTGAAGTTGAAGCTGCTA TTAGAATGTCTAGAGGTAGAATTAACGACGCCTTTAAGTTGGATGATAAGACCTTGGAATTTGAGGGTTTGTTGGCCACTTTGGCCCCACCAGTTGAACAACCAGTTACT
SEQ ID NO.38:RtACE2-615(虹鳟鱼)核苷酸序列
TCCGATTTGGAACGTAGAGCCCAAGAATTTTTGGACCAATTTGACGGTAACGCCACTCATTTGATGTACCAATACTCTTTGGCTTCCTGGGCTTACAACAC TGATATTTCTCAAGAGAACTTGGACAAGTTGGGTGTTCAATCTACTATCTGGGGTGAATACTACTCCACTGTCTCTAAGGAATCTGAAAAGTTTCCAATCGACC AGATATCCGACCCATTGATCAGATTGCAATTGATTTCCTTGCAGGACAAGGGTTCTGGTGCTTTGTCTGCTGATAAGGCTGCTCATTTGAACAAGGTTATGAAC GAAATGTCCTCCATTTACTCCACCGGTACCGTCTGTAAGAGAGAAGATCCATTGGATTGTCAAACCTTGGAGCCAGGTTTGGAATCTGTTATGGCTAACATGG ATTCTGACTACTACGAAAGATTGCACGTCTGGGAAGGTTGGAGAGTTGAAGTTGGTAAGAAGATGAGACCATTGTACGAAGATTACGTCGATTTGAAGAACG AGGCCGCTAAGTTGAACGATTACGAAGATTACGGTGATTACTGGAGATCCAACTACGAAACTATTGACGACTCTCCATACAACTACGCTAGAGGTCAATTGAT GACTGATGTTAGAAGAATCTACAAGGAGATCCTTCCATTGTACAAGGAATTGCACGCCTACGTTCGTTCTAAGTTGCAAGCTAAGCATCCAGAACATATTCAC CCAGAAGGTGGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTGGTTTGTACCCAATTTCTACCCCATTTCCAGAAAAGACCGATATCGA TGTTACTGAGGCTATGATTGCCCAAAAGTGGCCAAAGGATAGATTGTTTCAAGAGGCCGAAAAGTTCTTCATGTCTGTTGGTTTGTACAAGATGTTTGACAACT TCTGGAAGGACTCTATGTTGGAGAAGCCAACCGATGGTAGAAAGGTTGTTTGTCATCCAACTGCTTGGGATATGGGTAACAGAGAAGATTTTAGAATCAAGAT GTGCACGGAGGTCAACATGGACCATTTTTTGACCGCTCACCATGAAATGGGTCATAACCAATACCAAATGGCCTACAGAAACTTGTCTTACTTGTTGCGTGAT GGTGCCAACGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCTGCTACTCCAAAGCATTTGAAGGCTTTGGGTTTGTTGCCAGGTGATTTTGTT GAAGATAAGGAGACCGAAATCAACTTCTTGATGAAGCAGGCTTTGACCATTGTTGCTACTTTGCCATTTACTTACATGTTGGAGGAATGGCGTTGGCAAGTTTT TTTGGGTACTATTCCAAAGGACCAGTGGATGCAAAGATGGTGGGAAATGAAGAGAGATATGGTTGGTGTTGTTGAGCCATTGCCAAGAGATGAAACTTACTGT GATCCACCAGCTTTGTTTCATGTTTCTGGTGATTACTCTTTCATCCGTTACTTTACCAGAACCGTCTACCAATTTCAATTCCAGAAGGCTTTGTGCGAAGCCGCT GGTCATTCTGGTCCATTGTTTAAGTGTGATATTACCAACTCCACCGCCGCTGGTGATAAGTTGAGAACTATGTTGGAATTTGGTCGTTCCAAGTCCTGGACTCG TGCTTTGGAAACTATTTCTGGTAACGCTAAGATGGACTCTGCCCCATTGTTGGATTACTTTAAGGACTTGCATGTCTGGTTGATCGAAGAGAACAGAAAGAAC AACAGAAAGCCAGGTTGGAGAGCTGCTGAAGATCCATTTTCTGCT
SEQ ID NO.39:SalACE2-740(三文鱼)核苷酸序列
ATGAACAAGATGTCCTCTATTTACTCCACCGGTACTGTTTGTAAGAGAGAAGATCCATTTGACTGCCAGACTTTGGAGCCAGGTTTGGAATCTGTTA TGGCTAACATGGATTCTGACTACTACGAACGTTTGCACGTCTGGGAGGGTTGGAGAGTTGAAGTTGGTAAGAAGATGAGACCATTGTACGAAGATTACGTCGA TTTGAAGAACGAGGCTGCTAAGTTGAACGGTTACGAAGATTACGGTGATTACTGGAGATCCAACTACGAAACTATTGACGACTCTCCCTACAACTACGCCAGA GGTCAATTGATGACTGATGTTAGACATATCTACAAGGAAATCTTGCCCTTGTACAAGGAGTTGCATGCCTACGTTAGATCCAAGTTGCAGGCTAAGCATCCAG AACATATTCATCCAGAAGGTGGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTGGTTTGTACCCAATTTCTACCCCATTTCCAGAAAAG ACTGATATCGATGTTACCGACGCTATGATTGCCCAAAAGTGGCCAAAGGATAGATTGTTTCAAGAGGCTGAAAAGTTCTTCATGTCCGTCGGTTTGTACAAGA TGTTTGATAACTTTTGGAAGGACTCCATGTTGGAGAAGCCAACTGATGGTAGAAAGGTTGTTTGTCATCCAACTGCTTGGGATATGGGTAACAGAGAAGATTT TAGAATCAAGATGTGCACTGAGGTCAACATGGACCATTTTTTGACCGCCCATCATGAAATGGGTCACAACCAATACCAAATGGCTTACAGAAACTTGTCCTAC TTGTTGCGTGATGGTGCTAACGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGAGCTTGTCCGCTGCTACTCCAAAGCATTTGAAGGCTTTGGGTTTGTTGCC AGATGATTTTGTTGAAGACAAGGAGACCGAAATCAACTTCTTGATGAAGCAGGCTTTGACCATTGTCGCCACTTTGCCATTTACTTACATGTTGGAGGAGTGG AGATGGCAAGTTTTTTTGGGTACTATTCCAAAGGACCAGTGGATGCAAAGATGGTGGGAAATGAAGAGAGATATGGTTGGTGTTGTTGAGCCATTGCCAAGA GATGAAACTTACTGTGATCCACCAGCTTTGTTTCATGTTTCTGGTGATTACTCTTTCATCCGCTACTTTACCAGAACCATTTACCAGTTCCAATTCCAGAAGGCT TTGTGTGAGGCTGCTGGTCATTCTGGTCCATTGTTTAAGTGTGATATTACCAACTCCACCGCCGCTGGTGATAAGTTGAGAACTATGTTGGAATTTGGTCGTTCC AAGTCCTGGACTAGAGCTTTGGAAACTATTTCCGGTCATGCTAAGATGGATTCTGCTCCATTGTTGGATTACTTTAAGGACTTGCACGTCTGGTTGATTGAAGA GAACAGAAAGAACAACCGTAAGCCAGGTTGGAGAGCTGCTGAAGATCCATTTTCTGAAAACGCTTACAAGGTCCGTTTGTCCTTGAAGGCCGCTATGGGTGAT AAGGCTTACATTTGGAACGCTAACGAAATGTACTTGTTCAAGGCTAACATGGCCTACGCTATGAGACAATACTACTTGGAAGTTAACAAGACCGAGGTTTTGTTCACCACTGAGAACATTCACACCTACAAGGAGACCGCTAGAATTTCCTTTTACTTTGTCGTTACCGACCCAGCCAACCCAGCTGTTGTTATTCCAAAGGCTGAA GTTGAAGCTGCTATTAGATTGTCTAGAGGTAGAATTAACGACGCCTTTAAGTTGGACGATAAGACCTTGGAATTTGAGGGTTTGTTGGCTACTTTGGCCCCACC AGTTGAACAACCAGTTACTGTTTGGTTGGTTGTTTTTGGTGTTGTCATGGGTTTGGTCGTTTGCATGGGTTGTTACTTGATTATCTCTGGTTTTCGTGACCGTAAG AAGAAGTGCGCCGCTAAGGCTAAGGAAAACGCTGAAAACCCATACGGTGTTACTAACAAGACTTTTGAGAGAGAGGAAGACGAACAGACCGGTTTTCATCAC
SEQ ID NO.40:SalACE2-615(三文鱼)核苷酸序列
ATGAACAAGATGTCCTCTATTTACTCCACCGGTACTGTTTGTAAGAGAGAAGATCCATTTGACTGCCAGACTTTGGAGCCAGGTTTGGAATCTGTTATGGC TAACATGGATTCTGACTACTACGAACGTTTGCACGTCTGGGAGGGTTGGAGAGTTGAAGTTGGTAAGAAGATGAGACCATTGTACGAAGATTACGTCGATTTG AAGAACGAGGCTGCTAAGTTGAACGGTTACGAAGATTACGGTGATTACTGGAGATCCAACTACGAAACTATTGACGACTCTCCCTACAACTACGCCAGAGGTC AATTGATGACTGATGTTAGACATATCTACAAGGAAATCTTGCCCTTGTACAAGGAGTTGCATGCCTACGTTAGATCCAAGTTGCAGGCTAAGCATCCAGAACA TATTCATCCAGAAGGTGGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTGGTTTGTACCCAATTTCTACCCCATTTCCAGAAAAGACTG ATATCGATGTTACCGACGCTATGATTGCCCAAAAGTGGCCAAAGGATAGATTGTTTCAAGAGGCTGAAAAGTTCTTCATGTCCGTCGGTTTGTACAAGATGTTT GATAACTTTTGGAAGGACTCCATGTTGGAGAAGCCAACTGATGGTAGAAAGGTTGTTTGTCATCCAACTGCTTGGGATATGGGTAACAGAGAAGATTTTAGAA TCAAGATGTGCACTGAGGTCAACATGGACCATTTTTTGACCGCCCATCATGAAATGGGTCACAACCAATACCAAATGGCTTACAGAAACTTGTCCTACTTGTTG CGTGATGGTGCTAACGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGAGCTTGTCCGCTGCTACTCCAAAGCATTTGAAGGCTTTGGGTTTGTTGCCAGATGA TTTTGTTGAAGACAAGGAGACCGAAATCAACTTCTTGATGAAGCAGGCTTTGACCATTGTCGCCACTTTGCCATTTACTTACATGTTGGAGGAGTGGAGATGGC AAGTTTTTTTGGGTACTATTCCAAAGGACCAGTGGATGCAAAGATGGTGGGAAATGAAGAGAGATATGGTTGGTGTTGTTGAGCCATTGCCAAGAGATGAAA CTTACTGTGATCCACCAGCTTTGTTTCATGTTTCTGGTGATTACTCTTTCATCCGCTACTTTACCAGAACCATTTACCAGTTCCAATTCCAGAAGGCTTTGTGTGA GGCTGCTGGTCATTCTGGTCCATTGTTTAAGTGTGATATTACCAACTCCACCGCCGCTGGTGATAAGTTGAGAACTATGTTGGAATTTGGTCGTTCCAAGTCCT GGACTAGAGCTTTGGAAACTATTTCCGGTCATGCTAAGATGGATTCTGCTCCATTGTTGGATTACTTTAAGGACTTGCACGTCTGGTTGATTGAAGAGAACAGA AAGAACAACCGTAAGCCAGGTTGGAGAGCTGCTGAAGATCCATTTTCTGAAAACGCTTACAAGGTCCGTTTGTCCTTGAAGGCCGCTATGGGTGATAAGGCTT ACATTTGGAACGCTAACGAAATGTACTTGTTCAAGGCTAACATGGCCTACGCTATGAGACAATACTACTTGGAAGTTAACAAGACCGAGGTTTTGTTCACCAC TGAGAACATTCACACCTACAAGGAGACCGCTAGAATTTCCTTTTACTTTGTCGTTACCGACCCAGCCAACCCAGCTGTTGTTATTCCAAAGGCTGAAGTTGAAG CTGCTATTAGATTGTCTAGAGGTAGAATTAACGACGCCTTTAAGTTGGACGATAAGACCTTGGAATTTGAGGGTTTGTTGGCTACTTTGGCCCCACCAGTTGAA CAACCAGTTACT
SEQ ID NO.41:StACE2-740(亚东鲑)核苷酸序列
TCTGACTTGGAAAGAAGAGCCCAAGAATTTTTGGATACCTTTGACGGTAACGCCACCCATTTGATGTACCAATACTCTTTGGCTTCTTGGGCTTACAACAC TGATATTTCTCAAGAGAACTTGGACAAGTTGGGTGTTCAATCCGCTATTTGGGGTGAATACTACTCTAAGGTTTCTAAGGAATCCGAGAACTTCCCAATTGACC AAATTTCTGATCCATTGATCAAGTTGCAGTTGACGTCCTTGCAGGACAAGGGTTCTGGTGCTTTGTCTGCTGATAAGGCTGCTCATTTGAACAAGGTTATGAAC AAGATGTCCTCCATCTACTCCACCGGTACTGTCTGTAAGAGAGAAGATCCATTTGATTGCCAGACCTTGGAGCCAGGTTTGGAATCTGTTATGGCTAACATGGA TTCTGACTACTACGAAAGATTGCACGTTTGGGAAGGTTGGAGAGTTGAAGTTGGTAAGAAGATGAGACCATTGTACGAAGATTACGTCGATTTGAAGAACGA GGCCGCTAAGTTGAACGGTTACGAAGATTACGGTGATTACTGGAGATCCAACTACGAAACTATTGACGACTCCCCATACAACTACGCCAGAGGTCAATTGATG ACTGATGTTAGAAGAATCTACAAGGAGATATTGCCCTTGTACAAGGAGTTGCATGCTTACGTTAGATCCAAGTTGCAAGCCAAGCATCCAGAACATATTCACC CAGAAGGTGGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTGGTTTGTACCCAATTTCTACCCCATTTCCAGAAAAGACTGATATCGAT GTTACCGACGCCATGATCGCTCAAAAGTGGCCAAAGGATAGATTGTTTCAAGAGGCTGAAAAGTTCTTTATGTCCGTCGGTTTGTACAAGATGTTCGATAACTT TTGGAAGGACTCCATGTTGGAGAAGCCAACTGATGGTAGAAAGGTTGTTTGTCATCCAACTGCTTGGGATATGGGTAACAGAGAAGATTTTAGAATCAAGATG TGCACCGAGGTCAACATGGATCACTTTTTGACTGCCCACCATGAGATGGGTCATAACCAATACCAAATGGCTTACAGAAACTTGTCCTACTTGTTGAGAGATG GTGCTAACGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCCGCTACTCCAAAGCATTTGAAGGCTTTGGGTTTGTTGCCAGATGATTTTGTTG AAGACAAGGAGACCGAGATCAACTTTTTGATGAAGCAGGCCTTGACTATTGTCGCCACTTTGCCATTTACTTACATGTTGGAGGAATGGAGATGGCAAGTTTT TTTGGGTACTATTCCAAAGGACCAGTGGATGCAAAGATGGTGGGAAATGAAGAGAGATATGGTTGGTGTTGTTGAGCCATTGCCAAGAGATGAAACTTACTGT GATCCACCAGCTTTGTTTCATGTTTCTGGTGATTACTCTTTCATCCGTTACTTCACTCGTACTATCTACCAGTTTCAATTCCAGAAGGCTTTGTGTGAAGCCGCTG GTCATTCTGGTCCATTGTTTAAGTGTGATATTACCAACTCCACCGCCGCCGGTGATAAGTTGAGAACTATGTTGGAATTTGGTCGTTCCAAGTCCTGGACTAGA GCTTTGGAAACTATTTCCGGTCATGCTAAGATGGATTCCGCCCCATTGTTGGATTACTTTAAGGATTTGCATGTCTGGTTGATCGAGGAGAACCGTAAGAACAA CAGAAAGCCAGGTTGGAGAGCTGCTGAAGATCCATTTTCTGAAAACGCTTACAAGGTCAGATTGTCTTTGAAGGCTGCTATGGGTGATAAGGCTTACATTTGG AACGGTAACGAAATGTACTTGTTCAAGGCTAACATGGCCTACGCTATGAGACAATACTACTTGGAAGTTAACAAGACCGAGGTTTTGTTCACCACTGAGAACA TCCATACTTACAAGGAGACTGCTAGAATTTCCTTCTACTTCGTCGTTACTGATCCAGCCAACCCAGCTGTTGTTATTCCAAAGGCTGAAGTTGAAGCTGCTATT AGATTGTCTAGAGGCAGAATTAACGACGCCTTTAAGTTGGACGATAAGACTTTGGAGTTCGAGGGTTTGTTGGCCACTTTGGCTCCACCAGTTGAACAACCAGTTACT
SEQ ID NO.42:StACE2-615(亚东鲑)核苷酸序列
TCTGACTTGGAAAGAAGAGCCCAAGAATTTTTGGATACCTTTGACGGTAACGCCACCCATTTGATGTACCAATACTCTTTGGCTTCTTGGGCTTACAACAC TGATATTTCTCAAGAGAACTTGGACAAGTTGGGTGTTCAATCCGCTATTTGGGGTGAATACTACTCTAAGGTTTCTAAGGAATCCGAGAACTTCCCAATTGACC AAATTTCTGATCCATTGATCAAGTTGCAGTTGACGTCCTTGCAGGACAAGGGTTCTGGTGCTTTGTCTGCTGATAAGGCTGCTCATTTGAACAAGGTTATGAAC AAGATGTCCTCCATCTACTCCACCGGTACTGTCTGTAAGAGAGAAGATCCATTTGATTGCCAGACCTTGGAGCCAGGTTTGGAATCTGTTATGGCTAACATGGA TTCTGACTACTACGAAAGATTGCACGTTTGGGAAGGTTGGAGAGTTGAAGTTGGTAAGAAGATGAGACCATTGTACGAAGATTACGTCGATTTGAAGAACGA GGCCGCTAAGTTGAACGGTTACGAAGATTACGGTGATTACTGGAGATCCAACTACGAAACTATTGACGACTCCCCATACAACTACGCCAGAGGTCAATTGATG ACTGATGTTAGAAGAATCTACAAGGAGATATTGCCCTTGTACAAGGAGTTGCATGCTTACGTTAGATCCAAGTTGCAAGCCAAGCATCCAGAACATATTCACC CAGAAGGTGGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTGGTTTGTACCCAATTTCTACCCCATTTCCAGAAAAGACTGATATCGAT GTTACCGACGCCATGATCGCTCAAAAGTGGCCAAAGGATAGATTGTTTCAAGAGGCTGAAAAGTTCTTTATGTCCGTCGGTTTGTACAAGATGTTCGATAACTT TTGGAAGGACTCCATGTTGGAGAAGCCAACTGATGGTAGAAAGGTTGTTTGTCATCCAACTGCTTGGGATATGGGTAACAGAGAAGATTTTAGAATCAAGATG TGCACCGAGGTCAACATGGATCACTTTTTGACTGCCCACCATGAGATGGGTCATAACCAATACCAAATGGCTTACAGAAACTTGTCCTACTTGTTGAGAGATG GTGCTAACGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCCGCTACTCCAAAGCATTTGAAGGCTTTGGGTTTGTTGCCAGATGATTTTGTTG AAGACAAGGAGACCGAGATCAACTTTTTGATGAAGCAGGCCTTGACTATTGTCGCCACTTTGCCATTTACTTACATGTTGGAGGAATGGAGATGGCAAGTTTT TTTGGGTACTATTCCAAAGGACCAGTGGATGCAAAGATGGTGGGAAATGAAGAGAGATATGGTTGGTGTTGTTGAGCCATTGCCAAGAGATGAAACTTACTGT GATCCACCAGCTTTGTTTCATGTTTCTGGTGATTACTCTTTCATCCGTTACTTCACTCGTACTATCTACCAGTTTCAATTCCAGAAGGCTTTGTGTGAAGCCGCTG GTCATTCTGGTCCATTGTTTAAGTGTGATATTACCAACTCCACCGCCGCCGGTGATAAGTTGAGAACTATGTTGGAATTTGGTCGTTCCAAGTCCTGGACTAGA GCTTTGGAAACTATTTCCGGTCATGCTAAGATGGATTCCGCCCCATTGTTGGATTACTTTAAGGATTTGCATGTCTGGTTGATCGAGGAGAACCGTAAGAACAA CAGAAAGCCAGGTTGGAGAGCTGCTGAAGATCCATTTTCTGAA
SEQ ID NO.43:MlACE2-740(水貂)核苷酸序列
CAGTCTACTACCGAAGATTTGGCTAAGACTTTCTTGGAAAAGTTCAACTACGAGGCCGAAGAATTGTCTTACCAAAACTCTTTGGCTTCCTGGAACTACAA CACTAACATTACTGATGAGAACATCCAGAAGATGAACATCGCCGGTGCCAAGTGGTCTGCTTTTTACGAAGAAGAATCTCAGCATGCCAAGACCTACCCATTG GAAGAAATTCAGGACCCAATTATTAAGCGTCAGTTGAGAGCCTTGCAACAGTCTGGTTCTTCTGTTTTGTCTGCTGATAAGAGAGAACGCTTGAACACTATTTT GAACGCCATGTCCACTATCTACTCCACTGGTAAGGCTTGTAACCCAAACAACCCACAAGAATGTTTGTTGTTGGAACCAGGTTTGGATGATATTATGGAAAAC TCCAAGGACTACAACGAGCGTTTGTGGGCTTGGGAAGGTTGGCGTTCTGAAGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTCGCTTTGAAGAACG AAATGGCCAGAGCTAACAACTACGAAGATTACGGTGATTACTGGAGAGGTGATTACGAAGAAGAATGGGCTGATGGTTACTCTTACTCTAGAAACCAATTGA TCGAGGACGTCGAGCATACTTTTACTCAAATCAAGCCATTGTACGAGCACTTGCACGCTTACGTTAGAGCTAAGTTGATGGATGCTTACCCATCTAGAATTTCC CCAACTGGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGATGGTCCCATTTGGTCAGAAGCCAAACATTGA CGTTACTGACGCTATGGTTAACCAATCTTGGGATGCTAGAAGAATTTTCGAGGAGGCTGAAACCTTTTTTGTTTCCGTTGGTTTGCCAAACATGACCGAAGGTT TTTGGCAAAACTCTATGTTGACTGAGCCAGGTGATAACAGAAAGGTTGTTTGTCATCCAACTGCCTGGGATTTGGGTAAGAGAGATTTTAGAATTAAGATGTG CACCAAGGTCACCATGGACGACTTCTTGACTGCTCATCATGAAATGGGTCATATTCAATACGACATGGCCTACGCTGAACAACCATTTTTGTTGAGAAACGGT GCTAACGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCCGCTACTCCAAACCATTTGAAGAACATTGGTTTGTTGCCCCCAGATTTTTCCGA AGACTCTGAAACTGACATTAACTTCTTGTTGAAGCAAGCCTTGACCATCGTTGGTACTTTGCCATTTACTTACATGTTGGAGAAGTGGCGTTGGATGGTTTTTA AGGGTGAAATTCCAAAGGAGCAGTGGATGCAAAAGTGGTGGGAAATGAAGAGAGATATTGTCGGTGTTGTTGAGCCATTGCCACATGATGAAACTTACTGTG ATCCAGCTGCTTTGTTTCATGTTGCTAACGATTACTCTTTCATCCGTTACTACACCCGTACTATCTACCAGTTTCAATTTCAGGAAGCCTTGTGTCAAATTGCCA AGCACGAAGGTCCATTGTACAAGTGTGATATTTCTAACTCCAGAGAGGCCGGTCAAAAGTTGCATGAAATGTTGTCTTTGGGTCGTTCTAAGCCATGGACTTTT GCTTTGGAAAGAGTTGTTGGTGCTAAGACTATGGATGTTAGACCATTGTTGAACTACTTCGAGCCATTGTTTACTTGGTTGAAGGAGCAGAACAGAAACTCCTT CGTCGGTTGGAACACTGATTGGTCTCCATACGCTGATCAATCCATTAAGGTCCGTATCTCTTTGAAGTCTGCTTTGGGTGAAAAGGCTTACGAATGGAACGATA ACGAAATGTACTTTTTCCAGTCCTCCATCGCTTACGCTATGAGAGAATACTTTTCCAAGGTCAAGAACCAGACTATTCCATTTGTTGGTAAGGACGTTAGAGTC TCCGATTTGAAGCCAAGAATTTCCTTTAACTTCATCGTCACCTCCCCAGAGAACATGTCTGATATTATTCCAAGAGCCGATGTCGAAGAGGCCATTCGTAAGTC TAGAGGTAGAATTAACGATGCCTTTCGTTTGGACGATAACTCCTTGGAATTTTTGGGTATCCAGCCAACCTTGGAGCCACCATACCAACCACCAGTTACT
SEQ ID NO.44:MlACE2-615(水貂)核苷酸序列
CAGTCTACTACCGAAGATTTGGCTAAGACTTTCTTGGAAAAGTTCAACTACGAGGCCGAAGAATTGTCTTACCAAAACTCTTTGGCTTCCTGGAACT ACAACACTAACATTACTGATGAGAACATCCAGAAGATGAACATCGCCGGTGCCAAGTGGTCTGCTTTTTACGAAGAAGAATCTCAGCATGCCAAGACCTACCC ATTGGAAGAAATTCAGGACCCAATTATTAAGCGTCAGTTGAGAGCCTTGCAACAGTCTGGTTCTTCTGTTTTGTCTGCTGATAAGAGAGAACGCTTGAACACTA TTTTGAACGCCATGTCCACTATCTACTCCACTGGTAAGGCTTGTAACCCAAACAACCCACAAGAATGTTTGTTGTTGGAACCAGGTTTGGATGATATTATGGAA AACTCCAAGGACTACAACGAGCGTTTGTGGGCTTGGGAAGGTTGGCGTTCTGAAGTTGGTAAGCAATTGAGACCATTGTACGAAGAATACGTCGCTTTGAAGA ACGAAATGGCCAGAGCTAACAACTACGAAGATTACGGTGATTACTGGAGAGGTGATTACGAAGAAGAATGGGCTGATGGTTACTCTTACTCTAGAAACCAAT TGATCGAGGACGTCGAGCATACTTTTACTCAAATCAAGCCATTGTACGAGCACTTGCACGCTTACGTTAGAGCTAAGTTGATGGATGCTTACCCATCTAGAATT TCCCCAACTGGTTGTTTGCCAGCTCATTTGTTGGGTGATATGTGGGGTAGATTTTGGACTAACTTGTACCCATTGATGGTCCCATTTGGTCAGAAGCCAAACATTGACGTTACTGACGCTATGGTTAACCAATCTTGGGATGCTAGAAGAATTTTCGAGGAGGCTGAAACCTTTTTTGTTTCCGTTGGTTTGCCAAACATGACCGAAG GTTTTTGGCAAAACTCTATGTTGACTGAGCCAGGTGATAACAGAAAGGTTGTTTGTCATCCAACTGCCTGGGATTTGGGTAAGAGAGATTTTAGAATTAAGAT GTGCACCAAGGTCACCATGGACGACTTCTTGACTGCTCATCATGAAATGGGTCATATTCAATACGACATGGCCTACGCTGAACAACCATTTTTGTTGAGAAAC GGTGCTAACGAAGGTTTTCATGAAGCTGTTGGTGAAATTATGTCCTTGTCTGCCGCTACTCCAAACCATTTGAAGAACATTGGTTTGTTGCCCCCAGATTTTTCC GAAGACTCTGAAACTGACATTAACTTCTTGTTGAAGCAAGCCTTGACCATCGTTGGTACTTTGCCATTTACTTACATGTTGGAGAAGTGGCGTTGGATGGTTTT TAAGGGTGAAATTCCAAAGGAGCAGTGGATGCAAAAGTGGTGGGAAATGAAGAGAGATATTGTCGGTGTTGTTGAGCCATTGCCACATGATGAAACTTACTG TGATCCAGCTGCTTTGTTTCATGTTGCTAACGATTACTCTTTCATCCGTTACTACACCCGTACTATCTACCAGTTTCAATTTCAGGAAGCCTTGTGTCAAATTGC CAAGCACGAAGGTCCATTGTACAAGTGTGATATTTCTAACTCCAGAGAGGCCGGTCAAAAGTTGCATGAAATGTTGTCTTTGGGTCGTTCTAAGCCATGGACTTTTGCTTTGGAAAGAGTTGTTGGTGCTAAGACTATGGATGTTAGACCATTGTTGAACTACTTCGAGCCATTGTTTACTTGGTTGAAGGAGCAGAACAGAAACTC CTTCGTCGGTTGGAACACTGATTGGTCTCCATACGCTGAT
SEQ ID NO.45:VvACE2-740(狐狸)核苷酸序列
CAGTCAACAGAAGATTTAGTGAATACGTTTCTCGAGAAGTTTAATTACGAGGCTGAAGAGTTATCGTATCAGAGTTCTTTGGCCAGTTGGGACTATA ATACGAATATTTCCGACGAAAACGTACAGAAAATGAACAATGCCGGAGCAAAGTGGTCGGCATTCTATGAAGAGCAGAGTAAACTCGCCAAAACTTACCCGC TCGAAGAGATACAAGATTCTACAGTGAAGCGTCAACTAAGAGCATTACAACATTCAGGTTCTTCTGTTCTATCTGCTGACAAGAACCAAAGATTAAATACCAT TTTGAACTCTATGTCCACTATATATTCCACTGGAAAAGCATGTAATCCTTCGAACCCGCAAGAGTGTTTACTACTGGAGCCCGGCCTCGATGATATTATGGAGA ACAGCAAAGATTACAACGAGCGCCTTTGGGCTTGGGAGGGGTGGCGGTCAGAAGTAGGAAAACAGCTAAGGCCACTCTACGAGGAGTACGTCGCACTTAAGA ATGAAATGGCCAGGGCGAACAATTATGAGGACTATGGAGACTACTGGCGTGGGGATTATGAAGAGGAGTGGGAGAACGGGTATAACTACAGTCGCAATCAG CTAATAGATGACGTGGAGCACACTTTCACCCAAATCATGCCCCTGTACCAGCACTTACACGCATACGTTCGCACGAAGCTAATGGATACGTACCCGTCCTATA TATCGCCTACCGGGTGCTTGCCCGCCCACCTGCTTGGTGATATGTGGGGTCGTTTTTGGACTAATTTGTATCCCCTCACGGTACCTTTTGGTCAGAAACCGAAC ATTGATGTCACTAACGCGATGGTCAACCAGTCGTGGGATGCGAGAAAAATCTTCAAAGAAGCGGAAAAGTTCTTCGTAAGCGTTGGGCTGCCAAACATGACT CAGGGCTTTTGGGAAAACAGCATGCTTACTGAACCCTCCGACTCGCGTAAGGTCGTGTGCCATCCGACAGCTTGGGACCTTGGAAAAGGAGATTTTCGAATTA AAATGTGCACAAAAGTCACCATGGATGACTTCCTCACGGCACACCATGAGATGGGGCACATACAATACGATATGGCATACGCCGCTCAGCCATTCTTGCTGCG AAATGGCGCCAATGAAGGTTTCCATGAAGCGGTCGGCGAAATCATGAGCCTTAGTGCTGCCACACCAAACCACCTAAAAAATATCGGCCTATTACCTCCTTCG TTTTTTGAAGATAGTGAAACGGAAATAAATTTCCTGTTAAAACAGGCACTTACAATCGTAGGCACACTGCCTTTCACCTATATGTTAGAAAAATGGCGGTGGA TGGTGTTTAAAGGTGAAATCCCGAAGGACCAATGGATGAAGACTTGGTGGGAGATGAAGCGCAATATTGTGGGAGTAGTGGAGCCAGTCCCTCATGACGAAA CATATTGTGACCCGGCCAGCCTTTTTCATGTTGCTAACGACTATTCCTTTATCCGATACTATACGAGGACCATTTACCAATTCCAATTCCAGGAAGCGTTGTGCC AAATAGCTAAGCACGAGGGACCACTTCACAAGTGTGACATTTCTAATTCCAGTGAGGCTGGGCAAAAGCTACTGGAAATGCTAAAACTGGGTAAGTCAAAGC CTTGGACGTATGCCTTGGAAATCGTCGTAGGGGCCAAAAATATGGACGTGCGACCGCTGCTAAACTACTTTGAACCATTGTTTACTTGGTTGAAGGAGCAAAA CAGAAATTCCTTTGTTGGCTGGAATACAGACTGGAGCCCCTATGCAGATCAGTCGATCAAGGTAAGAATAAGTCTGAAGAGCGCGTTGGGCGAAAAAGCTTA TGAATGGAATAATAACGAGATGTACCTTTTCCGGTCGTCTATTGCGTACGCGATGCGACGATACTTTTCAGAGGTGAAGAAACAGACCATCCCCTTTGTTGAG GACAACGTTTGGGTTTCTGACCTTAAACCGAGGATATCATTTAATTTCTTTGTCACCTCACCAGGGAACGTTTCAGACATTATTCCGCGGACAGAAGTAGAGAA GGCGATACGGATGTATCGTGGTCGCATAAATGATGTGTTCAGGTTAGATGATAACTCTCTCGAATTTTTAGGCATACAACCCACCTTGGGTCCTAGTTACGAGC CACCCGTTACCATC
SEQ ID NO.46:VvACE2-615(狐狸)核苷酸序列
CAGTCAACAGAAGATTTAGTGAATACGTTTCTCGAGAAGTTTAATTACGAGGCTGAAGAGTTATCGTATCAGAGTTCTTTGGCCAGTTGGGACTATA ATACGAATATTTCCGACGAAAACGTACAGAAAATGAACAATGCCGGAGCAAAGTGGTCGGCATTCTATGAAGAGCAGAGTAAACTCGCCAAAACTTACCCGC TCGAAGAGATACAAGATTCTACAGTGAAGCGTCAACTAAGAGCATTACAACATTCAGGTTCTTCTGTTCTATCTGCTGACAAGAACCAAAGATTAAATACCAT TTTGAACTCTATGTCCACTATATATTCCACTGGAAAAGCATGTAATCCTTCGAACCCGCAAGAGTGTTTACTACTGGAGCCCGGCCTCGATGATATTATGGAGA ACAGCAAAGATTACAACGAGCGCCTTTGGGCTTGGGAGGGGTGGCGGTCAGAAGTAGGAAAACAGCTAAGGCCACTCTACGAGGAGTACGTCGCACTTAAGA ATGAAATGGCCAGGGCGAACAATTATGAGGACTATGGAGACTACTGGCGTGGGGATTATGAAGAGGAGTGGGAGAACGGGTATAACTACAGTCGCAATCAG CTAATAGATGACGTGGAGCACACTTTCACCCAAATCATGCCCCTGTACCAGCACTTACACGCATACGTTCGCACGAAGCTAATGGATACGTACCCGTCCTATA TATCGCCTACCGGGTGCTTGCCCGCCCACCTGCTTGGTGATATGTGGGGTCGTTTTTGGACTAATTTGTATCCCCTCACGGTACCTTTTGGTCAGAAACCGAAC ATTGATGTCACTAACGCGATGGTCAACCAGTCGTGGGATGCGAGAAAAATCTTCAAAGAAGCGGAAAAGTTCTTCGTAAGCGTTGGGCTGCCAAACATGACT CAGGGCTTTTGGGAAAACAGCATGCTTACTGAACCCTCCGACTCGCGTAAGGTCGTGTGCCATCCGACAGCTTGGGACCTTGGAAAAGGAGATTTTCGAATTA AAATGTGCACAAAAGTCACCATGGATGACTTCCTCACGGCACACCATGAGATGGGGCACATACAATACGATATGGCATACGCCGCTCAGCCATTCTTGCTGCG AAATGGCGCCAATGAAGGTTTCCATGAAGCGGTCGGCGAAATCATGAGCCTTAGTGCTGCCACACCAAACCACCTAAAAAATATCGGCCTATTACCTCCTTCG TTTTTTGAAGATAGTGAAACGGAAATAAATTTCCTGTTAAAACAGGCACTTACAATCGTAGGCACACTGCCTTTCACCTATATGTTAGAAAAATGGCGGTGGA TGGTGTTTAAAGGTGAAATCCCGAAGGACCAATGGATGAAGACTTGGTGGGAGATGAAGCGCAATATTGTGGGAGTAGTGGAGCCAGTCCCTCATGACGAAA CATATTGTGACCCGGCCAGCCTTTTTCATGTTGCTAACGACTATTCCTTTATCCGATACTATACGAGGACCATTTACCAATTCCAATTCCAGGAAGCGTTGTGCC AAATAGCTAAGCACGAGGGACCACTTCACAAGTGTGACATTTCTAATTCCAGTGAGGCTGGGCAAAAGCTACTGGAAATGCTAAAACTGGGTAAGTCAAAGC CTTGGACGTATGCCTTGGAAATCGTCGTAGGGGCCAAAAATATGGACGTGCGACCGCTGCTAAACTACTTTGAACCATTGTTTACTTGGTTGAAGGAGCAAAA CAGAAATTCCTTTGTTGGCTGGAATACAGACTGGAGCCCCTATGCAGAT
SEQ ID NO.47:EcACE2-740(马)核苷酸序列
CAGTCCACTACTGAGGACCTAGCAAAGACGTTCCTTGAAAAGTTCAATAGTGAGGCGGAAGAGTTGTCACACCAGTCTTCATTAGCATCCTGGTCG TACAACACAAACATCACCGATGAAAACGTTCAAAAGATGAATGAAGCGGGAGCGAGATGGTCTGCTTTTTACGAGGAGCAATGTAAGCTGGCCAAAACCTAC CCATTGGAGGAAATACAAAACCTCACAGTTAAACGTCAACTACAAGCCTTGCAACAAAGTGGTTCTTCAGTACTTTCCGCCGATAAAAGCAAGCGACTAAACG AGATATTAAATACTATGTCCACAATTTACTCCACAGGAAAGGTCTGCAACCCTAGCAATCCGCAGGAATGTCTACTTCTGGAGCCGGGGCTGGACGCAATAAT GGAAAACTCCAAAGACTATAACCAGAGGCTATGGGCCTGGGAAGGATGGCGGTCAGAGGTAGGCAAACAACTCCGCCCGTTGTACGAAGAGTACGTTGTGCT TAAAAATGAAATGGCACGAGCAAACAATTATGAAGATTACGGGGATTATTGGCGTGGAGATTACGAGGCAGAGGGCCCGAGCGGTTACGATTACTCACGGGA TCAGCTGATCGAAGACGTAGAACGAACGTTCGCTGAAATCAAGCCACTCTACGAGCACTTACATGCGTATGTTAGAGCGAAGTTGATGGACACATATCCATCT CACATCAACCCAACCGGTTGCCTTCCGGCCCATTTATTGGGTGACATGTGGGGCAGATTTTGGACTAACTTGTATAGCTTAACGGTACCCTTCGGTCAGAAACC CAATATTGATGTGACGGATGCAATGGTTGATCAAAGCTGGGACGCTAAAAGGATTTTCGAAGAAGCTGAGAAGTTCTTCGTGTCGGTCGGGCTCCCAAATATG ACTCAAGGGTTTTGGGAGAATAGCATGTTGACGGAGCCTGGCGACGGCCGGAAAGTCGTTTGCCACCCTACCGCATGGGACCTAGGGAAAGGAGATTTCCGA ATTAAGATGTGCACTAAGGTCACCATGGACGATTTCCTCACAGCTCATCATGAGATGGGCCACATTCAGTATGACATGGCCTATGCAGTACAGCCCTACCTACTGCGCAACGGTGCAAATGAGGGCTTTCACGAGGCCGTTGGCGAAATAATGTCATTGAGCGCGGCCACCCCCAATCATCTAAAGGCCATTGGACTTTTACCTCC TGATTTCTACGAAGATTCTGAAACTGAGATTAACTTCCTCTTAAAACAGGCTTTAACGATAGTGGGAACGCTACCATTTACATATATGCTGGAAAAGTGGAGA TGGATGGTCTTTAAAGGTGAAATTCCTAAAGAGGAGTGGATGAAGAAATGGTGGGAGATGAAGCGTGAGATTGTGGGGGTGGTTGAGCCAGTACCACATGAC GAAACATACTGTGATCCAGCAGCCTTGTTTCACGTCGCGAATGACTACTCGTTTATACGTTATTATACGCGCACTATCTATCAATTCCAATTTCAGGAAGCGCT GTGCCAGACTGCTAAACACGAAGGACCGCTTCACAAGTGTGACATCAGCAATTCCACCGAAGCTGGTCAGAAGTTGCTTCAAATGCTCTCGTTAGGAAAATCC GAACCCTGGACCTTAGCGCTCGAGCGCATCGTGGGGGTGAAAAACATGGATGTTCGGCCGTTACTTAACTATTTTGAGCCCCTGTTCACCTGGCTGAAAGATC AGAATAAAAACAGTTTCGTGGGCTGGAGTACAAATTGGTCTCCCTACGCTGATCAATCTATCAAAGTACGGATATCGCTAAAGAGTGCGCTGGGTGAAAAGA GTTATGAATGGAATGATAACGAGATGTACCTATTTCAGTCCAGTGTTGCCTATGCTATGAGGGTCTACTTCCTTAAAGCGAAGAATCAAACTATACTGTTTGGC GAGGAAGACGTCTGGGTCTCTGATTTAAAGCCGCGAATATCGTTTAATTTCTTTGTAACATCGCCGAAGAACGCATCTGACATAATACCCAGGACCGACGTAG AAGAGGCGATCCGTATGAGTAGGTCTCGCATTAACGACGCTTTTAGATTAGACGATAATACGCTCGAGTTTTTAGGTATTCAACCTACTCTTGGGCCTCCTTAT CAGCCCCCTGTAACGGTT
SEQ ID NO.48:EcACE2-615(马)核苷酸序列
CAGTCCACTACTGAGGACCTAGCAAAGACGTTCCTTGAAAAGTTCAATAGTGAGGCGGAAGAGTTGTCACACCAGTCTTCATTAGCATCCTGGTCG TACAACACAAACATCACCGATGAAAACGTTCAAAAGATGAATGAAGCGGGAGCGAGATGGTCTGCTTTTTACGAGGAGCAATGTAAGCTGGCCAAAACCTAC CCATTGGAGGAAATACAAAACCTCACAGTTAAACGTCAACTACAAGCCTTGCAACAAAGTGGTTCTTCAGTACTTTCCGCCGATAAAAGCAAGCGACTAAACG AGATATTAAATACTATGTCCACAATTTACTCCACAGGAAAGGTCTGCAACCCTAGCAATCCGCAGGAATGTCTACTTCTGGAGCCGGGGCTGGACGCAATAAT GGAAAACTCCAAAGACTATAACCAGAGGCTATGGGCCTGGGAAGGATGGCGGTCAGAGGTAGGCAAACAACTCCGCCCGTTGTACGAAGAGTACGTTGTGCT TAAAAATGAAATGGCACGAGCAAACAATTATGAAGATTACGGGGATTATTGGCGTGGAGATTACGAGGCAGAGGGCCCGAGCGGTTACGATTACTCACGGGA TCAGCTGATCGAAGACGTAGAACGAACGTTCGCTGAAATCAAGCCACTCTACGAGCACTTACATGCGTATGTTAGAGCGAAGTTGATGGACACATATCCATCT CACATCAACCCAACCGGTTGCCTTCCGGCCCATTTATTGGGTGACATGTGGGGCAGATTTTGGACTAACTTGTATAGCTTAACGGTACCCTTCGGTCAGAAACC CAATATTGATGTGACGGATGCAATGGTTGATCAAAGCTGGGACGCTAAAAGGATTTTCGAAGAAGCTGAGAAGTTCTTCGTGTCGGTCGGGCTCCCAAATATG ACTCAAGGGTTTTGGGAGAATAGCATGTTGACGGAGCCTGGCGACGGCCGGAAAGTCGTTTGCCACCCTACCGCATGGGACCTAGGGAAAGGAGATTTCCGA ATTAAGATGTGCACTAAGGTCACCATGGACGATTTCCTCACAGCTCATCATGAGATGGGCCACATTCAGTATGACATGGCCTATGCAGTACAGCCCTACCTACTGCGCAACGGTGCAAATGAGGGCTTTCACGAGGCCGTTGGCGAAATAATGTCATTGAGCGCGGCCACCCCCAATCATCTAAAGGCCATTGGACTTTTACCTCC TGATTTCTACGAAGATTCTGAAACTGAGATTAACTTCCTCTTAAAACAGGCTTTAACGATAGTGGGAACGCTACCATTTACATATATGCTGGAAAAGTGGAGA TGGATGGTCTTTAAAGGTGAAATTCCTAAAGAGGAGTGGATGAAGAAATGGTGGGAGATGAAGCGTGAGATTGTGGGGGTGGTTGAGCCAGTACCACATGAC GAAACATACTGTGATCCAGCAGCCTTGTTTCACGTCGCGAATGACTACTCGTTTATACGTTATTATACGCGCACTATCTATCAATTCCAATTTCAGGAAGCGCT GTGCCAGACTGCTAAACACGAAGGACCGCTTCACAAGTGTGACATCAGCAATTCCACCGAAGCTGGTCAGAAGTTGCTTCAAATGCTCTCGTTAGGAAAATCC GAACCCTGGACCTTAGCGCTCGAGCGCATCGTGGGGGTGAAAAACATGGATGTTCGGCCGTTACTTAACTATTTTGAGCCCCTGTTCACCTGGCTGAAAGATC AGAATAAAAACAGTTTCGTGGGCTGGAGTACAAATTGGTCTCCCTACGCTGAT
SEQ ID NO.49:hACE2-740(人)氨基酸序列
QSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILN TMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIED VEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENS MLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEIN FLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKC DISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSV AYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS
SEQ ID NO.50:hACE2-615(人)氨基酸序列
QSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILN TMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIED VEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENS MLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEIN FLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKC DISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYAD
SEQ ID NO.51:AtACE2-740(老虎)氨基酸序列
STTEELAKTFLEKFNHEAEELSYQSSLASWNYNTNITDENVQKMNEAGAKWSAFYEEQSKLAETYPLAEIHNTTVKRQLQALQQSGSSVLSADKSQRLNTILNA MSTIYSTGKACNPNNPQECLLLEPGLDDIMENSKDYNERLWAWEGWRAEVGKQLRPLYEEYVALKNEMARANNYEDYGDYWRGDYEEEWTDGYNYSRSQLIKD VEHTFTQIKPLYQHLHAYVRAKLMDSYPSRISPTGCLPAHLLGDMWGRFWTNLYPLTVPFGQKPNIDVTDAMVNQSWDARRIFKEAEKFFVSVGLPNMTQGFWENS MLTEPGNSQKVVCHPTAWDLGKGDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAVQPFLLRNGANEGFHEAVGEIMSLSAATPNHLKTIGLLPPGFSEDSETEIN FLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKEQWMQKWWEMKREIVGVVEPVPHDETYCDPASLFHVANDYSFIRYYTRTIYQFQFQEALCRIAKHEGPLHKCD ISNSSEAGKKLLQMLTLGKSKPWTLALEHVVGEKNMNVTPLLKYFEPLFTWLKEQNRNSFVGWNTDWRPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVA YAMREYFSKVKNQTIPFVEDNVWVSNLKPRISFNFFVTASKNVSDVIPRREVEEAIRMSRSRINDAFRLDDNSLEFLGIQPTLSPPYQPPVT
SEQ ID NO.52:AtACE2-615(老虎)氨基酸序列
STTEELAKTFLEKFNHEAEELSYQSSLASWNYNTNITDENVQKMNEAGAKWSAFYEEQSKLAETYPLAEIHNTTVKRQLQALQQSGSSVLSADKSQRLNTILNA MSTIYSTGKACNPNNPQECLLLEPGLDDIMENSKDYNERLWAWEGWRAEVGKQLRPLYEEYVALKNEMARANNYEDYGDYWRGDYEEEWTDGYNYSRSQLIKD VEHTFTQIKPLYQHLHAYVRAKLMDSYPSRISPTGCLPAHLLGDMWGRFWTNLYPLTVPFGQKPNIDVTDAMVNQSWDARRIFKEAEKFFVSVGLPNMTQGFWENS MLTEPGNSQKVVCHPTAWDLGKGDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAVQPFLLRNGANEGFHEAVGEIMSLSAATPNHLKTIGLLPPGFSEDSETEIN FLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKEQWMQKWWEMKREIVGVVEPVPHDETYCDPASLFHVANDYSFIRYYTRTIYQFQFQEALCRIAKHEGPLHKCD ISNSSEAGKKLLQMLTLGKSKPWTLALEHVVGEKNMNVTPLLKYFEPLFTWLKEQNRNSFVGWNTDWRPYAD
SEQ ID NO.53:BtACE2-740(牛)氨基酸序列
STTEEQAKTFLEKFNHEAEDLSYQSSLASWNYNTNITDENVQKMNEARAKWSAFYEEQSRMAKTYSLEEIQNLTLKRQLKALQHSGTSALSAEKSKRLNTILNK MSTIYSTGKVLDPNTQECLALEPGLDDIMENSRDYNRRLWAWEGWRAEVGKQLRPLYEEYVVLENEMARANNYEDYGDYWRGDYEVTGAGDYDYSRDQLMKD VERTFAEIKPLYEQLHAYVRAKLMHTYPSYISPTGCLPAHLLGDMWGRFWTNLYSLTVPFEHKPSIDVTEKMENQSWDAERIFKEAEKFFVSISLPYMTQGFWDNSM LTEPGDGRKVVCHPTAWDLGKGDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPYLLRNGANEGFHEAVGEIMSLSAATPHYLKALGLLAPDFHEDNETEIN FLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKQQWMEKWWEMKREIVGVVEPLPHDETYCDPACLFHVAEDYSFIRYYTRTIYQFQFHEALCKTAKHEGALFKC DISNSTEAGQRLLQMLRLGKSEPWTLALENIVGIKTMDVKPLLNYFEPLFTWLKEQNRNSFVGWSTEWTPYSDQSIKVRISLKSALGENAYEWNDNEMYLFQSSVAY AMRKYFSEARNETVLFGEDNVWVSDKKPRISFKFFVTSPNNVSDIIPRTEVENAIRLSRDRINDVFQLDDNSLEFLGIQPTLGPPYEPPVT
SEQ ID NO.54:BtACE2-615(牛)氨基酸序列
STTEEQAKTFLEKFNHEAEDLSYQSSLASWNYNTNITDENVQKMNEARAKWSAFYEEQSRMAKTYSLEEIQNLTLKRQLKALQHSGTSALSAEKSKRLNTILNK MSTIYSTGKVLDPNTQECLALEPGLDDIMENSRDYNRRLWAWEGWRAEVGKQLRPLYEEYVVLENEMARANNYEDYGDYWRGDYEVTGAGDYDYSRDQLMKD VERTFAEIKPLYEQLHAYVRAKLMHTYPSYISPTGCLPAHLLGDMWGRFWTNLYSLTVPFEHKPSIDVTEKMENQSWDAERIFKEAEKFFVSISLPYMTQGFWDNSM LTEPGDGRKVVCHPTAWDLGKGDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPYLLRNGANEGFHEAVGEIMSLSAATPHYLKALGLLAPDFHEDNETEIN FLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKQQWMEKWWEMKREIVGVVEPLPHDETYCDPACLFHVAEDYSFIRYYTRTIYQFQFHEALCKTAKHEGALFKC DISNSTEAGQRLLQMLRLGKSEPWTLALENIVGIKTMDVKPLLNYFEPLFTWLKEQNRNSFVGWSTEWTPYSD
SEQ ID NO.55:DrACE2-740(斑马鱼)氨基酸序列
QTVEDRAREFLNKFDEEASDIMYQYTLASWAYNTDISQENADKEAEAYAIWSEYYNKMSEESNAYPIDQISDPIIKMQLQKLQDKGSGALSPDKASELRNIMSE MSTIYNTATVCKIDDPTDCQTLEPGLESIMAESRDYDERLHVWEGWRVATGMKMRPLYEKYVDLKNEAAKLNNYEDHGDYWRGDYETIDDPKYSYSRDQVIEDA RRIYKEILPLYKELHAYVRAKLQDVYPGHIGSDACLPAHLLGDMWGRFWTNLYPLMIPYPDRPDIDVSSAMVEQGWDEIRLFKEAEKFFMSVNMPAMFDNFWNNS MFIKPEERDVVCHPTAWDMGNRKDFRIKMCTKVNMDDFLTVHHEMGHNQYQMAYRNHPYLLRDGANEGFHEAVGEIMSLSAATPSHLQSLGLLPSDFKQDYETDI NFLLKQALTIVGTLPFTYMLEEWRWQVFKAKIPKDEWMQQWWQMKRELVGVAEAVPRDETYCDPPALFHVSGDYSFIRYFTRTIYQFQFQEALCKAAGHTGPLYK CDITNSTKAGDKLRHMLELGRSMSWTRALEEVAGTTKMDSQPLLHYFSTLMEWLKEENQKNNRVPGWNVNVNPGVLTSSFINDAEISENAFKVRISLKSALGNEAY TWNANDIYLFKSTMAFAMRQYYLKEKNTDVNFTPENIHTYNETARISFKFAVMDPTKTGTVIPKAEVENAIWQERDRINGAFLLSDETLEFVGLMATLAPPKEEKIT SEQ IDNO.56:DrACE2-615(斑马鱼)氨基酸序列
QTVEDRAREFLNKFDEEASDIMYQYTLASWAYNTDISQENADKEAEAYAIWSEYYNKMSEESNAYPIDQISDPIIKMQLQKLQDKGSGALSPDKASELRNIMSE MSTIYNTATVCKIDDPTDCQTLEPGLESIMAESRDYDERLHVWEGWRVATGMKMRPLYEKYVDLKNEAAKLNNYEDHGDYWRGDYETIDDPKYSYSRDQVIEDA RRIYKEILPLYKELHAYVRAKLQDVYPGHIGSDACLPAHLLGDMWGRFWTNLYPLMIPYPDRPDIDVSSAMVEQGWDEIRLFKEAEKFFMSVNMPAMFDNFWNNS MFIKPEERDVVCHPTAWDMGNRKDFRIKMCTKVNMDDFLTVHHEMGHNQYQMAYRNHPYLLRDGANEGFHEAVGEIMSLSAATPSHLQSLGLLPSDFKQDYETDI NFLLKQALTIVGTLPFTYMLEEWRWQVFKAKIPKDEWMQQWWQMKRELVGVAEAVPRDETYCDPPALFHVSGDYSFIRYFTRTIYQFQFQEALCKAAGHTGPLYK CDITNSTKAGDKLRHMLELGRSMSWTRALEEVAGTTKMDSQPLLHYFSTLMEWLKEENQKNNRVPGWNVNVNPGVLTSSFIND
SEQ ID NO.57:dACE2-740(狗)氨基酸序列
STEDLVKTFLEKFNYEAEELSYQSSLASWNYNINITDENVQKMNNAGAKWSAFYEEQSKLAKTYPLEEIQDSTVKRQLRALQHSGSSVLSADKNQRLNTILNSM STVYSTGKACNPSNPQECLLLEPGLDDIMENSKDYNERLWAWEGWRSEVGKQLRPLYEEYVALKNEMARANNYEDYGDYWRGDYEEEWENGYNYSRNQLIDDV ELTFTQIMPLYQHLHAYVRTKLMDTYPSYISPTGCLPAHLLGDMWGRFWTNLYPLTVPFGQKPNIDVTNAMVNQSWDARKIFKEAEKFFVSVGLPNMTQEFWGNS MLTEPSDSRKVVCHPTAWDLGKGDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPNHLKNIGLLPPSFFEDSETEINF LLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKTWWEMKRNIVGVVEPVPHDETYCDPASLFHVANDYSFIRYYTRTIYQFQFQEALCQIAKHEGPLHKCDI SNSSEAGQKLLEMLKLGKSKPWTYALEIVVGAKNMDVRPLLNYFEPLFTWLKEQNRNSFVGWNTDWSPYADQSIKVRISLKSALGEKAYEWNNNEMYLFRSSIAY AMRQYFSEVKNQTIPFVEDNVWVSDLKPRISFNFSVTSPGNVSDIIPRTEVEEAIRMYRSRINDVFRLDDNSLEFLGIQPTPGPPYEPPVT
SEQ ID NO.58:dACE2-615(狗)氨基酸序列
STEDLVKTFLEKFNYEAEELSYQSSLASWNYNINITDENVQKMNNAGAKWSAFYEEQSKLAKTYPLEEIQDSTVKRQLRALQHSGSSVLSADKNQRLNTILNSM STVYSTGKACNPSNPQECLLLEPGLDDIMENSKDYNERLWAWEGWRSEVGKQLRPLYEEYVALKNEMARANNYEDYGDYWRGDYEEEWENGYNYSRNQLIDDV ELTFTQIMPLYQHLHAYVRTKLMDTYPSYISPTGCLPAHLLGDMWGRFWTNLYPLTVPFGQKPNIDVTNAMVNQSWDARKIFKEAEKFFVSVGLPNMTQEFWGNS MLTEPSDSRKVVCHPTAWDLGKGDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPNHLKNIGLLPPSFFEDSETEINF LLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKTWWEMKRNIVGVVEPVPHDETYCDPASLFHVANDYSFIRYYTRTIYQFQFQEALCQIAKHEGPLHKCDI SNSSEAGQKLLEMLKLGKSKPWTYALEIVVGAKNMDVRPLLNYFEPLFTWLKEQNRNSFVGWNTDWSPYAD
SEQ ID NO.59:DcACE2-740(猫)氨基酸序列
STTEELAKTFLEKFNHEAEELSYQSSLASWNYNTNITDENVQKMNEAGAKWSAFYEEQSKLAKTYPLAEIHNTTVKRQLQALQQSGSSVLSADKSQRLNTILNA MSTIYSTGKACNPNNPQECLLLEPGLDDIMENSKDYNERLWAWEGWRAEVGKQLRPLYEEYVALKNEMARANNYEDYGDYWRGDYEEEWTDGYNYSRSQLIKD VEHTFTQIKPLYQHLHAYVRAKLMDTYPSRISPTGCLPAHLLGDMWGRFWTNLYPLTVPFGQKPNIDVTDAMVNQSWDARRIFKEAEKFFVSVGLPNMTQGFWENS MLTEPGDSRKVVCHPTAWDLGKGDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAVQPFLLRNGANEGFHEAVGEIMSLSAATPNHLKTIGLLSPGFSEDSETEIN FLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKEQWMQKWWEMKREIVGVVEPVPHDETYCDPASLFHVANDYSFIRYYTRTIYQFQFQEALCRIAKHEGPLHKCD ISNSSEAGKKLLQMLTLGKSKPWTLALEHVVGEKKMNVTPLLKYFEPLFTWLKEQNRNSFVGWNTDWRPYADQSIKVRISLKSALGDEAYEWNDNEMYLFRSSVA YAMREYFSKVKNQTIPFVEDNVWVSNLKPRISFNFFVTASKNVSDVIPRSEVEEAIRMSRSRINDAFRLDDNSLEFLGIQPTLSPPYQPPVT
SEQ ID NO.60:DcACE2-615(猫)氨基酸序列
STTEELAKTFLEKFNHEAEELSYQSSLASWNYNTNITDENVQKMNEAGAKWSAFYEEQSKLAKTYPLAEIHNTTVKRQLQALQQSGSSVLSADKSQRLNTILNA MSTIYSTGKACNPNNPQECLLLEPGLDDIMENSKDYNERLWAWEGWRAEVGKQLRPLYEEYVALKNEMARANNYEDYGDYWRGDYEEEWTDGYNYSRSQLIKD VEHTFTQIKPLYQHLHAYVRAKLMDTYPSRISPTGCLPAHLLGDMWGRFWTNLYPLTVPFGQKPNIDVTDAMVNQSWDARRIFKEAEKFFVSVGLPNMTQGFWENS MLTEPGDSRKVVCHPTAWDLGKGDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAVQPFLLRNGANEGFHEAVGEIMSLSAATPNHLKTIGLLSPGFSEDSETEIN FLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKEQWMQKWWEMKREIVGVVEPVPHDETYCDPASLFHVANDYSFIRYYTRTIYQFQFQEALCRIAKHEGPLHKCD ISNSSEAGKKLLQMLTLGKSKPWTLALEHVVGEKKMNVTPLLKYFEPLFTWLKEQNRNSFVGWNTDWRPYAD
SEQ ID NO.61:DfACE2-740(雪貂)氨基酸序列
STTEDLAKTFLEKFNYEAEELSYQNSLASWNYNTNITDENIQKMNIAGAKWSAFYEEESQHAKTYPLEEIQDPIIKRQLRALQQSGSSVLSADKRERLNTILNAMS TIYSTGKACNPNNPQECLLLEPGLDDIMENSKDYNERLWAWEGWRSEVGKQLRPLYEEYVALKNEMARANNYEDYGDYWRGDYEEEWADGYSYSRNQLIEDVEH TFTQIKPLYEHLHAYVRAKLMDAYPSRISPTGCLPAHLLGDMWGRFWTNLYPLMVPFRQKPNIDVTDAMVNQSWDARRIFEEAETFFVSVGLPNMTEGFWQNSMLT EPGDNRKVVCHPTAWDLGKRDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAEQPFLLRNGANEGFHEAVGEIMSLSAATPNHLKNIGLLPPDFSEDSETDINFLL KQALTIVGTLPFTYMLEKWRWMVFKGEIPKEQWMQKWWEMKRDIVGVVEPLPHDETYCDPAALFHVANDYSFIRYYTRTIYQFQFQEALCQIAKHEGPLYKCDISN SSEAGQKLHEMLSLGRSKPWTFALERVVGAKTMDVRPLLNYFEPLFTWLKEQNRNSFVGWNTDWSPYADQSIKVRISLKSALGEKAYEWNDNEMYFFQSSIAYAM REYFSKVKNQTIPFVGKDVRVSDLKPRISFNFIVTSPENMSDIIPRADVEEAIRKSRGRINDAFRLDDNSLEFLGIQPTLEPPYQPPVT
SEQ ID NO.62:DfACE2-615(雪貂)氨基酸序列
STTEDLAKTFLEKFNYEAEELSYQNSLASWNYNTNITDENIQKMNIAGAKWSAFYEEESQHAKTYPLEEIQDPIIKRQLRALQQSGSSVLSADKRERLNTILNAMS TIYSTGKACNPNNPQECLLLEPGLDDIMENSKDYNERLWAWEGWRSEVGKQLRPLYEEYVALKNEMARANNYEDYGDYWRGDYEEEWADGYSYSRNQLIEDVEH TFTQIKPLYEHLHAYVRAKLMDAYPSRISPTGCLPAHLLGDMWGRFWTNLYPLMVPFRQKPNIDVTDAMVNQSWDARRIFEEAETFFVSVGLPNMTEGFWQNSMLT EPGDNRKVVCHPTAWDLGKRDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAEQPFLLRNGANEGFHEAVGEIMSLSAATPNHLKNIGLLPPDFSEDSETDINFLL KQALTIVGTLPFTYMLEKWRWMVFKGEIPKEQWMQKWWEMKRDIVGVVEPLPHDETYCDPAALFHVANDYSFIRYYTRTIYQFQFQEALCQIAKHEGPLYKCDISN SSEAGQKLHEMLSLGRSKPWTFALERVVGAKTMDVRPLLNYFEPLFTWLKEQNRNSFVGWNTDWSPYAD
SEQ ID NO.63:MmACE2-740(恒河猴)氨基酸序列
STIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGEKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNT MSTIYSTGKVCNPNNPQECLLLDPGLNEIMEKSLDYNERLWAWEGWRSEVGKQLRPLYEEYVVLKNEMAGANHYKDYGDYWRGDYEVNGVDGYDNNRDQLIED VERTFEEIKPLYEHLHAYVRAKLMNAYPSYISPTGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVNQAWNAQRIFKEAEKFFVSVGLPNMTQGFWEN SMLTDPGNVQKVVCHPTAWDLGKGDFRIIMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEI NFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHK CDISNSTEAGQKLLNMLKLGESEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSS VAYAMRTYFLEIKHQTILFGEEDVRVADLKPRISFNFYVTAPKNVSDIIPRTEVEEAIRISRSRINDAFRLNDNSLEFLGIQTTLAPPYQSPVT
SEQ ID NO.64:MmACE2-615(恒河猴)氨基酸序列
STIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGEKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNT MSTIYSTGKVCNPNNPQECLLLDPGLNEIMEKSLDYNERLWAWEGWRSEVGKQLRPLYEEYVVLKNEMAGANHYKDYGDYWRGDYEVNGVDGYDNNRDQLIED VERTFEEIKPLYEHLHAYVRAKLMNAYPSYISPTGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVNQAWNAQRIFKEAEKFFVSVGLPNMTQGFWEN SMLTDPGNVQKVVCHPTAWDLGKGDFRIIMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEI NFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHK CDISNSTEAGQKLLNMLKLGESEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYAD
SEQ ID NO.65:MjACE2-740(穿山甲)氨基酸序列
STSDEEAKTFLEKFNSEAEELSYQSSLASWNYNTNITDENVQKMNVAGAKWSTFYEEQSKIAKNYQLQNIQNDTIKRQLQALQLSGSSALSADKNQRLNTILNT MSTIYSTGKVCNPGNPQECSLLEPGLDNIMESSKDYNERLWAWEGWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEAEGANGYNYSRDHLIEDV EHIFTQIKPLYEHLHAYVRAKLMDNYPSHISPTGCLPAHLLGDMWGRFWTNLYPLTVPFRQKPNIDVTDAMVNQTWDANRIFKEAEKFFVSVGLPKMTQTFWENSM LTEPGDGRKVVCHPTAWDLGKHDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAMQPYLLRNGANEGFHEAVGEIMSLSAATPKHLKNIGLLPPDFYEDNETEIN FLLKQALTIVGTLPFTYMLEKWRWMVFSGQIPKEQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVANDYSFIRYYTRTIYQFQFQEALCQTAKHEGPLHKC DISNSAEAGQKLLQMLSLGKSKPWTLALERVVGTKNMDVRPLLNYFEPLLTWLKEQNKNSFVGWNTDWSPYAAQSIKVRISLKSALGEKAYEWNDSEMYLFRSSV AYAMREYFSKVKKQTIPFEDECVRVSDLKPRVSFIFFVTLPKNVSAVIPRAEVEEAIRISRSRINDAFRLDDNSLEFLGIQPTLQPPYQPPVT
SEQ ID NO.66:MjACE2-615(穿山甲)氨基酸序列
STSDEEAKTFLEKFNSEAEELSYQSSLASWNYNTNITDENVQKMNVAGAKWSTFYEEQSKIAKNYQLQNIQNDTIKRQLQALQLSGSSALSADKNQRLNTILNT MSTIYSTGKVCNPGNPQECSLLEPGLDNIMESSKDYNERLWAWEGWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEAEGANGYNYSRDHLIEDV EHIFTQIKPLYEHLHAYVRAKLMDNYPSHISPTGCLPAHLLGDMWGRFWTNLYPLTVPFRQKPNIDVTDAMVNQTWDANRIFKEAEKFFVSVGLPKMTQTFWENSM LTEPGDGRKVVCHPTAWDLGKHDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAMQPYLLRNGANEGFHEAVGEIMSLSAATPKHLKNIGLLPPDFYEDNETEIN FLLKQALTIVGTLPFTYMLEKWRWMVFSGQIPKEQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVANDYSFIRYYTRTIYQFQFQEALCQTAKHEGPLHKC DISNSAEAGQKLLQMLSLGKSKPWTLALERVVGTKNMDVRPLLNYFEPLLTWLKEQNKNSFVGWNTDWSPYAA
SEQ ID NO.67:MfACE2-740(土拨鼠)氨基酸序列
STIEELAKTFLDKFNQEAEDLDYQRSLASWNYNTNITKENTQKMNEAEAKWSAFYEKQSKLAKAYPLQEIQNFTLKRQLQALQQSGSSALSANKREQLNTILNT MSTIYSTGKVCNPKKPQECLLLEPGLDGIMANSTDYNERLWVWEGWRSKVGKQLRPLYEEYVVLKNEMARANNYEDYGDYWRGDYEAEGADGYGYNHNQLIED VERTFAEIKPLYEHLHAYVRAKLMNTYPSYISPTGCLPAHLLGDMWGRFWTNLYSLTVPFPEKPNIDVTDAMIKQNWNAVRIFKEAEKFFVSVGLPNMTQGFWENS MLTEPTDGRKVVCHPTAWDLQKGDFRIKMCTKVTMDNFLTAHHEMGHIQYNMAYAIQPYLLRNGANEGFHEAVGEIMSLSATTPKHLKSIGLLPSDFREDNETEINF LLKQALTIVGALPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVMEPVPHDETYCDPAALYHVSNDFSFIRYYTRTIYQFQFQEALCQAAKHEGPLHKC DISNSTEAGQKLLNMLRLGKSKPWTLALENVVGARNMDVRPLLNYFEPLFGWLKDQNRNSFVGWNTNWSPYTDQSIKVRISLKSALGEEAYQWNDNEMYLFRSSV AYAMRMYFSKVKNQTIPFGEEDVWVSDLKPRISFNFFVTTPQNASDIIPRTDVEKAIRMSRGRINGVFRLDDNSLEFLGIQPTLGPPYQPPVT
SEQ ID NO.68:MfACE2-615(土拨鼠)氨基酸序列
STIEELAKTFLDKFNQEAEDLDYQRSLASWNYNTNITKENTQKMNEAEAKWSAFYEKQSKLAKAYPLQEIQNFTLKRQLQALQQSGSSALSANKREQLNTILNT MSTIYSTGKVCNPKKPQECLLLEPGLDGIMANSTDYNERLWVWEGWRSKVGKQLRPLYEEYVVLKNEMARANNYEDYGDYWRGDYEAEGADGYGYNHNQLIED VERTFAEIKPLYEHLHAYVRAKLMNTYPSYISPTGCLPAHLLGDMWGRFWTNLYSLTVPFPEKPNIDVTDAMIKQNWNAVRIFKEAEKFFVSVGLPNMTQGFWENS MLTEPTDGRKVVCHPTAWDLQKGDFRIKMCTKVTMDNFLTAHHEMGHIQYNMAYAIQPYLLRNGANEGFHEAVGEIMSLSATTPKHLKSIGLLPSDFREDNETEINF LLKQALTIVGALPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVMEPVPHDETYCDPAALYHVSNDFSFIRYYTRTIYQFQFQEALCQAAKHEGPLHKC DISNSTEAGQKLLNMLRLGKSKPWTLALENVVGARNMDVRPLLNYFEPLFGWLKDQNRNSFVGWNTNWSPYTD
SEQ ID NO.69:PlACE2-740(果子狸)氨基酸序列
STTEELAKTFLETFNYEAQELSYQSSVASWNYNTNITDENAKNMNEAGAKWSAYYEEQSKLAQTYPLAEIQDAKIKRQLQALQQSGSSVLSADKSQRLN TILNAMSTIYSTGKACNPNNPQECLLLEPGLDNIMENSKDYNERLWAWEGWRAEVGKQLRPLYEEYVALKNEMARANNYEDYGDYWRGDYEEEWTGGYNYSRN QLIQDVEDTFEQIKPLYQHLHAYVRAKLMDTYPSRISRTGCLPAHLLGDMWGRFWTNLYPLTVPFGQKPNIDVTDAMVNQNWDARRIFKEAEKFFVSVGLPNMTQG FWENSMLTEPGDGRKVVCHPTAWDLGKGDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPNHLKTIGLLSPAFSED NETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGAIPKEQWMQKWWEMKRNIVGVVEPVPHDETYCDPASLFHVANDYSFIRYYTRTIYQFQFQEALCQIAKHEGPLHKCDISNSTEAGKKLLEMLSLGRSEPWTLALERVVGAKNMNVTPLLNYFEPLFTWLKEQNRNSFVGWDTDWRPYSDQSIKVRISLKSALGEKAYEWNDNEMYLF RSSIAYAMREYFSKVKNQTIPFVEDNVWVSDLKPRISFNFFVTFSNNVSDVIPRSEVEDAIRMSRSRINDAFRLDDNSLEFLGIEPTLSPPYRPPVT
SEQ ID NO.70:PlACE2-615(果子狸)氨基酸序列
STTEELAKTFLETFNYEAQELSYQSSVASWNYNTNITDENAKNMNEAGAKWSAYYEEQSKLAQTYPLAEIQDAKIKRQLQALQQSGSSVLSADKSQRLNTILNA MSTIYSTGKACNPNNPQECLLLEPGLDNIMENSKDYNERLWAWEGWRAEVGKQLRPLYEEYVALKNEMARANNYEDYGDYWRGDYEEEWTGGYNYSRNQLIQD VEDTFEQIKPLYQHLHAYVRAKLMDTYPSRISRTGCLPAHLLGDMWGRFWTNLYPLTVPFGQKPNIDVTDAMVNQNWDARRIFKEAEKFFVSVGLPNMTQGFWEN SMLTEPGDGRKVVCHPTAWDLGKGDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPNHLKTIGLLSPAFSEDNETEI NFLLKQALTIVGTLPFTYMLEKWRWMVFKGAIPKEQWMQKWWEMKRNIVGVVEPVPHDETYCDPASLFHVANDYSFIRYYTRTIYQFQFQEALCQIAKHEGPLHK CDISNSTEAGKKLLEMLSLGRSEPWTLALERVVGAKNMNVTPLLNYFEPLFTWLKEQNRNSFVGWDTDWRPYSD
SEQ ID NO.71:PsACE2-740(中华鳖)氨基酸序列
DITQEAINFLSEFNVQAEDLSYASSLASWNYNTNITDENAKKMNEAGAKWSVFYDEASTNASKYAIDKITNHTVKLQLQSLQGKGTSVLSGEKYNELNKILSTM STFYSTGTVCKPDNPDICLPLEPGLDAIMASSTDYFERLWAWEGWRADVGKKMRELYERYVELENEAARLNKYSDYGDYWRGNYEVNDPTEYAYSRNQLMEDVE ATFEQIKPLYRELHAYVRYRLEKFYGSDHISSTGCLPAHLLGDMWGRFWTNLYALTVPYPDKPNIDVTSEMVKKNWNATKIFKAAEDFFMSVGLYKMTEGFWKNS MITEPNDGRKVVCHPTAWDMGKKDYRIKMCTKVSMDDFLTVHHEMGHIEYDMAYSNLSYLLRSGANEGFHEAVGEIMSLSAATPKHLKSLDLLEPTFQEDNETDIN FLLKQALTIVGTMPFTYMLEKWRWMVFKGDIPKDEWMKKWWEMKRAIVGVVEPVPHDETYCDPAALFHVANDYSFIRYYTRTIYQFQFQEALCKAANHGGLLHT CDITNSMAAGQKLRDMLALGRSQPWTKALESITGEKKMNATPLLHYFEPLYQWLIKNNSGRAVGWNTFWSPYSGNAIKVRISLKTALGDNAYEWDENELYFFKSSI AYAMRKYFLEVKNQTVSFQCTDIHVWAVTQRVSFYFAVSMPGNATDFIPKSEVETAIRMSRGRINEAFRLDDNTLEFEGLLPTLASPYEPPVT
SEQ ID NO.72:PsACE2-615(中华鳖)氨基酸序列
DITQEAINFLSEFNVQAEDLSYASSLASWNYNTNITDENAKKMNEAGAKWSVFYDEASTNASKYAIDKITNHTVKLQLQSLQGKGTSVLSGEKYNELNKILSTM STFYSTGTVCKPDNPDICLPLEPGLDAIMASSTDYFERLWAWEGWRADVGKKMRELYERYVELENEAARLNKYSDYGDYWRGNYEVNDPTEYAYSRNQLMEDVE ATFEQIKPLYRELHAYVRYRLEKFYGSDHISSTGCLPAHLLGDMWGRFWTNLYALTVPYPDKPNIDVTSEMVKKNWNATKIFKAAEDFFMSVGLYKMTEGFWKNS MITEPNDGRKVVCHPTAWDMGKKDYRIKMCTKVSMDDFLTVHHEMGHIEYDMAYSNLSYLLRSGANEGFHEAVGEIMSLSAATPKHLKSLDLLEPTFQEDNETDIN FLLKQALTIVGTMPFTYMLEKWRWMVFKGDIPKDEWMKKWWEMKRAIVGVVEPVPHDETYCDPAALFHVANDYSFIRYYTRTIYQFQFQEALCKAANHGGLLHT CDITNSMAAGQKLRDMLALGRSQPWTKALESITGEKKMNATPLLHYFEPLYQWLIKNNSGRAVGWNTFWSPYSG
SEQ ID NO.73:RnACE2-740(褐家鼠)氨基酸序列
SLIEEKAESFLNKFNQEAEDLSYQSSLASWNYNTNITEENAQKMNEAAAKWSAFYEEQSKIAQNFSLQEIQNATIKRQLKALQQSGSSALSPDKNKQLNTILNTM STIYSTGKVCNSMNPQECFLLEPGLDEIMATSTDYNRRLWAWEGWRAEVGKQLRPLYEEYVVLKNEMARANNYEDYGDYWRGDYEAEGVEGYNYNRNQLIEDVE NTFKEIKPLYEQLHAYVRTKLMEVYPSYISPTGCLPAHLLGDMWGRFWTNLYPLTTPFLQKPNIDVTDAMVNQSWDAERIFKEAEKFFVSVGLPQMTPGFWTNSML TEPGDDRKVVCHPTAWDLGHGDFRIKMCTKVTMDNFLTAHHEMGHIQYDMAYAKQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLPSNFQEDNETEINFL LKQALTIVGTLPFTYMLEKWRWMVFQDKIPREQWTKKWWEMKREIVGVVEPLPHDETYCDPASLFHVSNDYSFIRYYTRTIYQFQFQEALCQAAKHDGPLHKCDIS NSTEAGQKLLNMLSLGNSGPWTLALENVVGSRNMDVKPLLNYFQPLFVWLKEQNRNSTVGWSTDWSPYADQSIKVRISLKSALGKNAYEWTDNEMYLFRSSVAY AMREYFSREKNQTVPFGEADVWVSDLKPRVSFNFFVTSPKNVSDIIPRSEVEEAIRMSRGRINDIFGLNDNSLEFLGIYPTLKPPYEPPVT
SEQ ID NO.74:RnACE2-615(褐家鼠)氨基酸序列
SLIEEKAESFLNKFNQEAEDLSYQSSLASWNYNTNITEENAQKMNEAAAKWSAFYEEQSKIAQNFSLQEIQNATIKRQLKALQQSGSSALSPDKNKQLNTILNTM STIYSTGKVCNSMNPQECFLLEPGLDEIMATSTDYNRRLWAWEGWRAEVGKQLRPLYEEYVVLKNEMARANNYEDYGDYWRGDYEAEGVEGYNYNRNQLIEDVE NTFKEIKPLYEQLHAYVRTKLMEVYPSYISPTGCLPAHLLGDMWGRFWTNLYPLTTPFLQKPNIDVTDAMVNQSWDAERIFKEAEKFFVSVGLPQMTPGFWTNSML TEPGDDRKVVCHPTAWDLGHGDFRIKMCTKVTMDNFLTAHHEMGHIQYDMAYAKQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLPSNFQEDNETEINFL LKQALTIVGTLPFTYMLEKWRWMVFQDKIPREQWTKKWWEMKREIVGVVEPLPHDETYCDPASLFHVSNDYSFIRYYTRTIYQFQFQEALCQAAKHDGPLHKCDIS NSTEAGQKLLNMLSLGNSGPWTLALENVVGSRNMDVKPLLNYFQPLFVWLKEQNRNSTVGWSTDWSPYAD
SEQ ID NO.75:RfACE2-740(马铁菊头蝠)氨基酸序列
STTEDLAKKFLDDFNSEAENLSHQSSLASWEYNTNISDENVQKMDEAGAKWSDFYEKQSKLAKNFSLEEIHNDTVKLQLQILQQSGSPVLSEDKSKRLNSILNA MSTIYSTGKVCKPNNPQECLLLEPGLDNIMGTSKDYNERLWAWEGWRAEVGKQLRPLYEEYVVLKNEMARGYHYEDYGDYWRRDYETEGSPDLEYSRDQLIKDV ERIFAEIKPLYEQLHAYVRTKLMDTYPFHISPTGCLPAHLLGDMWGRFWTNLYPLTVPFGQKPNIDVTDAMLNQNWDAKRIFKEAEKFFVSIGLPNMTEGFWNNSML TDPGDGRKVVCHPTAWDLGKGDFRIKMCTKVTMEDFLTAHHEMGHIQYDMAYASQPYLLRNGANEGFHEAVGEVMSLSVATPKHLKTMGLLSSDFLEDNETEINF LFKQALNIVGTLPFTYMLEKWRWMVFKGEIPKEEWMKKWWEMKRKIVGVVEPVPHDETYCDPASLFHVANDYSFIRYYTRTIFEFQFHEALCRIAKHDGPLHKCDI SNSTDAGEKLHQMLSVGKSQPWTSVLKDFVGSKNMDVGPLLRYFEPLYTWLTEQNRKSFVGWNTDWSPYADQSIKVRISLKSALGEKAYEWNNNEMYLFRSSVAY AMREYFLKTKNQTILFGEEDVWVSNLKPRISFNFYVTSPRNLSDIIPKPEVEGAIRMSRSRINDAFRLDDNSLEFLGIQPTLGPPYQPPVT
SEQ ID NO.76:RfACE2-615(马铁菊头蝠)氨基酸序列
STTEDLAKKFLDDFNSEAENLSHQSSLASWEYNTNISDENVQKMDEAGAKWSDFYEKQSKLAKNFSLEEIHNDTVKLQLQILQQSGSPVLSEDKSKRLNSILNA MSTIYSTGKVCKPNNPQECLLLEPGLDNIMGTSKDYNERLWAWEGWRAEVGKQLRPLYEEYVVLKNEMARGYHYEDYGDYWRRDYETEGSPDLEYSRDQLIKDV ERIFAEIKPLYEQLHAYVRTKLMDTYPFHISPTGCLPAHLLGDMWGRFWTNLYPLTVPFGQKPNIDVTDAMLNQNWDAKRIFKEAEKFFVSIGLPNMTEGFWNNSML TDPGDGRKVVCHPTAWDLGKGDFRIKMCTKVTMEDFLTAHHEMGHIQYDMAYASQPYLLRNGANEGFHEAVGEVMSLSVATPKHLKTMGLLSSDFLEDNETEINF LFKQALNIVGTLPFTYMLEKWRWMVFKGEIPKEEWMKKWWEMKRKIVGVVEPVPHDETYCDPASLFHVANDYSFIRYYTRTIFEFQFHEALCRIAKHDGPLHKCDI SNSTDAGEKLHQMLSVGKSQPWTSVLKDFVGSKNMDVGPLLRYFEPLYTWLTEQNRKSFVGWNTDWSPYAD
SEQ ID NO.77:sACE2-740(蝾螈)氨基酸序列
DVTNDARVFLDAFNAQAEDLSYENSLASWAYNTNITEENAIKMNEAGAKWTAFYKKANNNASRFPVDQITDPDIKLQILSLGEKGSSVLPDDKYNRLNKALSD MSTIYSTGTVCDNSAKCLQLEPGLDLIMADSTDYHKRLWAWEGWRSEVGKKMRPLYETYVDLNNEAAKLNDYADYGDYWRGNYETQDSGKYAYSRNDLKRDVE RTFKEIQPLYRELHAYVRDKLRGVYGDKYISKNGCLPAHLLGDMWGRFWTNLYPLAVPYPNQPSIDVTSAMNAKKWNVDKMFREAEDFFVSVGLYKMNENFWNF SMLTEPNDGRNVVCHPTAWDMGKNDFRIKMCTKVNMEDFLTVHHEMGHIQYDMAYANLSFLLRNGANEGFHEAVGEIMSLSAATPKHLKSLDLLPPTFVENEETN INFLLRQALTIVATMPFTYMLEEWRWKVFNGEIPRDQWMKKWWQMKREIVGVMEPVPHDETYCDPAALFHVANDYSFIRYYTRTIYQFQFQEALCKAANHNGSLHTCDITNSTLAGQKLRTMLALGNSKPWTMALESITGGKTMDAQPLLHYFDPLYTWLRKNNIDNNRQTYWDTEWSAYTDYEIKVRISLHSAFGDNAYTWDSGEQYLF KSTIAYAMIKYYSEVKSEQVPFTAENVFVTRETLRISFYFHVTDPRNISSFIPKIDVEDAVRLSRGRINSAFNLDDNTLEFVDILSTLSPSVEPPVT
SEQ ID NO.78:sACE2-615(蝾螈)氨基酸序列
DVTNDARVFLDAFNAQAEDLSYENSLASWAYNTNITEENAIKMNEAGAKWTAFYKKANNNASRFPVDQITDPDIKLQILSLGEKGSSVLPDDKYNRLNKALSD MSTIYSTGTVCDNSAKCLQLEPGLDLIMADSTDYHKRLWAWEGWRSEVGKKMRPLYETYVDLNNEAAKLNDYADYGDYWRGNYETQDSGKYAYSRNDLKRDVE RTFKEIQPLYRELHAYVRDKLRGVYGDKYISKNGCLPAHLLGDMWGRFWTNLYPLAVPYPNQPSIDVTSAMNAKKWNVDKMFREAEDFFVSVGLYKMNENFWNF SMLTEPNDGRNVVCHPTAWDMGKNDFRIKMCTKVNMEDFLTVHHEMGHIQYDMAYANLSFLLRNGANEGFHEAVGEIMSLSAATPKHLKSLDLLPPTFVENEETN INFLLRQALTIVATMPFTYMLEEWRWKVFNGEIPRDQWMKKWWQMKREIVGVMEPVPHDETYCDPAALFHVANDYSFIRYYTRTIYQFQFQEALCKAANHNGSLHTCDITNSTLAGQKLRTMLALGNSKPWTMALESITGGKTMDAQPLLHYFDPLYTWLRKNNIDNNRQTYWDTEWSAYTD
SEQ ID NO.79:SsACE2-740(野猪)氨基酸序列
MNKMSSIYSTGTVCKREDPFDCQTLEPGLESVMANMDSDYYERLHVWEGWRVEVGKKMRPLYEDYVDLKNEAAKLNGYEDYGDYWRSNYETIDDSPYNYA RGQLMTDVRHIYKEILPLYKELHAYVRSKLQAKHPEHIHPEGGLPAHLLGDMWGRFWTGLYPISTPFPEKTDIDVTDAMIAQKWPKDRLFQEAEKFFMSVGLYKMF DNFWKDSMLEKPTDGRKVVCHPTAWDMGNREDFRIKMCTEVNMDHFLTAHHEMGHNQYQMAYRNLSYLLRDGANEGFHEAVGEIMSLSAATPKHLKALGLLPD DFVEDKETEINFLMKQALTIVATLPFTYMLEEWRWQVFLGTIPKDQWMQRWWEMKRDMVGVVEPLPRDETYCDPPALFHVSGDYSFIRYFTRTIYQFQFQKALCEA AGHSGPLFKCDITNSTAAGDKLRTMLEFGRSKSWTRALETISGHAKMDSAPLLDYFKDLHVWLIEENRKNNRKPGWRAAEDPFSENAYKVRLSLKAAMGDKAYIWNANEMYLFKANMAYAMRQYYLEVNKTEVLFTTENIHTYKETARISFYFVVTDPANPAVVIPKAEVEAAIRLSRGRINDAFKLDDKTLEFEGLLATLAPPVEQPVT
SEQ ID NO.80:SsACE2-615(野猪)氨基酸序列
STTEELAKTFLEKFNLEAEDLAYQSSLASWNYNTNITDENIQKMNDARAKWSAFYEEQSRIAKTYPLDEIQTLILKRQLQALQQSGTSGLSADKSKRLNTILNTM STIYSSGKVLDPNNPQECLVLEPGLDEIMENSKDYSRRLWAWESWRAEVGKQLRPLYEEYVVLENEMARANNYEDYGDYWRGDYEVTGTGDYDYSRNQLMEDVE RTFAEIKPLYEHLHAYVRAKLMDAYPSRISPTGCLPAHLLGDMWGRFWTNLYPLTVPFGEKPSIDVTEAMVNQSWDAIRIFEEAEKFFVSIGLPNMTQGFWNNSMLT EPGDGRKVVCHPTAWDLGKGDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAIQPYLLRNGANEGFHEAVGEIMSLSAATPHYLKALGLLPPDFYEDSETEINFLL KQALTIVGTLPFTYMLEKWRWMVFKGEIPKEQWMQKWWEMKREIVGVVEPLPHDETYCDPACLFHVAEDYSFIRYYTRTIYQFQFHEALCRTAKHEGPLYKCDISN STEAGQKLLQMLSLGKSEPWTLALENIVGVKTMDVKPLLSYFEPLLTWLKAQNGNSSVGWNTDWTPYAD
SEQ ID NO.81:TeACE2-740(蛇)氨基酸序列
DVTQQAAEFLKQFDARADDLYYAASIASWNYNTNLTEENAKIMHEKDNIFSKFYEEASKNASMYNVNQITNETIRLQLHLLQNVPTNSSTKDQLDTVLRKMST MYSTGTVCKQDDPFNCLPLEPGLDDIMENNWSYSERLWAWEGWRADVGKKMRPLYESYVELKNKYARLRGYADYGDYWRANYEVDLPKEYQYQRAQLITDVE NTLQQIMPLYKHLHAYVRRHLYKHYGPEFINLEGAIPAHLLGDMWGRFWTNLYPLMVPFPNKTSIDVTSAMVTKKWTVNSIFKAAEQFFTSIGLFPMTDNFWNNSM LEEPKDGRKVVCHPTAWDMGKKDYRIKMCTKINMEDFLTAHHEMGHIEYDMAYSDQPFLLRNGANEGFHEAVGEIMSLSAATPKYLKSLGLLEHTFQEDTETDINF LLKQALTIVGTMPFTYMLEKWRWMVFAEQIPKDQWMKKWWEMKREIVGVVEPLPHNEEYCDPAALFHVANDYSFIRYYTRTIYQFQFQEALCQAAGHTGELYKCEISHSTDAGHILKDMLALGSSQPWTKALESITKSQKMDATPFRHYFDPLLKWLEKQNSNENVGWNVNWTPYSKYAIKVRISLKRALGDDAYNWTASEMYLFKSTIA YAMQKYFLEIKNKTVLFQTDNVHVSPVTERISFYFTVSMPTNISELVPKSEVEEAISLSRDRINEAFRLTDQTLEFVGLLPTLAPPYESPIT
SEQ ID NO.82:TeACE2-615(蛇)氨基酸序列
DVTQQAAEFLKQFDARADDLYYAASIASWNYNTNLTEENAKIMHEKDNIFSKFYEEASKNASMYNVNQITNETIRLQLHLLQNVPTNSSTKDQLDTVLRKMST MYSTGTVCKQDDPFNCLPLEPGLDDIMENNWSYSERLWAWEGWRADVGKKMRPLYESYVELKNKYARLRGYADYGDYWRANYEVDLPKEYQYQRAQLITDVE NTLQQIMPLYKHLHAYVRRHLYKHYGPEFINLEGAIPAHLLGDMWGRFWTNLYPLMVPFPNKTSIDVTSAMVTKKWTVNSIFKAAEQFFTSIGLFPMTDNFWNNSM LEEPKDGRKVVCHPTAWDMGKKDYRIKMCTKINMEDFLTAHHEMGHIEYDMAYSDQPFLLRNGANEGFHEAVGEIMSLSAATPKYLKSLGLLEHTFQEDTETDINF LLKQALTIVGTMPFTYMLEKWRWMVFAEQIPKDQWMKKWWEMKREIVGVVEPLPHNEEYCDPAALFHVANDYSFIRYYTRTIYQFQFQEALCQAAGHTGELYKCEISHSTDAGHILKDMLALGSSQPWTKALESITKSQKMDATPFRHYFDPLLKWLEKQNSNENVGWNVNWTPYSK
SEQ ID NO.83:CsACE2-740(银鲑鱼)氨基酸序列
SDLERRAQEFLNQFDGNATHLMYQYSLASWAYNTDISQENLDKLGVQSAIWGEYYSTVSKESEKFPIDQIRDPLIKLQLISLQDKGSGALSADKAAHLNKVMNE MSSIYSTGTVCKREDPFDCQTLEPGLESVMANMDSDYYERLHVWEGWRVEVGKKMRPLYEDYVDLKNEAAKLNDYEDYGDYWRSNYETTDDSPYNYARGQLMT DVRRIYKEILPLYKELHAYVRSKLQAKHPEHIHPEGGLPAHLLGDMWGRFWTGLYPISTPFPEKIDIDVTNAMIAQKWPKDRLFQEAEKFFMSVGLYKMFDNFWKDS MLEKPTDGRKVVCHPTAWDMGNREDFRIKMCTEVNMDHFLTAHHEMGHNQYQMAYRNLSYLLRDGANEGFHEAVGEIMSLSAATPKHLKALGLLPDDFVEDKE TEINFLMKQALTIVATLPFTYMLEEWRWQVFLGTIPKDQWMQRWWEMKRDMVGVVEPLPRDETYCDPPALFHVSGDYSFIRYFTRTIYQFQFQKALCEAAGHSGPL FKCDITNSTAAGDKLRTMLEFGRSKSWTRALETISGNPKMDSAPLLDYFKDLHVWLLEENRKNNRKPGWKAAEDPFSENAYKVRLSLKAAMGDKAYKWNANEMY LFKANMAYAMRQYYLEVNKTAALFTTENIHTYKETARISFYFVVTDPANSAVVIPKAEVEAAIRMSRGRINDAFKLDDKTLEFEGLLATLAPPVEQPVT
SEQ ID NO.84:CsACE2-615(银鲑鱼)氨基酸序列
SDLERRAQEFLNQFDGNATHLMYQYSLASWAYNTDISQENLDKLGVQSAIWGEYYSTVSKESEKFPIDQIRDPLIKLQLISLQDKGSGALSADKAAHLNKVMNE MSSIYSTGTVCKREDPFDCQTLEPGLESVMANMDSDYYERLHVWEGWRVEVGKKMRPLYEDYVDLKNEAAKLNDYEDYGDYWRSNYETTDDSPYNYARGQLMT DVRRIYKEILPLYKELHAYVRSKLQAKHPEHIHPEGGLPAHLLGDMWGRFWTGLYPISTPFPEKIDIDVTNAMIAQKWPKDRLFQEAEKFFMSVGLYKMFDNFWKDS MLEKPTDGRKVVCHPTAWDMGNREDFRIKMCTEVNMDHFLTAHHEMGHNQYQMAYRNLSYLLRDGANEGFHEAVGEIMSLSAATPKHLKALGLLPDDFVEDKE TEINFLMKQALTIVATLPFTYMLEEWRWQVFLGTIPKDQWMQRWWEMKRDMVGVVEPLPRDETYCDPPALFHVSGDYSFIRYFTRTIYQFQFQKALCEAAGHSGPL FKCDITNSTAAGDKLRTMLEFGRSKSWTRALETISGNPKMDSAPLLDYFKDLHVWLLEENRKNNRKPGWKAAEDPFSE
SEQ ID NO.85:RtACE2-740(虹鳟鱼)氨基酸序列
SDLERRAQEFLDQFDGNATHLMYQYSLASWAYNTDISQENLDKLGVQSTIWGEYYSTVSKESEKFPIDQISDPLIRLQLISLQDKGSGALSADKAAHLNKVMNE MSSIYSTGTVCKREDPLDCQTLEPGLESVMANMDSDYYERLHVWEGWRVEVGKKMRPLYEDYVDLKNEAAKLNDYEDYGDYWRSNYETIDDSPYNYARGQLMT DVRRIYKEILPLYKELHAYVRSKLQAKHPEHIHPEGGLPAHLLGDMWGRFWTGLYPISTPFPEKTDIDVTEAMIAQKWPKDRLFQEAEKFFMSVGLYKMFDNFWKDS MLEKPTDGRKVVCHPTAWDMGNREDFRIKMCTEVNMDHFLTAHHEMGHNQYQMAYRNLSYLLRDGANEGFHEAVGEIMSLSAATPKHLKALGLLPGDFVEDKE TEINFLMKQALTIVATLPFTYMLEEWRWQVFLGTIPKDQWMQRWWEMKRDMVGVVEPLPRDETYCDPPALFHVSGDYSFIRYFTRTVYQFQFQKALCEAAGHSGP LFKCDITNSTAAGDKLRTMLEFGRSKSWTRALETISGNAKMDSAPLLDYFKDLHVWLIEENRKNNRKPGWRAAEDPFSANAYKVRLSLKAAMGDKAYMWNANEM YLFKANMAYAMRQYYLEVNKTAALFTTENIHTYKETARISFYFVVTDPANSAVVIPKAEVEAAIRMSRGRINDAFKLDDKTLEFEGLLATLAPPVEQPVT
SEQ ID NO.86:RtACE2-615(虹鳟鱼)氨基酸序列
SDLERRAQEFLDQFDGNATHLMYQYSLASWAYNTDISQENLDKLGVQSTIWGEYYSTVSKESEKFPIDQISDPLIRLQLISLQDKGSGALSADKAAHLNKVMNE MSSIYSTGTVCKREDPLDCQTLEPGLESVMANMDSDYYERLHVWEGWRVEVGKKMRPLYEDYVDLKNEAAKLNDYEDYGDYWRSNYETIDDSPYNYARGQLMT DVRRIYKEILPLYKELHAYVRSKLQAKHPEHIHPEGGLPAHLLGDMWGRFWTGLYPISTPFPEKTDIDVTEAMIAQKWPKDRLFQEAEKFFMSVGLYKMFDNFWKDS MLEKPTDGRKVVCHPTAWDMGNREDFRIKMCTEVNMDHFLTAHHEMGHNQYQMAYRNLSYLLRDGANEGFHEAVGEIMSLSAATPKHLKALGLLPGDFVEDKE TEINFLMKQALTIVATLPFTYMLEEWRWQVFLGTIPKDQWMQRWWEMKRDMVGVVEPLPRDETYCDPPALFHVSGDYSFIRYFTRTVYQFQFQKALCEAAGHSGP LFKCDITNSTAAGDKLRTMLEFGRSKSWTRALETISGNAKMDSAPLLDYFKDLHVWLIEENRKNNRKPGWRAAEDPFSA
SEQ ID NO.87:SalACE2-740(三文鱼)氨基酸序列
MNKMSSIYSTGTVCKREDPFDCQTLEPGLESVMANMDSDYYERLHVWEGWRVEVGKKMRPLYEDYVDLKNEAAKLNGYEDYGDYWRSNYETIDDSP YNYARGQLMTDVRHIYKEILPLYKELHAYVRSKLQAKHPEHIHPEGGLPAHLLGDMWGRFWTGLYPISTPFPEKTDIDVTDAMIAQKWPKDRLFQEAEKFFMSVGL YKMFDNFWKDSMLEKPTDGRKVVCHPTAWDMGNREDFRIKMCTEVNMDHFLTAHHEMGHNQYQMAYRNLSYLLRDGANEGFHEAVGEIMSLSAATPKHLKAL GLLPDDFVEDKETEINFLMKQALTIVATLPFTYMLEEWRWQVFLGTIPKDQWMQRWWEMKRDMVGVVEPLPRDETYCDPPALFHVSGDYSFIRYFTRTIYQFQFQK ALCEAAGHSGPLFKCDITNSTAAGDKLRTMLEFGRSKSWTRALETISGHAKMDSAPLLDYFKDLHVWLIEENRKNNRKPGWRAAEDPFSENAYKVRLSLKAAMGD KAYIWNANEMYLFKANMAYAMRQYYLEVNKTEVLFTTENIHTYKETARISFYFVVTDPANPAVVIPKAEVEAAIRLSRGRINDAFKLDDKTLEFEGLLATLAPPVEQ PVTVWLVVFGVVMGLVVCMGCYLIISGFRDRKKKCAAKAKENAENPYGVTNKTFEREEDEQTG
SEQ ID NO.88:SalACE2-615(三文鱼)氨基酸序列
MNKMSSIYSTGTVCKREDPFDCQTLEPGLESVMANMDSDYYERLHVWEGWRVEVGKKMRPLYEDYVDLKNEAAKLNGYEDYGDYWRSNYETIDDSP YNYARGQLMTDVRHIYKEILPLYKELHAYVRSKLQAKHPEHIHPEGGLPAHLLGDMWGRFWTGLYPISTPFPEKTDIDVTDAMIAQKWPKDRLFQEAEKFFMSVGL YKMFDNFWKDSMLEKPTDGRKVVCHPTAWDMGNREDFRIKMCTEVNMDHFLTAHHEMGHNQYQMAYRNLSYLLRDGANEGFHEAVGEIMSLSAATPKHLKAL GLLPDDFVEDKETEINFLMKQALTIVATLPFTYMLEEWRWQVFLGTIPKDQWMQRWWEMKRDMVGVVEPLPRDETYCDPPALFHVSGDYSFIRYFTRTIYQFQFQK ALCEAAGHSGPLFKCDITNSTAAGDKLRTMLEFGRSKSWTRALETISGHAKMDSAPLLDYFKDLHVWLIEENRKNNRKPGWRAAEDPFSENAYKVRLSLKAAMGD KAYIWNANEMYLFKANMAYAMRQYYLEVNKTEVLFTTENIHTYKETARISFYFVVTDPANPAVVIPKAEVEAAIRLSRGRINDAFKLDDKTLEFEGLLATLAPPVEQ PVT
SEQ ID NO.89:StACE2-740(亚东鲑)氨基酸序列
SDLERRAQEFLDTFDGNATHLMYQYSLASWAYNTDISQENLDKLGVQSAIWGEYYSKVSKESENFPIDQISDPLIKLQLTSLQDKGSGALSADKAAHLNKVMNK MSSIYSTGTVCKREDPFDCQTLEPGLESVMANMDSDYYERLHVWEGWRVEVGKKMRPLYEDYVDLKNEAAKLNGYEDYGDYWRSNYETIDDSPYNYARGQLMT DVRRIYKEILPLYKELHAYVRSKLQAKHPEHIHPEGGLPAHLLGDMWGRFWTGLYPISTPFPEKTDIDVTDAMIAQKWPKDRLFQEAEKFFMSVGLYKMFDNFWKD SMLEKPTDGRKVVCHPTAWDMGNREDFRIKMCTEVNMDHFLTAHHEMGHNQYQMAYRNLSYLLRDGANEGFHEAVGEIMSLSAATPKHLKALGLLPDDFVEDK ETEINFLMKQALTIVATLPFTYMLEEWRWQVFLGTIPKDQWMQRWWEMKRDMVGVVEPLPRDETYCDPPALFHVSGDYSFIRYFTRTIYQFQFQKALCEAAGHSGP LFKCDITNSTAAGDKLRTMLEFGRSKSWTRALETISGHAKMDSAPLLDYFKDLHVWLIEENRKNNRKPGWRAAEDPFSENAYKVRLSLKAAMGDKAYIWNGNEMY LFKANMAYAMRQYYLEVNKTEVLFTTENIHTYKETARISFYFVVTDPANPAVVIPKAEVEAAIRLSRGRINDAFKLDDKTLEFEGLLATLAPPVEQPVT
SEQ ID NO.90:StACE2-615(亚东鲑)氨基酸序列
SDLERRAQEFLDTFDGNATHLMYQYSLASWAYNTDISQENLDKLGVQSAIWGEYYSKVSKESENFPIDQISDPLIKLQLTSLQDKGSGALSADKAAHLNKVMNK MSSIYSTGTVCKREDPFDCQTLEPGLESVMANMDSDYYERLHVWEGWRVEVGKKMRPLYEDYVDLKNEAAKLNGYEDYGDYWRSNYETIDDSPYNYARGQLMT DVRRIYKEILPLYKELHAYVRSKLQAKHPEHIHPEGGLPAHLLGDMWGRFWTGLYPISTPFPEKTDIDVTDAMIAQKWPKDRLFQEAEKFFMSVGLYKMFDNFWKD SMLEKPTDGRKVVCHPTAWDMGNREDFRIKMCTEVNMDHFLTAHHEMGHNQYQMAYRNLSYLLRDGANEGFHEAVGEIMSLSAATPKHLKALGLLPDDFVEDK ETEINFLMKQALTIVATLPFTYMLEEWRWQVFLGTIPKDQWMQRWWEMKRDMVGVVEPLPRDETYCDPPALFHVSGDYSFIRYFTRTIYQFQFQKALCEAAGHSGP LFKCDITNSTAAGDKLRTMLEFGRSKSWTRALETISGHAKMDSAPLLDYFKDLHVWLIEENRKNNRKPGWRAAEDPFSE
SEQ ID NO.91:MlACE2-740(水貂)氨基酸序列
QSTTEDLAKTFLEKFNYEAEELSYQNSLASWNYNTNITDENIQKMNIAGAKWSAFYEEESQHAKTYPLEEIQDPIIKRQLRALQQSGSSVLSADKRERLNTI LNAMSTIYSTGKACNPNNPQECLLLEPGLDDIMENSKDYNERLWAWEGWRSEVGKQLRPLYEEYVALKNEMARANNYEDYGDYWRGDYEEEWADGYSYSRNQL IEDVEHTFTQIKPLYEHLHAYVRAKLMDAYPSRISPTGCLPAHLLGDMWGRFWTNLYPLMVPFGQKPNIDVTDAMVNQSWDARRIFEEAETFFVSVGLPNMTEGFW QNSMLTEPGDNRKVVCHPTAWDLGKRDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAEQPFLLRNGANEGFHEAVGEIMSLSAATPNHLKNIGLLPPDFSEDSET DINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKEQWMQKWWEMKRDIVGVVEPLPHDETYCDPAALFHVANDYSFIRYYTRTIYQFQFQEALCQIAKHEGPLY KCDISNSREAGQKLHEMLSLGRSKPWTFALERVVGAKTMDVRPLLNYFEPLFTWLKEQNRNSFVGWNTDWSPYADQSIKVRISLKSALGEKAYEWNDNEMYFFQS SIAYAMREYFSKVKNQTIPFVGKDVRVSDLKPRISFNFIVTSPENMSDIIPRADVEEAIRKSRGRINDAFRLDDNSLEFLGIQPTLEPPYQPPVT
SEQ ID NO.92:MlACE2-615(水貂)氨基酸序列
QSTTEDLAKTFLEKFNYEAEELSYQNSLASWNYNTNITDENIQKMNIAGAKWSAFYEEESQHAKTYPLEEIQDPIIKRQLRALQQSGSSVLSADKRERLNTI LNAMSTIYSTGKACNPNNPQECLLLEPGLDDIMENSKDYNERLWAWEGWRSEVGKQLRPLYEEYVALKNEMARANNYEDYGDYWRGDYEEEWADGYSYSRNQL IEDVEHTFTQIKPLYEHLHAYVRAKLMDAYPSRISPTGCLPAHLLGDMWGRFWTNLYPLMVPFGQKPNIDVTDAMVNQSWDARRIFEEAETFFVSVGLPNMTEGFW QNSMLTEPGDNRKVVCHPTAWDLGKRDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAEQPFLLRNGANEGFHEAVGEIMSLSAATPNHLKNIGLLPPDFSEDSET DINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKEQWMQKWWEMKRDIVGVVEPLPHDETYCDPAALFHVANDYSFIRYYTRTIYQFQFQEALCQIAKHEGPLY KCDISNSREAGQKLHEMLSLGRSKPWTFALERVVGAKTMDVRPLLNYFEPLFTWLKEQNRNSFVGWNTDWSPYAD
SEQ ID NO.93:VvACE2-740(狐狸)氨基酸序列
QSTEDLVNTFLEKFNYEAEELSYQSSLASWDYNTNISDENVQKMNNAGAKWSAFYEEQSKLAKTYPLEEIQDSTVKRQLRALQHSGSSVLSADKNQRLN TILNSMSTIYSTGKACNPSNPQECLLLEPGLDDIMENSKDYNERLWAWEGWRSEVGKQLRPLYEEYVALKNEMARANNYEDYGDYWRGDYEEEWENGYNYSRNQ LIDDVEHTFTQIMPLYQHLHAYVRTKLMDTYPSYISPTGCLPAHLLGDMWGRFWTNLYPLTVPFGQKPNIDVTNAMVNQSWDARKIFKEAEKFFVSVGLPNMTQGF WENSMLTEPSDSRKVVCHPTAWDLGKGDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPNHLKNIGLLPPSFFEDSE TEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKTWWEMKRNIVGVVEPVPHDETYCDPASLFHVANDYSFIRYYTRTIYQFQFQEALCQIAKHEGPLHKCDISNSSEAGQKLLEMLKLGKSKPWTYALEIVVGAKNMDVRPLLNYFEPLFTWLKEQNRNSFVGWNTDWSPYADQSIKVRISLKSALGEKAYEWNNNEMYLFR SSIAYAMRRYFSEVKKQTIPFVEDNVWVSDLKPRISFNFFVTSPGNVSDIIPRTEVEKAIRMYRGRINDVFRLDDNSLEFLGIQPTLGPSYEPPVTI
SEQ ID NO.94:VvACE2-615(狐狸)氨基酸序列
QSTEDLVNTFLEKFNYEAEELSYQSSLASWDYNTNISDENVQKMNNAGAKWSAFYEEQSKLAKTYPLEEIQDSTVKRQLRALQHSGSSVLSADKNQRLN TILNSMSTIYSTGKACNPSNPQECLLLEPGLDDIMENSKDYNERLWAWEGWRSEVGKQLRPLYEEYVALKNEMARANNYEDYGDYWRGDYEEEWENGYNYSRNQ LIDDVEHTFTQIMPLYQHLHAYVRTKLMDTYPSYISPTGCLPAHLLGDMWGRFWTNLYPLTVPFGQKPNIDVTNAMVNQSWDARKIFKEAEKFFVSVGLPNMTQGF WENSMLTEPSDSRKVVCHPTAWDLGKGDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPNHLKNIGLLPPSFFEDSE TEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKTWWEMKRNIVGVVEPVPHDETYCDPASLFHVANDYSFIRYYTRTIYQFQFQEALCQIAKHEGPLHKCDISNSSEAGQKLLEMLKLGKSKPWTYALEIVVGAKNMDVRPLLNYFEPLFTWLKEQNRNSFVGWNTDWSPYAD
SEQ ID NO.95:EcACE2-740(马)氨基酸序列
QSTTEDLAKTFLEKFNSEAEELSHQSSLASWSYNTNITDENVQKMNEAGARWSAFYEEQCKLAKTYPLEEIQNLTVKRQLQALQQSGSSVLSADKSKRLN EILNTMSTIYSTGKVCNPSNPQECLLLEPGLDAIMENSKDYNQRLWAWEGWRSEVGKQLRPLYEEYVVLKNEMARANNYEDYGDYWRGDYEAEGPSGYDYSRDQ LIEDVERTFAEIKPLYEHLHAYVRAKLMDTYPSHINPTGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQSWDAKRIFEEAEKFFVSVGLPNMTQGF WENSMLTEPGDGRKVVCHPTAWDLGKGDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAVQPYLLRNGANEGFHEAVGEIMSLSAATPNHLKAIGLLPPDFYED SETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKEEWMKKWWEMKREIVGVVEPVPHDETYCDPAALFHVANDYSFIRYYTRTIYQFQFQEALCQTAKHEGPLHKCDISNSTEAGQKLLQMLSLGKSEPWTLALERIVGVKNMDVRPLLNYFEPLFTWLKDQNKNSFVGWSTNWSPYADQSIKVRISLKSALGEKSYEWNDNEMYLF QSSVAYAMRVYFLKAKNQTILFGEEDVWVSDLKPRISFNFFVTSPKNASDIIPRTDVEEAIRMSRSRINDAFRLDDNTLEFLGIQPTLGPPYQPPVTV
SEQ ID NO.96:EcACE2-615(马)氨基酸序列
QSTTEDLAKTFLEKFNSEAEELSHQSSLASWSYNTNITDENVQKMNEAGARWSAFYEEQCKLAKTYPLEEIQNLTVKRQLQALQQSGSSVLSADKSKRLN EILNTMSTIYSTGKVCNPSNPQECLLLEPGLDAIMENSKDYNQRLWAWEGWRSEVGKQLRPLYEEYVVLKNEMARANNYEDYGDYWRGDYEAEGPSGYDYSRDQ LIEDVERTFAEIKPLYEHLHAYVRAKLMDTYPSHINPTGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQSWDAKRIFEEAEKFFVSVGLPNMTQGF WENSMLTEPGDGRKVVCHPTAWDLGKGDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAVQPYLLRNGANEGFHEAVGEIMSLSAATPNHLKAIGLLPPDFYED SETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKEEWMKKWWEMKREIVGVVEPVPHDETYCDPAALFHVANDYSFIRYYTRTIYQFQFQEALCQTAKHEGPLHKCDISNSTEAGQKLLQMLSLGKSEPWTLALERIVGVKNMDVRPLLNYFEPLFTWLKDQNKNSFVGWSTNWSPYAD
SEQ ID NO.97:RBD-StrepII的核酸序列 CGCGTACAACCGACAGAGTCAATTGTACGTTTTCCTAACATCACCAATCTCTGTCCGTTTGGTGAAGTCTTTAACGCTACGCGGTTTGCTTCCGTTTACGCGTGG AACAGGAAACGAATATCGAACTGCGTAGCTGATTACTCCGTGTTATATAATAGTGCGAGCTTCTCTACTTTCAAGTGTTATGGTGTTTCACCAACAAAGTTAAA TGACCTCTGCTTTACCAACGTATACGCCGATAGTTTTGTCATAAGAGGCGACGAGGTGAGGCAAATTGCGCCTGGACAGACAGGGAAAATAGCAGATTACAA TTACAAATTGCCTGACGATTTCACCGGCTGTGTTATCGCATGGAACTCTAATAATCTAGATTCTAAGGTCGGAGGCAATTACAATTATCTTTACCGTCTGTTTCG GAAGTCCAACTTGAAGCCGTTCGAACGCGACATCTCGACGGAGATTTATCAAGCCGGCAGCACTCCATGTAACGGGGTTGAGGGGTTCAACTGCTATTTCCCC CTCCAGTCGTATGGGTTCCAGCCAACGAATGGAGTCGGTTATCAACCCTATAGAGTGGTGGTACTGTCATTTGAACTATTACACGCCCCTGCAACAGTTTGCGG TCCCAAGAAAAGTACTAACTTGGTCAAAAATAAACTTCCGGAAACCGGATGGAGTCACCCTCAGTTCGAGAAA
SEQ ID NO.98:RBD-StrepII的氨基酸序列 RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPD DFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLV KNKLPETGWSHPQFEK
序列表
<110> 清华大学
清华大学深圳国际研究生院
<120> 使用真核细胞发酵表达血管紧张素转化酶2的方法
<130> PE01428
<141> 2021-11-24
<150> 2020113628565
<151> 2020-11-27
<160> 98
<170> SIPOSequenceListing 1.0
<210> 1
<211> 2169
<212> DNA
<213> Artificial Sequence
<220>
<223> hACE2-740（人）核苷酸序列
<400> 1
cagtccacta ttgaggagca ggcgaagaca ttccttgaca agttcaatca cgaagcagaa 60
gatttattct accagtcatc acttgcatca tggaactata acacaaacat cacagaagag 120
aacgtacaga atatgaacaa cgcaggagat aaatggtcag catttcttaa agaacaatca 180
acacttgcac aaatgtatcc tcttcaagaa atccagaatt taacagttaa acttcaactt 240
caagcacttc aacagaatgg ttcatcagtt ctttcagaag ataaatcaaa gcggttgaac 300
acaatcctta acacaatgtc aacaatctac tctaccggga aagtctgcaa ccctgataac 360
cctcaagaat gtcttcttct tgaacctgga cttaacgaaa tcatggcaaa ctcacttgat 420
tataacgaaa gactttgggc atgggaatca tggagatcag aagttggaaa gcagctcaga 480
ccactctacg aggagtacgt tgttcttaag aatgagatgg caagagcaaa ccattatgaa 540
gattatggag attattggag aggagattat gaagttaacg gagttgatgg atatgattat 600
tcaagaggtc agctaattga ggacgttgaa catacatttg aagaaatcaa accgttgtac 660
gagcacctgc atgcatatgt tagagcaaag ctcatgaacg catatccttc atatatctca 720
cctatcggat gtcttcctgc acatcttctt ggagatatgt ggggccgttt ctggactaac 780
ctttattcac ttacagttcc tttcgggcag aaaccaaata tcgatgttac agatgcaatg 840
gttgatcaag catgggatgc acaaagaatc tttaaagaag cagagaagtt cttcgtatct 900
gttggacttc ctaacatgac acaaggattc tgggagaact ccatgcttac agatcctgga 960
aatgtccaga aggcagtttg tcatcctaca gcatgggatc ttggaaaggg tgatttccgt 1020
attcttatgt gtacaaaggt cactatggat gatttcctca ctgcacatca tgaaatggga 1080
catatccaat atgatatggc atatgcagca caacctttct tattaagaaa cggagcaaac 1140
gaaggatttc atgaagcagt tggagaaatc atgtcacttt cagcagcaac acctaaacat 1200
cttaaatcaa tcggacttct ttcacctgat ttccaggagg ataacgaaac agaaatcaac 1260
tttcttctta aacaagcact tacaatcgtt ggaacacttc ctttcactta catgcttgag 1320
aagtggcgct ggatggtgtt caagggtgaa atccctaaag atcaatggat gaagaagtgg 1380
tgggaaatga agagggagat cgttggagtt gttgaacctg ttcctcatga tgaaacatat 1440
tgtgacccag cctctctgtt ccacgtgtct aatgactaca gtttcatacg ctactacacg 1500
cgcaccctat atcaatttca atttcaagaa gcactttgtc aagcagcaaa gcacgaggga 1560
cctcttcata aatgtgatat ctcaaactca acagaagcag gacagaagct gtttaatatg 1620
cttagacttg gaaagagcga gccttggaca cttgcacttg agaatgtagt tggagcaaag 1680
aatatgaacg ttagacctct tcttaactat ttcgagccat tgttcacttg gcttaaagat 1740
cagaataaga actcctttgt tggatggtca acagattggt caccttatgc agatcaatca 1800
atcaaagtta gaatctcact taaatcagca cttggagata aagcatatga atggaacgat 1860
aacgaaatgt acctatttcg aagttccgtc gcttacgcta tgcgtcagta ctttctgaaa 1920
gtgaagaatc aaatgatcct gttcggcgag gaagatgtta gagttgcaaa ccttaaacct 1980
agaatctcat ttaacttctt cgtcaccgca cctaagaatg tctcagatat catccctaga 2040
acagaagttg agaaggctat tagaatgtca agatcaagaa tcaacgatgc atttagactt 2100
aacgataact cacttgaatt tcttggaatc caacctacac ttggacctcc taaccaacct 2160
cctgtttca 2169
<210> 2
<211> 1794
<212> DNA
<213> Artificial Sequence
<220>
<223> hACE2-615（人）核苷酸序列
<400> 2
cagtccacta ttgaggagca ggcgaagaca ttccttgaca agttcaatca cgaagcagaa 60
gatttattct accagtcatc acttgcatca tggaactata acacaaacat cacagaagag 120
aacgtacaga atatgaacaa cgcaggagat aaatggtcag catttcttaa agaacaatca 180
acacttgcac aaatgtatcc tcttcaagaa atccagaatt taacagttaa acttcaactt 240
caagcacttc aacagaatgg ttcatcagtt ctttcagaag ataaatcaaa gcggttgaac 300
acaatcctta acacaatgtc aacaatctac tctaccggga aagtctgcaa ccctgataac 360
cctcaagaat gtcttcttct tgaacctgga cttaacgaaa tcatggcaaa ctcacttgat 420
tataacgaaa gactttgggc atgggaatca tggagatcag aagttggaaa gcagctcaga 480
ccactctacg aggagtacgt tgttcttaag aatgagatgg caagagcaaa ccattatgaa 540
gattatggag attattggag aggagattat gaagttaacg gagttgatgg atatgattat 600
tcaagaggtc agctaattga ggacgttgaa catacatttg aagaaatcaa accgttgtac 660
gagcacctgc atgcatatgt tagagcaaag ctcatgaacg catatccttc atatatctca 720
cctatcggat gtcttcctgc acatcttctt ggagatatgt ggggccgttt ctggactaac 780
ctttattcac ttacagttcc tttcgggcag aaaccaaata tcgatgttac agatgcaatg 840
gttgatcaag catgggatgc acaaagaatc tttaaagaag cagagaagtt cttcgtatct 900
gttggacttc ctaacatgac acaaggattc tgggagaact ccatgcttac agatcctgga 960
aatgtccaga aggcagtttg tcatcctaca gcatgggatc ttggaaaggg tgatttccgt 1020
attcttatgt gtacaaaggt cactatggat gatttcctca ctgcacatca tgaaatggga 1080
catatccaat atgatatggc atatgcagca caacctttct tattaagaaa cggagcaaac 1140
gaaggatttc atgaagcagt tggagaaatc atgtcacttt cagcagcaac acctaaacat 1200
cttaaatcaa tcggacttct ttcacctgat ttccaggagg ataacgaaac agaaatcaac 1260
tttcttctta aacaagcact tacaatcgtt ggaacacttc ctttcactta catgcttgag 1320
aagtggcgct ggatggtgtt caagggtgaa atccctaaag atcaatggat gaagaagtgg 1380
tgggaaatga agagggagat cgttggagtt gttgaacctg ttcctcatga tgaaacatat 1440
tgtgacccag cctctctgtt ccacgtgtct aatgactaca gtttcatacg ctactacacg 1500
cgcaccctat atcaatttca atttcaagaa gcactttgtc aagcagcaaa gcacgaggga 1560
cctcttcata aatgtgatat ctcaaactca acagaagcag gacagaagct gtttaatatg 1620
cttagacttg gaaagagcga gccttggaca cttgcacttg agaatgtagt tggagcaaag 1680
aatatgaacg ttagacctct tcttaactat ttcgagccat tgttcacttg gcttaaagat 1740
cagaataaga actcctttgt tggatggtca acagattggt caccttatgc agat 1794
<210> 3
<211> 2166
<212> DNA
<213> Artificial Sequence
<220>
<223> AtACE2-740（老虎）核苷酸序列
<400> 3
tccactactg aggaattggc taagactttt ttggagaagt ttaaccacga ggccgaggag 60
ttgtcttacc aatcttcttt ggcttcttgg aactacaaca ctaacattac cgatgagaac 120
gtccagaaga tgaacgaagc tggtgctaag tggtctgctt tttacgaaga acaatctaag 180
ttggccgaaa cctacccatt ggctgaaatt cataacacca ctgttaagcg tcagttgcag 240
gctttgcaac aatctggttc ttctgttttg tctgccgata agtctcaaag attgaacact 300
atcttgaacg ccatgtccac tatctactct actggtaagg cctgtaaccc aaacaaccca 360
caagaatgtt tgttgttgga accaggtttg gatgatatta tggagaactc caaggactac 420
aacgaacgtt tgtgggcttg ggaaggttgg agagctgaag ttggtaagca attgagacca 480
ttgtacgaag aatacgtcgc tttgaagaac gagatggcca gagctaacaa ctacgaagat 540
tacggtgatt actggagagg tgattacgaa gaagaatgga ctgatggtta caactactct 600
cgttctcaat tgatcaagga cgtcgaacat accttcaccc agatcaagcc attgtaccaa 660
cacttgcatg cttacgttag agctaagttg atggattctt acccctctag aatttcccca 720
actggttgtt tgccagctca tttgttgggt gatatgtggg gtagattttg gactaacttg 780
tacccattga ctgtcccatt tggtcaaaag ccaaacattg acgttactga cgctatggtt 840
aaccagtcct gggatgctag aagaattttc aaggaggctg aaaagttttt cgtctccgtt 900
ggtttgccaa acatgactca aggtttttgg gaaaactcta tgttgaccga accaggtaac 960
tctcaaaagg ttgtttgtca tccaactgcc tgggatttgg gtaagggtga ttttagaatt 1020
aagatgtgca ccaaggtcac catggacgac tttttgaccg cccaccatga aatgggtcat 1080
attcaatacg atatggccta cgccgttcag ccatttttgt tgagaaacgg tgctaacgaa 1140
ggttttcatg aagctgttgg tgaaattatg tccttgtccg ctgctactcc aaaccatttg 1200
aagactattg gtttgttgcc accaggtttt tctgaagatt ctgaaactga aatcaacttc 1260
ttgttgaagc aggccttgac tatcgtcggt accttgccat ttacctacat gttggagaag 1320
tggagatgga tggtttttaa gggtgaaatt ccaaaggagc agtggatgca aaagtggtgg 1380
gaaatgaaga gagaaattgt cggtgttgtc gagccagttc cacatgatga aacttactgt 1440
gatccagctt ctttgtttca cgtcgctaac gattactctt tcatcagata ctacacccgc 1500
accatttacc agttccagtt tcaggaagct ttgtgcagaa ttgccaagca cgaaggtcca 1560
ttgcataagt gtgatatttc taactcctcc gaggccggta agaagttgtt gcaaatgttg 1620
actttgggca agtccaagcc atggactttg gctttggaac atgttgttgg tgaaaagaac 1680
atgaacgtca ccccattgtt gaagtacttc gaaccattgt ttacctggtt gaaggagcaa 1740
aacagaaact ctttcgtcgg ttggaacact gattggagac catacgctga tcaatccatc 1800
aaggtcagaa tttccttgaa gtctgccttg ggtgataagg cttacgaatg gaacgataac 1860
gaaatgtact tgttccgttc ctctgttgct tacgccatga gagaatactt ttctaaggtt 1920
aagaaccaga ccatcccatt cgttgaggat aacgtctggg tctctaactt gaagccaaga 1980
atttctttta acttcttcgt caccgcctcc aagaacgttt ctgatgttat tccacgtcgt 2040
gaggtcgaag aagccattag aatgtctcgt tctagaatta acgacgcctt ccgtttggat 2100
gacaactcct tggaattttt gggtattcag ccaactttgt ccccaccata ccaaccacca 2160
gttact 2166
<210> 4
<211> 1791
<212> DNA
<213> Artificial Sequence
<220>
<223> AtACE2-615（老虎）核苷酸序列
<400> 4
tccactactg aggaattggc taagactttt ttggagaagt ttaaccacga ggccgaggag 60
ttgtcttacc aatcttcttt ggcttcttgg aactacaaca ctaacattac cgatgagaac 120
gtccagaaga tgaacgaagc tggtgctaag tggtctgctt tttacgaaga acaatctaag 180
ttggccgaaa cctacccatt ggctgaaatt cataacacca ctgttaagcg tcagttgcag 240
gctttgcaac aatctggttc ttctgttttg tctgccgata agtctcaaag attgaacact 300
atcttgaacg ccatgtccac tatctactct actggtaagg cctgtaaccc aaacaaccca 360
caagaatgtt tgttgttgga accaggtttg gatgatatta tggagaactc caaggactac 420
aacgaacgtt tgtgggcttg ggaaggttgg agagctgaag ttggtaagca attgagacca 480
ttgtacgaag aatacgtcgc tttgaagaac gagatggcca gagctaacaa ctacgaagat 540
tacggtgatt actggagagg tgattacgaa gaagaatgga ctgatggtta caactactct 600
cgttctcaat tgatcaagga cgtcgaacat accttcaccc agatcaagcc attgtaccaa 660
cacttgcatg cttacgttag agctaagttg atggattctt acccctctag aatttcccca 720
actggttgtt tgccagctca tttgttgggt gatatgtggg gtagattttg gactaacttg 780
tacccattga ctgtcccatt tggtcaaaag ccaaacattg acgttactga cgctatggtt 840
aaccagtcct gggatgctag aagaattttc aaggaggctg aaaagttttt cgtctccgtt 900
ggtttgccaa acatgactca aggtttttgg gaaaactcta tgttgaccga accaggtaac 960
tctcaaaagg ttgtttgtca tccaactgcc tgggatttgg gtaagggtga ttttagaatt 1020
aagatgtgca ccaaggtcac catggacgac tttttgaccg cccaccatga aatgggtcat 1080
attcaatacg atatggccta cgccgttcag ccatttttgt tgagaaacgg tgctaacgaa 1140
ggttttcatg aagctgttgg tgaaattatg tccttgtccg ctgctactcc aaaccatttg 1200
aagactattg gtttgttgcc accaggtttt tctgaagatt ctgaaactga aatcaacttc 1260
ttgttgaagc aggccttgac tatcgtcggt accttgccat ttacctacat gttggagaag 1320
tggagatgga tggtttttaa gggtgaaatt ccaaaggagc agtggatgca aaagtggtgg 1380
gaaatgaaga gagaaattgt cggtgttgtc gagccagttc cacatgatga aacttactgt 1440
gatccagctt ctttgtttca cgtcgctaac gattactctt tcatcagata ctacacccgc 1500
accatttacc agttccagtt tcaggaagct ttgtgcagaa ttgccaagca cgaaggtcca 1560
ttgcataagt gtgatatttc taactcctcc gaggccggta agaagttgtt gcaaatgttg 1620
actttgggca agtccaagcc atggactttg gctttggaac atgttgttgg tgaaaagaac 1680
atgaacgtca ccccattgtt gaagtacttc gaaccattgt ttacctggtt gaaggagcaa 1740
aacagaaact ctttcgtcgg ttggaacact gattggagac catacgctga t 1791
<210> 5
<211> 2163
<212> DNA
<213> Artificial Sequence
<220>
<223> BtACE2-740（牛）核苷酸序列
<400> 5
tccactactg aagaacaagc taagactttc ttggagaagt ttaaccacga ggccgaagat 60
ttgtcttacc aatcttcttt ggcttcctgg aactacaaca ctaacattac cgacgagaac 120
gtccaaaaga tgaacgaagc cagagctaag tggtctgctt tttacgaaga acaatctcgt 180
atggccaaga cttactcctt ggaagagatt cagaacttga ctttgaagcg tcaattgaag 240
gctttgcagc actctggtac ttctgctttg tctgctgaaa agtctaagag attgaacacc 300
attttgaaca agatgtccac catctactcc accggtaagg ttttggaccc aaacactcaa 360
gaatgtttgg ctttggaacc aggtttggat gatattatgg aaaactcccg tgactacaac 420
cgtcgtttgt gggcttggga aggttggaga gctgaagttg gtaagcaatt gagaccattg 480
tacgaagaat acgtcgtttt ggaaaacgag atggccagag ctaacaacta cgaagattac 540
ggtgattact ggagaggtga ttacgaagtt actggtgctg gtgattacga ttactctaga 600
gatcaattga tgaaggacgt cgaaagaact ttcgccgaaa ttaagccatt gtacgagcaa 660
ttgcatgcct acgttagagc taagttgatg catacttacc catcttacat ttcccccacc 720
ggttgtttgc cagctcattt gttgggtgat atgtggggta gattttggac taacttgtac 780
tctttgaccg tcccatttga gcataagcca tctattgatg tcactgagaa gatggaaaac 840
cagtcttggg atgctgaaag aatttttaag gaggccgaaa agttcttcgt ctccatttcc 900
ttgccataca tgacccaagg tttttgggat aactctatgt tgactgagcc aggtgatggt 960
agaaaggttg tttgtcatcc aactgcttgg gatttgggta agggtgattt tagaattaag 1020
atgtgcacca aggtcaccat ggacgacttc ttgactgctc atcatgaaat gggtcatatc 1080
caatacgata tggcctacgc tgctcaacca tacttgttga gaaacggtgc taacgaaggt 1140
tttcatgaag ctgttggtga aattatgtct ttgtccgctg ctactccaca ttacttgaag 1200
gctttgggtt tgttggctcc agattttcat gaagataacg agaccgaaat taacttcttg 1260
ttgaagcagg ccttgaccat cgttggtact ttgccattta cttacatgtt ggagaagtgg 1320
agatggatgg tttttaaggg tgaaattcca aagcaacagt ggatggaaaa gtggtgggaa 1380
atgaagagag aaattgtcgg tgttgtcgag ccattgccac atgatgaaac ttactgtgat 1440
ccagcttgtt tgtttcacgt tgctgaagat tactccttta tcagatacta cacccgtacc 1500
atctaccagt tccaatttca tgaggccttg tgcaagactg ctaagcatga aggtgctttg 1560
tttaagtgtg atatctccaa ctccactgag gccggtcaaa gattgttgca aatgttgaga 1620
ttgggtaagt ccgaaccatg gactttggct ttggaaaaca ttgttggtat taagaccatg 1680
gacgtcaagc cattgttgaa ctactttgag ccattgttta cttggttgaa ggagcagaac 1740
cgtaactctt ttgtcggttg gtctactgaa tggactccat actctgatca atccatcaag 1800
gtcagaatct ctttgaagtc cgctttgggt gagaacgctt acgaatggaa cgataacgaa 1860
atgtacttgt tccagtcctc cgttgcttac gctatgagaa agtacttttc cgaagctcgt 1920
aacgaaactg ttttgttcgg tgaagataac gtctgggttt ctgataagaa gccaagaatt 1980
tctttcaagt tcttcgtcac ctctcccaac aacgtttctg atatcattcc acgtaccgag 2040
gttgaaaacg ctattagatt gtctcgtgat cgtatcaacg atgtctttca attggatgac 2100
aactccttgg agtttttggg tattcaacca actttgggtc caccatacga accaccagtt 2160
act 2163
<210> 6
<211> 1788
<212> DNA
<213> Artificial Sequence
<220>
<223> BtACE2-615（牛）核苷酸序列
<400> 6
tccactactg aagaacaagc taagactttc ttggagaagt ttaaccacga ggccgaagat 60
ttgtcttacc aatcttcttt ggcttcctgg aactacaaca ctaacattac cgacgagaac 120
gtccaaaaga tgaacgaagc cagagctaag tggtctgctt tttacgaaga acaatctcgt 180
atggccaaga cttactcctt ggaagagatt cagaacttga ctttgaagcg tcaattgaag 240
gctttgcagc actctggtac ttctgctttg tctgctgaaa agtctaagag attgaacacc 300
attttgaaca agatgtccac catctactcc accggtaagg ttttggaccc aaacactcaa 360
gaatgtttgg ctttggaacc aggtttggat gatattatgg aaaactcccg tgactacaac 420
cgtcgtttgt gggcttggga aggttggaga gctgaagttg gtaagcaatt gagaccattg 480
tacgaagaat acgtcgtttt ggaaaacgag atggccagag ctaacaacta cgaagattac 540
ggtgattact ggagaggtga ttacgaagtt actggtgctg gtgattacga ttactctaga 600
gatcaattga tgaaggacgt cgaaagaact ttcgccgaaa ttaagccatt gtacgagcaa 660
ttgcatgcct acgttagagc taagttgatg catacttacc catcttacat ttcccccacc 720
ggttgtttgc cagctcattt gttgggtgat atgtggggta gattttggac taacttgtac 780
tctttgaccg tcccatttga gcataagcca tctattgatg tcactgagaa gatggaaaac 840
cagtcttggg atgctgaaag aatttttaag gaggccgaaa agttcttcgt ctccatttcc 900
ttgccataca tgacccaagg tttttgggat aactctatgt tgactgagcc aggtgatggt 960
agaaaggttg tttgtcatcc aactgcttgg gatttgggta agggtgattt tagaattaag 1020
atgtgcacca aggtcaccat ggacgacttc ttgactgctc atcatgaaat gggtcatatc 1080
caatacgata tggcctacgc tgctcaacca tacttgttga gaaacggtgc taacgaaggt 1140
tttcatgaag ctgttggtga aattatgtct ttgtccgctg ctactccaca ttacttgaag 1200
gctttgggtt tgttggctcc agattttcat gaagataacg agaccgaaat taacttcttg 1260
ttgaagcagg ccttgaccat cgttggtact ttgccattta cttacatgtt ggagaagtgg 1320
agatggatgg tttttaaggg tgaaattcca aagcaacagt ggatggaaaa gtggtgggaa 1380
atgaagagag aaattgtcgg tgttgtcgag ccattgccac atgatgaaac ttactgtgat 1440
ccagcttgtt tgtttcacgt tgctgaagat tactccttta tcagatacta cacccgtacc 1500
atctaccagt tccaatttca tgaggccttg tgcaagactg ctaagcatga aggtgctttg 1560
tttaagtgtg atatctccaa ctccactgag gccggtcaaa gattgttgca aatgttgaga 1620
ttgggtaagt ccgaaccatg gactttggct ttggaaaaca ttgttggtat taagaccatg 1680
gacgtcaagc cattgttgaa ctactttgag ccattgttta cttggttgaa ggagcagaac 1740
cgtaactctt ttgtcggttg gtctactgaa tggactccat actctgat 1788
<210> 7
<211> 2208
<212> DNA
<213> Artificial Sequence
<220>
<223> DrACE2-740（斑马鱼）核苷酸序列
<400> 7
caaactgttg aagatcgtgc tcgtgaattt ttgaacaagt ttgatgagga agcttccgac 60
attatgtacc agtacacctt ggcttcttgg gcttacaaca ctgatatttc tcaagagaac 120
gccgacaagg aagctgaagc ttacgctatt tggtctgaat actacaacaa gatgtccgag 180
gaatctaacg cttacccaat tgatcaaatt tccgacccaa tcatcaagat gcagttgcaa 240
aagttgcagg acaagggttc tggtgctttg tctccagata aggcttctga attgagaaac 300
attatgtccg agatgtctac catttacaac accgctaccg tttgcaagat tgacgatcca 360
actgattgtc agactttgga accaggtttg gaatctatta tggccgaatc tagagactac 420
gacgaacgtt tgcatgtttg ggaaggttgg agagttgcta ctggtatgaa gatgagacca 480
ttgtacgaaa agtacgtcga tttgaagaac gaggctgcta agttgaacaa ctacgaagat 540
catggtgatt actggagagg tgattacgaa actattgacg atccaaagta ctcttactcc 600
cgtgaccaag ttattgagga tgctagaaga atttacaagg agatattgcc cttgtacaag 660
gagttgcacg cttacgttag agctaagttg caagatgttt acccaggtca tattggttct 720
gatgcttgtt tgccagctca tttgttgggt gatatgtggg gtagattttg gactaacttg 780
tacccattga tgatcccata cccagataga ccagatattg acgtctcttc cgctatggtt 840
gagcaaggtt gggatgaaat tagattgttt aaggaggccg agaagttttt catgtctgtt 900
aacatgccag ccatgttcga caacttttgg aacaactcta tgttcatcaa gccagaggaa 960
cgtgacgttg tttgtcatcc aactgcttgg gatatgggta acagaaagga ttttagaatc 1020
aagatgtgca ccaaggtcaa catggacgat ttcttgactg tccaccatga gatgggtcat 1080
aaccaatacc agatggctta cagaaaccat ccatacttgt tgagagatgg tgctaacgaa 1140
ggttttcatg aagctgttgg tgaaattatg tctttgtccg ccgctactcc atctcatttg 1200
caatctttgg gtttgttgcc atctgatttt aagcaggatt acgaaaccga tatcaacttc 1260
ttgttgaagc aggctttgac tatcgttggt actttgccat ttacttacat gttggaggaa 1320
tggcgttggc aggtttttaa ggctaagatt ccaaaggacg agtggatgca acaatggtgg 1380
caaatgaaga gagaattggt tggtgttgct gaagctgttc caagagatga aacttactgt 1440
gatccaccag ctttgtttca tgtttctggt gattactctt tcatccgtta cttcaccaga 1500
accatttacc agttccaatt tcaggaagcc ttgtgcaagg ctgccggtca tactggtcca 1560
ttgtacaagt gtgatattac caactccacc aaggctggtg ataagttgag acatatgttg 1620
gaattgggta gatccatgtc ctggactaga gctttggaag aagttgctgg tactactaag 1680
atggattctc aaccattgtt gcactacttt tccaccttga tggagtggtt gaaggaagag 1740
aaccaaaaga acaacagagt tcccggttgg aacgttaacg ttaacccagg tgttttgact 1800
tcttctttta tcaacgacgc cgaaatttcc gaaaacgcct tcaaggtcag aatttctttg 1860
aagtctgctt tgggtaacga ggcctacact tggaacgcta acgatattta cttgtttaag 1920
tccaccatgg cctttgccat gagacaatac tacttgaagg agaagaacac cgatgttaac 1980
tttaccccag agaacatcca tacttacaac gaaactgcta gaatctcctt caagttcgcc 2040
gttatggacc caactaagac tggtactgtt attccaaagg ctgaagttga aaacgccatt 2100
tggcaagaaa gagatagaat taacggtgcc tttttgttgt ccgacgaaac tttggaattt 2160
gtcggtttga tggctacctt ggctccacca aaggaagaaa agattact 2208
<210> 8
<211> 1842
<212> DNA
<213> Artificial Sequence
<220>
<223> DrACE2-615（斑马鱼）核苷酸序列
<400> 8
caaactgttg aagatcgtgc tcgtgaattt ttgaacaagt ttgatgagga agcttccgac 60
attatgtacc agtacacctt ggcttcttgg gcttacaaca ctgatatttc tcaagagaac 120
gccgacaagg aagctgaagc ttacgctatt tggtctgaat actacaacaa gatgtccgag 180
gaatctaacg cttacccaat tgatcaaatt tccgacccaa tcatcaagat gcagttgcaa 240
aagttgcagg acaagggttc tggtgctttg tctccagata aggcttctga attgagaaac 300
attatgtccg agatgtctac catttacaac accgctaccg tttgcaagat tgacgatcca 360
actgattgtc agactttgga accaggtttg gaatctatta tggccgaatc tagagactac 420
gacgaacgtt tgcatgtttg ggaaggttgg agagttgcta ctggtatgaa gatgagacca 480
ttgtacgaaa agtacgtcga tttgaagaac gaggctgcta agttgaacaa ctacgaagat 540
catggtgatt actggagagg tgattacgaa actattgacg atccaaagta ctcttactcc 600
cgtgaccaag ttattgagga tgctagaaga atttacaagg agatattgcc cttgtacaag 660
gagttgcacg cttacgttag agctaagttg caagatgttt acccaggtca tattggttct 720
gatgcttgtt tgccagctca tttgttgggt gatatgtggg gtagattttg gactaacttg 780
tacccattga tgatcccata cccagataga ccagatattg acgtctcttc cgctatggtt 840
gagcaaggtt gggatgaaat tagattgttt aaggaggccg agaagttttt catgtctgtt 900
aacatgccag ccatgttcga caacttttgg aacaactcta tgttcatcaa gccagaggaa 960
cgtgacgttg tttgtcatcc aactgcttgg gatatgggta acagaaagga ttttagaatc 1020
aagatgtgca ccaaggtcaa catggacgat ttcttgactg tccaccatga gatgggtcat 1080
aaccaatacc agatggctta cagaaaccat ccatacttgt tgagagatgg tgctaacgaa 1140
ggttttcatg aagctgttgg tgaaattatg tctttgtccg ccgctactcc atctcatttg 1200
caatctttgg gtttgttgcc atctgatttt aagcaggatt acgaaaccga tatcaacttc 1260
ttgttgaagc aggctttgac tatcgttggt actttgccat ttacttacat gttggaggaa 1320
tggcgttggc aggtttttaa ggctaagatt ccaaaggacg agtggatgca acaatggtgg 1380
caaatgaaga gagaattggt tggtgttgct gaagctgttc caagagatga aacttactgt 1440
gatccaccag ctttgtttca tgtttctggt gattactctt tcatccgtta cttcaccaga 1500
accatttacc agttccaatt tcaggaagcc ttgtgcaagg ctgccggtca tactggtcca 1560
ttgtacaagt gtgatattac caactccacc aaggctggtg ataagttgag acatatgttg 1620
gaattgggta gatccatgtc ctggactaga gctttggaag aagttgctgg tactactaag 1680
atggattctc aaccattgtt gcactacttt tccaccttga tggagtggtt gaaggaagag 1740
aaccaaaaga acaacagagt tcccggttgg aacgttaacg ttaacccagg tgttttgact 1800
tcttctttta tcaacgacgc cgaaatttcc gaacaccatc ac 1842
<210> 9
<211> 2163
<212> DNA
<213> Artificial Sequence
<220>
<223> dACE2-740（狗）核苷酸序列
<400> 9
tccaccgaag atttggttaa gacctttttg gaaaagttca actacgaggc cgaagagttg 60
tcttaccagt cttctttggc ttcttggaac tacaacatta acatcaccga cgaaaacgtc 120
caaaagatga acaacgccgg tgctaagtgg tctgcttttt acgaagaaca atctaagttg 180
gccaagacct acccattgga agaaattcaa gattccaccg tcaagcgtca gttgcgtgct 240
ttgcaacatt ctggttcttc tgttttgtct gccgataaga accaacgttt gaacactatt 300
ttgaactcca tgtccaccgt ttactctacc ggtaaggcct gtaacccatc taacccacaa 360
gaatgtttgt tgttggaacc aggtttggat gatattatgg agaactctaa ggactacaac 420
gagcgtttgt gggcttggga aggttggcgt tctgaagttg gtaagcaatt gagaccattg 480
tacgaagaat acgtcgcttt gaagaacgag atggctagag ctaacaacta cgaagattac 540
ggtgattact ggagaggtga ttacgaagaa gaatgggaaa acggttacaa ctactctaga 600
aaccaattga tcgacgatgt cgaattgacc tttacccaga tcatgccatt gtaccaacat 660
ttgcacgctt acgttagaac taagttgatg gatacttacc catcctacat ctccccaact 720
ggttgtttgc cagctcattt gttgggtgat atgtggggta gattttggac taacttgtac 780
ccattgactg tcccatttgg tcaaaagcca aacattgacg tcaccaacgc tatggttaac 840
caatcttggg atgctagaaa gattttcaag gaggccgaga agttcttcgt ctctgtcggt 900
ttgccaaaca tgactcaaga attttggggt aactctatgt tgaccgaacc atctgattct 960
agaaaggttg tttgtcaccc aactgcttgg gatttgggta agggtgattt tagaattaag 1020
atgtgcacca aggtcaccat ggacgatttt ttgactgctc accacgagat gggtcatatt 1080
caatacgata tggcttacgc cgctcaacca tttttgttga gaaacggtgc taacgaaggt 1140
tttcatgaag ctgttggtga aattatgtcc ttgtctgctg ctactccaaa ccatttgaag 1200
aacattggtt tgttgccacc atcttttttc gaggactctg aaactgaaat taacttcttg 1260
ttgaagcagg ccttgaccat tgtcggtact ttgccattta cctacatgtt ggaaaagtgg 1320
agatggatgg tttttaaggg tgaaattccc aaggaccagt ggatgaagac ttggtgggaa 1380
atgaagagaa acattgtcgg tgttgtcgaa ccagttccac atgatgaaac ttactgtgat 1440
ccagcttctt tgtttcacgt tgctaacgat tactccttta tccgttacta cactcgtact 1500
atctaccagt tccaattcca ggaggccttg tgccagatcg ccaagcatga aggtccattg 1560
cataagtgtg atatttccaa ctcctctgag gccggtcaaa agttgttgga aatgttgaag 1620
ttgggtaagt ctaagccatg gacttacgct ttggaaattg ttgttggtgc taagaacatg 1680
gacgtcagac cattgttgaa ctacttcgaa ccattgttta cctggttgaa ggagcagaac 1740
agaaactcct ttgtcggttg gaacactgat tggtctccat acgctgatca atccattaag 1800
gttcgtatct ccttgaagtc tgccttgggt gaaaaggctt acgaatggaa caacaacgaa 1860
atgtacttgt tccgttcttc catcgcctac gccatgcgtc aatacttttc tgaagttaag 1920
aaccagacca tccccttcgt tgaagacaac gtttgggttt ctgatttgaa gccaagaatt 1980
tccttcaact tctccgtcac ctccccaggt aacgtctctg atattattcc aagaactgag 2040
gtcgaagagg ctatcagaat gtaccgttct agaatcaacg acgtcttcag attggatgac 2100
aactccttgg aatttttggg catccaacca actccaggtc caccatacga accaccagtt 2160
act 2163
<210> 10
<211> 1788
<212> DNA
<213> Artificial Sequence
<220>
<223> dACE2-615（狗）核苷酸序列
<400> 10
tccaccgaag atttggttaa gacctttttg gaaaagttca actacgaggc cgaagagttg 60
tcttaccagt cttctttggc ttcttggaac tacaacatta acatcaccga cgaaaacgtc 120
caaaagatga acaacgccgg tgctaagtgg tctgcttttt acgaagaaca atctaagttg 180
gccaagacct acccattgga agaaattcaa gattccaccg tcaagcgtca gttgcgtgct 240
ttgcaacatt ctggttcttc tgttttgtct gccgataaga accaacgttt gaacactatt 300
ttgaactcca tgtccaccgt ttactctacc ggtaaggcct gtaacccatc taacccacaa 360
gaatgtttgt tgttggaacc aggtttggat gatattatgg agaactctaa ggactacaac 420
gagcgtttgt gggcttggga aggttggcgt tctgaagttg gtaagcaatt gagaccattg 480
tacgaagaat acgtcgcttt gaagaacgag atggctagag ctaacaacta cgaagattac 540
ggtgattact ggagaggtga ttacgaagaa gaatgggaaa acggttacaa ctactctaga 600
aaccaattga tcgacgatgt cgaattgacc tttacccaga tcatgccatt gtaccaacat 660
ttgcacgctt acgttagaac taagttgatg gatacttacc catcctacat ctccccaact 720
ggttgtttgc cagctcattt gttgggtgat atgtggggta gattttggac taacttgtac 780
ccattgactg tcccatttgg tcaaaagcca aacattgacg tcaccaacgc tatggttaac 840
caatcttggg atgctagaaa gattttcaag gaggccgaga agttcttcgt ctctgtcggt 900
ttgccaaaca tgactcaaga attttggggt aactctatgt tgaccgaacc atctgattct 960
agaaaggttg tttgtcaccc aactgcttgg gatttgggta agggtgattt tagaattaag 1020
atgtgcacca aggtcaccat ggacgatttt ttgactgctc accacgagat gggtcatatt 1080
caatacgata tggcttacgc cgctcaacca tttttgttga gaaacggtgc taacgaaggt 1140
tttcatgaag ctgttggtga aattatgtcc ttgtctgctg ctactccaaa ccatttgaag 1200
aacattggtt tgttgccacc atcttttttc gaggactctg aaactgaaat taacttcttg 1260
ttgaagcagg ccttgaccat tgtcggtact ttgccattta cctacatgtt ggaaaagtgg 1320
agatggatgg tttttaaggg tgaaattccc aaggaccagt ggatgaagac ttggtgggaa 1380
atgaagagaa acattgtcgg tgttgtcgaa ccagttccac atgatgaaac ttactgtgat 1440
ccagcttctt tgtttcacgt tgctaacgat tactccttta tccgttacta cactcgtact 1500
atctaccagt tccaattcca ggaggccttg tgccagatcg ccaagcatga aggtccattg 1560
cataagtgtg atatttccaa ctcctctgag gccggtcaaa agttgttgga aatgttgaag 1620
ttgggtaagt ctaagccatg gacttacgct ttggaaattg ttgttggtgc taagaacatg 1680
gacgtcagac cattgttgaa ctacttcgaa ccattgttta cctggttgaa ggagcagaac 1740
agaaactcct ttgtcggttg gaacactgat tggtctccat acgctgat 1788
<210> 11
<211> 2166
<212> DNA
<213> Artificial Sequence
<220>
<223> DcACE2-740（猫）核苷酸序列
<400> 11
tccactactg aggaattggc taagactttt ttggagaagt ttaaccacga ggccgaagag 60
ttgtcctacc aatcttcttt ggcttcttgg aactacaaca ctaacattac cgacgagaac 120
gtccagaaga tgaacgaagc tggtgctaag tggtctgctt tttacgaaga acaatctaag 180
ttggccaaga cctacccatt ggctgaaatt cataacacca ctgtcaagag acaattgcag 240
gctttgcaac aatctggttc ttctgttttg tctgctgata agtctcaacg tttgaacact 300
atcttgaacg ccatgtctac tatttactcc actggtaagg cttgtaaccc aaacaaccca 360
caagaatgtt tgttgttgga accaggtttg gatgatatta tggagaactc taaggactac 420
aacgagcgtt tgtgggcttg ggaaggttgg agagctgaag ttggtaagca attgagacca 480
ttgtacgaag aatacgttgc cttgaagaac gaaatggcta gagctaacaa ctacgaagat 540
tacggtgatt actggagagg tgattacgaa gaagaatgga ctgatggtta caactactct 600
cgttctcaat tgatcaagga cgtcgaacac accttcactc aaatcaagcc attgtaccaa 660
catttgcacg cctacgttag agccaagttg atggatactt acccatctag aatttccccc 720
accggttgtt tgccagctca tttgttgggt gatatgtggg gtagattttg gactaacttg 780
tacccattga ctgtcccatt tggtcaaaag ccaaacattg atgttaccga cgccatggtt 840
aaccaatctt gggatgctag aagaattttc aaggaggccg aaaagttctt tgtttccgtt 900
ggtttgccaa acatgactca gggtttttgg gaaaactcta tgttgactga gccaggtgat 960
tctagaaagg ttgtttgtca tccaactgcc tgggatttgg gtaagggtga ttttagaatt 1020
aagatgtgca ccaaggtcac catggacgat tttttgactg cccatcatga gatgggtcac 1080
attcaatacg atatggctta cgctgttcaa ccatttttgt tgagaaacgg tgctaacgaa 1140
ggttttcatg aagctgttgg tgaaattatg tccttgtctg ccgctactcc aaaccatttg 1200
aagactattg gtttgttgtc cccaggtttt tccgaagact ctgaaactga aattaacttc 1260
ttgttgaagc aggccttgac catcgttggt accttgccat ttacctacat gttggaaaag 1320
tggagatgga tggtttttaa gggtgaaatt cccaaggaac aatggatgca aaagtggtgg 1380
gaaatgaaga gagaaattgt cggtgttgtc gagccagttc cacatgatga aacttactgt 1440
gatccagctt ctttgtttca cgtcgctaac gattactctt ttatcagata ctacacccgt 1500
accatctacc agtttcagtt tcaggaggct ttgtgtagaa tcgctaagca tgaaggtcca 1560
ttgcataagt gtgatatttc caactcttcc gaggccggta agaagttgtt gcaaatgttg 1620
actttgggta agtctaagcc atggactttg gctttggaac atgttgttgg tgaaaagaag 1680
atgaacgtca ccccattgtt gaagtacttt gagccattgt ttacctggtt gaaggagcaa 1740
aacagaaact cttttgtcgg ctggaacacc gattggagac catacgctga tcagtctatc 1800
aaggtcagaa tttccttgaa gtccgccttg ggtgacgaag cttacgaatg gaacgataac 1860
gaaatgtact tgttccgttc ttccgttgcc tacgctatgc gtgagtactt ttctaaggtt 1920
aagaaccaga ctattccatt cgtcgaggat aacgtctggg tttctaactt gaagccaaga 1980
atttctttca acttcttcgt caccgcttcc aagaacgttt ccgatgttat tccaagatcc 2040
gaagttgaag aagccattcg tatgtctaga tccagaatca acgacgcttt tagattggac 2100
gacaactcct tggaattttt gggtattcaa ccaactttgt ccccaccata ccaaccacca 2160
gttact 2166
<210> 12
<211> 1791
<212> DNA
<213> Artificial Sequence
<220>
<223> DcACE2-615（猫）核苷酸序列
<400> 12
tccactactg aggaattggc taagactttt ttggagaagt ttaaccacga ggccgaagag 60
ttgtcctacc aatcttcttt ggcttcttgg aactacaaca ctaacattac cgacgagaac 120
gtccagaaga tgaacgaagc tggtgctaag tggtctgctt tttacgaaga acaatctaag 180
ttggccaaga cctacccatt ggctgaaatt cataacacca ctgtcaagag acaattgcag 240
gctttgcaac aatctggttc ttctgttttg tctgctgata agtctcaacg tttgaacact 300
atcttgaacg ccatgtctac tatttactcc actggtaagg cttgtaaccc aaacaaccca 360
caagaatgtt tgttgttgga accaggtttg gatgatatta tggagaactc taaggactac 420
aacgagcgtt tgtgggcttg ggaaggttgg agagctgaag ttggtaagca attgagacca 480
ttgtacgaag aatacgttgc cttgaagaac gaaatggcta gagctaacaa ctacgaagat 540
tacggtgatt actggagagg tgattacgaa gaagaatgga ctgatggtta caactactct 600
cgttctcaat tgatcaagga cgtcgaacac accttcactc aaatcaagcc attgtaccaa 660
catttgcacg cctacgttag agccaagttg atggatactt acccatctag aatttccccc 720
accggttgtt tgccagctca tttgttgggt gatatgtggg gtagattttg gactaacttg 780
tacccattga ctgtcccatt tggtcaaaag ccaaacattg atgttaccga cgccatggtt 840
aaccaatctt gggatgctag aagaattttc aaggaggccg aaaagttctt tgtttccgtt 900
ggtttgccaa acatgactca gggtttttgg gaaaactcta tgttgactga gccaggtgat 960
tctagaaagg ttgtttgtca tccaactgcc tgggatttgg gtaagggtga ttttagaatt 1020
aagatgtgca ccaaggtcac catggacgat tttttgactg cccatcatga gatgggtcac 1080
attcaatacg atatggctta cgctgttcaa ccatttttgt tgagaaacgg tgctaacgaa 1140
ggttttcatg aagctgttgg tgaaattatg tccttgtctg ccgctactcc aaaccatttg 1200
aagactattg gtttgttgtc cccaggtttt tccgaagact ctgaaactga aattaacttc 1260
ttgttgaagc aggccttgac catcgttggt accttgccat ttacctacat gttggaaaag 1320
tggagatgga tggtttttaa gggtgaaatt cccaaggaac aatggatgca aaagtggtgg 1380
gaaatgaaga gagaaattgt cggtgttgtc gagccagttc cacatgatga aacttactgt 1440
gatccagctt ctttgtttca cgtcgctaac gattactctt ttatcagata ctacacccgt 1500
accatctacc agtttcagtt tcaggaggct ttgtgtagaa tcgctaagca tgaaggtcca 1560
ttgcataagt gtgatatttc caactcttcc gaggccggta agaagttgtt gcaaatgttg 1620
actttgggta agtctaagcc atggactttg gctttggaac atgttgttgg tgaaaagaag 1680
atgaacgtca ccccattgtt gaagtacttt gagccattgt ttacctggtt gaaggagcaa 1740
aacagaaact cttttgtcgg ctggaacacc gattggagac catacgctga t 1791
<210> 13
<211> 2166
<212> DNA
<213> Artificial Sequence
<220>
<223> DfACE2-740（雪貂）核苷酸序列
<400> 13
tctactaccg aagatttggc taagactttc ttggaaaagt tcaactacga ggccgaagaa 60
ttgtcttacc aaaactcttt ggcttcctgg aactacaaca ctaacattac tgatgagaac 120
atccagaaga tgaacatcgc cggtgccaag tggtctgctt tttacgaaga agaatctcag 180
catgccaaga cctacccatt ggaagaaatt caggacccaa ttattaagcg tcagttgaga 240
gccttgcaac agtctggttc ttctgttttg tctgctgata agagagaacg cttgaacact 300
attttgaacg ccatgtccac tatctactcc actggtaagg cttgtaaccc aaacaaccca 360
caagaatgtt tgttgttgga accaggtttg gatgatatta tggaaaactc caaggactac 420
aacgagcgtt tgtgggcttg ggaaggttgg cgttctgaag ttggtaagca attgagacca 480
ttgtacgaag aatacgtcgc tttgaagaac gaaatggcca gagctaacaa ctacgaagat 540
tacggtgatt actggagagg tgattacgaa gaagaatggg ctgatggtta ctcttactct 600
agaaaccaat tgatcgagga cgtcgagcat acttttactc aaatcaagcc attgtacgag 660
cacttgcacg cttacgttag agctaagttg atggatgctt acccatctag aatttcccca 720
actggttgtt tgccagctca tttgttgggt gatatgtggg gtagattttg gactaacttg 780
tacccattga tggtcccatt tagacagaag ccaaacattg acgttactga cgctatggtt 840
aaccaatctt gggatgctag aagaattttc gaggaggctg aaaccttttt tgtttccgtt 900
ggtttgccaa acatgaccga aggtttttgg caaaactcta tgttgactga gccaggtgat 960
aacagaaagg ttgtttgtca tccaactgcc tgggatttgg gtaagagaga ttttagaatt 1020
aagatgtgca ccaaggtcac catggacgac ttcttgactg ctcatcatga aatgggtcat 1080
attcaatacg acatggccta cgctgaacaa ccatttttgt tgagaaacgg tgctaacgaa 1140
ggttttcatg aagctgttgg tgaaattatg tccttgtctg ccgctactcc aaaccatttg 1200
aagaacattg gtttgttgcc cccagatttt tccgaagact ctgaaactga cattaacttc 1260
ttgttgaagc aagccttgac catcgttggt actttgccat ttacttacat gttggagaag 1320
tggcgttgga tggtttttaa gggtgaaatt ccaaaggagc agtggatgca aaagtggtgg 1380
gaaatgaaga gagatattgt cggtgttgtt gagccattgc cacatgatga aacttactgt 1440
gatccagctg ctttgtttca tgttgctaac gattactctt tcatccgtta ctacacccgt 1500
actatctacc agtttcaatt tcaggaagcc ttgtgtcaaa ttgccaagca cgaaggtcca 1560
ttgtacaagt gtgatatttc taactcctcc gaggccggtc aaaagttgca tgaaatgttg 1620
tctttgggtc gttctaagcc atggactttt gctttggaaa gagttgttgg tgctaagact 1680
atggatgtta gaccattgtt gaactacttc gagccattgt ttacttggtt gaaggagcag 1740
aacagaaact ccttcgtcgg ttggaacact gattggtctc catacgctga tcaatccatt 1800
aaggtccgta tctctttgaa gtctgctttg ggtgaaaagg cttacgaatg gaacgataac 1860
gaaatgtact ttttccagtc ctccatcgct tacgctatga gagaatactt ttccaaggtc 1920
aagaaccaga ctattccatt tgttggtaag gacgttagag tctccgattt gaagccaaga 1980
atttccttta acttcatcgt cacctcccca gagaacatgt ctgatattat tccaagagcc 2040
gatgtcgaag aggccattcg taagtctaga ggtagaatta acgatgcctt tcgtttggac 2100
gataactcct tggaattttt gggtatccag ccaaccttgg agccaccata ccaaccacca 2160
gttact 2166
<210> 14
<211> 1791
<212> DNA
<213> Artificial Sequence
<220>
<223> : DfACE2-615（雪貂）核苷酸序列
<400> 14
tctactaccg aagatttggc taagactttc ttggaaaagt tcaactacga ggccgaagaa 60
ttgtcttacc aaaactcttt ggcttcctgg aactacaaca ctaacattac tgatgagaac 120
atccagaaga tgaacatcgc cggtgccaag tggtctgctt tttacgaaga agaatctcag 180
catgccaaga cctacccatt ggaagaaatt caggacccaa ttattaagcg tcagttgaga 240
gccttgcaac agtctggttc ttctgttttg tctgctgata agagagaacg cttgaacact 300
attttgaacg ccatgtccac tatctactcc actggtaagg cttgtaaccc aaacaaccca 360
caagaatgtt tgttgttgga accaggtttg gatgatatta tggaaaactc caaggactac 420
aacgagcgtt tgtgggcttg ggaaggttgg cgttctgaag ttggtaagca attgagacca 480
ttgtacgaag aatacgtcgc tttgaagaac gaaatggcca gagctaacaa ctacgaagat 540
tacggtgatt actggagagg tgattacgaa gaagaatggg ctgatggtta ctcttactct 600
agaaaccaat tgatcgagga cgtcgagcat acttttactc aaatcaagcc attgtacgag 660
cacttgcacg cttacgttag agctaagttg atggatgctt acccatctag aatttcccca 720
actggttgtt tgccagctca tttgttgggt gatatgtggg gtagattttg gactaacttg 780
tacccattga tggtcccatt tagacagaag ccaaacattg acgttactga cgctatggtt 840
aaccaatctt gggatgctag aagaattttc gaggaggctg aaaccttttt tgtttccgtt 900
ggtttgccaa acatgaccga aggtttttgg caaaactcta tgttgactga gccaggtgat 960
aacagaaagg ttgtttgtca tccaactgcc tgggatttgg gtaagagaga ttttagaatt 1020
aagatgtgca ccaaggtcac catggacgac ttcttgactg ctcatcatga aatgggtcat 1080
attcaatacg acatggccta cgctgaacaa ccatttttgt tgagaaacgg tgctaacgaa 1140
ggttttcatg aagctgttgg tgaaattatg tccttgtctg ccgctactcc aaaccatttg 1200
aagaacattg gtttgttgcc cccagatttt tccgaagact ctgaaactga cattaacttc 1260
ttgttgaagc aagccttgac catcgttggt actttgccat ttacttacat gttggagaag 1320
tggcgttgga tggtttttaa gggtgaaatt ccaaaggagc agtggatgca aaagtggtgg 1380
gaaatgaaga gagatattgt cggtgttgtt gagccattgc cacatgatga aacttactgt 1440
gatccagctg ctttgtttca tgttgctaac gattactctt tcatccgtta ctacacccgt 1500
actatctacc agtttcaatt tcaggaagcc ttgtgtcaaa ttgccaagca cgaaggtcca 1560
ttgtacaagt gtgatatttc taactcctcc gaggccggtc aaaagttgca tgaaatgttg 1620
tctttgggtc gttctaagcc atggactttt gctttggaaa gagttgttgg tgctaagact 1680
atggatgtta gaccattgtt gaactacttc gagccattgt ttacttggtt gaaggagcag 1740
aacagaaact ccttcgtcgg ttggaacact gattggtctc catacgctga t 1791
<210> 15
<211> 2166
<212> DNA
<213> Artificial Sequence
<220>
<223> MmACE2-740（恒河猴）核苷酸序列
<400> 15
tctaccattg aagaacaggc taagactttc ttggataagt ttaaccacga agccgaggat 60
ttgttttacc agtcctcttt ggcttcctgg aactacaaca ctaacattac tgaagagaac 120
gtccagaaca tgaacaacgc tggtgaaaag tggtctgctt ttttgaagga acaatccacc 180
ttggcccaaa tgtacccatt gcaagaaatt caaaacttga ctgtcaagtt gcagttgcag 240
gctttgcaac aaaacggttc ttctgttttg tctgaggata agtctaagcg tttgaacacc 300
attttgaaca ctatgtctac catctactcc accggtaagg tctgcaaccc aaacaaccca 360
caagaatgtt tgttgttgga cccaggtttg aacgaaatta tggagaagtc cttggactac 420
aacgagcgtt tgtgggcctg ggaaggttgg agatccgaag ttggtaagca attgagacca 480
ttgtacgaag aatacgtcgt tttgaagaac gagatggccg gtgctaacca ttacaaggat 540
tacggtgatt actggagagg tgattacgaa gttaacggtg ttgatggtta cgataacaac 600
agagatcaat tgatcgagga cgtcgagaga actttcgaag agatcaagcc attgtacgag 660
catttgcatg cttacgttag agctaagttg atgaacgctt acccatctta catttcccca 720
actggttgtt tgccagctca tttgttgggt gatatgtggg gtagattttg gactaacttg 780
tactctttga ccgtcccatt tggtcaaaag ccaaacattg atgtcactga cgctatggtt 840
aaccaagctt ggaacgctca aagaattttt aaggaggccg aaaagttttt cgtctccgtc 900
ggtttgccaa acatgactca aggtttttgg gaaaactcta tgttgaccga tccaggtaac 960
gttcaaaagg ttgtttgtca tccaactgcc tgggatttgg gtaagggtga ttttagaatt 1020
atcatgtgca ccaaggtcac catggatgac tttttgactg ctcatcatga aatgggtcat 1080
atccagtacg atatggccta cgctgctcaa ccatttttgt tgagaaacgg tgctaacgaa 1140
ggttttcatg aagctgttgg tgaaattatg tccttgtccg ctgctactcc aaagcatttg 1200
aagtctattg gtttgttgtc ccccgacttc caggaggata acgaaactga gattaacttc 1260
ttgttgaagc aggccttgac tatcgttggt actttgccat ttacttacat gttggagaag 1320
tggagatgga tggtttttaa gggtgaaatt ccaaaggacc agtggatgaa gaagtggtgg 1380
gaaatgaaga gagaaattgt cggtgttgtc gagccagtcc cacatgatga aacttactgt 1440
gatccagctt ctttgtttca tgtctctaac gattactcct tcatccgcta ctacactcgt 1500
actttgtacc agttccagtt tcaggaggct ttgtgccaag ctgctaagca tgaaggtcca 1560
ttgcataagt gtgatatttc caactctacc gaggccggtc aaaagttgtt gaacatgttg 1620
aagttgggtg agtccgaacc atggactttg gctttggaaa acgttgttgg tgctaagaac 1680
atgaacgtta gaccattgtt gaactacttc gagccattgt tcacttggtt gaaggatcag 1740
aacaagaact cttttgtcgg ttggtctact gactggtctc catacgctga tcaatccatt 1800
aaggtcagaa tctccttgaa gtctgctttg ggtgataagg cttacgaatg gaacgataac 1860
gaaatgtact tgttccgttc ctccgttgct tacgctatga gaacctactt tttggaaatt 1920
aagcaccaga ccatcttgtt cggtgaggaa gacgttagag ttgctgactt gaagccaaga 1980
atttctttta acttctacgt cactgccccc aagaacgtct ctgatattat tccacgtact 2040
gaggttgaag aagccatcag aatttcccgt tcccgtatta acgatgcttt cagattgaac 2100
gataactcct tggagttttt gggtatccaa accactttgg ctccaccata ccaatctcca 2160
gttact 2166
<210> 16
<211> 1791
<212> DNA
<213> Artificial Sequence
<220>
<223> MmACE2-615（恒河猴）核苷酸序列
<400> 16
tctaccattg aagaacaggc taagactttc ttggataagt ttaaccacga agccgaggat 60
ttgttttacc agtcctcttt ggcttcctgg aactacaaca ctaacattac tgaagagaac 120
gtccagaaca tgaacaacgc tggtgaaaag tggtctgctt ttttgaagga acaatccacc 180
ttggcccaaa tgtacccatt gcaagaaatt caaaacttga ctgtcaagtt gcagttgcag 240
gctttgcaac aaaacggttc ttctgttttg tctgaggata agtctaagcg tttgaacacc 300
attttgaaca ctatgtctac catctactcc accggtaagg tctgcaaccc aaacaaccca 360
caagaatgtt tgttgttgga cccaggtttg aacgaaatta tggagaagtc cttggactac 420
aacgagcgtt tgtgggcctg ggaaggttgg agatccgaag ttggtaagca attgagacca 480
ttgtacgaag aatacgtcgt tttgaagaac gagatggccg gtgctaacca ttacaaggat 540
tacggtgatt actggagagg tgattacgaa gttaacggtg ttgatggtta cgataacaac 600
agagatcaat tgatcgagga cgtcgagaga actttcgaag agatcaagcc attgtacgag 660
catttgcatg cttacgttag agctaagttg atgaacgctt acccatctta catttcccca 720
actggttgtt tgccagctca tttgttgggt gatatgtggg gtagattttg gactaacttg 780
tactctttga ccgtcccatt tggtcaaaag ccaaacattg atgtcactga cgctatggtt 840
aaccaagctt ggaacgctca aagaattttt aaggaggccg aaaagttttt cgtctccgtc 900
ggtttgccaa acatgactca aggtttttgg gaaaactcta tgttgaccga tccaggtaac 960
gttcaaaagg ttgtttgtca tccaactgcc tgggatttgg gtaagggtga ttttagaatt 1020
atcatgtgca ccaaggtcac catggatgac tttttgactg ctcatcatga aatgggtcat 1080
atccagtacg atatggccta cgctgctcaa ccatttttgt tgagaaacgg tgctaacgaa 1140
ggttttcatg aagctgttgg tgaaattatg tccttgtccg ctgctactcc aaagcatttg 1200
aagtctattg gtttgttgtc ccccgacttc caggaggata acgaaactga gattaacttc 1260
ttgttgaagc aggccttgac tatcgttggt actttgccat ttacttacat gttggagaag 1320
tggagatgga tggtttttaa gggtgaaatt ccaaaggacc agtggatgaa gaagtggtgg 1380
gaaatgaaga gagaaattgt cggtgttgtc gagccagtcc cacatgatga aacttactgt 1440
gatccagctt ctttgtttca tgtctctaac gattactcct tcatccgcta ctacactcgt 1500
actttgtacc agttccagtt tcaggaggct ttgtgccaag ctgctaagca tgaaggtcca 1560
ttgcataagt gtgatatttc caactctacc gaggccggtc aaaagttgtt gaacatgttg 1620
aagttgggtg agtccgaacc atggactttg gctttggaaa acgttgttgg tgctaagaac 1680
atgaacgtta gaccattgtt gaactacttc gagccattgt tcacttggtt gaaggatcag 1740
aacaagaact cttttgtcgg ttggtctact gactggtctc catacgctga t 1791
<210> 17
<211> 2166
<212> DNA
<213> Artificial Sequence
<220>
<223> MjACE2-740（穿山甲）核苷酸序列
<400> 17
tccacttctg atgaagaagc taagaccttt ttggagaagt ttaactccga agccgaagaa 60
ttgtcctacc agtcttcttt ggcttcttgg aactacaaca ctaacattac cgatgagaac 120
gtccagaaga tgaacgtcgc tggtgctaag tggtctactt tttacgaaga acaatccaag 180
atcgccaaga actaccagtt gcagaacatt cagaacgata ctattaagcg tcagttgcag 240
gctttgcaat tgtctggttc ttctgctttg tctgctgata agaaccaaag attgaacacc 300
attttgaaca ccatgtccac tatctactct accggtaagg tctgtaaccc aggtaaccca 360
caagaatgtt ctttgttgga accaggtttg gataacatta tggagtcctc taaggattac 420
aacgagcgtt tgtgggcttg ggaaggttgg cgttctgaag ttggtaagca attgagacca 480
ttgtacgaag aatacgtcgt cttgaagaac gaaatggcca gagctaacca ttacgaagat 540
tacggtgatt actggagagg tgattacgaa gctgaaggtg ctaacggtta caactactct 600
agagatcatt tgatcgagga cgtcgaacac atttttaccc agatcaagcc attgtacgag 660
catttgcatg cttacgttag agctaagttg atggataact acccctctca tatttcccca 720
accggttgtt tgccagctca tttgttgggt gatatgtggg gtagattttg gactaacttg 780
tacccattga ctgtcccatt tcgtcagaag ccaaacattg atgtcactga tgctatggtt 840
aaccagactt gggatgctaa cagaattttt aaggaggccg agaagttctt tgtctccgtc 900
ggtttgccaa agatgaccca aactttttgg gaaaactcta tgttgaccga gccaggtgat 960
ggtagaaagg ttgtttgtca tccaactgct tgggatttgg gtaagcatga ttttagaatt 1020
aagatgtgca ccaaggtcac catggacgat ttcttgaccg cccatcatga aatgggtcat 1080
attcaatacg atatggccta cgctatgcaa ccatacttgt tgagaaacgg tgctaacgaa 1140
ggttttcatg aagctgttgg tgaaattatg tccttgtctg ctgctactcc aaagcatttg 1200
aagaacattg gtttgttgcc accagatttt tacgaggaca acgaaactga aatcaacttc 1260
ttgttgaagc aggccttgac cattgtcggt actttgccat ttacttacat gctggaaaag 1320
tggcgttgga tggttttttc cggtcaaatt ccaaaggagc agtggatgaa gaagtggtgg 1380
gaaatgaaga gagaaattgt cggtgttgtt gagccagttc cacatgatga aacttactgt 1440
gatccagctt ctttgtttca cgttgctaac gattactcct ttatccgtta ctacacccgt 1500
actatttacc agtttcagtt tcaggaggcc ttgtgccaaa ccgccaagca tgaaggtcca 1560
ttgcataagt gtgatatttc caactccgcc gaagccggtc aaaagttgtt gcaaatgttg 1620
tctttgggta agtccaagcc atggactttg gctttggaaa gagttgttgg tactaagaac 1680
atggacgtta gaccattgtt gaactacttt gagccattgt tgacttggtt gaaggaacaa 1740
aacaagaact cctttgtcgg ttggaacact gattggtctc catacgctgc tcagtccatc 1800
aaggtcagaa tttctttgaa gtccgctttg ggtgaaaagg cctacgaatg gaacgattct 1860
gaaatgtact tgttccgttc ctccgtcgcc tacgctatga gagaatactt ttctaaggtt 1920
aagaagcaga ccatcccatt tgaggatgag tgtgttcgtg tctccgattt gaagccaaga 1980
gtttctttta ttttcttcgt caccttgccc aagaacgtct ccgccgttat tccaagagct 2040
gaagttgaag aagctattcg tatttctcgt tccagaatca acgacgcctt cagattggac 2100
gataactctt tggagttttt gggtattcag cccaccttgc aaccaccata ccaaccacca 2160
gttact 2166
<210> 18
<211> 1791
<212> DNA
<213> Artificial Sequence
<220>
<223> MjACE2-615（穿山甲）核苷酸序列
<400> 18
tccacttctg atgaagaagc taagaccttt ttggagaagt ttaactccga agccgaagaa 60
ttgtcctacc agtcttcttt ggcttcttgg aactacaaca ctaacattac cgatgagaac 120
gtccagaaga tgaacgtcgc tggtgctaag tggtctactt tttacgaaga acaatccaag 180
atcgccaaga actaccagtt gcagaacatt cagaacgata ctattaagcg tcagttgcag 240
gctttgcaat tgtctggttc ttctgctttg tctgctgata agaaccaaag attgaacacc 300
attttgaaca ccatgtccac tatctactct accggtaagg tctgtaaccc aggtaaccca 360
caagaatgtt ctttgttgga accaggtttg gataacatta tggagtcctc taaggattac 420
aacgagcgtt tgtgggcttg ggaaggttgg cgttctgaag ttggtaagca attgagacca 480
ttgtacgaag aatacgtcgt cttgaagaac gaaatggcca gagctaacca ttacgaagat 540
tacggtgatt actggagagg tgattacgaa gctgaaggtg ctaacggtta caactactct 600
agagatcatt tgatcgagga cgtcgaacac atttttaccc agatcaagcc attgtacgag 660
catttgcatg cttacgttag agctaagttg atggataact acccctctca tatttcccca 720
accggttgtt tgccagctca tttgttgggt gatatgtggg gtagattttg gactaacttg 780
tacccattga ctgtcccatt tcgtcagaag ccaaacattg atgtcactga tgctatggtt 840
aaccagactt gggatgctaa cagaattttt aaggaggccg agaagttctt tgtctccgtc 900
ggtttgccaa agatgaccca aactttttgg gaaaactcta tgttgaccga gccaggtgat 960
ggtagaaagg ttgtttgtca tccaactgct tgggatttgg gtaagcatga ttttagaatt 1020
aagatgtgca ccaaggtcac catggacgat ttcttgaccg cccatcatga aatgggtcat 1080
attcaatacg atatggccta cgctatgcaa ccatacttgt tgagaaacgg tgctaacgaa 1140
ggttttcatg aagctgttgg tgaaattatg tccttgtctg ctgctactcc aaagcatttg 1200
aagaacattg gtttgttgcc accagatttt tacgaggaca acgaaactga aatcaacttc 1260
ttgttgaagc aggccttgac cattgtcggt actttgccat ttacttacat gctggaaaag 1320
tggcgttgga tggttttttc cggtcaaatt ccaaaggagc agtggatgaa gaagtggtgg 1380
gaaatgaaga gagaaattgt cggtgttgtt gagccagttc cacatgatga aacttactgt 1440
gatccagctt ctttgtttca cgttgctaac gattactcct ttatccgtta ctacacccgt 1500
actatttacc agtttcagtt tcaggaggcc ttgtgccaaa ccgccaagca tgaaggtcca 1560
ttgcataagt gtgatatttc caactccgcc gaagccggtc aaaagttgtt gcaaatgttg 1620
tctttgggta agtccaagcc atggactttg gctttggaaa gagttgttgg tactaagaac 1680
atggacgtta gaccattgtt gaactacttt gagccattgt tgacttggtt gaaggaacaa 1740
aacaagaact cctttgtcgg ttggaacact gattggtctc catacgctgc t 1791
<210> 19
<211> 2166
<212> DNA
<213> Artificial Sequence
<220>
<223> MfACE2-740（土拨鼠）核苷酸序列
<400> 19
tctactatcg aggaattggc caagactttt ttggataagt ttaaccagga ggccgaggac 60
ttggattacc agcgttcttt ggcttcttgg aactacaaca ctaacattac caaggagaac 120
acccagaaga tgaacgaggc tgaagctaag tggtctgctt tttacgaaaa gcaatctaag 180
ttggcgaagg cctacccatt gcaagaaatt caaaacttta ccttgaagcg tcagttgcag 240
gctttgcaac aatccggttc ttctgctttg tctgctaaca agagagaaca attgaacacc 300
attttgaaca ccatgtccac catctactct accggtaagg tttgtaaccc aaagaagcca 360
caagaatgtt tgttgttgga acccggtttg gatggtatta tggctaactc tactgattac 420
aacgagcgtt tgtgggtttg ggaaggttgg agatccaagg ttggtaagca attgagacca 480
ttgtacgaag aatacgtcgt tttgaagaac gagatggcta gagctaacaa ctacgaagat 540
tacggtgatt actggagagg tgattacgaa gctgaaggtg ctgatggtta cggttacaac 600
cataaccaat tgattgagga cgttgagaga acttttgccg aaattaagcc attgtacgag 660
catttgcatg cctacgttag agctaagttg atgaacactt acccatctta catttccccc 720
actggttgtt tgccagctca tttgttgggt gatatgtggg gtagattttg gactaacttg 780
tactctttga ccgtcccatt tccagaaaag ccaaacattg acgttactga cgccatgatc 840
aagcagaact ggaacgctgt tagaattttc aaggaggctg aaaagttttt cgtttccgtt 900
ggtttgccaa acatgaccca gggtttttgg gaaaactcta tgttgaccga accaactgat 960
ggtagaaagg ttgtttgtca tccaactgct tgggatttgc aaaagggtga ttttagaatt 1020
aagatgtgca ccaaggtcac catggataac ttcttgactg ctcatcatga aatgggtcat 1080
attcagtaca acatggccta cgctattcag ccatacttgt tgagaaacgg tgctaacgaa 1140
ggttttcatg aagctgttgg tgaaattatg tccttgtctg ctactacccc aaagcatttg 1200
aagtctattg gtttgttgcc ctccgatttt cgtgaggata acgaaactga aattaacttc 1260
ttgttgaagc aggccttgac catcgttggt gctttgccat ttacttacat gttggaaaag 1320
tggcgttgga tggtttttaa gggtgaaatt ccaaaggacc agtggatgaa gaagtggtgg 1380
gaaatgaaga gagaaattgt cggtgttatg gagccagttc cacatgatga aacttactgt 1440
gatccagctg ctttgtacca tgtttctaac gatttttcct ttatccgtta ctacaccaga 1500
accatttacc agttccagtt tcaggaagct ttgtgtcaag ccgctaagca tgaaggtcca 1560
ttgcataagt gtgatatttc caactctacc gaggccggtc aaaagttgtt gaacatgttg 1620
agattgggta agtccaagcc atggactttg gctttggaaa acgttgttgg tgctagaaac 1680
atggatgtta gaccattgtt gaactacttc gagcccttgt ttggttggtt gaaggatcag 1740
aacagaaact cttttgtcgg ttggaacacc aactggtctc catacactga tcagtctatc 1800
aaggtcagaa tctctttgaa gtccgctttg ggtgaggaag cttaccaatg gaacgataac 1860
gaaatgtact tgttccgttc ttccgttgcc tacgctatga gaatgtactt ttctaaggtt 1920
aagaaccaga ccatcccctt cggtgaggaa gatgtttggg tttctgattt gaagccaaga 1980
atttccttta acttcttcgt caccacccca cagaacgctt ctgatattat tccaagaact 2040
gacgtcgaaa aggctattcg tatgtccaga ggtagaatta acggtgtctt tagattggac 2100
gataactcct tggaatttct gggtatccag ccaaccttgg gtccaccata ccaaccacca 2160
gttact 2166
<210> 20
<211> 1791
<212> DNA
<213> Artificial Sequence
<220>
<223> MfACE2-615（土拨鼠）核苷酸序列
<400> 20
tctactatcg aggaattggc caagactttt ttggataagt ttaaccagga ggccgaggac 60
ttggattacc agcgttcttt ggcttcttgg aactacaaca ctaacattac caaggagaac 120
acccagaaga tgaacgaggc tgaagctaag tggtctgctt tttacgaaaa gcaatctaag 180
ttggcgaagg cctacccatt gcaagaaatt caaaacttta ccttgaagcg tcagttgcag 240
gctttgcaac aatccggttc ttctgctttg tctgctaaca agagagaaca attgaacacc 300
attttgaaca ccatgtccac catctactct accggtaagg tttgtaaccc aaagaagcca 360
caagaatgtt tgttgttgga acccggtttg gatggtatta tggctaactc tactgattac 420
aacgagcgtt tgtgggtttg ggaaggttgg agatccaagg ttggtaagca attgagacca 480
ttgtacgaag aatacgtcgt tttgaagaac gagatggcta gagctaacaa ctacgaagat 540
tacggtgatt actggagagg tgattacgaa gctgaaggtg ctgatggtta cggttacaac 600
cataaccaat tgattgagga cgttgagaga acttttgccg aaattaagcc attgtacgag 660
catttgcatg cctacgttag agctaagttg atgaacactt acccatctta catttccccc 720
actggttgtt tgccagctca tttgttgggt gatatgtggg gtagattttg gactaacttg 780
tactctttga ccgtcccatt tccagaaaag ccaaacattg acgttactga cgccatgatc 840
aagcagaact ggaacgctgt tagaattttc aaggaggctg aaaagttttt cgtttccgtt 900
ggtttgccaa acatgaccca gggtttttgg gaaaactcta tgttgaccga accaactgat 960
ggtagaaagg ttgtttgtca tccaactgct tgggatttgc aaaagggtga ttttagaatt 1020
aagatgtgca ccaaggtcac catggataac ttcttgactg ctcatcatga aatgggtcat 1080
attcagtaca acatggccta cgctattcag ccatacttgt tgagaaacgg tgctaacgaa 1140
ggttttcatg aagctgttgg tgaaattatg tccttgtctg ctactacccc aaagcatttg 1200
aagtctattg gtttgttgcc ctccgatttt cgtgaggata acgaaactga aattaacttc 1260
ttgttgaagc aggccttgac catcgttggt gctttgccat ttacttacat gttggaaaag 1320
tggcgttgga tggtttttaa gggtgaaatt ccaaaggacc agtggatgaa gaagtggtgg 1380
gaaatgaaga gagaaattgt cggtgttatg gagccagttc cacatgatga aacttactgt 1440
gatccagctg ctttgtacca tgtttctaac gatttttcct ttatccgtta ctacaccaga 1500
accatttacc agttccagtt tcaggaagct ttgtgtcaag ccgctaagca tgaaggtcca 1560
ttgcataagt gtgatatttc caactctacc gaggccggtc aaaagttgtt gaacatgttg 1620
agattgggta agtccaagcc atggactttg gctttggaaa acgttgttgg tgctagaaac 1680
atggatgtta gaccattgtt gaactacttc gagcccttgt ttggttggtt gaaggatcag 1740
aacagaaact cttttgtcgg ttggaacacc aactggtctc catacactga t 1791
<210> 21
<211> 2166
<212> DNA
<213> Artificial Sequence
<220>
<223> PlACE2-740（果子狸）核苷酸序列
<400> 21
tctactactg aagagttggc caagactttt ttggaaactt tcaactacga ggcccaagag 60
ttgtcttacc aatcttctgt tgcttcttgg aactacaaca ctaacattac cgatgagaac 120
gccaagaaca tgaacgaagc tggtgctaag tggtctgctt actacgaaga acaatctaag 180
ttggcccaaa cttacccatt ggctgaaatt caagatgcca agattaagcg tcagttgcag 240
gctttgcaac agtctggttc ttctgttttg tctgctgata agtctcaacg tttgaacact 300
attttgaacg ccatgtctac tatctactcc actggtaagg cttgtaaccc aaacaaccca 360
caagaatgtt tgttgttgga accaggtttg gataacatta tggagaactc caaggactac 420
aacgaacgtt tgtgggcttg ggaaggttgg agagctgaag ttggtaagca attgagacca 480
ttgtacgaag aatacgtcgc tttgaagaac gagatggcca gagctaacaa ctacgaagat 540
tacggtgatt actggagagg tgattacgaa gaagaatgga ctggtggtta caactactct 600
agaaaccaat tgattcagga cgtcgaggac acttttgaac aaattaagcc attgtaccag 660
cacttgcacg cctacgttag agccaagttg atggatactt acccatctag aatttcccgt 720
accggttgtt tgccagctca tttgttgggt gatatgtggg gtagattttg gactaacttg 780
tacccattga ctgtcccatt tggtcaaaag ccaaacattg atgttaccga cgctatggtt 840
aaccagaact gggatgctag aagaattttc aaggaggccg aaaagttttt cgtctccgtt 900
ggtttgccaa acatgaccca aggtttttgg gaaaactcta tgttgactga gcccggcgat 960
ggtagaaagg ttgtttgtca tccaactgct tgggatttgg gtaagggtga ttttagaatt 1020
aagatgtgca ccaaggttac catggacgac tttttgactg ctcatcatga aatgggtcat 1080
atccagtacg atatggccta cgctgctcaa ccatttttgt tgagaaacgg tgctaacgaa 1140
ggttttcatg aagctgttgg tgaaattatg tccttgtctg ccgctactcc aaaccatttg 1200
aagactattg gtttgttgtc cccagccttt tccgaggaca acgaaactga gattaacttc 1260
ttgttgaagc aggccttgac cattgtcggt actttgccat ttacttacat gttggaaaag 1320
tggcgttgga tggtttttaa gggtgctatt ccaaaggaac agtggatgca aaagtggtgg 1380
gaaatgaaga gaaacattgt tggtgttgtc gagccagttc cacatgatga aacttactgt 1440
gatccagctt ctttgtttca cgttgccaac gattactctt ttatccgtta ctacacccgt 1500
accatttacc aatttcagtt ccaggaggct ttgtgccaaa ttgctaagca tgaaggtcca 1560
ttgcataagt gtgatatttc caactctact gaggccggta agaagttgtt ggaaatgttg 1620
tctttgggcc gttctgaacc atggactttg gctttggaaa gagttgttgg tgctaagaac 1680
atgaacgtta ctccattgtt gaactacttc gagccattgt ttacctggtt gaaggaacag 1740
aaccgtaact cttttgttgg ttgggacacc gattggagac catactctga tcagtccatc 1800
aaggttagaa tctctttgaa gtccgctttg ggtgagaagg cttacgaatg gaacgataac 1860
gaaatgtact tgttccgttc ctccattgcc tacgctatgc gtgaatactt ttctaaggtt 1920
aagaaccaga ccatcccctt cgttgaggat aacgtttggg tttctgattt gaagccaaga 1980
atttccttca acttcttcgt caccttttcc aacaacgttt ccgacgttat tccacgttct 2040
gaggttgaag atgctattcg catgtcccgt tctagaatta acgatgcctt tagattggac 2100
gacaactcct tggaattttt gggtatcgag ccaactttgt ctccaccata cagaccacca 2160
gttact 2166
<210> 22
<211> 1791
<212> DNA
<213> Artificial Sequence
<220>
<223> PlACE2-615（果子狸）核苷酸序列
<400> 22
tctactactg aagagttggc caagactttt ttggaaactt tcaactacga ggcccaagag 60
ttgtcttacc aatcttctgt tgcttcttgg aactacaaca ctaacattac cgatgagaac 120
gccaagaaca tgaacgaagc tggtgctaag tggtctgctt actacgaaga acaatctaag 180
ttggcccaaa cttacccatt ggctgaaatt caagatgcca agattaagcg tcagttgcag 240
gctttgcaac agtctggttc ttctgttttg tctgctgata agtctcaacg tttgaacact 300
attttgaacg ccatgtctac tatctactcc actggtaagg cttgtaaccc aaacaaccca 360
caagaatgtt tgttgttgga accaggtttg gataacatta tggagaactc caaggactac 420
aacgaacgtt tgtgggcttg ggaaggttgg agagctgaag ttggtaagca attgagacca 480
ttgtacgaag aatacgtcgc tttgaagaac gagatggcca gagctaacaa ctacgaagat 540
tacggtgatt actggagagg tgattacgaa gaagaatgga ctggtggtta caactactct 600
agaaaccaat tgattcagga cgtcgaggac acttttgaac aaattaagcc attgtaccag 660
cacttgcacg cctacgttag agccaagttg atggatactt acccatctag aatttcccgt 720
accggttgtt tgccagctca tttgttgggt gatatgtggg gtagattttg gactaacttg 780
tacccattga ctgtcccatt tggtcaaaag ccaaacattg atgttaccga cgctatggtt 840
aaccagaact gggatgctag aagaattttc aaggaggccg aaaagttttt cgtctccgtt 900
ggtttgccaa acatgaccca aggtttttgg gaaaactcta tgttgactga gcccggcgat 960
ggtagaaagg ttgtttgtca tccaactgct tgggatttgg gtaagggtga ttttagaatt 1020
aagatgtgca ccaaggttac catggacgac tttttgactg ctcatcatga aatgggtcat 1080
atccagtacg atatggccta cgctgctcaa ccatttttgt tgagaaacgg tgctaacgaa 1140
ggttttcatg aagctgttgg tgaaattatg tccttgtctg ccgctactcc aaaccatttg 1200
aagactattg gtttgttgtc cccagccttt tccgaggaca acgaaactga gattaacttc 1260
ttgttgaagc aggccttgac cattgtcggt actttgccat ttacttacat gttggaaaag 1320
tggcgttgga tggtttttaa gggtgctatt ccaaaggaac agtggatgca aaagtggtgg 1380
gaaatgaaga gaaacattgt tggtgttgtc gagccagttc cacatgatga aacttactgt 1440
gatccagctt ctttgtttca cgttgccaac gattactctt ttatccgtta ctacacccgt 1500
accatttacc aatttcagtt ccaggaggct ttgtgccaaa ttgctaagca tgaaggtcca 1560
ttgcataagt gtgatatttc caactctact gaggccggta agaagttgtt ggaaatgttg 1620
tctttgggcc gttctgaacc atggactttg gctttggaaa gagttgttgg tgctaagaac 1680
atgaacgtta ctccattgtt gaactacttc gagccattgt ttacctggtt gaaggaacag 1740
aaccgtaact cttttgttgg ttgggacacc gattggagac catactctga t 1791
<210> 23
<211> 2166
<212> DNA
<213> Artificial Sequence
<220>
<223> PsACE2-740（中华鳖）核苷酸序列
<400> 23
gatatcaccc aagaggccat taactttttg tccgaattta acgttcaggc cgaagatttg 60
tcttacgctt cttctttggc ttcttggaac tacaacacta acattaccga tgagaacgcc 120
aagaagatga acgaggctgg tgctaagtgg tctgtttttt acgatgaagc ttctaccaac 180
gcctccaagt acgctattga taagatcacc aaccacactg tcaagttgca attgcaatct 240
ttgcaaggta agggtacttc tgttttgtct ggtgaaaagt acaacgagtt gaacaagatt 300
ttgtccacca tgtctacctt ctactctact ggtactgttt gtaagccaga taacccagat 360
atttgcttgc cattggaacc aggtttggat gctattatgg cttcttctac tgattacttc 420
gagcgtttgt gggcctggga aggttggaga gctgatgttg gtaagaagat gagagaattg 480
tacgagagat acgtcgaatt ggagaacgag gccgctagat tgaacaagta ctctgattac 540
ggtgattact ggagaggtaa ctacgaagtt aacgatccaa ctgaatacgc ctactctaga 600
aaccaattga tggaggatgt tgaggccacc ttcgaacaga ttaagccatt gtacagagag 660
ttgcatgctt acgttagata cagattggaa aagttctacg gttccgacca tatctcctcc 720
actggttgtt tgccagccca tttgttgggt gatatgtggg gtagattttg gactaacttg 780
tacgctttga ctgtgccata cccagataag ccaaacattg atgttacttc tgagatggtc 840
aagaagaact ggaacgccac taagattttt aaggccgccg aagatttttt catgtccgtt 900
ggtttgtaca agatgaccga aggtttttgg aagaactcta tgattaccga gccaaacgat 960
ggtagaaagg ttgtttgtca tccaactgct tgggatatgg gtaagaagga ttacagaatt 1020
aagatgtgca ccaaggtctc tatggatgac tttttgaccg tccaccatga aatgggtcat 1080
attgaatacg atatggccta ctctaacttg tcctacttgt tgcgttctgg tgccaacgaa 1140
ggttttcatg aagctgttgg tgaaattatg tccttgtctg ctgctactcc aaagcatttg 1200
aagtctttgg atttgttgga gccaactttt caggaagata acgaaactga catcaacttc 1260
ttgttgaagc aggccttgac tattgttggt actatgccat ttacctacat gttggaaaag 1320
tggagatgga tggtttttaa gggtgatatt ccaaaggacg agtggatgaa gaagtggtgg 1380
gaaatgaaga gagctattgt tggtgttgtt gagccagttc cacatgatga aacttactgt 1440
gatccagctg ctttgtttca tgttgctaac gattactctt tcatccgtta ctacaccaga 1500
accatttacc agtttcagtt tcaggaggcc ttgtgcaagg ctgctaacca tggtggtttg 1560
ttgcatactt gtgatattac caactccatg gccgctggtc aaaagttgag agatatgttg 1620
gctttgggta gatcccaacc atggactaag gctttggaat ctattactgg tgaaaagaag 1680
atgaacgcca ccccattgtt gcattacttt gaaccattgt accagtggtt gattaagaac 1740
aactctggta gagctgttgg ttggaacact ttttggtctc catactctgg taacgctatc 1800
aaggtcagaa tctctttgaa gaccgctttg ggtgataacg cttacgaatg ggatgaaaac 1860
gaattgtact ttttcaagtc ctccatcgcc tacgctatga gaaagtactt tttggaggtc 1920
aagaaccaga ccgtctcctt tcaatgtact gatattcatg tctgggccgt tacccaacgt 1980
gtttcttttt actttgctgt ctctatgcca ggtaacgcta ctgattttat tccaaagtct 2040
gaggtcgaga ccgctatcag aatgtccaga ggtagaatta acgaagcctt tcgtttggac 2100
gataacacct tggaatttga gggtttgttg ccaactttgg cttctccata cgaaccacca 2160
gttact 2166
<210> 24
<211> 1791
<212> DNA
<213> Artificial Sequence
<220>
<223> PsACE2-615（中华鳖）核苷酸序列
<400> 24
gatatcaccc aagaggccat taactttttg tccgaattta acgttcaggc cgaagatttg 60
tcttacgctt cttctttggc ttcttggaac tacaacacta acattaccga tgagaacgcc 120
aagaagatga acgaggctgg tgctaagtgg tctgtttttt acgatgaagc ttctaccaac 180
gcctccaagt acgctattga taagatcacc aaccacactg tcaagttgca attgcaatct 240
ttgcaaggta agggtacttc tgttttgtct ggtgaaaagt acaacgagtt gaacaagatt 300
ttgtccacca tgtctacctt ctactctact ggtactgttt gtaagccaga taacccagat 360
atttgcttgc cattggaacc aggtttggat gctattatgg cttcttctac tgattacttc 420
gagcgtttgt gggcctggga aggttggaga gctgatgttg gtaagaagat gagagaattg 480
tacgagagat acgtcgaatt ggagaacgag gccgctagat tgaacaagta ctctgattac 540
ggtgattact ggagaggtaa ctacgaagtt aacgatccaa ctgaatacgc ctactctaga 600
aaccaattga tggaggatgt tgaggccacc ttcgaacaga ttaagccatt gtacagagag 660
ttgcatgctt acgttagata cagattggaa aagttctacg gttccgacca tatctcctcc 720
actggttgtt tgccagccca tttgttgggt gatatgtggg gtagattttg gactaacttg 780
tacgctttga ctgtgccata cccagataag ccaaacattg atgttacttc tgagatggtc 840
aagaagaact ggaacgccac taagattttt aaggccgccg aagatttttt catgtccgtt 900
ggtttgtaca agatgaccga aggtttttgg aagaactcta tgattaccga gccaaacgat 960
ggtagaaagg ttgtttgtca tccaactgct tgggatatgg gtaagaagga ttacagaatt 1020
aagatgtgca ccaaggtctc tatggatgac tttttgaccg tccaccatga aatgggtcat 1080
attgaatacg atatggccta ctctaacttg tcctacttgt tgcgttctgg tgccaacgaa 1140
ggttttcatg aagctgttgg tgaaattatg tccttgtctg ctgctactcc aaagcatttg 1200
aagtctttgg atttgttgga gccaactttt caggaagata acgaaactga catcaacttc 1260
ttgttgaagc aggccttgac tattgttggt actatgccat ttacctacat gttggaaaag 1320
tggagatgga tggtttttaa gggtgatatt ccaaaggacg agtggatgaa gaagtggtgg 1380
gaaatgaaga gagctattgt tggtgttgtt gagccagttc cacatgatga aacttactgt 1440
gatccagctg ctttgtttca tgttgctaac gattactctt tcatccgtta ctacaccaga 1500
accatttacc agtttcagtt tcaggaggcc ttgtgcaagg ctgctaacca tggtggtttg 1560
ttgcatactt gtgatattac caactccatg gccgctggtc aaaagttgag agatatgttg 1620
gctttgggta gatcccaacc atggactaag gctttggaat ctattactgg tgaaaagaag 1680
atgaacgcca ccccattgtt gcattacttt gaaccattgt accagtggtt gattaagaac 1740
aactctggta gagctgttgg ttggaacact ttttggtctc catactctgg t 1791
<210> 25
<211> 2166
<212> DNA
<213> Artificial Sequence
<220>
<223> RnACE2-740（褐家鼠）核苷酸序列
<400> 25
tctttgattg aggaaaaggc cgaatctttc ttgaacaagt tcaaccaaga agccgaagac 60
ttgtcttacc agtcttcttt ggcttcctgg aactacaaca ctaacattac tgaagagaac 120
gcccagaaga tgaacgaggc tgctgctaag tggtctgctt tttacgaaga acaatctaag 180
atcgcccaga acttttcttt gcaagagatt cagaacgcca ctatcaagag acaattgaag 240
gctttgcaac agtctggttc ttctgctttg tctccagata agaacaagca attgaacacc 300
atcttgaaca ccatgtccac catctactcc actggtaagg tttgtaactc tatgaaccca 360
caagaatgct tcttgttgga gccaggtttg gatgaaatta tggctacttc tactgactac 420
aaccgtcgtt tgtgggcttg ggaaggttgg agagctgaag ttggtaagca attgagacca 480
ttgtacgaag aatacgtcgt cttgaagaac gagatggcca gagccaacaa ctacgaagat 540
tacggtgatt actggagagg tgattacgaa gctgaaggtg ttgaaggtta caactacaac 600
agaaaccaat tgatcgagga cgtcgagaac acttttaagg agattaagcc attgtacgag 660
cagttgcatg cttacgttag aaccaagttg atggaagttt acccctctta catttcccca 720
actggttgtt tgccagctca tttgttgggt gatatgtggg gtagattttg gactaacttg 780
tacccattga ctaccccatt tttgcagaag ccaaacattg acgttactga cgctatggtt 840
aaccaatctt gggatgctga aagaattttc aaggaggccg agaagttctt cgtctctgtt 900
ggtttgccac aaatgactcc aggtttttgg actaactcta tgttgactga accaggtgat 960
gatagaaagg ttgtttgtca tccaactgcc tgggatttgg gtcatggtga ttttagaatt 1020
aagatgtgca ccaaggtcac catggacaac tttttgaccg cccatcatga aatgggtcat 1080
attcaatacg atatggccta cgctaagcaa ccatttttgt tgagaaacgg tgctaacgaa 1140
ggttttcatg aagctgttgg tgaaattatg tccttgtctg ctgctactcc aaagcatttg 1200
aagtctattg gtttgttgcc atccaacttt caggaggaca acgaaactga gattaacttt 1260
ttgttgaagc aggccttgac cattgtcggt actttgccat ttacttacat gttggagaag 1320
tggagatgga tggtttttca agataagatc ccacgtgagc agtggactaa gaagtggtgg 1380
gaaatgaaga gagaaattgt cggtgttgtc gagccattgc cacatgatga aacttactgt 1440
gatccagctt ctttgtttca cgtctctaac gattactctt tcatccgtta ctacacccgt 1500
actatttacc agttccaatt ccaggaggcc ttgtgccagg ctgctaagca tgatggtcca 1560
ttgcataagt gtgatatttc caactctacc gaggccggtc aaaagttgtt gaacatgttg 1620
tctttgggta actccggtcc atggactttg gctttggaaa acgttgttgg ttctagaaac 1680
atggacgtta agccattgtt gaactacttc cagccattgt ttgtttggtt gaaggaacaa 1740
aaccgcaact ccaccgttgg ttggtctact gattggtctc catacgctga tcagtccatt 1800
aaggtcagaa tttccttgaa gtctgccttg ggtaagaacg cctacgaatg gactgataac 1860
gaaatgtact tgttccgttc ctccgtcgct tacgctatga gagaatactt ttccagagaa 1920
aagaaccaga ccgttccatt cggtgaggct gatgtttggg tttctgattt gaagccaaga 1980
gtttctttta acttcttcgt cacctcccca aagaacgtct ctgatatcat cccaagatcc 2040
gaagttgaag aagccattag aatgtctaga ggcagaatta acgacatctt cggtttgaac 2100
gacaactcct tggaattttt gggtatctac ccaaccttga agccaccata cgaaccacca 2160
gttact 2166
<210> 26
<211> 1791
<212> DNA
<213> Artificial Sequence
<220>
<223> RnACE2-615（褐家鼠）核苷酸序列
<400> 26
tctttgattg aggaaaaggc cgaatctttc ttgaacaagt tcaaccaaga agccgaagac 60
ttgtcttacc agtcttcttt ggcttcctgg aactacaaca ctaacattac tgaagagaac 120
gcccagaaga tgaacgaggc tgctgctaag tggtctgctt tttacgaaga acaatctaag 180
atcgcccaga acttttcttt gcaagagatt cagaacgcca ctatcaagag acaattgaag 240
gctttgcaac agtctggttc ttctgctttg tctccagata agaacaagca attgaacacc 300
atcttgaaca ccatgtccac catctactcc actggtaagg tttgtaactc tatgaaccca 360
caagaatgct tcttgttgga gccaggtttg gatgaaatta tggctacttc tactgactac 420
aaccgtcgtt tgtgggcttg ggaaggttgg agagctgaag ttggtaagca attgagacca 480
ttgtacgaag aatacgtcgt cttgaagaac gagatggcca gagccaacaa ctacgaagat 540
tacggtgatt actggagagg tgattacgaa gctgaaggtg ttgaaggtta caactacaac 600
agaaaccaat tgatcgagga cgtcgagaac acttttaagg agattaagcc attgtacgag 660
cagttgcatg cttacgttag aaccaagttg atggaagttt acccctctta catttcccca 720
actggttgtt tgccagctca tttgttgggt gatatgtggg gtagattttg gactaacttg 780
tacccattga ctaccccatt tttgcagaag ccaaacattg acgttactga cgctatggtt 840
aaccaatctt gggatgctga aagaattttc aaggaggccg agaagttctt cgtctctgtt 900
ggtttgccac aaatgactcc aggtttttgg actaactcta tgttgactga accaggtgat 960
gatagaaagg ttgtttgtca tccaactgcc tgggatttgg gtcatggtga ttttagaatt 1020
aagatgtgca ccaaggtcac catggacaac tttttgaccg cccatcatga aatgggtcat 1080
attcaatacg atatggccta cgctaagcaa ccatttttgt tgagaaacgg tgctaacgaa 1140
ggttttcatg aagctgttgg tgaaattatg tccttgtctg ctgctactcc aaagcatttg 1200
aagtctattg gtttgttgcc atccaacttt caggaggaca acgaaactga gattaacttt 1260
ttgttgaagc aggccttgac cattgtcggt actttgccat ttacttacat gttggagaag 1320
tggagatgga tggtttttca agataagatc ccacgtgagc agtggactaa gaagtggtgg 1380
gaaatgaaga gagaaattgt cggtgttgtc gagccattgc cacatgatga aacttactgt 1440
gatccagctt ctttgtttca cgtctctaac gattactctt tcatccgtta ctacacccgt 1500
actatttacc agttccaatt ccaggaggcc ttgtgccagg ctgctaagca tgatggtcca 1560
ttgcataagt gtgatatttc caactctacc gaggccggtc aaaagttgtt gaacatgttg 1620
tctttgggta actccggtcc atggactttg gctttggaaa acgttgttgg ttctagaaac 1680
atggacgtta agccattgtt gaactacttc cagccattgt ttgtttggtt gaaggaacaa 1740
aaccgcaact ccaccgttgg ttggtctact gattggtctc catacgctga t 1791
<210> 27
<211> 2166
<212> DNA
<213> Artificial Sequence
<220>
<223> RfACE2-740（马铁菊头蝠）核苷酸序列
<400> 27
tctaccactg aagatttggc caagaagttt ttggacgact tcaactccga ggctgaaaac 60
ttgtctcatc aatcttcttt ggcctcctgg gaatacaaca ctaacatttc tgacgagaac 120
gtccaaaaga tggatgaagc cggtgctaag tggtctgatt tttacgaaaa gcaatccaag 180
ttggccaaga acttttcttt ggaggaaatc cacaacgaca ccgttaagtt gcagttgcaa 240
attttgcagc aatccggttc tccagttttg tctgaagata agtctaagcg tttgaactcc 300
attttgaacg ccatgtctac catctactcc actggtaagg tttgtaagcc aaacaaccca 360
caagaatgtt tgttgttgga accaggtttg gataacatta tgggcacttc taaggactac 420
aacgagcgtt tgtgggcttg ggaaggttgg agagctgaag ttggtaagca attgagacca 480
ttgtacgaag aatacgtcgt tttgaagaac gagatggcca gaggttacca ttacgaagat 540
tacggtgatt actggagaag agattacgaa actgaaggtt ctccagattt ggaatactct 600
agagatcaat tgatcaagga cgtcgagcgt attttcgccg agatcaagcc attgtacgaa 660
caattgcatg cctacgttag aaccaagttg atggatactt acccctttca catttcccca 720
actggttgtt tgccagctca tttgttgggt gatatgtggg gtagattttg gactaacttg 780
tacccattga ctgtcccatt tggtcaaaag ccaaacattg atgttaccga cgccatgttg 840
aaccagaact gggatgctaa gagaattttt aaggaggccg agaagttctt cgtctctatt 900
ggtttgccaa acatgaccga aggtttttgg aacaactcta tgttgactga cccaggtgat 960
ggtagaaagg ttgtttgtca tccaactgct tgggatttgg gtaagggtga ttttagaatt 1020
aagatgtgca ccaaggtcac catggaggat tttttgactg cccatcatga aatgggtcat 1080
attcaatacg atatggccta cgcttctcaa ccatacttgt tgagaaacgg tgctaacgaa 1140
ggttttcatg aagctgttgg tgaagttatg tctttgtctg ttgctactcc aaagcatttg 1200
aagactatgg gtttgttgtc ttccgacttt ttggaagata acgagactga aatcaacttc 1260
ttgttcaagc aggccttgaa cattgttggt accttgccat ttacttacat gttggaaaag 1320
tggcgctgga tggtttttaa gggtgaaatt ccaaaggagg agtggatgaa gaagtggtgg 1380
gaaatgaaga gaaagattgt cggtgttgtt gagccagttc cacatgatga aacttactgt 1440
gatccagctt ctttgtttca cgttgccaac gattactctt ttatccgtta ctacactcgt 1500
accatcttcg agttccagtt tcatgaggct ttgtgtcgta ttgctaagca tgatggtcca 1560
ttgcataagt gtgatatttc caactccacc gatgccggtg agaagttgca tcaaatgttg 1620
tctgttggta agtcccaacc atggacttct gttttgaagg attttgtcgg ttctaagaac 1680
atggacgttg gtccattgtt gagatacttt gaaccattgt acacctggtt gaccgaacaa 1740
aacagaaagt cctttgtcgg ttggaacact gattggtctc catacgctga tcagtccatt 1800
aaggttcgta tttccttgaa gtccgctttg ggtgaaaagg cttacgaatg gaacaacaac 1860
gaaatgtact tgttccgttc ctctgtcgct tacgccatga gagaatactt tttgaagacc 1920
aagaaccaga ccattttgtt cggtgaggaa gatgtttggg tctctaactt gaagccaaga 1980
atttccttta acttctacgt cacttcccca cgtaacttgt ctgacattat tccaaagcca 2040
gaagtcgaag gtgctattag aatgtctaga tccagaatca acgacgcctt ccgtttggat 2100
gataactctt tggagttttt gggcatccag ccaaccttgg gtccaccata ccaaccacca 2160
gttact 2166
<210> 28
<211> 1791
<212> DNA
<213> Artificial Sequence
<220>
<223> RfACE2-615（马铁菊头蝠）核苷酸序列
<400> 28
tctaccactg aagatttggc caagaagttt ttggacgact tcaactccga ggctgaaaac 60
ttgtctcatc aatcttcttt ggcctcctgg gaatacaaca ctaacatttc tgacgagaac 120
gtccaaaaga tggatgaagc cggtgctaag tggtctgatt tttacgaaaa gcaatccaag 180
ttggccaaga acttttcttt ggaggaaatc cacaacgaca ccgttaagtt gcagttgcaa 240
attttgcagc aatccggttc tccagttttg tctgaagata agtctaagcg tttgaactcc 300
attttgaacg ccatgtctac catctactcc actggtaagg tttgtaagcc aaacaaccca 360
caagaatgtt tgttgttgga accaggtttg gataacatta tgggcacttc taaggactac 420
aacgagcgtt tgtgggcttg ggaaggttgg agagctgaag ttggtaagca attgagacca 480
ttgtacgaag aatacgtcgt tttgaagaac gagatggcca gaggttacca ttacgaagat 540
tacggtgatt actggagaag agattacgaa actgaaggtt ctccagattt ggaatactct 600
agagatcaat tgatcaagga cgtcgagcgt attttcgccg agatcaagcc attgtacgaa 660
caattgcatg cctacgttag aaccaagttg atggatactt acccctttca catttcccca 720
actggttgtt tgccagctca tttgttgggt gatatgtggg gtagattttg gactaacttg 780
tacccattga ctgtcccatt tggtcaaaag ccaaacattg atgttaccga cgccatgttg 840
aaccagaact gggatgctaa gagaattttt aaggaggccg agaagttctt cgtctctatt 900
ggtttgccaa acatgaccga aggtttttgg aacaactcta tgttgactga cccaggtgat 960
ggtagaaagg ttgtttgtca tccaactgct tgggatttgg gtaagggtga ttttagaatt 1020
aagatgtgca ccaaggtcac catggaggat tttttgactg cccatcatga aatgggtcat 1080
attcaatacg atatggccta cgcttctcaa ccatacttgt tgagaaacgg tgctaacgaa 1140
ggttttcatg aagctgttgg tgaagttatg tctttgtctg ttgctactcc aaagcatttg 1200
aagactatgg gtttgttgtc ttccgacttt ttggaagata acgagactga aatcaacttc 1260
ttgttcaagc aggccttgaa cattgttggt accttgccat ttacttacat gttggaaaag 1320
tggcgctgga tggtttttaa gggtgaaatt ccaaaggagg agtggatgaa gaagtggtgg 1380
gaaatgaaga gaaagattgt cggtgttgtt gagccagttc cacatgatga aacttactgt 1440
gatccagctt ctttgtttca cgttgccaac gattactctt ttatccgtta ctacactcgt 1500
accatcttcg agttccagtt tcatgaggct ttgtgtcgta ttgctaagca tgatggtcca 1560
ttgcataagt gtgatatttc caactccacc gatgccggtg agaagttgca tcaaatgttg 1620
tctgttggta agtcccaacc atggacttct gttttgaagg attttgtcgg ttctaagaac 1680
atggacgttg gtccattgtt gagatacttt gaaccattgt acacctggtt gaccgaacaa 1740
aacagaaagt cctttgtcgg ttggaacact gattggtctc catacgctga t 1791
<210> 29
<211> 2166
<212> DNA
<213> Artificial Sequence
<220>
<223> sACE2-740（蝾螈）核苷酸序列
<400> 29
gacgttacta acgatgctag agtctttttg gacgctttta acgctcaagc tgaagatttg 60
tcttacgaga actctttggc ttcctgggct tacaacacta acattactga agagaacgcc 120
atcaagatga acgaagccgg tgctaagtgg actgcttttt acaagaaggc taacaacaac 180
gcctctagat ttccagttga tcaaattacc gatcccgaca ttaagttgca gattttgtcc 240
ttgggtgaga agggttcctc cgtcttgcca gatgataagt acaacagatt gaacaaggcc 300
ttgtctgaca tgtccaccat ttactctact ggtactgttt gtgacaactc cgctaagtgt 360
ttgcagttgg aaccaggttt ggatttgatt atggctgatt ctactgacta ccacaagcgt 420
ttgtgggcct gggaaggttg gagatccgaa gttggtaaga agatgagacc attgtacgaa 480
acttacgtcg atttgaacaa cgaagccgcc aagttgaacg attacgctga ttacggtgat 540
tactggagag gtaactacga aactcaagat tctggtaagt acgcctactc tagaaacgat 600
ttgaagagag atgtcgagcg tacttttaag gagatccagc cattgtacag agaattgcat 660
gcctacgtta gagataagtt gcgtggtgtt tacggtgata agtacatttc taagaacggt 720
tgcttgccag ctcatttgtt gggtgatatg tggggtagat tttggactaa cttgtaccca 780
ttggctgttc catacccaaa ccaaccatct attgatgtta cttccgccat gaacgctaag 840
aagtggaacg ttgataagat gtttcgtgag gccgaggact tctttgtttc tgtcggtttg 900
tacaagatga acgagaactt ctggaacttc tctatgttga ctgagccaaa cgacggtaga 960
aacgttgttt gtcatccaac tgcttgggat atgggtaaga acgattttag aattaagatg 1020
tgcaccaagg tgaacatgga ggacttcttg accgtccacc atgagatggg tcacattcaa 1080
tacgatatgg cttacgctaa cttgtccttt ttgttgcgta acggtgctaa cgagggtttt 1140
catgaagctg ttggtgaaat tatgtccttg tctgctgcta ctccaaagca tttgaagtct 1200
ttggatttgt tgccaccaac ttttgtggag aacgaagaaa ccaacatcaa cttcttgttg 1260
cgccaggctt tgactattgt cgccaccatg ccatttactt acatgttgga agaatggaga 1320
tggaaggttt ttaacggtga aattccacgt gaccagtgga tgaagaagtg gtggcaaatg 1380
aagagagaaa ttgtcggtgt tatggagcca gttccacatg atgaaactta ctgtgatcca 1440
gctgctttgt ttcatgttgc taacgattac tctttcattc gctactacac ccgtactatc 1500
taccagtttc aatttcagga ggccttgtgc aaggccgcta accataacgg ttctttgcat 1560
acttgtgata tcaccaactc caccttggct ggtcaaaagt tgagaactat gttggctttg 1620
ggtaactcta agccatggac tatggctttg gaatctatta ctggtggtaa gactatggac 1680
gcccaaccat tgttgcatta ctttgaccca ttgtacactt ggttgagaaa gaacaacatt 1740
gacaacaacc gtcagaccta ctgggatact gaatggtctg cttacactga ttacgagatt 1800
aaggttcgta tctctttgca ctccgctttc ggtgacaacg cctacacttg ggattctggt 1860
gaacaatact tgtttaagtc caccatcgcc tacgctatga ttaagtacta ctctgaagtc 1920
aagagcgagc aggtcccatt tactgctgaa aacgtttttg ttacccgtga gaccttgaga 1980
atttcctttt acttccacgt cactgaccca cgtaacattt cctcttttat cccaaagatc 2040
gacgtcgaag atgccgttag attgtctaga ggtagaatta actctgcctt caacttggac 2100
gacaacactt tggaatttgt ggacatcttg tccaccttgt ccccatccgt tgaaccacca 2160
gttact 2166
<210> 30
<211> 1791
<212> DNA
<213> Artificial Sequence
<220>
<223> sACE2-615（蝾螈）核苷酸序列
<400> 30
gacgttacta acgatgctag agtctttttg gacgctttta acgctcaagc tgaagatttg 60
tcttacgaga actctttggc ttcctgggct tacaacacta acattactga agagaacgcc 120
atcaagatga acgaagccgg tgctaagtgg actgcttttt acaagaaggc taacaacaac 180
gcctctagat ttccagttga tcaaattacc gatcccgaca ttaagttgca gattttgtcc 240
ttgggtgaga agggttcctc cgtcttgcca gatgataagt acaacagatt gaacaaggcc 300
ttgtctgaca tgtccaccat ttactctact ggtactgttt gtgacaactc cgctaagtgt 360
ttgcagttgg aaccaggttt ggatttgatt atggctgatt ctactgacta ccacaagcgt 420
ttgtgggcct gggaaggttg gagatccgaa gttggtaaga agatgagacc attgtacgaa 480
acttacgtcg atttgaacaa cgaagccgcc aagttgaacg attacgctga ttacggtgat 540
tactggagag gtaactacga aactcaagat tctggtaagt acgcctactc tagaaacgat 600
ttgaagagag atgtcgagcg tacttttaag gagatccagc cattgtacag agaattgcat 660
gcctacgtta gagataagtt gcgtggtgtt tacggtgata agtacatttc taagaacggt 720
tgcttgccag ctcatttgtt gggtgatatg tggggtagat tttggactaa cttgtaccca 780
ttggctgttc catacccaaa ccaaccatct attgatgtta cttccgccat gaacgctaag 840
aagtggaacg ttgataagat gtttcgtgag gccgaggact tctttgtttc tgtcggtttg 900
tacaagatga acgagaactt ctggaacttc tctatgttga ctgagccaaa cgacggtaga 960
aacgttgttt gtcatccaac tgcttgggat atgggtaaga acgattttag aattaagatg 1020
tgcaccaagg tgaacatgga ggacttcttg accgtccacc atgagatggg tcacattcaa 1080
tacgatatgg cttacgctaa cttgtccttt ttgttgcgta acggtgctaa cgagggtttt 1140
catgaagctg ttggtgaaat tatgtccttg tctgctgcta ctccaaagca tttgaagtct 1200
ttggatttgt tgccaccaac ttttgtggag aacgaagaaa ccaacatcaa cttcttgttg 1260
cgccaggctt tgactattgt cgccaccatg ccatttactt acatgttgga agaatggaga 1320
tggaaggttt ttaacggtga aattccacgt gaccagtgga tgaagaagtg gtggcaaatg 1380
aagagagaaa ttgtcggtgt tatggagcca gttccacatg atgaaactta ctgtgatcca 1440
gctgctttgt ttcatgttgc taacgattac tctttcattc gctactacac ccgtactatc 1500
taccagtttc aatttcagga ggccttgtgc aaggccgcta accataacgg ttctttgcat 1560
acttgtgata tcaccaactc caccttggct ggtcaaaagt tgagaactat gttggctttg 1620
ggtaactcta agccatggac tatggctttg gaatctatta ctggtggtaa gactatggac 1680
gcccaaccat tgttgcatta ctttgaccca ttgtacactt ggttgagaaa gaacaacatt 1740
gacaacaacc gtcagaccta ctgggatact gaatggtctg cttacactga t 1791
<210> 31
<211> 2166
<212> DNA
<213> Artificial Sequence
<220>
<223> SsACE2-740（野猪）核苷酸序列
<400> 31
tctactaccg aggaattggc taagactttt ttggaaaagt tcaacttgga ggccgaggat 60
ttggcttacc aatcttcttt ggcttcttgg aactacaaca ctaacattac cgatgagaac 120
atccagaaga tgaacgacgc tagagccaag tggtctgctt tttacgaaga acaatctcgt 180
attgccaaga cctacccatt ggatgaaatt caaactttga tcttgaagcg tcagttgcag 240
gctttgcaac agtccggtac ttctggtttg tctgctgata agtctaagag attgaacacc 300
atcttgaaca ccatgtccac tatttactct tccggtaagg ttcttgatcc aaacaaccca 360
caagaatgtt tggttttgga accaggtttg gatgaaatta tggagaactc taaggactac 420
tcccgtagat tgtgggcttg ggaatcttgg agagctgaag ttggtaagca attgagacca 480
ttgtacgaag aatacgtcgt cttggagaac gagatggcta gagctaacaa ctacgaagat 540
tacggtgatt actggagagg tgattacgaa gttactggta ctggtgatta cgattactct 600
agaaaccaat tgatggagga cgttgagaga actttcgctg aaattaagcc attgtacgaa 660
cacttgcacg cctacgttag agctaagttg atggatgctt acccatctag aatttcccca 720
actggttgtt tgccagctca tttgttgggt gatatgtggg gtagattttg gactaacttg 780
tacccattga ctgtcccatt tggtgaaaag ccatctattg atgttaccga ggccatggtt 840
aaccagtctt gggatgctat tagaatcttt gaggaagcgg agaagttttt cgtctctatt 900
ggtttgccaa acatgaccca aggtttttgg aacaactcta tgttgactga gccaggtgat 960
ggtagaaagg ttgtttgtca tccaactgct tgggatttgg gtaagggtga ttttagaatt 1020
aagatgtgca ccaaggtcac catggatgat tttttgactg ctcatcatga gatgggtcac 1080
attcaatacg atatggccta cgctattcag ccatacttgt tgagaaacgg tgctaacgaa 1140
ggttttcatg aagctgttgg tgaaattatg tccttgtccg ctgctactcc acattacttg 1200
aaggctttgg gtttgttgcc accagatttt tacgaagatt ctgagactga aatcaacttc 1260
ttgttgaagc aggccttgac tattgtcggt actttgccat ttacttacat gttggaaaag 1320
tggcgttgga tggtttttaa gggtgaaatt ccaaaggagc agtggatgca aaagtggtgg 1380
gaaatgaaga gagaaattgt cggtgttgtc gagccattgc cacatgatga aacttactgt 1440
gatccagctt gtttgtttca cgtcgctgaa gattactctt tcatccgtta ctacacccgt 1500
actatttacc agtttcagtt ccatgaggct ttgtgtagaa ctgccaagca tgaaggtcca 1560
ttgtacaagt gtgatatttc caactctacc gaggctggtc aaaagttgtt gcaaatgttg 1620
tctttgggta agtccgaacc atggactttg gctttggaaa acattgttgg tgttaagacc 1680
atggacgtca agccattgtt gtcttacttt gagccattgt tgacctggtt gaaggcccaa 1740
aacggtaact cttctgttgg ttggaacact gattggactc catacgctga tcaatccatc 1800
aaggttagaa tctccttgaa gtccgctttg ggtaaggaag cctacgaatg gaacgataac 1860
gaaatgtact tgttccgctc ctccatcgcc tacgctatgc gtaactactt ttcttctgct 1920
aagaacgaga ccatcccatt tggtgctgaa gatgtttggg tttctgattt gaagccaaga 1980
atttccttta acttcttcgt cacctcccca gccaacatgt ccgatattat tccaagatcc 2040
gatgtcgaga aggccatttc tatgtctcgt tctagaatta acgacgcctt ccgtttggat 2100
gacaacactt tggaattttt gggtatccag ccaactttgg gtccaccaga tgaaccacca 2160
gttact 2166
<210> 32
<211> 1791
<212> DNA
<213> Artificial Sequence
<220>
<223> SsACE2-615（野猪）核苷酸序列
<400> 32
tctactaccg aggaattggc taagactttt ttggaaaagt tcaacttgga ggccgaggat 60
ttggcttacc aatcttcttt ggcttcttgg aactacaaca ctaacattac cgatgagaac 120
atccagaaga tgaacgacgc tagagccaag tggtctgctt tttacgaaga acaatctcgt 180
attgccaaga cctacccatt ggatgaaatt caaactttga tcttgaagcg tcagttgcag 240
gctttgcaac agtccggtac ttctggtttg tctgctgata agtctaagag attgaacacc 300
atcttgaaca ccatgtccac tatttactct tccggtaagg ttcttgatcc aaacaaccca 360
caagaatgtt tggttttgga accaggtttg gatgaaatta tggagaactc taaggactac 420
tcccgtagat tgtgggcttg ggaatcttgg agagctgaag ttggtaagca attgagacca 480
ttgtacgaag aatacgtcgt cttggagaac gagatggcta gagctaacaa ctacgaagat 540
tacggtgatt actggagagg tgattacgaa gttactggta ctggtgatta cgattactct 600
agaaaccaat tgatggagga cgttgagaga actttcgctg aaattaagcc attgtacgaa 660
cacttgcacg cctacgttag agctaagttg atggatgctt acccatctag aatttcccca 720
actggttgtt tgccagctca tttgttgggt gatatgtggg gtagattttg gactaacttg 780
tacccattga ctgtcccatt tggtgaaaag ccatctattg atgttaccga ggccatggtt 840
aaccagtctt gggatgctat tagaatcttt gaggaagcgg agaagttttt cgtctctatt 900
ggtttgccaa acatgaccca aggtttttgg aacaactcta tgttgactga gccaggtgat 960
ggtagaaagg ttgtttgtca tccaactgct tgggatttgg gtaagggtga ttttagaatt 1020
aagatgtgca ccaaggtcac catggatgat tttttgactg ctcatcatga gatgggtcac 1080
attcaatacg atatggccta cgctattcag ccatacttgt tgagaaacgg tgctaacgaa 1140
ggttttcatg aagctgttgg tgaaattatg tccttgtccg ctgctactcc acattacttg 1200
aaggctttgg gtttgttgcc accagatttt tacgaagatt ctgagactga aatcaacttc 1260
ttgttgaagc aggccttgac tattgtcggt actttgccat ttacttacat gttggaaaag 1320
tggcgttgga tggtttttaa gggtgaaatt ccaaaggagc agtggatgca aaagtggtgg 1380
gaaatgaaga gagaaattgt cggtgttgtc gagccattgc cacatgatga aacttactgt 1440
gatccagctt gtttgtttca cgtcgctgaa gattactctt tcatccgtta ctacacccgt 1500
actatttacc agtttcagtt ccatgaggct ttgtgtagaa ctgccaagca tgaaggtcca 1560
ttgtacaagt gtgatatttc caactctacc gaggctggtc aaaagttgtt gcaaatgttg 1620
tctttgggta agtccgaacc atggactttg gctttggaaa acattgttgg tgttaagacc 1680
atggacgtca agccattgtt gtcttacttt gagccattgt tgacctggtt gaaggcccaa 1740
aacggtaact cttctgttgg ttggaacact gattggactc catacgctga t 1791
<210> 33
<211> 2157
<212> DNA
<213> Artificial Sequence
<220>
<223> TeACE2-740（蛇）核苷酸序列
<400> 33
gatgttactc aacaagccgc tgaatttttg aagcaatttg acgccagagc cgacgatttg 60
tactacgctg cttctattgc ttcttggaac tacaacacta acttgaccga agaaaacgcc 120
aagattatgc acgaaaagga caacattttc tccaagtttt acgaggaggc ctctaagaac 180
gcctctatgt acaacgttaa ccaaattacc aacgagacca ttcgtttgca gttgcacttg 240
ttgcagaacg tcccaactaa ctcctctact aaggatcaat tggataccgt tttgcgtaag 300
atgtctacta tgtactccac tggtaccgtt tgtaagcaag atgatccatt taactgcttg 360
cccttggagc caggtttgga tgatattatg gaaaacaact ggtcctactc cgaacgtttg 420
tgggcttggg aaggttggag agctgatgtt ggtaagaaga tgagaccatt gtacgaatct 480
tacgtcgagt tgaagaacaa gtacgctaga ttgagaggtt acgctgatta cggtgattac 540
tggagagcta actacgaagt tgatttgcca aaggaatacc agtaccagag agcccaattg 600
atcactgacg tcgaaaacac tttgcaacag attatgccat tgtacaagca cttgcacgct 660
tacgttagaa gacatttgta caagcattac ggtcccgaat ttatcaactt ggagggtgcc 720
attcccgccc atttgttggg tgatatgtgg ggtagatttt ggactaactt gtacccattg 780
atggtcccat ttccaaacaa gacttctatt gacgtcacct ccgctatggt caccaagaag 840
tggactgtta actctatttt caaggccgct gagcaatttt tcacctccat tggtttgttt 900
ccaatgaccg ataacttttg gaacaactcc atgttggaag agccaaagga tggtagaaag 960
gttgtttgtc atccaactgc ttgggatatg ggtaagaagg attacagaat taagatgtgc 1020
accaagatca acatggagga cttcttgacc gctcaccatg aaatgggtca tattgaatac 1080
gacatggcct actctgatca gccatttttg ttgagaaacg gtgctaacga aggttttcat 1140
gaagctgttg gtgaaattat gtccttgtct gccgctactc caaagtactt gaagtctttg 1200
ggtttgttgg aacacacctt tcaagaggat actgaaaccg atatcaactt tttgttgaag 1260
caggccttga ccattgtcgg tactatgcca tttacttaca tgttggaaaa gtggcgttgg 1320
atggtttttg ctgaacaaat tccaaaggat cagtggatga agaagtggtg ggaaatgaag 1380
agagaaattg tcggtgttgt tgagccattg ccacataacg aagaatactg tgatccagct 1440
gctttgtttc atgttgctaa cgattactct ttcatccgtt actacaccag aaccatctac 1500
cagtttcagt tccaggaggc tttgtgtcaa gctgccggtc atactggtga attgtacaag 1560
tgtgaaattt cccactccac cgacgccggt catattttga aggatatgtt ggctttgggt 1620
tcctctcaac catggactaa ggctttggaa tctattacta agtcccagaa gatggacgcc 1680
accccattta gacattactt tgacccattg ttgaagtggt tggaaaagca aaactctaac 1740
gagaacgtcg gctggaacgt taactggact ccatactcta agtacgccat caaggttaga 1800
atctctttga agagagcttt gggcgatgat gcttacaact ggactgcttc tgaaatgtac 1860
ttgtttaagt ccaccatcgc ctacgccatg caaaagtact tcttggagat taagaacaag 1920
accgtcttgt tccagaccga caacgttcat gtctctccag ttactgagag aatttctttt 1980
tacttcaccg tctccatgcc aaccaacatc tctgaattgg ttccaaagtc tgaagtcgag 2040
gaagccattt ctttgtctag agatagaatt aacgaggcct ttcgtttgac cgaccagact 2100
ttggagtttg ttggtttgtt gccaactttg gctccaccat acgaatctcc aattact 2157
<210> 34
<211> 1785
<212> DNA
<213> Artificial Sequence
<220>
<223> TeACE2-615（蛇）核苷酸序列
<400> 34
gatgttactc aacaagccgc tgaatttttg aagcaatttg acgccagagc cgacgatttg 60
tactacgctg cttctattgc ttcttggaac tacaacacta acttgaccga agaaaacgcc 120
aagattatgc acgaaaagga caacattttc tccaagtttt acgaggaggc ctctaagaac 180
gcctctatgt acaacgttaa ccaaattacc aacgagacca ttcgtttgca gttgcacttg 240
ttgcagaacg tcccaactaa ctcctctact aaggatcaat tggataccgt tttgcgtaag 300
atgtctacta tgtactccac tggtaccgtt tgtaagcaag atgatccatt taactgcttg 360
cccttggagc caggtttgga tgatattatg gaaaacaact ggtcctactc cgaacgtttg 420
tgggcttggg aaggttggag agctgatgtt ggtaagaaga tgagaccatt gtacgaatct 480
tacgtcgagt tgaagaacaa gtacgctaga ttgagaggtt acgctgatta cggtgattac 540
tggagagcta actacgaagt tgatttgcca aaggaatacc agtaccagag agcccaattg 600
atcactgacg tcgaaaacac tttgcaacag attatgccat tgtacaagca cttgcacgct 660
tacgttagaa gacatttgta caagcattac ggtcccgaat ttatcaactt ggagggtgcc 720
attcccgccc atttgttggg tgatatgtgg ggtagatttt ggactaactt gtacccattg 780
atggtcccat ttccaaacaa gacttctatt gacgtcacct ccgctatggt caccaagaag 840
tggactgtta actctatttt caaggccgct gagcaatttt tcacctccat tggtttgttt 900
ccaatgaccg ataacttttg gaacaactcc atgttggaag agccaaagga tggtagaaag 960
gttgtttgtc atccaactgc ttgggatatg ggtaagaagg attacagaat taagatgtgc 1020
accaagatca acatggagga cttcttgacc gctcaccatg aaatgggtca tattgaatac 1080
gacatggcct actctgatca gccatttttg ttgagaaacg gtgctaacga aggttttcat 1140
gaagctgttg gtgaaattat gtccttgtct gccgctactc caaagtactt gaagtctttg 1200
ggtttgttgg aacacacctt tcaagaggat actgaaaccg atatcaactt tttgttgaag 1260
caggccttga ccattgtcgg tactatgcca tttacttaca tgttggaaaa gtggcgttgg 1320
atggtttttg ctgaacaaat tccaaaggat cagtggatga agaagtggtg ggaaatgaag 1380
agagaaattg tcggtgttgt tgagccattg ccacataacg aagaatactg tgatccagct 1440
gctttgtttc atgttgctaa cgattactct ttcatccgtt actacaccag aaccatctac 1500
cagtttcagt tccaggaggc tttgtgtcaa gctgccggtc atactggtga attgtacaag 1560
tgtgaaattt cccactccac cgacgccggt catattttga aggatatgtt ggctttgggt 1620
tcctctcaac catggactaa ggctttggaa tctattacta agtcccagaa gatggacgcc 1680
accccattta gacattactt tgacccattg ttgaagtggt tggaaaagca aaactctaac 1740
gagaacgtcg gctggaacgt taactggact ccatactcta agcat 1785
<210> 35
<211> 2178
<212> DNA
<213> Artificial Sequence
<220>
<223> CsACE2-740（银鲑鱼）核苷酸序列
<400> 35
tccgatttgg aaagacgtgc tcaagaattt ttgaaccagt tcgatggtaa cgctacccat 60
ttgatgtacc aatactcttt ggcttcttgg gcttacaaca ctgatatttc tcaagagaac 120
ttggacaagt tgggtgtcca atctgctatt tggggtgaat actactctac tgtttctaag 180
gaatccgaga agttcccaat cgaccaaatc agagatccat tgattaagtt gcagttgatc 240
tccttgcaag acaagggttc tggtgctttg tctgctgata aggctgctca tttgaacaag 300
gttatgaacg aaatgtcctc catttactcc accggtactg tttgtaagcg tgaagatcca 360
tttgattgtc agactttgga accaggtttg gaatctgtta tggctaacat ggattctgac 420
tactacgaga gattgcacgt ctgggaaggt tggagagttg aagttggtaa gaagatgaga 480
ccattgtacg aagattacgt cgatttgaag aacgaggctg ctaagttgaa cgattacgaa 540
gattacggtg attactggag atccaactac gaaactactg acgattctcc ctacaactac 600
gctagaggtc aattgatgac tgatgttaga agaatctaca aggagatcct gcccttgtac 660
aaggagttgc acgcctacgt tagatccaag ttgcaggcta agcatccaga acatattcac 720
ccagaaggtg gtttgccagc tcatttgttg ggtgatatgt ggggtagatt ttggactggt 780
ttgtacccaa tttctacccc atttccagaa aagatcgata tcgacgttac taacgctatg 840
atcgcccaaa agtggccaaa ggatagattg tttcaagagg ctgaaaagtt cttcatgtcc 900
gtcggtttgt acaagatgtt tgacaacttt tggaaggact ccatgttgga aaagccaact 960
gatggtagaa aggttgtttg tcatccaact gcttgggata tgggtaacag agaagatttt 1020
agaatcaaga tgtgcaccga ggttaacatg gatcattttt tgaccgctca tcacgagatg 1080
ggtcataacc aataccaaat ggcttacaga aacttgtcct acttgttgcg tgacggtgct 1140
aacgaaggtt ttcatgaagc tgttggtgaa attatgtcct tgtccgctgc tactccaaag 1200
catttgaagg ctttgggttt gttgccagat gattttgttg aagacaagga gaccgaaatc 1260
aacttcttga tgaagcaggc cttgaccatt gttgctactt tgccatttac ttacatgttg 1320
gaggaatgga gatggcaagt ttttttgggt actattccaa aggaccagtg gatgcaaaga 1380
tggtgggaaa tgaagagaga tatggttggt gttgttgagc cattgccaag agatgaaact 1440
tactgtgatc caccagcttt gtttcatgtt tctggtgatt actctttcat ccgttacttt 1500
acccgtacca tttaccagtt tcagttccag aaggctttgt gcgaagctgc tggtcattct 1560
ggtccattgt ttaagtgtga tattaccaac tccaccgccg ccggtgataa gttgagaact 1620
atgttggaat ttggtcgttc caagtcctgg actagagctt tggaaactat ttccggtaac 1680
ccaaagatgg attctgctcc attgttggat tactttaagg acttgcacgt ctggttgttg 1740
gaagagaaca gaaagaacaa cagaaagcca ggttggaagg ctgctgaaga tccattttct 1800
gaaaacgcct acaaggttag attgtctttg aaggctgcta tgggtgataa ggcttacaag 1860
tggaacgcta acgaaatgta cttgtttaag gccaacatgg cctacgccat gagacaatac 1920
tacttggaag ttaacaagac cgccgctttg tttactactg agaacattca cacttacaag 1980
gagaccgcta gaatctcttt ttacttcgtt gtcactgacc cagctaactc cgctgttgtt 2040
attccaaagg ctgaagttga agctgctatt agaatgtcta gaggtagaat taacgacgcc 2100
tttaagttgg atgacaagac tttggagttc gaaggtttgt tggctacttt ggctccacca 2160
gttgaacaac cagttact 2178
<210> 36
<211> 1803
<212> DNA
<213> Artificial Sequence
<220>
<223> CsACE2-615（银鲑鱼）核苷酸序列
<400> 36
tccgatttgg aaagacgtgc tcaagaattt ttgaaccagt tcgatggtaa cgctacccat 60
ttgatgtacc aatactcttt ggcttcttgg gcttacaaca ctgatatttc tcaagagaac 120
ttggacaagt tgggtgtcca atctgctatt tggggtgaat actactctac tgtttctaag 180
gaatccgaga agttcccaat cgaccaaatc agagatccat tgattaagtt gcagttgatc 240
tccttgcaag acaagggttc tggtgctttg tctgctgata aggctgctca tttgaacaag 300
gttatgaacg aaatgtcctc catttactcc accggtactg tttgtaagcg tgaagatcca 360
tttgattgtc agactttgga accaggtttg gaatctgtta tggctaacat ggattctgac 420
tactacgaga gattgcacgt ctgggaaggt tggagagttg aagttggtaa gaagatgaga 480
ccattgtacg aagattacgt cgatttgaag aacgaggctg ctaagttgaa cgattacgaa 540
gattacggtg attactggag atccaactac gaaactactg acgattctcc ctacaactac 600
gctagaggtc aattgatgac tgatgttaga agaatctaca aggagatcct gcccttgtac 660
aaggagttgc acgcctacgt tagatccaag ttgcaggcta agcatccaga acatattcac 720
ccagaaggtg gtttgccagc tcatttgttg ggtgatatgt ggggtagatt ttggactggt 780
ttgtacccaa tttctacccc atttccagaa aagatcgata tcgacgttac taacgctatg 840
atcgcccaaa agtggccaaa ggatagattg tttcaagagg ctgaaaagtt cttcatgtcc 900
gtcggtttgt acaagatgtt tgacaacttt tggaaggact ccatgttgga aaagccaact 960
gatggtagaa aggttgtttg tcatccaact gcttgggata tgggtaacag agaagatttt 1020
agaatcaaga tgtgcaccga ggttaacatg gatcattttt tgaccgctca tcacgagatg 1080
ggtcataacc aataccaaat ggcttacaga aacttgtcct acttgttgcg tgacggtgct 1140
aacgaaggtt ttcatgaagc tgttggtgaa attatgtcct tgtccgctgc tactccaaag 1200
catttgaagg ctttgggttt gttgccagat gattttgttg aagacaagga gaccgaaatc 1260
aacttcttga tgaagcaggc cttgaccatt gttgctactt tgccatttac ttacatgttg 1320
gaggaatgga gatggcaagt ttttttgggt actattccaa aggaccagtg gatgcaaaga 1380
tggtgggaaa tgaagagaga tatggttggt gttgttgagc cattgccaag agatgaaact 1440
tactgtgatc caccagcttt gtttcatgtt tctggtgatt actctttcat ccgttacttt 1500
acccgtacca tttaccagtt tcagttccag aaggctttgt gcgaagctgc tggtcattct 1560
ggtccattgt ttaagtgtga tattaccaac tccaccgccg ccggtgataa gttgagaact 1620
atgttggaat ttggtcgttc caagtcctgg actagagctt tggaaactat ttccggtaac 1680
ccaaagatgg attctgctcc attgttggat tactttaagg acttgcacgt ctggttgttg 1740
gaagagaaca gaaagaacaa cagaaagcca ggttggaagg ctgctgaaga tccattttct 1800
gaa 1803
<210> 37
<211> 2178
<212> DNA
<213> Artificial Sequence
<220>
<223> RtACE2-740（虹鳟鱼）核苷酸序列
<400> 37
tccgatttgg aacgtagagc ccaagaattt ttggaccaat ttgacggtaa cgccactcat 60
ttgatgtacc aatactcttt ggcttcctgg gcttacaaca ctgatatttc tcaagagaac 120
ttggacaagt tgggtgttca atctactatc tggggtgaat actactccac tgtctctaag 180
gaatctgaaa agtttccaat cgaccagata tccgacccat tgatcagatt gcaattgatt 240
tccttgcagg acaagggttc tggtgctttg tctgctgata aggctgctca tttgaacaag 300
gttatgaacg aaatgtcctc catttactcc accggtaccg tctgtaagag agaagatcca 360
ttggattgtc aaaccttgga gccaggtttg gaatctgtta tggctaacat ggattctgac 420
tactacgaaa gattgcacgt ctgggaaggt tggagagttg aagttggtaa gaagatgaga 480
ccattgtacg aagattacgt cgatttgaag aacgaggccg ctaagttgaa cgattacgaa 540
gattacggtg attactggag atccaactac gaaactattg acgactctcc atacaactac 600
gctagaggtc aattgatgac tgatgttaga agaatctaca aggagatcct tccattgtac 660
aaggaattgc acgcctacgt tcgttctaag ttgcaagcta agcatccaga acatattcac 720
ccagaaggtg gtttgccagc tcatttgttg ggtgatatgt ggggtagatt ttggactggt 780
ttgtacccaa tttctacccc atttccagaa aagaccgata tcgatgttac tgaggctatg 840
attgcccaaa agtggccaaa ggatagattg tttcaagagg ccgaaaagtt cttcatgtct 900
gttggtttgt acaagatgtt tgacaacttc tggaaggact ctatgttgga gaagccaacc 960
gatggtagaa aggttgtttg tcatccaact gcttgggata tgggtaacag agaagatttt 1020
agaatcaaga tgtgcacgga ggtcaacatg gaccattttt tgaccgctca ccatgaaatg 1080
ggtcataacc aataccaaat ggcctacaga aacttgtctt acttgttgcg tgatggtgcc 1140
aacgaaggtt ttcatgaagc tgttggtgaa attatgtcct tgtctgctgc tactccaaag 1200
catttgaagg ctttgggttt gttgccaggt gattttgttg aagataagga gaccgaaatc 1260
aacttcttga tgaagcaggc tttgaccatt gttgctactt tgccatttac ttacatgttg 1320
gaggaatggc gttggcaagt ttttttgggt actattccaa aggaccagtg gatgcaaaga 1380
tggtgggaaa tgaagagaga tatggttggt gttgttgagc cattgccaag agatgaaact 1440
tactgtgatc caccagcttt gtttcatgtt tctggtgatt actctttcat ccgttacttt 1500
accagaaccg tctaccaatt tcaattccag aaggctttgt gcgaagccgc tggtcattct 1560
ggtccattgt ttaagtgtga tattaccaac tccaccgccg ctggtgataa gttgagaact 1620
atgttggaat ttggtcgttc caagtcctgg actcgtgctt tggaaactat ttctggtaac 1680
gctaagatgg actctgcccc attgttggat tactttaagg acttgcatgt ctggttgatc 1740
gaagagaaca gaaagaacaa cagaaagcca ggttggagag ctgctgaaga tccattttct 1800
gctaacgctt acaaggttag attgtccttg aaggctgcta tgggtgataa ggcttacatg 1860
tggaacgcta acgaaatgta cttgtttaag gccaacatgg cctacgctat gagacaatac 1920
tacttggaag ttaacaagac cgccgccttg tttaccactg aaaacattca tacctacaag 1980
gagactgcca gaatttcttt ttacttcgtc gtcaccgacc cagccaactc tgctgttgtt 2040
attccaaagg ctgaagttga agctgctatt agaatgtcta gaggtagaat taacgacgcc 2100
tttaagttgg atgataagac cttggaattt gagggtttgt tggccacttt ggccccacca 2160
gttgaacaac cagttact 2178
<210> 38
<211> 1803
<212> DNA
<213> Artificial Sequence
<220>
<223> RtACE2-615（虹鳟鱼）核苷酸序列
<400> 38
tccgatttgg aacgtagagc ccaagaattt ttggaccaat ttgacggtaa cgccactcat 60
ttgatgtacc aatactcttt ggcttcctgg gcttacaaca ctgatatttc tcaagagaac 120
ttggacaagt tgggtgttca atctactatc tggggtgaat actactccac tgtctctaag 180
gaatctgaaa agtttccaat cgaccagata tccgacccat tgatcagatt gcaattgatt 240
tccttgcagg acaagggttc tggtgctttg tctgctgata aggctgctca tttgaacaag 300
gttatgaacg aaatgtcctc catttactcc accggtaccg tctgtaagag agaagatcca 360
ttggattgtc aaaccttgga gccaggtttg gaatctgtta tggctaacat ggattctgac 420
tactacgaaa gattgcacgt ctgggaaggt tggagagttg aagttggtaa gaagatgaga 480
ccattgtacg aagattacgt cgatttgaag aacgaggccg ctaagttgaa cgattacgaa 540
gattacggtg attactggag atccaactac gaaactattg acgactctcc atacaactac 600
gctagaggtc aattgatgac tgatgttaga agaatctaca aggagatcct tccattgtac 660
aaggaattgc acgcctacgt tcgttctaag ttgcaagcta agcatccaga acatattcac 720
ccagaaggtg gtttgccagc tcatttgttg ggtgatatgt ggggtagatt ttggactggt 780
ttgtacccaa tttctacccc atttccagaa aagaccgata tcgatgttac tgaggctatg 840
attgcccaaa agtggccaaa ggatagattg tttcaagagg ccgaaaagtt cttcatgtct 900
gttggtttgt acaagatgtt tgacaacttc tggaaggact ctatgttgga gaagccaacc 960
gatggtagaa aggttgtttg tcatccaact gcttgggata tgggtaacag agaagatttt 1020
agaatcaaga tgtgcacgga ggtcaacatg gaccattttt tgaccgctca ccatgaaatg 1080
ggtcataacc aataccaaat ggcctacaga aacttgtctt acttgttgcg tgatggtgcc 1140
aacgaaggtt ttcatgaagc tgttggtgaa attatgtcct tgtctgctgc tactccaaag 1200
catttgaagg ctttgggttt gttgccaggt gattttgttg aagataagga gaccgaaatc 1260
aacttcttga tgaagcaggc tttgaccatt gttgctactt tgccatttac ttacatgttg 1320
gaggaatggc gttggcaagt ttttttgggt actattccaa aggaccagtg gatgcaaaga 1380
tggtgggaaa tgaagagaga tatggttggt gttgttgagc cattgccaag agatgaaact 1440
tactgtgatc caccagcttt gtttcatgtt tctggtgatt actctttcat ccgttacttt 1500
accagaaccg tctaccaatt tcaattccag aaggctttgt gcgaagccgc tggtcattct 1560
ggtccattgt ttaagtgtga tattaccaac tccaccgccg ctggtgataa gttgagaact 1620
atgttggaat ttggtcgttc caagtcctgg actcgtgctt tggaaactat ttctggtaac 1680
gctaagatgg actctgcccc attgttggat tactttaagg acttgcatgt ctggttgatc 1740
gaagagaaca gaaagaacaa cagaaagcca ggttggagag ctgctgaaga tccattttct 1800
gct 1803
<210> 39
<211> 2064
<212> DNA
<213> Artificial Sequence
<220>
<223> SalACE2-740（三文鱼）核苷酸序列
<400> 39
atgaacaaga tgtcctctat ttactccacc ggtactgttt gtaagagaga agatccattt 60
gactgccaga ctttggagcc aggtttggaa tctgttatgg ctaacatgga ttctgactac 120
tacgaacgtt tgcacgtctg ggagggttgg agagttgaag ttggtaagaa gatgagacca 180
ttgtacgaag attacgtcga tttgaagaac gaggctgcta agttgaacgg ttacgaagat 240
tacggtgatt actggagatc caactacgaa actattgacg actctcccta caactacgcc 300
agaggtcaat tgatgactga tgttagacat atctacaagg aaatcttgcc cttgtacaag 360
gagttgcatg cctacgttag atccaagttg caggctaagc atccagaaca tattcatcca 420
gaaggtggtt tgccagctca tttgttgggt gatatgtggg gtagattttg gactggtttg 480
tacccaattt ctaccccatt tccagaaaag actgatatcg atgttaccga cgctatgatt 540
gcccaaaagt ggccaaagga tagattgttt caagaggctg aaaagttctt catgtccgtc 600
ggtttgtaca agatgtttga taacttttgg aaggactcca tgttggagaa gccaactgat 660
ggtagaaagg ttgtttgtca tccaactgct tgggatatgg gtaacagaga agattttaga 720
atcaagatgt gcactgaggt caacatggac cattttttga ccgcccatca tgaaatgggt 780
cacaaccaat accaaatggc ttacagaaac ttgtcctact tgttgcgtga tggtgctaac 840
gaaggttttc atgaagctgt tggtgaaatt atgagcttgt ccgctgctac tccaaagcat 900
ttgaaggctt tgggtttgtt gccagatgat tttgttgaag acaaggagac cgaaatcaac 960
ttcttgatga agcaggcttt gaccattgtc gccactttgc catttactta catgttggag 1020
gagtggagat ggcaagtttt tttgggtact attccaaagg accagtggat gcaaagatgg 1080
tgggaaatga agagagatat ggttggtgtt gttgagccat tgccaagaga tgaaacttac 1140
tgtgatccac cagctttgtt tcatgtttct ggtgattact ctttcatccg ctactttacc 1200
agaaccattt accagttcca attccagaag gctttgtgtg aggctgctgg tcattctggt 1260
ccattgttta agtgtgatat taccaactcc accgccgctg gtgataagtt gagaactatg 1320
ttggaatttg gtcgttccaa gtcctggact agagctttgg aaactatttc cggtcatgct 1380
aagatggatt ctgctccatt gttggattac tttaaggact tgcacgtctg gttgattgaa 1440
gagaacagaa agaacaaccg taagccaggt tggagagctg ctgaagatcc attttctgaa 1500
aacgcttaca aggtccgttt gtccttgaag gccgctatgg gtgataaggc ttacatttgg 1560
aacgctaacg aaatgtactt gttcaaggct aacatggcct acgctatgag acaatactac 1620
ttggaagtta acaagaccga ggttttgttc accactgaga acattcacac ctacaaggag 1680
accgctagaa tttcctttta ctttgtcgtt accgacccag ccaacccagc tgttgttatt 1740
ccaaaggctg aagttgaagc tgctattaga ttgtctagag gtagaattaa cgacgccttt 1800
aagttggacg ataagacctt ggaatttgag ggtttgttgg ctactttggc cccaccagtt 1860
gaacaaccag ttactgtttg gttggttgtt tttggtgttg tcatgggttt ggtcgtttgc 1920
atgggttgtt acttgattat ctctggtttt cgtgaccgta agaagaagtg cgccgctaag 1980
gctaaggaaa acgctgaaaa cccatacggt gttactaaca agacttttga gagagaggaa 2040
gacgaacaga ccggttttca tcac 2064
<210> 40
<211> 1875
<212> DNA
<213> Artificial Sequence
<220>
<223> SalACE2-615（三文鱼）核苷酸序列
<400> 40
atgaacaaga tgtcctctat ttactccacc ggtactgttt gtaagagaga agatccattt 60
gactgccaga ctttggagcc aggtttggaa tctgttatgg ctaacatgga ttctgactac 120
tacgaacgtt tgcacgtctg ggagggttgg agagttgaag ttggtaagaa gatgagacca 180
ttgtacgaag attacgtcga tttgaagaac gaggctgcta agttgaacgg ttacgaagat 240
tacggtgatt actggagatc caactacgaa actattgacg actctcccta caactacgcc 300
agaggtcaat tgatgactga tgttagacat atctacaagg aaatcttgcc cttgtacaag 360
gagttgcatg cctacgttag atccaagttg caggctaagc atccagaaca tattcatcca 420
gaaggtggtt tgccagctca tttgttgggt gatatgtggg gtagattttg gactggtttg 480
tacccaattt ctaccccatt tccagaaaag actgatatcg atgttaccga cgctatgatt 540
gcccaaaagt ggccaaagga tagattgttt caagaggctg aaaagttctt catgtccgtc 600
ggtttgtaca agatgtttga taacttttgg aaggactcca tgttggagaa gccaactgat 660
ggtagaaagg ttgtttgtca tccaactgct tgggatatgg gtaacagaga agattttaga 720
atcaagatgt gcactgaggt caacatggac cattttttga ccgcccatca tgaaatgggt 780
cacaaccaat accaaatggc ttacagaaac ttgtcctact tgttgcgtga tggtgctaac 840
gaaggttttc atgaagctgt tggtgaaatt atgagcttgt ccgctgctac tccaaagcat 900
ttgaaggctt tgggtttgtt gccagatgat tttgttgaag acaaggagac cgaaatcaac 960
ttcttgatga agcaggcttt gaccattgtc gccactttgc catttactta catgttggag 1020
gagtggagat ggcaagtttt tttgggtact attccaaagg accagtggat gcaaagatgg 1080
tgggaaatga agagagatat ggttggtgtt gttgagccat tgccaagaga tgaaacttac 1140
tgtgatccac cagctttgtt tcatgtttct ggtgattact ctttcatccg ctactttacc 1200
agaaccattt accagttcca attccagaag gctttgtgtg aggctgctgg tcattctggt 1260
ccattgttta agtgtgatat taccaactcc accgccgctg gtgataagtt gagaactatg 1320
ttggaatttg gtcgttccaa gtcctggact agagctttgg aaactatttc cggtcatgct 1380
aagatggatt ctgctccatt gttggattac tttaaggact tgcacgtctg gttgattgaa 1440
gagaacagaa agaacaaccg taagccaggt tggagagctg ctgaagatcc attttctgaa 1500
aacgcttaca aggtccgttt gtccttgaag gccgctatgg gtgataaggc ttacatttgg 1560
aacgctaacg aaatgtactt gttcaaggct aacatggcct acgctatgag acaatactac 1620
ttggaagtta acaagaccga ggttttgttc accactgaga acattcacac ctacaaggag 1680
accgctagaa tttcctttta ctttgtcgtt accgacccag ccaacccagc tgttgttatt 1740
ccaaaggctg aagttgaagc tgctattaga ttgtctagag gtagaattaa cgacgccttt 1800
aagttggacg ataagacctt ggaatttgag ggtttgttgg ctactttggc cccaccagtt 1860
gaacaaccag ttact 1875
<210> 41
<211> 2178
<212> DNA
<213> Artificial Sequence
<220>
<223> StACE2-740（亚东鲑）核苷酸序列
<400> 41
tctgacttgg aaagaagagc ccaagaattt ttggatacct ttgacggtaa cgccacccat 60
ttgatgtacc aatactcttt ggcttcttgg gcttacaaca ctgatatttc tcaagagaac 120
ttggacaagt tgggtgttca atccgctatt tggggtgaat actactctaa ggtttctaag 180
gaatccgaga acttcccaat tgaccaaatt tctgatccat tgatcaagtt gcagttgacg 240
tccttgcagg acaagggttc tggtgctttg tctgctgata aggctgctca tttgaacaag 300
gttatgaaca agatgtcctc catctactcc accggtactg tctgtaagag agaagatcca 360
tttgattgcc agaccttgga gccaggtttg gaatctgtta tggctaacat ggattctgac 420
tactacgaaa gattgcacgt ttgggaaggt tggagagttg aagttggtaa gaagatgaga 480
ccattgtacg aagattacgt cgatttgaag aacgaggccg ctaagttgaa cggttacgaa 540
gattacggtg attactggag atccaactac gaaactattg acgactcccc atacaactac 600
gccagaggtc aattgatgac tgatgttaga agaatctaca aggagatatt gcccttgtac 660
aaggagttgc atgcttacgt tagatccaag ttgcaagcca agcatccaga acatattcac 720
ccagaaggtg gtttgccagc tcatttgttg ggtgatatgt ggggtagatt ttggactggt 780
ttgtacccaa tttctacccc atttccagaa aagactgata tcgatgttac cgacgccatg 840
atcgctcaaa agtggccaaa ggatagattg tttcaagagg ctgaaaagtt ctttatgtcc 900
gtcggtttgt acaagatgtt cgataacttt tggaaggact ccatgttgga gaagccaact 960
gatggtagaa aggttgtttg tcatccaact gcttgggata tgggtaacag agaagatttt 1020
agaatcaaga tgtgcaccga ggtcaacatg gatcactttt tgactgccca ccatgagatg 1080
ggtcataacc aataccaaat ggcttacaga aacttgtcct acttgttgag agatggtgct 1140
aacgaaggtt ttcatgaagc tgttggtgaa attatgtcct tgtctgccgc tactccaaag 1200
catttgaagg ctttgggttt gttgccagat gattttgttg aagacaagga gaccgagatc 1260
aactttttga tgaagcaggc cttgactatt gtcgccactt tgccatttac ttacatgttg 1320
gaggaatgga gatggcaagt ttttttgggt actattccaa aggaccagtg gatgcaaaga 1380
tggtgggaaa tgaagagaga tatggttggt gttgttgagc cattgccaag agatgaaact 1440
tactgtgatc caccagcttt gtttcatgtt tctggtgatt actctttcat ccgttacttc 1500
actcgtacta tctaccagtt tcaattccag aaggctttgt gtgaagccgc tggtcattct 1560
ggtccattgt ttaagtgtga tattaccaac tccaccgccg ccggtgataa gttgagaact 1620
atgttggaat ttggtcgttc caagtcctgg actagagctt tggaaactat ttccggtcat 1680
gctaagatgg attccgcccc attgttggat tactttaagg atttgcatgt ctggttgatc 1740
gaggagaacc gtaagaacaa cagaaagcca ggttggagag ctgctgaaga tccattttct 1800
gaaaacgctt acaaggtcag attgtctttg aaggctgcta tgggtgataa ggcttacatt 1860
tggaacggta acgaaatgta cttgttcaag gctaacatgg cctacgctat gagacaatac 1920
tacttggaag ttaacaagac cgaggttttg ttcaccactg agaacatcca tacttacaag 1980
gagactgcta gaatttcctt ctacttcgtc gttactgatc cagccaaccc agctgttgtt 2040
attccaaagg ctgaagttga agctgctatt agattgtcta gaggcagaat taacgacgcc 2100
tttaagttgg acgataagac tttggagttc gagggtttgt tggccacttt ggctccacca 2160
gttgaacaac cagttact 2178
<210> 42
<211> 1803
<212> DNA
<213> Artificial Sequence
<220>
<223> StACE2-615（亚东鲑）核苷酸序列
<400> 42
tctgacttgg aaagaagagc ccaagaattt ttggatacct ttgacggtaa cgccacccat 60
ttgatgtacc aatactcttt ggcttcttgg gcttacaaca ctgatatttc tcaagagaac 120
ttggacaagt tgggtgttca atccgctatt tggggtgaat actactctaa ggtttctaag 180
gaatccgaga acttcccaat tgaccaaatt tctgatccat tgatcaagtt gcagttgacg 240
tccttgcagg acaagggttc tggtgctttg tctgctgata aggctgctca tttgaacaag 300
gttatgaaca agatgtcctc catctactcc accggtactg tctgtaagag agaagatcca 360
tttgattgcc agaccttgga gccaggtttg gaatctgtta tggctaacat ggattctgac 420
tactacgaaa gattgcacgt ttgggaaggt tggagagttg aagttggtaa gaagatgaga 480
ccattgtacg aagattacgt cgatttgaag aacgaggccg ctaagttgaa cggttacgaa 540
gattacggtg attactggag atccaactac gaaactattg acgactcccc atacaactac 600
gccagaggtc aattgatgac tgatgttaga agaatctaca aggagatatt gcccttgtac 660
aaggagttgc atgcttacgt tagatccaag ttgcaagcca agcatccaga acatattcac 720
ccagaaggtg gtttgccagc tcatttgttg ggtgatatgt ggggtagatt ttggactggt 780
ttgtacccaa tttctacccc atttccagaa aagactgata tcgatgttac cgacgccatg 840
atcgctcaaa agtggccaaa ggatagattg tttcaagagg ctgaaaagtt ctttatgtcc 900
gtcggtttgt acaagatgtt cgataacttt tggaaggact ccatgttgga gaagccaact 960
gatggtagaa aggttgtttg tcatccaact gcttgggata tgggtaacag agaagatttt 1020
agaatcaaga tgtgcaccga ggtcaacatg gatcactttt tgactgccca ccatgagatg 1080
ggtcataacc aataccaaat ggcttacaga aacttgtcct acttgttgag agatggtgct 1140
aacgaaggtt ttcatgaagc tgttggtgaa attatgtcct tgtctgccgc tactccaaag 1200
catttgaagg ctttgggttt gttgccagat gattttgttg aagacaagga gaccgagatc 1260
aactttttga tgaagcaggc cttgactatt gtcgccactt tgccatttac ttacatgttg 1320
gaggaatgga gatggcaagt ttttttgggt actattccaa aggaccagtg gatgcaaaga 1380
tggtgggaaa tgaagagaga tatggttggt gttgttgagc cattgccaag agatgaaact 1440
tactgtgatc caccagcttt gtttcatgtt tctggtgatt actctttcat ccgttacttc 1500
actcgtacta tctaccagtt tcaattccag aaggctttgt gtgaagccgc tggtcattct 1560
ggtccattgt ttaagtgtga tattaccaac tccaccgccg ccggtgataa gttgagaact 1620
atgttggaat ttggtcgttc caagtcctgg actagagctt tggaaactat ttccggtcat 1680
gctaagatgg attccgcccc attgttggat tactttaagg atttgcatgt ctggttgatc 1740
gaggagaacc gtaagaacaa cagaaagcca ggttggagag ctgctgaaga tccattttct 1800
gaa 1803
<210> 43
<211> 2169
<212> DNA
<213> Artificial Sequence
<220>
<223> MlACE2-740（水貂）核苷酸序列
<400> 43
cagtctacta ccgaagattt ggctaagact ttcttggaaa agttcaacta cgaggccgaa 60
gaattgtctt accaaaactc tttggcttcc tggaactaca acactaacat tactgatgag 120
aacatccaga agatgaacat cgccggtgcc aagtggtctg ctttttacga agaagaatct 180
cagcatgcca agacctaccc attggaagaa attcaggacc caattattaa gcgtcagttg 240
agagccttgc aacagtctgg ttcttctgtt ttgtctgctg ataagagaga acgcttgaac 300
actattttga acgccatgtc cactatctac tccactggta aggcttgtaa cccaaacaac 360
ccacaagaat gtttgttgtt ggaaccaggt ttggatgata ttatggaaaa ctccaaggac 420
tacaacgagc gtttgtgggc ttgggaaggt tggcgttctg aagttggtaa gcaattgaga 480
ccattgtacg aagaatacgt cgctttgaag aacgaaatgg ccagagctaa caactacgaa 540
gattacggtg attactggag aggtgattac gaagaagaat gggctgatgg ttactcttac 600
tctagaaacc aattgatcga ggacgtcgag catactttta ctcaaatcaa gccattgtac 660
gagcacttgc acgcttacgt tagagctaag ttgatggatg cttacccatc tagaatttcc 720
ccaactggtt gtttgccagc tcatttgttg ggtgatatgt ggggtagatt ttggactaac 780
ttgtacccat tgatggtccc atttggtcag aagccaaaca ttgacgttac tgacgctatg 840
gttaaccaat cttgggatgc tagaagaatt ttcgaggagg ctgaaacctt ttttgtttcc 900
gttggtttgc caaacatgac cgaaggtttt tggcaaaact ctatgttgac tgagccaggt 960
gataacagaa aggttgtttg tcatccaact gcctgggatt tgggtaagag agattttaga 1020
attaagatgt gcaccaaggt caccatggac gacttcttga ctgctcatca tgaaatgggt 1080
catattcaat acgacatggc ctacgctgaa caaccatttt tgttgagaaa cggtgctaac 1140
gaaggttttc atgaagctgt tggtgaaatt atgtccttgt ctgccgctac tccaaaccat 1200
ttgaagaaca ttggtttgtt gcccccagat ttttccgaag actctgaaac tgacattaac 1260
ttcttgttga agcaagcctt gaccatcgtt ggtactttgc catttactta catgttggag 1320
aagtggcgtt ggatggtttt taagggtgaa attccaaagg agcagtggat gcaaaagtgg 1380
tgggaaatga agagagatat tgtcggtgtt gttgagccat tgccacatga tgaaacttac 1440
tgtgatccag ctgctttgtt tcatgttgct aacgattact ctttcatccg ttactacacc 1500
cgtactatct accagtttca atttcaggaa gccttgtgtc aaattgccaa gcacgaaggt 1560
ccattgtaca agtgtgatat ttctaactcc agagaggccg gtcaaaagtt gcatgaaatg 1620
ttgtctttgg gtcgttctaa gccatggact tttgctttgg aaagagttgt tggtgctaag 1680
actatggatg ttagaccatt gttgaactac ttcgagccat tgtttacttg gttgaaggag 1740
cagaacagaa actccttcgt cggttggaac actgattggt ctccatacgc tgatcaatcc 1800
attaaggtcc gtatctcttt gaagtctgct ttgggtgaaa aggcttacga atggaacgat 1860
aacgaaatgt actttttcca gtcctccatc gcttacgcta tgagagaata cttttccaag 1920
gtcaagaacc agactattcc atttgttggt aaggacgtta gagtctccga tttgaagcca 1980
agaatttcct ttaacttcat cgtcacctcc ccagagaaca tgtctgatat tattccaaga 2040
gccgatgtcg aagaggccat tcgtaagtct agaggtagaa ttaacgatgc ctttcgtttg 2100
gacgataact ccttggaatt tttgggtatc cagccaacct tggagccacc ataccaacca 2160
ccagttact 2169
<210> 44
<211> 1794
<212> DNA
<213> Artificial Sequence
<220>
<223> MlACE2-615（水貂）核苷酸序列
<400> 44
cagtctacta ccgaagattt ggctaagact ttcttggaaa agttcaacta cgaggccgaa 60
gaattgtctt accaaaactc tttggcttcc tggaactaca acactaacat tactgatgag 120
aacatccaga agatgaacat cgccggtgcc aagtggtctg ctttttacga agaagaatct 180
cagcatgcca agacctaccc attggaagaa attcaggacc caattattaa gcgtcagttg 240
agagccttgc aacagtctgg ttcttctgtt ttgtctgctg ataagagaga acgcttgaac 300
actattttga acgccatgtc cactatctac tccactggta aggcttgtaa cccaaacaac 360
ccacaagaat gtttgttgtt ggaaccaggt ttggatgata ttatggaaaa ctccaaggac 420
tacaacgagc gtttgtgggc ttgggaaggt tggcgttctg aagttggtaa gcaattgaga 480
ccattgtacg aagaatacgt cgctttgaag aacgaaatgg ccagagctaa caactacgaa 540
gattacggtg attactggag aggtgattac gaagaagaat gggctgatgg ttactcttac 600
tctagaaacc aattgatcga ggacgtcgag catactttta ctcaaatcaa gccattgtac 660
gagcacttgc acgcttacgt tagagctaag ttgatggatg cttacccatc tagaatttcc 720
ccaactggtt gtttgccagc tcatttgttg ggtgatatgt ggggtagatt ttggactaac 780
ttgtacccat tgatggtccc atttggtcag aagccaaaca ttgacgttac tgacgctatg 840
gttaaccaat cttgggatgc tagaagaatt ttcgaggagg ctgaaacctt ttttgtttcc 900
gttggtttgc caaacatgac cgaaggtttt tggcaaaact ctatgttgac tgagccaggt 960
gataacagaa aggttgtttg tcatccaact gcctgggatt tgggtaagag agattttaga 1020
attaagatgt gcaccaaggt caccatggac gacttcttga ctgctcatca tgaaatgggt 1080
catattcaat acgacatggc ctacgctgaa caaccatttt tgttgagaaa cggtgctaac 1140
gaaggttttc atgaagctgt tggtgaaatt atgtccttgt ctgccgctac tccaaaccat 1200
ttgaagaaca ttggtttgtt gcccccagat ttttccgaag actctgaaac tgacattaac 1260
ttcttgttga agcaagcctt gaccatcgtt ggtactttgc catttactta catgttggag 1320
aagtggcgtt ggatggtttt taagggtgaa attccaaagg agcagtggat gcaaaagtgg 1380
tgggaaatga agagagatat tgtcggtgtt gttgagccat tgccacatga tgaaacttac 1440
tgtgatccag ctgctttgtt tcatgttgct aacgattact ctttcatccg ttactacacc 1500
cgtactatct accagtttca atttcaggaa gccttgtgtc aaattgccaa gcacgaaggt 1560
ccattgtaca agtgtgatat ttctaactcc agagaggccg gtcaaaagtt gcatgaaatg 1620
ttgtctttgg gtcgttctaa gccatggact tttgctttgg aaagagttgt tggtgctaag 1680
actatggatg ttagaccatt gttgaactac ttcgagccat tgtttacttg gttgaaggag 1740
cagaacagaa actccttcgt cggttggaac actgattggt ctccatacgc tgat 1794
<210> 45
<211> 2169
<212> DNA
<213> Artificial Sequence
<220>
<223> VvACE2-740（狐狸）核苷酸序列
<400> 45
cagtcaacag aagatttagt gaatacgttt ctcgagaagt ttaattacga ggctgaagag 60
ttatcgtatc agagttcttt ggccagttgg gactataata cgaatatttc cgacgaaaac 120
gtacagaaaa tgaacaatgc cggagcaaag tggtcggcat tctatgaaga gcagagtaaa 180
ctcgccaaaa cttacccgct cgaagagata caagattcta cagtgaagcg tcaactaaga 240
gcattacaac attcaggttc ttctgttcta tctgctgaca agaaccaaag attaaatacc 300
attttgaact ctatgtccac tatatattcc actggaaaag catgtaatcc ttcgaacccg 360
caagagtgtt tactactgga gcccggcctc gatgatatta tggagaacag caaagattac 420
aacgagcgcc tttgggcttg ggaggggtgg cggtcagaag taggaaaaca gctaaggcca 480
ctctacgagg agtacgtcgc acttaagaat gaaatggcca gggcgaacaa ttatgaggac 540
tatggagact actggcgtgg ggattatgaa gaggagtggg agaacgggta taactacagt 600
cgcaatcagc taatagatga cgtggagcac actttcaccc aaatcatgcc cctgtaccag 660
cacttacacg catacgttcg cacgaagcta atggatacgt acccgtccta tatatcgcct 720
accgggtgct tgcccgccca cctgcttggt gatatgtggg gtcgtttttg gactaatttg 780
tatcccctca cggtaccttt tggtcagaaa ccgaacattg atgtcactaa cgcgatggtc 840
aaccagtcgt gggatgcgag aaaaatcttc aaagaagcgg aaaagttctt cgtaagcgtt 900
gggctgccaa acatgactca gggcttttgg gaaaacagca tgcttactga accctccgac 960
tcgcgtaagg tcgtgtgcca tccgacagct tgggaccttg gaaaaggaga ttttcgaatt 1020
aaaatgtgca caaaagtcac catggatgac ttcctcacgg cacaccatga gatggggcac 1080
atacaatacg atatggcata cgccgctcag ccattcttgc tgcgaaatgg cgccaatgaa 1140
ggtttccatg aagcggtcgg cgaaatcatg agccttagtg ctgccacacc aaaccaccta 1200
aaaaatatcg gcctattacc tccttcgttt tttgaagata gtgaaacgga aataaatttc 1260
ctgttaaaac aggcacttac aatcgtaggc acactgcctt tcacctatat gttagaaaaa 1320
tggcggtgga tggtgtttaa aggtgaaatc ccgaaggacc aatggatgaa gacttggtgg 1380
gagatgaagc gcaatattgt gggagtagtg gagccagtcc ctcatgacga aacatattgt 1440
gacccggcca gcctttttca tgttgctaac gactattcct ttatccgata ctatacgagg 1500
accatttacc aattccaatt ccaggaagcg ttgtgccaaa tagctaagca cgagggacca 1560
cttcacaagt gtgacatttc taattccagt gaggctgggc aaaagctact ggaaatgcta 1620
aaactgggta agtcaaagcc ttggacgtat gccttggaaa tcgtcgtagg ggccaaaaat 1680
atggacgtgc gaccgctgct aaactacttt gaaccattgt ttacttggtt gaaggagcaa 1740
aacagaaatt cctttgttgg ctggaataca gactggagcc cctatgcaga tcagtcgatc 1800
aaggtaagaa taagtctgaa gagcgcgttg ggcgaaaaag cttatgaatg gaataataac 1860
gagatgtacc ttttccggtc gtctattgcg tacgcgatgc gacgatactt ttcagaggtg 1920
aagaaacaga ccatcccctt tgttgaggac aacgtttggg tttctgacct taaaccgagg 1980
atatcattta atttctttgt cacctcacca gggaacgttt cagacattat tccgcggaca 2040
gaagtagaga aggcgatacg gatgtatcgt ggtcgcataa atgatgtgtt caggttagat 2100
gataactctc tcgaattttt aggcatacaa cccaccttgg gtcctagtta cgagccaccc 2160
gttaccatc 2169
<210> 46
<211> 1791
<212> DNA
<213> Artificial Sequence
<220>
<223> VvACE2-615（狐狸）核苷酸序列
<400> 46
cagtcaacag aagatttagt gaatacgttt ctcgagaagt ttaattacga ggctgaagag 60
ttatcgtatc agagttcttt ggccagttgg gactataata cgaatatttc cgacgaaaac 120
gtacagaaaa tgaacaatgc cggagcaaag tggtcggcat tctatgaaga gcagagtaaa 180
ctcgccaaaa cttacccgct cgaagagata caagattcta cagtgaagcg tcaactaaga 240
gcattacaac attcaggttc ttctgttcta tctgctgaca agaaccaaag attaaatacc 300
attttgaact ctatgtccac tatatattcc actggaaaag catgtaatcc ttcgaacccg 360
caagagtgtt tactactgga gcccggcctc gatgatatta tggagaacag caaagattac 420
aacgagcgcc tttgggcttg ggaggggtgg cggtcagaag taggaaaaca gctaaggcca 480
ctctacgagg agtacgtcgc acttaagaat gaaatggcca gggcgaacaa ttatgaggac 540
tatggagact actggcgtgg ggattatgaa gaggagtggg agaacgggta taactacagt 600
cgcaatcagc taatagatga cgtggagcac actttcaccc aaatcatgcc cctgtaccag 660
cacttacacg catacgttcg cacgaagcta atggatacgt acccgtccta tatatcgcct 720
accgggtgct tgcccgccca cctgcttggt gatatgtggg gtcgtttttg gactaatttg 780
tatcccctca cggtaccttt tggtcagaaa ccgaacattg atgtcactaa cgcgatggtc 840
aaccagtcgt gggatgcgag aaaaatcttc aaagaagcgg aaaagttctt cgtaagcgtt 900
gggctgccaa acatgactca gggcttttgg gaaaacagca tgcttactga accctccgac 960
tcgcgtaagg tcgtgtgcca tccgacagct tgggaccttg gaaaaggaga ttttcgaatt 1020
aaaatgtgca caaaagtcac catggatgac ttcctcacgg cacaccatga gatggggcac 1080
atacaatacg atatggcata cgccgctcag ccattcttgc tgcgaaatgg cgccaatgaa 1140
ggtttccatg aagcggtcgg cgaaatcatg agccttagtg ctgccacacc aaaccaccta 1200
aaaaatatcg gcctattacc tccttcgttt tttgaagata gtgaaacgga aataaatttc 1260
ctgttaaaac aggcacttac aatcgtaggc acactgcctt tcacctatat gttagaaaaa 1320
tggcggtgga tggtgtttaa aggtgaaatc ccgaaggacc aatggatgaa gacttggtgg 1380
gagatgaagc gcaatattgt gggagtagtg gagccagtcc ctcatgacga aacatattgt 1440
gacccggcca gcctttttca tgttgctaac gactattcct ttatccgata ctatacgagg 1500
accatttacc aattccaatt ccaggaagcg ttgtgccaaa tagctaagca cgagggacca 1560
cttcacaagt gtgacatttc taattccagt gaggctgggc aaaagctact ggaaatgcta 1620
aaactgggta agtcaaagcc ttggacgtat gccttggaaa tcgtcgtagg ggccaaaaat 1680
atggacgtgc gaccgctgct aaactacttt gaaccattgt ttacttggtt gaaggagcaa 1740
aacagaaatt cctttgttgg ctggaataca gactggagcc cctatgcaga t 1791
<210> 47
<211> 2172
<212> DNA
<213> Artificial Sequence
<220>
<223> EcACE2-740（马）核苷酸序列
<400> 47
cagtccacta ctgaggacct agcaaagacg ttccttgaaa agttcaatag tgaggcggaa 60
gagttgtcac accagtcttc attagcatcc tggtcgtaca acacaaacat caccgatgaa 120
aacgttcaaa agatgaatga agcgggagcg agatggtctg ctttttacga ggagcaatgt 180
aagctggcca aaacctaccc attggaggaa atacaaaacc tcacagttaa acgtcaacta 240
caagccttgc aacaaagtgg ttcttcagta ctttccgccg ataaaagcaa gcgactaaac 300
gagatattaa atactatgtc cacaatttac tccacaggaa aggtctgcaa ccctagcaat 360
ccgcaggaat gtctacttct ggagccgggg ctggacgcaa taatggaaaa ctccaaagac 420
tataaccaga ggctatgggc ctgggaagga tggcggtcag aggtaggcaa acaactccgc 480
ccgttgtacg aagagtacgt tgtgcttaaa aatgaaatgg cacgagcaaa caattatgaa 540
gattacgggg attattggcg tggagattac gaggcagagg gcccgagcgg ttacgattac 600
tcacgggatc agctgatcga agacgtagaa cgaacgttcg ctgaaatcaa gccactctac 660
gagcacttac atgcgtatgt tagagcgaag ttgatggaca catatccatc tcacatcaac 720
ccaaccggtt gccttccggc ccatttattg ggtgacatgt ggggcagatt ttggactaac 780
ttgtatagct taacggtacc cttcggtcag aaacccaata ttgatgtgac ggatgcaatg 840
gttgatcaaa gctgggacgc taaaaggatt ttcgaagaag ctgagaagtt cttcgtgtcg 900
gtcgggctcc caaatatgac tcaagggttt tgggagaata gcatgttgac ggagcctggc 960
gacggccgga aagtcgtttg ccaccctacc gcatgggacc tagggaaagg agatttccga 1020
attaagatgt gcactaaggt caccatggac gatttcctca cagctcatca tgagatgggc 1080
cacattcagt atgacatggc ctatgcagta cagccctacc tactgcgcaa cggtgcaaat 1140
gagggctttc acgaggccgt tggcgaaata atgtcattga gcgcggccac ccccaatcat 1200
ctaaaggcca ttggactttt acctcctgat ttctacgaag attctgaaac tgagattaac 1260
ttcctcttaa aacaggcttt aacgatagtg ggaacgctac catttacata tatgctggaa 1320
aagtggagat ggatggtctt taaaggtgaa attcctaaag aggagtggat gaagaaatgg 1380
tgggagatga agcgtgagat tgtgggggtg gttgagccag taccacatga cgaaacatac 1440
tgtgatccag cagccttgtt tcacgtcgcg aatgactact cgtttatacg ttattatacg 1500
cgcactatct atcaattcca atttcaggaa gcgctgtgcc agactgctaa acacgaagga 1560
ccgcttcaca agtgtgacat cagcaattcc accgaagctg gtcagaagtt gcttcaaatg 1620
ctctcgttag gaaaatccga accctggacc ttagcgctcg agcgcatcgt gggggtgaaa 1680
aacatggatg ttcggccgtt acttaactat tttgagcccc tgttcacctg gctgaaagat 1740
cagaataaaa acagtttcgt gggctggagt acaaattggt ctccctacgc tgatcaatct 1800
atcaaagtac ggatatcgct aaagagtgcg ctgggtgaaa agagttatga atggaatgat 1860
aacgagatgt acctatttca gtccagtgtt gcctatgcta tgagggtcta cttccttaaa 1920
gcgaagaatc aaactatact gtttggcgag gaagacgtct gggtctctga tttaaagccg 1980
cgaatatcgt ttaatttctt tgtaacatcg ccgaagaacg catctgacat aatacccagg 2040
accgacgtag aagaggcgat ccgtatgagt aggtctcgca ttaacgacgc ttttagatta 2100
gacgataata cgctcgagtt tttaggtatt caacctactc ttgggcctcc ttatcagccc 2160
cctgtaacgg tt 2172
<210> 48
<211> 1794
<212> DNA
<213> Artificial Sequence
<220>
<223> EcACE2-615（马）核苷酸序列
<400> 48
cagtccacta ctgaggacct agcaaagacg ttccttgaaa agttcaatag tgaggcggaa 60
gagttgtcac accagtcttc attagcatcc tggtcgtaca acacaaacat caccgatgaa 120
aacgttcaaa agatgaatga agcgggagcg agatggtctg ctttttacga ggagcaatgt 180
aagctggcca aaacctaccc attggaggaa atacaaaacc tcacagttaa acgtcaacta 240
caagccttgc aacaaagtgg ttcttcagta ctttccgccg ataaaagcaa gcgactaaac 300
gagatattaa atactatgtc cacaatttac tccacaggaa aggtctgcaa ccctagcaat 360
ccgcaggaat gtctacttct ggagccgggg ctggacgcaa taatggaaaa ctccaaagac 420
tataaccaga ggctatgggc ctgggaagga tggcggtcag aggtaggcaa acaactccgc 480
ccgttgtacg aagagtacgt tgtgcttaaa aatgaaatgg cacgagcaaa caattatgaa 540
gattacgggg attattggcg tggagattac gaggcagagg gcccgagcgg ttacgattac 600
tcacgggatc agctgatcga agacgtagaa cgaacgttcg ctgaaatcaa gccactctac 660
gagcacttac atgcgtatgt tagagcgaag ttgatggaca catatccatc tcacatcaac 720
ccaaccggtt gccttccggc ccatttattg ggtgacatgt ggggcagatt ttggactaac 780
ttgtatagct taacggtacc cttcggtcag aaacccaata ttgatgtgac ggatgcaatg 840
gttgatcaaa gctgggacgc taaaaggatt ttcgaagaag ctgagaagtt cttcgtgtcg 900
gtcgggctcc caaatatgac tcaagggttt tgggagaata gcatgttgac ggagcctggc 960
gacggccgga aagtcgtttg ccaccctacc gcatgggacc tagggaaagg agatttccga 1020
attaagatgt gcactaaggt caccatggac gatttcctca cagctcatca tgagatgggc 1080
cacattcagt atgacatggc ctatgcagta cagccctacc tactgcgcaa cggtgcaaat 1140
gagggctttc acgaggccgt tggcgaaata atgtcattga gcgcggccac ccccaatcat 1200
ctaaaggcca ttggactttt acctcctgat ttctacgaag attctgaaac tgagattaac 1260
ttcctcttaa aacaggcttt aacgatagtg ggaacgctac catttacata tatgctggaa 1320
aagtggagat ggatggtctt taaaggtgaa attcctaaag aggagtggat gaagaaatgg 1380
tgggagatga agcgtgagat tgtgggggtg gttgagccag taccacatga cgaaacatac 1440
tgtgatccag cagccttgtt tcacgtcgcg aatgactact cgtttatacg ttattatacg 1500
cgcactatct atcaattcca atttcaggaa gcgctgtgcc agactgctaa acacgaagga 1560
ccgcttcaca agtgtgacat cagcaattcc accgaagctg gtcagaagtt gcttcaaatg 1620
ctctcgttag gaaaatccga accctggacc ttagcgctcg agcgcatcgt gggggtgaaa 1680
aacatggatg ttcggccgtt acttaactat tttgagcccc tgttcacctg gctgaaagat 1740
cagaataaaa acagtttcgt gggctggagt acaaattggt ctccctacgc tgat 1794
<210> 49
<211> 723
<212> PRT
<213> Artificial Sequence
<220>
<223> hACE2-740（人）氨基酸序列
<400> 49
Gln Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn
1 5 10 15
His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn
20 25 30
Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala
35 40 45
Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln
50 55 60
Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu
65 70 75 80
Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys Ser
85 90 95
Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr
100 105 110
Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu Glu
115 120 125
Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu Arg
130 135 140
Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu Arg
145 150 155 160
Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Ala
165 170 175
Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Val
180 185 190
Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu Asp
195 200 205
Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu His
210 215 220
Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile Ser
225 230 235 240
Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg
245 250 255
Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys Pro
260 265 270
Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala Gln
275 280 285
Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro
290 295 300
Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro Gly
305 310 315 320
Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys
325 330 335
Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp Phe
340 345 350
Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr
355 360 365
Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His
370 375 380
Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His
385 390 395 400
Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn Glu
405 410 415
Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr
420 425 430
Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys
435 440 445
Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met Lys
450 455 460
Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr
465 470 475 480
Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe Ile
485 490 495
Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala Leu
500 505 510
Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser
515 520 525
Asn Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu Gly
530 535 540
Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala Lys
545 550 555 560
Asn Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr
565 570 575
Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr Asp
580 585 590
Trp Ser Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu Lys
595 600 605
Ser Ala Leu Gly Asp Lys Ala Tyr Glu Trp Asn Asp Asn Glu Met Tyr
610 615 620
Leu Phe Arg Ser Ser Val Ala Tyr Ala Met Arg Gln Tyr Phe Leu Lys
625 630 635 640
Val Lys Asn Gln Met Ile Leu Phe Gly Glu Glu Asp Val Arg Val Ala
645 650 655
Asn Leu Lys Pro Arg Ile Ser Phe Asn Phe Phe Val Thr Ala Pro Lys
660 665 670
Asn Val Ser Asp Ile Ile Pro Arg Thr Glu Val Glu Lys Ala Ile Arg
675 680 685
Met Ser Arg Ser Arg Ile Asn Asp Ala Phe Arg Leu Asn Asp Asn Ser
690 695 700
Leu Glu Phe Leu Gly Ile Gln Pro Thr Leu Gly Pro Pro Asn Gln Pro
705 710 715 720
Pro Val Ser
<210> 50
<211> 598
<212> PRT
<213> Artificial Sequence
<220>
<223> hACE2-615（人）氨基酸序列
<400> 50
Gln Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn
1 5 10 15
His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn
20 25 30
Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala
35 40 45
Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln
50 55 60
Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu
65 70 75 80
Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys Ser
85 90 95
Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr
100 105 110
Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu Glu
115 120 125
Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu Arg
130 135 140
Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu Arg
145 150 155 160
Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Ala
165 170 175
Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Val
180 185 190
Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu Asp
195 200 205
Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu His
210 215 220
Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile Ser
225 230 235 240
Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg
245 250 255
Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys Pro
260 265 270
Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala Gln
275 280 285
Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro
290 295 300
Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro Gly
305 310 315 320
Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys
325 330 335
Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp Phe
340 345 350
Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr
355 360 365
Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His
370 375 380
Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His
385 390 395 400
Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn Glu
405 410 415
Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr
420 425 430
Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys
435 440 445
Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met Lys
450 455 460
Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr
465 470 475 480
Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe Ile
485 490 495
Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala Leu
500 505 510
Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser
515 520 525
Asn Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu Gly
530 535 540
Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala Lys
545 550 555 560
Asn Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr
565 570 575
Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr Asp
580 585 590
Trp Ser Pro Tyr Ala Asp
595
<210> 51
<211> 722
<212> PRT
<213> Artificial Sequence
<220>
<223> AtACE2-740（老虎）氨基酸序列
<400> 51
Ser Thr Thr Glu Glu Leu Ala Lys Thr Phe Leu Glu Lys Phe Asn His
1 5 10 15
Glu Ala Glu Glu Leu Ser Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr
20 25 30
Asn Thr Asn Ile Thr Asp Glu Asn Val Gln Lys Met Asn Glu Ala Gly
35 40 45
Ala Lys Trp Ser Ala Phe Tyr Glu Glu Gln Ser Lys Leu Ala Glu Thr
50 55 60
Tyr Pro Leu Ala Glu Ile His Asn Thr Thr Val Lys Arg Gln Leu Gln
65 70 75 80
Ala Leu Gln Gln Ser Gly Ser Ser Val Leu Ser Ala Asp Lys Ser Gln
85 90 95
Arg Leu Asn Thr Ile Leu Asn Ala Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Ala Cys Asn Pro Asn Asn Pro Gln Glu Cys Leu Leu Leu Glu Pro
115 120 125
Gly Leu Asp Asp Ile Met Glu Asn Ser Lys Asp Tyr Asn Glu Arg Leu
130 135 140
Trp Ala Trp Glu Gly Trp Arg Ala Glu Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Ala Leu Lys Asn Glu Met Ala Arg Ala Asn
165 170 175
Asn Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Glu Glu
180 185 190
Trp Thr Asp Gly Tyr Asn Tyr Ser Arg Ser Gln Leu Ile Lys Asp Val
195 200 205
Glu His Thr Phe Thr Gln Ile Lys Pro Leu Tyr Gln His Leu His Ala
210 215 220
Tyr Val Arg Ala Lys Leu Met Asp Ser Tyr Pro Ser Arg Ile Ser Pro
225 230 235 240
Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Pro Leu Thr Val Pro Phe Gly Gln Lys Pro Asn
260 265 270
Ile Asp Val Thr Asp Ala Met Val Asn Gln Ser Trp Asp Ala Arg Arg
275 280 285
Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn
290 295 300
Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Glu Pro Gly Asn
305 310 315 320
Ser Gln Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly
325 330 335
Asp Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala
355 360 365
Val Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Asn His Leu
385 390 395 400
Lys Thr Ile Gly Leu Leu Pro Pro Gly Phe Ser Glu Asp Ser Glu Thr
405 410 415
Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly
435 440 445
Glu Ile Pro Lys Glu Gln Trp Met Gln Lys Trp Trp Glu Met Lys Arg
450 455 460
Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ser Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Arg Ile Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser Asn
515 520 525
Ser Ser Glu Ala Gly Lys Lys Leu Leu Gln Met Leu Thr Leu Gly Lys
530 535 540
Ser Lys Pro Trp Thr Leu Ala Leu Glu His Val Val Gly Glu Lys Asn
545 550 555 560
Met Asn Val Thr Pro Leu Leu Lys Tyr Phe Glu Pro Leu Phe Thr Trp
565 570 575
Leu Lys Glu Gln Asn Arg Asn Ser Phe Val Gly Trp Asn Thr Asp Trp
580 585 590
Arg Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu Lys Ser
595 600 605
Ala Leu Gly Asp Lys Ala Tyr Glu Trp Asn Asp Asn Glu Met Tyr Leu
610 615 620
Phe Arg Ser Ser Val Ala Tyr Ala Met Arg Glu Tyr Phe Ser Lys Val
625 630 635 640
Lys Asn Gln Thr Ile Pro Phe Val Glu Asp Asn Val Trp Val Ser Asn
645 650 655
Leu Lys Pro Arg Ile Ser Phe Asn Phe Phe Val Thr Ala Ser Lys Asn
660 665 670
Val Ser Asp Val Ile Pro Arg Arg Glu Val Glu Glu Ala Ile Arg Met
675 680 685
Ser Arg Ser Arg Ile Asn Asp Ala Phe Arg Leu Asp Asp Asn Ser Leu
690 695 700
Glu Phe Leu Gly Ile Gln Pro Thr Leu Ser Pro Pro Tyr Gln Pro Pro
705 710 715 720
Val Thr
<210> 52
<211> 597
<212> PRT
<213> Artificial Sequence
<220>
<223> AtACE2-615（老虎）氨基酸序列
<400> 52
Ser Thr Thr Glu Glu Leu Ala Lys Thr Phe Leu Glu Lys Phe Asn His
1 5 10 15
Glu Ala Glu Glu Leu Ser Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr
20 25 30
Asn Thr Asn Ile Thr Asp Glu Asn Val Gln Lys Met Asn Glu Ala Gly
35 40 45
Ala Lys Trp Ser Ala Phe Tyr Glu Glu Gln Ser Lys Leu Ala Glu Thr
50 55 60
Tyr Pro Leu Ala Glu Ile His Asn Thr Thr Val Lys Arg Gln Leu Gln
65 70 75 80
Ala Leu Gln Gln Ser Gly Ser Ser Val Leu Ser Ala Asp Lys Ser Gln
85 90 95
Arg Leu Asn Thr Ile Leu Asn Ala Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Ala Cys Asn Pro Asn Asn Pro Gln Glu Cys Leu Leu Leu Glu Pro
115 120 125
Gly Leu Asp Asp Ile Met Glu Asn Ser Lys Asp Tyr Asn Glu Arg Leu
130 135 140
Trp Ala Trp Glu Gly Trp Arg Ala Glu Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Ala Leu Lys Asn Glu Met Ala Arg Ala Asn
165 170 175
Asn Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Glu Glu
180 185 190
Trp Thr Asp Gly Tyr Asn Tyr Ser Arg Ser Gln Leu Ile Lys Asp Val
195 200 205
Glu His Thr Phe Thr Gln Ile Lys Pro Leu Tyr Gln His Leu His Ala
210 215 220
Tyr Val Arg Ala Lys Leu Met Asp Ser Tyr Pro Ser Arg Ile Ser Pro
225 230 235 240
Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Pro Leu Thr Val Pro Phe Gly Gln Lys Pro Asn
260 265 270
Ile Asp Val Thr Asp Ala Met Val Asn Gln Ser Trp Asp Ala Arg Arg
275 280 285
Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn
290 295 300
Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Glu Pro Gly Asn
305 310 315 320
Ser Gln Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly
325 330 335
Asp Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala
355 360 365
Val Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Asn His Leu
385 390 395 400
Lys Thr Ile Gly Leu Leu Pro Pro Gly Phe Ser Glu Asp Ser Glu Thr
405 410 415
Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly
435 440 445
Glu Ile Pro Lys Glu Gln Trp Met Gln Lys Trp Trp Glu Met Lys Arg
450 455 460
Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ser Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Arg Ile Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser Asn
515 520 525
Ser Ser Glu Ala Gly Lys Lys Leu Leu Gln Met Leu Thr Leu Gly Lys
530 535 540
Ser Lys Pro Trp Thr Leu Ala Leu Glu His Val Val Gly Glu Lys Asn
545 550 555 560
Met Asn Val Thr Pro Leu Leu Lys Tyr Phe Glu Pro Leu Phe Thr Trp
565 570 575
Leu Lys Glu Gln Asn Arg Asn Ser Phe Val Gly Trp Asn Thr Asp Trp
580 585 590
Arg Pro Tyr Ala Asp
595
<210> 53
<211> 721
<212> PRT
<213> Artificial Sequence
<220>
<223> BtACE2-740（牛）氨基酸序列
<400> 53
Ser Thr Thr Glu Glu Gln Ala Lys Thr Phe Leu Glu Lys Phe Asn His
1 5 10 15
Glu Ala Glu Asp Leu Ser Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr
20 25 30
Asn Thr Asn Ile Thr Asp Glu Asn Val Gln Lys Met Asn Glu Ala Arg
35 40 45
Ala Lys Trp Ser Ala Phe Tyr Glu Glu Gln Ser Arg Met Ala Lys Thr
50 55 60
Tyr Ser Leu Glu Glu Ile Gln Asn Leu Thr Leu Lys Arg Gln Leu Lys
65 70 75 80
Ala Leu Gln His Ser Gly Thr Ser Ala Leu Ser Ala Glu Lys Ser Lys
85 90 95
Arg Leu Asn Thr Ile Leu Asn Lys Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Val Leu Asp Pro Asn Thr Gln Glu Cys Leu Ala Leu Glu Pro Gly
115 120 125
Leu Asp Asp Ile Met Glu Asn Ser Arg Asp Tyr Asn Arg Arg Leu Trp
130 135 140
Ala Trp Glu Gly Trp Arg Ala Glu Val Gly Lys Gln Leu Arg Pro Leu
145 150 155 160
Tyr Glu Glu Tyr Val Val Leu Glu Asn Glu Met Ala Arg Ala Asn Asn
165 170 175
Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Val Thr Gly
180 185 190
Ala Gly Asp Tyr Asp Tyr Ser Arg Asp Gln Leu Met Lys Asp Val Glu
195 200 205
Arg Thr Phe Ala Glu Ile Lys Pro Leu Tyr Glu Gln Leu His Ala Tyr
210 215 220
Val Arg Ala Lys Leu Met His Thr Tyr Pro Ser Tyr Ile Ser Pro Thr
225 230 235 240
Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe Trp
245 250 255
Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Glu His Lys Pro Ser Ile
260 265 270
Asp Val Thr Glu Lys Met Glu Asn Gln Ser Trp Asp Ala Glu Arg Ile
275 280 285
Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Ile Ser Leu Pro Tyr Met
290 295 300
Thr Gln Gly Phe Trp Asp Asn Ser Met Leu Thr Glu Pro Gly Asp Gly
305 310 315 320
Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly Asp
325 330 335
Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu Thr
340 345 350
Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala Ala
355 360 365
Gln Pro Tyr Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu Ala
370 375 380
Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro His Tyr Leu Lys
385 390 395 400
Ala Leu Gly Leu Leu Ala Pro Asp Phe His Glu Asp Asn Glu Thr Glu
405 410 415
Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu Pro
420 425 430
Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly Glu
435 440 445
Ile Pro Lys Gln Gln Trp Met Glu Lys Trp Trp Glu Met Lys Arg Glu
450 455 460
Ile Val Gly Val Val Glu Pro Leu Pro His Asp Glu Thr Tyr Cys Asp
465 470 475 480
Pro Ala Cys Leu Phe His Val Ala Glu Asp Tyr Ser Phe Ile Arg Tyr
485 490 495
Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe His Glu Ala Leu Cys Lys
500 505 510
Thr Ala Lys His Glu Gly Ala Leu Phe Lys Cys Asp Ile Ser Asn Ser
515 520 525
Thr Glu Ala Gly Gln Arg Leu Leu Gln Met Leu Arg Leu Gly Lys Ser
530 535 540
Glu Pro Trp Thr Leu Ala Leu Glu Asn Ile Val Gly Ile Lys Thr Met
545 550 555 560
Asp Val Lys Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr Trp Leu
565 570 575
Lys Glu Gln Asn Arg Asn Ser Phe Val Gly Trp Ser Thr Glu Trp Thr
580 585 590
Pro Tyr Ser Asp Gln Ser Ile Lys Val Arg Ile Ser Leu Lys Ser Ala
595 600 605
Leu Gly Glu Asn Ala Tyr Glu Trp Asn Asp Asn Glu Met Tyr Leu Phe
610 615 620
Gln Ser Ser Val Ala Tyr Ala Met Arg Lys Tyr Phe Ser Glu Ala Arg
625 630 635 640
Asn Glu Thr Val Leu Phe Gly Glu Asp Asn Val Trp Val Ser Asp Lys
645 650 655
Lys Pro Arg Ile Ser Phe Lys Phe Phe Val Thr Ser Pro Asn Asn Val
660 665 670
Ser Asp Ile Ile Pro Arg Thr Glu Val Glu Asn Ala Ile Arg Leu Ser
675 680 685
Arg Asp Arg Ile Asn Asp Val Phe Gln Leu Asp Asp Asn Ser Leu Glu
690 695 700
Phe Leu Gly Ile Gln Pro Thr Leu Gly Pro Pro Tyr Glu Pro Pro Val
705 710 715 720
Thr
<210> 54
<211> 596
<212> PRT
<213> Artificial Sequence
<220>
<223> BtACE2-615（牛）氨基酸序列
<400> 54
Ser Thr Thr Glu Glu Gln Ala Lys Thr Phe Leu Glu Lys Phe Asn His
1 5 10 15
Glu Ala Glu Asp Leu Ser Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr
20 25 30
Asn Thr Asn Ile Thr Asp Glu Asn Val Gln Lys Met Asn Glu Ala Arg
35 40 45
Ala Lys Trp Ser Ala Phe Tyr Glu Glu Gln Ser Arg Met Ala Lys Thr
50 55 60
Tyr Ser Leu Glu Glu Ile Gln Asn Leu Thr Leu Lys Arg Gln Leu Lys
65 70 75 80
Ala Leu Gln His Ser Gly Thr Ser Ala Leu Ser Ala Glu Lys Ser Lys
85 90 95
Arg Leu Asn Thr Ile Leu Asn Lys Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Val Leu Asp Pro Asn Thr Gln Glu Cys Leu Ala Leu Glu Pro Gly
115 120 125
Leu Asp Asp Ile Met Glu Asn Ser Arg Asp Tyr Asn Arg Arg Leu Trp
130 135 140
Ala Trp Glu Gly Trp Arg Ala Glu Val Gly Lys Gln Leu Arg Pro Leu
145 150 155 160
Tyr Glu Glu Tyr Val Val Leu Glu Asn Glu Met Ala Arg Ala Asn Asn
165 170 175
Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Val Thr Gly
180 185 190
Ala Gly Asp Tyr Asp Tyr Ser Arg Asp Gln Leu Met Lys Asp Val Glu
195 200 205
Arg Thr Phe Ala Glu Ile Lys Pro Leu Tyr Glu Gln Leu His Ala Tyr
210 215 220
Val Arg Ala Lys Leu Met His Thr Tyr Pro Ser Tyr Ile Ser Pro Thr
225 230 235 240
Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe Trp
245 250 255
Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Glu His Lys Pro Ser Ile
260 265 270
Asp Val Thr Glu Lys Met Glu Asn Gln Ser Trp Asp Ala Glu Arg Ile
275 280 285
Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Ile Ser Leu Pro Tyr Met
290 295 300
Thr Gln Gly Phe Trp Asp Asn Ser Met Leu Thr Glu Pro Gly Asp Gly
305 310 315 320
Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly Asp
325 330 335
Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu Thr
340 345 350
Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala Ala
355 360 365
Gln Pro Tyr Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu Ala
370 375 380
Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro His Tyr Leu Lys
385 390 395 400
Ala Leu Gly Leu Leu Ala Pro Asp Phe His Glu Asp Asn Glu Thr Glu
405 410 415
Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu Pro
420 425 430
Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly Glu
435 440 445
Ile Pro Lys Gln Gln Trp Met Glu Lys Trp Trp Glu Met Lys Arg Glu
450 455 460
Ile Val Gly Val Val Glu Pro Leu Pro His Asp Glu Thr Tyr Cys Asp
465 470 475 480
Pro Ala Cys Leu Phe His Val Ala Glu Asp Tyr Ser Phe Ile Arg Tyr
485 490 495
Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe His Glu Ala Leu Cys Lys
500 505 510
Thr Ala Lys His Glu Gly Ala Leu Phe Lys Cys Asp Ile Ser Asn Ser
515 520 525
Thr Glu Ala Gly Gln Arg Leu Leu Gln Met Leu Arg Leu Gly Lys Ser
530 535 540
Glu Pro Trp Thr Leu Ala Leu Glu Asn Ile Val Gly Ile Lys Thr Met
545 550 555 560
Asp Val Lys Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr Trp Leu
565 570 575
Lys Glu Gln Asn Arg Asn Ser Phe Val Gly Trp Ser Thr Glu Trp Thr
580 585 590
Pro Tyr Ser Asp
595
<210> 55
<211> 736
<212> PRT
<213> Artificial Sequence
<220>
<223> DrACE2-740（斑马鱼）氨基酸序列
<400> 55
Gln Thr Val Glu Asp Arg Ala Arg Glu Phe Leu Asn Lys Phe Asp Glu
1 5 10 15
Glu Ala Ser Asp Ile Met Tyr Gln Tyr Thr Leu Ala Ser Trp Ala Tyr
20 25 30
Asn Thr Asp Ile Ser Gln Glu Asn Ala Asp Lys Glu Ala Glu Ala Tyr
35 40 45
Ala Ile Trp Ser Glu Tyr Tyr Asn Lys Met Ser Glu Glu Ser Asn Ala
50 55 60
Tyr Pro Ile Asp Gln Ile Ser Asp Pro Ile Ile Lys Met Gln Leu Gln
65 70 75 80
Lys Leu Gln Asp Lys Gly Ser Gly Ala Leu Ser Pro Asp Lys Ala Ser
85 90 95
Glu Leu Arg Asn Ile Met Ser Glu Met Ser Thr Ile Tyr Asn Thr Ala
100 105 110
Thr Val Cys Lys Ile Asp Asp Pro Thr Asp Cys Gln Thr Leu Glu Pro
115 120 125
Gly Leu Glu Ser Ile Met Ala Glu Ser Arg Asp Tyr Asp Glu Arg Leu
130 135 140
His Val Trp Glu Gly Trp Arg Val Ala Thr Gly Met Lys Met Arg Pro
145 150 155 160
Leu Tyr Glu Lys Tyr Val Asp Leu Lys Asn Glu Ala Ala Lys Leu Asn
165 170 175
Asn Tyr Glu Asp His Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Thr Ile
180 185 190
Asp Asp Pro Lys Tyr Ser Tyr Ser Arg Asp Gln Val Ile Glu Asp Ala
195 200 205
Arg Arg Ile Tyr Lys Glu Ile Leu Pro Leu Tyr Lys Glu Leu His Ala
210 215 220
Tyr Val Arg Ala Lys Leu Gln Asp Val Tyr Pro Gly His Ile Gly Ser
225 230 235 240
Asp Ala Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Pro Leu Met Ile Pro Tyr Pro Asp Arg Pro Asp
260 265 270
Ile Asp Val Ser Ser Ala Met Val Glu Gln Gly Trp Asp Glu Ile Arg
275 280 285
Leu Phe Lys Glu Ala Glu Lys Phe Phe Met Ser Val Asn Met Pro Ala
290 295 300
Met Phe Asp Asn Phe Trp Asn Asn Ser Met Phe Ile Lys Pro Glu Glu
305 310 315 320
Arg Asp Val Val Cys His Pro Thr Ala Trp Asp Met Gly Asn Arg Lys
325 330 335
Asp Phe Arg Ile Lys Met Cys Thr Lys Val Asn Met Asp Asp Phe Leu
340 345 350
Thr Val His His Glu Met Gly His Asn Gln Tyr Gln Met Ala Tyr Arg
355 360 365
Asn His Pro Tyr Leu Leu Arg Asp Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Ser His Leu
385 390 395 400
Gln Ser Leu Gly Leu Leu Pro Ser Asp Phe Lys Gln Asp Tyr Glu Thr
405 410 415
Asp Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Glu Trp Arg Trp Gln Val Phe Lys Ala
435 440 445
Lys Ile Pro Lys Asp Glu Trp Met Gln Gln Trp Trp Gln Met Lys Arg
450 455 460
Glu Leu Val Gly Val Ala Glu Ala Val Pro Arg Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Pro Ala Leu Phe His Val Ser Gly Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Phe Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Lys Ala Ala Gly His Thr Gly Pro Leu Tyr Lys Cys Asp Ile Thr Asn
515 520 525
Ser Thr Lys Ala Gly Asp Lys Leu Arg His Met Leu Glu Leu Gly Arg
530 535 540
Ser Met Ser Trp Thr Arg Ala Leu Glu Glu Val Ala Gly Thr Thr Lys
545 550 555 560
Met Asp Ser Gln Pro Leu Leu His Tyr Phe Ser Thr Leu Met Glu Trp
565 570 575
Leu Lys Glu Glu Asn Gln Lys Asn Asn Arg Val Pro Gly Trp Asn Val
580 585 590
Asn Val Asn Pro Gly Val Leu Thr Ser Ser Phe Ile Asn Asp Ala Glu
595 600 605
Ile Ser Glu Asn Ala Phe Lys Val Arg Ile Ser Leu Lys Ser Ala Leu
610 615 620
Gly Asn Glu Ala Tyr Thr Trp Asn Ala Asn Asp Ile Tyr Leu Phe Lys
625 630 635 640
Ser Thr Met Ala Phe Ala Met Arg Gln Tyr Tyr Leu Lys Glu Lys Asn
645 650 655
Thr Asp Val Asn Phe Thr Pro Glu Asn Ile His Thr Tyr Asn Glu Thr
660 665 670
Ala Arg Ile Ser Phe Lys Phe Ala Val Met Asp Pro Thr Lys Thr Gly
675 680 685
Thr Val Ile Pro Lys Ala Glu Val Glu Asn Ala Ile Trp Gln Glu Arg
690 695 700
Asp Arg Ile Asn Gly Ala Phe Leu Leu Ser Asp Glu Thr Leu Glu Phe
705 710 715 720
Val Gly Leu Met Ala Thr Leu Ala Pro Pro Lys Glu Glu Lys Ile Thr
725 730 735
<210> 56
<211> 606
<212> PRT
<213> Artificial Sequence
<220>
<223> DrACE2-615（斑马鱼）氨基酸序列
<400> 56
Gln Thr Val Glu Asp Arg Ala Arg Glu Phe Leu Asn Lys Phe Asp Glu
1 5 10 15
Glu Ala Ser Asp Ile Met Tyr Gln Tyr Thr Leu Ala Ser Trp Ala Tyr
20 25 30
Asn Thr Asp Ile Ser Gln Glu Asn Ala Asp Lys Glu Ala Glu Ala Tyr
35 40 45
Ala Ile Trp Ser Glu Tyr Tyr Asn Lys Met Ser Glu Glu Ser Asn Ala
50 55 60
Tyr Pro Ile Asp Gln Ile Ser Asp Pro Ile Ile Lys Met Gln Leu Gln
65 70 75 80
Lys Leu Gln Asp Lys Gly Ser Gly Ala Leu Ser Pro Asp Lys Ala Ser
85 90 95
Glu Leu Arg Asn Ile Met Ser Glu Met Ser Thr Ile Tyr Asn Thr Ala
100 105 110
Thr Val Cys Lys Ile Asp Asp Pro Thr Asp Cys Gln Thr Leu Glu Pro
115 120 125
Gly Leu Glu Ser Ile Met Ala Glu Ser Arg Asp Tyr Asp Glu Arg Leu
130 135 140
His Val Trp Glu Gly Trp Arg Val Ala Thr Gly Met Lys Met Arg Pro
145 150 155 160
Leu Tyr Glu Lys Tyr Val Asp Leu Lys Asn Glu Ala Ala Lys Leu Asn
165 170 175
Asn Tyr Glu Asp His Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Thr Ile
180 185 190
Asp Asp Pro Lys Tyr Ser Tyr Ser Arg Asp Gln Val Ile Glu Asp Ala
195 200 205
Arg Arg Ile Tyr Lys Glu Ile Leu Pro Leu Tyr Lys Glu Leu His Ala
210 215 220
Tyr Val Arg Ala Lys Leu Gln Asp Val Tyr Pro Gly His Ile Gly Ser
225 230 235 240
Asp Ala Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Pro Leu Met Ile Pro Tyr Pro Asp Arg Pro Asp
260 265 270
Ile Asp Val Ser Ser Ala Met Val Glu Gln Gly Trp Asp Glu Ile Arg
275 280 285
Leu Phe Lys Glu Ala Glu Lys Phe Phe Met Ser Val Asn Met Pro Ala
290 295 300
Met Phe Asp Asn Phe Trp Asn Asn Ser Met Phe Ile Lys Pro Glu Glu
305 310 315 320
Arg Asp Val Val Cys His Pro Thr Ala Trp Asp Met Gly Asn Arg Lys
325 330 335
Asp Phe Arg Ile Lys Met Cys Thr Lys Val Asn Met Asp Asp Phe Leu
340 345 350
Thr Val His His Glu Met Gly His Asn Gln Tyr Gln Met Ala Tyr Arg
355 360 365
Asn His Pro Tyr Leu Leu Arg Asp Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Ser His Leu
385 390 395 400
Gln Ser Leu Gly Leu Leu Pro Ser Asp Phe Lys Gln Asp Tyr Glu Thr
405 410 415
Asp Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Glu Trp Arg Trp Gln Val Phe Lys Ala
435 440 445
Lys Ile Pro Lys Asp Glu Trp Met Gln Gln Trp Trp Gln Met Lys Arg
450 455 460
Glu Leu Val Gly Val Ala Glu Ala Val Pro Arg Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Pro Ala Leu Phe His Val Ser Gly Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Phe Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Lys Ala Ala Gly His Thr Gly Pro Leu Tyr Lys Cys Asp Ile Thr Asn
515 520 525
Ser Thr Lys Ala Gly Asp Lys Leu Arg His Met Leu Glu Leu Gly Arg
530 535 540
Ser Met Ser Trp Thr Arg Ala Leu Glu Glu Val Ala Gly Thr Thr Lys
545 550 555 560
Met Asp Ser Gln Pro Leu Leu His Tyr Phe Ser Thr Leu Met Glu Trp
565 570 575
Leu Lys Glu Glu Asn Gln Lys Asn Asn Arg Val Pro Gly Trp Asn Val
580 585 590
Asn Val Asn Pro Gly Val Leu Thr Ser Ser Phe Ile Asn Asp
595 600 605
<210> 57
<211> 721
<212> PRT
<213> Artificial Sequence
<220>
<223> dACE2-740（狗）氨基酸序列
<400> 57
Ser Thr Glu Asp Leu Val Lys Thr Phe Leu Glu Lys Phe Asn Tyr Glu
1 5 10 15
Ala Glu Glu Leu Ser Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr Asn
20 25 30
Ile Asn Ile Thr Asp Glu Asn Val Gln Lys Met Asn Asn Ala Gly Ala
35 40 45
Lys Trp Ser Ala Phe Tyr Glu Glu Gln Ser Lys Leu Ala Lys Thr Tyr
50 55 60
Pro Leu Glu Glu Ile Gln Asp Ser Thr Val Lys Arg Gln Leu Arg Ala
65 70 75 80
Leu Gln His Ser Gly Ser Ser Val Leu Ser Ala Asp Lys Asn Gln Arg
85 90 95
Leu Asn Thr Ile Leu Asn Ser Met Ser Thr Val Tyr Ser Thr Gly Lys
100 105 110
Ala Cys Asn Pro Ser Asn Pro Gln Glu Cys Leu Leu Leu Glu Pro Gly
115 120 125
Leu Asp Asp Ile Met Glu Asn Ser Lys Asp Tyr Asn Glu Arg Leu Trp
130 135 140
Ala Trp Glu Gly Trp Arg Ser Glu Val Gly Lys Gln Leu Arg Pro Leu
145 150 155 160
Tyr Glu Glu Tyr Val Ala Leu Lys Asn Glu Met Ala Arg Ala Asn Asn
165 170 175
Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Glu Glu Trp
180 185 190
Glu Asn Gly Tyr Asn Tyr Ser Arg Asn Gln Leu Ile Asp Asp Val Glu
195 200 205
Leu Thr Phe Thr Gln Ile Met Pro Leu Tyr Gln His Leu His Ala Tyr
210 215 220
Val Arg Thr Lys Leu Met Asp Thr Tyr Pro Ser Tyr Ile Ser Pro Thr
225 230 235 240
Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe Trp
245 250 255
Thr Asn Leu Tyr Pro Leu Thr Val Pro Phe Gly Gln Lys Pro Asn Ile
260 265 270
Asp Val Thr Asn Ala Met Val Asn Gln Ser Trp Asp Ala Arg Lys Ile
275 280 285
Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn Met
290 295 300
Thr Gln Glu Phe Trp Gly Asn Ser Met Leu Thr Glu Pro Ser Asp Ser
305 310 315 320
Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly Asp
325 330 335
Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu Thr
340 345 350
Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala Ala
355 360 365
Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu Ala
370 375 380
Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Asn His Leu Lys
385 390 395 400
Asn Ile Gly Leu Leu Pro Pro Ser Phe Phe Glu Asp Ser Glu Thr Glu
405 410 415
Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu Pro
420 425 430
Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly Glu
435 440 445
Ile Pro Lys Asp Gln Trp Met Lys Thr Trp Trp Glu Met Lys Arg Asn
450 455 460
Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys Asp
465 470 475 480
Pro Ala Ser Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile Arg Tyr
485 490 495
Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys Gln
500 505 510
Ile Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser Asn Ser
515 520 525
Ser Glu Ala Gly Gln Lys Leu Leu Glu Met Leu Lys Leu Gly Lys Ser
530 535 540
Lys Pro Trp Thr Tyr Ala Leu Glu Ile Val Val Gly Ala Lys Asn Met
545 550 555 560
Asp Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr Trp Leu
565 570 575
Lys Glu Gln Asn Arg Asn Ser Phe Val Gly Trp Asn Thr Asp Trp Ser
580 585 590
Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu Lys Ser Ala
595 600 605
Leu Gly Glu Lys Ala Tyr Glu Trp Asn Asn Asn Glu Met Tyr Leu Phe
610 615 620
Arg Ser Ser Ile Ala Tyr Ala Met Arg Gln Tyr Phe Ser Glu Val Lys
625 630 635 640
Asn Gln Thr Ile Pro Phe Val Glu Asp Asn Val Trp Val Ser Asp Leu
645 650 655
Lys Pro Arg Ile Ser Phe Asn Phe Ser Val Thr Ser Pro Gly Asn Val
660 665 670
Ser Asp Ile Ile Pro Arg Thr Glu Val Glu Glu Ala Ile Arg Met Tyr
675 680 685
Arg Ser Arg Ile Asn Asp Val Phe Arg Leu Asp Asp Asn Ser Leu Glu
690 695 700
Phe Leu Gly Ile Gln Pro Thr Pro Gly Pro Pro Tyr Glu Pro Pro Val
705 710 715 720
Thr
<210> 58
<211> 596
<212> PRT
<213> Artificial Sequence
<220>
<223> dACE2-615（狗）氨基酸序列
<400> 58
Ser Thr Glu Asp Leu Val Lys Thr Phe Leu Glu Lys Phe Asn Tyr Glu
1 5 10 15
Ala Glu Glu Leu Ser Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr Asn
20 25 30
Ile Asn Ile Thr Asp Glu Asn Val Gln Lys Met Asn Asn Ala Gly Ala
35 40 45
Lys Trp Ser Ala Phe Tyr Glu Glu Gln Ser Lys Leu Ala Lys Thr Tyr
50 55 60
Pro Leu Glu Glu Ile Gln Asp Ser Thr Val Lys Arg Gln Leu Arg Ala
65 70 75 80
Leu Gln His Ser Gly Ser Ser Val Leu Ser Ala Asp Lys Asn Gln Arg
85 90 95
Leu Asn Thr Ile Leu Asn Ser Met Ser Thr Val Tyr Ser Thr Gly Lys
100 105 110
Ala Cys Asn Pro Ser Asn Pro Gln Glu Cys Leu Leu Leu Glu Pro Gly
115 120 125
Leu Asp Asp Ile Met Glu Asn Ser Lys Asp Tyr Asn Glu Arg Leu Trp
130 135 140
Ala Trp Glu Gly Trp Arg Ser Glu Val Gly Lys Gln Leu Arg Pro Leu
145 150 155 160
Tyr Glu Glu Tyr Val Ala Leu Lys Asn Glu Met Ala Arg Ala Asn Asn
165 170 175
Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Glu Glu Trp
180 185 190
Glu Asn Gly Tyr Asn Tyr Ser Arg Asn Gln Leu Ile Asp Asp Val Glu
195 200 205
Leu Thr Phe Thr Gln Ile Met Pro Leu Tyr Gln His Leu His Ala Tyr
210 215 220
Val Arg Thr Lys Leu Met Asp Thr Tyr Pro Ser Tyr Ile Ser Pro Thr
225 230 235 240
Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe Trp
245 250 255
Thr Asn Leu Tyr Pro Leu Thr Val Pro Phe Gly Gln Lys Pro Asn Ile
260 265 270
Asp Val Thr Asn Ala Met Val Asn Gln Ser Trp Asp Ala Arg Lys Ile
275 280 285
Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn Met
290 295 300
Thr Gln Glu Phe Trp Gly Asn Ser Met Leu Thr Glu Pro Ser Asp Ser
305 310 315 320
Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly Asp
325 330 335
Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu Thr
340 345 350
Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala Ala
355 360 365
Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu Ala
370 375 380
Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Asn His Leu Lys
385 390 395 400
Asn Ile Gly Leu Leu Pro Pro Ser Phe Phe Glu Asp Ser Glu Thr Glu
405 410 415
Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu Pro
420 425 430
Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly Glu
435 440 445
Ile Pro Lys Asp Gln Trp Met Lys Thr Trp Trp Glu Met Lys Arg Asn
450 455 460
Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys Asp
465 470 475 480
Pro Ala Ser Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile Arg Tyr
485 490 495
Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys Gln
500 505 510
Ile Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser Asn Ser
515 520 525
Ser Glu Ala Gly Gln Lys Leu Leu Glu Met Leu Lys Leu Gly Lys Ser
530 535 540
Lys Pro Trp Thr Tyr Ala Leu Glu Ile Val Val Gly Ala Lys Asn Met
545 550 555 560
Asp Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr Trp Leu
565 570 575
Lys Glu Gln Asn Arg Asn Ser Phe Val Gly Trp Asn Thr Asp Trp Ser
580 585 590
Pro Tyr Ala Asp
595
<210> 59
<211> 722
<212> PRT
<213> Artificial Sequence
<220>
<223> DcACE2-740（猫）氨基酸序列
<400> 59
Ser Thr Thr Glu Glu Leu Ala Lys Thr Phe Leu Glu Lys Phe Asn His
1 5 10 15
Glu Ala Glu Glu Leu Ser Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr
20 25 30
Asn Thr Asn Ile Thr Asp Glu Asn Val Gln Lys Met Asn Glu Ala Gly
35 40 45
Ala Lys Trp Ser Ala Phe Tyr Glu Glu Gln Ser Lys Leu Ala Lys Thr
50 55 60
Tyr Pro Leu Ala Glu Ile His Asn Thr Thr Val Lys Arg Gln Leu Gln
65 70 75 80
Ala Leu Gln Gln Ser Gly Ser Ser Val Leu Ser Ala Asp Lys Ser Gln
85 90 95
Arg Leu Asn Thr Ile Leu Asn Ala Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Ala Cys Asn Pro Asn Asn Pro Gln Glu Cys Leu Leu Leu Glu Pro
115 120 125
Gly Leu Asp Asp Ile Met Glu Asn Ser Lys Asp Tyr Asn Glu Arg Leu
130 135 140
Trp Ala Trp Glu Gly Trp Arg Ala Glu Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Ala Leu Lys Asn Glu Met Ala Arg Ala Asn
165 170 175
Asn Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Glu Glu
180 185 190
Trp Thr Asp Gly Tyr Asn Tyr Ser Arg Ser Gln Leu Ile Lys Asp Val
195 200 205
Glu His Thr Phe Thr Gln Ile Lys Pro Leu Tyr Gln His Leu His Ala
210 215 220
Tyr Val Arg Ala Lys Leu Met Asp Thr Tyr Pro Ser Arg Ile Ser Pro
225 230 235 240
Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Pro Leu Thr Val Pro Phe Gly Gln Lys Pro Asn
260 265 270
Ile Asp Val Thr Asp Ala Met Val Asn Gln Ser Trp Asp Ala Arg Arg
275 280 285
Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn
290 295 300
Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Glu Pro Gly Asp
305 310 315 320
Ser Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly
325 330 335
Asp Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala
355 360 365
Val Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Asn His Leu
385 390 395 400
Lys Thr Ile Gly Leu Leu Ser Pro Gly Phe Ser Glu Asp Ser Glu Thr
405 410 415
Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly
435 440 445
Glu Ile Pro Lys Glu Gln Trp Met Gln Lys Trp Trp Glu Met Lys Arg
450 455 460
Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ser Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Arg Ile Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser Asn
515 520 525
Ser Ser Glu Ala Gly Lys Lys Leu Leu Gln Met Leu Thr Leu Gly Lys
530 535 540
Ser Lys Pro Trp Thr Leu Ala Leu Glu His Val Val Gly Glu Lys Lys
545 550 555 560
Met Asn Val Thr Pro Leu Leu Lys Tyr Phe Glu Pro Leu Phe Thr Trp
565 570 575
Leu Lys Glu Gln Asn Arg Asn Ser Phe Val Gly Trp Asn Thr Asp Trp
580 585 590
Arg Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu Lys Ser
595 600 605
Ala Leu Gly Asp Glu Ala Tyr Glu Trp Asn Asp Asn Glu Met Tyr Leu
610 615 620
Phe Arg Ser Ser Val Ala Tyr Ala Met Arg Glu Tyr Phe Ser Lys Val
625 630 635 640
Lys Asn Gln Thr Ile Pro Phe Val Glu Asp Asn Val Trp Val Ser Asn
645 650 655
Leu Lys Pro Arg Ile Ser Phe Asn Phe Phe Val Thr Ala Ser Lys Asn
660 665 670
Val Ser Asp Val Ile Pro Arg Ser Glu Val Glu Glu Ala Ile Arg Met
675 680 685
Ser Arg Ser Arg Ile Asn Asp Ala Phe Arg Leu Asp Asp Asn Ser Leu
690 695 700
Glu Phe Leu Gly Ile Gln Pro Thr Leu Ser Pro Pro Tyr Gln Pro Pro
705 710 715 720
Val Thr
<210> 60
<211> 597
<212> PRT
<213> Artificial Sequence
<220>
<223> DcACE2-615（猫）氨基酸序列
<400> 60
Ser Thr Thr Glu Glu Leu Ala Lys Thr Phe Leu Glu Lys Phe Asn His
1 5 10 15
Glu Ala Glu Glu Leu Ser Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr
20 25 30
Asn Thr Asn Ile Thr Asp Glu Asn Val Gln Lys Met Asn Glu Ala Gly
35 40 45
Ala Lys Trp Ser Ala Phe Tyr Glu Glu Gln Ser Lys Leu Ala Lys Thr
50 55 60
Tyr Pro Leu Ala Glu Ile His Asn Thr Thr Val Lys Arg Gln Leu Gln
65 70 75 80
Ala Leu Gln Gln Ser Gly Ser Ser Val Leu Ser Ala Asp Lys Ser Gln
85 90 95
Arg Leu Asn Thr Ile Leu Asn Ala Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Ala Cys Asn Pro Asn Asn Pro Gln Glu Cys Leu Leu Leu Glu Pro
115 120 125
Gly Leu Asp Asp Ile Met Glu Asn Ser Lys Asp Tyr Asn Glu Arg Leu
130 135 140
Trp Ala Trp Glu Gly Trp Arg Ala Glu Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Ala Leu Lys Asn Glu Met Ala Arg Ala Asn
165 170 175
Asn Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Glu Glu
180 185 190
Trp Thr Asp Gly Tyr Asn Tyr Ser Arg Ser Gln Leu Ile Lys Asp Val
195 200 205
Glu His Thr Phe Thr Gln Ile Lys Pro Leu Tyr Gln His Leu His Ala
210 215 220
Tyr Val Arg Ala Lys Leu Met Asp Thr Tyr Pro Ser Arg Ile Ser Pro
225 230 235 240
Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Pro Leu Thr Val Pro Phe Gly Gln Lys Pro Asn
260 265 270
Ile Asp Val Thr Asp Ala Met Val Asn Gln Ser Trp Asp Ala Arg Arg
275 280 285
Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn
290 295 300
Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Glu Pro Gly Asp
305 310 315 320
Ser Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly
325 330 335
Asp Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala
355 360 365
Val Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Asn His Leu
385 390 395 400
Lys Thr Ile Gly Leu Leu Ser Pro Gly Phe Ser Glu Asp Ser Glu Thr
405 410 415
Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly
435 440 445
Glu Ile Pro Lys Glu Gln Trp Met Gln Lys Trp Trp Glu Met Lys Arg
450 455 460
Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ser Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Arg Ile Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser Asn
515 520 525
Ser Ser Glu Ala Gly Lys Lys Leu Leu Gln Met Leu Thr Leu Gly Lys
530 535 540
Ser Lys Pro Trp Thr Leu Ala Leu Glu His Val Val Gly Glu Lys Lys
545 550 555 560
Met Asn Val Thr Pro Leu Leu Lys Tyr Phe Glu Pro Leu Phe Thr Trp
565 570 575
Leu Lys Glu Gln Asn Arg Asn Ser Phe Val Gly Trp Asn Thr Asp Trp
580 585 590
Arg Pro Tyr Ala Asp
595
<210> 61
<211> 722
<212> PRT
<213> Artificial Sequence
<220>
<223> DfACE2-740（雪貂）氨基酸序列
<400> 61
Ser Thr Thr Glu Asp Leu Ala Lys Thr Phe Leu Glu Lys Phe Asn Tyr
1 5 10 15
Glu Ala Glu Glu Leu Ser Tyr Gln Asn Ser Leu Ala Ser Trp Asn Tyr
20 25 30
Asn Thr Asn Ile Thr Asp Glu Asn Ile Gln Lys Met Asn Ile Ala Gly
35 40 45
Ala Lys Trp Ser Ala Phe Tyr Glu Glu Glu Ser Gln His Ala Lys Thr
50 55 60
Tyr Pro Leu Glu Glu Ile Gln Asp Pro Ile Ile Lys Arg Gln Leu Arg
65 70 75 80
Ala Leu Gln Gln Ser Gly Ser Ser Val Leu Ser Ala Asp Lys Arg Glu
85 90 95
Arg Leu Asn Thr Ile Leu Asn Ala Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Ala Cys Asn Pro Asn Asn Pro Gln Glu Cys Leu Leu Leu Glu Pro
115 120 125
Gly Leu Asp Asp Ile Met Glu Asn Ser Lys Asp Tyr Asn Glu Arg Leu
130 135 140
Trp Ala Trp Glu Gly Trp Arg Ser Glu Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Ala Leu Lys Asn Glu Met Ala Arg Ala Asn
165 170 175
Asn Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Glu Glu
180 185 190
Trp Ala Asp Gly Tyr Ser Tyr Ser Arg Asn Gln Leu Ile Glu Asp Val
195 200 205
Glu His Thr Phe Thr Gln Ile Lys Pro Leu Tyr Glu His Leu His Ala
210 215 220
Tyr Val Arg Ala Lys Leu Met Asp Ala Tyr Pro Ser Arg Ile Ser Pro
225 230 235 240
Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Pro Leu Met Val Pro Phe Arg Gln Lys Pro Asn
260 265 270
Ile Asp Val Thr Asp Ala Met Val Asn Gln Ser Trp Asp Ala Arg Arg
275 280 285
Ile Phe Glu Glu Ala Glu Thr Phe Phe Val Ser Val Gly Leu Pro Asn
290 295 300
Met Thr Glu Gly Phe Trp Gln Asn Ser Met Leu Thr Glu Pro Gly Asp
305 310 315 320
Asn Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Arg
325 330 335
Asp Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala
355 360 365
Glu Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Asn His Leu
385 390 395 400
Lys Asn Ile Gly Leu Leu Pro Pro Asp Phe Ser Glu Asp Ser Glu Thr
405 410 415
Asp Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly
435 440 445
Glu Ile Pro Lys Glu Gln Trp Met Gln Lys Trp Trp Glu Met Lys Arg
450 455 460
Asp Ile Val Gly Val Val Glu Pro Leu Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ala Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Gln Ile Ala Lys His Glu Gly Pro Leu Tyr Lys Cys Asp Ile Ser Asn
515 520 525
Ser Ser Glu Ala Gly Gln Lys Leu His Glu Met Leu Ser Leu Gly Arg
530 535 540
Ser Lys Pro Trp Thr Phe Ala Leu Glu Arg Val Val Gly Ala Lys Thr
545 550 555 560
Met Asp Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr Trp
565 570 575
Leu Lys Glu Gln Asn Arg Asn Ser Phe Val Gly Trp Asn Thr Asp Trp
580 585 590
Ser Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu Lys Ser
595 600 605
Ala Leu Gly Glu Lys Ala Tyr Glu Trp Asn Asp Asn Glu Met Tyr Phe
610 615 620
Phe Gln Ser Ser Ile Ala Tyr Ala Met Arg Glu Tyr Phe Ser Lys Val
625 630 635 640
Lys Asn Gln Thr Ile Pro Phe Val Gly Lys Asp Val Arg Val Ser Asp
645 650 655
Leu Lys Pro Arg Ile Ser Phe Asn Phe Ile Val Thr Ser Pro Glu Asn
660 665 670
Met Ser Asp Ile Ile Pro Arg Ala Asp Val Glu Glu Ala Ile Arg Lys
675 680 685
Ser Arg Gly Arg Ile Asn Asp Ala Phe Arg Leu Asp Asp Asn Ser Leu
690 695 700
Glu Phe Leu Gly Ile Gln Pro Thr Leu Glu Pro Pro Tyr Gln Pro Pro
705 710 715 720
Val Thr
<210> 62
<211> 597
<212> PRT
<213> Artificial Sequence
<220>
<223> DfACE2-615（雪貂）氨基酸序列
<400> 62
Ser Thr Thr Glu Asp Leu Ala Lys Thr Phe Leu Glu Lys Phe Asn Tyr
1 5 10 15
Glu Ala Glu Glu Leu Ser Tyr Gln Asn Ser Leu Ala Ser Trp Asn Tyr
20 25 30
Asn Thr Asn Ile Thr Asp Glu Asn Ile Gln Lys Met Asn Ile Ala Gly
35 40 45
Ala Lys Trp Ser Ala Phe Tyr Glu Glu Glu Ser Gln His Ala Lys Thr
50 55 60
Tyr Pro Leu Glu Glu Ile Gln Asp Pro Ile Ile Lys Arg Gln Leu Arg
65 70 75 80
Ala Leu Gln Gln Ser Gly Ser Ser Val Leu Ser Ala Asp Lys Arg Glu
85 90 95
Arg Leu Asn Thr Ile Leu Asn Ala Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Ala Cys Asn Pro Asn Asn Pro Gln Glu Cys Leu Leu Leu Glu Pro
115 120 125
Gly Leu Asp Asp Ile Met Glu Asn Ser Lys Asp Tyr Asn Glu Arg Leu
130 135 140
Trp Ala Trp Glu Gly Trp Arg Ser Glu Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Ala Leu Lys Asn Glu Met Ala Arg Ala Asn
165 170 175
Asn Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Glu Glu
180 185 190
Trp Ala Asp Gly Tyr Ser Tyr Ser Arg Asn Gln Leu Ile Glu Asp Val
195 200 205
Glu His Thr Phe Thr Gln Ile Lys Pro Leu Tyr Glu His Leu His Ala
210 215 220
Tyr Val Arg Ala Lys Leu Met Asp Ala Tyr Pro Ser Arg Ile Ser Pro
225 230 235 240
Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Pro Leu Met Val Pro Phe Arg Gln Lys Pro Asn
260 265 270
Ile Asp Val Thr Asp Ala Met Val Asn Gln Ser Trp Asp Ala Arg Arg
275 280 285
Ile Phe Glu Glu Ala Glu Thr Phe Phe Val Ser Val Gly Leu Pro Asn
290 295 300
Met Thr Glu Gly Phe Trp Gln Asn Ser Met Leu Thr Glu Pro Gly Asp
305 310 315 320
Asn Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Arg
325 330 335
Asp Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala
355 360 365
Glu Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Asn His Leu
385 390 395 400
Lys Asn Ile Gly Leu Leu Pro Pro Asp Phe Ser Glu Asp Ser Glu Thr
405 410 415
Asp Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly
435 440 445
Glu Ile Pro Lys Glu Gln Trp Met Gln Lys Trp Trp Glu Met Lys Arg
450 455 460
Asp Ile Val Gly Val Val Glu Pro Leu Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ala Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Gln Ile Ala Lys His Glu Gly Pro Leu Tyr Lys Cys Asp Ile Ser Asn
515 520 525
Ser Ser Glu Ala Gly Gln Lys Leu His Glu Met Leu Ser Leu Gly Arg
530 535 540
Ser Lys Pro Trp Thr Phe Ala Leu Glu Arg Val Val Gly Ala Lys Thr
545 550 555 560
Met Asp Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr Trp
565 570 575
Leu Lys Glu Gln Asn Arg Asn Ser Phe Val Gly Trp Asn Thr Asp Trp
580 585 590
Ser Pro Tyr Ala Asp
595
<210> 63
<211> 722
<212> PRT
<213> Artificial Sequence
<220>
<223> MmACE2-740（恒河猴）氨基酸序列
<400> 63
Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn His
1 5 10 15
Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr
20 25 30
Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala Gly
35 40 45
Glu Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln Met
50 55 60
Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu Gln
65 70 75 80
Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys Ser Lys
85 90 95
Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Val Cys Asn Pro Asn Asn Pro Gln Glu Cys Leu Leu Leu Asp Pro
115 120 125
Gly Leu Asn Glu Ile Met Glu Lys Ser Leu Asp Tyr Asn Glu Arg Leu
130 135 140
Trp Ala Trp Glu Gly Trp Arg Ser Glu Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Gly Ala Asn
165 170 175
His Tyr Lys Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Val Asn
180 185 190
Gly Val Asp Gly Tyr Asp Asn Asn Arg Asp Gln Leu Ile Glu Asp Val
195 200 205
Glu Arg Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu His Ala
210 215 220
Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile Ser Pro
225 230 235 240
Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys Pro Asn
260 265 270
Ile Asp Val Thr Asp Ala Met Val Asn Gln Ala Trp Asn Ala Gln Arg
275 280 285
Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn
290 295 300
Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro Gly Asn
305 310 315 320
Val Gln Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly
325 330 335
Asp Phe Arg Ile Ile Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala
355 360 365
Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu
385 390 395 400
Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn Glu Thr
405 410 415
Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly
435 440 445
Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met Lys Arg
450 455 460
Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser Asn
515 520 525
Ser Thr Glu Ala Gly Gln Lys Leu Leu Asn Met Leu Lys Leu Gly Glu
530 535 540
Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala Lys Asn
545 550 555 560
Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr Trp
565 570 575
Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr Asp Trp
580 585 590
Ser Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu Lys Ser
595 600 605
Ala Leu Gly Asp Lys Ala Tyr Glu Trp Asn Asp Asn Glu Met Tyr Leu
610 615 620
Phe Arg Ser Ser Val Ala Tyr Ala Met Arg Thr Tyr Phe Leu Glu Ile
625 630 635 640
Lys His Gln Thr Ile Leu Phe Gly Glu Glu Asp Val Arg Val Ala Asp
645 650 655
Leu Lys Pro Arg Ile Ser Phe Asn Phe Tyr Val Thr Ala Pro Lys Asn
660 665 670
Val Ser Asp Ile Ile Pro Arg Thr Glu Val Glu Glu Ala Ile Arg Ile
675 680 685
Ser Arg Ser Arg Ile Asn Asp Ala Phe Arg Leu Asn Asp Asn Ser Leu
690 695 700
Glu Phe Leu Gly Ile Gln Thr Thr Leu Ala Pro Pro Tyr Gln Ser Pro
705 710 715 720
Val Thr
<210> 64
<211> 597
<212> PRT
<213> Artificial Sequence
<220>
<223> MmACE2-615（恒河猴）氨基酸序列
<400> 64
Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn His
1 5 10 15
Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr
20 25 30
Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala Gly
35 40 45
Glu Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln Met
50 55 60
Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu Gln
65 70 75 80
Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys Ser Lys
85 90 95
Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Val Cys Asn Pro Asn Asn Pro Gln Glu Cys Leu Leu Leu Asp Pro
115 120 125
Gly Leu Asn Glu Ile Met Glu Lys Ser Leu Asp Tyr Asn Glu Arg Leu
130 135 140
Trp Ala Trp Glu Gly Trp Arg Ser Glu Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Gly Ala Asn
165 170 175
His Tyr Lys Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Val Asn
180 185 190
Gly Val Asp Gly Tyr Asp Asn Asn Arg Asp Gln Leu Ile Glu Asp Val
195 200 205
Glu Arg Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu His Ala
210 215 220
Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile Ser Pro
225 230 235 240
Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys Pro Asn
260 265 270
Ile Asp Val Thr Asp Ala Met Val Asn Gln Ala Trp Asn Ala Gln Arg
275 280 285
Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn
290 295 300
Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro Gly Asn
305 310 315 320
Val Gln Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly
325 330 335
Asp Phe Arg Ile Ile Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala
355 360 365
Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu
385 390 395 400
Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn Glu Thr
405 410 415
Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly
435 440 445
Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met Lys Arg
450 455 460
Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser Asn
515 520 525
Ser Thr Glu Ala Gly Gln Lys Leu Leu Asn Met Leu Lys Leu Gly Glu
530 535 540
Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala Lys Asn
545 550 555 560
Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr Trp
565 570 575
Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr Asp Trp
580 585 590
Ser Pro Tyr Ala Asp
595
<210> 65
<211> 722
<212> PRT
<213> Artificial Sequence
<220>
<223> MjACE2-740（穿山甲）氨基酸序列
<400> 65
Ser Thr Ser Asp Glu Glu Ala Lys Thr Phe Leu Glu Lys Phe Asn Ser
1 5 10 15
Glu Ala Glu Glu Leu Ser Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr
20 25 30
Asn Thr Asn Ile Thr Asp Glu Asn Val Gln Lys Met Asn Val Ala Gly
35 40 45
Ala Lys Trp Ser Thr Phe Tyr Glu Glu Gln Ser Lys Ile Ala Lys Asn
50 55 60
Tyr Gln Leu Gln Asn Ile Gln Asn Asp Thr Ile Lys Arg Gln Leu Gln
65 70 75 80
Ala Leu Gln Leu Ser Gly Ser Ser Ala Leu Ser Ala Asp Lys Asn Gln
85 90 95
Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Val Cys Asn Pro Gly Asn Pro Gln Glu Cys Ser Leu Leu Glu Pro
115 120 125
Gly Leu Asp Asn Ile Met Glu Ser Ser Lys Asp Tyr Asn Glu Arg Leu
130 135 140
Trp Ala Trp Glu Gly Trp Arg Ser Glu Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Ala Asn
165 170 175
His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Ala Glu
180 185 190
Gly Ala Asn Gly Tyr Asn Tyr Ser Arg Asp His Leu Ile Glu Asp Val
195 200 205
Glu His Ile Phe Thr Gln Ile Lys Pro Leu Tyr Glu His Leu His Ala
210 215 220
Tyr Val Arg Ala Lys Leu Met Asp Asn Tyr Pro Ser His Ile Ser Pro
225 230 235 240
Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Pro Leu Thr Val Pro Phe Arg Gln Lys Pro Asn
260 265 270
Ile Asp Val Thr Asp Ala Met Val Asn Gln Thr Trp Asp Ala Asn Arg
275 280 285
Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Lys
290 295 300
Met Thr Gln Thr Phe Trp Glu Asn Ser Met Leu Thr Glu Pro Gly Asp
305 310 315 320
Gly Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys His
325 330 335
Asp Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala
355 360 365
Met Gln Pro Tyr Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu
385 390 395 400
Lys Asn Ile Gly Leu Leu Pro Pro Asp Phe Tyr Glu Asp Asn Glu Thr
405 410 415
Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Ser Gly
435 440 445
Gln Ile Pro Lys Glu Gln Trp Met Lys Lys Trp Trp Glu Met Lys Arg
450 455 460
Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ser Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Gln Thr Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser Asn
515 520 525
Ser Ala Glu Ala Gly Gln Lys Leu Leu Gln Met Leu Ser Leu Gly Lys
530 535 540
Ser Lys Pro Trp Thr Leu Ala Leu Glu Arg Val Val Gly Thr Lys Asn
545 550 555 560
Met Asp Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Leu Thr Trp
565 570 575
Leu Lys Glu Gln Asn Lys Asn Ser Phe Val Gly Trp Asn Thr Asp Trp
580 585 590
Ser Pro Tyr Ala Ala Gln Ser Ile Lys Val Arg Ile Ser Leu Lys Ser
595 600 605
Ala Leu Gly Glu Lys Ala Tyr Glu Trp Asn Asp Ser Glu Met Tyr Leu
610 615 620
Phe Arg Ser Ser Val Ala Tyr Ala Met Arg Glu Tyr Phe Ser Lys Val
625 630 635 640
Lys Lys Gln Thr Ile Pro Phe Glu Asp Glu Cys Val Arg Val Ser Asp
645 650 655
Leu Lys Pro Arg Val Ser Phe Ile Phe Phe Val Thr Leu Pro Lys Asn
660 665 670
Val Ser Ala Val Ile Pro Arg Ala Glu Val Glu Glu Ala Ile Arg Ile
675 680 685
Ser Arg Ser Arg Ile Asn Asp Ala Phe Arg Leu Asp Asp Asn Ser Leu
690 695 700
Glu Phe Leu Gly Ile Gln Pro Thr Leu Gln Pro Pro Tyr Gln Pro Pro
705 710 715 720
Val Thr
<210> 66
<211> 597
<212> PRT
<213> Artificial Sequence
<220>
<223> MjACE2-615（穿山甲）氨基酸序列
<400> 66
Ser Thr Ser Asp Glu Glu Ala Lys Thr Phe Leu Glu Lys Phe Asn Ser
1 5 10 15
Glu Ala Glu Glu Leu Ser Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr
20 25 30
Asn Thr Asn Ile Thr Asp Glu Asn Val Gln Lys Met Asn Val Ala Gly
35 40 45
Ala Lys Trp Ser Thr Phe Tyr Glu Glu Gln Ser Lys Ile Ala Lys Asn
50 55 60
Tyr Gln Leu Gln Asn Ile Gln Asn Asp Thr Ile Lys Arg Gln Leu Gln
65 70 75 80
Ala Leu Gln Leu Ser Gly Ser Ser Ala Leu Ser Ala Asp Lys Asn Gln
85 90 95
Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Val Cys Asn Pro Gly Asn Pro Gln Glu Cys Ser Leu Leu Glu Pro
115 120 125
Gly Leu Asp Asn Ile Met Glu Ser Ser Lys Asp Tyr Asn Glu Arg Leu
130 135 140
Trp Ala Trp Glu Gly Trp Arg Ser Glu Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Ala Asn
165 170 175
His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Ala Glu
180 185 190
Gly Ala Asn Gly Tyr Asn Tyr Ser Arg Asp His Leu Ile Glu Asp Val
195 200 205
Glu His Ile Phe Thr Gln Ile Lys Pro Leu Tyr Glu His Leu His Ala
210 215 220
Tyr Val Arg Ala Lys Leu Met Asp Asn Tyr Pro Ser His Ile Ser Pro
225 230 235 240
Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Pro Leu Thr Val Pro Phe Arg Gln Lys Pro Asn
260 265 270
Ile Asp Val Thr Asp Ala Met Val Asn Gln Thr Trp Asp Ala Asn Arg
275 280 285
Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Lys
290 295 300
Met Thr Gln Thr Phe Trp Glu Asn Ser Met Leu Thr Glu Pro Gly Asp
305 310 315 320
Gly Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys His
325 330 335
Asp Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala
355 360 365
Met Gln Pro Tyr Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu
385 390 395 400
Lys Asn Ile Gly Leu Leu Pro Pro Asp Phe Tyr Glu Asp Asn Glu Thr
405 410 415
Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Ser Gly
435 440 445
Gln Ile Pro Lys Glu Gln Trp Met Lys Lys Trp Trp Glu Met Lys Arg
450 455 460
Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ser Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Gln Thr Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser Asn
515 520 525
Ser Ala Glu Ala Gly Gln Lys Leu Leu Gln Met Leu Ser Leu Gly Lys
530 535 540
Ser Lys Pro Trp Thr Leu Ala Leu Glu Arg Val Val Gly Thr Lys Asn
545 550 555 560
Met Asp Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Leu Thr Trp
565 570 575
Leu Lys Glu Gln Asn Lys Asn Ser Phe Val Gly Trp Asn Thr Asp Trp
580 585 590
Ser Pro Tyr Ala Ala
595
<210> 67
<211> 722
<212> PRT
<213> Artificial Sequence
<220>
<223> MfACE2-740（土拨鼠）氨基酸序列
<400> 67
Ser Thr Ile Glu Glu Leu Ala Lys Thr Phe Leu Asp Lys Phe Asn Gln
1 5 10 15
Glu Ala Glu Asp Leu Asp Tyr Gln Arg Ser Leu Ala Ser Trp Asn Tyr
20 25 30
Asn Thr Asn Ile Thr Lys Glu Asn Thr Gln Lys Met Asn Glu Ala Glu
35 40 45
Ala Lys Trp Ser Ala Phe Tyr Glu Lys Gln Ser Lys Leu Ala Lys Ala
50 55 60
Tyr Pro Leu Gln Glu Ile Gln Asn Phe Thr Leu Lys Arg Gln Leu Gln
65 70 75 80
Ala Leu Gln Gln Ser Gly Ser Ser Ala Leu Ser Ala Asn Lys Arg Glu
85 90 95
Gln Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Val Cys Asn Pro Lys Lys Pro Gln Glu Cys Leu Leu Leu Glu Pro
115 120 125
Gly Leu Asp Gly Ile Met Ala Asn Ser Thr Asp Tyr Asn Glu Arg Leu
130 135 140
Trp Val Trp Glu Gly Trp Arg Ser Lys Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Ala Asn
165 170 175
Asn Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Ala Glu
180 185 190
Gly Ala Asp Gly Tyr Gly Tyr Asn His Asn Gln Leu Ile Glu Asp Val
195 200 205
Glu Arg Thr Phe Ala Glu Ile Lys Pro Leu Tyr Glu His Leu His Ala
210 215 220
Tyr Val Arg Ala Lys Leu Met Asn Thr Tyr Pro Ser Tyr Ile Ser Pro
225 230 235 240
Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Pro Glu Lys Pro Asn
260 265 270
Ile Asp Val Thr Asp Ala Met Ile Lys Gln Asn Trp Asn Ala Val Arg
275 280 285
Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn
290 295 300
Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Glu Pro Thr Asp
305 310 315 320
Gly Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gln Lys Gly
325 330 335
Asp Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asn Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asn Met Ala Tyr Ala
355 360 365
Ile Gln Pro Tyr Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Thr Thr Pro Lys His Leu
385 390 395 400
Lys Ser Ile Gly Leu Leu Pro Ser Asp Phe Arg Glu Asp Asn Glu Thr
405 410 415
Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Ala Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly
435 440 445
Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met Lys Arg
450 455 460
Glu Ile Val Gly Val Met Glu Pro Val Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ala Leu Tyr His Val Ser Asn Asp Phe Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser Asn
515 520 525
Ser Thr Glu Ala Gly Gln Lys Leu Leu Asn Met Leu Arg Leu Gly Lys
530 535 540
Ser Lys Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala Arg Asn
545 550 555 560
Met Asp Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Gly Trp
565 570 575
Leu Lys Asp Gln Asn Arg Asn Ser Phe Val Gly Trp Asn Thr Asn Trp
580 585 590
Ser Pro Tyr Thr Asp Gln Ser Ile Lys Val Arg Ile Ser Leu Lys Ser
595 600 605
Ala Leu Gly Glu Glu Ala Tyr Gln Trp Asn Asp Asn Glu Met Tyr Leu
610 615 620
Phe Arg Ser Ser Val Ala Tyr Ala Met Arg Met Tyr Phe Ser Lys Val
625 630 635 640
Lys Asn Gln Thr Ile Pro Phe Gly Glu Glu Asp Val Trp Val Ser Asp
645 650 655
Leu Lys Pro Arg Ile Ser Phe Asn Phe Phe Val Thr Thr Pro Gln Asn
660 665 670
Ala Ser Asp Ile Ile Pro Arg Thr Asp Val Glu Lys Ala Ile Arg Met
675 680 685
Ser Arg Gly Arg Ile Asn Gly Val Phe Arg Leu Asp Asp Asn Ser Leu
690 695 700
Glu Phe Leu Gly Ile Gln Pro Thr Leu Gly Pro Pro Tyr Gln Pro Pro
705 710 715 720
Val Thr
<210> 68
<211> 597
<212> PRT
<213> Artificial Sequence
<220>
<223> MfACE2-615（土拨鼠）氨基酸序列
<400> 68
Ser Thr Ile Glu Glu Leu Ala Lys Thr Phe Leu Asp Lys Phe Asn Gln
1 5 10 15
Glu Ala Glu Asp Leu Asp Tyr Gln Arg Ser Leu Ala Ser Trp Asn Tyr
20 25 30
Asn Thr Asn Ile Thr Lys Glu Asn Thr Gln Lys Met Asn Glu Ala Glu
35 40 45
Ala Lys Trp Ser Ala Phe Tyr Glu Lys Gln Ser Lys Leu Ala Lys Ala
50 55 60
Tyr Pro Leu Gln Glu Ile Gln Asn Phe Thr Leu Lys Arg Gln Leu Gln
65 70 75 80
Ala Leu Gln Gln Ser Gly Ser Ser Ala Leu Ser Ala Asn Lys Arg Glu
85 90 95
Gln Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Val Cys Asn Pro Lys Lys Pro Gln Glu Cys Leu Leu Leu Glu Pro
115 120 125
Gly Leu Asp Gly Ile Met Ala Asn Ser Thr Asp Tyr Asn Glu Arg Leu
130 135 140
Trp Val Trp Glu Gly Trp Arg Ser Lys Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Ala Asn
165 170 175
Asn Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Ala Glu
180 185 190
Gly Ala Asp Gly Tyr Gly Tyr Asn His Asn Gln Leu Ile Glu Asp Val
195 200 205
Glu Arg Thr Phe Ala Glu Ile Lys Pro Leu Tyr Glu His Leu His Ala
210 215 220
Tyr Val Arg Ala Lys Leu Met Asn Thr Tyr Pro Ser Tyr Ile Ser Pro
225 230 235 240
Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Pro Glu Lys Pro Asn
260 265 270
Ile Asp Val Thr Asp Ala Met Ile Lys Gln Asn Trp Asn Ala Val Arg
275 280 285
Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn
290 295 300
Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Glu Pro Thr Asp
305 310 315 320
Gly Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gln Lys Gly
325 330 335
Asp Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asn Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asn Met Ala Tyr Ala
355 360 365
Ile Gln Pro Tyr Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Thr Thr Pro Lys His Leu
385 390 395 400
Lys Ser Ile Gly Leu Leu Pro Ser Asp Phe Arg Glu Asp Asn Glu Thr
405 410 415
Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Ala Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly
435 440 445
Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met Lys Arg
450 455 460
Glu Ile Val Gly Val Met Glu Pro Val Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ala Leu Tyr His Val Ser Asn Asp Phe Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser Asn
515 520 525
Ser Thr Glu Ala Gly Gln Lys Leu Leu Asn Met Leu Arg Leu Gly Lys
530 535 540
Ser Lys Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala Arg Asn
545 550 555 560
Met Asp Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Gly Trp
565 570 575
Leu Lys Asp Gln Asn Arg Asn Ser Phe Val Gly Trp Asn Thr Asn Trp
580 585 590
Ser Pro Tyr Thr Asp
595
<210> 69
<211> 722
<212> PRT
<213> Artificial Sequence
<220>
<223> PlACE2-740（果子狸）氨基酸序列
<400> 69
Ser Thr Thr Glu Glu Leu Ala Lys Thr Phe Leu Glu Thr Phe Asn Tyr
1 5 10 15
Glu Ala Gln Glu Leu Ser Tyr Gln Ser Ser Val Ala Ser Trp Asn Tyr
20 25 30
Asn Thr Asn Ile Thr Asp Glu Asn Ala Lys Asn Met Asn Glu Ala Gly
35 40 45
Ala Lys Trp Ser Ala Tyr Tyr Glu Glu Gln Ser Lys Leu Ala Gln Thr
50 55 60
Tyr Pro Leu Ala Glu Ile Gln Asp Ala Lys Ile Lys Arg Gln Leu Gln
65 70 75 80
Ala Leu Gln Gln Ser Gly Ser Ser Val Leu Ser Ala Asp Lys Ser Gln
85 90 95
Arg Leu Asn Thr Ile Leu Asn Ala Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Ala Cys Asn Pro Asn Asn Pro Gln Glu Cys Leu Leu Leu Glu Pro
115 120 125
Gly Leu Asp Asn Ile Met Glu Asn Ser Lys Asp Tyr Asn Glu Arg Leu
130 135 140
Trp Ala Trp Glu Gly Trp Arg Ala Glu Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Ala Leu Lys Asn Glu Met Ala Arg Ala Asn
165 170 175
Asn Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Glu Glu
180 185 190
Trp Thr Gly Gly Tyr Asn Tyr Ser Arg Asn Gln Leu Ile Gln Asp Val
195 200 205
Glu Asp Thr Phe Glu Gln Ile Lys Pro Leu Tyr Gln His Leu His Ala
210 215 220
Tyr Val Arg Ala Lys Leu Met Asp Thr Tyr Pro Ser Arg Ile Ser Arg
225 230 235 240
Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Pro Leu Thr Val Pro Phe Gly Gln Lys Pro Asn
260 265 270
Ile Asp Val Thr Asp Ala Met Val Asn Gln Asn Trp Asp Ala Arg Arg
275 280 285
Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn
290 295 300
Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Glu Pro Gly Asp
305 310 315 320
Gly Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly
325 330 335
Asp Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala
355 360 365
Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Asn His Leu
385 390 395 400
Lys Thr Ile Gly Leu Leu Ser Pro Ala Phe Ser Glu Asp Asn Glu Thr
405 410 415
Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly
435 440 445
Ala Ile Pro Lys Glu Gln Trp Met Gln Lys Trp Trp Glu Met Lys Arg
450 455 460
Asn Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ser Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Gln Ile Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser Asn
515 520 525
Ser Thr Glu Ala Gly Lys Lys Leu Leu Glu Met Leu Ser Leu Gly Arg
530 535 540
Ser Glu Pro Trp Thr Leu Ala Leu Glu Arg Val Val Gly Ala Lys Asn
545 550 555 560
Met Asn Val Thr Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr Trp
565 570 575
Leu Lys Glu Gln Asn Arg Asn Ser Phe Val Gly Trp Asp Thr Asp Trp
580 585 590
Arg Pro Tyr Ser Asp Gln Ser Ile Lys Val Arg Ile Ser Leu Lys Ser
595 600 605
Ala Leu Gly Glu Lys Ala Tyr Glu Trp Asn Asp Asn Glu Met Tyr Leu
610 615 620
Phe Arg Ser Ser Ile Ala Tyr Ala Met Arg Glu Tyr Phe Ser Lys Val
625 630 635 640
Lys Asn Gln Thr Ile Pro Phe Val Glu Asp Asn Val Trp Val Ser Asp
645 650 655
Leu Lys Pro Arg Ile Ser Phe Asn Phe Phe Val Thr Phe Ser Asn Asn
660 665 670
Val Ser Asp Val Ile Pro Arg Ser Glu Val Glu Asp Ala Ile Arg Met
675 680 685
Ser Arg Ser Arg Ile Asn Asp Ala Phe Arg Leu Asp Asp Asn Ser Leu
690 695 700
Glu Phe Leu Gly Ile Glu Pro Thr Leu Ser Pro Pro Tyr Arg Pro Pro
705 710 715 720
Val Thr
<210> 70
<211> 597
<212> PRT
<213> Artificial Sequence
<220>
<223> PlACE2-615（果子狸）氨基酸序列
<400> 70
Ser Thr Thr Glu Glu Leu Ala Lys Thr Phe Leu Glu Thr Phe Asn Tyr
1 5 10 15
Glu Ala Gln Glu Leu Ser Tyr Gln Ser Ser Val Ala Ser Trp Asn Tyr
20 25 30
Asn Thr Asn Ile Thr Asp Glu Asn Ala Lys Asn Met Asn Glu Ala Gly
35 40 45
Ala Lys Trp Ser Ala Tyr Tyr Glu Glu Gln Ser Lys Leu Ala Gln Thr
50 55 60
Tyr Pro Leu Ala Glu Ile Gln Asp Ala Lys Ile Lys Arg Gln Leu Gln
65 70 75 80
Ala Leu Gln Gln Ser Gly Ser Ser Val Leu Ser Ala Asp Lys Ser Gln
85 90 95
Arg Leu Asn Thr Ile Leu Asn Ala Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Ala Cys Asn Pro Asn Asn Pro Gln Glu Cys Leu Leu Leu Glu Pro
115 120 125
Gly Leu Asp Asn Ile Met Glu Asn Ser Lys Asp Tyr Asn Glu Arg Leu
130 135 140
Trp Ala Trp Glu Gly Trp Arg Ala Glu Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Ala Leu Lys Asn Glu Met Ala Arg Ala Asn
165 170 175
Asn Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Glu Glu
180 185 190
Trp Thr Gly Gly Tyr Asn Tyr Ser Arg Asn Gln Leu Ile Gln Asp Val
195 200 205
Glu Asp Thr Phe Glu Gln Ile Lys Pro Leu Tyr Gln His Leu His Ala
210 215 220
Tyr Val Arg Ala Lys Leu Met Asp Thr Tyr Pro Ser Arg Ile Ser Arg
225 230 235 240
Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Pro Leu Thr Val Pro Phe Gly Gln Lys Pro Asn
260 265 270
Ile Asp Val Thr Asp Ala Met Val Asn Gln Asn Trp Asp Ala Arg Arg
275 280 285
Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn
290 295 300
Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Glu Pro Gly Asp
305 310 315 320
Gly Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly
325 330 335
Asp Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala
355 360 365
Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Asn His Leu
385 390 395 400
Lys Thr Ile Gly Leu Leu Ser Pro Ala Phe Ser Glu Asp Asn Glu Thr
405 410 415
Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly
435 440 445
Ala Ile Pro Lys Glu Gln Trp Met Gln Lys Trp Trp Glu Met Lys Arg
450 455 460
Asn Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ser Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Gln Ile Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser Asn
515 520 525
Ser Thr Glu Ala Gly Lys Lys Leu Leu Glu Met Leu Ser Leu Gly Arg
530 535 540
Ser Glu Pro Trp Thr Leu Ala Leu Glu Arg Val Val Gly Ala Lys Asn
545 550 555 560
Met Asn Val Thr Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr Trp
565 570 575
Leu Lys Glu Gln Asn Arg Asn Ser Phe Val Gly Trp Asp Thr Asp Trp
580 585 590
Arg Pro Tyr Ser Asp
595
<210> 71
<211> 722
<212> PRT
<213> Artificial Sequence
<220>
<223> PsACE2-740（中华鳖）氨基酸序列
<400> 71
Asp Ile Thr Gln Glu Ala Ile Asn Phe Leu Ser Glu Phe Asn Val Gln
1 5 10 15
Ala Glu Asp Leu Ser Tyr Ala Ser Ser Leu Ala Ser Trp Asn Tyr Asn
20 25 30
Thr Asn Ile Thr Asp Glu Asn Ala Lys Lys Met Asn Glu Ala Gly Ala
35 40 45
Lys Trp Ser Val Phe Tyr Asp Glu Ala Ser Thr Asn Ala Ser Lys Tyr
50 55 60
Ala Ile Asp Lys Ile Thr Asn His Thr Val Lys Leu Gln Leu Gln Ser
65 70 75 80
Leu Gln Gly Lys Gly Thr Ser Val Leu Ser Gly Glu Lys Tyr Asn Glu
85 90 95
Leu Asn Lys Ile Leu Ser Thr Met Ser Thr Phe Tyr Ser Thr Gly Thr
100 105 110
Val Cys Lys Pro Asp Asn Pro Asp Ile Cys Leu Pro Leu Glu Pro Gly
115 120 125
Leu Asp Ala Ile Met Ala Ser Ser Thr Asp Tyr Phe Glu Arg Leu Trp
130 135 140
Ala Trp Glu Gly Trp Arg Ala Asp Val Gly Lys Lys Met Arg Glu Leu
145 150 155 160
Tyr Glu Arg Tyr Val Glu Leu Glu Asn Glu Ala Ala Arg Leu Asn Lys
165 170 175
Tyr Ser Asp Tyr Gly Asp Tyr Trp Arg Gly Asn Tyr Glu Val Asn Asp
180 185 190
Pro Thr Glu Tyr Ala Tyr Ser Arg Asn Gln Leu Met Glu Asp Val Glu
195 200 205
Ala Thr Phe Glu Gln Ile Lys Pro Leu Tyr Arg Glu Leu His Ala Tyr
210 215 220
Val Arg Tyr Arg Leu Glu Lys Phe Tyr Gly Ser Asp His Ile Ser Ser
225 230 235 240
Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Ala Leu Thr Val Pro Tyr Pro Asp Lys Pro Asn
260 265 270
Ile Asp Val Thr Ser Glu Met Val Lys Lys Asn Trp Asn Ala Thr Lys
275 280 285
Ile Phe Lys Ala Ala Glu Asp Phe Phe Met Ser Val Gly Leu Tyr Lys
290 295 300
Met Thr Glu Gly Phe Trp Lys Asn Ser Met Ile Thr Glu Pro Asn Asp
305 310 315 320
Gly Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Met Gly Lys Lys
325 330 335
Asp Tyr Arg Ile Lys Met Cys Thr Lys Val Ser Met Asp Asp Phe Leu
340 345 350
Thr Val His His Glu Met Gly His Ile Glu Tyr Asp Met Ala Tyr Ser
355 360 365
Asn Leu Ser Tyr Leu Leu Arg Ser Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu
385 390 395 400
Lys Ser Leu Asp Leu Leu Glu Pro Thr Phe Gln Glu Asp Asn Glu Thr
405 410 415
Asp Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Met
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly
435 440 445
Asp Ile Pro Lys Asp Glu Trp Met Lys Lys Trp Trp Glu Met Lys Arg
450 455 460
Ala Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ala Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Lys Ala Ala Asn His Gly Gly Leu Leu His Thr Cys Asp Ile Thr Asn
515 520 525
Ser Met Ala Ala Gly Gln Lys Leu Arg Asp Met Leu Ala Leu Gly Arg
530 535 540
Ser Gln Pro Trp Thr Lys Ala Leu Glu Ser Ile Thr Gly Glu Lys Lys
545 550 555 560
Met Asn Ala Thr Pro Leu Leu His Tyr Phe Glu Pro Leu Tyr Gln Trp
565 570 575
Leu Ile Lys Asn Asn Ser Gly Arg Ala Val Gly Trp Asn Thr Phe Trp
580 585 590
Ser Pro Tyr Ser Gly Asn Ala Ile Lys Val Arg Ile Ser Leu Lys Thr
595 600 605
Ala Leu Gly Asp Asn Ala Tyr Glu Trp Asp Glu Asn Glu Leu Tyr Phe
610 615 620
Phe Lys Ser Ser Ile Ala Tyr Ala Met Arg Lys Tyr Phe Leu Glu Val
625 630 635 640
Lys Asn Gln Thr Val Ser Phe Gln Cys Thr Asp Ile His Val Trp Ala
645 650 655
Val Thr Gln Arg Val Ser Phe Tyr Phe Ala Val Ser Met Pro Gly Asn
660 665 670
Ala Thr Asp Phe Ile Pro Lys Ser Glu Val Glu Thr Ala Ile Arg Met
675 680 685
Ser Arg Gly Arg Ile Asn Glu Ala Phe Arg Leu Asp Asp Asn Thr Leu
690 695 700
Glu Phe Glu Gly Leu Leu Pro Thr Leu Ala Ser Pro Tyr Glu Pro Pro
705 710 715 720
Val Thr
<210> 72
<211> 597
<212> PRT
<213> Artificial Sequence
<220>
<223> PsACE2-615（中华鳖）氨基酸序列
<400> 72
Asp Ile Thr Gln Glu Ala Ile Asn Phe Leu Ser Glu Phe Asn Val Gln
1 5 10 15
Ala Glu Asp Leu Ser Tyr Ala Ser Ser Leu Ala Ser Trp Asn Tyr Asn
20 25 30
Thr Asn Ile Thr Asp Glu Asn Ala Lys Lys Met Asn Glu Ala Gly Ala
35 40 45
Lys Trp Ser Val Phe Tyr Asp Glu Ala Ser Thr Asn Ala Ser Lys Tyr
50 55 60
Ala Ile Asp Lys Ile Thr Asn His Thr Val Lys Leu Gln Leu Gln Ser
65 70 75 80
Leu Gln Gly Lys Gly Thr Ser Val Leu Ser Gly Glu Lys Tyr Asn Glu
85 90 95
Leu Asn Lys Ile Leu Ser Thr Met Ser Thr Phe Tyr Ser Thr Gly Thr
100 105 110
Val Cys Lys Pro Asp Asn Pro Asp Ile Cys Leu Pro Leu Glu Pro Gly
115 120 125
Leu Asp Ala Ile Met Ala Ser Ser Thr Asp Tyr Phe Glu Arg Leu Trp
130 135 140
Ala Trp Glu Gly Trp Arg Ala Asp Val Gly Lys Lys Met Arg Glu Leu
145 150 155 160
Tyr Glu Arg Tyr Val Glu Leu Glu Asn Glu Ala Ala Arg Leu Asn Lys
165 170 175
Tyr Ser Asp Tyr Gly Asp Tyr Trp Arg Gly Asn Tyr Glu Val Asn Asp
180 185 190
Pro Thr Glu Tyr Ala Tyr Ser Arg Asn Gln Leu Met Glu Asp Val Glu
195 200 205
Ala Thr Phe Glu Gln Ile Lys Pro Leu Tyr Arg Glu Leu His Ala Tyr
210 215 220
Val Arg Tyr Arg Leu Glu Lys Phe Tyr Gly Ser Asp His Ile Ser Ser
225 230 235 240
Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Ala Leu Thr Val Pro Tyr Pro Asp Lys Pro Asn
260 265 270
Ile Asp Val Thr Ser Glu Met Val Lys Lys Asn Trp Asn Ala Thr Lys
275 280 285
Ile Phe Lys Ala Ala Glu Asp Phe Phe Met Ser Val Gly Leu Tyr Lys
290 295 300
Met Thr Glu Gly Phe Trp Lys Asn Ser Met Ile Thr Glu Pro Asn Asp
305 310 315 320
Gly Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Met Gly Lys Lys
325 330 335
Asp Tyr Arg Ile Lys Met Cys Thr Lys Val Ser Met Asp Asp Phe Leu
340 345 350
Thr Val His His Glu Met Gly His Ile Glu Tyr Asp Met Ala Tyr Ser
355 360 365
Asn Leu Ser Tyr Leu Leu Arg Ser Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu
385 390 395 400
Lys Ser Leu Asp Leu Leu Glu Pro Thr Phe Gln Glu Asp Asn Glu Thr
405 410 415
Asp Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Met
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly
435 440 445
Asp Ile Pro Lys Asp Glu Trp Met Lys Lys Trp Trp Glu Met Lys Arg
450 455 460
Ala Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ala Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Lys Ala Ala Asn His Gly Gly Leu Leu His Thr Cys Asp Ile Thr Asn
515 520 525
Ser Met Ala Ala Gly Gln Lys Leu Arg Asp Met Leu Ala Leu Gly Arg
530 535 540
Ser Gln Pro Trp Thr Lys Ala Leu Glu Ser Ile Thr Gly Glu Lys Lys
545 550 555 560
Met Asn Ala Thr Pro Leu Leu His Tyr Phe Glu Pro Leu Tyr Gln Trp
565 570 575
Leu Ile Lys Asn Asn Ser Gly Arg Ala Val Gly Trp Asn Thr Phe Trp
580 585 590
Ser Pro Tyr Ser Gly
595
<210> 73
<211> 722
<212> PRT
<213> Artificial Sequence
<220>
<223> RnACE2-740（褐家鼠）氨基酸序列
<400> 73
Ser Leu Ile Glu Glu Lys Ala Glu Ser Phe Leu Asn Lys Phe Asn Gln
1 5 10 15
Glu Ala Glu Asp Leu Ser Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr
20 25 30
Asn Thr Asn Ile Thr Glu Glu Asn Ala Gln Lys Met Asn Glu Ala Ala
35 40 45
Ala Lys Trp Ser Ala Phe Tyr Glu Glu Gln Ser Lys Ile Ala Gln Asn
50 55 60
Phe Ser Leu Gln Glu Ile Gln Asn Ala Thr Ile Lys Arg Gln Leu Lys
65 70 75 80
Ala Leu Gln Gln Ser Gly Ser Ser Ala Leu Ser Pro Asp Lys Asn Lys
85 90 95
Gln Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Val Cys Asn Ser Met Asn Pro Gln Glu Cys Phe Leu Leu Glu Pro
115 120 125
Gly Leu Asp Glu Ile Met Ala Thr Ser Thr Asp Tyr Asn Arg Arg Leu
130 135 140
Trp Ala Trp Glu Gly Trp Arg Ala Glu Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Ala Asn
165 170 175
Asn Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Ala Glu
180 185 190
Gly Val Glu Gly Tyr Asn Tyr Asn Arg Asn Gln Leu Ile Glu Asp Val
195 200 205
Glu Asn Thr Phe Lys Glu Ile Lys Pro Leu Tyr Glu Gln Leu His Ala
210 215 220
Tyr Val Arg Thr Lys Leu Met Glu Val Tyr Pro Ser Tyr Ile Ser Pro
225 230 235 240
Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Pro Leu Thr Thr Pro Phe Leu Gln Lys Pro Asn
260 265 270
Ile Asp Val Thr Asp Ala Met Val Asn Gln Ser Trp Asp Ala Glu Arg
275 280 285
Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Gln
290 295 300
Met Thr Pro Gly Phe Trp Thr Asn Ser Met Leu Thr Glu Pro Gly Asp
305 310 315 320
Asp Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly His Gly
325 330 335
Asp Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asn Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala
355 360 365
Lys Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu
385 390 395 400
Lys Ser Ile Gly Leu Leu Pro Ser Asn Phe Gln Glu Asp Asn Glu Thr
405 410 415
Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Gln Asp
435 440 445
Lys Ile Pro Arg Glu Gln Trp Thr Lys Lys Trp Trp Glu Met Lys Arg
450 455 460
Glu Ile Val Gly Val Val Glu Pro Leu Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Gln Ala Ala Lys His Asp Gly Pro Leu His Lys Cys Asp Ile Ser Asn
515 520 525
Ser Thr Glu Ala Gly Gln Lys Leu Leu Asn Met Leu Ser Leu Gly Asn
530 535 540
Ser Gly Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ser Arg Asn
545 550 555 560
Met Asp Val Lys Pro Leu Leu Asn Tyr Phe Gln Pro Leu Phe Val Trp
565 570 575
Leu Lys Glu Gln Asn Arg Asn Ser Thr Val Gly Trp Ser Thr Asp Trp
580 585 590
Ser Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu Lys Ser
595 600 605
Ala Leu Gly Lys Asn Ala Tyr Glu Trp Thr Asp Asn Glu Met Tyr Leu
610 615 620
Phe Arg Ser Ser Val Ala Tyr Ala Met Arg Glu Tyr Phe Ser Arg Glu
625 630 635 640
Lys Asn Gln Thr Val Pro Phe Gly Glu Ala Asp Val Trp Val Ser Asp
645 650 655
Leu Lys Pro Arg Val Ser Phe Asn Phe Phe Val Thr Ser Pro Lys Asn
660 665 670
Val Ser Asp Ile Ile Pro Arg Ser Glu Val Glu Glu Ala Ile Arg Met
675 680 685
Ser Arg Gly Arg Ile Asn Asp Ile Phe Gly Leu Asn Asp Asn Ser Leu
690 695 700
Glu Phe Leu Gly Ile Tyr Pro Thr Leu Lys Pro Pro Tyr Glu Pro Pro
705 710 715 720
Val Thr
<210> 74
<211> 597
<212> PRT
<213> Artificial Sequence
<220>
<223> RnACE2-615（褐家鼠）氨基酸序列
<400> 74
Ser Leu Ile Glu Glu Lys Ala Glu Ser Phe Leu Asn Lys Phe Asn Gln
1 5 10 15
Glu Ala Glu Asp Leu Ser Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr
20 25 30
Asn Thr Asn Ile Thr Glu Glu Asn Ala Gln Lys Met Asn Glu Ala Ala
35 40 45
Ala Lys Trp Ser Ala Phe Tyr Glu Glu Gln Ser Lys Ile Ala Gln Asn
50 55 60
Phe Ser Leu Gln Glu Ile Gln Asn Ala Thr Ile Lys Arg Gln Leu Lys
65 70 75 80
Ala Leu Gln Gln Ser Gly Ser Ser Ala Leu Ser Pro Asp Lys Asn Lys
85 90 95
Gln Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Val Cys Asn Ser Met Asn Pro Gln Glu Cys Phe Leu Leu Glu Pro
115 120 125
Gly Leu Asp Glu Ile Met Ala Thr Ser Thr Asp Tyr Asn Arg Arg Leu
130 135 140
Trp Ala Trp Glu Gly Trp Arg Ala Glu Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Ala Asn
165 170 175
Asn Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Ala Glu
180 185 190
Gly Val Glu Gly Tyr Asn Tyr Asn Arg Asn Gln Leu Ile Glu Asp Val
195 200 205
Glu Asn Thr Phe Lys Glu Ile Lys Pro Leu Tyr Glu Gln Leu His Ala
210 215 220
Tyr Val Arg Thr Lys Leu Met Glu Val Tyr Pro Ser Tyr Ile Ser Pro
225 230 235 240
Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Pro Leu Thr Thr Pro Phe Leu Gln Lys Pro Asn
260 265 270
Ile Asp Val Thr Asp Ala Met Val Asn Gln Ser Trp Asp Ala Glu Arg
275 280 285
Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Gln
290 295 300
Met Thr Pro Gly Phe Trp Thr Asn Ser Met Leu Thr Glu Pro Gly Asp
305 310 315 320
Asp Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly His Gly
325 330 335
Asp Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asn Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala
355 360 365
Lys Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu
385 390 395 400
Lys Ser Ile Gly Leu Leu Pro Ser Asn Phe Gln Glu Asp Asn Glu Thr
405 410 415
Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Gln Asp
435 440 445
Lys Ile Pro Arg Glu Gln Trp Thr Lys Lys Trp Trp Glu Met Lys Arg
450 455 460
Glu Ile Val Gly Val Val Glu Pro Leu Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Gln Ala Ala Lys His Asp Gly Pro Leu His Lys Cys Asp Ile Ser Asn
515 520 525
Ser Thr Glu Ala Gly Gln Lys Leu Leu Asn Met Leu Ser Leu Gly Asn
530 535 540
Ser Gly Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ser Arg Asn
545 550 555 560
Met Asp Val Lys Pro Leu Leu Asn Tyr Phe Gln Pro Leu Phe Val Trp
565 570 575
Leu Lys Glu Gln Asn Arg Asn Ser Thr Val Gly Trp Ser Thr Asp Trp
580 585 590
Ser Pro Tyr Ala Asp
595
<210> 75
<211> 722
<212> PRT
<213> Artificial Sequence
<220>
<223> RfACE2-740（马铁菊头蝠）氨基酸序列
<400> 75
Ser Thr Thr Glu Asp Leu Ala Lys Lys Phe Leu Asp Asp Phe Asn Ser
1 5 10 15
Glu Ala Glu Asn Leu Ser His Gln Ser Ser Leu Ala Ser Trp Glu Tyr
20 25 30
Asn Thr Asn Ile Ser Asp Glu Asn Val Gln Lys Met Asp Glu Ala Gly
35 40 45
Ala Lys Trp Ser Asp Phe Tyr Glu Lys Gln Ser Lys Leu Ala Lys Asn
50 55 60
Phe Ser Leu Glu Glu Ile His Asn Asp Thr Val Lys Leu Gln Leu Gln
65 70 75 80
Ile Leu Gln Gln Ser Gly Ser Pro Val Leu Ser Glu Asp Lys Ser Lys
85 90 95
Arg Leu Asn Ser Ile Leu Asn Ala Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Val Cys Lys Pro Asn Asn Pro Gln Glu Cys Leu Leu Leu Glu Pro
115 120 125
Gly Leu Asp Asn Ile Met Gly Thr Ser Lys Asp Tyr Asn Glu Arg Leu
130 135 140
Trp Ala Trp Glu Gly Trp Arg Ala Glu Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Gly Tyr
165 170 175
His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Arg Asp Tyr Glu Thr Glu
180 185 190
Gly Ser Pro Asp Leu Glu Tyr Ser Arg Asp Gln Leu Ile Lys Asp Val
195 200 205
Glu Arg Ile Phe Ala Glu Ile Lys Pro Leu Tyr Glu Gln Leu His Ala
210 215 220
Tyr Val Arg Thr Lys Leu Met Asp Thr Tyr Pro Phe His Ile Ser Pro
225 230 235 240
Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Pro Leu Thr Val Pro Phe Gly Gln Lys Pro Asn
260 265 270
Ile Asp Val Thr Asp Ala Met Leu Asn Gln Asn Trp Asp Ala Lys Arg
275 280 285
Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Ile Gly Leu Pro Asn
290 295 300
Met Thr Glu Gly Phe Trp Asn Asn Ser Met Leu Thr Asp Pro Gly Asp
305 310 315 320
Gly Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly
325 330 335
Asp Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Glu Asp Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala
355 360 365
Ser Gln Pro Tyr Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Val Met Ser Leu Ser Val Ala Thr Pro Lys His Leu
385 390 395 400
Lys Thr Met Gly Leu Leu Ser Ser Asp Phe Leu Glu Asp Asn Glu Thr
405 410 415
Glu Ile Asn Phe Leu Phe Lys Gln Ala Leu Asn Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly
435 440 445
Glu Ile Pro Lys Glu Glu Trp Met Lys Lys Trp Trp Glu Met Lys Arg
450 455 460
Lys Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ser Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Ile Phe Glu Phe Gln Phe His Glu Ala Leu Cys
500 505 510
Arg Ile Ala Lys His Asp Gly Pro Leu His Lys Cys Asp Ile Ser Asn
515 520 525
Ser Thr Asp Ala Gly Glu Lys Leu His Gln Met Leu Ser Val Gly Lys
530 535 540
Ser Gln Pro Trp Thr Ser Val Leu Lys Asp Phe Val Gly Ser Lys Asn
545 550 555 560
Met Asp Val Gly Pro Leu Leu Arg Tyr Phe Glu Pro Leu Tyr Thr Trp
565 570 575
Leu Thr Glu Gln Asn Arg Lys Ser Phe Val Gly Trp Asn Thr Asp Trp
580 585 590
Ser Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu Lys Ser
595 600 605
Ala Leu Gly Glu Lys Ala Tyr Glu Trp Asn Asn Asn Glu Met Tyr Leu
610 615 620
Phe Arg Ser Ser Val Ala Tyr Ala Met Arg Glu Tyr Phe Leu Lys Thr
625 630 635 640
Lys Asn Gln Thr Ile Leu Phe Gly Glu Glu Asp Val Trp Val Ser Asn
645 650 655
Leu Lys Pro Arg Ile Ser Phe Asn Phe Tyr Val Thr Ser Pro Arg Asn
660 665 670
Leu Ser Asp Ile Ile Pro Lys Pro Glu Val Glu Gly Ala Ile Arg Met
675 680 685
Ser Arg Ser Arg Ile Asn Asp Ala Phe Arg Leu Asp Asp Asn Ser Leu
690 695 700
Glu Phe Leu Gly Ile Gln Pro Thr Leu Gly Pro Pro Tyr Gln Pro Pro
705 710 715 720
Val Thr
<210> 76
<211> 597
<212> PRT
<213> Artificial Sequence
<220>
<223> RfACE2-615（马铁菊头蝠）氨基酸序列
<400> 76
Ser Thr Thr Glu Asp Leu Ala Lys Lys Phe Leu Asp Asp Phe Asn Ser
1 5 10 15
Glu Ala Glu Asn Leu Ser His Gln Ser Ser Leu Ala Ser Trp Glu Tyr
20 25 30
Asn Thr Asn Ile Ser Asp Glu Asn Val Gln Lys Met Asp Glu Ala Gly
35 40 45
Ala Lys Trp Ser Asp Phe Tyr Glu Lys Gln Ser Lys Leu Ala Lys Asn
50 55 60
Phe Ser Leu Glu Glu Ile His Asn Asp Thr Val Lys Leu Gln Leu Gln
65 70 75 80
Ile Leu Gln Gln Ser Gly Ser Pro Val Leu Ser Glu Asp Lys Ser Lys
85 90 95
Arg Leu Asn Ser Ile Leu Asn Ala Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Val Cys Lys Pro Asn Asn Pro Gln Glu Cys Leu Leu Leu Glu Pro
115 120 125
Gly Leu Asp Asn Ile Met Gly Thr Ser Lys Asp Tyr Asn Glu Arg Leu
130 135 140
Trp Ala Trp Glu Gly Trp Arg Ala Glu Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Gly Tyr
165 170 175
His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Arg Asp Tyr Glu Thr Glu
180 185 190
Gly Ser Pro Asp Leu Glu Tyr Ser Arg Asp Gln Leu Ile Lys Asp Val
195 200 205
Glu Arg Ile Phe Ala Glu Ile Lys Pro Leu Tyr Glu Gln Leu His Ala
210 215 220
Tyr Val Arg Thr Lys Leu Met Asp Thr Tyr Pro Phe His Ile Ser Pro
225 230 235 240
Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Pro Leu Thr Val Pro Phe Gly Gln Lys Pro Asn
260 265 270
Ile Asp Val Thr Asp Ala Met Leu Asn Gln Asn Trp Asp Ala Lys Arg
275 280 285
Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Ile Gly Leu Pro Asn
290 295 300
Met Thr Glu Gly Phe Trp Asn Asn Ser Met Leu Thr Asp Pro Gly Asp
305 310 315 320
Gly Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly
325 330 335
Asp Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Glu Asp Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala
355 360 365
Ser Gln Pro Tyr Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Val Met Ser Leu Ser Val Ala Thr Pro Lys His Leu
385 390 395 400
Lys Thr Met Gly Leu Leu Ser Ser Asp Phe Leu Glu Asp Asn Glu Thr
405 410 415
Glu Ile Asn Phe Leu Phe Lys Gln Ala Leu Asn Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly
435 440 445
Glu Ile Pro Lys Glu Glu Trp Met Lys Lys Trp Trp Glu Met Lys Arg
450 455 460
Lys Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ser Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Ile Phe Glu Phe Gln Phe His Glu Ala Leu Cys
500 505 510
Arg Ile Ala Lys His Asp Gly Pro Leu His Lys Cys Asp Ile Ser Asn
515 520 525
Ser Thr Asp Ala Gly Glu Lys Leu His Gln Met Leu Ser Val Gly Lys
530 535 540
Ser Gln Pro Trp Thr Ser Val Leu Lys Asp Phe Val Gly Ser Lys Asn
545 550 555 560
Met Asp Val Gly Pro Leu Leu Arg Tyr Phe Glu Pro Leu Tyr Thr Trp
565 570 575
Leu Thr Glu Gln Asn Arg Lys Ser Phe Val Gly Trp Asn Thr Asp Trp
580 585 590
Ser Pro Tyr Ala Asp
595
<210> 77
<211> 722
<212> PRT
<213> Artificial Sequence
<220>
<223> sACE2-740（蝾螈）氨基酸序列
<400> 77
Asp Val Thr Asn Asp Ala Arg Val Phe Leu Asp Ala Phe Asn Ala Gln
1 5 10 15
Ala Glu Asp Leu Ser Tyr Glu Asn Ser Leu Ala Ser Trp Ala Tyr Asn
20 25 30
Thr Asn Ile Thr Glu Glu Asn Ala Ile Lys Met Asn Glu Ala Gly Ala
35 40 45
Lys Trp Thr Ala Phe Tyr Lys Lys Ala Asn Asn Asn Ala Ser Arg Phe
50 55 60
Pro Val Asp Gln Ile Thr Asp Pro Asp Ile Lys Leu Gln Ile Leu Ser
65 70 75 80
Leu Gly Glu Lys Gly Ser Ser Val Leu Pro Asp Asp Lys Tyr Asn Arg
85 90 95
Leu Asn Lys Ala Leu Ser Asp Met Ser Thr Ile Tyr Ser Thr Gly Thr
100 105 110
Val Cys Asp Asn Ser Ala Lys Cys Leu Gln Leu Glu Pro Gly Leu Asp
115 120 125
Leu Ile Met Ala Asp Ser Thr Asp Tyr His Lys Arg Leu Trp Ala Trp
130 135 140
Glu Gly Trp Arg Ser Glu Val Gly Lys Lys Met Arg Pro Leu Tyr Glu
145 150 155 160
Thr Tyr Val Asp Leu Asn Asn Glu Ala Ala Lys Leu Asn Asp Tyr Ala
165 170 175
Asp Tyr Gly Asp Tyr Trp Arg Gly Asn Tyr Glu Thr Gln Asp Ser Gly
180 185 190
Lys Tyr Ala Tyr Ser Arg Asn Asp Leu Lys Arg Asp Val Glu Arg Thr
195 200 205
Phe Lys Glu Ile Gln Pro Leu Tyr Arg Glu Leu His Ala Tyr Val Arg
210 215 220
Asp Lys Leu Arg Gly Val Tyr Gly Asp Lys Tyr Ile Ser Lys Asn Gly
225 230 235 240
Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe Trp Thr
245 250 255
Asn Leu Tyr Pro Leu Ala Val Pro Tyr Pro Asn Gln Pro Ser Ile Asp
260 265 270
Val Thr Ser Ala Met Asn Ala Lys Lys Trp Asn Val Asp Lys Met Phe
275 280 285
Arg Glu Ala Glu Asp Phe Phe Val Ser Val Gly Leu Tyr Lys Met Asn
290 295 300
Glu Asn Phe Trp Asn Phe Ser Met Leu Thr Glu Pro Asn Asp Gly Arg
305 310 315 320
Asn Val Val Cys His Pro Thr Ala Trp Asp Met Gly Lys Asn Asp Phe
325 330 335
Arg Ile Lys Met Cys Thr Lys Val Asn Met Glu Asp Phe Leu Thr Val
340 345 350
His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala Asn Leu
355 360 365
Ser Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu Ala Val
370 375 380
Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu Lys Ser
385 390 395 400
Leu Asp Leu Leu Pro Pro Thr Phe Val Glu Asn Glu Glu Thr Asn Ile
405 410 415
Asn Phe Leu Leu Arg Gln Ala Leu Thr Ile Val Ala Thr Met Pro Phe
420 425 430
Thr Tyr Met Leu Glu Glu Trp Arg Trp Lys Val Phe Asn Gly Glu Ile
435 440 445
Pro Arg Asp Gln Trp Met Lys Lys Trp Trp Gln Met Lys Arg Glu Ile
450 455 460
Val Gly Val Met Glu Pro Val Pro His Asp Glu Thr Tyr Cys Asp Pro
465 470 475 480
Ala Ala Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile Arg Tyr Tyr
485 490 495
Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys Lys Ala
500 505 510
Ala Asn His Asn Gly Ser Leu His Thr Cys Asp Ile Thr Asn Ser Thr
515 520 525
Leu Ala Gly Gln Lys Leu Arg Thr Met Leu Ala Leu Gly Asn Ser Lys
530 535 540
Pro Trp Thr Met Ala Leu Glu Ser Ile Thr Gly Gly Lys Thr Met Asp
545 550 555 560
Ala Gln Pro Leu Leu His Tyr Phe Asp Pro Leu Tyr Thr Trp Leu Arg
565 570 575
Lys Asn Asn Ile Asp Asn Asn Arg Gln Thr Tyr Trp Asp Thr Glu Trp
580 585 590
Ser Ala Tyr Thr Asp Tyr Glu Ile Lys Val Arg Ile Ser Leu His Ser
595 600 605
Ala Phe Gly Asp Asn Ala Tyr Thr Trp Asp Ser Gly Glu Gln Tyr Leu
610 615 620
Phe Lys Ser Thr Ile Ala Tyr Ala Met Ile Lys Tyr Tyr Ser Glu Val
625 630 635 640
Lys Ser Glu Gln Val Pro Phe Thr Ala Glu Asn Val Phe Val Thr Arg
645 650 655
Glu Thr Leu Arg Ile Ser Phe Tyr Phe His Val Thr Asp Pro Arg Asn
660 665 670
Ile Ser Ser Phe Ile Pro Lys Ile Asp Val Glu Asp Ala Val Arg Leu
675 680 685
Ser Arg Gly Arg Ile Asn Ser Ala Phe Asn Leu Asp Asp Asn Thr Leu
690 695 700
Glu Phe Val Asp Ile Leu Ser Thr Leu Ser Pro Ser Val Glu Pro Pro
705 710 715 720
Val Thr
<210> 78
<211> 597
<212> PRT
<213> Artificial Sequence
<220>
<223> sACE2-615（蝾螈）氨基酸序列
<400> 78
Asp Val Thr Asn Asp Ala Arg Val Phe Leu Asp Ala Phe Asn Ala Gln
1 5 10 15
Ala Glu Asp Leu Ser Tyr Glu Asn Ser Leu Ala Ser Trp Ala Tyr Asn
20 25 30
Thr Asn Ile Thr Glu Glu Asn Ala Ile Lys Met Asn Glu Ala Gly Ala
35 40 45
Lys Trp Thr Ala Phe Tyr Lys Lys Ala Asn Asn Asn Ala Ser Arg Phe
50 55 60
Pro Val Asp Gln Ile Thr Asp Pro Asp Ile Lys Leu Gln Ile Leu Ser
65 70 75 80
Leu Gly Glu Lys Gly Ser Ser Val Leu Pro Asp Asp Lys Tyr Asn Arg
85 90 95
Leu Asn Lys Ala Leu Ser Asp Met Ser Thr Ile Tyr Ser Thr Gly Thr
100 105 110
Val Cys Asp Asn Ser Ala Lys Cys Leu Gln Leu Glu Pro Gly Leu Asp
115 120 125
Leu Ile Met Ala Asp Ser Thr Asp Tyr His Lys Arg Leu Trp Ala Trp
130 135 140
Glu Gly Trp Arg Ser Glu Val Gly Lys Lys Met Arg Pro Leu Tyr Glu
145 150 155 160
Thr Tyr Val Asp Leu Asn Asn Glu Ala Ala Lys Leu Asn Asp Tyr Ala
165 170 175
Asp Tyr Gly Asp Tyr Trp Arg Gly Asn Tyr Glu Thr Gln Asp Ser Gly
180 185 190
Lys Tyr Ala Tyr Ser Arg Asn Asp Leu Lys Arg Asp Val Glu Arg Thr
195 200 205
Phe Lys Glu Ile Gln Pro Leu Tyr Arg Glu Leu His Ala Tyr Val Arg
210 215 220
Asp Lys Leu Arg Gly Val Tyr Gly Asp Lys Tyr Ile Ser Lys Asn Gly
225 230 235 240
Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe Trp Thr
245 250 255
Asn Leu Tyr Pro Leu Ala Val Pro Tyr Pro Asn Gln Pro Ser Ile Asp
260 265 270
Val Thr Ser Ala Met Asn Ala Lys Lys Trp Asn Val Asp Lys Met Phe
275 280 285
Arg Glu Ala Glu Asp Phe Phe Val Ser Val Gly Leu Tyr Lys Met Asn
290 295 300
Glu Asn Phe Trp Asn Phe Ser Met Leu Thr Glu Pro Asn Asp Gly Arg
305 310 315 320
Asn Val Val Cys His Pro Thr Ala Trp Asp Met Gly Lys Asn Asp Phe
325 330 335
Arg Ile Lys Met Cys Thr Lys Val Asn Met Glu Asp Phe Leu Thr Val
340 345 350
His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala Asn Leu
355 360 365
Ser Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu Ala Val
370 375 380
Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu Lys Ser
385 390 395 400
Leu Asp Leu Leu Pro Pro Thr Phe Val Glu Asn Glu Glu Thr Asn Ile
405 410 415
Asn Phe Leu Leu Arg Gln Ala Leu Thr Ile Val Ala Thr Met Pro Phe
420 425 430
Thr Tyr Met Leu Glu Glu Trp Arg Trp Lys Val Phe Asn Gly Glu Ile
435 440 445
Pro Arg Asp Gln Trp Met Lys Lys Trp Trp Gln Met Lys Arg Glu Ile
450 455 460
Val Gly Val Met Glu Pro Val Pro His Asp Glu Thr Tyr Cys Asp Pro
465 470 475 480
Ala Ala Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile Arg Tyr Tyr
485 490 495
Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys Lys Ala
500 505 510
Ala Asn His Asn Gly Ser Leu His Thr Cys Asp Ile Thr Asn Ser Thr
515 520 525
Leu Ala Gly Gln Lys Leu Arg Thr Met Leu Ala Leu Gly Asn Ser Lys
530 535 540
Pro Trp Thr Met Ala Leu Glu Ser Ile Thr Gly Gly Lys Thr Met Asp
545 550 555 560
Ala Gln Pro Leu Leu His Tyr Phe Asp Pro Leu Tyr Thr Trp Leu Arg
565 570 575
Lys Asn Asn Ile Asp Asn Asn Arg Gln Thr Tyr Trp Asp Thr Glu Trp
580 585 590
Ser Ala Tyr Thr Asp
595
<210> 79
<211> 625
<212> PRT
<213> Artificial Sequence
<220>
<223> SsACE2-740（野猪）氨基酸序列
<400> 79
Met Asn Lys Met Ser Ser Ile Tyr Ser Thr Gly Thr Val Cys Lys Arg
1 5 10 15
Glu Asp Pro Phe Asp Cys Gln Thr Leu Glu Pro Gly Leu Glu Ser Val
20 25 30
Met Ala Asn Met Asp Ser Asp Tyr Tyr Glu Arg Leu His Val Trp Glu
35 40 45
Gly Trp Arg Val Glu Val Gly Lys Lys Met Arg Pro Leu Tyr Glu Asp
50 55 60
Tyr Val Asp Leu Lys Asn Glu Ala Ala Lys Leu Asn Gly Tyr Glu Asp
65 70 75 80
Tyr Gly Asp Tyr Trp Arg Ser Asn Tyr Glu Thr Ile Asp Asp Ser Pro
85 90 95
Tyr Asn Tyr Ala Arg Gly Gln Leu Met Thr Asp Val Arg His Ile Tyr
100 105 110
Lys Glu Ile Leu Pro Leu Tyr Lys Glu Leu His Ala Tyr Val Arg Ser
115 120 125
Lys Leu Gln Ala Lys His Pro Glu His Ile His Pro Glu Gly Gly Leu
130 135 140
Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe Trp Thr Gly Leu
145 150 155 160
Tyr Pro Ile Ser Thr Pro Phe Pro Glu Lys Thr Asp Ile Asp Val Thr
165 170 175
Asp Ala Met Ile Ala Gln Lys Trp Pro Lys Asp Arg Leu Phe Gln Glu
180 185 190
Ala Glu Lys Phe Phe Met Ser Val Gly Leu Tyr Lys Met Phe Asp Asn
195 200 205
Phe Trp Lys Asp Ser Met Leu Glu Lys Pro Thr Asp Gly Arg Lys Val
210 215 220
Val Cys His Pro Thr Ala Trp Asp Met Gly Asn Arg Glu Asp Phe Arg
225 230 235 240
Ile Lys Met Cys Thr Glu Val Asn Met Asp His Phe Leu Thr Ala His
245 250 255
His Glu Met Gly His Asn Gln Tyr Gln Met Ala Tyr Arg Asn Leu Ser
260 265 270
Tyr Leu Leu Arg Asp Gly Ala Asn Glu Gly Phe His Glu Ala Val Gly
275 280 285
Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu Lys Ala Leu
290 295 300
Gly Leu Leu Pro Asp Asp Phe Val Glu Asp Lys Glu Thr Glu Ile Asn
305 310 315 320
Phe Leu Met Lys Gln Ala Leu Thr Ile Val Ala Thr Leu Pro Phe Thr
325 330 335
Tyr Met Leu Glu Glu Trp Arg Trp Gln Val Phe Leu Gly Thr Ile Pro
340 345 350
Lys Asp Gln Trp Met Gln Arg Trp Trp Glu Met Lys Arg Asp Met Val
355 360 365
Gly Val Val Glu Pro Leu Pro Arg Asp Glu Thr Tyr Cys Asp Pro Pro
370 375 380
Ala Leu Phe His Val Ser Gly Asp Tyr Ser Phe Ile Arg Tyr Phe Thr
385 390 395 400
Arg Thr Ile Tyr Gln Phe Gln Phe Gln Lys Ala Leu Cys Glu Ala Ala
405 410 415
Gly His Ser Gly Pro Leu Phe Lys Cys Asp Ile Thr Asn Ser Thr Ala
420 425 430
Ala Gly Asp Lys Leu Arg Thr Met Leu Glu Phe Gly Arg Ser Lys Ser
435 440 445
Trp Thr Arg Ala Leu Glu Thr Ile Ser Gly His Ala Lys Met Asp Ser
450 455 460
Ala Pro Leu Leu Asp Tyr Phe Lys Asp Leu His Val Trp Leu Ile Glu
465 470 475 480
Glu Asn Arg Lys Asn Asn Arg Lys Pro Gly Trp Arg Ala Ala Glu Asp
485 490 495
Pro Phe Ser Glu Asn Ala Tyr Lys Val Arg Leu Ser Leu Lys Ala Ala
500 505 510
Met Gly Asp Lys Ala Tyr Ile Trp Asn Ala Asn Glu Met Tyr Leu Phe
515 520 525
Lys Ala Asn Met Ala Tyr Ala Met Arg Gln Tyr Tyr Leu Glu Val Asn
530 535 540
Lys Thr Glu Val Leu Phe Thr Thr Glu Asn Ile His Thr Tyr Lys Glu
545 550 555 560
Thr Ala Arg Ile Ser Phe Tyr Phe Val Val Thr Asp Pro Ala Asn Pro
565 570 575
Ala Val Val Ile Pro Lys Ala Glu Val Glu Ala Ala Ile Arg Leu Ser
580 585 590
Arg Gly Arg Ile Asn Asp Ala Phe Lys Leu Asp Asp Lys Thr Leu Glu
595 600 605
Phe Glu Gly Leu Leu Ala Thr Leu Ala Pro Pro Val Glu Gln Pro Val
610 615 620
Thr
625
<210> 80
<211> 597
<212> PRT
<213> Artificial Sequence
<220>
<223> SsACE2-615（野猪）氨基酸序列
<400> 80
Ser Thr Thr Glu Glu Leu Ala Lys Thr Phe Leu Glu Lys Phe Asn Leu
1 5 10 15
Glu Ala Glu Asp Leu Ala Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr
20 25 30
Asn Thr Asn Ile Thr Asp Glu Asn Ile Gln Lys Met Asn Asp Ala Arg
35 40 45
Ala Lys Trp Ser Ala Phe Tyr Glu Glu Gln Ser Arg Ile Ala Lys Thr
50 55 60
Tyr Pro Leu Asp Glu Ile Gln Thr Leu Ile Leu Lys Arg Gln Leu Gln
65 70 75 80
Ala Leu Gln Gln Ser Gly Thr Ser Gly Leu Ser Ala Asp Lys Ser Lys
85 90 95
Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Ser Gly
100 105 110
Lys Val Leu Asp Pro Asn Asn Pro Gln Glu Cys Leu Val Leu Glu Pro
115 120 125
Gly Leu Asp Glu Ile Met Glu Asn Ser Lys Asp Tyr Ser Arg Arg Leu
130 135 140
Trp Ala Trp Glu Ser Trp Arg Ala Glu Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Val Leu Glu Asn Glu Met Ala Arg Ala Asn
165 170 175
Asn Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Val Thr
180 185 190
Gly Thr Gly Asp Tyr Asp Tyr Ser Arg Asn Gln Leu Met Glu Asp Val
195 200 205
Glu Arg Thr Phe Ala Glu Ile Lys Pro Leu Tyr Glu His Leu His Ala
210 215 220
Tyr Val Arg Ala Lys Leu Met Asp Ala Tyr Pro Ser Arg Ile Ser Pro
225 230 235 240
Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Pro Leu Thr Val Pro Phe Gly Glu Lys Pro Ser
260 265 270
Ile Asp Val Thr Glu Ala Met Val Asn Gln Ser Trp Asp Ala Ile Arg
275 280 285
Ile Phe Glu Glu Ala Glu Lys Phe Phe Val Ser Ile Gly Leu Pro Asn
290 295 300
Met Thr Gln Gly Phe Trp Asn Asn Ser Met Leu Thr Glu Pro Gly Asp
305 310 315 320
Gly Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly
325 330 335
Asp Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala
355 360 365
Ile Gln Pro Tyr Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro His Tyr Leu
385 390 395 400
Lys Ala Leu Gly Leu Leu Pro Pro Asp Phe Tyr Glu Asp Ser Glu Thr
405 410 415
Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly
435 440 445
Glu Ile Pro Lys Glu Gln Trp Met Gln Lys Trp Trp Glu Met Lys Arg
450 455 460
Glu Ile Val Gly Val Val Glu Pro Leu Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Cys Leu Phe His Val Ala Glu Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe His Glu Ala Leu Cys
500 505 510
Arg Thr Ala Lys His Glu Gly Pro Leu Tyr Lys Cys Asp Ile Ser Asn
515 520 525
Ser Thr Glu Ala Gly Gln Lys Leu Leu Gln Met Leu Ser Leu Gly Lys
530 535 540
Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Ile Val Gly Val Lys Thr
545 550 555 560
Met Asp Val Lys Pro Leu Leu Ser Tyr Phe Glu Pro Leu Leu Thr Trp
565 570 575
Leu Lys Ala Gln Asn Gly Asn Ser Ser Val Gly Trp Asn Thr Asp Trp
580 585 590
Thr Pro Tyr Ala Asp
595
<210> 81
<211> 719
<212> PRT
<213> Artificial Sequence
<220>
<223> TeACE2-740（蛇）氨基酸序列
<400> 81
Asp Val Thr Gln Gln Ala Ala Glu Phe Leu Lys Gln Phe Asp Ala Arg
1 5 10 15
Ala Asp Asp Leu Tyr Tyr Ala Ala Ser Ile Ala Ser Trp Asn Tyr Asn
20 25 30
Thr Asn Leu Thr Glu Glu Asn Ala Lys Ile Met His Glu Lys Asp Asn
35 40 45
Ile Phe Ser Lys Phe Tyr Glu Glu Ala Ser Lys Asn Ala Ser Met Tyr
50 55 60
Asn Val Asn Gln Ile Thr Asn Glu Thr Ile Arg Leu Gln Leu His Leu
65 70 75 80
Leu Gln Asn Val Pro Thr Asn Ser Ser Thr Lys Asp Gln Leu Asp Thr
85 90 95
Val Leu Arg Lys Met Ser Thr Met Tyr Ser Thr Gly Thr Val Cys Lys
100 105 110
Gln Asp Asp Pro Phe Asn Cys Leu Pro Leu Glu Pro Gly Leu Asp Asp
115 120 125
Ile Met Glu Asn Asn Trp Ser Tyr Ser Glu Arg Leu Trp Ala Trp Glu
130 135 140
Gly Trp Arg Ala Asp Val Gly Lys Lys Met Arg Pro Leu Tyr Glu Ser
145 150 155 160
Tyr Val Glu Leu Lys Asn Lys Tyr Ala Arg Leu Arg Gly Tyr Ala Asp
165 170 175
Tyr Gly Asp Tyr Trp Arg Ala Asn Tyr Glu Val Asp Leu Pro Lys Glu
180 185 190
Tyr Gln Tyr Gln Arg Ala Gln Leu Ile Thr Asp Val Glu Asn Thr Leu
195 200 205
Gln Gln Ile Met Pro Leu Tyr Lys His Leu His Ala Tyr Val Arg Arg
210 215 220
His Leu Tyr Lys His Tyr Gly Pro Glu Phe Ile Asn Leu Glu Gly Ala
225 230 235 240
Ile Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe Trp Thr Asn
245 250 255
Leu Tyr Pro Leu Met Val Pro Phe Pro Asn Lys Thr Ser Ile Asp Val
260 265 270
Thr Ser Ala Met Val Thr Lys Lys Trp Thr Val Asn Ser Ile Phe Lys
275 280 285
Ala Ala Glu Gln Phe Phe Thr Ser Ile Gly Leu Phe Pro Met Thr Asp
290 295 300
Asn Phe Trp Asn Asn Ser Met Leu Glu Glu Pro Lys Asp Gly Arg Lys
305 310 315 320
Val Val Cys His Pro Thr Ala Trp Asp Met Gly Lys Lys Asp Tyr Arg
325 330 335
Ile Lys Met Cys Thr Lys Ile Asn Met Glu Asp Phe Leu Thr Ala His
340 345 350
His Glu Met Gly His Ile Glu Tyr Asp Met Ala Tyr Ser Asp Gln Pro
355 360 365
Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu Ala Val Gly
370 375 380
Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys Tyr Leu Lys Ser Leu
385 390 395 400
Gly Leu Leu Glu His Thr Phe Gln Glu Asp Thr Glu Thr Asp Ile Asn
405 410 415
Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Met Pro Phe Thr
420 425 430
Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Ala Glu Gln Ile Pro
435 440 445
Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met Lys Arg Glu Ile Val
450 455 460
Gly Val Val Glu Pro Leu Pro His Asn Glu Glu Tyr Cys Asp Pro Ala
465 470 475 480
Ala Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile Arg Tyr Tyr Thr
485 490 495
Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys Gln Ala Ala
500 505 510
Gly His Thr Gly Glu Leu Tyr Lys Cys Glu Ile Ser His Ser Thr Asp
515 520 525
Ala Gly His Ile Leu Lys Asp Met Leu Ala Leu Gly Ser Ser Gln Pro
530 535 540
Trp Thr Lys Ala Leu Glu Ser Ile Thr Lys Ser Gln Lys Met Asp Ala
545 550 555 560
Thr Pro Phe Arg His Tyr Phe Asp Pro Leu Leu Lys Trp Leu Glu Lys
565 570 575
Gln Asn Ser Asn Glu Asn Val Gly Trp Asn Val Asn Trp Thr Pro Tyr
580 585 590
Ser Lys Tyr Ala Ile Lys Val Arg Ile Ser Leu Lys Arg Ala Leu Gly
595 600 605
Asp Asp Ala Tyr Asn Trp Thr Ala Ser Glu Met Tyr Leu Phe Lys Ser
610 615 620
Thr Ile Ala Tyr Ala Met Gln Lys Tyr Phe Leu Glu Ile Lys Asn Lys
625 630 635 640
Thr Val Leu Phe Gln Thr Asp Asn Val His Val Ser Pro Val Thr Glu
645 650 655
Arg Ile Ser Phe Tyr Phe Thr Val Ser Met Pro Thr Asn Ile Ser Glu
660 665 670
Leu Val Pro Lys Ser Glu Val Glu Glu Ala Ile Ser Leu Ser Arg Asp
675 680 685
Arg Ile Asn Glu Ala Phe Arg Leu Thr Asp Gln Thr Leu Glu Phe Val
690 695 700
Gly Leu Leu Pro Thr Leu Ala Pro Pro Tyr Glu Ser Pro Ile Thr
705 710 715
<210> 82
<211> 594
<212> PRT
<213> Artificial Sequence
<220>
<223> TeACE2-615（蛇）氨基酸序列
<400> 82
Asp Val Thr Gln Gln Ala Ala Glu Phe Leu Lys Gln Phe Asp Ala Arg
1 5 10 15
Ala Asp Asp Leu Tyr Tyr Ala Ala Ser Ile Ala Ser Trp Asn Tyr Asn
20 25 30
Thr Asn Leu Thr Glu Glu Asn Ala Lys Ile Met His Glu Lys Asp Asn
35 40 45
Ile Phe Ser Lys Phe Tyr Glu Glu Ala Ser Lys Asn Ala Ser Met Tyr
50 55 60
Asn Val Asn Gln Ile Thr Asn Glu Thr Ile Arg Leu Gln Leu His Leu
65 70 75 80
Leu Gln Asn Val Pro Thr Asn Ser Ser Thr Lys Asp Gln Leu Asp Thr
85 90 95
Val Leu Arg Lys Met Ser Thr Met Tyr Ser Thr Gly Thr Val Cys Lys
100 105 110
Gln Asp Asp Pro Phe Asn Cys Leu Pro Leu Glu Pro Gly Leu Asp Asp
115 120 125
Ile Met Glu Asn Asn Trp Ser Tyr Ser Glu Arg Leu Trp Ala Trp Glu
130 135 140
Gly Trp Arg Ala Asp Val Gly Lys Lys Met Arg Pro Leu Tyr Glu Ser
145 150 155 160
Tyr Val Glu Leu Lys Asn Lys Tyr Ala Arg Leu Arg Gly Tyr Ala Asp
165 170 175
Tyr Gly Asp Tyr Trp Arg Ala Asn Tyr Glu Val Asp Leu Pro Lys Glu
180 185 190
Tyr Gln Tyr Gln Arg Ala Gln Leu Ile Thr Asp Val Glu Asn Thr Leu
195 200 205
Gln Gln Ile Met Pro Leu Tyr Lys His Leu His Ala Tyr Val Arg Arg
210 215 220
His Leu Tyr Lys His Tyr Gly Pro Glu Phe Ile Asn Leu Glu Gly Ala
225 230 235 240
Ile Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe Trp Thr Asn
245 250 255
Leu Tyr Pro Leu Met Val Pro Phe Pro Asn Lys Thr Ser Ile Asp Val
260 265 270
Thr Ser Ala Met Val Thr Lys Lys Trp Thr Val Asn Ser Ile Phe Lys
275 280 285
Ala Ala Glu Gln Phe Phe Thr Ser Ile Gly Leu Phe Pro Met Thr Asp
290 295 300
Asn Phe Trp Asn Asn Ser Met Leu Glu Glu Pro Lys Asp Gly Arg Lys
305 310 315 320
Val Val Cys His Pro Thr Ala Trp Asp Met Gly Lys Lys Asp Tyr Arg
325 330 335
Ile Lys Met Cys Thr Lys Ile Asn Met Glu Asp Phe Leu Thr Ala His
340 345 350
His Glu Met Gly His Ile Glu Tyr Asp Met Ala Tyr Ser Asp Gln Pro
355 360 365
Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu Ala Val Gly
370 375 380
Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys Tyr Leu Lys Ser Leu
385 390 395 400
Gly Leu Leu Glu His Thr Phe Gln Glu Asp Thr Glu Thr Asp Ile Asn
405 410 415
Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Met Pro Phe Thr
420 425 430
Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Ala Glu Gln Ile Pro
435 440 445
Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met Lys Arg Glu Ile Val
450 455 460
Gly Val Val Glu Pro Leu Pro His Asn Glu Glu Tyr Cys Asp Pro Ala
465 470 475 480
Ala Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile Arg Tyr Tyr Thr
485 490 495
Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys Gln Ala Ala
500 505 510
Gly His Thr Gly Glu Leu Tyr Lys Cys Glu Ile Ser His Ser Thr Asp
515 520 525
Ala Gly His Ile Leu Lys Asp Met Leu Ala Leu Gly Ser Ser Gln Pro
530 535 540
Trp Thr Lys Ala Leu Glu Ser Ile Thr Lys Ser Gln Lys Met Asp Ala
545 550 555 560
Thr Pro Phe Arg His Tyr Phe Asp Pro Leu Leu Lys Trp Leu Glu Lys
565 570 575
Gln Asn Ser Asn Glu Asn Val Gly Trp Asn Val Asn Trp Thr Pro Tyr
580 585 590
Ser Lys
<210> 83
<211> 726
<212> PRT
<213> Artificial Sequence
<220>
<223> CsACE2-740（银鲑鱼）氨基酸序列
<400> 83
Ser Asp Leu Glu Arg Arg Ala Gln Glu Phe Leu Asn Gln Phe Asp Gly
1 5 10 15
Asn Ala Thr His Leu Met Tyr Gln Tyr Ser Leu Ala Ser Trp Ala Tyr
20 25 30
Asn Thr Asp Ile Ser Gln Glu Asn Leu Asp Lys Leu Gly Val Gln Ser
35 40 45
Ala Ile Trp Gly Glu Tyr Tyr Ser Thr Val Ser Lys Glu Ser Glu Lys
50 55 60
Phe Pro Ile Asp Gln Ile Arg Asp Pro Leu Ile Lys Leu Gln Leu Ile
65 70 75 80
Ser Leu Gln Asp Lys Gly Ser Gly Ala Leu Ser Ala Asp Lys Ala Ala
85 90 95
His Leu Asn Lys Val Met Asn Glu Met Ser Ser Ile Tyr Ser Thr Gly
100 105 110
Thr Val Cys Lys Arg Glu Asp Pro Phe Asp Cys Gln Thr Leu Glu Pro
115 120 125
Gly Leu Glu Ser Val Met Ala Asn Met Asp Ser Asp Tyr Tyr Glu Arg
130 135 140
Leu His Val Trp Glu Gly Trp Arg Val Glu Val Gly Lys Lys Met Arg
145 150 155 160
Pro Leu Tyr Glu Asp Tyr Val Asp Leu Lys Asn Glu Ala Ala Lys Leu
165 170 175
Asn Asp Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Ser Asn Tyr Glu Thr
180 185 190
Thr Asp Asp Ser Pro Tyr Asn Tyr Ala Arg Gly Gln Leu Met Thr Asp
195 200 205
Val Arg Arg Ile Tyr Lys Glu Ile Leu Pro Leu Tyr Lys Glu Leu His
210 215 220
Ala Tyr Val Arg Ser Lys Leu Gln Ala Lys His Pro Glu His Ile His
225 230 235 240
Pro Glu Gly Gly Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg
245 250 255
Phe Trp Thr Gly Leu Tyr Pro Ile Ser Thr Pro Phe Pro Glu Lys Ile
260 265 270
Asp Ile Asp Val Thr Asn Ala Met Ile Ala Gln Lys Trp Pro Lys Asp
275 280 285
Arg Leu Phe Gln Glu Ala Glu Lys Phe Phe Met Ser Val Gly Leu Tyr
290 295 300
Lys Met Phe Asp Asn Phe Trp Lys Asp Ser Met Leu Glu Lys Pro Thr
305 310 315 320
Asp Gly Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Met Gly Asn
325 330 335
Arg Glu Asp Phe Arg Ile Lys Met Cys Thr Glu Val Asn Met Asp His
340 345 350
Phe Leu Thr Ala His His Glu Met Gly His Asn Gln Tyr Gln Met Ala
355 360 365
Tyr Arg Asn Leu Ser Tyr Leu Leu Arg Asp Gly Ala Asn Glu Gly Phe
370 375 380
His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys
385 390 395 400
His Leu Lys Ala Leu Gly Leu Leu Pro Asp Asp Phe Val Glu Asp Lys
405 410 415
Glu Thr Glu Ile Asn Phe Leu Met Lys Gln Ala Leu Thr Ile Val Ala
420 425 430
Thr Leu Pro Phe Thr Tyr Met Leu Glu Glu Trp Arg Trp Gln Val Phe
435 440 445
Leu Gly Thr Ile Pro Lys Asp Gln Trp Met Gln Arg Trp Trp Glu Met
450 455 460
Lys Arg Asp Met Val Gly Val Val Glu Pro Leu Pro Arg Asp Glu Thr
465 470 475 480
Tyr Cys Asp Pro Pro Ala Leu Phe His Val Ser Gly Asp Tyr Ser Phe
485 490 495
Ile Arg Tyr Phe Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Lys Ala
500 505 510
Leu Cys Glu Ala Ala Gly His Ser Gly Pro Leu Phe Lys Cys Asp Ile
515 520 525
Thr Asn Ser Thr Ala Ala Gly Asp Lys Leu Arg Thr Met Leu Glu Phe
530 535 540
Gly Arg Ser Lys Ser Trp Thr Arg Ala Leu Glu Thr Ile Ser Gly Asn
545 550 555 560
Pro Lys Met Asp Ser Ala Pro Leu Leu Asp Tyr Phe Lys Asp Leu His
565 570 575
Val Trp Leu Leu Glu Glu Asn Arg Lys Asn Asn Arg Lys Pro Gly Trp
580 585 590
Lys Ala Ala Glu Asp Pro Phe Ser Glu Asn Ala Tyr Lys Val Arg Leu
595 600 605
Ser Leu Lys Ala Ala Met Gly Asp Lys Ala Tyr Lys Trp Asn Ala Asn
610 615 620
Glu Met Tyr Leu Phe Lys Ala Asn Met Ala Tyr Ala Met Arg Gln Tyr
625 630 635 640
Tyr Leu Glu Val Asn Lys Thr Ala Ala Leu Phe Thr Thr Glu Asn Ile
645 650 655
His Thr Tyr Lys Glu Thr Ala Arg Ile Ser Phe Tyr Phe Val Val Thr
660 665 670
Asp Pro Ala Asn Ser Ala Val Val Ile Pro Lys Ala Glu Val Glu Ala
675 680 685
Ala Ile Arg Met Ser Arg Gly Arg Ile Asn Asp Ala Phe Lys Leu Asp
690 695 700
Asp Lys Thr Leu Glu Phe Glu Gly Leu Leu Ala Thr Leu Ala Pro Pro
705 710 715 720
Val Glu Gln Pro Val Thr
725
<210> 84
<211> 601
<212> PRT
<213> Artificial Sequence
<220>
<223> CsACE2-615（银鲑鱼）氨基酸序列
<400> 84
Ser Asp Leu Glu Arg Arg Ala Gln Glu Phe Leu Asn Gln Phe Asp Gly
1 5 10 15
Asn Ala Thr His Leu Met Tyr Gln Tyr Ser Leu Ala Ser Trp Ala Tyr
20 25 30
Asn Thr Asp Ile Ser Gln Glu Asn Leu Asp Lys Leu Gly Val Gln Ser
35 40 45
Ala Ile Trp Gly Glu Tyr Tyr Ser Thr Val Ser Lys Glu Ser Glu Lys
50 55 60
Phe Pro Ile Asp Gln Ile Arg Asp Pro Leu Ile Lys Leu Gln Leu Ile
65 70 75 80
Ser Leu Gln Asp Lys Gly Ser Gly Ala Leu Ser Ala Asp Lys Ala Ala
85 90 95
His Leu Asn Lys Val Met Asn Glu Met Ser Ser Ile Tyr Ser Thr Gly
100 105 110
Thr Val Cys Lys Arg Glu Asp Pro Phe Asp Cys Gln Thr Leu Glu Pro
115 120 125
Gly Leu Glu Ser Val Met Ala Asn Met Asp Ser Asp Tyr Tyr Glu Arg
130 135 140
Leu His Val Trp Glu Gly Trp Arg Val Glu Val Gly Lys Lys Met Arg
145 150 155 160
Pro Leu Tyr Glu Asp Tyr Val Asp Leu Lys Asn Glu Ala Ala Lys Leu
165 170 175
Asn Asp Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Ser Asn Tyr Glu Thr
180 185 190
Thr Asp Asp Ser Pro Tyr Asn Tyr Ala Arg Gly Gln Leu Met Thr Asp
195 200 205
Val Arg Arg Ile Tyr Lys Glu Ile Leu Pro Leu Tyr Lys Glu Leu His
210 215 220
Ala Tyr Val Arg Ser Lys Leu Gln Ala Lys His Pro Glu His Ile His
225 230 235 240
Pro Glu Gly Gly Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg
245 250 255
Phe Trp Thr Gly Leu Tyr Pro Ile Ser Thr Pro Phe Pro Glu Lys Ile
260 265 270
Asp Ile Asp Val Thr Asn Ala Met Ile Ala Gln Lys Trp Pro Lys Asp
275 280 285
Arg Leu Phe Gln Glu Ala Glu Lys Phe Phe Met Ser Val Gly Leu Tyr
290 295 300
Lys Met Phe Asp Asn Phe Trp Lys Asp Ser Met Leu Glu Lys Pro Thr
305 310 315 320
Asp Gly Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Met Gly Asn
325 330 335
Arg Glu Asp Phe Arg Ile Lys Met Cys Thr Glu Val Asn Met Asp His
340 345 350
Phe Leu Thr Ala His His Glu Met Gly His Asn Gln Tyr Gln Met Ala
355 360 365
Tyr Arg Asn Leu Ser Tyr Leu Leu Arg Asp Gly Ala Asn Glu Gly Phe
370 375 380
His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys
385 390 395 400
His Leu Lys Ala Leu Gly Leu Leu Pro Asp Asp Phe Val Glu Asp Lys
405 410 415
Glu Thr Glu Ile Asn Phe Leu Met Lys Gln Ala Leu Thr Ile Val Ala
420 425 430
Thr Leu Pro Phe Thr Tyr Met Leu Glu Glu Trp Arg Trp Gln Val Phe
435 440 445
Leu Gly Thr Ile Pro Lys Asp Gln Trp Met Gln Arg Trp Trp Glu Met
450 455 460
Lys Arg Asp Met Val Gly Val Val Glu Pro Leu Pro Arg Asp Glu Thr
465 470 475 480
Tyr Cys Asp Pro Pro Ala Leu Phe His Val Ser Gly Asp Tyr Ser Phe
485 490 495
Ile Arg Tyr Phe Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Lys Ala
500 505 510
Leu Cys Glu Ala Ala Gly His Ser Gly Pro Leu Phe Lys Cys Asp Ile
515 520 525
Thr Asn Ser Thr Ala Ala Gly Asp Lys Leu Arg Thr Met Leu Glu Phe
530 535 540
Gly Arg Ser Lys Ser Trp Thr Arg Ala Leu Glu Thr Ile Ser Gly Asn
545 550 555 560
Pro Lys Met Asp Ser Ala Pro Leu Leu Asp Tyr Phe Lys Asp Leu His
565 570 575
Val Trp Leu Leu Glu Glu Asn Arg Lys Asn Asn Arg Lys Pro Gly Trp
580 585 590
Lys Ala Ala Glu Asp Pro Phe Ser Glu
595 600
<210> 85
<211> 726
<212> PRT
<213> Artificial Sequence
<220>
<223> RtACE2-740（虹鳟鱼）氨基酸序列
<400> 85
Ser Asp Leu Glu Arg Arg Ala Gln Glu Phe Leu Asp Gln Phe Asp Gly
1 5 10 15
Asn Ala Thr His Leu Met Tyr Gln Tyr Ser Leu Ala Ser Trp Ala Tyr
20 25 30
Asn Thr Asp Ile Ser Gln Glu Asn Leu Asp Lys Leu Gly Val Gln Ser
35 40 45
Thr Ile Trp Gly Glu Tyr Tyr Ser Thr Val Ser Lys Glu Ser Glu Lys
50 55 60
Phe Pro Ile Asp Gln Ile Ser Asp Pro Leu Ile Arg Leu Gln Leu Ile
65 70 75 80
Ser Leu Gln Asp Lys Gly Ser Gly Ala Leu Ser Ala Asp Lys Ala Ala
85 90 95
His Leu Asn Lys Val Met Asn Glu Met Ser Ser Ile Tyr Ser Thr Gly
100 105 110
Thr Val Cys Lys Arg Glu Asp Pro Leu Asp Cys Gln Thr Leu Glu Pro
115 120 125
Gly Leu Glu Ser Val Met Ala Asn Met Asp Ser Asp Tyr Tyr Glu Arg
130 135 140
Leu His Val Trp Glu Gly Trp Arg Val Glu Val Gly Lys Lys Met Arg
145 150 155 160
Pro Leu Tyr Glu Asp Tyr Val Asp Leu Lys Asn Glu Ala Ala Lys Leu
165 170 175
Asn Asp Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Ser Asn Tyr Glu Thr
180 185 190
Ile Asp Asp Ser Pro Tyr Asn Tyr Ala Arg Gly Gln Leu Met Thr Asp
195 200 205
Val Arg Arg Ile Tyr Lys Glu Ile Leu Pro Leu Tyr Lys Glu Leu His
210 215 220
Ala Tyr Val Arg Ser Lys Leu Gln Ala Lys His Pro Glu His Ile His
225 230 235 240
Pro Glu Gly Gly Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg
245 250 255
Phe Trp Thr Gly Leu Tyr Pro Ile Ser Thr Pro Phe Pro Glu Lys Thr
260 265 270
Asp Ile Asp Val Thr Glu Ala Met Ile Ala Gln Lys Trp Pro Lys Asp
275 280 285
Arg Leu Phe Gln Glu Ala Glu Lys Phe Phe Met Ser Val Gly Leu Tyr
290 295 300
Lys Met Phe Asp Asn Phe Trp Lys Asp Ser Met Leu Glu Lys Pro Thr
305 310 315 320
Asp Gly Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Met Gly Asn
325 330 335
Arg Glu Asp Phe Arg Ile Lys Met Cys Thr Glu Val Asn Met Asp His
340 345 350
Phe Leu Thr Ala His His Glu Met Gly His Asn Gln Tyr Gln Met Ala
355 360 365
Tyr Arg Asn Leu Ser Tyr Leu Leu Arg Asp Gly Ala Asn Glu Gly Phe
370 375 380
His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys
385 390 395 400
His Leu Lys Ala Leu Gly Leu Leu Pro Gly Asp Phe Val Glu Asp Lys
405 410 415
Glu Thr Glu Ile Asn Phe Leu Met Lys Gln Ala Leu Thr Ile Val Ala
420 425 430
Thr Leu Pro Phe Thr Tyr Met Leu Glu Glu Trp Arg Trp Gln Val Phe
435 440 445
Leu Gly Thr Ile Pro Lys Asp Gln Trp Met Gln Arg Trp Trp Glu Met
450 455 460
Lys Arg Asp Met Val Gly Val Val Glu Pro Leu Pro Arg Asp Glu Thr
465 470 475 480
Tyr Cys Asp Pro Pro Ala Leu Phe His Val Ser Gly Asp Tyr Ser Phe
485 490 495
Ile Arg Tyr Phe Thr Arg Thr Val Tyr Gln Phe Gln Phe Gln Lys Ala
500 505 510
Leu Cys Glu Ala Ala Gly His Ser Gly Pro Leu Phe Lys Cys Asp Ile
515 520 525
Thr Asn Ser Thr Ala Ala Gly Asp Lys Leu Arg Thr Met Leu Glu Phe
530 535 540
Gly Arg Ser Lys Ser Trp Thr Arg Ala Leu Glu Thr Ile Ser Gly Asn
545 550 555 560
Ala Lys Met Asp Ser Ala Pro Leu Leu Asp Tyr Phe Lys Asp Leu His
565 570 575
Val Trp Leu Ile Glu Glu Asn Arg Lys Asn Asn Arg Lys Pro Gly Trp
580 585 590
Arg Ala Ala Glu Asp Pro Phe Ser Ala Asn Ala Tyr Lys Val Arg Leu
595 600 605
Ser Leu Lys Ala Ala Met Gly Asp Lys Ala Tyr Met Trp Asn Ala Asn
610 615 620
Glu Met Tyr Leu Phe Lys Ala Asn Met Ala Tyr Ala Met Arg Gln Tyr
625 630 635 640
Tyr Leu Glu Val Asn Lys Thr Ala Ala Leu Phe Thr Thr Glu Asn Ile
645 650 655
His Thr Tyr Lys Glu Thr Ala Arg Ile Ser Phe Tyr Phe Val Val Thr
660 665 670
Asp Pro Ala Asn Ser Ala Val Val Ile Pro Lys Ala Glu Val Glu Ala
675 680 685
Ala Ile Arg Met Ser Arg Gly Arg Ile Asn Asp Ala Phe Lys Leu Asp
690 695 700
Asp Lys Thr Leu Glu Phe Glu Gly Leu Leu Ala Thr Leu Ala Pro Pro
705 710 715 720
Val Glu Gln Pro Val Thr
725
<210> 86
<211> 601
<212> PRT
<213> Artificial Sequence
<220>
<223> RtACE2-615（虹鳟鱼）氨基酸序列
<400> 86
Ser Asp Leu Glu Arg Arg Ala Gln Glu Phe Leu Asp Gln Phe Asp Gly
1 5 10 15
Asn Ala Thr His Leu Met Tyr Gln Tyr Ser Leu Ala Ser Trp Ala Tyr
20 25 30
Asn Thr Asp Ile Ser Gln Glu Asn Leu Asp Lys Leu Gly Val Gln Ser
35 40 45
Thr Ile Trp Gly Glu Tyr Tyr Ser Thr Val Ser Lys Glu Ser Glu Lys
50 55 60
Phe Pro Ile Asp Gln Ile Ser Asp Pro Leu Ile Arg Leu Gln Leu Ile
65 70 75 80
Ser Leu Gln Asp Lys Gly Ser Gly Ala Leu Ser Ala Asp Lys Ala Ala
85 90 95
His Leu Asn Lys Val Met Asn Glu Met Ser Ser Ile Tyr Ser Thr Gly
100 105 110
Thr Val Cys Lys Arg Glu Asp Pro Leu Asp Cys Gln Thr Leu Glu Pro
115 120 125
Gly Leu Glu Ser Val Met Ala Asn Met Asp Ser Asp Tyr Tyr Glu Arg
130 135 140
Leu His Val Trp Glu Gly Trp Arg Val Glu Val Gly Lys Lys Met Arg
145 150 155 160
Pro Leu Tyr Glu Asp Tyr Val Asp Leu Lys Asn Glu Ala Ala Lys Leu
165 170 175
Asn Asp Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Ser Asn Tyr Glu Thr
180 185 190
Ile Asp Asp Ser Pro Tyr Asn Tyr Ala Arg Gly Gln Leu Met Thr Asp
195 200 205
Val Arg Arg Ile Tyr Lys Glu Ile Leu Pro Leu Tyr Lys Glu Leu His
210 215 220
Ala Tyr Val Arg Ser Lys Leu Gln Ala Lys His Pro Glu His Ile His
225 230 235 240
Pro Glu Gly Gly Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg
245 250 255
Phe Trp Thr Gly Leu Tyr Pro Ile Ser Thr Pro Phe Pro Glu Lys Thr
260 265 270
Asp Ile Asp Val Thr Glu Ala Met Ile Ala Gln Lys Trp Pro Lys Asp
275 280 285
Arg Leu Phe Gln Glu Ala Glu Lys Phe Phe Met Ser Val Gly Leu Tyr
290 295 300
Lys Met Phe Asp Asn Phe Trp Lys Asp Ser Met Leu Glu Lys Pro Thr
305 310 315 320
Asp Gly Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Met Gly Asn
325 330 335
Arg Glu Asp Phe Arg Ile Lys Met Cys Thr Glu Val Asn Met Asp His
340 345 350
Phe Leu Thr Ala His His Glu Met Gly His Asn Gln Tyr Gln Met Ala
355 360 365
Tyr Arg Asn Leu Ser Tyr Leu Leu Arg Asp Gly Ala Asn Glu Gly Phe
370 375 380
His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys
385 390 395 400
His Leu Lys Ala Leu Gly Leu Leu Pro Gly Asp Phe Val Glu Asp Lys
405 410 415
Glu Thr Glu Ile Asn Phe Leu Met Lys Gln Ala Leu Thr Ile Val Ala
420 425 430
Thr Leu Pro Phe Thr Tyr Met Leu Glu Glu Trp Arg Trp Gln Val Phe
435 440 445
Leu Gly Thr Ile Pro Lys Asp Gln Trp Met Gln Arg Trp Trp Glu Met
450 455 460
Lys Arg Asp Met Val Gly Val Val Glu Pro Leu Pro Arg Asp Glu Thr
465 470 475 480
Tyr Cys Asp Pro Pro Ala Leu Phe His Val Ser Gly Asp Tyr Ser Phe
485 490 495
Ile Arg Tyr Phe Thr Arg Thr Val Tyr Gln Phe Gln Phe Gln Lys Ala
500 505 510
Leu Cys Glu Ala Ala Gly His Ser Gly Pro Leu Phe Lys Cys Asp Ile
515 520 525
Thr Asn Ser Thr Ala Ala Gly Asp Lys Leu Arg Thr Met Leu Glu Phe
530 535 540
Gly Arg Ser Lys Ser Trp Thr Arg Ala Leu Glu Thr Ile Ser Gly Asn
545 550 555 560
Ala Lys Met Asp Ser Ala Pro Leu Leu Asp Tyr Phe Lys Asp Leu His
565 570 575
Val Trp Leu Ile Glu Glu Asn Arg Lys Asn Asn Arg Lys Pro Gly Trp
580 585 590
Arg Ala Ala Glu Asp Pro Phe Ser Ala
595 600
<210> 87
<211> 685
<212> PRT
<213> Artificial Sequence
<220>
<223> SalACE2-740（三文鱼）氨基酸序列
<400> 87
Met Asn Lys Met Ser Ser Ile Tyr Ser Thr Gly Thr Val Cys Lys Arg
1 5 10 15
Glu Asp Pro Phe Asp Cys Gln Thr Leu Glu Pro Gly Leu Glu Ser Val
20 25 30
Met Ala Asn Met Asp Ser Asp Tyr Tyr Glu Arg Leu His Val Trp Glu
35 40 45
Gly Trp Arg Val Glu Val Gly Lys Lys Met Arg Pro Leu Tyr Glu Asp
50 55 60
Tyr Val Asp Leu Lys Asn Glu Ala Ala Lys Leu Asn Gly Tyr Glu Asp
65 70 75 80
Tyr Gly Asp Tyr Trp Arg Ser Asn Tyr Glu Thr Ile Asp Asp Ser Pro
85 90 95
Tyr Asn Tyr Ala Arg Gly Gln Leu Met Thr Asp Val Arg His Ile Tyr
100 105 110
Lys Glu Ile Leu Pro Leu Tyr Lys Glu Leu His Ala Tyr Val Arg Ser
115 120 125
Lys Leu Gln Ala Lys His Pro Glu His Ile His Pro Glu Gly Gly Leu
130 135 140
Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe Trp Thr Gly Leu
145 150 155 160
Tyr Pro Ile Ser Thr Pro Phe Pro Glu Lys Thr Asp Ile Asp Val Thr
165 170 175
Asp Ala Met Ile Ala Gln Lys Trp Pro Lys Asp Arg Leu Phe Gln Glu
180 185 190
Ala Glu Lys Phe Phe Met Ser Val Gly Leu Tyr Lys Met Phe Asp Asn
195 200 205
Phe Trp Lys Asp Ser Met Leu Glu Lys Pro Thr Asp Gly Arg Lys Val
210 215 220
Val Cys His Pro Thr Ala Trp Asp Met Gly Asn Arg Glu Asp Phe Arg
225 230 235 240
Ile Lys Met Cys Thr Glu Val Asn Met Asp His Phe Leu Thr Ala His
245 250 255
His Glu Met Gly His Asn Gln Tyr Gln Met Ala Tyr Arg Asn Leu Ser
260 265 270
Tyr Leu Leu Arg Asp Gly Ala Asn Glu Gly Phe His Glu Ala Val Gly
275 280 285
Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu Lys Ala Leu
290 295 300
Gly Leu Leu Pro Asp Asp Phe Val Glu Asp Lys Glu Thr Glu Ile Asn
305 310 315 320
Phe Leu Met Lys Gln Ala Leu Thr Ile Val Ala Thr Leu Pro Phe Thr
325 330 335
Tyr Met Leu Glu Glu Trp Arg Trp Gln Val Phe Leu Gly Thr Ile Pro
340 345 350
Lys Asp Gln Trp Met Gln Arg Trp Trp Glu Met Lys Arg Asp Met Val
355 360 365
Gly Val Val Glu Pro Leu Pro Arg Asp Glu Thr Tyr Cys Asp Pro Pro
370 375 380
Ala Leu Phe His Val Ser Gly Asp Tyr Ser Phe Ile Arg Tyr Phe Thr
385 390 395 400
Arg Thr Ile Tyr Gln Phe Gln Phe Gln Lys Ala Leu Cys Glu Ala Ala
405 410 415
Gly His Ser Gly Pro Leu Phe Lys Cys Asp Ile Thr Asn Ser Thr Ala
420 425 430
Ala Gly Asp Lys Leu Arg Thr Met Leu Glu Phe Gly Arg Ser Lys Ser
435 440 445
Trp Thr Arg Ala Leu Glu Thr Ile Ser Gly His Ala Lys Met Asp Ser
450 455 460
Ala Pro Leu Leu Asp Tyr Phe Lys Asp Leu His Val Trp Leu Ile Glu
465 470 475 480
Glu Asn Arg Lys Asn Asn Arg Lys Pro Gly Trp Arg Ala Ala Glu Asp
485 490 495
Pro Phe Ser Glu Asn Ala Tyr Lys Val Arg Leu Ser Leu Lys Ala Ala
500 505 510
Met Gly Asp Lys Ala Tyr Ile Trp Asn Ala Asn Glu Met Tyr Leu Phe
515 520 525
Lys Ala Asn Met Ala Tyr Ala Met Arg Gln Tyr Tyr Leu Glu Val Asn
530 535 540
Lys Thr Glu Val Leu Phe Thr Thr Glu Asn Ile His Thr Tyr Lys Glu
545 550 555 560
Thr Ala Arg Ile Ser Phe Tyr Phe Val Val Thr Asp Pro Ala Asn Pro
565 570 575
Ala Val Val Ile Pro Lys Ala Glu Val Glu Ala Ala Ile Arg Leu Ser
580 585 590
Arg Gly Arg Ile Asn Asp Ala Phe Lys Leu Asp Asp Lys Thr Leu Glu
595 600 605
Phe Glu Gly Leu Leu Ala Thr Leu Ala Pro Pro Val Glu Gln Pro Val
610 615 620
Thr Val Trp Leu Val Val Phe Gly Val Val Met Gly Leu Val Val Cys
625 630 635 640
Met Gly Cys Tyr Leu Ile Ile Ser Gly Phe Arg Asp Arg Lys Lys Lys
645 650 655
Cys Ala Ala Lys Ala Lys Glu Asn Ala Glu Asn Pro Tyr Gly Val Thr
660 665 670
Asn Lys Thr Phe Glu Arg Glu Glu Asp Glu Gln Thr Gly
675 680 685
<210> 88
<211> 625
<212> PRT
<213> Artificial Sequence
<220>
<223> SalACE2-615（三文鱼）氨基酸序列
<400> 88
Met Asn Lys Met Ser Ser Ile Tyr Ser Thr Gly Thr Val Cys Lys Arg
1 5 10 15
Glu Asp Pro Phe Asp Cys Gln Thr Leu Glu Pro Gly Leu Glu Ser Val
20 25 30
Met Ala Asn Met Asp Ser Asp Tyr Tyr Glu Arg Leu His Val Trp Glu
35 40 45
Gly Trp Arg Val Glu Val Gly Lys Lys Met Arg Pro Leu Tyr Glu Asp
50 55 60
Tyr Val Asp Leu Lys Asn Glu Ala Ala Lys Leu Asn Gly Tyr Glu Asp
65 70 75 80
Tyr Gly Asp Tyr Trp Arg Ser Asn Tyr Glu Thr Ile Asp Asp Ser Pro
85 90 95
Tyr Asn Tyr Ala Arg Gly Gln Leu Met Thr Asp Val Arg His Ile Tyr
100 105 110
Lys Glu Ile Leu Pro Leu Tyr Lys Glu Leu His Ala Tyr Val Arg Ser
115 120 125
Lys Leu Gln Ala Lys His Pro Glu His Ile His Pro Glu Gly Gly Leu
130 135 140
Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe Trp Thr Gly Leu
145 150 155 160
Tyr Pro Ile Ser Thr Pro Phe Pro Glu Lys Thr Asp Ile Asp Val Thr
165 170 175
Asp Ala Met Ile Ala Gln Lys Trp Pro Lys Asp Arg Leu Phe Gln Glu
180 185 190
Ala Glu Lys Phe Phe Met Ser Val Gly Leu Tyr Lys Met Phe Asp Asn
195 200 205
Phe Trp Lys Asp Ser Met Leu Glu Lys Pro Thr Asp Gly Arg Lys Val
210 215 220
Val Cys His Pro Thr Ala Trp Asp Met Gly Asn Arg Glu Asp Phe Arg
225 230 235 240
Ile Lys Met Cys Thr Glu Val Asn Met Asp His Phe Leu Thr Ala His
245 250 255
His Glu Met Gly His Asn Gln Tyr Gln Met Ala Tyr Arg Asn Leu Ser
260 265 270
Tyr Leu Leu Arg Asp Gly Ala Asn Glu Gly Phe His Glu Ala Val Gly
275 280 285
Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu Lys Ala Leu
290 295 300
Gly Leu Leu Pro Asp Asp Phe Val Glu Asp Lys Glu Thr Glu Ile Asn
305 310 315 320
Phe Leu Met Lys Gln Ala Leu Thr Ile Val Ala Thr Leu Pro Phe Thr
325 330 335
Tyr Met Leu Glu Glu Trp Arg Trp Gln Val Phe Leu Gly Thr Ile Pro
340 345 350
Lys Asp Gln Trp Met Gln Arg Trp Trp Glu Met Lys Arg Asp Met Val
355 360 365
Gly Val Val Glu Pro Leu Pro Arg Asp Glu Thr Tyr Cys Asp Pro Pro
370 375 380
Ala Leu Phe His Val Ser Gly Asp Tyr Ser Phe Ile Arg Tyr Phe Thr
385 390 395 400
Arg Thr Ile Tyr Gln Phe Gln Phe Gln Lys Ala Leu Cys Glu Ala Ala
405 410 415
Gly His Ser Gly Pro Leu Phe Lys Cys Asp Ile Thr Asn Ser Thr Ala
420 425 430
Ala Gly Asp Lys Leu Arg Thr Met Leu Glu Phe Gly Arg Ser Lys Ser
435 440 445
Trp Thr Arg Ala Leu Glu Thr Ile Ser Gly His Ala Lys Met Asp Ser
450 455 460
Ala Pro Leu Leu Asp Tyr Phe Lys Asp Leu His Val Trp Leu Ile Glu
465 470 475 480
Glu Asn Arg Lys Asn Asn Arg Lys Pro Gly Trp Arg Ala Ala Glu Asp
485 490 495
Pro Phe Ser Glu Asn Ala Tyr Lys Val Arg Leu Ser Leu Lys Ala Ala
500 505 510
Met Gly Asp Lys Ala Tyr Ile Trp Asn Ala Asn Glu Met Tyr Leu Phe
515 520 525
Lys Ala Asn Met Ala Tyr Ala Met Arg Gln Tyr Tyr Leu Glu Val Asn
530 535 540
Lys Thr Glu Val Leu Phe Thr Thr Glu Asn Ile His Thr Tyr Lys Glu
545 550 555 560
Thr Ala Arg Ile Ser Phe Tyr Phe Val Val Thr Asp Pro Ala Asn Pro
565 570 575
Ala Val Val Ile Pro Lys Ala Glu Val Glu Ala Ala Ile Arg Leu Ser
580 585 590
Arg Gly Arg Ile Asn Asp Ala Phe Lys Leu Asp Asp Lys Thr Leu Glu
595 600 605
Phe Glu Gly Leu Leu Ala Thr Leu Ala Pro Pro Val Glu Gln Pro Val
610 615 620
Thr
625
<210> 89
<211> 726
<212> PRT
<213> Artificial Sequence
<220>
<223> StACE2-740（亚东鲑）氨基酸序列
<400> 89
Ser Asp Leu Glu Arg Arg Ala Gln Glu Phe Leu Asp Thr Phe Asp Gly
1 5 10 15
Asn Ala Thr His Leu Met Tyr Gln Tyr Ser Leu Ala Ser Trp Ala Tyr
20 25 30
Asn Thr Asp Ile Ser Gln Glu Asn Leu Asp Lys Leu Gly Val Gln Ser
35 40 45
Ala Ile Trp Gly Glu Tyr Tyr Ser Lys Val Ser Lys Glu Ser Glu Asn
50 55 60
Phe Pro Ile Asp Gln Ile Ser Asp Pro Leu Ile Lys Leu Gln Leu Thr
65 70 75 80
Ser Leu Gln Asp Lys Gly Ser Gly Ala Leu Ser Ala Asp Lys Ala Ala
85 90 95
His Leu Asn Lys Val Met Asn Lys Met Ser Ser Ile Tyr Ser Thr Gly
100 105 110
Thr Val Cys Lys Arg Glu Asp Pro Phe Asp Cys Gln Thr Leu Glu Pro
115 120 125
Gly Leu Glu Ser Val Met Ala Asn Met Asp Ser Asp Tyr Tyr Glu Arg
130 135 140
Leu His Val Trp Glu Gly Trp Arg Val Glu Val Gly Lys Lys Met Arg
145 150 155 160
Pro Leu Tyr Glu Asp Tyr Val Asp Leu Lys Asn Glu Ala Ala Lys Leu
165 170 175
Asn Gly Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Ser Asn Tyr Glu Thr
180 185 190
Ile Asp Asp Ser Pro Tyr Asn Tyr Ala Arg Gly Gln Leu Met Thr Asp
195 200 205
Val Arg Arg Ile Tyr Lys Glu Ile Leu Pro Leu Tyr Lys Glu Leu His
210 215 220
Ala Tyr Val Arg Ser Lys Leu Gln Ala Lys His Pro Glu His Ile His
225 230 235 240
Pro Glu Gly Gly Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg
245 250 255
Phe Trp Thr Gly Leu Tyr Pro Ile Ser Thr Pro Phe Pro Glu Lys Thr
260 265 270
Asp Ile Asp Val Thr Asp Ala Met Ile Ala Gln Lys Trp Pro Lys Asp
275 280 285
Arg Leu Phe Gln Glu Ala Glu Lys Phe Phe Met Ser Val Gly Leu Tyr
290 295 300
Lys Met Phe Asp Asn Phe Trp Lys Asp Ser Met Leu Glu Lys Pro Thr
305 310 315 320
Asp Gly Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Met Gly Asn
325 330 335
Arg Glu Asp Phe Arg Ile Lys Met Cys Thr Glu Val Asn Met Asp His
340 345 350
Phe Leu Thr Ala His His Glu Met Gly His Asn Gln Tyr Gln Met Ala
355 360 365
Tyr Arg Asn Leu Ser Tyr Leu Leu Arg Asp Gly Ala Asn Glu Gly Phe
370 375 380
His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys
385 390 395 400
His Leu Lys Ala Leu Gly Leu Leu Pro Asp Asp Phe Val Glu Asp Lys
405 410 415
Glu Thr Glu Ile Asn Phe Leu Met Lys Gln Ala Leu Thr Ile Val Ala
420 425 430
Thr Leu Pro Phe Thr Tyr Met Leu Glu Glu Trp Arg Trp Gln Val Phe
435 440 445
Leu Gly Thr Ile Pro Lys Asp Gln Trp Met Gln Arg Trp Trp Glu Met
450 455 460
Lys Arg Asp Met Val Gly Val Val Glu Pro Leu Pro Arg Asp Glu Thr
465 470 475 480
Tyr Cys Asp Pro Pro Ala Leu Phe His Val Ser Gly Asp Tyr Ser Phe
485 490 495
Ile Arg Tyr Phe Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Lys Ala
500 505 510
Leu Cys Glu Ala Ala Gly His Ser Gly Pro Leu Phe Lys Cys Asp Ile
515 520 525
Thr Asn Ser Thr Ala Ala Gly Asp Lys Leu Arg Thr Met Leu Glu Phe
530 535 540
Gly Arg Ser Lys Ser Trp Thr Arg Ala Leu Glu Thr Ile Ser Gly His
545 550 555 560
Ala Lys Met Asp Ser Ala Pro Leu Leu Asp Tyr Phe Lys Asp Leu His
565 570 575
Val Trp Leu Ile Glu Glu Asn Arg Lys Asn Asn Arg Lys Pro Gly Trp
580 585 590
Arg Ala Ala Glu Asp Pro Phe Ser Glu Asn Ala Tyr Lys Val Arg Leu
595 600 605
Ser Leu Lys Ala Ala Met Gly Asp Lys Ala Tyr Ile Trp Asn Gly Asn
610 615 620
Glu Met Tyr Leu Phe Lys Ala Asn Met Ala Tyr Ala Met Arg Gln Tyr
625 630 635 640
Tyr Leu Glu Val Asn Lys Thr Glu Val Leu Phe Thr Thr Glu Asn Ile
645 650 655
His Thr Tyr Lys Glu Thr Ala Arg Ile Ser Phe Tyr Phe Val Val Thr
660 665 670
Asp Pro Ala Asn Pro Ala Val Val Ile Pro Lys Ala Glu Val Glu Ala
675 680 685
Ala Ile Arg Leu Ser Arg Gly Arg Ile Asn Asp Ala Phe Lys Leu Asp
690 695 700
Asp Lys Thr Leu Glu Phe Glu Gly Leu Leu Ala Thr Leu Ala Pro Pro
705 710 715 720
Val Glu Gln Pro Val Thr
725
<210> 90
<211> 601
<212> PRT
<213> Artificial Sequence
<220>
<223> StACE2-615（亚东鲑）氨基酸序列
<400> 90
Ser Asp Leu Glu Arg Arg Ala Gln Glu Phe Leu Asp Thr Phe Asp Gly
1 5 10 15
Asn Ala Thr His Leu Met Tyr Gln Tyr Ser Leu Ala Ser Trp Ala Tyr
20 25 30
Asn Thr Asp Ile Ser Gln Glu Asn Leu Asp Lys Leu Gly Val Gln Ser
35 40 45
Ala Ile Trp Gly Glu Tyr Tyr Ser Lys Val Ser Lys Glu Ser Glu Asn
50 55 60
Phe Pro Ile Asp Gln Ile Ser Asp Pro Leu Ile Lys Leu Gln Leu Thr
65 70 75 80
Ser Leu Gln Asp Lys Gly Ser Gly Ala Leu Ser Ala Asp Lys Ala Ala
85 90 95
His Leu Asn Lys Val Met Asn Lys Met Ser Ser Ile Tyr Ser Thr Gly
100 105 110
Thr Val Cys Lys Arg Glu Asp Pro Phe Asp Cys Gln Thr Leu Glu Pro
115 120 125
Gly Leu Glu Ser Val Met Ala Asn Met Asp Ser Asp Tyr Tyr Glu Arg
130 135 140
Leu His Val Trp Glu Gly Trp Arg Val Glu Val Gly Lys Lys Met Arg
145 150 155 160
Pro Leu Tyr Glu Asp Tyr Val Asp Leu Lys Asn Glu Ala Ala Lys Leu
165 170 175
Asn Gly Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Ser Asn Tyr Glu Thr
180 185 190
Ile Asp Asp Ser Pro Tyr Asn Tyr Ala Arg Gly Gln Leu Met Thr Asp
195 200 205
Val Arg Arg Ile Tyr Lys Glu Ile Leu Pro Leu Tyr Lys Glu Leu His
210 215 220
Ala Tyr Val Arg Ser Lys Leu Gln Ala Lys His Pro Glu His Ile His
225 230 235 240
Pro Glu Gly Gly Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg
245 250 255
Phe Trp Thr Gly Leu Tyr Pro Ile Ser Thr Pro Phe Pro Glu Lys Thr
260 265 270
Asp Ile Asp Val Thr Asp Ala Met Ile Ala Gln Lys Trp Pro Lys Asp
275 280 285
Arg Leu Phe Gln Glu Ala Glu Lys Phe Phe Met Ser Val Gly Leu Tyr
290 295 300
Lys Met Phe Asp Asn Phe Trp Lys Asp Ser Met Leu Glu Lys Pro Thr
305 310 315 320
Asp Gly Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Met Gly Asn
325 330 335
Arg Glu Asp Phe Arg Ile Lys Met Cys Thr Glu Val Asn Met Asp His
340 345 350
Phe Leu Thr Ala His His Glu Met Gly His Asn Gln Tyr Gln Met Ala
355 360 365
Tyr Arg Asn Leu Ser Tyr Leu Leu Arg Asp Gly Ala Asn Glu Gly Phe
370 375 380
His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys
385 390 395 400
His Leu Lys Ala Leu Gly Leu Leu Pro Asp Asp Phe Val Glu Asp Lys
405 410 415
Glu Thr Glu Ile Asn Phe Leu Met Lys Gln Ala Leu Thr Ile Val Ala
420 425 430
Thr Leu Pro Phe Thr Tyr Met Leu Glu Glu Trp Arg Trp Gln Val Phe
435 440 445
Leu Gly Thr Ile Pro Lys Asp Gln Trp Met Gln Arg Trp Trp Glu Met
450 455 460
Lys Arg Asp Met Val Gly Val Val Glu Pro Leu Pro Arg Asp Glu Thr
465 470 475 480
Tyr Cys Asp Pro Pro Ala Leu Phe His Val Ser Gly Asp Tyr Ser Phe
485 490 495
Ile Arg Tyr Phe Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Lys Ala
500 505 510
Leu Cys Glu Ala Ala Gly His Ser Gly Pro Leu Phe Lys Cys Asp Ile
515 520 525
Thr Asn Ser Thr Ala Ala Gly Asp Lys Leu Arg Thr Met Leu Glu Phe
530 535 540
Gly Arg Ser Lys Ser Trp Thr Arg Ala Leu Glu Thr Ile Ser Gly His
545 550 555 560
Ala Lys Met Asp Ser Ala Pro Leu Leu Asp Tyr Phe Lys Asp Leu His
565 570 575
Val Trp Leu Ile Glu Glu Asn Arg Lys Asn Asn Arg Lys Pro Gly Trp
580 585 590
Arg Ala Ala Glu Asp Pro Phe Ser Glu
595 600
<210> 91
<211> 723
<212> PRT
<213> Artificial Sequence
<220>
<223> MlACE2-740（水貂）氨基酸序列
<400> 91
Gln Ser Thr Thr Glu Asp Leu Ala Lys Thr Phe Leu Glu Lys Phe Asn
1 5 10 15
Tyr Glu Ala Glu Glu Leu Ser Tyr Gln Asn Ser Leu Ala Ser Trp Asn
20 25 30
Tyr Asn Thr Asn Ile Thr Asp Glu Asn Ile Gln Lys Met Asn Ile Ala
35 40 45
Gly Ala Lys Trp Ser Ala Phe Tyr Glu Glu Glu Ser Gln His Ala Lys
50 55 60
Thr Tyr Pro Leu Glu Glu Ile Gln Asp Pro Ile Ile Lys Arg Gln Leu
65 70 75 80
Arg Ala Leu Gln Gln Ser Gly Ser Ser Val Leu Ser Ala Asp Lys Arg
85 90 95
Glu Arg Leu Asn Thr Ile Leu Asn Ala Met Ser Thr Ile Tyr Ser Thr
100 105 110
Gly Lys Ala Cys Asn Pro Asn Asn Pro Gln Glu Cys Leu Leu Leu Glu
115 120 125
Pro Gly Leu Asp Asp Ile Met Glu Asn Ser Lys Asp Tyr Asn Glu Arg
130 135 140
Leu Trp Ala Trp Glu Gly Trp Arg Ser Glu Val Gly Lys Gln Leu Arg
145 150 155 160
Pro Leu Tyr Glu Glu Tyr Val Ala Leu Lys Asn Glu Met Ala Arg Ala
165 170 175
Asn Asn Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Glu
180 185 190
Glu Trp Ala Asp Gly Tyr Ser Tyr Ser Arg Asn Gln Leu Ile Glu Asp
195 200 205
Val Glu His Thr Phe Thr Gln Ile Lys Pro Leu Tyr Glu His Leu His
210 215 220
Ala Tyr Val Arg Ala Lys Leu Met Asp Ala Tyr Pro Ser Arg Ile Ser
225 230 235 240
Pro Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg
245 250 255
Phe Trp Thr Asn Leu Tyr Pro Leu Met Val Pro Phe Gly Gln Lys Pro
260 265 270
Asn Ile Asp Val Thr Asp Ala Met Val Asn Gln Ser Trp Asp Ala Arg
275 280 285
Arg Ile Phe Glu Glu Ala Glu Thr Phe Phe Val Ser Val Gly Leu Pro
290 295 300
Asn Met Thr Glu Gly Phe Trp Gln Asn Ser Met Leu Thr Glu Pro Gly
305 310 315 320
Asp Asn Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys
325 330 335
Arg Asp Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asp Phe
340 345 350
Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr
355 360 365
Ala Glu Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His
370 375 380
Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Asn His
385 390 395 400
Leu Lys Asn Ile Gly Leu Leu Pro Pro Asp Phe Ser Glu Asp Ser Glu
405 410 415
Thr Asp Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr
420 425 430
Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys
435 440 445
Gly Glu Ile Pro Lys Glu Gln Trp Met Gln Lys Trp Trp Glu Met Lys
450 455 460
Arg Asp Ile Val Gly Val Val Glu Pro Leu Pro His Asp Glu Thr Tyr
465 470 475 480
Cys Asp Pro Ala Ala Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile
485 490 495
Arg Tyr Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu
500 505 510
Cys Gln Ile Ala Lys His Glu Gly Pro Leu Tyr Lys Cys Asp Ile Ser
515 520 525
Asn Ser Arg Glu Ala Gly Gln Lys Leu His Glu Met Leu Ser Leu Gly
530 535 540
Arg Ser Lys Pro Trp Thr Phe Ala Leu Glu Arg Val Val Gly Ala Lys
545 550 555 560
Thr Met Asp Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr
565 570 575
Trp Leu Lys Glu Gln Asn Arg Asn Ser Phe Val Gly Trp Asn Thr Asp
580 585 590
Trp Ser Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu Lys
595 600 605
Ser Ala Leu Gly Glu Lys Ala Tyr Glu Trp Asn Asp Asn Glu Met Tyr
610 615 620
Phe Phe Gln Ser Ser Ile Ala Tyr Ala Met Arg Glu Tyr Phe Ser Lys
625 630 635 640
Val Lys Asn Gln Thr Ile Pro Phe Val Gly Lys Asp Val Arg Val Ser
645 650 655
Asp Leu Lys Pro Arg Ile Ser Phe Asn Phe Ile Val Thr Ser Pro Glu
660 665 670
Asn Met Ser Asp Ile Ile Pro Arg Ala Asp Val Glu Glu Ala Ile Arg
675 680 685
Lys Ser Arg Gly Arg Ile Asn Asp Ala Phe Arg Leu Asp Asp Asn Ser
690 695 700
Leu Glu Phe Leu Gly Ile Gln Pro Thr Leu Glu Pro Pro Tyr Gln Pro
705 710 715 720
Pro Val Thr
<210> 92
<211> 598
<212> PRT
<213> Artificial Sequence
<220>
<223> MlACE2-615（水貂）氨基酸序列
<400> 92
Gln Ser Thr Thr Glu Asp Leu Ala Lys Thr Phe Leu Glu Lys Phe Asn
1 5 10 15
Tyr Glu Ala Glu Glu Leu Ser Tyr Gln Asn Ser Leu Ala Ser Trp Asn
20 25 30
Tyr Asn Thr Asn Ile Thr Asp Glu Asn Ile Gln Lys Met Asn Ile Ala
35 40 45
Gly Ala Lys Trp Ser Ala Phe Tyr Glu Glu Glu Ser Gln His Ala Lys
50 55 60
Thr Tyr Pro Leu Glu Glu Ile Gln Asp Pro Ile Ile Lys Arg Gln Leu
65 70 75 80
Arg Ala Leu Gln Gln Ser Gly Ser Ser Val Leu Ser Ala Asp Lys Arg
85 90 95
Glu Arg Leu Asn Thr Ile Leu Asn Ala Met Ser Thr Ile Tyr Ser Thr
100 105 110
Gly Lys Ala Cys Asn Pro Asn Asn Pro Gln Glu Cys Leu Leu Leu Glu
115 120 125
Pro Gly Leu Asp Asp Ile Met Glu Asn Ser Lys Asp Tyr Asn Glu Arg
130 135 140
Leu Trp Ala Trp Glu Gly Trp Arg Ser Glu Val Gly Lys Gln Leu Arg
145 150 155 160
Pro Leu Tyr Glu Glu Tyr Val Ala Leu Lys Asn Glu Met Ala Arg Ala
165 170 175
Asn Asn Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Glu
180 185 190
Glu Trp Ala Asp Gly Tyr Ser Tyr Ser Arg Asn Gln Leu Ile Glu Asp
195 200 205
Val Glu His Thr Phe Thr Gln Ile Lys Pro Leu Tyr Glu His Leu His
210 215 220
Ala Tyr Val Arg Ala Lys Leu Met Asp Ala Tyr Pro Ser Arg Ile Ser
225 230 235 240
Pro Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg
245 250 255
Phe Trp Thr Asn Leu Tyr Pro Leu Met Val Pro Phe Gly Gln Lys Pro
260 265 270
Asn Ile Asp Val Thr Asp Ala Met Val Asn Gln Ser Trp Asp Ala Arg
275 280 285
Arg Ile Phe Glu Glu Ala Glu Thr Phe Phe Val Ser Val Gly Leu Pro
290 295 300
Asn Met Thr Glu Gly Phe Trp Gln Asn Ser Met Leu Thr Glu Pro Gly
305 310 315 320
Asp Asn Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys
325 330 335
Arg Asp Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asp Phe
340 345 350
Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr
355 360 365
Ala Glu Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His
370 375 380
Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Asn His
385 390 395 400
Leu Lys Asn Ile Gly Leu Leu Pro Pro Asp Phe Ser Glu Asp Ser Glu
405 410 415
Thr Asp Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr
420 425 430
Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys
435 440 445
Gly Glu Ile Pro Lys Glu Gln Trp Met Gln Lys Trp Trp Glu Met Lys
450 455 460
Arg Asp Ile Val Gly Val Val Glu Pro Leu Pro His Asp Glu Thr Tyr
465 470 475 480
Cys Asp Pro Ala Ala Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile
485 490 495
Arg Tyr Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu
500 505 510
Cys Gln Ile Ala Lys His Glu Gly Pro Leu Tyr Lys Cys Asp Ile Ser
515 520 525
Asn Ser Arg Glu Ala Gly Gln Lys Leu His Glu Met Leu Ser Leu Gly
530 535 540
Arg Ser Lys Pro Trp Thr Phe Ala Leu Glu Arg Val Val Gly Ala Lys
545 550 555 560
Thr Met Asp Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr
565 570 575
Trp Leu Lys Glu Gln Asn Arg Asn Ser Phe Val Gly Trp Asn Thr Asp
580 585 590
Trp Ser Pro Tyr Ala Asp
595
<210> 93
<211> 723
<212> PRT
<213> Artificial Sequence
<220>
<223> VvACE2-740（狐狸）氨基酸序列
<400> 93
Gln Ser Thr Glu Asp Leu Val Asn Thr Phe Leu Glu Lys Phe Asn Tyr
1 5 10 15
Glu Ala Glu Glu Leu Ser Tyr Gln Ser Ser Leu Ala Ser Trp Asp Tyr
20 25 30
Asn Thr Asn Ile Ser Asp Glu Asn Val Gln Lys Met Asn Asn Ala Gly
35 40 45
Ala Lys Trp Ser Ala Phe Tyr Glu Glu Gln Ser Lys Leu Ala Lys Thr
50 55 60
Tyr Pro Leu Glu Glu Ile Gln Asp Ser Thr Val Lys Arg Gln Leu Arg
65 70 75 80
Ala Leu Gln His Ser Gly Ser Ser Val Leu Ser Ala Asp Lys Asn Gln
85 90 95
Arg Leu Asn Thr Ile Leu Asn Ser Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Ala Cys Asn Pro Ser Asn Pro Gln Glu Cys Leu Leu Leu Glu Pro
115 120 125
Gly Leu Asp Asp Ile Met Glu Asn Ser Lys Asp Tyr Asn Glu Arg Leu
130 135 140
Trp Ala Trp Glu Gly Trp Arg Ser Glu Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Ala Leu Lys Asn Glu Met Ala Arg Ala Asn
165 170 175
Asn Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Glu Glu
180 185 190
Trp Glu Asn Gly Tyr Asn Tyr Ser Arg Asn Gln Leu Ile Asp Asp Val
195 200 205
Glu His Thr Phe Thr Gln Ile Met Pro Leu Tyr Gln His Leu His Ala
210 215 220
Tyr Val Arg Thr Lys Leu Met Asp Thr Tyr Pro Ser Tyr Ile Ser Pro
225 230 235 240
Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Pro Leu Thr Val Pro Phe Gly Gln Lys Pro Asn
260 265 270
Ile Asp Val Thr Asn Ala Met Val Asn Gln Ser Trp Asp Ala Arg Lys
275 280 285
Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn
290 295 300
Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Glu Pro Ser Asp
305 310 315 320
Ser Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly
325 330 335
Asp Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala
355 360 365
Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Asn His Leu
385 390 395 400
Lys Asn Ile Gly Leu Leu Pro Pro Ser Phe Phe Glu Asp Ser Glu Thr
405 410 415
Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly
435 440 445
Glu Ile Pro Lys Asp Gln Trp Met Lys Thr Trp Trp Glu Met Lys Arg
450 455 460
Asn Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ser Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Gln Ile Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser Asn
515 520 525
Ser Ser Glu Ala Gly Gln Lys Leu Leu Glu Met Leu Lys Leu Gly Lys
530 535 540
Ser Lys Pro Trp Thr Tyr Ala Leu Glu Ile Val Val Gly Ala Lys Asn
545 550 555 560
Met Asp Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr Trp
565 570 575
Leu Lys Glu Gln Asn Arg Asn Ser Phe Val Gly Trp Asn Thr Asp Trp
580 585 590
Ser Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu Lys Ser
595 600 605
Ala Leu Gly Glu Lys Ala Tyr Glu Trp Asn Asn Asn Glu Met Tyr Leu
610 615 620
Phe Arg Ser Ser Ile Ala Tyr Ala Met Arg Arg Tyr Phe Ser Glu Val
625 630 635 640
Lys Lys Gln Thr Ile Pro Phe Val Glu Asp Asn Val Trp Val Ser Asp
645 650 655
Leu Lys Pro Arg Ile Ser Phe Asn Phe Phe Val Thr Ser Pro Gly Asn
660 665 670
Val Ser Asp Ile Ile Pro Arg Thr Glu Val Glu Lys Ala Ile Arg Met
675 680 685
Tyr Arg Gly Arg Ile Asn Asp Val Phe Arg Leu Asp Asp Asn Ser Leu
690 695 700
Glu Phe Leu Gly Ile Gln Pro Thr Leu Gly Pro Ser Tyr Glu Pro Pro
705 710 715 720
Val Thr Ile
<210> 94
<211> 597
<212> PRT
<213> Artificial Sequence
<220>
<223> VvACE2-615（狐狸）氨基酸序列
<400> 94
Gln Ser Thr Glu Asp Leu Val Asn Thr Phe Leu Glu Lys Phe Asn Tyr
1 5 10 15
Glu Ala Glu Glu Leu Ser Tyr Gln Ser Ser Leu Ala Ser Trp Asp Tyr
20 25 30
Asn Thr Asn Ile Ser Asp Glu Asn Val Gln Lys Met Asn Asn Ala Gly
35 40 45
Ala Lys Trp Ser Ala Phe Tyr Glu Glu Gln Ser Lys Leu Ala Lys Thr
50 55 60
Tyr Pro Leu Glu Glu Ile Gln Asp Ser Thr Val Lys Arg Gln Leu Arg
65 70 75 80
Ala Leu Gln His Ser Gly Ser Ser Val Leu Ser Ala Asp Lys Asn Gln
85 90 95
Arg Leu Asn Thr Ile Leu Asn Ser Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Ala Cys Asn Pro Ser Asn Pro Gln Glu Cys Leu Leu Leu Glu Pro
115 120 125
Gly Leu Asp Asp Ile Met Glu Asn Ser Lys Asp Tyr Asn Glu Arg Leu
130 135 140
Trp Ala Trp Glu Gly Trp Arg Ser Glu Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Ala Leu Lys Asn Glu Met Ala Arg Ala Asn
165 170 175
Asn Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Glu Glu
180 185 190
Trp Glu Asn Gly Tyr Asn Tyr Ser Arg Asn Gln Leu Ile Asp Asp Val
195 200 205
Glu His Thr Phe Thr Gln Ile Met Pro Leu Tyr Gln His Leu His Ala
210 215 220
Tyr Val Arg Thr Lys Leu Met Asp Thr Tyr Pro Ser Tyr Ile Ser Pro
225 230 235 240
Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Pro Leu Thr Val Pro Phe Gly Gln Lys Pro Asn
260 265 270
Ile Asp Val Thr Asn Ala Met Val Asn Gln Ser Trp Asp Ala Arg Lys
275 280 285
Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn
290 295 300
Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Glu Pro Ser Asp
305 310 315 320
Ser Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly
325 330 335
Asp Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala
355 360 365
Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Asn His Leu
385 390 395 400
Lys Asn Ile Gly Leu Leu Pro Pro Ser Phe Phe Glu Asp Ser Glu Thr
405 410 415
Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly
435 440 445
Glu Ile Pro Lys Asp Gln Trp Met Lys Thr Trp Trp Glu Met Lys Arg
450 455 460
Asn Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ser Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Gln Ile Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser Asn
515 520 525
Ser Ser Glu Ala Gly Gln Lys Leu Leu Glu Met Leu Lys Leu Gly Lys
530 535 540
Ser Lys Pro Trp Thr Tyr Ala Leu Glu Ile Val Val Gly Ala Lys Asn
545 550 555 560
Met Asp Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr Trp
565 570 575
Leu Lys Glu Gln Asn Arg Asn Ser Phe Val Gly Trp Asn Thr Asp Trp
580 585 590
Ser Pro Tyr Ala Asp
595
<210> 95
<211> 724
<212> PRT
<213> Artificial Sequence
<220>
<223> EcACE2-740（马）氨基酸序列
<400> 95
Gln Ser Thr Thr Glu Asp Leu Ala Lys Thr Phe Leu Glu Lys Phe Asn
1 5 10 15
Ser Glu Ala Glu Glu Leu Ser His Gln Ser Ser Leu Ala Ser Trp Ser
20 25 30
Tyr Asn Thr Asn Ile Thr Asp Glu Asn Val Gln Lys Met Asn Glu Ala
35 40 45
Gly Ala Arg Trp Ser Ala Phe Tyr Glu Glu Gln Cys Lys Leu Ala Lys
50 55 60
Thr Tyr Pro Leu Glu Glu Ile Gln Asn Leu Thr Val Lys Arg Gln Leu
65 70 75 80
Gln Ala Leu Gln Gln Ser Gly Ser Ser Val Leu Ser Ala Asp Lys Ser
85 90 95
Lys Arg Leu Asn Glu Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr
100 105 110
Gly Lys Val Cys Asn Pro Ser Asn Pro Gln Glu Cys Leu Leu Leu Glu
115 120 125
Pro Gly Leu Asp Ala Ile Met Glu Asn Ser Lys Asp Tyr Asn Gln Arg
130 135 140
Leu Trp Ala Trp Glu Gly Trp Arg Ser Glu Val Gly Lys Gln Leu Arg
145 150 155 160
Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Ala
165 170 175
Asn Asn Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Ala
180 185 190
Glu Gly Pro Ser Gly Tyr Asp Tyr Ser Arg Asp Gln Leu Ile Glu Asp
195 200 205
Val Glu Arg Thr Phe Ala Glu Ile Lys Pro Leu Tyr Glu His Leu His
210 215 220
Ala Tyr Val Arg Ala Lys Leu Met Asp Thr Tyr Pro Ser His Ile Asn
225 230 235 240
Pro Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg
245 250 255
Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys Pro
260 265 270
Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ser Trp Asp Ala Lys
275 280 285
Arg Ile Phe Glu Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro
290 295 300
Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Glu Pro Gly
305 310 315 320
Asp Gly Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys
325 330 335
Gly Asp Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asp Phe
340 345 350
Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr
355 360 365
Ala Val Gln Pro Tyr Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His
370 375 380
Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Asn His
385 390 395 400
Leu Lys Ala Ile Gly Leu Leu Pro Pro Asp Phe Tyr Glu Asp Ser Glu
405 410 415
Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr
420 425 430
Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys
435 440 445
Gly Glu Ile Pro Lys Glu Glu Trp Met Lys Lys Trp Trp Glu Met Lys
450 455 460
Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr
465 470 475 480
Cys Asp Pro Ala Ala Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile
485 490 495
Arg Tyr Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu
500 505 510
Cys Gln Thr Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser
515 520 525
Asn Ser Thr Glu Ala Gly Gln Lys Leu Leu Gln Met Leu Ser Leu Gly
530 535 540
Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Arg Ile Val Gly Val Lys
545 550 555 560
Asn Met Asp Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr
565 570 575
Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr Asn
580 585 590
Trp Ser Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu Lys
595 600 605
Ser Ala Leu Gly Glu Lys Ser Tyr Glu Trp Asn Asp Asn Glu Met Tyr
610 615 620
Leu Phe Gln Ser Ser Val Ala Tyr Ala Met Arg Val Tyr Phe Leu Lys
625 630 635 640
Ala Lys Asn Gln Thr Ile Leu Phe Gly Glu Glu Asp Val Trp Val Ser
645 650 655
Asp Leu Lys Pro Arg Ile Ser Phe Asn Phe Phe Val Thr Ser Pro Lys
660 665 670
Asn Ala Ser Asp Ile Ile Pro Arg Thr Asp Val Glu Glu Ala Ile Arg
675 680 685
Met Ser Arg Ser Arg Ile Asn Asp Ala Phe Arg Leu Asp Asp Asn Thr
690 695 700
Leu Glu Phe Leu Gly Ile Gln Pro Thr Leu Gly Pro Pro Tyr Gln Pro
705 710 715 720
Pro Val Thr Val
<210> 96
<211> 598
<212> PRT
<213> Artificial Sequence
<220>
<223> EcACE2-615（马）氨基酸序列
<400> 96
Gln Ser Thr Thr Glu Asp Leu Ala Lys Thr Phe Leu Glu Lys Phe Asn
1 5 10 15
Ser Glu Ala Glu Glu Leu Ser His Gln Ser Ser Leu Ala Ser Trp Ser
20 25 30
Tyr Asn Thr Asn Ile Thr Asp Glu Asn Val Gln Lys Met Asn Glu Ala
35 40 45
Gly Ala Arg Trp Ser Ala Phe Tyr Glu Glu Gln Cys Lys Leu Ala Lys
50 55 60
Thr Tyr Pro Leu Glu Glu Ile Gln Asn Leu Thr Val Lys Arg Gln Leu
65 70 75 80
Gln Ala Leu Gln Gln Ser Gly Ser Ser Val Leu Ser Ala Asp Lys Ser
85 90 95
Lys Arg Leu Asn Glu Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr
100 105 110
Gly Lys Val Cys Asn Pro Ser Asn Pro Gln Glu Cys Leu Leu Leu Glu
115 120 125
Pro Gly Leu Asp Ala Ile Met Glu Asn Ser Lys Asp Tyr Asn Gln Arg
130 135 140
Leu Trp Ala Trp Glu Gly Trp Arg Ser Glu Val Gly Lys Gln Leu Arg
145 150 155 160
Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Ala
165 170 175
Asn Asn Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Ala
180 185 190
Glu Gly Pro Ser Gly Tyr Asp Tyr Ser Arg Asp Gln Leu Ile Glu Asp
195 200 205
Val Glu Arg Thr Phe Ala Glu Ile Lys Pro Leu Tyr Glu His Leu His
210 215 220
Ala Tyr Val Arg Ala Lys Leu Met Asp Thr Tyr Pro Ser His Ile Asn
225 230 235 240
Pro Thr Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg
245 250 255
Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys Pro
260 265 270
Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ser Trp Asp Ala Lys
275 280 285
Arg Ile Phe Glu Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro
290 295 300
Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Glu Pro Gly
305 310 315 320
Asp Gly Arg Lys Val Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys
325 330 335
Gly Asp Phe Arg Ile Lys Met Cys Thr Lys Val Thr Met Asp Asp Phe
340 345 350
Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr
355 360 365
Ala Val Gln Pro Tyr Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His
370 375 380
Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Asn His
385 390 395 400
Leu Lys Ala Ile Gly Leu Leu Pro Pro Asp Phe Tyr Glu Asp Ser Glu
405 410 415
Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr
420 425 430
Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys
435 440 445
Gly Glu Ile Pro Lys Glu Glu Trp Met Lys Lys Trp Trp Glu Met Lys
450 455 460
Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr
465 470 475 480
Cys Asp Pro Ala Ala Leu Phe His Val Ala Asn Asp Tyr Ser Phe Ile
485 490 495
Arg Tyr Tyr Thr Arg Thr Ile Tyr Gln Phe Gln Phe Gln Glu Ala Leu
500 505 510
Cys Gln Thr Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser
515 520 525
Asn Ser Thr Glu Ala Gly Gln Lys Leu Leu Gln Met Leu Ser Leu Gly
530 535 540
Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Arg Ile Val Gly Val Lys
545 550 555 560
Asn Met Asp Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr
565 570 575
Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr Asn
580 585 590
Trp Ser Pro Tyr Ala Asp
595
<210> 97
<211> 696
<212> DNA
<213> Artificial Sequence
<220>
<223> RBD-StrepII的核酸序列
<400> 97
cgcgtacaac cgacagagtc aattgtacgt tttcctaaca tcaccaatct ctgtccgttt 60
ggtgaagtct ttaacgctac gcggtttgct tccgtttacg cgtggaacag gaaacgaata 120
tcgaactgcg tagctgatta ctccgtgtta tataatagtg cgagcttctc tactttcaag 180
tgttatggtg tttcaccaac aaagttaaat gacctctgct ttaccaacgt atacgccgat 240
agttttgtca taagaggcga cgaggtgagg caaattgcgc ctggacagac agggaaaata 300
gcagattaca attacaaatt gcctgacgat ttcaccggct gtgttatcgc atggaactct 360
aataatctag attctaaggt cggaggcaat tacaattatc tttaccgtct gtttcggaag 420
tccaacttga agccgttcga acgcgacatc tcgacggaga tttatcaagc cggcagcact 480
ccatgtaacg gggttgaggg gttcaactgc tatttccccc tccagtcgta tgggttccag 540
ccaacgaatg gagtcggtta tcaaccctat agagtggtgg tactgtcatt tgaactatta 600
cacgcccctg caacagtttg cggtcccaag aaaagtacta acttggtcaa aaataaactt 660
ccggaaaccg gatggagtca ccctcagttc gagaaa 696
<210> 98
<211> 232
<212> PRT
<213> Artificial Sequence
<220>
<223> RBD-StrepII的氨基酸序列
<400> 98
Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn
1 5 10 15
Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val
20 25 30
Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser
35 40 45
Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val
50 55 60
Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp
65 70 75 80
Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln
85 90 95
Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr
100 105 110
Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly
115 120 125
Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys
130 135 140
Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr
145 150 155 160
Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser
165 170 175
Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val
180 185 190
Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly
195 200 205
Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Leu Pro Glu Thr Gly
210 215 220
Trp Ser His Pro Gln Phe Glu Lys
225 230

Claims (16)

1.一种工程质粒，其包含天然ACE2序列、敲除了跨膜区和胞内区的截短的ACE2序列、或进一步敲除了胞外区的截短ACE2序列作为外源基因。
2.如权利要求1所述的工程质粒，其在如权利要求1所述的ACE2序列的下游还包含标签蛋白的序列，并在其上游包含信号肽序列。
3.如权利要求2所述的工程质粒，其中，所述标签蛋白是HIS或Strep-II，优选HIS。
4.如权利要求3所述的工程质粒，其中，所述工程质粒是pPICZαA，其携带了敲除了跨膜区和胞内区的截短ACE2序列、或进一步敲除了胞外区的截短ACE2序列；
其中表达人的两种截短的ACE2的核酸序列分别是
SEQ ID NO.1、SEQ ID NO.2；
而表达老虎的两种截短的ACE2的核酸序列分别是
SEQ ID NO.3、SEQ ID NO.4；
其中表达牛的两种截短的ACE2的核酸序列分别是
SEQ ID NO.5、SEQ ID NO.6；
而表达斑马鱼的两种截短的ACE2的核酸序列分别是
SEQ ID NO.7、SEQ ID NO.8；
其中表达狗的两种截短的ACE2的核酸序列分别是
SEQ ID NO.9、SEQ ID NO.10；
而表达猫的两种截短的ACE2的核酸序列分别是
SEQ ID NO.11、SEQ ID NO.12；
其中表达雪貂的两种截短的ACE2的核酸序列分别是
SEQ ID NO.13、SEQ ID NO.14；
而表达恒河猴的两种截短的ACE2的核酸序列分别是
SEQ ID NO.15、SEQ ID NO.16；
其中表达穿山甲的两种截短的ACE2的核酸序列分别是
SEQ ID NO.17、SEQ ID NO.18；
而表达土拨鼠的两种截短的ACE2的核酸序列分别是
SEQ ID NO.19、SEQ ID NO.20；
其中表达果子狸的两种截短的ACE2的核酸序列分别是
SEQ ID NO.21、SEQ ID NO.22；
而表达中华鳖的两种截短的ACE2的核酸序列分别是
SEQ ID NO.23、SEQ ID NO.24；
其中表达褐家鼠的两种截短的ACE2的核酸序列分别是
SEQ ID NO.25、SEQ ID NO.26；
而表达马铁菊头蝠的两种截短的ACE2的核酸序列分别是
SEQ ID NO.27、SEQ ID NO.28；
其中表达蝾螈的两种截短的ACE2的核酸序列分别是
SEQ ID NO.29、SEQ ID NO.30；
其中表达野猪的两种截短的ACE2的核酸序列分别是
SEQ ID NO.31、SEQ ID NO.32；
其中表达蛇的两种截短的ACE2的核酸序列分别是
SEQ ID NO.33、SEQ ID NO.34；
其中表达银鲑鱼的两种截短的ACE2的核酸序列分别是
SEQ ID NO.35、SEQ ID NO.36；
其中表达虹鳟鱼的两种截短的ACE2的核酸序列分别是
SEQ ID NO.37、SEQ ID NO.38；
其中表达三文鱼的两种截短的ACE2的核酸序列分别是
SEQ ID NO.39、SEQ ID NO.40；
其中表达亚东鲑的两种截短的ACE2的核酸序列分别是
SEQ ID NO.41、SEQ ID NO.42；
其中表达水貂的两种截短的ACE2的核酸序列分别是
SEQ ID NO.43、SEQ ID NO.44；
其中表达狐狸的两种截短的ACE2的核酸序列分别是
SEQ ID NO.45、SEQ ID NO.46；
其中表达马的两种截短的ACE2的核酸序列分别是
SEQ ID NO.47、SEQ ID NO.48。
5.一种基因工程真核细胞，其包含如权利要求1-4的任一项所述的工程质粒。
6.如权利要求5所述的基因工程真核细胞，其中该基因工程真核细胞改造自巴斯德毕赤酵母(Pichia pastoris)。
7.如权利要求6所述的基因工程真核细胞，其中，所述巴斯德毕赤酵母的菌株为X33株。
8.通过真核细胞发酵法使用如权利要求5-7的任一项所述的基因工程真核细胞生产血管紧张素转化酶2(ACE2)的方法，该方法包括：
-对宿主细胞进行培养，在培养物中表达ACE2；
-从培养物中对ACE2提取纯化。
9.如权利要求8所述的生产方法，其中，所述培养和表达的条件为，种子液30摄氏度下摇床培养20-24h，转入BMMY培养基，30摄氏度下甲醇诱导目标蛋白表达72h；收集发酵液上清。
10.如权利要求8所述的生产方法，其中，所述提取纯化至少使用下述步骤：对发酵液上清进行过滤；使用亲和层析法对ACE2进行提取。
11.一种真核细胞，其染色体上导入有SEQ ID NO.1～SEQ ID NO.48中任一项所示的核酸序列以及SEQ ID NO.97所示的核酸序列；
优选所述真核细胞包含如权利要求1-4的任一项所述的工程质粒以及携带SEQ IDNO.97序列的工程质粒，进一步优选：所述真核细胞包含携带SEQ ID NO.1或2的pPICKαA工程质粒以及携带SEQ ID NO.97序列的pPICKαA工程质粒。
12.如权利要求11所述的真核细胞，其中所述真核细胞为酵母菌，最优选为巴斯德毕赤酵母(Pichia pastoris)。
13.如权利要求12所述的真核细胞，其中，所述巴斯德毕赤酵母的菌株为X33株。
14.通过真核细胞发酵法使用如权利要求11-13的任一项所述的真核细胞共表达血管紧张素转化酶2(ACE2)和新冠棘突蛋白的受体结合域(RBD)的方法，所述方法包括：
-对宿主细胞进行培养，在培养物中共表达ACE2和RBD；
-从培养物中对ACE2和RBD提取纯化。
15.如权利要求14所述的生产方法，其中，所述培养和表达的条件为，种子液30摄氏度下摇床培养20-24h，转入BMMY培养基，30摄氏度下甲醇诱导目标蛋白表达72h；收集发酵液上清。
16.如权利要求14所述的生产方法，其中，所述提取纯化至少使用下述步骤：对发酵液上清进行过滤；使用亲和层析法对ACE2和RBD进行提取。
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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103898148A (zh) * 2014-03-04 2014-07-02 健雄职业技术学院 一种重组质粒pET28a-hACE2及其应用
CN104394878A (zh) * 2012-02-10 2015-03-04 塔瑞克斯制药有限公司 用于治疗周围血管疾病的组合物和方法
US20160376321A1 (en) * 2013-11-26 2016-12-29 Baylor College Of Medicine A novel sars immunogenic composition
CN111474350A (zh) * 2020-04-23 2020-07-31 中国林业科学研究院林业研究所 一种检测冠状病毒s1抗原的试剂盒及其非诊断目的的检测方法
CN111732638A (zh) * 2020-07-02 2020-10-02 重庆博唯佰泰生物制药有限公司 一种针对SARS-CoV-2的疫苗

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104394878A (zh) * 2012-02-10 2015-03-04 塔瑞克斯制药有限公司 用于治疗周围血管疾病的组合物和方法
US20160376321A1 (en) * 2013-11-26 2016-12-29 Baylor College Of Medicine A novel sars immunogenic composition
CN103898148A (zh) * 2014-03-04 2014-07-02 健雄职业技术学院 一种重组质粒pET28a-hACE2及其应用
CN111474350A (zh) * 2020-04-23 2020-07-31 中国林业科学研究院林业研究所 一种检测冠状病毒s1抗原的试剂盒及其非诊断目的的检测方法
CN111732638A (zh) * 2020-07-02 2020-10-02 重庆博唯佰泰生物制药有限公司 一种针对SARS-CoV-2的疫苗

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
徐辉等: "人源血管紧张素转化酶-N结构域基因片段在毕赤酵母中的表达", 《浙江理工大学学报》, vol. 28, no. 4, pages 611 - 615 *

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