CN114685224A - Method for preparing soil conditioner by utilizing wine lees and activated sludge of red wine brewing process - Google Patents

Method for preparing soil conditioner by utilizing wine lees and activated sludge of red wine brewing process Download PDF

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Publication number
CN114685224A
CN114685224A CN202210451420.6A CN202210451420A CN114685224A CN 114685224 A CN114685224 A CN 114685224A CN 202210451420 A CN202210451420 A CN 202210451420A CN 114685224 A CN114685224 A CN 114685224A
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lees
wine
soil conditioner
sludge
brewing process
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景江
王冠龙
刘耀琦
何思雨
罗凌志
蒋婷婷
朱星宇
黄作军
黄泽宇
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Chengdu Technological University CDTU
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Chengdu Technological University CDTU
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/006Waste from chemical processing of material, e.g. diestillation, roasting, cooking
    • C05F5/008Waste from biochemical processing of material, e.g. fermentation, breweries
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C14/00Methods or apparatus for planting not provided for in other groups of this subclass
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C17/00Fertilisers or seeders with centrifugal wheels
    • A01C17/005Driving mechanisms for the throwing devices
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/50Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00Soil-conditioning materials or soil-stabilising materials
    • C09K17/40Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Soil Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Environmental Sciences (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Life Sciences & Earth Sciences (AREA)
  • Materials Engineering (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Inorganic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Botany (AREA)
  • Environmental & Geological Engineering (AREA)
  • Soil Conditioners And Soil-Stabilizing Materials (AREA)
  • Processing Of Solid Wastes (AREA)

Abstract

The invention discloses a method for preparing a soil conditioner by utilizing wine lees and activated sludge of a red wine brewing process, which comprises the following steps: (1) introducing wine lees into a fermentation tank, adding starch, fermenting at 25-35 deg.C for 2-5d, opening the top cover of the fermentation tank, and introducing air at the bottom for blowing to obtain pretreated wine lees; (2) drying the concentrated sludge subjected to the wine making sewage precipitation treatment for 2-5 days to obtain pretreated sludge; (3) and (3) mixing the pretreated wine mud obtained in the step (1) with the pretreated sludge obtained in the step (2), adding wine dregs, fermenting for 25-35 days at the temperature of 25-35 ℃, and keeping ventilation until the water content of a fermented product is lower than 45%, thereby obtaining the nutritional soil conditioner. The invention also discloses the soil conditioner prepared by the method. The method reasonably utilizes the lees, the sludge and the like generated in the wine brewing process, and effectively solves the problems of low resource utilization rate, higher treatment cost and the like in the prior art.

Description

Method for preparing soil conditioner by utilizing wine lees and activated sludge of red wine brewing process
Technical Field
The invention belongs to the technical field of resource utilization, and particularly relates to a method for preparing a soil conditioner by utilizing wine lees and activated sludge of a red wine brewing process.
Background
During the production process of the grape wine, a large amount of waste lees, waste residues, filter residues and the like are generated, and the main components of the waste residues are yeast paste, grape seeds, grape skins and grape stalks; and a large amount of sludge is generated during the treatment of the brewing wastewater. However, for the current wine production enterprises, most of filter residues and the like are treated by garbage, so that reasonable resource utilization is not carried out on one hand, and the added value is very low; on the other hand, certain pollution is generated to the surrounding environment in the stacking process. How to reasonably utilize a large amount of waste materials and waste residues is a common problem which must be faced by the wine making industry, and the traditional disposal mode not only pollutes the environment, seriously threatens the public safety and has higher cost, but also wastes various nutrient components in the grape waste materials and the waste residues.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a method for preparing a soil conditioner by utilizing lees and activated sludge of a red wine brewing process, which reasonably utilizes lees, sludge and the like generated in the wine brewing process, reduces the cost of treatment wastes, realizes resource utilization, and effectively solves the problems of low resource utilization rate, higher treatment cost and the like in the prior art.
In order to achieve the purpose, the technical scheme adopted by the invention for solving the technical problem is as follows: the method for preparing the soil conditioner by utilizing the lees and the activated sludge of the red wine brewing process comprises the following steps:
(1) introducing wine lees after secondary fermentation in wine making into a fermentation tank, adding starch, fermenting at 25-35 deg.C for 2-5 days, opening the top cover of the fermentation tank, introducing air at the bottom, and purging until the water content is less than 80% to obtain pretreated wine lees;
(2) drying the concentrated sludge subjected to the wine making sewage precipitation treatment for 2-5 days until the water content is lower than 85% to obtain pretreated sludge;
(3) and (3) mixing the pretreated wine mud obtained in the step (1) and the pretreated sludge obtained in the step (2) according to the volume ratio of 1-3:5, adding wine residues, fermenting for 25-35d at the temperature of 25-35 ℃, and keeping ventilation until the water content of a fermented product is lower than 45%, the organic matter degradation rate reaches 50%, and the carbon nitrogen ratio is reduced to 8, so as to obtain the nutritional soil conditioner.
Further, the method for preparing the soil conditioner by utilizing the lees and the activated sludge of the red wine brewing process also comprises the following steps:
(4) and (4) adding dry soil in the grape field, fly ash and grape vine powder into the nutritional soil conditioner obtained in the step (3) to obtain the soil covering type soil conditioner.
Furthermore, the addition amounts of the dry soil, the fly ash and the grape vine powder in the grape field are respectively 10-30%, 1-5% and 1-2% of the mass of the soil conditioner.
Further, the addition amounts of the dry soil, the fly ash and the grape vine powder in the grape field are respectively 20%, 3% and 1% of the mass of the soil conditioner.
Further, in the step (1), the adding amount of starch is 0.1-0.2% of the mass of the wine lees.
Further, in the step (1), the adding amount of starch is 0.1% of the mass of the wine lees.
Further, in the step (1), air is introduced at the temperature of 25-35 ℃ for blowing, and the aeration rate is 20-22m3/h。
Further, in the step (1), air is introduced at the temperature of 30 ℃ for blowing, and aeration is carried outThe air volume is 20m3/h。
Further, in the step (3), the addition amount of the wine residues is 8-12% of the total mass of the pre-treated wine mud and the pre-treated sludge.
Further, in the step (3), the addition amount of the wine residues is 10% of the total mass of the pre-treated wine mud and the pre-treated sludge.
Further, in the step (3), the lees are added and fermented for 30 days at the temperature of 30 ℃ and the ventilation is kept.
The nutritional soil conditioner is prepared by the method for preparing the soil conditioner by utilizing the lees and the activated sludge of the red wine brewing process.
The soil conditioner is applied to the grape vine earthing and overwintering.
When the grape vines are covered with soil and overwinter, nutritional soil conditioners are laid firstly after tunnels are dug, then the grape vines are buried, then the soil-covered soil conditioners are laid, and a proper amount of soil is laid on the top layer.
In summary, the invention has the following advantages:
1. the method reasonably utilizes the lees, the sludge and the like generated in the brewing process, reduces the cost of waste treatment, realizes resource utilization, and effectively solves the problems of low resource utilization rate, higher treatment cost and the like in the prior art.
2. The lees are products in the wine brewing process, are waste left at the bottom of the fermentation tank after the second fermentation in the wine brewing process, and mainly comprise lactic acid bacteria; the function of the biological filter is to provide microorganisms and a small amount of organic substances, wherein the microorganisms mainly function to accelerate the dehydration of the mixture; the pressed solid fraction provides mainly organic matter. The sludge is a product after the sewage treatment in the sedimentation tank and mainly consists of microorganisms (bacteria, fungi, protozoa, metazoans, and the like). The lees are products in the brewing process, are waste left at the bottom of the fermentation tank after the first fermentation in brewing wine, and mainly comprise grape skin, yeast and the like. According to the preparation method, the lees, the sludge and the lees are treated and fermented together to prepare the soil conditioner, so that the resource utilization of various wastes in the wine making process is realized, the resource utilization rate is improved, the accumulation or discarding of the wastes is avoided, the environmental pollution is prevented, and the treatment cost is reduced.
3. According to the method, starch is added into the wine lees after secondary fermentation for fermentation, so that the content of microorganisms in the wine lees is increased, then the concentrated sludge is dried, and the sludge, the wine lees and the wine lees are subjected to co-fermentation for comprehensive utilization, the yeast in the wine lees accelerates the dehydration speed of the mixture, and the wine lees and the sludge provide organic matters for fermentation, so that the fermentation process is promoted.
4. The soil conditioner obtained by the invention is used for earthing and overwintering the grapevines and is applied in layers, so that the overwintering of the grapevines is effectively promoted, and the manual application cost is saved.
Detailed Description
Example 1
A soil conditioner prepared from lees and activated sludge of red wine brewing process comprises the following steps:
(1) introducing wine lees after secondary fermentation in wine making into fermentation tank, adding starch, fermenting at 25 deg.C for 2d, opening top cover of the fermentation tank, introducing air at bottom at 25 deg.C for purging, and aerating at 20m3H, obtaining the pre-treated wine mud until the water content is lower than 80%; the adding amount of starch is 0.1 percent of the mass of the wine lees;
(2) drying the concentrated sludge subjected to the wine making sewage precipitation treatment for 2d until the water content is lower than 85% to obtain pretreated sludge;
(3) and (3) mixing the pretreated wine mud obtained in the step (1) and the pretreated sludge obtained in the step (2) according to a volume ratio of 1:5, adding wine residues, fermenting for 25 days at the temperature of 25 ℃, and keeping ventilation, wherein the addition amount of the wine residues is 8% of the total mass of the pretreated wine mud and the pretreated sludge until the water content of a fermented product is lower than 45%, the degradation rate of organic matters reaches 50%, and the carbon-nitrogen ratio is reduced to 8, so that the nutritional soil conditioner is obtained.
Example 2
A soil conditioner prepared from lees and activated sludge of red wine brewing process comprises the following steps:
(1) introducing wine lees after secondary fermentation in wine making into fermentation tank, adding starch, fermenting at 28 deg.C for 3d, and beatingDeveloping a top cover of the fermentation tank, and introducing air at the bottom of the fermentation tank for blowing and sweeping at the temperature of 28 ℃, wherein the aeration rate is 21m3The moisture content is lower than 80 percent to obtain the pre-treated wine mud; the adding amount of starch is 0.1 percent of the mass of the wine lees;
(2) drying the concentrated sludge subjected to the wine making sewage precipitation treatment for 3d until the water content is lower than 85% to obtain pretreated sludge;
(3) and (3) mixing the pretreated wine mud obtained in the step (1) and the pretreated sludge obtained in the step (2) according to a volume ratio of 2:5, adding wine residues, fermenting for 28 days at the temperature of 28 ℃, and keeping ventilation, wherein the addition amount of the wine residues is 9% of the total mass of the pretreated wine mud and the pretreated sludge until the water content of a fermented product is lower than 45%, the degradation rate of organic matters reaches 50%, and the carbon-nitrogen ratio is reduced to 8, so that the nutritional soil conditioner is obtained.
Example 3
A soil conditioner prepared from lees and activated sludge of red wine brewing process comprises the following steps:
(1) introducing wine lees after secondary fermentation in brewing wine into fermentation tank, adding starch, fermenting at 30 deg.C for 3d, opening top cover of fermentation tank, introducing air at bottom at 30 deg.C for purging, and aerating at 20m3The moisture content is lower than 80 percent to obtain the pre-treated wine mud; the adding amount of starch is 0.1 percent of the mass of the wine lees;
(2) drying the concentrated sludge subjected to the wine making sewage precipitation treatment for 3d until the water content is lower than 85% to obtain pretreated sludge;
(3) and (3) mixing the pre-treated wine mud obtained in the step (1) and the pre-treated sludge obtained in the step (2) according to the volume ratio of 2:5, then adding wine residues, fermenting for 30 days at the temperature of 30 ℃, and keeping ventilation, wherein the addition amount of the wine residues is 10% of the total mass of the pre-treated wine mud and the pre-treated sludge until the water content of a fermented product is lower than 45%, the degradation rate of organic matters reaches 50%, and the carbon-nitrogen ratio is reduced to 8, so that the nutritional soil conditioner is obtained.
Example 4
A soil conditioner prepared from lees and activated sludge of red wine brewing process comprises the following steps:
(1) introducing wine lees after secondary fermentation in wine making into fermentation tankAdding starch, fermenting at 35 deg.C for 5 days, opening top cover of the fermentation tank, blowing air at 35 deg.C to purge at bottom, and aerating at 22m3The moisture content is lower than 80 percent to obtain the pre-treated wine mud; the adding amount of starch is 0.2 percent of the mass of the wine lees;
(2) drying the concentrated sludge subjected to the wine making sewage precipitation treatment for 5 days until the water content is lower than 85% to obtain pretreated sludge;
(3) and (3) mixing the pretreated wine mud obtained in the step (1) and the pretreated sludge obtained in the step (2) according to a volume ratio of 3:5, adding wine residues, fermenting for 35d at the temperature of 35 ℃, and keeping ventilation, wherein the addition amount of the wine residues is 12% of the total mass of the pretreated wine mud and the pretreated sludge until the water content of a fermented product is lower than 45%, the degradation rate of organic matters reaches 50%, and the carbon-nitrogen ratio is reduced to 8, so that the nutritional soil conditioner is obtained.
Example 5
A soil conditioner prepared from lees and activated sludge of a red wine brewing process comprises the following steps:
(1) introducing wine lees after secondary fermentation in wine making into fermentation tank, adding starch, fermenting at 30 deg.C for 3d, opening top cover of the fermentation tank, introducing air at bottom at 30 deg.C for purging, and aerating at 20m3H, obtaining the pre-treated wine mud until the water content is lower than 80%; the adding amount of starch is 0.1 percent of the mass of the wine lees;
(2) drying the concentrated sludge subjected to the wine making sewage precipitation treatment for 3d until the water content is lower than 85% to obtain pretreated sludge;
(3) mixing the pre-treated wine mud obtained in the step (1) and the pre-treated sludge obtained in the step (2) according to a volume ratio of 2:5, adding wine residues, fermenting for 30d at the temperature of 30 ℃, and keeping ventilation, wherein the addition amount of the wine residues is 10% of the total mass of the pre-treated wine mud and the pre-treated sludge until the water content of a fermented product is lower than 45%, the degradation rate of organic matters reaches 50%, and the carbon-nitrogen ratio is reduced to 8, so that the nutritional soil conditioner is obtained;
(4) and (4) adding dry soil in the grape field, fly ash and grape vine powder into the nutritional soil conditioner obtained in the step (3) to obtain the soil covering type soil conditioner. The addition amounts of the dry soil, the fly ash and the grape vine powder in the grape field are respectively 20%, 3% and 1% of the mass of the soil conditioner.
Examples of the experiments
In the conventional method, when the grape vine is overwintering, 80% of the grape vine needs to be cut off, and then the rest 20% of grape vine needs to be covered with soil for overwintering. When the nutritional soil conditioner and the soil-covering soil conditioner obtained in the embodiment 4 of the application are used for winter, after the tunnel is dug, the nutritional soil conditioner is laid firstly, then the grape vines are buried, the soil-covering soil conditioner is laid, and a proper amount of soil is laid on the top layer. Indexes such as pruning rate, grape vine survival rate and grape vine biomass of the two methods are measured and calculated, and the results are shown in table 1.
TABLE 1 statistics table of indexes after overwintering
Index (I) Rate of pruning Survival rate of grape vine
Conventional overwintering 80% 89%
Modifier soil covering overwintering 50% 95%
As can be seen from Table 1, the soil conditioner prepared from wine-making wastes such as wine lees, sludge and wine lees reduces the cost of waste treatment, realizes resource utilization, can reduce the pruning rate of grape vines during overwintering, avoids generating more wastes, and can improve the survival rate of the grape vines and the biomass of the grape vines. Not only realizes the resource utilization of the wastes and improves the resource utilization rate, but also effectively promotes the grapevines to pass the winter, saves the manual application cost,
while the present invention has been described in detail with reference to the specific embodiments thereof, it should not be construed as limited by the scope of the present patent. Various modifications and changes may be made by those skilled in the art without inventive work within the scope of the appended claims.

Claims (9)

1. A method for preparing a soil conditioner by utilizing wine lees and activated sludge of a red wine brewing process is characterized by comprising the following steps:
(1) introducing wine lees after secondary fermentation in wine making into a fermentation tank, adding starch, fermenting at 25-35 deg.C for 2-5d, opening the top cover of the fermentation tank, introducing air at the bottom, and purging until the water content is lower than 80% to obtain pretreated wine lees;
(2) drying the concentrated sludge subjected to the wine making sewage precipitation treatment for 2-5 days until the water content is lower than 85% to obtain pretreated sludge;
(3) and (3) mixing the pretreated wine mud obtained in the step (1) and the pretreated sludge obtained in the step (2) according to the volume ratio of 1-3:5, then adding wine lees, fermenting for 25-35d at the temperature of 25-35 ℃, and keeping ventilation until the water content of a fermented product is lower than 45%, the organic matter degradation rate reaches 50%, and the carbon nitrogen ratio is reduced to 8, so as to obtain the nutritional soil conditioner.
2. The method for preparing a soil conditioner using lees and activated sludge of a red wine brewing process according to claim 1, further comprising the steps of:
(4) and (4) adding dry soil in the grape field, fly ash and grape vine powder into the nutritional soil conditioner obtained in the step (3) to obtain the soil covering type soil conditioner.
3. The method for preparing a soil conditioner using lees and activated sludge of a red wine brewing process according to claim 2, wherein the dry soil, fly ash and grape cane powder in the grape field are added in an amount of 10-30%, 1-5% and 1-2% by mass of the soil conditioner, respectively.
4. The method for preparing a soil conditioner using lees and activated sludge of a red wine brewing process according to claim 1, wherein the starch is added in an amount of 0.1 to 0.2% by mass of the lees in the step (1).
5. The method for preparing a soil conditioner using lees and activated sludge of red wine brewing process as set forth in claim 1, wherein in the step (1), air is blown at a temperature of 25-35 ℃ for purging, and the aeration amount is 20-22m3/h。
6. The method for preparing a soil conditioner using lees and activated sludge of a red wine brewing process according to claim 1, wherein in the step (3), the lees is added in an amount of 8 to 12% by mass based on the total mass of the pre-treated lees and the pre-treated sludge.
7. The method for preparing a soil conditioner using lees and activated sludge of red wine brewing process according to claim 1, wherein in the step (3), the lees are added and fermented at 30 ℃ for 30d while keeping aeration.
8. A nutritional soil conditioner obtained by the method for preparing a soil conditioner from lees and activated sludge obtained by the red wine brewing process as set forth in any one of claims 1 to 7.
9. Use of the soil improvement agent of claim 8 for earthing grapevines into the winter.
CN202210451420.6A 2022-04-26 2022-04-26 Method for preparing soil conditioner by utilizing wine lees and activated sludge of red wine brewing process Pending CN114685224A (en)

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Citations (4)

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Publication number Priority date Publication date Assignee Title
CN105585365A (en) * 2016-03-31 2016-05-18 四川沃达丰生物科技有限公司 Microorganism organic fertilizer prepared from sludge and preparing method thereof
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CN110128213A (en) * 2019-06-19 2019-08-16 乐斯福(明光)有限公司 A method of liquid fertilizer is produced using beer waste yeast
CN111825496A (en) * 2020-07-10 2020-10-27 宁夏大学 Method for resource utilization of waste water and waste in wine production

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105585365A (en) * 2016-03-31 2016-05-18 四川沃达丰生物科技有限公司 Microorganism organic fertilizer prepared from sludge and preparing method thereof
CN108623344A (en) * 2018-08-16 2018-10-09 中粮华夏长城葡萄酒有限公司 A kind of organic fertilizer and its preparation method and application
CN110128213A (en) * 2019-06-19 2019-08-16 乐斯福(明光)有限公司 A method of liquid fertilizer is produced using beer waste yeast
CN111825496A (en) * 2020-07-10 2020-10-27 宁夏大学 Method for resource utilization of waste water and waste in wine production

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Title
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翟衡等: "《酿酒葡萄栽培及加工技术》", 30 June 2001, 中国农业出版社, pages: 196 *
贺阳东主编: "《农村生物质综合处理与资源化利用技术》", vol. 1, 31 August 2012, 北京 冶金工业出版社, pages: 104 - 174 *

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