CN114672439A - Multi-base-number regulation and control culture method based on sulfide bacteria - Google Patents
Multi-base-number regulation and control culture method based on sulfide bacteria Download PDFInfo
- Publication number
- CN114672439A CN114672439A CN202210422410.XA CN202210422410A CN114672439A CN 114672439 A CN114672439 A CN 114672439A CN 202210422410 A CN202210422410 A CN 202210422410A CN 114672439 A CN114672439 A CN 114672439A
- Authority
- CN
- China
- Prior art keywords
- culture
- user
- strain
- setting
- culture solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000894006 Bacteria Species 0.000 title claims abstract description 39
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 title claims abstract description 29
- 230000033228 biological regulation Effects 0.000 title claims abstract description 14
- 238000012136 culture method Methods 0.000 title description 9
- 238000000034 method Methods 0.000 claims abstract description 27
- 238000005286 illumination Methods 0.000 claims abstract description 23
- 230000001580 bacterial effect Effects 0.000 claims abstract description 22
- 238000001035 drying Methods 0.000 claims abstract description 20
- 239000000463 material Substances 0.000 claims abstract description 20
- 238000012544 monitoring process Methods 0.000 claims abstract description 13
- 230000008569 process Effects 0.000 claims abstract description 12
- 238000002156 mixing Methods 0.000 claims abstract description 10
- 230000001954 sterilising effect Effects 0.000 claims abstract description 10
- 238000003756 stirring Methods 0.000 claims abstract description 10
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 claims description 10
- 238000013528 artificial neural network Methods 0.000 claims description 10
- GNFTZDOKVXKIBK-UHFFFAOYSA-N 3-(2-methoxyethoxy)benzohydrazide Chemical compound COCCOC1=CC=CC(C(=O)NN)=C1 GNFTZDOKVXKIBK-UHFFFAOYSA-N 0.000 claims description 9
- 230000000968 intestinal effect Effects 0.000 claims description 9
- 230000000007 visual effect Effects 0.000 claims description 9
- 238000012545 processing Methods 0.000 claims description 5
- 238000004364 calculation method Methods 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 230000004927 fusion Effects 0.000 claims description 3
- 230000006698 induction Effects 0.000 claims description 3
- 238000007500 overflow downdraw method Methods 0.000 claims description 3
- 238000012549 training Methods 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 10
- 230000008901 benefit Effects 0.000 abstract description 4
- 238000012364 cultivation method Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012806 monitoring device Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a multi-base number regulation and control method based on sulfide bacteria, which comprises the following steps: step a: sterilizing instruments required by the culture by a user, and drying the sterilized instruments by a drying device; step b: selecting a culture solution material by a user, pouring the culture solution material into a mixer by the user, and efficiently stirring and mixing the culture solution by the mixer; step c: the bacterial culture solution is poured into the culture dish by a user, the bacterial strain is added into the culture dish by the user, and the culture dish is moved to the incubator by the user. The invention realizes the purpose of good use effect, and has the advantages of high-precision temperature control, humidity control, illumination control and monitoring, thereby avoiding the failure or low activity of bacterial colony cultivation, improving the experience of users on the sulfide bacteria cultivation process, meeting the requirements of the current market, and solving the problem of poor use effect of the conventional sulfide bacteria cultivation process.
Description
Technical Field
The invention relates to the technical field of sulfide bacteria cultivation, in particular to a multi-base number regulation and control sulfide bacteria-based cultivation method.
Background
The sulphide bacteria belongs to aerobic chemoautotrophic bacteria, the growth and reduction characteristics of the sulphide bacteria mainly determine the quantity of matrix (reducing sulphide) required by the bacteria in soil and the suitable degree of change of micro-domain environmental conditions around cells, the sulphide bacteria cultivation process is used for cultivating the sulphide bacteria, but most of the conventional sulphide bacteria cultivation processes have poor using effect and do not have the advantages of high-precision temperature control, humidity control, illumination control and monitoring, so that colony cultivation failure or low activity is caused, the experience of a user on the sulphide bacteria cultivation process is reduced, the requirements of the current market cannot be met, and due to the problems, a multi-base regulation and control sulphide bacteria-based cultivation method is pertinently provided.
Disclosure of Invention
The invention aims to provide a multi-base numerical control and sulfide bacteria-based culture method, which has the advantage of good use effect and solves the problem of poor use effect of the conventional sulfide bacteria culture process.
In order to achieve the purpose, the invention provides the following technical scheme: a multi-base regulation and sulfide bacteria-based culture method comprises the following steps:
step a: sterilizing instruments required by the culture by a user, and drying the sterilized instruments by a drying device;
step b: selecting a culture solution material by a user, pouring the culture solution material into a mixer by the user, and efficiently stirring and mixing the culture solution by the mixer;
step c: pouring the bacterial culture solution into a culture dish by a user, adding the bacterial strain into the culture dish by the user, and moving the culture dish to an incubator by the user;
step d: setting parameters of the incubator by a user, setting a temperature value, a humidity value and an illumination value of the culture, and replacing the culture solution in the culture dish by taking time as a unit;
step e: the strain sample in the culture dish is taken out at regular time by a user, the user observes the strain sample through a microscope to know the growth condition of the strain, the intestinal bacteria culture is observed in real time through monitoring equipment, and observation data are recorded and stored;
step f: when the strain culture observation is about to enter a key time period, sending a prompt, and printing the observation data of the time period in real time when the key time period is over;
step g: when the strain is qualified, the strain can be put into use.
Preferably, the culture temperature in step d is set, the culture temperature in different time periods is set according to a strain culture temperature curve, and the rise and fall time between the temperatures in different time periods is set according to the requirement of strain culture.
Preferably, the culture humidity in step d is set, the culture humidity in different time periods is set according to a strain culture humidity curve, and the rise and fall time between the humidities in different time periods is set according to the requirement of strain culture.
Preferably, the culture illumination in step d is set, the culture illumination in different time periods is set according to a strain culture illumination curve, and the intensity time between the illumination in different time periods is set according to the requirement of strain culture.
Preferably, the incubator in the step C is controlled by a controller, the controller adopts an AT89C51 single chip microcomputer, an AT89C51 single chip microcomputer adopts a BP neural network algorithm, a neural network is used as a data fusion method and is applied to various fields, the neural network has induction, summarization, extraction, memory, association and fault tolerance, the aim of processing information is achieved by adjusting the interconnection relationship among a large number of internal nodes according to the complexity of the system, in the application process, relevant knowledge and information are obtained through training, the actually measured data are compared with the information, and data fusion and edge calculation are performed.
Preferably, the prompt in the step f is realized through an alarm device, the alarm device is an audible and visual alarm, and the controller activates the audible and visual alarm to work for audible and visual alarm in the first 30 seconds to 60 seconds before the set key time period is reached.
Preferably, the monitoring device in step e adopts an infrared camera, and the infrared camera includes a lens, a photosensitive element and a signal processing element.
Compared with the prior art, the invention has the following beneficial effects:
the invention realizes the purpose of good use effect, and has the advantages of high-precision temperature control, humidity control, illumination control and monitoring, thereby avoiding the failure or low activity of bacterial colony cultivation, improving the experience of users on the sulfide bacteria cultivation process, meeting the requirements of the current market and solving the problem of poor use effect of the conventional sulfide bacteria cultivation process.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
A multi-base regulation and sulfide bacteria-based culture method comprises the following steps:
step a: sterilizing instruments required by the culture by a user, and drying the sterilized instruments by a drying device;
step b: selecting a culture solution material by a user, pouring the culture solution material into a mixer by the user, and efficiently stirring and mixing the culture solution by the mixer;
step c: pouring the bacterial culture solution into a culture dish by a user, adding the bacterial strain into the culture dish by the user, and moving the culture dish to an incubator by the user;
step d: setting parameters of the incubator by a user, setting a temperature value, a humidity value and an illumination value of the culture, and replacing the culture solution in the culture dish by taking time as a unit;
step e: the strain sample in the culture dish is taken out at regular time by a user, the user observes the strain sample through a microscope to know the growth condition of the strain, the intestinal bacteria culture is observed in real time through monitoring equipment, and observation data are recorded and stored;
step f: when the strain culture observation is about to enter a key time period, sending a prompt, and printing the observation data of the time period in real time when the key time period is over;
step g: when the strain is qualified, the strain can be put into use.
Example 1
A multi-base regulation and sulfide bacteria-based culture method comprises the following steps:
step a: sterilizing instruments required by the culture by a user, and drying the sterilized instruments by a drying device;
step b: selecting a culture solution material by a user, pouring the culture solution material into a mixer by the user, and efficiently stirring and mixing the culture solution by the mixer;
step c: pouring the bacterial culture solution into a culture dish by a user, adding the bacterial strain into the culture dish by the user, and moving the culture dish to an incubator by the user;
step d: setting parameters of the incubator by a user, setting a temperature value, a humidity value and an illumination value of the culture, and replacing the culture solution in the culture dish by taking time as a unit;
step e: the strain sample in the culture dish is taken out at regular time by a user, the user observes the strain sample through a microscope to know the growth condition of the strain, the intestinal bacteria culture is observed in real time through monitoring equipment, and observation data are recorded and stored;
step f: when the strain culture observation is about to enter a key time period, sending a prompt, and printing the observation data of the time period in real time when the key time period is over;
step g: when the strain is qualified, the strain can be put into use.
Example 2
In example 1, the following additional steps were added:
and d, setting the culture temperature in the step d, setting the culture temperature in different time periods according to a strain culture temperature curve, and setting the lifting time between the temperatures in different time periods according to the requirements of strain culture.
A multi-base regulation and sulfide bacteria-based culture method comprises the following steps:
a, step a: sterilizing instruments required by the culture by a user, and drying the sterilized instruments by a drying device;
step b: selecting a culture solution material by a user, pouring the culture solution material into a mixer by the user, and efficiently stirring and mixing the culture solution by the mixer;
step c: pouring the bacterial culture solution into a culture dish by a user, adding the bacterial strain into the culture dish by the user, and moving the culture dish to an incubator by the user;
step d: setting parameters of the incubator by a user, setting a temperature value, a humidity value and an illumination value of the culture, and replacing the culture solution in the culture dish by taking time as a unit;
step e: the strain sample in the culture dish is taken out at regular time by a user, the user observes the strain sample through a microscope to know the growth condition of the strain, the intestinal bacteria culture is observed in real time through monitoring equipment, and observation data are recorded and stored;
step f: when the strain culture observation is about to enter a key time period, sending a prompt, and printing the observation data of the time period in real time when the key time period is over;
step g: when the strain is qualified, the strain can be put into use.
Example 3
In example 2, the following steps were added:
and d, setting culture humidity, namely setting the culture humidity in different time periods according to a strain culture humidity curve, and setting the lifting time between the humidities in different time periods according to the strain culture requirement.
A multi-base regulation and sulfide bacteria-based culture method comprises the following steps:
step a: sterilizing instruments required by the culture by a user, and drying the sterilized instruments by a drying device;
step b: selecting a culture solution material by a user, pouring the culture solution material into a mixer by the user, and efficiently stirring and mixing the culture solution by the mixer;
step c: pouring the bacterial culture solution into a culture dish by a user, adding the bacterial strain into the culture dish by the user, and moving the culture dish to an incubator by the user;
step d: setting parameters of the incubator by a user, setting a temperature value, a humidity value and an illumination value of the culture, and replacing the culture solution in the culture dish by taking time as a unit;
step e: the strain sample in the culture dish is taken out at regular time by a user, the user observes the strain sample through a microscope to know the growth condition of the strain, the intestinal bacteria culture is observed in real time through monitoring equipment, and observation data are recorded and stored;
step f: when the strain culture observation is about to enter a key time period, sending a prompt, and printing the observation data of the time period in real time when the key time period is over;
step g: when the strain is qualified, the strain can be put into use.
Example 4
In example 3, the following steps were added:
and d, setting culture illumination, namely setting the culture illumination in different time periods according to a strain culture illumination curve, and setting the intensity time between the illumination in different time periods according to the requirements of strain culture.
A multi-base regulation and sulfide bacteria-based culture method comprises the following steps:
step a: sterilizing instruments required by the culture by a user, and drying the sterilized instruments by a drying device;
step b: selecting a culture solution material by a user, pouring the culture solution material into a mixer by the user, and efficiently stirring and mixing the culture solution by the mixer;
step c: pouring the bacterial culture solution into a culture dish by a user, adding the bacterial strain into the culture dish by the user, and moving the culture dish to an incubator by the user;
step d: setting parameters of the incubator by a user, setting a temperature value, a humidity value and an illumination value of the culture, and replacing the culture solution in the culture dish by taking time as a unit;
step e: the strain sample in the culture dish is taken out at regular time by a user, the user observes the strain sample through a microscope to know the growth condition of the strain, the intestinal bacteria culture is observed in real time through monitoring equipment, and observation data are recorded and stored;
step f: when the strain culture observation is about to enter a key time period, sending a prompt, and printing the observation data of the time period in real time when the key time period is over;
step g: when the strain is qualified, the strain can be put into use.
Example 5
In example 4, the following steps were added:
the incubator is controlled by a controller in the step C, the controller adopts an AT89C51 singlechip, the AT89C51 singlechip adopts a BP neural network algorithm, the neural network is used as a data fusion method and is applied to various fields, the neural network has the functions of induction, summarization, extraction, memory, association and fault tolerance, the aim of processing information is fulfilled by adjusting the interconnection relationship among a large number of internal nodes according to the complexity of the system, in the application process, relevant knowledge and information are obtained through training, the actually measured data is compared with the information, and data fusion and edge calculation are carried out.
A multi-base regulation and sulfide bacteria-based culture method comprises the following steps:
step a: sterilizing instruments required by the culture by a user, and drying the sterilized instruments by a drying device;
step b: selecting a culture solution material by a user, pouring the culture solution material into a mixer by the user, and efficiently stirring and mixing the culture solution by the mixer;
step c: pouring the bacterial culture solution into a culture dish by a user, adding the bacterial strain into the culture dish by the user, and moving the culture dish to an incubator by the user;
step d: setting parameters of the incubator by a user, setting a temperature value, a humidity value and an illumination value of the culture, and replacing the culture solution in the culture dish by taking time as a unit;
step e: the strain sample in the culture dish is taken out at regular time by a user, the user observes the strain sample through a microscope to know the growth condition of the strain, the intestinal bacteria culture is observed in real time through monitoring equipment, and observation data are recorded and stored;
step f: when the strain culture observation is about to enter a key time period, sending a prompt, and printing the observation data of the time period in real time when the key time period is over;
step g: when the strain is qualified, the strain can be put into use.
Example 6
In example 5, the following steps were added:
and f, prompting is realized through an alarm device, the alarm device is an audible and visual alarm, and the controller activates the audible and visual alarm to work for audible and visual alarm in the first 30 seconds to 60 seconds before the set key time interval is reached.
A multi-base regulation and control method for culturing sulfide-based bacteria comprises the following steps:
step a: sterilizing instruments required by the culture by a user, and drying the sterilized instruments by a drying device;
step b: selecting a culture solution material by a user, pouring the culture solution material into a mixer by the user, and efficiently stirring and mixing the culture solution by the mixer;
step c: pouring the bacterial culture solution into a culture dish by a user, adding the bacterial strain into the culture dish by the user, and moving the culture dish to an incubator by the user;
step d: setting parameters of the incubator by a user, setting a temperature value, a humidity value and an illumination value of the culture, and replacing the culture solution in the culture dish by taking time as a unit;
step e: the strain sample in the culture dish is taken out at regular time by a user, the user observes the strain sample through a microscope to know the growth condition of the strain, the intestinal bacteria culture is observed in real time through monitoring equipment, and observation data are recorded and stored;
step f: when the strain culture observation is about to enter a key time period, sending a prompt, and printing the observation data of the time period in real time when the key time period is over;
step g: when the strain is qualified, the strain can be put into use.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. A multi-base regulation and control method based on sulfide bacteria is characterized in that: the method comprises the following steps:
a, step a: sterilizing instruments required by the culture by a user, and drying the sterilized instruments by a drying device;
step b: selecting a culture solution material by a user, pouring the culture solution material into a mixer by the user, and efficiently stirring and mixing the culture solution by the mixer;
step c: pouring the bacterial culture solution into a culture dish by a user, adding the bacterial strain into the culture dish by the user, and moving the culture dish to an incubator by the user;
step d: setting parameters of the incubator by a user, setting a temperature value, a humidity value and an illumination value of the culture, and replacing the culture solution in the culture dish by taking time as a unit;
step e: the strain sample in the culture dish is taken out at regular time by a user, the user observes the strain sample through a microscope to know the growth condition of the strain, the intestinal bacteria culture is observed in real time through monitoring equipment, and observation data are recorded and stored;
step f: when the strain culture observation is about to enter a key time period, sending a prompt, and printing the observation data of the time period in real time when the key time period is over;
step g: when the strain is qualified, the strain can be put into use.
2. The method of claim 1, wherein the method comprises: and d, setting the culture temperature in the step d, setting the culture temperature in different time periods according to a strain culture temperature curve, and setting the lifting time between the temperatures in different time periods according to the requirements of strain culture.
3. The method of claim 1, wherein the method comprises: and d, setting the culture humidity in the step d, setting the culture humidity in different time periods according to a strain culture humidity curve, and setting the lifting time between the humidities in different time periods according to the strain culture requirement.
4. A multi-base regulated sulfide bacteria-based cultivation process according to claim 1, wherein: and d, setting culture illumination in the step d, setting culture illumination in different time periods according to a strain culture illumination curve, and setting the intensity time between different time periods according to the requirements of strain culture.
5. The method of claim 1, wherein the method comprises: the incubator in the step C is controlled by a controller, the controller adopts an AT89C51 singlechip, the AT89C51 singlechip adopts a BP neural network algorithm, the neural network is used as a data fusion method and is applied to various fields, the neural network has induction, summarization, extraction, memory, association and fault tolerance, the aim of processing information is fulfilled by adjusting the interconnection relationship among a large number of nodes inside the neural network according to the complexity of the system, and in the application process, related knowledge and information are obtained through training, the actually measured data is compared with the information, and data fusion and edge calculation are carried out.
6. The method of claim 1, wherein the method comprises: and f, prompting is realized through an alarm device, the alarm device is an audible and visual alarm, and the controller activates the audible and visual alarm to work for audible and visual alarm in the first 30 seconds to 60 seconds before the set key time interval is reached.
7. The method of claim 1, wherein the method comprises: and e, adopting an infrared camera by the monitoring equipment, wherein the infrared camera comprises a lens, a photosensitive element and a signal processing element.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210422410.XA CN114672439A (en) | 2022-04-21 | 2022-04-21 | Multi-base-number regulation and control culture method based on sulfide bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210422410.XA CN114672439A (en) | 2022-04-21 | 2022-04-21 | Multi-base-number regulation and control culture method based on sulfide bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114672439A true CN114672439A (en) | 2022-06-28 |
Family
ID=82079427
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210422410.XA Pending CN114672439A (en) | 2022-04-21 | 2022-04-21 | Multi-base-number regulation and control culture method based on sulfide bacteria |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114672439A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100239555A1 (en) * | 2007-07-18 | 2010-09-23 | Rakesh Govind | Enzymatically active compositions for suppressing sulfide generation and methods of use thereof |
| CN106635866A (en) * | 2016-09-08 | 2017-05-10 | 南京师范大学 | Thiobacillus thioparus and high-activity culture method |
| CN109628355A (en) * | 2019-01-31 | 2019-04-16 | 浙江卫星能源有限公司 | A kind of sulfide degradation bacteria and its application |
| CN112725237A (en) * | 2021-01-27 | 2021-04-30 | 川北医学院附属医院 | Intestinal bacteria culture observation method |
| CN112746032A (en) * | 2019-10-30 | 2021-05-04 | 中国石油化工股份有限公司 | Enrichment culture method of sulfur bacteria |
-
2022
- 2022-04-21 CN CN202210422410.XA patent/CN114672439A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100239555A1 (en) * | 2007-07-18 | 2010-09-23 | Rakesh Govind | Enzymatically active compositions for suppressing sulfide generation and methods of use thereof |
| CN106635866A (en) * | 2016-09-08 | 2017-05-10 | 南京师范大学 | Thiobacillus thioparus and high-activity culture method |
| CN109628355A (en) * | 2019-01-31 | 2019-04-16 | 浙江卫星能源有限公司 | A kind of sulfide degradation bacteria and its application |
| CN112746032A (en) * | 2019-10-30 | 2021-05-04 | 中国石油化工股份有限公司 | Enrichment culture method of sulfur bacteria |
| CN112725237A (en) * | 2021-01-27 | 2021-04-30 | 川北医学院附属医院 | Intestinal bacteria culture observation method |
Non-Patent Citations (1)
| Title |
|---|
| 杨颐康等: "硫化细菌的分离培养", 微生物学报, vol. 10, no. 4, pages 521 - 522 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102647475B (en) | A kind of intelligentized Internet of things system of livestock and poultry farm and Internet of Things method | |
| KR101136015B1 (en) | Remote Control System of Microbial incubator | |
| CN109634098A (en) | A kind of fattening house environment conditioning system and method | |
| CN104396850A (en) | Industrial aquaculture system and intelligent control system thereof | |
| CN106850831A (en) | A kind of automatic intelligent cultivating system | |
| CN105638507A (en) | System achieving automatic control and circulating utilization of plant nutrient solution | |
| CN111254229B (en) | Carbon dioxide incubator control method, control system and carbon dioxide incubator | |
| CN109111030A (en) | Integrated sewage disposal intelligence control system and control method | |
| JP2002055708A (en) | Fermentation monitoring device | |
| CN110476901A (en) | A method of extending Candle-sticks stinkbug ovum shelf life | |
| CN102084844B (en) | Automatic alkali adding method for culture pool | |
| CN114672439A (en) | Multi-base-number regulation and control culture method based on sulfide bacteria | |
| CN106718692A (en) | One kind automation agricultural irrigation systems | |
| CN204174215U (en) | A kind of thermostat container | |
| CN101423808A (en) | Resuscitation fluids of VBNC salmonella and preparation method thereof and resuscitation method | |
| CN207452120U (en) | A kind of microbiological incubator | |
| CN116520740A (en) | Aquaculture monitored control system based on AI discernment | |
| CN105785951B (en) | The processing automatic operating system of sewage is realized using statistical modeling technology | |
| CN209268397U (en) | Control system for fish multiplication pouring station circulating water treatment device | |
| CN208091497U (en) | A kind of putrid and deteriorated monitoring device of fruits and vegetables | |
| CN103792962A (en) | Dissolved oxygen and temperature monitoring and gas intelligent controller | |
| CN110132691A (en) | A kind of wild siberian wildrye chromosome flaking method in High-cold regions | |
| CN113983659B (en) | Fresh air system control method and control device thereof | |
| CN109362638A (en) | Control system for fish multiplication pouring station circulating water treatment device | |
| CN211020264U (en) | Full-automatic plant breeding monitoring system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220628 |