CN114671721A - Method for preparing brown algae extract, extracting solution and application - Google Patents
Method for preparing brown algae extract, extracting solution and application Download PDFInfo
- Publication number
- CN114671721A CN114671721A CN202210196356.1A CN202210196356A CN114671721A CN 114671721 A CN114671721 A CN 114671721A CN 202210196356 A CN202210196356 A CN 202210196356A CN 114671721 A CN114671721 A CN 114671721A
- Authority
- CN
- China
- Prior art keywords
- brown algae
- algae extract
- reaction system
- enzymolysis
- thousand
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 32
- 239000000284 extract Substances 0.000 title claims abstract description 29
- 241000199919 Phaeophyceae Species 0.000 title claims abstract description 22
- 241000208822 Lactuca Species 0.000 claims description 24
- 235000003228 Lactuca sativa Nutrition 0.000 claims description 24
- 102000004190 Enzymes Human genes 0.000 claims description 22
- 108090000790 Enzymes Proteins 0.000 claims description 22
- 229940088598 enzyme Drugs 0.000 claims description 22
- 241001474374 Blennius Species 0.000 claims description 21
- 230000000694 effects Effects 0.000 claims description 19
- 239000000243 solution Substances 0.000 claims description 18
- 238000011282 treatment Methods 0.000 claims description 17
- 238000006243 chemical reaction Methods 0.000 claims description 14
- 108010004131 poly(beta-D-mannuronate) lyase Proteins 0.000 claims description 12
- 108010059892 Cellulase Proteins 0.000 claims description 11
- 229940106157 cellulase Drugs 0.000 claims description 11
- 241000195474 Sargassum Species 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 9
- 238000009832 plasma treatment Methods 0.000 claims description 9
- 238000004321 preservation Methods 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 9
- 238000010298 pulverizing process Methods 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 238000010790 dilution Methods 0.000 claims description 4
- 239000012895 dilution Substances 0.000 claims description 4
- 238000005507 spraying Methods 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 2
- 239000002994 raw material Substances 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims description 2
- 239000003337 fertilizer Substances 0.000 abstract description 4
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 12
- 241000512259 Ascophyllum nodosum Species 0.000 description 11
- 238000005516 engineering process Methods 0.000 description 10
- 229910002651 NO3 Inorganic materials 0.000 description 7
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 7
- 239000008399 tap water Substances 0.000 description 7
- 235000020679 tap water Nutrition 0.000 description 7
- 238000002156 mixing Methods 0.000 description 6
- 230000035882 stress Effects 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 4
- 230000001105 regulatory effect Effects 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 230000008961 swelling Effects 0.000 description 4
- 102000019197 Superoxide Dismutase Human genes 0.000 description 3
- 108010012715 Superoxide dismutase Proteins 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000004480 active ingredient Substances 0.000 description 2
- 230000008645 cold stress Effects 0.000 description 2
- 230000000593 degrading effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000000053 physical method Methods 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 108010059820 Polygalacturonase Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000005422 blasting Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 108010093305 exopolygalacturonase Proteins 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000000383 hazardous chemical Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 239000003501 hydroponics Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000002786 root growth Effects 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/15—Leaf crops, e.g. lettuce or spinach
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Environmental Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Botany (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Fertilizers (AREA)
Abstract
The invention relates to a method for preparing a brown algae extract, an extracting solution and application, and relates to the field of agricultural fertilizers.
Description
Technical Field
The invention relates to the field of agricultural fertilizers, and particularly discloses a method for preparing a brown algae extract, an extracting solution and application.
Background
The brown algae extract is widely applied to the industries of fertilizers, feeds and the like at home and abroad, and the production and preparation technology mainly adopts a chemical method, a physical method, a biological enzyme method and the like. The chemical method has a certain destructive effect on active ingredients in the seaweed, has a lower extraction rate than other technologies such as a biological enzyme method and the like, and has lower cost. The physical method mainly comprises mechanical crushing and low-temperature blasting, the method can well reserve natural active ingredients in the seaweed, is environment-friendly and pollution-free, but the equipment investment is high, and only a few companies such as Kelpak in south Africa adopt the method. The biological enzymolysis technology is a hotspot in the industry in recent years, and patents and articles disclose that brown algae can be effectively degraded by using alginate lyase, cellulase, protease, pectinase and the like to obtain a brown algae extract, but the current commercial alginate lyase or brown algae degrading enzyme is rare and expensive, so that the cost for preparing the brown algae extract by using the biological enzyme method is difficult to be accepted by practical production.
The plasma technology is a novel technology, generates a large amount of active hydrogen through plasma discharge, can endow various excellent properties to the surface of a material by contacting the surface of the material without changing the properties of a base material, and is widely applied to various material surface processing treatment processes, such as automobile manufacturing, digital printing, semiconductor technology, medicines and cosmetics, textiles and the like. Plasma technology allows the manufacturing process to be more efficient and environmentally friendly by eliminating many expensive, complex and environmentally hazardous chemical treatment steps. In recent years, the application range of the plasma technology is further expanded, the plasma technology can promote the cracking and state change of polymer molecules and further endow the polymer molecules with new biological activity, such as the characteristics of oxidation resistance, antibiosis and the like, the method is simple, environment-friendly and pollution-free, and the method is a technical method suitable for large-scale popularization.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a method for preparing a brown algae extract, an extracting solution and application, wherein the method is characterized in that the use amount of biological enzymes extracted from brown algae is reduced by combining a plasma technology and biological enzymolysis, the enzymolysis cost is reduced, the production and popularization are facilitated, the traditional strong acid and strong base chemical method is thoroughly replaced, the obtained brown algae extracting solution is directly applied to the cultivation process of water-cultured lettuce, the quality of the water-cultured lettuce is effectively improved, and the influence of low-temperature stress on the lettuce can be effectively relieved.
The technical scheme adopted by the invention is as follows:
a method for preparing brown algae extract comprises the following steps:
(1) pretreatment of raw materials: pulverizing dried Sargassum into 40-80 mesh, adding 6-12 times of water;
(2) low-temperature plasma treatment: using a direct current pulse power supply with the output voltage of 0-2KV, the duty ratio of 5-50% and the frequency of 50-300Hz pulse wave; placing the reaction system below an electrode, wherein the distance between the electrode and the liquid level of the reaction system is 10-30mm, the distance between the electrode and the liquid level of the reaction system is 2-10mm, and the treatment time is 5-30 minutes;
(3) and (3) enzymolysis treatment: adjusting the pH value of the reaction system treated in the step (2) to 6.0-7.5, keeping the temperature at 40-45 ℃, adding 0.5-1% (mass ratio) of cellulase with the enzyme activity of 10-20 ten thousand U/g and 0.01-0.05% (mass ratio) of alginate lyase with the enzyme activity of 20-30 ten thousand U/g, and carrying out enzymolysis for 3-5h under the conditions of heat preservation and stirring;
(4) separating to obtain a seaweed extract: performing solid-liquid separation by centrifuging or filtering to obtain Sargassum extractive solution with solid content of 7-12%;
the invention also provides the brown algae extract obtained by the method.
The invention also provides an application of the brown algae extract, and the application method comprises the following steps:
the brown algae extract diluted by 200-fold and 1000-fold is used for lettuce cultivation by spraying on leaf surfaces, the root growth of lettuce can be remarkably promoted, the quality of lettuce is improved, the nitrate content is reduced, and meanwhile, the influence of low-temperature stress on the growth and development of lettuce can be effectively relieved.
Compared with the prior art, the invention has the following beneficial effects:
the method effectively reduces the enzyme dosage and enzymolysis time for degrading the brown algae by the aid of plasma treatment, thereby obtaining the brown algae extracting solution with high extraction rate. Further, the fertilizer is applied to lettuce culture by foliage spraying, so that the quality and the stress resistance of the lettuce can be effectively improved, the nitrate content in the lettuce is obviously reduced, and the effect of resisting low-temperature stress is obvious.
Drawings
FIG. 1 variation of nitrate content in lettuce in different treatment groups.
FIG. 2 change of superoxide dismutase content after low temperature stress of lettuce in different treatment groups
Detailed Description
The technical solution of the present invention is further explained below with reference to the embodiments and the drawings, but the scope of the present invention is not limited in any way by the embodiments.
In the examples, cellulase is from Hirshi (Beijing) Biotechnology development, Inc., and alginate lyase is independently developed in the laboratory (see patent No. 202010170519. X).
Example 1
(1) Pulverizing dried herba Zosterae Marinae into about 10g of 50 mesh, adding tap water 8 times of the dried herba Zosterae Marinae, and mixing;
(2) low-temperature plasma treatment: and (3) placing the electrodes above the reaction system by using direct current pulse voltage with the output voltage of 2KV, the duty ratio of 30% and the frequency of 200Hz, wherein the distance between the 2 electrodes is 20mm, the distance between the electrodes and the liquid level of the reaction system is 2mm, and treating for 30 min.
(3) And (3) enzymolysis treatment: regulating the pH value of the system to 7.0 by using potassium hydroxide, adding 0.7% of cellulase with the enzyme activity of 20 ten thousand U/g and 0.01% of alginate lyase with the enzyme activity of 30 ten thousand U/g at the temperature of 40 ℃, and carrying out enzymolysis for 3 hours under the conditions of heat preservation and stirring.
(4) Centrifuging to obtain a seaweed extract: centrifuging at 4000rpm for 5min by a centrifuge, removing non-enzymolysis Sargassum residue, and obtaining supernatant (Sargassum extract) with solid content of about 10%.
In addition: simple enzymolysis under the same conditions as above: adding 8 times of tap water by weight into 50 meshes of dried kelp, uniformly mixing, preheating at 40 ℃ and swelling for 30min to replace a low-temperature plasma treatment process, adding 0.7% of cellulase with 20 ten thousand U/g of enzyme activity and 0.01% of alginate lyase with 30 ten thousand U/g of enzyme activity at 40 ℃ with pH of 7.0, carrying out enzymolysis for 3h under the condition of heat preservation and stirring, and centrifuging at 4000rpm for 5min to separate and prepare kelp extract.
In experiments, the fact that only plasma is used for treating high-viscosity seaweed paste can only partially reduce the viscosity of a system within a short time (30min-60min) and cannot completely degrade the seaweed is found, the seaweed component is excessively oxidized and turns black after being treated for 1-2h or more for a long time, and the characteristics and activity of the seaweed are lost.
Example 2
(1) Pulverizing dried herba Zosterae Marinae into about 10g of 80 mesh, adding 10 times of tap water, and mixing;
(2) Low-temperature plasma treatment: and (3) placing the electrodes above the reaction system by using direct current pulse voltage with the output voltage of 1KV, the duty ratio of 20% and the frequency of 300Hz, wherein the distance between 2 electrodes is 15mm, the distance between the electrodes and the liquid level of the reaction system is 3mm, and treating for 20 min.
(3) And (3) enzymolysis treatment: regulating the pH value of the system to 6.5 by using potassium hydroxide, adding 0.5 percent of cellulase with 20 ten thousand U/g of enzyme activity and 0.02 percent of alginate lyase with 30 ten thousand U/g of enzyme activity at the temperature of 45 ℃, and carrying out enzymolysis for 4 hours under the conditions of heat preservation and stirring.
(4) Centrifuging to obtain a seaweed extract: centrifuging at 5000rpm for 3min, removing residue of non-enzymolysis Sargassum to obtain supernatant (Sargassum extractive solution) with solid content of about 8%.
In addition: simple enzymolysis under the same conditions as above: adding 10 times of tap water by weight into the dried kelp of 80 meshes, uniformly mixing, preheating at 45 ℃ and swelling for 30min to replace the low-temperature plasma treatment process, adding 0.5% of cellulase with 20 ten thousand U/g of enzyme activity and 0.02% of alginate lyase with 30 ten thousand U/g of enzyme activity at 45 ℃ of pH6.5, carrying out enzymolysis for 4h under the condition of heat preservation and stirring, and centrifuging at 5000rpm for 3min to separate and prepare kelp extract.
Example 3
(1) Pulverizing dried herba Zosterae Marinae into about 10g of 40 mesh, adding tap water 12 times of the dried herba Zosterae Marinae, and mixing;
(2) Low-temperature plasma treatment: and (3) placing the electrodes above the reaction system by using direct current pulse voltage with the output voltage of 1.5KV, the duty ratio of 15% and the frequency of 150Hz, wherein the distance between 2 electrodes is 10mm, the distance between the electrodes and the liquid level of the reaction system is 1mm, and treating for 25 min.
(3) And (3) enzymolysis treatment: regulating the pH value of the system to 7.5 by using potassium hydroxide, regulating the temperature to 42 ℃, adding 0.7 percent of cellulase with the enzyme activity of 10 ten thousand U/g and 0.02 percent of alginate lyase with the enzyme activity of 20 ten thousand U/g, and carrying out enzymolysis for 4 hours under the conditions of heat preservation and stirring.
(4) Centrifuging to obtain a seaweed extract: centrifuging at 4000rpm for 3min, removing non-enzymolysis Sargassum residue, and obtaining supernatant with solid content of about 7%, i.e. Sargassum extractive solution.
Simple enzymolysis under the same conditions as above: adding tap water with the weight being 12 times of that of the dried kelp with the mesh of 40 meshes, uniformly mixing, preheating at 42 ℃ and swelling for 25min to replace the low-temperature plasma treatment process, adding 0.7% of cellulase with the enzyme activity of 10 million U/g and 0.02% of alginate lyase with the enzyme activity of 20 million U/g at the temperature of 42 ℃ under the conditions of pH7.5, carrying out enzymolysis for 4h under the condition of heat preservation and stirring, and centrifuging at 4000rpm for 3min to separate and prepare kelp extract.
Example 4
Preparing a kelp extracting solution by simple enzymolysis: adding 10g of dried kelp which is crushed into 50 meshes into tap water with the weight 10 times that of the kelp, adjusting the pH value to be 7.0 by KOH, preheating and swelling for 2h at 45 ℃, adding 2.0 percent of 20 ten thousand U/g of cellulase and 0.1 percent of 30 ten thousand U/g of alginate lyase, performing enzymolysis for 10h under the condition of heat preservation and stirring, and centrifuging for 3min at 5000rpm to obtain kelp extract.
Example 5 application of seaweed extract to hydroponics of lettuce
The seaweed extracts of examples 3 and 4 were diluted 300 times, 500 times, 800 times, respectively, and applied to hydroponic lettuce by foliar spray. The application is carried out 1 time on the planting day, and 1 time is carried out every 10 days after the planting, the application is carried out 4 times in total, and the spraying amount is preferably that the foliage drops. And (4) taking clear water as a control group, and detecting the nitrate content and the shelf life after all treatments are cultured to a harvesting period (planting for 35 days).
After 2 weeks of field planting of the lettuce applied with the seaweed extract, artificially cooling to 5 ℃ and keeping for 12 hours, then recovering to 18 ℃ for normal cultivation and detecting the content of superoxide dismutase in the lettuce.
The detection result of the nitrate content of the harvested lettuce shows that the nitrate content of the lettuce can be effectively reduced by applying the seaweed extracting solution, the effect of each dilution degree of the plasma combined enzymolysis treatment group is better than that of the single enzymolysis group, the nitrate content of the lettuce in the seaweed extracting solution application group diluted by 500 times in the combined treatment group is reduced by about 41.6 percent compared with that of the control group, and the edible safety of the lettuce is effectively improved.
The detection result of the lettuce subjected to cold stress on superoxide dismutase SOD shows that the SOD content of the lettuce applied with the seaweed extracting solution is obviously higher than that of a control group, the SOD content of the lettuce of the seaweed extracting solution applied with the plasma combined enzymolysis treatment process is higher than that of the corresponding seaweed extracting solution subjected to simple enzymolysis, the best SOD promoting effect is obtained when the dilution degree of the seaweed extracting solutions of the two processes is 500 times, the SOD improvement can be still obviously promoted when the dilution degree of the seaweed extracting solution of the plasma combined enzymolysis process is 800 times higher, the cold stress is effectively relieved, and the stress resistance of plants is enhanced.
TABLE 1 comparison of plasma-combined enzymolysis treatment and simple enzymolysis treatment systems for kelp
Note: in examples 1, 2 and 3, the supernatant and the residue could not be separated smoothly due to the excessive viscosity of the single enzymatic hydrolysis system.
Claims (3)
1. A method for preparing brown algae extract is characterized by comprising the following steps:
(1) pretreatment of raw materials: pulverizing dried Sargassum into 40-80 mesh, adding 6-12 times of water;
(2) low-temperature plasma treatment: using a direct current pulse power supply with the output voltage of 0-2KV, the duty ratio of 5-50% and the frequency of 50-300Hz pulse wave; placing the reaction system below an electrode, wherein the distance between the electrode and the liquid level of the reaction system is 10-30mm, the distance between the electrode and the liquid level of the reaction system is 2-10mm, and the treatment time is 5-30 min;
(3) and (3) enzymolysis treatment: adjusting the pH value of the reaction system treated in the step (2) to 6.0-7.5, the temperature to 40-45 ℃, adding 0.5-1% of cellulase with the enzyme activity of 10-20 ten thousand U/g and 0.01-0.05% of alginate lyase with the enzyme activity of 20-30 ten thousand U/g, and carrying out enzymolysis for 3-5h under the conditions of heat preservation and stirring;
(4) separating to obtain a seaweed extract: performing solid-liquid separation by centrifuging or filtering to obtain Sargassum extractive solution with solid content of 7-12%.
2. The brown algae extract obtained by the method of claim 1.
3. The use of the brown algae extract of claim 2, the method comprising: the brown algae extract with the dilution of 200 and 1000 times is used for lettuce culture by spraying on leaf surfaces.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210196356.1A CN114671721A (en) | 2022-05-12 | 2022-05-12 | Method for preparing brown algae extract, extracting solution and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210196356.1A CN114671721A (en) | 2022-05-12 | 2022-05-12 | Method for preparing brown algae extract, extracting solution and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114671721A true CN114671721A (en) | 2022-06-28 |
Family
ID=82072631
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210196356.1A Pending CN114671721A (en) | 2022-05-12 | 2022-05-12 | Method for preparing brown algae extract, extracting solution and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114671721A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101705182A (en) * | 2009-11-30 | 2010-05-12 | 哈尔滨工业大学 | Biological cell wall breaking system and biological effective component extracting system based on pulsed discharge plasma technology |
| JP2014043513A (en) * | 2012-08-27 | 2014-03-13 | Nagoya Univ | Method for decomposing alginic acid, and composition composed of alginic acid and/or derivative thereof |
| CN112167268A (en) * | 2019-07-02 | 2021-01-05 | 青岛苏贝尔作物营养有限公司 | Method for producing agricultural seaweed extract by physical and biological combination method and seaweed extract |
| CN114409226A (en) * | 2021-12-21 | 2022-04-29 | 中国光大绿色技术创新研究院有限公司 | Low-temperature plasma device for residual sludge wall breaking digestion |
-
2022
- 2022-05-12 CN CN202210196356.1A patent/CN114671721A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101705182A (en) * | 2009-11-30 | 2010-05-12 | 哈尔滨工业大学 | Biological cell wall breaking system and biological effective component extracting system based on pulsed discharge plasma technology |
| JP2014043513A (en) * | 2012-08-27 | 2014-03-13 | Nagoya Univ | Method for decomposing alginic acid, and composition composed of alginic acid and/or derivative thereof |
| CN112167268A (en) * | 2019-07-02 | 2021-01-05 | 青岛苏贝尔作物营养有限公司 | Method for producing agricultural seaweed extract by physical and biological combination method and seaweed extract |
| CN114409226A (en) * | 2021-12-21 | 2022-04-29 | 中国光大绿色技术创新研究院有限公司 | Low-temperature plasma device for residual sludge wall breaking digestion |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2016029646A1 (en) | Composite microorganism enzyme, method for preparing plant nutrient solution by using composite microorganism enzyme, and fertilizer synergist | |
| CN102276326B (en) | Sargassum organic liquid fertilizer and preparation method thereof | |
| CN110295209B (en) | Technological method for extracting alginate oligosaccharides by enzymatic hydrolysis of gulfweed | |
| CN113880612A (en) | Method for preparing seaweed biological organic liquid fertilizer, seaweed biological organic liquid fertilizer and application | |
| CN113321563A (en) | Seaweed organic water-soluble fertilizer | |
| CN108743466B (en) | Preparation method and application of tea bran extract | |
| CN103864954B (en) | A kind of extracting method of peanut meal polysaccharides | |
| CN113729110B (en) | High-efficiency low-cost pretreatment combined solid state fermentation method for biomass material and application of biomass material in single-cell protein feed production | |
| CN114989984B (en) | Method for preparing seaweed extract by enzymolysis | |
| CN114671721A (en) | Method for preparing brown algae extract, extracting solution and application | |
| CN111393535A (en) | Extraction method of laminarin and application of laminarin in aquatic feed | |
| CN110922501A (en) | Process for extracting sodium alginate by using ultrasonic-assisted complex enzyme | |
| CN114181009A (en) | Method for preparing bio-organic fertilizer by degrading seaweed with complex enzyme | |
| CN112827998B (en) | Application of camellia oleifera glycolysis product in daily chemical raw materials | |
| CN106947796A (en) | A kind of D trehaloses purifying technique | |
| CN114315483A (en) | Humic acid water-soluble fertilizer containing seaweed extract and preparation method thereof | |
| CN114276197A (en) | Method for producing synergistic fertilizer by using food waste | |
| CN109206475B (en) | Method for simultaneously extracting oil body and protein by single-step enzymolysis | |
| CN112941120A (en) | Method for producing microbial oil by using VFAs and lignocellulose raw materials | |
| CN110613033A (en) | High-tea-polyphenol golden flower black tea powder extraction method and high-tea-polyphenol golden flower black tea powder | |
| CN116574771B (en) | Alginic acid fermentation broth and preparation method and application thereof | |
| CN113754501B (en) | Preparation method of nutrient solution for improving quality of flowering cabbage, product and application thereof | |
| CN112159762B (en) | Fusarium oxysporum SWH-3 and method for producing diosgenin by fermentation of fusarium oxysporum SWH-3 | |
| CN111172228B (en) | Method for producing biogas by using camellia oleifera shells | |
| CN114196724A (en) | Method for preparing fucoidan by biological coupling method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220628 |