CN114671532A - Preparation method of high-activity nitrifying bacteria biomembrane for aquaculture water treatment - Google Patents

Preparation method of high-activity nitrifying bacteria biomembrane for aquaculture water treatment Download PDF

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CN114671532A
CN114671532A CN202210381316.4A CN202210381316A CN114671532A CN 114671532 A CN114671532 A CN 114671532A CN 202210381316 A CN202210381316 A CN 202210381316A CN 114671532 A CN114671532 A CN 114671532A
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concentration
nitrifying bacteria
ammonia nitrogen
preparation
activity
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CN114671532B (en
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周小琳
李子浩
鞠鸿林
张源
孙鹏成
钟宛真
刘育铭
宫筱
彭天瑞
高宏宇
毕学军
程丽华
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Qingdao University of Technology
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C02F2101/166—Nitrites
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/08—Seawater, e.g. for desalination
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2305/00—Use of specific compounds during water treatment
    • C02F2305/06—Nutrients for stimulating the growth of microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

Abstract

A preparation method of a high-activity nitrifying bacteria biomembrane for aquaculture water treatment comprises the following steps: (1) adding sea sand into the water in the aquaculture pond; (2) adding a nutrient solution; (3) aerating and controlling the concentration of dissolved oxygen; (4) adding ferric hydroxide solution; (5) the ammonia nitrogen concentration is always maintained at 2-20 mg/L, and the pH is always maintained at 7-9; (6) adding a suspension carrier according to the concentration of suspended matters except sea sand in the mixed solution; (7) adjusting the concentration of dissolved oxygen; (8) stopping aeration when more than 20% of the suspended carriers turn over along with the water flow, and taking out the sea sand; (9) starting aeration and maintaining the concentration of dissolved oxygen; (10) and when ammonia nitrogen can not be detected, the preparation of the biological membrane is finished. The invention improves the fixing speed of the nitrifying bacteria biofilm, the nitrifying bacteria biofilm has high-efficiency nitration reaction activity, and the ammonia nitrogen and nitrite nitrogen in water can be rapidly removed.

Description

Preparation method of high-activity nitrifying bacteria biomembrane for aquaculture water treatment
Technical Field
The invention relates to a preparation method of a high-activity nitrifying bacteria biofilm for treating aquaculture water, and belongs to the technical field of preparation of nitrifying bacteria biofilms.
Background
In the process of aquaculture, the excrement, residual bait and the like of aquatic organisms generate ammonia nitrogen and nitrite nitrogen, and the threat to the health of the aquatic organisms is caused. The microbiological method is an economical, feasible, environment-friendly and efficient method for removing ammonia nitrogen and nitrite nitrogen in water.
The microbiological method is to remove ammonia nitrogen and nitrite nitrogen in water by using nitrifying bacteria under the aerobic condition, and the nitrifying bacteria are used as autotrophic bacteria, have long generation period and need harsh growth environment, so that the advantages of the nitrifying bacteria and heterotrophic microorganisms cannot be obtained in competition. Therefore, when the content of ammonia nitrogen or nitrite nitrogen in the aquaculture water is high, a method of adding nitrobacteria liquid into the aquaculture water is often adopted. However, the method for adding the nitrobacteria liquid cannot avoid the loss of nitrobacteria in the water changing process, and needs to be added periodically.
In order to avoid the loss of nitrifying bacteria, the aquaculture wastewater can be treated by adopting a biofilm method, pollutants in the water are removed by utilizing a biofilm attached to and grown on the filler, and the moving bed biofilm avoids the defects of easy blockage, difficult maintenance and the like of a fixed bed biofilm method, and is suitable for the aquaculture wastewater treatment.
However, as the aquaculture wastewater belongs to slightly polluted water, the speed of enriching the nitrifying bacteria biomembrane by adopting a natural method is extremely slow, and the nitrification activity is low.
Chinese patent document CN104961229A discloses a nitrifying bacteria microbial membrane and a preparation method thereof, wherein the nitrifying bacteria microbial membrane is a high-activity nitrifying bacteria membrane loaded on the surface of a porous medium carrier, which is obtained by inoculating activated sludge on the surface of the porous medium carrier and performing enrichment culture of nitrifying bacteria by adopting a continuous circulation supply method of nitrifying bacteria enrichment culture solution. The preparation process of the nitrobacteria microbial membrane is complex, and the porous medium is used as a carrier, so that the nitrobacteria microbial membrane is not suitable for efficiently removing ammonia nitrogen in an aquaculture water body.
Chinese patent document CN101240253A discloses a method for enriching efficient nitrifying bacteria in activated sludge, which adopts an intermittent activated sludge method and carries out enrichment by gradually increasing the ammonia nitrogen concentration of a culture solution, wherein the main components of the enrichment culture solution are inorganic salts comprising trace elements Fe, Mg, Na, K and buffer solution, the initial concentration of ammonia nitrogen is 100 Mg/L-200 Mg/L, and the final concentration is 500 Mg/L-1200 Mg/L; COD is less than or equal to 200 mg/L. The method can inhibit the growth of miscellaneous bacteria such as carbonized bacteria in the sludge so as to facilitate nitrifying bacteria to become dominant flora. The method is beneficial to the enrichment of efficient nitrobacteria, but the method is based on activated sludge, cannot efficiently generate a biological membrane and cannot efficiently remove ammonia nitrogen in the aquaculture water body.
Disclosure of Invention
The invention provides a preparation method of a high-activity nitrifying bacteria biomembrane for treating aquaculture water, which is used for promoting the growth of biomembrane nitrifying bacteria, and has high preparation speed and high nitrification activity, aiming at solving the problem that pollutants such as ammonia nitrogen, nitrite nitrogen and the like in an aquaculture pond are difficult to remove and the defects of the existing nitrifying bacteria biomembrane preparation technology, and solving the problem of low ammonia nitrogen removal rate of an aquaculture water body.
The invention discloses a preparation method of a high-activity nitrifying bacteria biomembrane for aquaculture water treatment, which comprises the following steps:
(1) adding sea sand (seaside fine sand) into aquaculture pond water to obtain mixture, wherein the volume of the sea sand is not more than 1/3 of the total volume of the mixture;
(2) adding 1-2% of nutrient solution into the mixture according to the volume ratio to form a mixed solution;
the preparation process of the nutrient solution comprises the following steps: adding 300-500 mg of MgSO (MgSO) into every 1000mL of distilled water4·H2O, 20-50 mg of CaCl2·H2O, 0.2-2.0 mg of MnCl2·H2O, 0.1-0.2 mg of Na2 MoO4·H2O, 0.1-0.2 mg CoCl2·H2O, 0.1-0.2 mg of ZnSO4·H2O and 80-500 mg of K2HPO4And mixing uniformly.
(3) Aerating the mixed solution, and controlling the concentration of dissolved oxygen to be more than 6 mg/L;
(4) adding iron hydroxide solution with the addition amount of 20-40 mg Fe3+/L。
Adding a sodium hydroxide solution into a ferric trichloride solution with the mass concentration of 15% and continuously stirring, stopping adding the sodium hydroxide solution until the pH value is 7-8, and adding the sodium hydroxide solution into the mixed solution while continuously stirring.
(5) Regularly monitoring the ammonia nitrogen concentration and the pH value of the mixed solution, so that the ammonia nitrogen concentration is always maintained at 2-20 mg/L, and the pH value is always maintained at 7-9;
the ammonia nitrogen concentration is maintained at 2-20 mg/L by adding ammonium sulfate or ammonium chloride.
The pH value is maintained at 7-9 by adding sodium bicarbonate.
(6) Monitoring the concentration of suspended matters (without sea sand) except sea sand in the mixed solution every day, and adding a suspension carrier when the concentration of the suspended matters is increased by 30% or more than that of the suspended matters in the mixed solution in the previous day;
the addition density of the suspension carrier is 0.94-0.97 g/cm3；
(7) Adjusting the aeration quantity to ensure that the concentration of dissolved oxygen is 2.5-4.5 mg/L;
(8) regularly observing the state of the suspended carriers, and stopping aeration and taking out the sea sand when more than 20% of the suspended carriers in the floating state change from the floating state to the rolling state along with the water flow;
(9) starting aeration, maintaining the concentration of dissolved oxygen at 2.5-4.5 mg/L, adjusting the concentration of ammonia nitrogen to 20mg/L (adding and supplementing ammonium sulfate or ammonium chloride), and then not supplementing ammonia nitrogen;
(10) and when the ammonia nitrogen can not be detected, the preparation of the biological membrane is finished.
The invention takes the suspended carrier as the microbial carrier, improves the nitrification activity of nitrifying bacteria by loading the biomembrane with iron, is suitable for the large-range temperature condition of 5-35 ℃, is suitable for mariculture and freshwater culture, greatly improves the fixing speed of the nitrifying bacteria biomembrane, has high-efficiency nitrification activity and can quickly remove ammonia nitrogen and nitrite nitrogen in water. Has the following characteristics:
1. microorganisms attached to the sea side fine sand are taken as strains, so that the method has the characteristics of low temperature resistance and salt tolerance, can be suitable for the large-range temperature condition of 5-35 ℃, and is suitable for mariculture and freshwater culture;
2. the addition of iron ions is proved to promote the growth of nitrifying bacteria and improve the nitrification activity of the nitrifying bacteria, but aiming at different processes, the addition amount and the addition mode of the iron ions are different, the invention provides the addition amount and the addition mode which are applicable to the treatment of aquaculture wastewater and are fixed on a suspension carrier, and the addition amount and the addition mode of the iron ions can promote the growth of the nitrifying bacteria of a biological membrane and improve the nitrification activity;
3. the pond water is used as a main culture medium, and the nitrifying bacteria biofilm suitable for the water body can be attached and enriched, and is suitable for mariculture and freshwater culture. Meanwhile, according to the characteristics of pond water, the invention supplements the trace element nutrient solution suitable for the growth of nitrobacteria, and the preparation speed of the high-activity nitrobacteria biofilm is increased.
Drawings
FIG. 1 is a photograph of a suspension carrier before preparation of a biofilm.
FIG. 2 is a picture of a suspended carrier after a high-activity nitrifying bacteria biofilm is prepared by the method.
FIG. 3 is a graph showing the effect of a 100mL suspension carrier high-activity nitrifying bacteria biofilm prepared by the present invention on removal of ammonia nitrogen from aquaculture pond water.
Detailed Description
The invention aims to provide a preparation method of a high-activity nitrifying bacteria biomembrane for aquaculture water treatment, which achieves the purposes of promoting the growth of biomembrane nitrifying bacteria, accelerating the preparation speed of the high-activity nitrifying bacteria biomembrane and improving the nitrifying activity, and solves the problem of low ammonia nitrogen removal rate of an aquaculture water body. The method takes a suspended carrier as a microbial carrier, improves the nitrification activity of nitrifying bacteria by loading the biofilm with iron, can adapt to a large-range temperature condition of 5-35 ℃, is suitable for mariculture and freshwater culture, can greatly improve the fixing speed of the nitrifying bacteria biofilm, and can quickly remove ammonia nitrogen and nitrite nitrogen in water, and the attached nitrifying bacteria biofilm has high-efficiency nitrification activity.
The preparation method of the nitrifying bacteria biomembrane specifically comprises the following steps.
(1) The reactor was charged with a mixture of seaside fine sand and aquaculture pond water, the volume of sea sand being 1/3 of the reactor volume (mixture).
(2) Adding 300-500 mg of MgSO (MgSO) into every 1000mL of distilled water4·H2O, 20-50 mg of CaCl2·H2O, 0.2-2.0 mg of MnCl2·H2O, 0.1-0.2 mg of Na2 MoO4·H2O, 0.1-0.2 mg CoCl2·H2O, 0.1-0.2 mg ZnSO4·H2O and 80-500 mg of K2HPO4Mixing uniformly to prepare the nutrient solution.
And adding 1-2% of nutrient solution into the mixture in the reactor, and forming a mixed solution together with the mixture.
Mg, Ca, Mn, Na, Co, Zn and K, especially Mn, Co and Zn, are essential trace elements for some efficient nitrobacteria strains, and can avoid weak competition position caused by lack of the trace elements.
(3) Aerating the mixed solution in the reactor, and controlling the concentration of dissolved oxygen to be more than 6 mg/L;
(4) adding an iron hydroxide solution into the mixed liquid of the reactor, wherein the adding amount of the iron hydroxide solution is 20-40 mg of Fe3+/L。
Adding the ferric hydroxide solution immediately after the preparation, wherein the preparation method comprises the steps of adding the sodium hydroxide solution into the 15% ferric trichloride solution, continuously stirring, simultaneously measuring the pH value of the mixed solution, stopping adding the sodium hydroxide solution until the pH value is 7-8, and adding the sodium hydroxide solution into a reactor while continuously stirring.
(5) And regularly monitoring the ammonia nitrogen concentration of the mixed liquid in the reactor, and adding ammonium sulfate or ammonium chloride to keep the ammonia nitrogen concentration at 2-20 mg/L all the time.
(6) And (3) regularly monitoring the pH of the mixed liquid in the reactor, and adding sodium bicarbonate to keep the pH at 7-9 all the time.
(7) The concentration of suspended matter in the mixed liquid in the reactor except sea sand is monitored every day, and when the concentration of suspended matter is increased by 30% or more than the previous day, the suspended carrier is added. Commercially available suspension carriers are mainly based on a density close to that of water, i.e. the density of the suspension carrier is 0.94-0.97 g/cm3。
(8) The aeration amount is adjusted to be small so that the dissolved oxygen concentration is 2.5-4.5 mg/L.
(9) Periodically observing the state of the suspended carrier, stopping aeration when more than 20% of the suspended carrier in the floating state changes from the floating state to a rolling state along with the water flow, taking out the sea sand, and continuously keeping the supernatant and the suspended carrier in the reactor.
(10) And starting aeration, maintaining the dissolved oxygen concentration at 2.5-4.5 mg/L, adding ammonium sulfate or ammonium chloride to supplement ammonia nitrogen concentration at 20mg/L, and then not supplementing ammonia nitrogen.
(11) And when the ammonia nitrogen can not be detected, the preparation of the biological membrane is finished.
Examples are given below.
The preparation process of the nutrient solution comprises the following steps: 400mg of MgSO (MgSO) were added to every 1000mL of distilled water4·H2O, 35mg of CaCl2·H2O, 1.0mg of MnCl2·H2O, 0.15mg of Na2 MoO4·H2O, 0.15mg CoCl2·H2O, 0.15mg of ZnSO4·H2O and 300mg of K2HPO4And stirring and mixing uniformly.
The suspension vehicle used is shown in FIG. 1.
(1) The reactor is filled with seaside fine sand and aquaculture pond water, and the volume of the sea sand is 1/3 of the volume of the reactor.
(2) 1.5 percent of nutrient solution is added into the reactor.
(3) Aerating the mixed solution in the reactor, and controlling the concentration of dissolved oxygen to be between 6mg/L and 8 mg/L;
(4) adding ferric hydroxide solution into the mixed solution of the reactor, wherein the adding amount is 30mg of Fe3+And L. Specifically, adding a sodium hydroxide solution into a 15% ferric trichloride solution, stirring the mixture continuously until the pH value of the mixed solution is 7-8, and then adding the mixed solution into a reactor while stirring.
(5) And regularly monitoring the ammonia nitrogen concentration of the mixed liquid in the reactor, and keeping the ammonia nitrogen concentration at 12mg/L all the time by adding ammonium sulfate or ammonium chloride.
(6) And (3) regularly monitoring the pH of the mixed liquid in the reactor, and adding sodium bicarbonate to keep the pH at 7-9 all the time.
(7) Monitoring the concentration of suspended matters except sea sand in the mixed liquid in the reactor every day, and adding the mixed liquid with the density of 0.95g/cm when the concentration of the suspended matters is increased by 30 percent or more than that of the mixed liquid in the previous day3The suspension carrier of (1).
(8) The aeration amount is adjusted to be small so that the dissolved oxygen concentration is 2.5-4.5 mg/L.
(9) And regularly observing the state of the suspended carriers, stopping aeration when more than 20% of the suspended carriers in the floating state change from the floating state to a rolling state along with the water flow, taking out the sea sand, and continuously keeping the supernatant and the suspended carriers in the reactor.
(10) And starting aeration, maintaining the dissolved oxygen concentration at 3.5mg/L, adding ammonium sulfate or ammonium chloride to supplement ammonia nitrogen concentration at 20mg/L, and then not supplementing ammonia nitrogen.
(11) And when ammonia nitrogen can not be detected, the preparation of the biological membrane is finished.
The high-activity nitrifying bacteria biofilm (suspension carrier) prepared by the above process is shown in fig. 2. The effect of the prepared high-activity nitrifying bacteria biomembrane with 100mL of suspension carriers on removing ammonia nitrogen in water in the aquaculture pond is shown in figure 3, and the more suspension carriers are, the more ammonia nitrogen is removed.

Claims (6)

1. A preparation method of a high-activity nitrifying bacteria biomembrane for aquaculture water treatment is characterized by comprising the following steps:
(1) adding sea sand into the water in the aquaculture pond to form a mixture, wherein the volume of the sea sand is not more than 1/3 of the total volume of the mixture;
(2) adding 1-2% of nutrient solution into the mixture according to the volume ratio to form a mixed solution;
(3) aerating the mixed solution, and controlling the concentration of dissolved oxygen to be more than 6 mg/L;
(4) adding iron hydroxide solution with the addition amount of 20-40 mg Fe3+/L；
(5) Regularly monitoring the ammonia nitrogen concentration and the pH value of the mixed solution, so that the ammonia nitrogen concentration is always maintained at 2-20 mg/L, and the pH value is always maintained at 7-9;
(6) monitoring the concentration of suspended matters except sea sand in the mixed solution every day, and adding a suspended carrier when the concentration of the suspended matters is increased by 30% or more than that of the suspended matters in the mixed solution in the previous day;
(7) adjusting aeration quantity to ensure that the concentration of dissolved oxygen is 2.5-4.5 mg/L;
(8) regularly observing the state of the suspended carriers, and stopping aeration and taking out the sea sand when more than 20% of the suspended carriers in the floating state change from the floating state to the rolling state along with the water flow;
(9) starting aeration, maintaining the dissolved oxygen concentration at 2.5-4.5 mg/L, adjusting the ammonia nitrogen concentration to 20mg/L, and then not supplementing ammonia nitrogen;
(10) and when ammonia nitrogen can not be detected, the preparation of the biological membrane is finished.
2. The method for preparing the high-activity nitrifying bacteria biofilm for the aquaculture water treatment as claimed in claim 1, wherein the preparation process of the nutrient solution in the step (2) is as follows: adding 300-500 mg of MgSO (MgSO) into every 1000mL of distilled water4·H2O, 20-50 mg of CaCl2·H2O, 0.2-2.0 mg of MnCl2·H2O, 0.1-0.2 mg of Na2MoO4·H2O, 0.1-0.2 mg CoCl2·H2O, 0.1-0.2 mg ZnSO4·H2O and 80-500 mg of K2HPO4And mixing uniformly.
3. The preparation method of the high-activity nitrifying bacteria biofilm for aquaculture water treatment as claimed in claim 1, wherein the ferric hydroxide solution in step (4) is added immediately after the preparation is completed, and the preparation process comprises the steps of adding the sodium hydroxide solution into the ferric trichloride solution with the mass concentration of 15% and continuously stirring until the pH value is 7-8, and stopping adding the sodium hydroxide solution while continuously stirring and adding the sodium hydroxide solution into the mixed solution.
4. The method for preparing the high-activity nitrifying bacteria biofilm for aquaculture water treatment according to claim 1, wherein the ammonia nitrogen concentration in the step (5) is maintained at 2-20 mg/L by adding ammonium sulfate or ammonium chloride.
5. The preparation method of the high-activity nitrifying bacteria biofilm for aquaculture water treatment as claimed in claim 1, wherein in the step (5), the pH is maintained at 7-9 by adding sodium bicarbonate.
6. The method for preparing the high-activity nitrifying bacteria biofilm for aquaculture water treatment as claimed in claim 1, wherein the adding density of the suspension carrier in the step (6) is 0.94-0.97 g/cm3。
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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110253625A1 (en) * 2008-12-28 2011-10-20 Metawater Co., Ltd. Method and device for removing biological nitrogen and support therefor
US20110284461A1 (en) * 2010-05-20 2011-11-24 Otv Sa Controlled Aeration of Integrated Fixed-Film Activated Sludge Bioreactor Systems for the Treatment of Wastewater
CN102502949A (en) * 2011-11-02 2012-06-20 青岛理工大学 Method for improving activity of nitrification function microbiology in activated sludge through feeding iron hydroxide during in situ preparation
CN104961229A (en) * 2015-07-09 2015-10-07 南京瑞利克斯环境科技有限公司 Nitrifying bacterium microbial film and preparing method of nitrifying bacterium microbial film
CN104973679A (en) * 2015-07-16 2015-10-14 通威股份有限公司 Quick start method of biofilter in low temperature environment
CN110803766A (en) * 2019-11-05 2020-02-18 大连理工大学 Synchronous nitrification and denitrification process combining nitrification functional type and denitrification functional type suspension carrier

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110253625A1 (en) * 2008-12-28 2011-10-20 Metawater Co., Ltd. Method and device for removing biological nitrogen and support therefor
US20110284461A1 (en) * 2010-05-20 2011-11-24 Otv Sa Controlled Aeration of Integrated Fixed-Film Activated Sludge Bioreactor Systems for the Treatment of Wastewater
CN102502949A (en) * 2011-11-02 2012-06-20 青岛理工大学 Method for improving activity of nitrification function microbiology in activated sludge through feeding iron hydroxide during in situ preparation
CN104961229A (en) * 2015-07-09 2015-10-07 南京瑞利克斯环境科技有限公司 Nitrifying bacterium microbial film and preparing method of nitrifying bacterium microbial film
CN104973679A (en) * 2015-07-16 2015-10-14 通威股份有限公司 Quick start method of biofilter in low temperature environment
CN110803766A (en) * 2019-11-05 2020-02-18 大连理工大学 Synchronous nitrification and denitrification process combining nitrification functional type and denitrification functional type suspension carrier

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