CN114669338A - Micro-fluidic chip based on urine detects disease - Google Patents
Micro-fluidic chip based on urine detects disease Download PDFInfo
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- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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Abstract
The invention discloses a micro-fluidic chip for detecting diseases based on urine, which comprises an upper substrate layer, wherein an upper liquid inlet is formed in the upper substrate layer; the middle constant-temperature reaction layer comprises a first constant-temperature reaction chip body, the first constant-temperature reaction chip body is arranged on the lower side of the upper substrate layer, the lower side of the first constant-temperature reaction chip body is connected with a second constant-temperature reaction chip body, a detection reaction channel is arranged between the first constant-temperature reaction chip body and the second constant-temperature reaction chip body, a detection liquid storage tank and a sample injection liquid storage tank which can be communicated with the detection reaction channel are arranged on the middle constant-temperature reaction layer, and at least one heating groove which can heat the detection reaction channel and is isolated from the detection reaction channel is arranged on the middle constant-temperature reaction layer; the lower substrate layer is connected to the lower side of the second constant-temperature reaction chip body; the invention realizes the detection reaction of the mixed solution under the condition of constant temperature and improves the detection accuracy.
Description
Technical Field
The invention relates to the technical field of urine detection, in particular to a micro-fluidic chip for detecting diseases based on urine.
Background
Microfluidics refers to the science and technology involved in systems that process or manipulate tiny fluids (nanoliters to attoliters in volume) using microchannels (tens to hundreds of microns in size), and is an emerging interdiscipline that involves chemical, fluid physics, microelectronics, new materials, biology, and biomedical engineering. Because of their miniaturization, integration, etc., microfluidic devices are commonly referred to as microfluidic chips, also known as lab-on-a-chip and micro total analysis systems. The early concept of microfluidics can be traced back to gas chromatographs fabricated on silicon wafers by photolithography in the 70 s of the 19 th century, and then developed into microfluidic capillary electrophoresis instruments, microreactors and the like. One of the important features of microfluidics is the unique fluid properties in microscale environments, such as laminar flow and droplets. With these unique fluidic phenomena, microfluidics can achieve a range of microfabrication and micromanipulation that are difficult to accomplish with conventional methods. Microfluidics is currently considered to have great development potential and broad application prospects in biomedical research.
The urine detection technology adopts HPLC (tumor marker) to measure the p-hydroxyphenylalanine, and shows that the content of the p-hydroxyphenylalanine in urine of malignant tumor patients is obviously increased by 50-150%. Research proves that in the initial stage of tumorigenesis, the synthetases of amine substances such as tyrosine hydroxylase, dopamine hydroxylase, phenylethanolamine methyltransferase and the like of tumor cells are higher than those of normal tissues, so that a certain amount of urine is increased, and the phenomenon of digestive tract tumor is particularly obvious. In the tumor formation stage, the corresponding stress response of the human body also leads to the vigorous metabolism of a large amount of amine substances due to the invasion of tumor cells, and the corresponding increase is also shown in urine. Therefore, whether the relevant cell abnormal metabolism exists in the body of the tested person can be judged by detecting the content of the p-hydroxyphenylalanine in the urine. In the detection reaction process, the reaction temperature needs to be maintained within a certain range, the detection environment is harsh, the reaction of urine in a proper temperature range is difficult to ensure, and the accuracy of the analysis of the detection result is reduced.
Disclosure of Invention
This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. In this section, as well as in the abstract and the title of the invention of this application, simplifications or omissions may be made to avoid obscuring the purpose of the section, the abstract and the title, and such simplifications or omissions are not intended to limit the scope of the invention.
The present invention has been made in view of the above and/or other problems occurring in the prior art urine tests.
Therefore, the present invention is directed to a micro fluidic chip for detecting diseases based on urine, which has a compact structure and is easy to perform the reaction between urine and a detection solution at a suitable temperature.
In order to solve the technical problems, the invention provides the following technical scheme: a micro-fluidic chip for detecting diseases based on urine comprises,
the upper substrate layer is provided with an upper liquid inlet;
the middle constant-temperature reaction layer comprises a first constant-temperature reaction chip body, the first constant-temperature reaction chip body is arranged on the lower side of the upper substrate layer, a second constant-temperature reaction chip body is connected to the lower side of the first constant-temperature reaction chip body, a detection reaction channel is arranged between the first constant-temperature reaction chip body and the second constant-temperature reaction chip body, a detection liquid storage tank and a sample injection liquid storage tank which can be communicated with the detection reaction channel are arranged on the middle constant-temperature reaction layer, and at least one heating groove which can heat the detection reaction channel and is isolated from the detection reaction channel is arranged on the middle constant-temperature reaction layer;
and the lower substrate layer is connected to the lower side of the second constant-temperature reaction chip body.
As a preferable embodiment of the micro-fluidic chip for detecting diseases based on urine of the present invention, wherein: the one side that first constant temperature reaction chip body faced down is equipped with the heavy groove of first detection reaction, and the one side that second constant temperature reaction chip body faced up is equipped with the heavy groove of second detection reaction that corresponds with the heavy groove of first detection reaction, first detection reaction is formed between heavy groove and the heavy groove of second detection reaction detect the reaction channel.
As a preferable embodiment of the micro-fluidic chip for detecting diseases based on urine of the present invention, wherein: the first constant temperature reaction chip body is provided with a lower liquid inlet, the upward end of the second constant temperature reaction chip body is provided with a first liquid storage settling tank, a sample liquid storage tank is formed among the upper liquid inlet, the lower liquid inlet and the first liquid storage settling tank, and a first liquid outlet settling tank is arranged on the second constant temperature reaction chip body between the first liquid storage settling tank and one end of the second detection reaction settling tank.
As a preferable embodiment of the micro-fluidic chip for detecting diseases based on urine of the present invention, wherein: the first constant temperature reaction chip body is provided with a detection liquid storage port, one upward end of the second constant temperature reaction chip body is provided with a second liquid storage sink, a detection liquid storage pool is formed between the detection liquid storage port and the second liquid storage sink, and a second liquid outlet sink is arranged on the second constant temperature reaction chip body between the second liquid storage sink and one end of the second detection reaction sink.
As a preferable embodiment of the micro-fluidic chip for detecting diseases based on urine of the present invention, wherein: a sample injection liquid control valve plate and a detection liquid control valve plate are connected between the first constant temperature reaction chip body and the second constant temperature reaction chip body in a sliding mode, the sample injection liquid control valve plate is provided with a sample injection liquid control hole enabling the first liquid storage sinking groove and one end of the second detection reaction sinking groove to be communicated, and the detection liquid control valve plate is provided with a detection liquid control hole enabling the second liquid storage sinking groove and one end of the second detection reaction sinking groove to be communicated.
As a preferable scheme of the micro-fluidic chip for detecting diseases based on urine, the invention comprises: the device comprises a first constant temperature reaction chip body, a second constant temperature reaction chip body, a first control switch, a second control switch, a sample injection liquid control valve plate and a detection liquid control valve plate, wherein the downward side of the first constant temperature reaction chip body is provided with a first upper connection sinking groove and a second upper connection sinking groove, the upward side of the second constant temperature reaction chip body is connected with the first control switch and the second control switch, the upper end of the first control switch is connected with the first upper connection sinking groove, the upper end of the second control switch is connected with the second upper connection sinking groove, and the first control switch and the second control switch respectively control the sliding of the sample injection liquid control valve plate and the detection liquid control valve plate.
As a preferable embodiment of the micro-fluidic chip for detecting diseases based on urine of the present invention, wherein: one end that second constant temperature reaction chip body is down is equipped with the heating bath, be equipped with the heater strip in the heating bath, a plurality of first heat conduction liquid inlets have arranged on the first constant temperature reaction chip body, a plurality of and first heat conduction liquid inlet one-to-one's second heat conduction liquid inlets have arranged on the second constant temperature reaction chip body in the heating bath outside.
As a preferable embodiment of the micro-fluidic chip for detecting diseases based on urine of the present invention, wherein: the utility model discloses a heating device, including heating tank, insulator, conducting rod, reset spring, heating tank, heating control conducting strip, be equipped with heat sensitive liquid apotheca on the second constant temperature reaction chip body of heating tank department, be connected with the conducting rod on the outer second constant temperature reaction chip body of heating tank slidable, one side that the relative heating tank of conducting rod set up is equipped with the insulator spindle, the insulator spindle is kept away from the one end of conducting rod and is filled in heat sensitive liquid apotheca, the cover is equipped with insulating reset spring on the insulator spindle, reset spring's one end is connected on second constant temperature reaction chip body, and reset spring's the other end is connected in one side that the relative heating tank of conducting rod set up, all be connected with the heating control conducting strip on the second constant temperature reaction chip body of conducting rod both sides.
As a preferable scheme of the micro-fluidic chip for detecting diseases based on urine, the invention comprises: still all be connected with out liquid control conducting strip on the second constant temperature reaction chip body of conducting rod both sides, the conducting rod with go out liquid control conducting strip contact and break away from when heating control conducting strip, the heater strip stops the heating, when conducting rod both sides and play liquid control conducting strip contact, first control switch and second control switch control respectively advance kind liquid control valve piece and detect liquid control valve piece and remove, make the heavy groove of first feed liquor heavy groove and the heavy groove intercommunication of first detection reaction, the heavy groove of second feed liquor heavy groove and the heavy groove intercommunication of second detection reaction.
As a preferable embodiment of the micro-fluidic chip for detecting diseases based on urine of the present invention, wherein: the first constant temperature reaction chip body at the other end of the first detection reaction sink tank is provided with a waste liquid port, the second constant temperature reaction chip body at the other end of the second detection reaction sink tank is provided with a waste liquid sink tank, and the waste liquid port and the waste liquid sink tank form a waste liquid storage tank.
The invention has the beneficial effects that: when detecting, the heating wire heats the heat-conducting liquid in the heating groove, the heat-sensitive liquid in the heat-sensitive liquid storage chamber expands when heated, the insulating rod is pushed to move outwards after overcoming the elasticity of the reset spring, when the conducting rod moves to be contacted with the liquid outlet control conducting strip, the first control switch and the second control switch are electrified to drive the sample liquid control valve plate and the sample liquid outlet control valve plate to move, so that the sample liquid storage tank is communicated with the reaction channel through the sample liquid control hole, the detection liquid storage tank is communicated with the reaction channel through the detection liquid control hole, the detection liquid in the detection liquid storage tank and the sample liquid in the sample liquid storage tank simultaneously enter the reaction channel for reaction, when the conducting rod is contacted with the liquid outlet control conducting strip, the heating wire is controlled to stop heating, so that the mixed solution in the reaction channel reacts at constant temperature, the detection reaction at specific temperature is realized, and the dynamic regulation and control of the temperature in the reaction process is realized, the method is favorable for the accuracy of the detection result of the solution after reaction.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive exercise. Wherein:
fig. 1 is a front view of the present invention.
Fig. 2 is a first exploded view of the present invention.
Fig. 3 is an exploded view of the second embodiment of the present invention.
Fig. 4 is a partially enlarged view of a portion a in fig. 3.
Fig. 5 is an exploded view of the third embodiment of the present invention.
Fig. 6 is a partially enlarged view of fig. 5 at B.
In the figure, 100 lower substrate layer, 200 upper substrate layer, 201 upper liquid inlet, 300 first constant temperature reaction chip body, 301 waste liquid inlet, 302 upper heating tank, 303 lower liquid inlet, 304 detection liquid storage port, 305 first heat-transfer liquid inlet, 306 first detection reaction sink, 400 second constant temperature reaction chip body, 401 second heat-transfer liquid inlet, 402 second detection reaction sink, 403 waste liquid sink, 404 first liquid storage sink, 405 second liquid storage sink, 406 lower heating tank, 407 first connection sink, 408 second connection sink, 409 second liquid outlet sink, 410 first liquid outlet sink, 411 heat-sensitive liquid storage chamber, 500 liquid inlet control component, 501 first control switch, 502 first metal sheet, 503 sample liquid control valve sheet, 503a sample liquid control hole, 504 second control switch, 505 second metal sheet, 506 detection liquid control valve sheet, 506a detection liquid control hole, 600 conduction module, 601 insulating rod, 602 conducting rod, 700 heating control conducting strip, 800 liquid outlet control conducting strip, 900 reset spring and 1000 sealing strip.
Detailed Description
Before the technical solution of the present invention is explained, the terms used herein are defined as follows:
in order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.
Furthermore, the references herein to "one embodiment" or "an embodiment" refer to a particular feature, structure, or characteristic that may be included in at least one implementation of the present invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.
Example 1
Referring to fig. 1 and fig. 2, this embodiment provides a micro-fluidic chip for disease detection based on urine, which integrates urine transportation and collection and disease detection to facilitate disease detection, according to a first embodiment of the present invention.
A micro-fluidic chip for detecting diseases based on urine comprises an upper substrate layer 200, wherein an upper liquid inlet 201 is formed in the upper substrate layer 200;
the middle constant-temperature reaction layer comprises a first constant-temperature reaction chip body 300, the first constant-temperature reaction chip body 300 is arranged on the lower side of the upper substrate layer 200, a second constant-temperature reaction chip body 400 is connected to the lower side of the first constant-temperature reaction chip body 300, a detection reaction channel is arranged between the first constant-temperature reaction chip body 300 and the second constant-temperature reaction chip body 400, a detection liquid storage tank and a sample injection liquid storage tank which can be communicated with the detection reaction channel are arranged on the middle constant-temperature reaction layer, and at least one heating groove which can heat the detection reaction channel and is isolated from the detection reaction channel is arranged on the middle constant-temperature reaction layer;
a lower substrate layer 100, the lower substrate layer 100 being attached to the lower side of the second isothermal reaction chip body 400.
Further, a first detection reaction sink groove 306 in a curve shape is arranged on the downward side of the first constant temperature reaction chip body 300, a second detection reaction sink groove 402 corresponding to the first detection reaction sink groove 306 is arranged on the upward side of the second constant temperature reaction chip body 400, and a detection reaction channel is formed between the first detection reaction sink groove 306 and the second detection reaction sink groove 402; a lower liquid inlet 303 is formed on the first constant temperature reaction chip body 300, a first liquid storage sink tank 404 is formed at the upward end of the second constant temperature reaction chip body 400, a sample liquid storage pool is formed among the upper liquid inlet 201, the lower liquid inlet 303 and the first liquid storage sink tank 404, and a first liquid outlet sink tank 410 is arranged on the second constant temperature reaction chip body 400 between the first liquid storage sink tank 404 and one end of the second detection reaction sink tank 402; the first constant temperature reaction chip body 300 is provided with a detection liquid storage port 304, the upward end of the second constant temperature reaction chip body 400 is provided with a second liquid storage sink tank 405, a detection liquid storage pool is formed between the detection liquid storage port 304 and the second liquid storage sink tank 405, and a second liquid outlet sink tank 409 is arranged on the second constant temperature reaction chip body 400 between the second liquid storage sink tank 405 and one end of the second detection reaction sink tank 402.
Further, a liquid inlet control assembly 500 for controlling the first liquid storage sink tank 404 and the second liquid storage sink tank 405 is connected between the first constant temperature reaction chip body 300 and the second constant temperature reaction chip body 400, the liquid inlet control assembly 500 comprises a liquid inlet control valve plate 503 and a liquid detection control valve plate 506 slidably connected between the first constant temperature reaction chip body and the second constant temperature reaction chip body 400, the liquid inlet control valve plate 503 is provided with a liquid inlet control hole 503a for communicating one end of the first liquid storage sink tank 404 with one end of the second liquid detection reaction sink tank 402, the liquid detection control valve plate 506 is provided with a liquid detection control hole 506a, when the liquid inlet control hole 503a moves to the position of the first liquid outlet sink tank 410, the first liquid storage sedimentation tank 404 is communicated with one end of the detection reaction channel, and when the detection liquid control hole 506a moves to the position of the second liquid outlet sedimentation tank 409, the second liquid storage sedimentation tank 405 is communicated with one end of the detection reaction channel; a first control switch 501 and a second control switch 504 are fixedly connected to the upward side of the second isothermal reaction chip body 400, in this embodiment, the first control switch 501 and the second control switch 504 are preferably electromagnets, the downward side of the first isothermal reaction chip body 300 is provided with a first connection sinking groove 407 and a second connection sinking groove 408, the upper end of the first control switch 501 is in the first connection sinking groove 407, the upper end of the second control switch 504 is in the first isothermal reaction chip body, the first control switch 501 and the second control switch 504 respectively control the sliding of the sample liquid control valve plate 503 and the detection liquid control valve plate 506, specifically, a first metal plate 502 sliding along the second isothermal reaction chip body 400 is fixed to the side of the sample liquid control valve plate 503 opposite to the first control switch 501, a second metal plate 505 sliding along the second isothermal reaction chip body 400 is fixed to the side of the detection liquid control valve plate 506 opposite to the second control switch 504, when the first control switch 501 and the second control switch 504 are powered on, the injection liquid control valve plate 503 and the detection liquid control valve plate 506 are pushed to move under the action of repulsion force, the injection liquid storage pool is communicated with the detection reaction channel through the injection liquid control hole 503a, the detection liquid storage pool is communicated with the detection reaction channel through the detection liquid control hole 506a, liquid feeding is finished, the first control switch 501 and the second control switch 504 are powered off, the injection liquid control valve plate 503 and the detection liquid control valve plate 506 are driven to move under the action of attraction force, and the injection liquid storage pool and the detection reaction channel are isolated.
In order to discharge redundant solution, a waste liquid port 301 is formed in the first constant temperature reaction chip body 300 at the other end of the first detection reaction settling tank 306, a waste liquid settling tank 403 is formed in the second constant temperature reaction chip body 400 at the other end of the second detection reaction settling tank 402, and the waste liquid port 301 and the waste liquid settling tank 403 form a waste liquid storage tank; and (4) allowing the redundant waste liquid to enter a waste liquid storage pool.
The sample solution in this embodiment is a urine sample, after the reaction is finished, HPLC is used to detect the content of p-hydroxyphenylalanine in the urine sample by using a tumor marker in the detection solution, the content of p-hydroxyphenylalanine in the urine sample is reflected by the color of the precipitate generated by the reaction, a spectrophotometer is used to analyze the color of the generated precipitate, and the color is compared with a set color numerical range to obtain a detection result.
Example 2
Referring to fig. 4 to 6, a second embodiment of the present invention is different from the second embodiment in that a microfluidic chip for detecting a disease based on urine is provided, and the present embodiment can further realize liquid inlet and heating control.
The utility model provides a micro-fluidic chip based on urine detects disease, wherein, the one end that second constant temperature reaction chip body 400 was down is equipped with down heating tank 406, is equipped with the heater strip in the heating tank 406 down, and the heater strip in this application is not drawn, and it is prior art, has arranged a plurality of first heat conduction liquid inlet 305 on the first constant temperature reaction chip body 300, has arranged a plurality of and first heat conduction liquid inlet 305 one-to-one's second heat conduction liquid inlet 401 on the second constant temperature reaction chip body 400 outside lower heating tank 406.
Further, a heat sensitive liquid storage chamber 411 is arranged on the second constant temperature reaction chip body 400 at the lower heating tank 406, a heat sensitive liquid injection port is arranged on the heat sensitive liquid storage chamber 411, a sealing plate 1000 is connected at the heat sensitive liquid injection port, a conducting module 600 is slidably connected to the second constant temperature reaction chip body 400 outside the lower heating tank 406, the conducting module 600 comprises a conducting rod 602, an insulating rod 601 is arranged at one side of the conducting rod 602 corresponding to the lower heating tank 406, a plug head plugged into the heat sensitive liquid storage chamber 411 is arranged at one end of the insulating rod 601 far away from the conducting rod 602, the plug head is always arranged in the heat sensitive liquid storage chamber 411, an insulating return spring 900 is sleeved on the insulating rod 601, one end of the return spring 900 is connected to the second constant temperature reaction chip body 400, the other end of the return spring 900 is connected to one side of the conducting rod 602 corresponding to the lower heating tank 406, the second constant temperature reaction chip body 400 on both sides of the conductive rod 602 is connected with a heating control conductive sheet 700, and the first control switch 501 and the second control switch 504 respectively control the movement of the sample inlet control valve sheet 503 and the detection liquid control valve sheet 506, so that the first liquid inlet sink tank is communicated with the first detection reaction sink tank 306, and the second liquid inlet sink tank is communicated with the second detection reaction sink tank 402.
In an initial state, both ends of the conductive rod 602 are in contact with the heating control conductive sheet 700, and the return spring 900 is in a natural state; before the sealing plate 1000 is installed, the conductive rod 602 is fixed, the heat sensitive liquid is injected into the heat sensitive liquid storage chamber 411 through the heat sensitive liquid injection port, and after the injection is finished, the sealing plate 1000 is welded on the outer edge of the heat sensitive liquid storage chamber 411, and the conductive rod 602 is loosened.
In order to further realize real-time regulation and control of the temperature, the second constant temperature reaction chip bodies 400 on both sides of the conductive rod 602 are also connected with the liquid outlet control conductive sheet 800, and when the conductive rod 602 is contacted with the liquid outlet control conductive sheet 800, the heating wire stops heating.
In an initial state, when the device is used, the outer sides of the two heating control conductive sheets 700 are provided with leads, the two leads are respectively and electrically connected with a controller and a power supply which are arranged outside, the heating wires heat the heat-conducting liquid in the lower heating groove 406, the heat-sensitive liquid in the heat-sensitive liquid storage chamber 411 is heated to expand, after overcoming the elasticity of the reset spring 900, the insulating rod 601 is pushed to move outwards, when the conductive rod 602 moves to be contacted with the liquid outlet control conductive sheet 800, the temperature is heated to be required, the controller controls the first control switch 501 and the second control switch 504 to be electrified to drive the sample liquid control valve plate 503 and the detection liquid control valve plate 506 to move, so that the sample liquid storage tank is communicated with the detection reaction channel through the sample liquid control hole 503a, the detection liquid storage tank is communicated with the detection reaction channel through the detection liquid control hole 506a, the detection liquid in the detection liquid storage tank and the sample liquid in the sample liquid storage tank simultaneously enter the detection reaction channel, the temperature is gradually increased under the action of the heat-conducting liquid.
The outer sides of the two liquid outlet control conductive sheets 800 are respectively electrically connected with a controller and a power supply, when the conductive rod 602 is in contact with the liquid outlet control conductive sheet 800, the insulating rod is in contact with the heating control conductive sheet 700, which indicates that the temperature in the lower heating tank 406 is heated to a required temperature, the liquid outlet control conductive sheets 800 are conducted, a conduction signal between the liquid outlet control conductive sheets 800 is sent to the controller, the controller controls the heating wire to stop heating, and simultaneously controls the first control switch 501 and the second control switch 504 to be electrified, the sample injection liquid control valve sheet 503 and the detection liquid control valve sheet 506 respectively move under the repulsive force of the first control switch 501 and the second control switch 504, so that the sample injection liquid control hole 503a moves to the first liquid outlet sink 410, the detection liquid control hole 506a moves to the second liquid outlet sink 409, and a mixed solution entering the detection reaction channel reacts in the detection reaction channel, when the temperature drops, the volume of the heat sensitive liquid shrinks, the conductive rod 602 contacts with the heating control conductive sheet 400 again, the controller continues to control the heating wire to heat, so that the heat conductive liquid in the lower heating groove 406 is kept at a constant temperature, the temperature in the reaction process is dynamically regulated, the solution in the detection reaction channel is ensured to react in a constant temperature environment, and the accuracy of the subsequent detection result analysis is improved.
Example 3
Referring to fig. 3, a third embodiment of the present invention is different from the third embodiment in that a microfluidic chip for disease detection based on urine is provided, and this embodiment can further ensure that a solution reacts in a constant temperature environment.
A micro-fluidic chip for detecting diseases based on urine is characterized in that an upper heating groove 302 is formed in one upward end of a first constant-temperature reaction chip body 300, a detection reaction channel is arranged between the upper heating groove 302 and a lower heating groove 406, heating wires are arranged in the upper heating groove 302 and the lower heating groove 406, a first heat conduction liquid inlet 305 is arranged at the outer edge of the upper heating groove 302, and the heating wires in the embodiment are heating resistance wires.
The heat conducting liquid in the upper heating groove 302 and the lower heating groove 303 is heated and simultaneously transfers heat to the mixed solution in the detection reaction channel, so that the heat retaining property of the mixed solution in the reaction channel is improved.
It should be noted that the above-mentioned embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the claims of the present invention.
Claims (10)
1. A micro-fluidic chip based on urine detects disease which characterized in that: which comprises
The upper substrate layer (200), wherein an upper liquid inlet (201) is formed on the upper substrate layer (200);
the middle constant-temperature reaction layer comprises a first constant-temperature reaction chip body (300), the first constant-temperature reaction chip body (300) is arranged on the lower side of the upper substrate layer (200), a second constant-temperature reaction chip body (400) is connected to the lower side of the first constant-temperature reaction chip body (300), a detection reaction channel is arranged between the first constant-temperature reaction chip body (300) and the second constant-temperature reaction chip body (400), a detection liquid storage pool and a sample injection liquid storage pool which can be communicated with the detection reaction channel are arranged on the middle constant-temperature reaction layer, and at least one heating groove which can heat the detection reaction channel and is isolated from the detection reaction channel is arranged on the middle constant-temperature reaction layer;
a lower substrate layer (100), wherein the lower substrate layer (100) is connected to the lower side of the second constant temperature reaction chip body (400).
2. The micro fluidic chip for detecting diseases based on urine as claimed in claim 1, wherein: one side that first constant temperature reaction chip body (300) down is equipped with first detection reaction heavy groove (306), and one side that second constant temperature reaction chip body (400) up is equipped with and detects reaction heavy groove (402) with the second that first detection reaction heavy groove (306) correspond, first detection reaction heavy groove (306) and second detect and form between the reaction heavy groove (402) detect the reaction passageway.
3. The micro fluidic chip for detecting diseases based on urine as claimed in claim 2, wherein: first isothermal reaction chip body (300) go up to open there is lower inlet (303), second isothermal reaction chip body (400) one end up is opened has first stock solution heavy groove (404), go up and form between feed inlet (201), lower inlet (303) and first stock solution heavy groove (404) and advance a kind liquid storage pool, be equipped with first play liquid heavy groove (410) on second isothermal reaction chip body (400) between first stock solution heavy groove (404) and second detection reaction heavy groove (402) one end.
4. The microfluidic chip for detecting diseases according to claim 2 or 3, wherein: the detection device is characterized in that a detection liquid storage port (304) is formed in the first constant-temperature reaction chip body (300), a second liquid storage sink groove (405) is formed in one upward end of the second constant-temperature reaction chip body (400), a detection liquid storage pool is formed between the detection liquid storage port (304) and the second liquid storage sink groove (405), and a second liquid outlet sink groove (409) is formed in the second constant-temperature reaction chip body (400) between the second liquid storage sink groove (405) and one end of the second detection reaction sink groove (402).
5. The micro fluidic chip for detecting diseases based on urine as claimed in claim 4, wherein: a sample injection liquid control valve plate (503) and a detection liquid control valve plate (506) are slidably connected between the first constant temperature reaction chip body (300) and the second constant temperature reaction chip body (400), the sample injection liquid control valve plate (503) is provided with a sample injection liquid control hole (503 a) which enables one end of the first liquid storage sink tank (404) to be communicated with one end of the second detection reaction sink tank (402), and the detection liquid control hole (506 a) which enables one end of the second liquid storage sink tank (405) to be communicated with one end of the second detection reaction sink tank (402) is formed in the detection liquid control valve plate (506).
6. The micro fluidic chip for detecting diseases based on urine as claimed in claim 5, wherein: one side of the second constant temperature reaction chip body (400) facing upwards is connected with a first control switch (501) and a second control switch (504), the upper end of the first control switch (501) is connected in the first upper connection sink tank, the upper end of the second control switch (504) is connected in the second upper connection sink tank, and the first control switch (501) and the second control switch (504) respectively control the sliding of the sample injection liquid control valve plate (503) and the detection liquid control valve plate (506).
7. The micro-fluidic chip for detecting diseases based on urine as claimed in any one of claims 1 to 3, wherein: one end down of second constant temperature reaction chip body (400) is equipped with the heating bath, be equipped with the heater strip in the heating bath, a plurality of first heat conduction liquid inlet (305) of having arranged on first constant temperature reaction chip body (300), second heat conduction liquid inlet (401) of a plurality of and first heat conduction liquid inlet (305) one-to-one have arranged on second constant temperature reaction chip body (400) in the heating bath outside.
8. The microfluidic chip for disease detection based on urine as claimed in claim 7, wherein: be equipped with heat-sensitive liquid apotheca 411 on the second constant temperature reaction chip body (400) of heating bath department, be connected with conducting rod (602) on the outer second constant temperature reaction chip body (400) of heating bath slidable, one side that the relative heating bath of conducting rod (602) set up is equipped with insulator spindle (601), heat-sensitive liquid apotheca 411 is filled in to the one end that conducting rod (602) were kept away from in insulator spindle (601), the cover is equipped with insulating reset spring (900) on insulator spindle (601), the one end of reset spring (900) is connected on second constant temperature reaction chip body (400), and the other end of reset spring (900) is connected in one side that conducting rod (602) set up the relative heating bath, all be connected with heating control conducting strip (700) on the second constant temperature reaction chip body (400) of conducting rod (602) both sides.
9. The micro fluidic chip for detecting diseases based on urine as claimed in claim 7, wherein: still all be connected with out liquid control conducting strip (800) on second constant temperature reaction chip body (400) of conducting rod (602) both sides, conducting rod (602) and play liquid control conducting strip (800) contact, when conducting rod (602) and play liquid control conducting strip (800) contact and break away from heating control conducting strip (700), the heater strip stop heating, and first control switch (501) and second control switch (504) control respectively advance a kind liquid control valve block (503) and detect liquid control valve block (506) and remove, make first feed liquor heavy groove and first detection reaction heavy groove (306) intercommunication, second feed liquor heavy groove and second detection reaction heavy groove (402) intercommunication.
10. The micro-fluidic chip for detecting diseases based on urine as claimed in any one of claims 1 to 3, wherein: a waste liquid port (301) is formed in the first constant-temperature reaction chip body (300) at the other end of the first detection reaction settling tank (306), a waste liquid settling tank (403) is formed in the second constant-temperature reaction chip body (400) at the other end of the second detection reaction settling tank (402), and the waste liquid port (301) and the waste liquid settling tank (403) form a waste liquid storage tank.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116920979A (en) * | 2023-09-12 | 2023-10-24 | 微纳动力(北京)科技有限责任公司 | Micro-fluidic chip fixture |
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