CN114643088B - Micro-droplet generation chip based on karman vortex street - Google Patents
Micro-droplet generation chip based on karman vortex street Download PDFInfo
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- CN114643088B CN114643088B CN202210246260.1A CN202210246260A CN114643088B CN 114643088 B CN114643088 B CN 114643088B CN 202210246260 A CN202210246260 A CN 202210246260A CN 114643088 B CN114643088 B CN 114643088B
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- vortex street
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- 239000012530 fluid Substances 0.000 claims abstract description 52
- 239000007788 liquid Substances 0.000 claims abstract description 41
- 238000000926 separation method Methods 0.000 claims abstract description 32
- 239000000758 substrate Substances 0.000 claims description 13
- 238000002347 injection Methods 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- 230000000737 periodic effect Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 description 8
- 238000010586 diagram Methods 0.000 description 7
- 230000015572 biosynthetic process Effects 0.000 description 4
- 238000005192 partition Methods 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 238000009776 industrial production Methods 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000004088 simulation Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 238000012695 Interfacial polymerization Methods 0.000 description 1
- 235000019483 Peanut oil Nutrition 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000009775 high-speed stirring Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000000312 peanut oil Substances 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 229920002545 silicone oil Polymers 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0463—Hydrodynamic forces, venturi nozzles
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micromachines (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
Abstract
The invention discloses a micro-droplet generating chip based on a karman vortex street, which is characterized in that a micro-flow channel is arranged in a body, the micro-flow channel is provided with a fluid separation part and a flow-around column, the fluid separation part is provided with an opening facing the downstream of the micro-flow channel, the fluid separation part separates the micro-flow channel into a middle flow area and side flow areas positioned at two sides of the middle flow area, the body is provided with a first liquid inlet and a second liquid inlet, the first liquid inlet is used for injecting liquid into the middle flow area, the second liquid inlet is used for injecting liquid into the side flow area, the flow-around column is arranged in the downstream direction of the fluid separation part and opposite to the opening of the fluid separation part, and a sufficient gap is reserved between the flow-around column and the fluid separation part, so that mixed incoming flows of the middle flow area and the side flow areas can form the karman vortex street through the flow-around column. The invention utilizes the vortex periodic shedding phenomenon of karman vortex street to achieve the aim of rapidly preparing micro-droplets, and has simple chip structure and long service life.
Description
Technical Field
The invention relates to a micro-droplet generation chip, in particular to a micro-droplet generation chip based on karman vortex street.
Background
The micro-droplets have huge application markets in the fields of colloidal particle synthesis, polymer material synthesis, biology, medical detection, food science and the like. Conventional methods of preparing microdroplets generally include: high-speed stirring, layer-by-layer deposition, membrane emulsification, interfacial polymerization, etc., which generally require multiple stages of processing and specific emulsion synthesis formulations, and do not allow precise regulation of the shell thickness of the complex-structured microdroplet or of the internal chamber structure and its components. In addition, the variability of shear forces used in conventional synthesis processes is high, resulting in large differences in size of the resulting microdroplets.
In order to overcome the defects and shortcomings of the conventional method for preparing micro-droplets, in recent years, many students in many fields are researching how to rapidly and accurately prepare micro-droplets by using the micro-fluidic chip technology, and many methods are fully verified in laboratories. The generation structure in the mainstream microfluidic chip comprises: t-shaped structures, flow focusing structures, and confocal structures. Compared with the traditional preparation method of the micro-droplets, the micro-droplets prepared by the micro-fluidic chip have 1 and less reagent consumption; 2. cross contamination is avoided; 3. the size of the generated micro-droplets is controllable, and the error is small. However, these structures still have the disadvantage that the generated micro-droplets are all located in the same horizontal line (see fig. 1 to 3), which results in the phenomenon that the micro-droplets are mutually fused to form new micro-droplets with larger size in the subsequent flow, which is unfavorable for the actual industrial production, so that the currently mainstream methods cannot completely meet the demands in the industrial production.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a micro-droplet generation chip based on karman vortex street, which reduces the fusion phenomenon of generated micro-droplets and enriches the method for preparing the micro-droplets by using the micro-fluidic chip so as to more meet the requirements of industrial production.
The technical scheme of the invention is as follows: the utility model provides a micro-droplet generates chip based on karman vortex street, includes the body, be equipped with the microchannel in the body, the microchannel is equipped with fluid separation portion and detouring post, fluid separation portion is equipped with the orientation the opening of the low reaches of microchannel, fluid separation portion makes the microchannel separates into middle part flow zone and is located the side flow zone of middle part flow zone both sides, the body is equipped with first inlet and second inlet, first inlet is used for to middle part flow zone injection liquid, the second inlet is used for to side flow zone injection liquid, detouring post sets up the low reaches direction of fluid separation portion and with the opening of fluid separation portion is relative, detouring post with leave sufficient clearance between the fluid separation portion makes the mixed incoming flow of middle part flow zone and side flow zone passes through detouring post becomes karman vortex street.
Further, the fluid partition portion extends from the bottom surface to the top surface of the micro flow channel, and the flow around column extends from the bottom surface to the top surface of the micro flow channel. The fluid separation part of the top-to-bottom can practically and reliably separate the fluids in the two flow areas, so that the two fluids form stable annular flow in the micro-flow channel between the opening of the fluid separation part and the flow-around column, and the flow-around column of the top-to-bottom can ensure that the annular flow completely flows through the side surface of the karman vortex street generating structure so as to be beneficial to the generation of the karman vortex street.
Further, in order to more easily generate karman vortex streets, the flow-around column is a cylinder.
Further, the cross section width of the flow around column is 1/4-1/2 of the width of the micro flow channel.
Further, the downstream end of the micro flow channel forms a micro droplet collection port on one side of the body.
Further, the body comprises an upper substrate and a lower bottom plate, a groove is formed in the lower bottom plate, and the groove forms the micro-channel when the upper substrate and the lower bottom plate are covered.
Further, the first liquid inlet and the second liquid inlet are arranged on the upper substrate.
The technical scheme provided by the invention has the advantages that:
1. Compared with the main flow method, the micro-liquid drops generated by the method are distributed on two sides of the central axis of the micro-flow channel in a random manner, the micro-liquid drops cannot be located on the same axis, and the situation that the micro-liquid drops are mutually fused to form micro-liquid drops with different sizes in the subsequent flow process is effectively avoided.
2. Compared with the traditional micro-droplet preparation process, the micro-droplet preparation method has the characteristics of rapid droplet generation, accurate and controllable droplet generation size, high reliability and long service life, and avoids external environment pollution.
Drawings
Fig. 1 is a schematic diagram of a T-shaped structure based micro-droplet generation structure.
Fig. 2 is a schematic diagram of a micro-droplet generation structure based on a flow focusing structure.
Fig. 3 is a schematic diagram of a micro-droplet generation structure based on a confocal structure.
Fig. 4 is a schematic structural diagram of a karman vortex street-based micro-droplet generation chip according to an embodiment of the present invention.
Fig. 5 is a schematic diagram of the structure of the lower plate of a karman vortex street-based micro-droplet generation chip.
Fig. 6 is a schematic view of the flow area of the micro flow channel of the karman vortex street based micro droplet generation chip.
FIG. 7 is a graph of a simulated fluid flow simulation of an embodiment of a karman vortex street based micro-droplet generation chip.
Detailed Description
The present application is further described below with reference to examples, which are to be construed as merely illustrative of the present application and not limiting of its scope, and various modifications to the equivalent arrangements of the present application will become apparent to those skilled in the art upon reading the present description, which are within the scope of the application as defined in the appended claims.
Referring to fig. 4,5 and 6, the micro-droplet generating chip based on karman vortex street in this embodiment includes a body composed of an upper substrate 1 and a lower substrate 2, a transverse groove 2-3 is formed on the lower substrate 2, one end of the groove 2-3 is closed, and the other end extends to the side surface of the lower substrate 2 to form a micro-droplet collecting port 2-4. The groove 2-3 is internally provided with a fluid separation part 2-1 and a flow-around column 2-2 at intervals, wherein the fluid separation part 2-1 is C-shaped, an opening faces the flow-around column 2-2, the flow-around column 2-2 is positioned at the downstream side of the fluid separation part 2-1, the cross section is round, in other words, the flow-around column 2-2 is a cylinder, the diameter of the cylinder can be 1/4-1/2 of the width of the groove 2-3, and the heights of the fluid separation part 2-1 and the flow-around column 2-2 are the same as the depth of the groove 2-3. The fluid separation part 2-1 and the flow around column 2-2 are both positioned on the central axis of the groove 2-3, and a gap is reserved between the two sides of the fluid separation part 2-1 and the side wall of the groove 2-3, so that a middle flow area A of fluid is formed inside the fluid separation part 2-1, and a side flow area B is formed outside the fluid separation part 2-1, namely, on the two sides of the middle flow area A. The upper top plate 1 is provided with a first liquid inlet 1-1 and a second liquid inlet 1-2, after the upper top plate 1 is covered on the lower bottom plate 2, the grooves 2-3 form micro-channels, and the top surfaces of the fluid separation part 2-1 and the flow around column 2-2 are tightly attached to the lower surface of the upper top plate 1. The first liquid inlet 1-1 is located opposite to the middle flow area A, and liquid enters the inner side of the fluid separation part 2-1 through the first liquid inlet 1-1, and forms liquid flow in the middle flow area A. The position of the second liquid inlet 1-2 corresponds to the groove 2-3 at the upstream end of the fluid partition 2-1, and liquid enters the outside of the fluid partition through the second liquid inlet 1-2 and forms liquid flow in the side flow area B. The fluid separator 2-1 itself should be of sufficient length to allow a stable flow of fluid in the central flow region a and the side flow regions B, while the fluid separator 2-1 is spaced from the bypass column 2-2 by a sufficient distance to allow the two liquids entering from the first inlet 1-1 and the second inlet 1-2 to form a stable annular flow in this region C.
In the embodiment, the calibers of the first liquid inlet 1-1 and the second liquid inlet 1-2 are respectively 0.35mm, the whole width of the micro flow channel is 5mm, and the length is 45mm; the thickness of the fluid partition 2-1 was 0.1mm, and the diameter of the circumferential flow column 2-2 was 1.6mm. When the device works, the first liquid inlet 1-1 and the second liquid inlet 1-2 are respectively connected with an external fluid pumping mechanism, two mutually-insoluble fluids are respectively introduced into the first liquid inlet 1-1 and the second liquid inlet 1-2, one group of common cooperation is that the first liquid inlet 1-1 is filled with silicone oil, and the second liquid inlet 1-2 is filled with water. Or water is introduced into the first liquid inlet 1-1, and peanut oil is introduced into the second liquid inlet 1-2. The fluid introduced from the first liquid inlet 1-1 and the second liquid inlet 1-2 flows into the micro flow channel, two mutually insoluble fluids are separated due to the action of the fluid separation part 2-1 on the front section part of the micro flow channel, and stable annular flow is formed in the micro flow channel between the fluid separation part 2-1 and the bypass column 2-2; when the annular flow flows through the flow-around column 2-2 at a proper speed, the karman vortex street phenomenon is generated, and the vortex of the karman vortex street periodically drops off to enable fluid flowing into the first liquid inlet to generate micro-droplets; the generated micro-droplets flow to the micro-droplet collecting port 2-4, and an external collecting device connected to the micro-droplet collecting port 2-4 may collect the generated micro-droplets. Referring to fig. 7, a numerical simulation is performed on the present embodiment by using COMSOL Multiphysics commercial simulation software, wherein the dark portion of the phase field diagram is the fluid introduced from the first inlet 1-1, and the bright portion is the fluid introduced from the second inlet 1-2. It can be seen that at 0.10 seconds a disturbance starts to occur at the flow-around column, at which point karman vortex street is being generated. When 0.15 seconds, vortex generated by karman vortex street can periodically and stably fall off, fluid at the dark part in the corresponding phase field diagram can periodically generate micro-droplets with the diameter of about 2mm, and the micro-droplets can be orderly distributed on two sides of the central axis of the micro-channel, so that the situation that the micro-droplets can be mutually fused to form micro-droplets with different sizes in the subsequent flowing process is effectively avoided.
Claims (7)
1. The utility model provides a micro-droplet generates chip based on karman vortex street, its characterized in that includes the body, be equipped with the microchannel in the body, the microchannel is equipped with fluid separation portion and round flow post, fluid separation portion is equipped with the orientation the opening of downstream of microchannel, fluid separation portion makes the microchannel separate into middle part flow zone and be located the side flow zone of middle part flow zone both sides, the body is equipped with first inlet and second inlet, first inlet is used for to middle part flow zone injection liquid, second inlet is used for to side flow zone injection liquid, round flow post sets up the downstream direction of fluid separation portion and with the opening of fluid separation portion is relative, round flow post with leave sufficient clearance between the fluid separation portion makes the mixed incoming flow of middle part flow zone and side flow zone passes through round flow post becomes karman vortex street.
2. The karman vortex street based micro-droplet generation chip of claim 1 wherein the fluid separator extends from the bottom surface to the top surface of the micro-fluidic channel and the flow around posts extend from the bottom surface to the top surface of the micro-fluidic channel.
3. The karman vortex street based micro-droplet generation chip of claim 1 wherein the flow around column is a cylinder.
4. The karman vortex street-based micro-droplet generation chip of claim 1, wherein the cross-sectional width of the flow-around column is 1/4-1/2 of the width of the micro-channel.
5. The karman vortex street based micro-droplet generation chip of claim 1 wherein the downstream end of the micro-channel forms a micro-droplet collection port on one side of the body.
6. The karman vortex street based micro-droplet generation chip according to any one of claims 1 to 5, wherein the body comprises an upper substrate and a lower substrate, the lower substrate is provided with a groove, and the groove forms the micro flow channel when the upper substrate and the lower substrate are covered.
7. The karman vortex street based micro-droplet generation chip of claim 6, wherein the first and second liquid inlets are disposed in the upper substrate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210246260.1A CN114643088B (en) | 2022-03-14 | 2022-03-14 | Micro-droplet generation chip based on karman vortex street |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210246260.1A CN114643088B (en) | 2022-03-14 | 2022-03-14 | Micro-droplet generation chip based on karman vortex street |
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| Publication Number | Publication Date |
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| CN114643088A CN114643088A (en) | 2022-06-21 |
| CN114643088B true CN114643088B (en) | 2024-04-19 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4151955A (en) * | 1977-10-25 | 1979-05-01 | Bowles Fluidics Corporation | Oscillating spray device |
| CN102059162A (en) * | 2002-06-28 | 2011-05-18 | 哈佛学院董事会 | Microfluidic device |
| CN108593956A (en) * | 2018-04-28 | 2018-09-28 | 中国空气动力研究与发展中心超高速空气动力研究所 | Micro- current meter of double mode and preparation method thereof |
| CN112007704A (en) * | 2020-07-08 | 2020-12-01 | 河海大学常州校区 | Micro-fluidic chip and method for sorting micro-nano particles by inertial turbulence |
| CN112547145A (en) * | 2020-11-19 | 2021-03-26 | 东南大学 | Rare cell rapid screening micro-fluidic device |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010110842A1 (en) * | 2009-03-25 | 2010-09-30 | Eastman Kodak Company | Droplet generator |
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- 2022-03-14 CN CN202210246260.1A patent/CN114643088B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4151955A (en) * | 1977-10-25 | 1979-05-01 | Bowles Fluidics Corporation | Oscillating spray device |
| CN102059162A (en) * | 2002-06-28 | 2011-05-18 | 哈佛学院董事会 | Microfluidic device |
| CN108593956A (en) * | 2018-04-28 | 2018-09-28 | 中国空气动力研究与发展中心超高速空气动力研究所 | Micro- current meter of double mode and preparation method thereof |
| CN112007704A (en) * | 2020-07-08 | 2020-12-01 | 河海大学常州校区 | Micro-fluidic chip and method for sorting micro-nano particles by inertial turbulence |
| CN112547145A (en) * | 2020-11-19 | 2021-03-26 | 东南大学 | Rare cell rapid screening micro-fluidic device |
Non-Patent Citations (2)
| Title |
|---|
| "Three-dimensional numerical simulation of the flow past a circular cylinder based on LES method";CHEN Hai-long;《Journal of Marine Science and Application》;第(2009) 8卷;第110-116页 * |
| 基于微通道构型的微流体流动控制研究;黄永光;刘世炳;陈涛;宋海英;;力学进展(01);第69-78页 * |
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