CN105413772A - Single/multiple-component droplet preparation device based on integrated micro-channels and control method of single/multiple-component droplet preparation device - Google Patents
Single/multiple-component droplet preparation device based on integrated micro-channels and control method of single/multiple-component droplet preparation device Download PDFInfo
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- CN105413772A CN105413772A CN201510939950.5A CN201510939950A CN105413772A CN 105413772 A CN105413772 A CN 105413772A CN 201510939950 A CN201510939950 A CN 201510939950A CN 105413772 A CN105413772 A CN 105413772A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0241—Drop counters; Drop formers
- B01L3/0244—Drop counters; Drop formers using pins
- B01L3/0251—Pin and ring type or pin in tube type dispenser
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0241—Drop counters; Drop formers
- B01L3/0268—Drop counters; Drop formers using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/061—Counting droplets
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
- B01L2300/0838—Capillaries
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/08—Regulating or influencing the flow resistance
- B01L2400/084—Passive control of flow resistance
- B01L2400/088—Passive control of flow resistance by specific surface properties
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Abstract
The invention discloses a single/multiple-component droplet preparation device based on integrated micro-channels and a control method of the single/multiple-component droplet preparation device. The device comprises a micro-channel plastic film, a dispersed phase inlet joint, a dispersed phase outlet joint, a continuous phase container, an injection pump and the like, wherein one end of the micro-channel plastic film is connected with the dispersed phase inlet joint, the other end is connected with the dispersed phase outlet joint, the dispersed phase inlet joint is connected with the injection pump, and the dispersed phase outlet joint is immersed in the container filled with a continuous phase. A dispersed phase is pushed by the injection pump to flow and the flow is controlled, so that dispersed phase droplets can be generated in the continuous phase container. Monodispersed droplets can be generated rapidly with a large flux, multiple-component droplets can be prepared, and the device is expected to be applied to the fields of drug preparation, chemical synthesis, biomedicine and the like.
Description
Technical field
The present invention relates to micro-fluidic field and chemical industry, biological pharmacy technical field, particularly relate to the list/many components drop preparation facilities based on integrated micro-channels and control method thereof.
Background technology
Drop detects the fields such as analysis and fine chemistry industry be widely used in drug particles preparation, medical science.The method preparing drop at present mainly contains micro-fluidic preparation method, spray-on process and capillary drop control method etc.Wherein, microfluidic methods utilizes some the specialized fluids flow mechanism in MCA to prepare drop, and its drop size uniformity produced is high, and controllability is strong, has significant superiority compared with additive method.
Microfluidic control is utilized mainly to comprise T-shaped passage method, fluid focus method and staged emulsion process (stepemulsification) etc. for the method for drop.Wherein, staged emulsion process has that monodispersity is good, energy ezpenditure is low and production frequency such as easily to adjust at the feature, and in actual production, advantage is particularly evident.But the production cycle being manufactured the microchannel apparatus needed for staged emulsification by processing methods such as photoetching is long, and cost is higher, is unfavorable for that actual industrialization is produced.In addition, preparation in enormous quantities is also a domestic and international technical barrier for a long time containing multi-component drop.Realize the efficient large flux of one-component or multicomponent drop and the production of low cost, significant for the association area such as chemical industry and bio-pharmaceuticals undoubtedly.
Summary of the invention
In view of above technical problem, the invention provides the list/many components drop preparation facilities based on integrated micro-channels and control method thereof, its objective is the large flux preparation realizing drop, simplify drop preparation technology, reduce cost prepared by drop, and the drop comprising multiple component can be produced, thus provide technical support for the development in the field such as chemical industry and bio-pharmaceuticals.
In order to arrive above-mentioned purpose, the technical solution adopted in the present invention is as follows: a kind of one-component drop preparation facilities based on integrated micro-channels, comprises discrete phase input system and drop formation system; Described discrete phase input system is connected with drop formation system; Described discrete phase input system comprises: syringe pump and discrete phase inlet attack; Described syringe pump is connected by pipeline with discrete phase inlet attack; Described discrete phase inlet attack comprises: adapter body, syringe needle and some capillaries; Described adapter body has inner chamber, and syringe needle inserts the top of adapter body and the intracavity inter-connection of adapter body, and syringe needle to be connected with adapter body by glue and to seal; Some capillaries insert the bottom of adapter body evenly distributedly, and are connected with the inner chamber of adapter body, and some capillaries to be connected with adapter body by glue and to seal;
Described drop formation system comprises: discrete phase outlet connection, microchannel plastic sheeting and continuous phase container; Described discrete phase outlet connection is made up of two panels clamping plate and two panels backing plate; Clip two panels backing plate between two panels clamping plate, between two panels clamping plate and two panels backing plate, form a cavity; Described microchannel plastic sheeting is inserted in described cavity, and bonding by glue; Described microchannel plastic sheeting contains series of parallel integrated micro-channels; Distance between described two panels clamping plate is h, and the bottom of microchannel plastic sheeting is l, then l >=3h to the distance of the bottom surface of discrete phase outlet connection; Described continuous phase container is equipped with continuous phase, and discrete phase outlet connection is immersed in continuous phase; The upper end grafting of described some capillaries and microchannel plastic sheeting.
Further, the contact angle between the material of described discrete phase outlet connection and discrete phase is greater than 90 degree.
Based on many components drop preparation facilities of integrated micro-channels, comprise drop formation system and N number of discrete phase input system; Wherein, N be more than or equal to 2 positive integer; Described some discrete phase input systems are connected with drop formation system; Described N number of discrete phase input system includes: syringe pump and discrete phase inlet attack; Described syringe pump is connected by pipeline with discrete phase inlet attack; Described discrete phase inlet attack comprises: adapter body, syringe needle and some capillaries; Described adapter body has inner chamber, and syringe needle inserts the top of adapter body and the intracavity inter-connection of adapter body, and syringe needle to be connected with adapter body by glue and to seal; Some capillaries insert the bottom of adapter body evenly distributedly, and are connected with the inner chamber of adapter body, and some capillaries to be connected with adapter body by glue and to seal;
Described drop formation system comprises: discrete phase outlet connection, microchannel plastic sheeting and continuous phase container; Described discrete phase outlet connection is made up of two panels clamping plate and two panels backing plate; Clip two panels backing plate between two panels clamping plate, between two panels clamping plate and two panels backing plate, form a cavity; Described microchannel plastic sheeting is inserted in described cavity, and bonding by glue; Described microchannel plastic sheeting contains series of parallel integrated micro-channels; Distance between described two panels clamping plate is h, and the bottom of microchannel plastic sheeting is l, then l >=3h to the distance of the bottom surface of discrete phase outlet connection; Described continuous phase container is equipped with continuous phase, and discrete phase outlet connection is immersed in continuous phase;
First capillary of the first discrete phase input system is connected with the first passage of microchannel plastic sheeting; First capillary of the second discrete phase input system is connected with the second channel of microchannel plastic sheeting; First capillary of the 3rd discrete phase input system is connected with the third channel of microchannel plastic sheeting; First capillary of N discrete phase input system is connected with the N channel of microchannel plastic sheeting; Second capillary of the first discrete phase input system is connected with the N+1 passage of microchannel plastic sheeting; Second capillary of the second discrete phase input system is connected with the N+2 passage of microchannel plastic sheeting; Second capillary of N discrete phase input system is connected with the 2N passage of microchannel plastic sheeting, the like.
In described discrete phase input system, the distance of adjacent two capillaries is greater than 5 (N+1) h; The passage connecting the jth capillary of the i-th discrete phase input system in the plastic sheeting of described microchannel is less than 5h with the distance of the passage of the jth capillary being connected the i-th+1 discrete phase input system; Wherein, i is the positive integer being less than N, and j is positive integer.
Further, the contact angle between the material of described discrete phase outlet connection and discrete phase is greater than 90 degree.
Based on a control method for the one-component drop preparation facilities of integrated micro-channels, specifically comprise the following steps:
In syringe pump, load discrete phase, in continuous phase container, load continuous phase, start syringe pump, syringe pump promotes discrete phase motion, according to the flow Q of required preparation speed determination syringe pump
1; When flowing exceed critical value, drop generation mechanism can be caused to change, drop size is increased rapidly; This transition process is by capillary number Ca
1=η U/ γ determines, wherein η is the viscosity of discrete phase, and U is discrete phase flow velocity, and γ is the surface tension between discrete phase and continuous phase; Because critical capillary number is between 0.04 to 0.06, for guarantee can not the mechanism sex reversal because flow is excessive, gets capillary number and be less than or equal to 0.04, be i.e. Ca
1≤ 0.04, then flow meets Q
1≤ 0.01n γ π d
2/ η, wherein, n is the actual number of channels used in the plastic sheeting of microchannel, and d is the diameter of microchannel in the plastic sheeting of microchannel; Step emulsification is there is in discrete phase at discrete phase outlet connection place, thus manufacture order component drop.
Based on a control method for many components drop preparation facilities of integrated micro-channels, specifically comprise the following steps:
In syringe pump, load discrete phase, in continuous phase container, load continuous phase, start all syringe pumps, syringe pump promotes discrete phase motion, and determine the flow Q of each syringe pump according to required preparation speed, the flow of each syringe pump is equal; When flowing exceed critical value, drop generation mechanism can be caused to change, drop size is increased rapidly; This transition process is by capillary number Ca
2=η
au
2/ γ
adetermine, wherein η
afor the mean value of the viscosity of different discrete phase, U
2for discrete phase flow velocity, γ
afor the capillary mean value between different discrete phase and continuous phase; Because critical capillary number is between 0.04 to 0.06, for guarantee can not the mechanism sex reversal because flow is excessive, gets capillary number and be less than or equal to 0.04, be i.e. Ca
2≤ 0.04, then flow meets Q≤0.01n γ
aπ d
2/ 1.4
n-1η
an, wherein, N be more than or equal to 2 positive integer; There is step emulsification at discrete phase outlet connection place in discrete phase, thus produces multicomponent drop.
When preparing one-component drop, select immiscible continuous phase and discrete phase as required.Appropriate surfactant can be added in continuous phase or discrete phase.The density of discrete phase is greater than the density of continuous phase.When preparing multicomponent drop, select continuous phase and plurality of discrete phase as required.Continuous phase and each discrete phase all do not dissolve each other, but dissolve each other between each discrete phase.Appropriate surfactant can be added in continuous phase or discrete phase.The density of each discrete phase is all greater than the density of continuous phase.
Compared with prior art, the invention has the beneficial effects as follows:
(1) compared to Traditional Industrialization drop production methods such as spray-on processes, the monodispersity of the drop that apparatus and method of the present invention prepare is good.
(2) compared to Microfluidic droplet preparation methods such as T-type structure and fluid focus structures, the method for the invention has structure and simple to operate, and the quantity of required pump is few, and the speed preparing drop significantly improves and is convenient to realize the advantages such as industry is integrated.
(3) the present invention can prepare the sophisticated functions drops such as many components drop under larger flux.
Accompanying drawing explanation
Fig. 1 is the device schematic diagram preparing one-component drop in the present invention;
Fig. 2 is discrete phase inlet attack sectional view in the present invention;
Fig. 3 be in the present invention discrete phase inlet attack A ?A to sectional view figure;
Fig. 4 is discrete phase outlet connection front view in the present invention;
Fig. 5 is discrete phase outlet connection top view in the present invention;
Fig. 6 is the installation diagram of microchannel plastic sheeting and discrete phase outlet connection in the present invention;
Fig. 7 is that in the embodiment of the present invention 1, one-component drop prepares schematic diagram;
Fig. 8 is the critical flow of mechanism transformation when in the embodiment of the present invention 2 prepared by single channel drop;
Fig. 9 is the device schematic diagram preparing many components drop in the present invention;
Figure 10 is that in the embodiment of the present invention 3, two component drop prepares schematic diagram;
Figure 11 is the critical flow of mechanism transformation when in the embodiment of the present invention 4 prepared by binary channels drop;
In figure, syringe pump 1, discrete phase inlet attack 2, adapter body 3, syringe needle 4, capillary 5, discrete phase outlet connection 6, microchannel plastic sheeting 7, continuous phase container 8, clamping plate 9, backing plate 10.
Detailed description of the invention
Below in conjunction with accompanying drawing and subordinate list embodiment, the present invention is described in further detail.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
As figure 1 ?shown in 6, a kind of one-component drop preparation facilities based on integrated micro-channels, comprises discrete phase input system and drop formation system; Described discrete phase input system is connected with drop formation system; Described discrete phase input system comprises: syringe pump 1 and discrete phase inlet attack 2; Described syringe pump 1 is connected by pipeline with discrete phase inlet attack 2; Described discrete phase inlet attack 2 comprises: adapter body 3, syringe needle 4 and some capillaries 5; Described adapter body 3 has inner chamber, and syringe needle 4 inserts the top of adapter body 3 and the intracavity inter-connection of adapter body 3, and syringe needle 4 to be connected with adapter body 3 by glue and to seal; Some capillaries 5 insert the bottom of adapter body 3 evenly distributedly, and are connected with the inner chamber of adapter body 3, and some capillaries 5 to be connected with adapter body 3 by glue and to seal;
Described drop formation system comprises: discrete phase outlet connection 6, microchannel plastic sheeting 7 and continuous phase container 8; Described discrete phase outlet connection 6 is made up of two panels clamping plate 9 and two panels backing plate 10; Clip two panels backing plate 10 between two panels clamping plate 9, between two panels clamping plate 9 and two panels backing plate 10, form a cavity; Described microchannel plastic sheeting 7 is inserted in described cavity, and bonding by glue; Described microchannel plastic sheeting contains series of parallel integrated micro-channels; Distance between described two panels clamping plate 9 is h, and the bottom of microchannel plastic sheeting 7 is l, then l >=3h to the distance of the bottom surface of discrete phase outlet connection 6; Described continuous phase container 8 is equipped with continuous phase, and discrete phase outlet connection 6 is immersed in continuous phase; The upper end grafting of described some capillaries 5 and microchannel plastic sheeting 7; Contact angle between the material of described discrete phase outlet connection 6 and discrete phase is greater than 90 degree.
As shown in Figure 9, a kind of drop of many components based on integrated micro-channels preparation facilities, comprises drop formation system and N number of discrete phase input system; Wherein, N be more than or equal to 2 positive integer; Described some discrete phase input systems are connected with drop formation system; Described N number of discrete phase input system includes: syringe pump 1 and discrete phase inlet attack 2; Described syringe pump 1 is connected by pipeline with discrete phase inlet attack 2; Described discrete phase inlet attack 2 comprises: adapter body 3, syringe needle 4 and some capillaries 5; Described adapter body 3 has inner chamber, and syringe needle 4 inserts the top of adapter body 3 and the intracavity inter-connection of adapter body 3, and syringe needle 4 to be connected with adapter body 3 by glue and to seal; Some capillaries 5 insert the bottom of adapter body 3 evenly distributedly, and are connected with the inner chamber of adapter body 3, and some capillaries 5 to be connected with adapter body 3 by glue and to seal;
Described drop formation system comprises: discrete phase outlet connection 6, microchannel plastic sheeting 7 and continuous phase container 8; Described discrete phase outlet connection 6 is made up of two panels clamping plate 9 and two panels backing plate 10; Clip two panels backing plate 10 between two panels clamping plate 9, between two panels clamping plate 9 and two panels backing plate 10, form a cavity; Described microchannel plastic sheeting 7 is inserted in described cavity, and bonding by glue; Described microchannel plastic sheeting contains series of parallel integrated micro-channels; Distance between described two panels clamping plate 9 is h, and the bottom of microchannel plastic sheeting 7 is l, then l >=3h to the distance of the bottom surface of discrete phase outlet connection 6; Described continuous phase container 8 is equipped with continuous phase, and discrete phase outlet connection 6 is immersed in continuous phase;
First capillary of the first discrete phase input system is connected with the first passage of microchannel plastic sheeting 7; First capillary of the second discrete phase input system is connected with the second channel of microchannel plastic sheeting 7; First capillary of the 3rd discrete phase input system is connected with the third channel of microchannel plastic sheeting 7; First capillary of N discrete phase input system is connected with the N channel of microchannel plastic sheeting 7; Second capillary of the first discrete phase input system is connected with the N+1 passage of microchannel plastic sheeting 7; Second capillary of the second discrete phase input system is connected with the N+2 passage of microchannel plastic sheeting 7; Second capillary of N discrete phase input system is connected with the 2N passage of microchannel plastic sheeting 7, the like.
In described discrete phase input system, the distance of adjacent two capillaries 5 is greater than 5 (N+1) h; The passage connecting the jth capillary of the i-th discrete phase input system in described microchannel plastic sheeting 7 is less than 5h with the distance of the passage of the jth capillary being connected the i-th+1 discrete phase input system; Wherein, i is the positive integer being less than N, and j is positive integer; Contact angle between the material of described discrete phase outlet connection 6 and discrete phase is greater than 90 degree.
Based on a control method for the one-component drop preparation facilities of integrated micro-channels, specifically comprise the following steps:
In syringe pump 1, load discrete phase, in continuous phase container 8, load continuous phase, start syringe pump, syringe pump promotes discrete phase motion, according to the flow Q of required preparation speed determination syringe pump
1; When flowing exceed critical value, drop generation mechanism can be caused to change, drop size is increased rapidly; This transition process is by capillary number Ca
1=η U/ γ determines, wherein η is the viscosity of discrete phase, and U is discrete phase flow velocity, and γ is the surface tension between discrete phase and continuous phase; Because critical capillary number is between 0.04 to 0.06, for guarantee can not the mechanism sex reversal because flow is excessive, gets capillary number and be less than or equal to 0.04, be i.e. Ca
1≤ 0.04, then flow meets Q
1≤ 0.01n γ π d
2/ η, wherein, n is the actual number of channels used in microchannel plastic sheeting 7, and d is the diameter of microchannel in microchannel plastic sheeting 7; Step emulsification is there is in discrete phase at discrete phase outlet connection 6 place, thus manufacture order component drop.
Based on a control method for many components drop preparation facilities of integrated micro-channels, specifically comprise the following steps:
In syringe pump 1, load discrete phase, in continuous phase container 8, load continuous phase, start all syringe pumps, syringe pump promotes discrete phase motion, and determine the flow Q of each syringe pump according to required preparation speed, the flow of each syringe pump is equal; When flowing exceed critical value, drop generation mechanism can be caused to change, drop size is increased rapidly; This transition process is by capillary number Ca
2=η
au
2/ γ
adetermine, wherein η
afor the mean value of the viscosity of different discrete phase, U
2for discrete phase flow velocity, γ
afor the capillary mean value between different discrete phase and continuous phase; Because critical capillary number is between 0.04 to 0.06, for guarantee can not the mechanism sex reversal because flow is excessive, gets capillary number and be less than or equal to 0.04, be i.e. Ca
2≤ 0.04, then flow meets Q≤0.01n γ
aπ d
2/ 1.4
n-1η
an, wherein, N be more than or equal to 2 positive integer; There is step emulsification at discrete phase outlet connection 6 place in discrete phase, thus produces multicomponent drop.
Device of the present invention is utilized to prepare the process of drop as follows: syringe pump 1 promotes discrete phase motion, and discrete phase enters discrete phase inlet attack 2, is then entered by capillary 5 in the microchannel of microchannel plastics plastic sheeting 7.There is step emulsification enter discrete phase outlet connection 6 from the microchannel microchannel plastic sheeting 7 after in discrete phase, its principle is: discrete phase expands by circle in outlet connection, and theoretical according to surface tension, the Laplace pressure of its inside is
wherein r
1for the radius of circle.Because h is much smaller than r
1, so
when discrete phase front end leave discrete phase outlet connection enter continuous phase time, discrete phase departs from the constraint of clamping plate 9, and start to carry out spherical expansion, its Laplace pressure is
wherein r
2for spherical radius.Work as r
2increase to a certain degree, P
1much larger than P
2, under this pressure differential, the discrete phase fluid in discrete phase outlet connection enters rapidly in continuous phase container, thus breaks to form drop.In multicomponent drop preparation process, the different discrete phases in adjacent N bar microchannel can merge after entering discrete phase outlet connection 6, and then form multicomponent drop by step emulsification.
The measuring method of liquid-drop diameter is as follows: take pictures to the process that drop produces, by the ratio of image processing software according to the size determination picture of physical unit, then go out the diameter of drop according to proportion measurement.
The preparation of embodiment 1 one-component aqueous phase droplets
(1) select continuous phase to be silicone oil, discrete phase is deionized water.In silicone oil, add the surfactant (DOW CORNING 749) of 5% mass fraction, and be contained in continuous phase container.Add a small amount of rhodamine b in deionized water, and load in syringe.
(2) preparation facilities of one-component drop is made according to said method.Distance between the two clamping plates of wherein discrete phase outlet connection is h=0.3mm, and microchannel outlet is 1.3mm with the distance on the downside of discrete phase outlet connection.Use two passes (the 3rd article with the 6th article) to prepare drop in the present embodiment, it is 5mL/h that this example arranges de-ionized water flow rate as required simultaneously.Open syringe pump and be transferred to described flow.
(3) namely deionized water starts to produce drop after arriving discrete phase outlet connection, and liquid-drop diameter is about 1.1mm, as shown in Figure 7.Aqueous phase droplets is collected in the bottom of silicone oil container.
The determination of mechanism transformation critical flow time prepared by embodiment 2 single channel drop
(1) select continuous phase to be that silicone oil adds 5% DOW CORNING 749, discrete phase is that deionized water adds a small amount of rhodamine b.The viscosity of water is 1.005mPas, and the surface tension between silicone oil and water is about 2mN/m, and channel internal diameter is 500 microns.
(2) preparation facilities of one-component drop is made according to said method.Distance between the two clamping plates of wherein discrete phase outlet connection is h=0.3mm, and microchannel outlet is 1.3mm with the distance on the downside of discrete phase outlet connection.
(3) use single channel to prepare drop in the present embodiment, flow is get a value every 4mL/hr between 2mL/hr to 66mL/hr, and gained drop size as shown in Figure 8.Visible, the mechanism sex reversal when flow increases to 58mL/hr, drop size increases rapidly.Capillary number Ca is now 0.041.The critical Ca number adopted in the present invention is 0.04, therefore in the scope of the present invention's suggestion, the transformation of drop Forming Mechanism can not occur.
The preparation of embodiment 3 pairs of component aqueous phase droplets
(1) select continuous phase to be silicone oil, in silicone oil, add the surfactant (DOW CORNING 749) of 5% mass fraction, and be contained in continuous phase container.Select the first discrete phase be with the addition of a small amount of rhodamine b deionized water and load in syringe.Select the second discrete phase be with the addition of a small amount of Nile blue deionized water and load in another syringe.
(2) preparation facilities of two component drop is made according to said method.Distance between the two clamping plates of wherein discrete phase outlet connection is h=0.3mm, and microchannel outlet is 1.3mm with the distance on the downside of discrete phase outlet connection.Use two passes to prepare two component drop in the present embodiment, two passes is logical respectively to be added the deionized water of rhodamine b and adds the deionized water of Nile blue.The spacing of two passes should be less than 5h=1.5mm, is 0.8mm herein.It is 1mL/h that this example arranges de-ionized water flow rate as required.Open two syringe pumps and be transferred to described flow.
Mutually merge after (3) two kinds of deionized waters arrive discrete phase outlet connection and start to produce two component drop, liquid-drop diameter is about 1.4mm, as shown in Figure 10.Two component aqueous phase droplets are collected in the bottom of silicone oil container.
The determination of mechanism transformation critical flow time prepared by embodiment 4 binary channels drop
(1) select continuous phase to be that to add 5% DOW CORNING 749, first discrete phase be that deionized water adds a small amount of rhodamine b to silicone oil, the second discrete phase is that deionized water adds a small amount of Nile blue.The viscosity of water is 1.005mPas, and the surface tension between silicone oil and two kinds of discrete phases is 2mN/m, and channel internal diameter is 500 microns.
(2) preparation facilities of one-component drop is made according to said method.Distance between the two clamping plates of wherein discrete phase outlet connection is h=0.3mm, and microchannel outlet is 1.3mm with the distance on the downside of discrete phase outlet connection.
(3) use two passes to prepare drop in the present embodiment, flow is get a value every 4mL/hr between 2mL/hr to 50mL/hr, and gained drop size as shown in figure 11.Visible, the mechanism sex reversal when flow increases to 42mL/hr, drop size increases rapidly.Capillary number Ca is now 0.03.The total flow of two kinds of discrete phases of advising in the present invention should be less than 0.01N γ
aπ d
2/ 1.4 η
a, calculating critical flow is 41mL/hr, so can not there is the transformation of drop Forming Mechanism in suggested range of the present invention.
Claims (6)
1. based on an one-component drop preparation facilities for integrated micro-channels, it is characterized in that, comprise discrete phase input system and drop formation system; Described discrete phase input system is connected with drop formation system; Described discrete phase input system comprises: syringe pump (1) and discrete phase inlet attack (2); Described syringe pump (1) is connected by pipeline with discrete phase inlet attack (2); Described discrete phase inlet attack (2) comprising: adapter body (3), syringe needle (4) and some capillaries (5) etc.; Described adapter body (3) has inner chamber, syringe needle (4) inserts the top of adapter body (3) and the intracavity inter-connection of adapter body (3), and syringe needle (4) is connected by glue with adapter body (3) and seals; Some capillaries (5) insert the bottom of adapter body (3) evenly distributedly, and be connected with the inner chamber of adapter body (3), some capillaries (5) are connected by glue with adapter body (3) and seal;
Described drop formation system comprises: discrete phase outlet connection (6), microchannel plastic sheeting (7) and continuous phase container (8); Described discrete phase outlet connection (6) is made up of two panels clamping plate (9) and two panels backing plate (10); Clip two panels backing plate (10) between two panels clamping plate (9), between two panels clamping plate (9) and two panels backing plate (10), form a cavity; Described microchannel plastic sheeting (7) is inserted in described cavity, and bonding by glue; Distance between described two panels clamping plate (9) is h, and the bottom of microchannel plastic sheeting (7) is l, then l >=3h to the distance of the bottom surface of discrete phase outlet connection (6); Described continuous phase container (8) is equipped with continuous phase, and discrete phase outlet connection (6) is immersed in continuous phase; The upper end grafting of described some capillaries (5) and microchannel plastic sheeting (7).
2. the one-component drop preparation facilities based on integrated micro-channels according to claim 1, is characterized in that, the contact angle between the material of described discrete phase outlet connection (6) and discrete phase is greater than 90 degree.
3. based on many components drop preparation facilities of integrated micro-channels, it is characterized in that, comprise drop formation system and N number of discrete phase input system; Wherein, N be more than or equal to 2 positive integer; Described some discrete phase input systems are connected with drop formation system; Described N number of discrete phase input system includes: syringe pump (1) and discrete phase inlet attack (2); Described syringe pump (1) is connected by pipeline with discrete phase inlet attack (2); Described discrete phase inlet attack (2) comprising: adapter body (3), syringe needle (4) and some capillaries (5); Described adapter body (3) has inner chamber, syringe needle (4) inserts the top of adapter body (3) and the intracavity inter-connection of adapter body (3), and syringe needle (4) is connected by glue with adapter body (3) and seals; Some capillaries (5) insert the bottom of adapter body (3) evenly distributedly, and be connected with the inner chamber of adapter body (3), some capillaries (5) are connected by glue with adapter body (3) and seal;
Described drop formation system comprises: discrete phase outlet connection (6), microchannel plastic sheeting (7) and continuous phase container (8); Described discrete phase outlet connection (6) is made up of two panels clamping plate (9) and two panels backing plate (10); Clip two panels backing plate (10) between two panels clamping plate (9), between two panels clamping plate (9) and two panels backing plate (10), form a cavity; Described microchannel plastic sheeting (7) is inserted in described cavity, and bonding by glue; Distance between described two panels clamping plate (9) is h, and the bottom of microchannel plastic sheeting (7) is l, then l >=3h to the distance of the bottom surface of discrete phase outlet connection (6); Described continuous phase container (8) is equipped with continuous phase, and discrete phase outlet connection (6) is immersed in continuous phase;
First capillary of the first discrete phase input system is connected with the first passage of microchannel plastic sheeting (7); First capillary of the second discrete phase input system is connected with the second channel of microchannel plastic sheeting (7); First capillary of the 3rd discrete phase input system is connected with the third channel of microchannel plastic sheeting (7); First capillary of N discrete phase input system is connected with the N channel of microchannel plastic sheeting (7); Second capillary of the first discrete phase input system is connected with the N+1 passage of microchannel plastic sheeting (7); Second capillary of the second discrete phase input system is connected with the N+2 passage of microchannel plastic sheeting (7); Second capillary of N discrete phase input system is connected with the 2N passage of microchannel plastic sheeting (7), the like.
In described discrete phase input system, the distance of adjacent two capillaries (5) is greater than 5 (N+1) h; The passage connecting the jth capillary of the i-th discrete phase input system in described microchannel plastic sheeting (7) is less than 5h with the distance of the passage of the jth capillary being connected the i-th+1 discrete phase input system; Wherein, i is the positive integer being less than N, and j is positive integer.
4. the drop of the many components based on integrated micro-channels preparation facilities according to claim 3, is characterized in that, the contact angle between the material of described discrete phase outlet connection (6) and discrete phase is greater than 90 degree.
5., based on a control method for the one-component drop preparation facilities of integrated micro-channels, it is characterized in that, specifically comprise the following steps:
In syringe pump (1), load discrete phase, in continuous phase container (8), load continuous phase, start syringe pump, syringe pump promotes discrete phase motion, according to the flow Q of required preparation speed determination syringe pump
1; When flowing exceed critical value, drop generation mechanism can be caused to change, drop size is increased rapidly; This transition process is by capillary number Ca
1=η U/ γ determines, wherein η is the viscosity of discrete phase, and U is discrete phase flow velocity, and γ is the surface tension between discrete phase and continuous phase; Because critical capillary number is between 0.04 to 0.06, for guarantee can not the mechanism sex reversal because flow is excessive, gets capillary number and be less than or equal to 0.04, be i.e. Ca
1≤ 0.04, then flow meets Q
1≤ 0.01n γ π d
2/ η, wherein, n is the actual number of channels used in microchannel plastic sheeting (7), and d is the diameter of microchannel in microchannel plastic sheeting (7); Step emulsification is there is in discrete phase at discrete phase outlet connection (6) place, thus manufacture order component drop.
6., based on a control method for many components drop preparation facilities of integrated micro-channels, it is characterized in that, specifically comprise the following steps:
Discrete phase is loaded in syringe pump (1), in continuous phase container (8), load continuous phase, start all syringe pumps, syringe pump promotes discrete phase motion, determine the flow Q of each syringe pump according to required preparation speed, the flow of each syringe pump is equal; When flowing exceed critical value, drop generation mechanism can be caused to change, drop size is increased rapidly; This transition process is by capillary number Ca
2=η
au
2/ γ
adetermine, wherein η
afor the mean value of the viscosity of different discrete phase, U
2for discrete phase flow velocity, γ
afor the capillary mean value between different discrete phase and continuous phase; Because critical capillary number is between 0.04 to 0.06, for guarantee can not the mechanism sex reversal because flow is excessive, gets capillary number and be less than or equal to 0.04, be i.e. Ca
2≤ 0.04, then flow meets Q≤0.01n γ
aπ d
2/ 1.4
n-1η
an, wherein, N be more than or equal to 2 positive integer; There is step emulsification at discrete phase outlet connection (6) place in discrete phase, thus produces multicomponent drop.
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