CN112892630A - Multi-material liquid drop generation system based on coaxial flow focusing structure - Google Patents
Multi-material liquid drop generation system based on coaxial flow focusing structure Download PDFInfo
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Abstract
The invention belongs to the technical field of micro-fluidic, and discloses a multi-material liquid drop generating system based on a coaxial flow focusing structure; the device comprises a liquid drop generating device and a liquid drop collecting device, wherein the liquid drop generating device comprises a discrete phase input device, the output end of the discrete phase input device is connected with a reaction unit, and the discrete phase input device is connected with the input end of the reaction unit; the input end of the reaction unit is also connected with a continuous phase input device, the output end of the reaction unit is connected with a liquid drop output device, and the liquid drop output device is communicated with a liquid drop collecting device through a photocuring system; the invention can generate liquid drops of various materials at the same time, and the photocuring system can solidify the liquid drops into various shapes.
Description
Technical Field
The invention relates to the technical field of micro-fluidic, in particular to a multi-material liquid drop generating system based on a coaxial flow focusing structure.
Background
The micro-droplets have increasingly wide application in the fields of biochemistry, medicine, materials and the like, and from the development process of droplet preparation technology, the preparation of the micro-droplets is subject to the traditional emulsification method, the micro-fluidic chip method and the new off-chip preparation method appearing in recent years.
For the preparation of monodisperse liquid drops at present, the passive preparation technology of the micro-fluid liquid drops, such as the micro-fluid chip technology, the capillary glass tube technology, the stainless steel needle tube flowing focusing technology and the like, has simple structure, easy operation and control and high speed of producing the liquid drops, but the generation frequency of the liquid drops prepared by the methods is unpredictable and difficult to accurately control; the active preparation technology of the micro-fluid droplets can accurately control the generation of uniform micro-droplets, but the introduction of external equipment is required, and a control system is complex.
The design of the coaxial flow focusing device can prepare micro-droplets with smaller diameter with higher production rate, so far, the coaxial flow focusing structure is mostly made of glass capillary or multi-layer SU-8 photoresist or PDMS (polydimethylsiloxane), the existing droplet generation device has low droplet generation efficiency, and a device capable of rapidly and simultaneously generating droplets of a plurality of different materials is lacked.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides the multi-material liquid droplet generation system based on the coaxial flow focusing structure, which has the advantages of simple and stable technology, good monodispersity, wide material applicability and capability of simultaneously generating a plurality of different material droplets.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a multi-material liquid droplet generation system based on a coaxial flow focusing structure comprises a droplet generation device and a droplet collection device, wherein the droplet generation device comprises a discrete phase input device, the output end of the discrete phase input device is connected with a reaction unit capable of generating droplets, the discrete phase input device is connected with the input end of the reaction unit, the input end of the reaction unit is further connected with a continuous phase input device, and the output end of the reaction unit is connected with a droplet output device; the liquid drop output device is communicated with the liquid drop collecting device through a light curing system capable of curing the liquid drops.
Furthermore, the continuous phase input device comprises a continuous phase conveying pipe which is vertically arranged, the continuous phase conveying pipe is of a cylindrical structure, the top end of the continuous phase conveying pipe is provided with an interface connected with a pipeline, and the bottom end of the continuous phase conveying pipe is connected with a continuous phase shunt pipe; the continuous phase shunt tube is of a hollow tubular structure which is vertically arranged, the central axis of the continuous phase shunt tube is used as a rotating shaft, a plurality of conveying tubes matched with the reaction units are uniformly distributed at the bottom end of the continuous phase shunt tube, and the conveying tubes are hollow rectangular tubes.
Further, the reaction unit comprises an upper reaction cavity and a lower reaction cavity, wherein the upper reaction cavity is vertically arranged, and the lower reaction cavity is matched with the upper reaction cavity; the upper reaction cavity comprises an outer side capillary tube, the outer side capillary tube is of a cylindrical structure with an opening at the bottom end, a hollow inner side capillary tube which is coaxial with the outer side capillary tube is arranged in the outer side capillary tube, and the bottom end of the inner side capillary tube penetrates through the opening of the outer side capillary tube and extends into the lower reaction cavity; the top end of the upper reaction cavity is provided with a discrete phase interface matched with the discrete phase input device, the top end of the upper reaction cavity is also provided with a rectangular boss, and the boss is provided with a rectangular continuous phase interface matched with the conveying pipe; the discrete phase interface is communicated with the inner cavity of the inner capillary, and the continuous phase interface is communicated with the inner cavity of the outer capillary; the lower reaction cavity is of a cylindrical structure with an opening at the top end, the bottom end of the lower reaction cavity is provided with a spray head, and the spray head is a cylindrical boss communicated with the lower reaction cavity.
Furthermore, the light curing system comprises a light curing chip and a DMD digital micro-mirror, wherein two ends of the light curing chip are respectively connected with the liquid drop output device and the liquid drop collecting device, and the DMD digital micro-mirror is connected with a computer module.
Furthermore, the light curing chip is of a rectangular plate-shaped structure with the length of 5cm, the width of 5cm and the height of 300 microns, a plurality of grooves with the depth of 150 microns are uniformly distributed on the light curing chip, the two ends of each groove are respectively provided with an input end and an output end, the width of each input end and the width of each output end are both 200 microns, the length of each input end and the length of each output end are both 1cm, and the width of the middle part of each groove is 500 microns.
Further, the liquid drop output device comprises a plurality of liquid drop output pipes which are coaxially arranged with the reaction unit, each liquid drop output pipe is of a hollow tubular structure, a bowl-shaped interface matched with the spray head is arranged at the top end of each liquid drop output pipe, and a pipe sleeve is arranged at the bottom end of each liquid drop output pipe.
Further, the discrete phase input device comprises a plurality of discrete phase conveying pipes coaxially arranged with the reaction unit, and the discrete phase conveying pipes are matched with discrete phase interfaces at the top end of the upper reaction cavity.
Furthermore, the liquid drop generating device also comprises a protective shell, the protective shell comprises an upper protective shell and a lower protective shell which are spliced, and the reaction unit and the continuous phase shunt tube are arranged in the protective shell; the continuous phase conveying pipe and the discrete phase conveying pipe extend out of the upper protective shell, and the liquid drop output pipe extends out of the lower protective shell.
Further, a multi-material liquid droplet generation method based on a coaxial flow focusing structure comprises the following steps:
s1: inputting the continuous phase into the inner capillary of the reaction unit through a continuous phase delivery pipe by using a syringe pump, and delivering the discrete phases of different materials into a gap between the outer capillary and the inner capillary of the reaction unit through a plurality of discrete phase delivery pipes;
s2: the continuous phase and the discrete phase generate liquid drops at the pipe orifice of the capillary pipe at the inner side due to the shearing action of the two-phase interface, and the liquid drops are conveyed to a liquid drop output pipe through a nozzle of the reaction unit;
s3: the liquid drops are conveyed into the photocuring chip through a pipeline, and the liquid drops in the photocuring chip are cured into different shapes by illumination with different projection shapes emitted by the DMD digital micro-mirror;
s4: and the solidified liquid drops are conveyed to a liquid drop collecting device through a pipeline to complete the collection of the liquid drops.
Furthermore, the diameter of the inner capillary is 215-243 μm, the diameter of the outer capillary is 440-460 μm, the diameter of the nozzle is 140-160 μm, and the diameters of the continuous phase transport pipe and the discrete phase transport pipe are 240-260 μm.
Compared with the prior art, the invention has the beneficial effects that:
(1) the device is provided with a plurality of discrete phase conveying pipes, each discrete phase conveying pipe is connected with the reaction unit, and continuous phases of different materials can be conveyed to the capillary tube on the inner side of the reaction unit through the discrete phase conveying pipes; the continuous phase is shunted to different reaction units through the continuous phase shunt tube, the continuous phase and the discrete phase generate liquid drops due to the shearing action of two-phase interfaces at the pipe orifice of the capillary at the inner side, and the liquid drops are transported to the light curing system through a pipeline to be cured and then collected, so that the aim of simultaneously generating liquid drops of different materials is fulfilled, and the generation efficiency of the liquid drops is greatly improved.
(2) The light curing system comprises a light curing chip, the light curing chip is provided with a groove with two thin ends and a thick middle part, and the two ends of the groove are respectively connected with the liquid drop output device and the liquid drop collecting device; the liquid drops enter the groove on the photocuring chip, and the moving speed of the liquid drops is reduced due to the fact that the width of the groove is increased, so that the liquid drops are more conveniently cured; the light curing system also comprises a DMD digital micromirror connected with the computer module, the DMD digital micromirror emits light with different projection shapes to cure the liquid drops, the liquid drops can be cured into different shapes, and the DMD digital micromirror can meet the requirement that light with different wavelengths is irradiated to cure liquid drops of respective materials in different channels, so that the effect that the whole system generates liquid drops with multiple materials and multiple shapes at the same time is achieved.
(3) One end of the liquid drop output pipe is provided with a bowl-shaped interface, so that liquid drops discharged from the nozzle can be better received; because the diameter of the liquid drop output pipe is very small, one end of the liquid drop output pipe is provided with the pipe sleeve, the connection of the pipeline is more convenient, and the operation is convenient.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the system of the present invention;
FIG. 2 is an exploded view of a droplet generator according to the present invention;
FIG. 3 is a schematic diagram of the internal structure of a droplet generation apparatus without a protective shell;
FIG. 4 is a schematic three-dimensional structure of an upper reaction chamber;
FIG. 5 is a cross-sectional view of FIG. 4 taken along a vertical plane;
FIG. 6 is a schematic three-dimensional structure of a lower reaction chamber;
FIG. 7 is a cross-sectional view of FIG. 6 taken along a vertical plane;
FIG. 8 is a half sectional view of the upper and lower reaction chambers when they are mated;
FIG. 9 is a schematic three-dimensional structure of a continuous phase shunt tube;
FIG. 10 is a cross-sectional view of FIG. 9 taken along the vertical plane;
FIG. 11 is a schematic three-dimensional view of a droplet output tube in cooperation with a tube sleeve;
FIG. 12 is a cross-sectional view of FIG. 11 taken along the vertical plane;
fig. 13 is a schematic three-dimensional structure diagram of a light-cured chip.
In the figure: continuous phase conveyer pipe 1, discrete phase conveyer pipe 2, upper protective housing 3, lower protective housing 4, liquid drop output tube 5, pipe box 6, photocuring chip 7, liquid drop collecting pipe 8, support 9, computer module 10, DMD digital micromirror 11, light 12, continuous phase shunt 13, last reaction chamber 14, lower reaction chamber 15, discrete phase interface 16, continuous phase interface 17, shower nozzle 18, conveyer pipe 19.
Detailed Description
In order to make the technical solutions of the present invention better understood, the present invention is further explained below with reference to the accompanying drawings.
Referring to fig. 1-13, a multi-material liquid droplet generation system based on a coaxial flow focusing structure comprises a droplet generation device, wherein the droplet generation device comprises a continuous phase input device, the continuous phase input device comprises a vertically arranged continuous phase conveying pipe 1, the top end of the continuous phase conveying pipe 1 is an input end, and the bottom end of the continuous phase conveying pipe 1 is an output end; the diameter of the continuous phase conveying pipe 1 is a hollow cylindrical structure of 250 micrometers, the top end of the continuous phase conveying pipe 1 is provided with a cylindrical interface with a larger diameter, the bottom end of the continuous phase conveying pipe 1 is connected with a continuous phase shunt pipe 13, the continuous phase shunt pipe 13 is a hollow tubular structure which is vertically arranged, the central axis of the continuous phase shunt pipe 13 is used as a rotating shaft, six conveying pipes 19 are uniformly distributed at the bottom end of the continuous phase shunt pipe 13, the size of an internal channel of each conveying pipe 19 is 70 micrometers in width and 40 micrometers in height, the six conveying pipes 19 are annularly distributed, each conveying pipe 19 is a hollow rectangular pipe, and the output end of each conveying pipe 19 is connected with a reaction unit.
The reaction unit comprises an upper reaction cavity 14 and a lower reaction cavity 15, wherein the upper reaction cavity 14 is vertically arranged, and the lower reaction cavity is matched with the upper reaction cavity 14; the upper reaction cavity 14 comprises an outer side capillary tube, the outer side capillary tube is a cylindrical structure with an opening at the bottom end, the diameter of the outer side capillary tube is 450 micrometers, a hollow inner side capillary tube which is coaxial with the outer side capillary tube is arranged in the outer side capillary tube, the diameter of the inner side capillary tube is 229 micrometers, and the bottom end of the inner side capillary tube penetrates through the opening of the outer side capillary tube and penetrates into the lower reaction cavity 15; the top end of the upper reaction cavity 14 is provided with a discrete phase interface 16, the discrete phase interface 16 is connected with a discrete phase input device, the discrete phase input device is a vertically arranged discrete phase conveying pipe 2, the diameter of the discrete phase conveying pipe 2 is 250 micrometers, one end of the discrete phase conveying pipe 2 is connected with the discrete phase interface 16 of the reaction unit, and the other end of the discrete phase conveying pipe is provided with a cylindrical interface matched with the pipeline; the top end of the upper reaction cavity 14 is also provided with a rectangular boss, and the boss is provided with a rectangular continuous phase interface 17 matched with the conveying pipe 19; the discrete phase interface 16 is communicated with the inner cavity of the inner capillary, and the continuous phase interface 17 is communicated with the inner cavity of the outer capillary; the lower reaction chamber 15 is a cylindrical structure with an opening at the top end, the bottom end of the lower reaction chamber 15 is provided with a spray head 18, the diameter of the spray head 18 is 150 microns, the spray head 18 is a cylindrical boss communicated with the lower reaction chamber 15, and the spray head 18 is connected with a liquid drop output device.
The liquid drop output device comprises a liquid drop output pipe 5 which is coaxial with the reaction unit, the liquid drop output pipe 5 is of a hollow tubular structure, the top end of the liquid drop output pipe 5 is provided with a bowl-shaped interface matched with the spray head 18, and the bottom end of the liquid drop output pipe 5 is provided with a pipe sleeve 6 which is conveniently connected with a pipeline; the liquid drop output pipe 5 is connected with a liquid drop collecting device through a pipeline, the liquid drop collecting device comprises a support 9, six different liquid drop collecting pipes 8 connected with the liquid drop output pipe 5 are arranged on the support 9, and labels for recording material names are arranged on the liquid drop collecting pipes 8.
The droplet generating device further comprises a protective shell, and the upper protective shell 3 and the lower protective shell 4 are formed by 3D printing of printer resin materials (the printing resin materials are numbered as M09-HARZ-HTM140M, and the manufacturer is envisionTEC). The protective shell comprises an upper protective shell 3 and a lower protective shell 4 which are spliced, the upper protective shell 3 and the lower protective shell 4 are respectively provided with a pin and a jack, and the upper protective shell 3 and the lower protective shell 4 are spliced; the reaction unit and the continuous phase shunt pipe 13 are arranged in the protective shell; continuous phase conveyer pipe 1, discrete phase conveyer pipe 2 stretch out the setting of protective housing 3, and protective housing 4 setting is down stretched out to liquid droplet output tube 5.
A photocuring system for curing the liquid drops is further arranged between the liquid drop output device and the liquid drop collecting device, the photocuring system comprises a photocuring chip 7, two ends of the photocuring chip 7 are respectively connected with the liquid drop output device and the liquid drop collecting device, the photocuring chip 7 is made of PDMS (polydimethylsiloxane) material and is of a rectangular plate-shaped structure with the length of 5cm, the width of 5cm and the height of 300 mu m, a plurality of grooves with the depth of 150 mu m are uniformly distributed on the photocuring chip 7, two ends of each groove are respectively an input end and an output end, the width of each input end and the width of each output end are both 200 micrometers, the length of each groove is 1cm, and the width of the middle; the light curing system further comprises a DMD digital micromirror 11, and the DMD digital micromirror 11 is connected with a computer module 10.
A multi-material liquid drop generation method based on a coaxial flow focusing structure comprises the following steps:
s1: inputting a continuous phase into an inner capillary of the reaction unit through a continuous phase delivery pipe 1 by using a syringe pump, and delivering discrete phases of different materials into a gap between the outer capillary and the inner capillary of the reaction unit through different discrete phase delivery pipes 2;
s2: the continuous phase and the discrete phase generate liquid drops at the pipe orifice of the capillary pipe at the inner side due to the shearing action of the two-phase interface, and the liquid drops are conveyed to a liquid drop output pipe 5 through a nozzle of the reaction unit;
s3: the liquid drops are conveyed into the photocuring chip 7 through a pipeline, and the liquid drops in the photocuring chip 7 are solidified into different shapes by light rays 12 with different projection shapes emitted by the DMD digital micromirror 11;
s4: and the solidified liquid drops are conveyed to a liquid drop collecting device through a pipeline to complete the collection of the liquid drops.
According to the device provided by the application, a plurality of discrete phase conveying pipes 2 are arranged, each discrete phase conveying pipe 2 is connected with a reaction unit, and continuous phases of different materials can be conveyed to capillaries on the inner side of the reaction unit through the discrete phase conveying pipes 2; the continuous phase is shunted to different reaction units through a continuous phase shunt pipe 13, liquid drops are generated at the mouth of a capillary pipe at the inner sides of the continuous phase and the discrete phase due to the shearing action of two-phase interfaces, the liquid drops are transported to a light curing chip 7 of a light curing system through a pipeline, the liquid drops are cured through light rays 12 with different projection shapes emitted by a DMD digital micro-mirror 11 and then collected, so that the purpose of simultaneously generating liquid drops with different materials is achieved, the generation efficiency of the liquid drops is greatly improved, the micro-liquid drops with good cell compatibility can be produced, the micro-liquid drops are applied to tissue engineering, the coaxial flow focusing structure solves the problem that the micro-fluidic chip technology and the like have strong dependence on the operation of professionals to a certain extent, and the micro-fluidic chip technology and the like are expected to be widely applied to the fields of biochemistry and the like.
Example 2
A multi-material liquid drop generating system based on a coaxial flow focusing structure is different from the embodiment 1 only in that the diameters of a continuous phase conveying pipe 1 and a discrete phase conveying pipe 2 are 240 micrometers, the cross section size of an inner channel of a conveying pipe 19 on a vertical surface is 60 micrometers in length and 30 micrometers in width, the diameter of an inner capillary is 215 micrometers, the diameter of an outer capillary is 440 micrometers, and the diameter of a spray head 18 is 140 micrometers.
Example 3
A multi-material liquid drop generating system based on a coaxial flow focusing structure is different from the embodiment 1 only in that the diameters of a continuous phase conveying pipe 1 and a discrete phase conveying pipe 2 are 260 micrometers, the cross section of an internal channel of a conveying pipe 19 in a vertical plane is 80 micrometers long and 50 micrometers wide, the diameter of an inner capillary is 243 micrometers, the diameter of an outer capillary is 460 micrometers, and the diameter of a spray head 18 is 160 micrometers.
Example 4
The utility model provides a many materials liquid droplet produces system based on coaxial current focus structure, the difference with embodiment 1 only lies in, and discrete looks conveyer pipe 2, reaction unit, droplet output tube 5 all are equipped with two, are equipped with two recesses on the photocuring chip 7, can produce the droplet of two kinds of different materials simultaneously.
Example 5
The utility model provides a many materials liquid droplet produces system based on coaxial current focus structure, the difference with embodiment 1 only lies in that, and discrete phase conveyer pipe 2, reaction unit, droplet output tube 5 all are equipped with four, are equipped with four recesses on the photocuring chip 7, can produce the droplet of four different materials simultaneously.
Example 6
The utility model provides a many materials liquid droplet produces system based on coaxial current focus structure, the difference with embodiment 1 only lies in that discrete phase conveyer pipe 2, reaction unit, droplet output tube 5 all are equipped with seven, are equipped with seven recesses on the photocuring chip 7, can produce the droplet of seven different materials simultaneously.
Claims (10)
1. A multi-material liquid droplet generation system based on a coaxial flow focusing structure comprises a droplet generation device and a droplet collection device, and is characterized in that the droplet generation device comprises a discrete phase input device, the output end of the discrete phase input device is connected with a reaction unit, and the discrete phase input device is connected with the input end of the reaction unit; the input end of the reaction unit is also connected with a continuous phase input device, the output end of the reaction unit is connected with a liquid drop output device, and the liquid drop output device is communicated with a liquid drop collecting device through a light curing system.
2. The multi-material liquid droplet generation system based on the coaxial flow focusing structure as claimed in claim 1, wherein the continuous phase input device comprises a vertically arranged continuous phase conveying pipe, the continuous phase conveying pipe is of a cylindrical structure, the top end of the continuous phase conveying pipe is provided with an interface connected with a pipeline, and the bottom end of the continuous phase conveying pipe is connected with a continuous phase shunt pipe; the continuous phase shunt tube is of a hollow tubular structure which is vertically arranged, the central axis of the continuous phase shunt tube is used as a rotating shaft, a plurality of conveying tubes matched with the reaction units are uniformly distributed at the bottom end of the continuous phase shunt tube, and the conveying tubes are hollow rectangular tubes.
3. The coaxial flow focusing structure-based multi-material droplet generation system of claim 2, wherein the reaction unit comprises an upper reaction chamber and a lower reaction chamber, wherein the upper reaction chamber is vertically arranged, and the lower reaction chamber is matched with the upper reaction chamber; the upper reaction cavity comprises an outer capillary tube, the outer capillary tube is of a cylindrical structure with an opening at the bottom end, an inner capillary tube which is coaxial with the outer capillary tube is arranged in the outer capillary tube, and the inner capillary tube penetrates through the opening of the outer capillary tube and extends into the lower reaction cavity; the top end of the upper reaction cavity is provided with a discrete phase interface matched with the discrete phase input device, the top end of the upper reaction cavity is also provided with a rectangular continuous phase interface matched with the conveying pipe, the discrete phase interface is communicated with the inner cavity of the inner capillary, and the continuous phase interface is communicated with the inner cavity of the outer capillary; the lower reaction cavity is of a cylindrical structure with an opening at the top end, and a spray head is arranged at the bottom end of the lower reaction cavity.
4. The coaxial flow focusing structure-based multi-material droplet generation system of claim 3, wherein the light curing system comprises a light curing chip, and two ends of the light curing chip are respectively connected with the droplet output device and the droplet collection device; the light curing system also comprises a DMD digital micromirror used in cooperation with the light curing chip, and the DMD digital micromirror is connected with a computer module.
5. The coaxial flow focusing structure-based multi-material droplet generation system of claim 4, wherein the photo-curing chip is a rectangular plate-shaped structure, a plurality of grooves are uniformly distributed on the photo-curing chip, and the grooves are rectangular grooves with two thin ends and a thick middle.
6. The system for generating droplets of a multi-material liquid based on a coaxial flow focusing structure of claim 5, wherein the droplet output device comprises a plurality of droplet output tubes coaxially arranged with the reaction unit, the droplet output tubes are hollow tubular structures, the top ends of the droplet output tubes are provided with bowl-shaped interfaces matched with the nozzles, and the bottom ends of the droplet output tubes are provided with pipe sleeves.
7. The coaxial flow focusing structure-based multi-material droplet generation system of claim 6, wherein the discrete phase input device comprises a plurality of discrete phase delivery pipes coaxially disposed with the reaction unit, the discrete phase delivery pipes being engaged with the discrete phase interfaces at the top end of the upper reaction chamber.
8. The coaxial flow focusing structure-based multi-material droplet generation system of claim 7, wherein the droplet generation device further comprises a protective housing, the reaction unit is disposed in the protective housing, and the continuous phase transport pipe, the discrete phase transport pipe and the droplet output pipe all extend out of the protective housing.
9. A multi-material liquid drop generation method based on a coaxial flow focusing structure comprises the following steps:
s1: the injection pump inputs the continuous phase into the inner capillary of the reaction unit through the continuous phase conveying pipe, and the discrete phases of different materials are conveyed into a gap between the outer capillary and the inner capillary of the reaction unit through the plurality of discrete phase conveying pipes;
s2: the continuous phase and the discrete phase generate liquid drops at the pipe orifice of the capillary pipe at the inner side due to the shearing action of the two-phase interface, and the liquid drops are conveyed to a liquid drop output pipe through a nozzle of the reaction unit;
s3: the liquid drops are conveyed into the photocuring chip through a pipeline, and the liquid drops in the photocuring chip are cured into different shapes by illumination with different projection shapes emitted by the DMD digital micro-mirror;
s4: and the solidified liquid drops are conveyed to a liquid drop collecting device through a pipeline to complete the collection of the liquid drops.
10. The method as claimed in claim 9, wherein the diameter of the inner capillary is 215-243 μm, the diameter of the outer capillary is 440-460 μm, the diameter of the nozzle is 140-160 μm, and the diameters of the continuous phase transport pipe and the discrete phase transport pipe are 240-260 μm.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114210378A (en) * | 2021-11-22 | 2022-03-22 | 广东省科学院健康医学研究所 | Liquid drop generating device based on microporous capillary and preparation method thereof |
| CN114225988A (en) * | 2021-11-30 | 2022-03-25 | 广东省科学院健康医学研究所 | Bidirectional configuration microfluidic droplet generation device and preparation method thereof |
| CN115350734A (en) * | 2022-08-08 | 2022-11-18 | 广东省科学院生物与医学工程研究所 | Laminated multi-channel liquid drop micro-fluidic chip and preparation method thereof |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090131543A1 (en) * | 2005-03-04 | 2009-05-21 | Weitz David A | Method and Apparatus for Forming Multiple Emulsions |
| CN101507909A (en) * | 2009-02-25 | 2009-08-19 | 中国科学院过程工程研究所 | Molecular engram microspheres preparation method using micro-fluidic reactor |
| CN101695646A (en) * | 2009-10-28 | 2010-04-21 | 中国海洋大学 | Device and method for preparing gel microspheres with uniform grain sizes |
| CN105413772A (en) * | 2015-12-15 | 2016-03-23 | 浙江大学 | Single/multiple-component droplet preparation device based on integrated micro-channels and control method of single/multiple-component droplet preparation device |
| WO2017060876A1 (en) * | 2015-10-09 | 2017-04-13 | King Abdullah University Of Science And Technology | Microfluidic droplet generator with controlled break-up mechanism |
| CN106582549A (en) * | 2016-11-10 | 2017-04-26 | 广西大学 | Preparing method for monodisperse lignin microspheres |
| CN107890785A (en) * | 2017-10-26 | 2018-04-10 | 浙江大学 | A kind of the electro' asion preparation facilities and control method of more component drops |
| CN109289950A (en) * | 2018-10-19 | 2019-02-01 | 扬州大学 | A kind of preparation facilities and method of porous microsphere |
| CN110038656A (en) * | 2019-05-31 | 2019-07-23 | 中国科学技术大学 | A kind of aqueous two-phase system and its drop formation module for emulsification |
| CN111229070A (en) * | 2020-02-28 | 2020-06-05 | 广东工业大学 | Device for generating multiple emulsion drops in large batch |
| CN111229069A (en) * | 2020-02-28 | 2020-06-05 | 广东工业大学 | Efficient liquid drop generating device |
-
2021
- 2021-03-16 CN CN202110280588.0A patent/CN112892630B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090131543A1 (en) * | 2005-03-04 | 2009-05-21 | Weitz David A | Method and Apparatus for Forming Multiple Emulsions |
| CN101507909A (en) * | 2009-02-25 | 2009-08-19 | 中国科学院过程工程研究所 | Molecular engram microspheres preparation method using micro-fluidic reactor |
| CN101695646A (en) * | 2009-10-28 | 2010-04-21 | 中国海洋大学 | Device and method for preparing gel microspheres with uniform grain sizes |
| WO2017060876A1 (en) * | 2015-10-09 | 2017-04-13 | King Abdullah University Of Science And Technology | Microfluidic droplet generator with controlled break-up mechanism |
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