CN114636700A - Closed-loop protection observation platform for pathological section gene identification - Google Patents
Closed-loop protection observation platform for pathological section gene identification Download PDFInfo
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- CN114636700A CN114636700A CN202210534423.6A CN202210534423A CN114636700A CN 114636700 A CN114636700 A CN 114636700A CN 202210534423 A CN202210534423 A CN 202210534423A CN 114636700 A CN114636700 A CN 114636700A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
- G01N2001/065—Drive details
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Abstract
The invention relates to a pathological section gene identification closed-loop protection observation platform which comprises a shell frame and a freezing box arranged at the upper end of the shell frame, wherein an observation area is arranged at the right side of the shell frame, a control panel is arranged on an inclined plane at the right side of the shell frame, the pathological section gene identification closed-loop protection observation platform also comprises a closed-loop section component, the closed-loop section component penetrates through cavities arranged in the shell frame and the freezing box, the closed-loop section component is hermetically connected with the shell frame and the freezing box, the closed-loop section component is electrically connected with the control panel, the closed-loop section component is used for freezing sections of pathological tissues in a closed-loop state, and sliced pathological tissue slices are transferred to the lower part of the observation area for optical microscopic observation. The invention can realize the section sampling of the pathological tissue in the closed loop state, the pathological tissue can not contact with the outside air, and the accuracy of the subsequent detection of the pathological tissue is ensured.
Description
Technical Field
The application relates to the technical field of medical pathological detection, in particular to a closed-loop protection observation platform for pathological section gene identification.
Background
Pathological section detection requires a series of technical treatments of cut pathological tissue organs, including fixation, material taking, dehydration, wax immersion, embedding, slicing, staining and the like, and generally takes 24 hours to complete the whole process of section making, and then a pathologist observes the pathological tissue organs by using a microscope to make a diagnosis. This examination method is called conventional paraffin section, and is the most commonly used method in pathological examination. However, this method usually takes 3 days from when the surgeon cuts the lesion tissue to when the pathological diagnosis is made. Sometimes, a surgeon wants to know the nature of a lesion immediately during an operation to determine the operation range in time and to perform corresponding treatment, so that a pathologist is required to perform pathological diagnosis during the operation, and at the moment, the pathological diagnosis must be performed by fast slicing, and fast freezing of the slices is the most widely used method.
In current pathological tissue section equipment, like the chinese patent of publication No. CN215825459U, it discloses a pathological sample freezing section device, and is concrete, and it includes the section case, section case below is equipped with freezing case, section roof portion is equipped with observes the glass board, it is equipped with status display screen to observe glass board one side, status display screen one side is equipped with control panel, the side that the section case leaned on observing the glass board is equipped with manual section rotation handle, section device fixed plate left side is equipped with the seat of placing of waiting to cut the sample of two equipartitions, it is equipped with blade holder translation turning handle to wait to cut sample placing seat right side, blade holder translation turning handle rotates and connects on the blade holder, be equipped with the blade fixing base on the blade holder, blade fixing base upper portion is equipped with the blade fixed slot, the permanent cold box both sides are equipped with the mechanism that looses wet. Compared with the prior art, the method has the advantages that: the freezing box and the slicing box are placed together, so that the time of the sample at normal temperature is shortened, and the moisture dispersing device is arranged in the freezing box, so that the storage effect of the sample is better.
In the prior art, the pathological tissue is also sliced in a freezing manner, but on the first hand, in the prior art, the pathological tissue is sliced by a blade, the sliced tissue cannot be sliced under a closed-loop condition, and when the slice is sliced, the tissue slice needs to be removed from the equipment, so that the tissue slice can be in contact with the outside air, and the preparation effect of the tissue slice is affected; in the second aspect, because pathological tissue takes place the phenomenon of deformation after freezing easily, can not flatten the pathological tissue surface usually, when slicing into slices the pathological tissue after freezing, incomplete phenomenon may appear in sliced pathological tissue, is unfavorable for pathological tissue's follow-up accurate inspection, and required section thickness is different when different tissue inspection simultaneously, and regulation section thickness that can not be accurate is based on this, on the technique of current pathological section device, there is the space that can improve still.
Disclosure of Invention
In order to realize accurate section processing of pathological tissues in a sealed environment, the application provides a closed-loop protection observation platform for pathological section gene identification.
The utility model provides a closed-loop protection observation platform for pathological section gene identification adopts following technical scheme:
the utility model provides a pathological section gene identification closed loop protection observation platform, include the casing frame and set up in the freezer of casing frame upper end, the casing frame right side is provided with the observation area, install control panel on the inclined plane on casing frame right side, still include closed loop section subassembly, closed loop section subassembly runs through the setting in the inside cavity of casing frame and freezer, closed loop section subassembly and casing frame and freezer sealing connection, closed loop section subassembly is connected with control panel electricity, closed loop section subassembly is used for the frozen section of the pathological tissue under the closed loop attitude, the pathological tissue thin slice after the section is transferred and is used for the microscopic observation of optics below the observation area.
Through adopting the above technical scheme, in the in-service use process, place behind the pathological tissue stripping piece inside closed loop section subassembly, place the slide glass in the assigned position simultaneously, the freezer carries out high-efficient freezing to the pathological tissue stripping piece, closed loop section subassembly cuts into slices the pathological tissue after freezing, make arranging at the slide glass middle part that the section is accurate, transfer the slide glass below the cover glass in observation district in order to form the tissue preforming again, carry out the analysis to the tissue preforming through optical microscope, the section sampling in-process of pathological tissue is in the closed loop form, pathological tissue can not contact with the outside air, guarantee the accuracy of the follow-up detection of pathological tissue.
Preferably, demountable installation has the board of placing on the observation area, places the board and is the rectangle structure, evenly is provided with negative pressure groove on placing the board, and the negative pressure cooperatees with the coverslip, evenly is provided with the negative pressure hole on the negative pressure groove, and the negative pressure hole is connected with negative-pressure air fan, and negative-pressure air fan installs on placing the board lateral wall, and negative pressure downthehole portion is provided with the filtration sponge.
Through adopting above-mentioned technical scheme, when the cover glass was placed inside the negative pressure inslot, the negative pressure fan can adsorb fixedly can enough realize the location to the cover glass through the negative pressure hole to the cover glass, can guarantee the leakproofness that the cover glass is connected again, avoids the outside air to get into.
Preferably, the closed-loop slicing assembly comprises a slicing unit and a transferring unit, the slicing unit is located in a cavity in the middle of the freezing box, the transferring unit is located in a cavity in the middle of the shell frame, the slicing unit is detachably inserted into the freezing box, and the upper end of the transferring unit is matched with the slicing unit.
Through adopting above-mentioned technical scheme, the section unit can be cut into slices to the pathological tissue after freezing, and on the slide glass was arranged in to the pathological tissue thin slice after the section is accurate, transfer the unit and transfer the cover glass below of observation area with the slide glass in order to form the tissue preforming, be convenient for pathological tissue's accuracy, quick detection.
Preferably, the section unit includes the grafting frame, removes frame, section mechanism, bearing plate, feed mechanism, collects frame and support bracket, the grafting frame is U type structure, and the grafting frame is provided with removes the frame, removes frame and grafting frame joint cooperation, and the terminal surface upper end is provided with section mechanism before removing the frame, removes a mid-mounting and has even bearing plate, and the bearing plate rear end is connected with section mechanism through feed mechanism, removes the frame preceding terminal surface lower extreme and is provided with and collects the frame, removes the support bracket that the frame lower extreme was installed and is used for placing the slide glass.
Through adopting above-mentioned technical scheme, the pathological tissue after will cutting loads in the bearing plate middle part, place the slide glass on bearing the bracket again, after freezing the pathological tissue at the freezer, at first the slicer constructs to advance the cutting to the pathological tissue in advance, in order to reach the purpose of leveling the pathological tissue surface, the pathological tissue after cutting in advance drops inside collecting the frame, when the slicer constructs the motion and carries out the section to the official pathological tissue, collecting the frame inwards withdraws and resets, slicer constructs through feed mechanism promotion pathological tissue slow feed, guarantee the even section of pathological tissue, adsorb the section through the mode of negative pressure, when slicer constructs the downstream and arrives the undermost end, slicer constructs the pathological tissue after will cutting and transports the slide glass of horizontal placement on the mode of blowback.
Preferably, the slicing mechanism comprises an electric slider, a moving plate, a connecting frame and cutting blades, sliding grooves are symmetrically formed in the front end face of the moving frame, the electric slider is connected to the inside of each sliding groove in a sliding fit mode, the moving plate is arranged on the electric slider and is of an I-shaped structure, the connecting frame is installed between the front ends of the moving plates, and the cutting blades are evenly arranged on the connecting frame.
Through adopting above-mentioned technical scheme, electronic slider can drive cutting blade up-and-down motion through the movable plate, can realize carrying out sliced function to the pathological tissue surface through cutting blade's reciprocating motion, does benefit to the follow-up inspection of pathological tissue.
Preferably, the cutting blades are obliquely arranged, orifices are uniformly arranged on the cutting blades, and the orifices are connected with the negative pressure fan.
Through adopting above-mentioned technical scheme, when cutting blade precuts to pathological tissue, negative pressure fan is out of work, when cutting blade carried out the accuracy section to pathological tissue, negative pressure fan passes through the drill way and adsorbs the thin slice after the section, when cutting blade moved the bottom, negative pressure fan stop work, and the accurate dropping of the thin slice of slope is on the slide.
Preferably, the support plate comprises a fan grid, a fixed frame, a feeding plate and a gear carrier, the fan grid is uniformly arranged in a groove at the front end of the movable frame, the fan grid is made of heat conducting materials, the fixed frame is arranged in the middle of the fan grid, the feeding plate is arranged in the middle of the fixed frame in a sliding mode, inserting needles are uniformly arranged on the front end face of the feeding plate, the gear carrier is arranged at the rear end of the fixed frame through a bearing, a screw rod is connected to the middle of the gear carrier in a threaded fit mode, the end of the screw rod is connected with the feeding plate through the bearing, and the gear carrier is meshed with the feeding mechanism.
By adopting the technical scheme, the fan grating adopts the through hole type design, the freezing efficiency of pathological tissues is improved, the time is saved, when the slicing mechanism reciprocates, the slicing mechanism can drive the gear carrier to intermittently rotate, and the gear carrier can further drive the feeding plate to push materials forwards through the screw rod, so that the pathological tissues can be accurately fed.
Preferably, feed mechanism includes pivot, connecting gear, transmitting gear and latch, the pivot is installed through the bearing to the removal frame is inside, evenly is provided with connecting gear in the pivot, and connecting gear meshes with the carrier mutually, is provided with transmitting gear between the adjacent connecting gear, and transmitting gear meshes with the latch mutually, and the latch is installed in movable plate medial surface rectangular channel a1, and latch quantity is a plurality of, and the latch is connected with the rectangular channel through the round pin axle, is provided with the torsional spring on the round pin axle.
Through adopting above-mentioned technical scheme, when the slicing mechanism upward movement, the slicing mechanism drives running gear through the latch and rotates, and running gear and then drives pathological tissue through gear complex mode and feed, and when the slicing mechanism downward movement, the latch does not mesh with running gear mutually.
Preferably, the transferring unit comprises a vertical moving module, a transverse moving module, a transferring assembly and a guide frame, the vertical moving module is symmetrically arranged on the right side of the middle of the shell frame, the electromagnet matched with the slicing unit is arranged on the vertical moving module, the transverse moving module is symmetrically arranged on the middle of the shell frame, the transferring assembly is installed at the upper end of the transverse moving module, the lower end of the transferring assembly is tightly attached to the guide frame, the guide frame is installed on the inner wall of the shell frame, and the guide frame is provided with an inclined smooth surface.
Through adopting above-mentioned technical scheme, vertical removal module drives and removes a downstream for support bracket downstream to transfer the subassembly upper end, and the slide glass on the support bracket subassembly contacts with transferring the subassembly, and later, the subassembly left motion is transferred in the horizontal movement module drive, and the leading truck can drive and transfer subassembly upward movement, makes slide glass and the coverslip laminating under the closed loop condition on transferring the subassembly, avoids the pathological tissue thin slice between slide glass and the coverslip to contact in the air circumstance.
Preferably, transfer the subassembly and include crossbeam board, guide arm, expanding spring and transfer the board, install the crossbeam board between the lateral shifting module, evenly be provided with the through-hole on the crossbeam board, it is provided with the guide arm to slide in the through-hole, and the guide arm lower extreme is the smooth surface, is provided with expanding spring on the guide arm, installs the diaphragm between the guide arm upper end, and the board is transferred to evenly installing in diaphragm upper end, transfers the board and evenly is provided with the suction hole for tilt up structure, transfer the board surface.
Through adopting above-mentioned technical scheme, the leading truck passes through the guide arm and drives and transfer the board upward movement for the slide glass on transferring the board can be accurate cooperate with the coverslip, and expanding spring plays the effect that resets, does benefit to and transfers the board and resets.
In summary, the present application includes at least one of the following beneficial technical effects:
in order to accurately place the cut tissue slices on the glass slide, the cutting blade is obliquely arranged, orifices are uniformly arranged on the cutting blade and are connected with a negative pressure fan, when the cutting blade precuts pathological tissues, the negative pressure fan does not work, when the cutting blade formally slices the pathological tissues, the negative pressure fan adsorbs the sliced slices through the orifices, when the cutting blade moves to the lowest end, the negative pressure fan stops working, and the oblique slices accurately drop on the glass slide;
in order to prevent the frozen pathological tissues from falling off from the inside of the fixed frame, the front end of the feeding plate is provided with an inserting needle, the end part of the inserting needle is of a convex structure, when the pathological tissues are not frozen, the pathological tissues can be accurately filled in gaps among the inserting needles, when the pathological tissues are frozen, the convex structure can effectively prevent the tissues from falling off, and after the frozen tissues are completely thawed, the pathological tissues can be effectively removed;
in order to ensure the accuracy of the pathological tissue after slicing, the frozen pathological tissue needs to be precut to flatten the surface of the pathological tissue, so that the cut film is positioned in the middle of the pathological tissue, and the precut pathological tissue falls into the collecting rack;
in order to slice pathological tissues with different thicknesses, the number of the clamping teeth is increased or reduced to increase or reduce the feeding amount of the pathological tissues driven by the feeding mechanism, so that the function of slicing the pathological tissues with different thicknesses can be realized.
Drawings
The invention is further illustrated with reference to the following figures and examples.
Fig. 1 is a schematic perspective view of the present application.
Fig. 2 is a schematic sectional structure of the present application.
Fig. 3 is a schematic view of the structure of the placing board of the present application.
Fig. 4 is a schematic perspective view of a slicing unit according to the present application.
Fig. 5 is a schematic cross-sectional structure of the slicing unit of the present application.
Fig. 6 is a schematic cross-sectional structure diagram of the slicing unit of the present application.
Fig. 7 is a schematic cross-sectional view of a support plate according to the present application.
Fig. 8 is a schematic view of the structure of the feed plate of the present application.
Fig. 9 is a schematic structural view between the moving plate and the latch according to the present application.
Fig. 10 is a schematic view of the structure between the support bracket and the take-off plate according to the present invention.
Description of reference numerals: 1. a housing frame; 11. placing the plate; 2. a freezer; 3. a closed-loop slicing assembly; 31. a slicing unit; 311. a plug-in frame; 312. a movable frame; 313. a slicing mechanism; 3131. an electric slider; 3132. moving the plate; 3133. a connecting frame; 3134. a cutting blade; 314. a support plate; 3141. a fan grill; 3142. a fixed frame; 3143. a feed plate; 3144. a gear carrier; 315. a feed mechanism; 3151. a rotating shaft; 3152. a connecting gear; 3153. a rotating gear; 3154. clamping teeth; 316. a collecting rack; 317. a support bracket; 32. a transfer unit; 321. a vertical movement module; 322. a lateral movement module; 323. a transfer assembly; 3231. a beam plate; 3232. a guide bar; 3233. a tension spring; 3234. a transfer plate; 324. a guide frame.
Detailed Description
The present application is described in further detail below with reference to figures 1-10.
The embodiment of the application discloses a closed-loop protection observation platform for pathological section gene identification, which can accurately realize the function of sectioning tissues of pathological components under the closed-loop sealing condition.
The first embodiment is as follows:
referring to fig. 1, a closed-loop protection observation desk for pathological section gene identification disclosed in this embodiment includes a housing frame 1 and a freezing box 2 disposed on the upper end of the housing frame 1, an observation area is disposed on the right side of the housing frame 1, a control panel is mounted on an inclined plane on the right side of the housing frame 1, and the closed-loop protection observation desk further includes a closed-loop slicing assembly 3, the closed-loop slicing assembly 3 penetrates through cavities disposed inside the housing frame 1 and the freezing box 2, the closed-loop slicing assembly 3 is hermetically connected with the housing frame 1 and the freezing box 2, the closed-loop slicing assembly 3 is electrically connected with the control panel, the closed-loop slicing assembly 3 is used for freezing a slice of a pathological tissue in a closed-loop state, and a sliced pathological tissue slice is transferred below the observation area for optical microscopic observation.
Observation area is last demountable installation to place board 11, places board 11 and is the rectangle structure, evenly is provided with the negative pressure groove on placing board 11, and the negative pressure cooperatees with the cover glass, evenly is provided with the negative pressure hole on the negative pressure groove, and the negative pressure hole is connected with negative-pressure air fan, and negative-pressure air fan installs on placing the board lateral wall, and negative pressure downthehole portion is provided with the filtration sponge.
The closed-loop slicing assembly 3 comprises a slicing unit 31 and a transferring unit 32, the slicing unit 31 is located in a cavity in the middle of the freezing box 2, the transferring unit 32 is located in a cavity in the middle of the shell frame 1, the slicing unit 31 is detachably inserted into the freezing box 2, and the upper end of the transferring unit 32 is matched with the slicing unit 31.
In the in-service use process, place inside section unit 31 behind the pathological tissue dice, place slide glass in the assigned position simultaneously, freezer 2 carries out high-efficient freezing to the pathological tissue dice, section unit 31 slices the pathological tissue after freezing, make arranging at the slide glass middle part that the section is accurate, transfer unit 32 again with slide glass transfer to the coverslip below of observation district in order to form the tissue preforming, carry out the analysis to the tissue preforming through optical microscope, the section sampling process of pathological tissue is in the closed loop state, pathological tissue can not contact with the outside air, guarantee the accuracy of the follow-up detection of pathological tissue.
The inside refrigeration module that is provided with of freezer 2, the refrigeration module has the function of refrigeration and defrosting, finishes when the pathological tissue freezing, defrosts pathological tissue through the defrosting mode, prevents to appear the phenomenon of ice crystal on the tissue.
It should be noted that the refrigeration temperature of the refrigeration module is adjustable, the refrigeration module is connected with the control panel, and different refrigeration temperatures, such as thyroid, spleen, kidney, muscle and other tissues, can be adjusted to the left or right according to different pathological tissues, the adipose-containing tissues should be adjusted to the left or right, and the adipose-rich tissues should be adjusted to the left or right.
Meanwhile, the movable sliding rail is arranged at the left end of the shell frame 1, and the optical microscope is installed on the sliding rail, so that the optical microscope can accurately move, and tissues at different positions of an observation area can be observed conveniently.
Example two:
referring to fig. 3 to 9, on the basis of the first embodiment, in order to realize accurate slicing processing of pathological tissues, the second embodiment is provided with a corresponding slicing unit 31.
Referring to fig. 4, in the second embodiment, the slicing unit 31 includes an insertion frame 311, a moving frame 312, a slicing mechanism 313, a supporting plate 314, a feeding mechanism 315, a collecting frame 316 and a supporting bracket 317, the insertion frame 311 is U-shaped, the insertion frame 311 is provided with the moving frame 312, the moving frame 312 is in snap fit with the insertion frame 311, the slicing mechanism 313 is provided at the upper end of the front end face of the moving frame 312, the uniform supporting plate 314 is installed at the middle part of the moving frame 312, the rear end of the supporting plate 314 is connected with the slicing mechanism 313 through the feeding mechanism 315, the collecting frame 316 is provided at the lower end of the front end face of the moving frame 312, and the supporting bracket 317 for placing a slide glass is installed at the lower end of the moving frame 312.
In the actual use process, the pathological tissues after being cut into pieces are filled in the middle of the supporting plate 314, then the glass slide is placed on the supporting bracket 317, after the freezing box 2 freezes the pathological tissues, firstly, the slicing mechanism 313 performs precutting on the pathological tissues to achieve the purpose of flattening the surfaces of the pathological tissues, the precutting pathological tissues fall into the collecting frame 316, when the slicing mechanism 313 moves to slice the pathological tissues, the collecting frame 316 retracts inwards to reset, the slicing mechanism 313 pushes the pathological tissues to slowly feed through the feeding mechanism 315 to ensure the uniform slicing of the pathological tissues, the slices are adsorbed through a negative pressure mode, and when the slicing mechanism 313 moves downwards to the lowest end, the slicing mechanism 313 conveys the sliced pathological tissues to the glass slide horizontally placed in a back blowing mode.
Referring to fig. 5-6, in order to ensure the accuracy of pathological tissue slice cutting, in this embodiment, a corresponding slicing mechanism 313 is provided, the slicing mechanism 313 includes an electric slider 3131, a moving plate 3132, a connecting frame 3133 and a cutting blade 3134, sliding grooves are symmetrically provided on the front end surface of the moving frame 312, the electric slider 3131 is connected in the sliding grooves in a sliding fit manner, the moving plate 3132 is provided on the electric slider 3131, the moving plate 3132 is an i-shaped structure, the connecting frame 3133 is installed between the front ends of the moving plate 3132, and the cutting blade 3134 is uniformly provided on the connecting frame 3133.
In the actual working process, the electric slider 3131 can drive the cutting blade 3134 to move up and down, and the movement of the cutting blade 3134 can realize the function of slicing the surface of the pathological tissue, so that the subsequent examination of the pathological tissue is facilitated.
In order to ensure the accuracy of the pathological tissue after slicing, the frozen pathological tissue needs to be precut to flatten the surface of the pathological tissue, so that the cut film is in the middle of the pathological tissue, and the precut pathological tissue falls into the collecting frame 316.
It should be noted that the lower end of the front end face of the movable frame 312 is provided with a collecting frame 316, the collecting frame 316 can collect pathological tissues, the collecting frame 316 is connected with a control panel through an electric push rod, when the tissues are precut, the electric push rod drives the collecting frame 316 to be pushed out, the collecting frame 316 is inclined, and the tissues inside the collecting frame 316 can be favorably dropped in a collecting tank.
In order to accurately place the sliced tissue slices on the slide glass, in this embodiment, the cutting blade 3134 is tilted, and the cutting blade 3134 is uniformly provided with an opening connected to a negative pressure fan, the negative pressure fan does not work when the cutting blade 3134 precuts the pathological tissue, the negative pressure fan sucks the sliced tissue slices through the opening when the cutting blade 3134 formally slices the pathological tissue, and the negative pressure fan stops working when the cutting blade 3134 moves to the lowest end, so that the tilted tissue slices accurately fall on the slide glass.
In order to improve the freezing efficiency of pathological tissues, in the embodiment, the support plate 314 is provided, the support plate 314 includes a fan grating 3141, a fixing frame 3142, a feeding plate 3143 and a gear rack 3144, the fan grating 3141 is uniformly installed in a groove at the front end of the moving frame 312, the fan grating 3141 is made of a heat conducting material, the fan grating 3141 can rapidly carry heat inside the pathological tissues in the freezing process to improve the cooling efficiency of the pathological tissues, the fixing frame 3142 is installed at the middle part of the fan grating 3141, the feeding plate 3143 is slidably installed at the middle part of the fixing frame 3142, plug-in pins are uniformly arranged on the front end surface of the feeding plate 3143, the gear rack 3144 is installed at the rear end of the fixing frame 3142 through a bearing, a screw rod is connected to the middle part of the gear rack 3144 through a thread fit, the end part of the screw rod is connected with the feeding plate 3143 through a bearing, and the gear rack 3144 is meshed with the feeding mechanism 315.
In order to prevent the frozen pathological tissues from falling off from the inside of the fixing frame 3142, the front end of the feeding plate 3143 is provided with inserting needles, the end parts of the inserting needles are of a convex structure, when the pathological tissues are not frozen, the pathological tissues can be accurately filled in gaps among the inserting needles, when the pathological tissues are frozen, the convex structure can effectively prevent the tissues from falling off, and after the frozen tissues are completely thawed, the pathological tissues can be effectively removed.
In order to ensure the uniform cutting of pathological tissues, a feeding mechanism 315 is provided in this embodiment, the feeding mechanism 315 includes a rotating shaft 3151, a connecting gear 3152, a rotating gear 3153 and a latch 3154, the rotating shaft 3151 is installed inside the moving frame 312 through a bearing, the connecting gear 3152 is uniformly provided on the rotating shaft 3151, the connecting gear 3152 is meshed with the gear frame 3144, the rotating gear 3153 is provided between adjacent connecting gears 3152, the rotating gear 3153 is meshed with the latch 3154, the latch 3154 is installed in a rectangular groove a1 on the inner side surface of the moving plate 3132, the number of the latches 3154 is multiple, the latch 3154 is connected with the rectangular groove through a pin shaft, and a torsion spring is provided on the pin shaft.
In the actual use process, the slicing mechanism 313 and the feeding mechanism 315 are matched with each other to realize the uniform feeding of the feeding plate 3143, when the slicing mechanism 313 moves upwards, the slicing mechanism 313 drives the rotating gear 3153 to rotate through the latch 3154, the rotating gear 3153 further drives the pathological tissue to feed in a gear matching mode, and when the slicing mechanism 313 moves downwards, the latch 3154 is not meshed with the rotating gear 3153.
Referring to fig. 9, it should be noted that the latch 3154 is rotatably disposed, the latch 3154 is located at the upper end inside the rectangular groove a1, when the slicing mechanism 313 moves downward, the upper end of the latch 3154 is stressed, the latch 3154 is stressed and contracts into the rectangular groove a1, when the slicing mechanism 313 moves upward, the lower end of the latch 3154 is stressed, the rectangular groove a1 can limit the latch 3154, and the latch 3154 can be engaged with the rotating gear 3153, so that the function of uniform feeding of pathological tissues can be realized.
In order to slice pathological tissues with different thicknesses, the number of the clamping teeth 3154 is increased or decreased to increase or decrease the feeding amount of the pathological tissues driven by the feeding mechanism 315, so that the function of slicing pathological tissues with different thicknesses can be realized.
Example three:
on the basis of the second embodiment, in order to accurately produce the pathological tissue under the closed-loop condition and prevent the pathological tissue from contacting with the external environment, the third embodiment is provided with a corresponding transfer unit 32.
Referring to fig. 2, the transfer unit 32 includes a vertical moving module 321, a horizontal moving module 322, a transfer assembly 323 and a guide frame 324, the vertical moving module 321 is symmetrically disposed on the right side of the middle of the housing frame 1, an electromagnet matched with the slicing unit 31 is disposed on the vertical moving module 321, the horizontal moving module 322 is symmetrically disposed in the middle of the housing frame 1, the transfer assembly 323 is mounted at the upper end of the horizontal moving module 322, the lower end of the transfer assembly 323 is closely attached to the guide frame 324, the guide frame 324 is mounted on the inner wall of the housing frame 1, and an upward inclined smooth surface is disposed on the left side of the guide frame 324.
In the actual use process, when the electromagnet is powered on, the vertical moving module 321 can drive the moving frame 312 to move downwards, so that the supporting bracket 317 moves downwards to the upper end of the transferring assembly 323, the slide glass on the supporting bracket 317 is in contact with the transferring assembly 323, then the horizontal moving module 322 drives the transferring assembly 323 to move leftwards, and the guide frame 324 can drive the transferring assembly 323 to move upwards, so that the slide glass on the transferring assembly 323 is attached to the cover glass under the closed-loop condition, and the pathological tissue slice between the slide glass and the cover glass is prevented from contacting the air environment.
It should be noted that the vertical moving module 321 and the horizontal moving module 322 are both electric sliding rails, and in the working process, the vertical moving module 321 and the horizontal moving module 322 have good moving effect and move stably.
In the actual use process, the guide frame 324 drives the transfer plate 3234 to move upwards through the guide rod 3232, so that the slide glass on the transfer plate 3234 can be accurately matched with the cover glass, and the extension spring 3233 plays a role in resetting, and is favorable for resetting the transfer plate 3234.
The suction holes uniformly formed in the upper surface of the transfer plate 3234 can accurately suck the glass slide tightly, and the glass slide is prevented from falling down when moving.
Referring to fig. 10, the support bracket 317 is uniformly provided with a placement groove corresponding to the transfer plate 3234, and the width of the transfer plate 3234 is smaller than that of the placement groove, so that the slide glass can be accurately dropped on the transfer plate 3234 when the support bracket 317 containing the slide glass moves downward.
It should be noted that, the standing groove upper end still is provided with the inductor, and the inductor is used for responding to whether there is the slide glass standing groove department to prevent that the slide glass from forgetting to place, avoid the pathological tissue after the section to drop inside freezer 2.
The implementation principle of the embodiment is as follows:
1: pathological tissue is cut into pieces, and pathological tissues to be detected are taken and accurately cut into rectangular structures, so that the pathological tissue cut pieces can accurately adapt to the middle part of the fixing frame 3142.
2: freezing is cut in advance, freezes pathological tissue through refrigeration module, carries out reciprocal section through slice mechanism to pathological tissue surface to reach the purpose of flattening pathological tissue surface, guarantee that the film after the cutting is in pathological tissue's middle part, the pathological tissue after cutting in advance drops inside collecting frame 316.
3: when the cutting blade 3134 is moved to the lowermost end, the negative pressure fan stops working, and the inclined thin slice accurately drops on the glass slide.
4: the slide is transferred, the vertical moving module 321 can drive the moving frame 312 to move downwards to the upper end of the transfer assembly 323, the slide on the support bracket 317 is in contact with the transfer assembly 323, then the horizontal moving module 322 drives the transfer assembly 323 to move leftwards, and the guide frame 324 can drive the transfer assembly 323 to move upwards, so that the slide on the transfer assembly 323 is attached to the cover glass under the closed-loop condition, and the pathological tissue slice between the slide and the cover glass is prevented from contacting the air environment.
The embodiments of the present invention are preferred embodiments of the present invention, and the scope of the present invention is not limited by these embodiments, so: equivalent changes made according to the structure, shape and principle of the invention shall be covered by the protection scope of the invention.
Claims (10)
1. The utility model provides a pathological section gene identification closed loop protection observation platform, includes casing frame (1) and sets up in freezer (2) of casing frame (1) upper end, and casing frame (1) right side is provided with the observation area, installs control panel on the inclined plane on casing frame (1) right side, and its characterized in that still includes: closed loop section subassembly (3), closed loop section subassembly (3) run through the setting in the inside cavity of casing frame (1) and freezer (2), closed loop section subassembly (3) and casing frame (1) and freezer (2) sealing connection, closed loop section subassembly (3) are connected with control panel electricity, closed loop section subassembly (3) are used for closing the frozen section of the pathological tissue under the ring attitude, the pathological tissue thin slice after the section is transferred to observation area below and is used for the optical microscopic observation.
2. The closed-loop protection observation platform for pathological section gene identification according to claim 1, wherein the closed-loop protection observation platform comprises: the observation area is provided with a placing plate (11) in a detachable mode, the placing plate (11) is of a rectangular structure, negative pressure grooves are evenly formed in the placing plate (11), the negative pressure grooves are matched with the cover glass, and negative pressure holes are evenly formed in the negative pressure grooves.
3. The closed-loop protection observation platform for pathological section gene identification according to claim 1, wherein the closed-loop protection observation platform comprises: the closed-loop slicing assembly (3) comprises a slicing unit (31) and a transferring unit (32), the slicing unit (31) is located in a cavity in the middle of the freezing box (2), the transferring unit (32) is located in a cavity in the middle of the shell frame (1), the slicing unit (31) is detachably inserted into the freezing box (2), and the upper end of the transferring unit (32) is matched with the slicing unit (31).
4. The closed-loop protection observation platform for pathological section gene identification according to claim 3, wherein: the slicing unit (31) comprises an inserting frame (311), a moving frame (312), a slicing mechanism (313), a supporting plate (314), a feeding mechanism (315), a collecting frame (316) and a supporting bracket (317), wherein the inserting frame (311) is of a U-shaped structure, the moving frame (312) is arranged on the inserting frame (311), the moving frame (312) is in clamping fit with the inserting frame (311), the slicing mechanism (313) is arranged at the upper end of the front end face of the moving frame (312), the uniform supporting plate (314) is arranged in the middle of the moving frame (312), the rear end of the supporting plate (314) is connected with the slicing mechanism (313) through the feeding mechanism (315), the collecting frame (316) is arranged at the lower end of the front end face of the moving frame (312), and the supporting bracket (317) used for placing glass slides is arranged at the lower end of the moving frame (312).
5. The closed-loop protection observation platform for pathological section gene identification according to claim 4, wherein: slicer mechanism (313) are including electronic slider (3131), movable plate (3132), link (3133) and cutting blade (3134), removal frame (312) preceding terminal surface on the symmetry be provided with the spout, be connected with electronic slider (3131) through sliding fit's mode in the spout, be provided with movable plate (3132) on electronic slider (3131), movable plate (3132) are the I shape structure, install link (3133) between movable plate (3132) front end, evenly be provided with cutting blade (3134) on link (3133).
6. The pathological section gene identification closed-loop protection observation platform of claim 5, which is characterized in that: the cutting blade (3134) is obliquely arranged, and orifices are uniformly arranged on the cutting blade (3134) and connected with the negative pressure fan.
7. The pathological section gene identification closed-loop protection observation platform of claim 5, which is characterized in that: the supporting plate (314) comprises a fan grating (3141), a fixed frame (3142), a feeding plate (3143) and a gear carrier (3144), the fan grating (3141) is uniformly installed in a groove at the front end of the moving frame (312), the fan grating (3141) is made of heat-conducting materials, the fixed frame (3142) is installed in the middle of the fan grating (3141), the feeding plate (3143) is arranged in the middle of the fixed frame (3142) in a sliding mode, inserting pins are uniformly arranged on the front end face of the feeding plate (3143), the gear carrier (3144) is installed at the rear end of the fixed frame (3142) through a bearing, a screw rod is connected to the middle of the gear carrier (3144) in a threaded fit mode, the end of the screw rod is connected with the feeding plate (3143) through the bearing, and the gear carrier (3144) is meshed with the feeding mechanism (315).
8. The closed-loop protection observation platform for pathological section gene identification according to claim 7, wherein: the feeding mechanism (315) comprises a rotating shaft (3151), a connecting gear (3152), a rotating gear (3153) and a latch (3154), the rotating shaft (3151) is installed inside the moving frame (312) through a bearing, the connecting gear (3152) is uniformly arranged on the rotating shaft (3151), the connecting gear (3152) is meshed with a gear carrier (3144), the rotating gear (3153) is arranged between adjacent connecting gears (3152), the rotating gear (3153) is meshed with the latch (3154), the latch (3154) is installed in a rectangular groove a1 on the inner side surface of the moving plate (3132), the number of the latches (3154) is multiple, the latch (3154) is connected with the rectangular groove through a pin shaft, and a torsion spring is arranged on the pin shaft.
9. The closed-loop protection observation platform for pathological section gene identification according to claim 3, wherein: transfer unit (32) including vertical migration module (321), lateral shifting module (322), transfer subassembly (323) and leading truck (324), casing frame (1) middle part right side symmetry is provided with vertical migration module (321), be provided with on vertical migration module (321) with section unit (31) complex electro-magnet, casing frame (1) middle part symmetry is provided with lateral shifting module (322), lateral shifting module (322) upper end is installed and is transferred subassembly (323), transfer subassembly (323) lower extreme and leading truck (324) are hugged closely, leading truck (324) are installed on casing frame (1) inner wall, leading truck (324) left side is provided with the smooth surface of tilt up.
10. The closed-loop protection observation platform for pathological section gene identification according to claim 9, wherein: transfer subassembly (323) including crossbeam board (3231), guide arm (3232), expanding spring (3233) and transfer board (3234), install crossbeam board (3231) between lateral shifting module (322), evenly be provided with the through-hole on crossbeam board (3231), it is provided with guide arm (3232) to slide in the through-hole, guide arm (3232) lower extreme is the smooth surface, is provided with expanding spring (3233) on guide arm (3232), installs the diaphragm between guide arm (3232) upper end, and transfer board (3234) are evenly installed to the diaphragm upper end, and transfer board (3234) is tilt up structure, and transfer board (3234) surface evenly is provided with inhales the hole.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN206348196U (en) * | 2016-12-27 | 2017-07-21 | 黄河科技学院 | A kind of histopathologic slide makes platform |
| CN214055388U (en) * | 2020-11-30 | 2021-08-27 | 新疆医科大学第三附属医院 | Pathology department observes pathological section's auxiliary device |
| CN113670695A (en) * | 2021-08-26 | 2021-11-19 | 广东医科大学 | Pathological microtome capable of slicing in two sides |
| CN215050237U (en) * | 2021-04-23 | 2021-12-07 | 黄河科技学院 | Tissue slice culture apparatus |
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2022
- 2022-05-17 CN CN202210534423.6A patent/CN114636700B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN206348196U (en) * | 2016-12-27 | 2017-07-21 | 黄河科技学院 | A kind of histopathologic slide makes platform |
| CN214055388U (en) * | 2020-11-30 | 2021-08-27 | 新疆医科大学第三附属医院 | Pathology department observes pathological section's auxiliary device |
| CN215050237U (en) * | 2021-04-23 | 2021-12-07 | 黄河科技学院 | Tissue slice culture apparatus |
| CN113670695A (en) * | 2021-08-26 | 2021-11-19 | 广东医科大学 | Pathological microtome capable of slicing in two sides |
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Address after: Floor 8, Building 4, No. 128 Shangnan East Road, Huangpu Community, Xinqiao Street, Bao'an District, Shenzhen City, Guangdong Province, 518000 Patentee after: HUACHENYANG (SHENZHEN) TECHNOLOGY Co.,Ltd. Address before: 518000 8th and 11th floors of No.4 factory building, 128 Shangnan East Road, Huangpu Community, Xinqiao street, Bao'an District, Shenzhen City, Guangdong Province Patentee before: HUACHENYANG (SHENZHEN) TECHNOLOGY Co.,Ltd. |