CN114634871A

CN114634871A - Culture device and culture method of vaccine strain

Info

Publication number: CN114634871A
Application number: CN202011483895.0A
Authority: CN
Inventors: 李浩然; 孙婷; 孙笑非
Original assignee: Nanjing Institute Of Life And Health Sciences
Current assignee: Nanjing Institute Of Life And Health Sciences
Priority date: 2020-12-15
Filing date: 2020-12-15
Publication date: 2022-06-17

Abstract

The invention belongs to the field of vaccine research, and particularly discloses a culture device for vaccine strains, which comprises an incubator, wherein the incubator is internally divided into a plurality of culture chambers, and the bottom of each culture chamber is provided with a taking and placing plate with a drawing structure; a temperature control chamber is formed at one side of the incubator, a refrigerating system and an air purifying system are installed in the temperature control chamber, and an electric heating pipe is installed at the top of each incubator chamber; the culture tray is placed on the taking and placing plate, and a plurality of stacked and assembled culture boxes are arranged on the culture tray at equal intervals. The invention forms a plurality of independent culture chambers in the same incubator, can provide independent isolation spaces for different microbial strains, and effectively avoids cross contamination; the culture boxes can be taken and placed independently and can be taken and transferred in batches; the temperature environment control system is arranged in the incubator, so that the temperature environment can be set independently, temperature balance adjustment can be performed uniformly, and synchronous culture of various microbial strains can be met.

Description

Culture device and culture method of vaccine strain

Technical Field

The invention relates to the field of vaccine research, in particular to a culture device and a culture method of a vaccine strain.

Background

The vaccine is an automatic immune preparation for preventing infectious diseases, which is prepared by artificially attenuating, inactivating or utilizing transgenosis methods for pathogenic microorganisms (such as bacteria, rickettsia, viruses and the like) and metabolites thereof. The vaccine retains the property of pathogenic bacteria to stimulate the immune system of an animal. When an animal body contacts the pathogen without harm, the immune system can generate certain protective substances, such as immune hormone, active physiological substances, special antibodies and the like; when the animal is exposed to the pathogenic bacteria again, the immune system of the animal will follow its original memory and produce more protective substances to prevent the pathogenic bacteria from harming.

The vaccine strain is cultured in a relatively isolated environment because it is necessary to prevent the microbial strain from being contaminated by the outside environment and also to prevent the strain from being contaminated by the outside environment. However, the existing isolation boxes for culturing microbial strains only aim at isolating the culture boxes from the outside, but neglect that cross contamination can occur among different microorganisms in the same culture box, and further the purity of strain culture is influenced.

Disclosure of Invention

The present invention aims to provide a culture apparatus and a culture method for vaccine strains, which solve the problems of the background art.

In order to achieve the purpose, the invention provides the following technical scheme: a culture device for vaccine strains comprises an incubator, wherein the incubator is internally divided into a plurality of culture chambers by a plurality of layers of partition plates which are distributed at equal intervals from top to bottom, and the bottom of each culture chamber is respectively provided with a taking and placing plate with a drawing structure; one side of the incubator is outwards expanded and is independently separated to form a temperature control chamber, a refrigerating system and an air purification system are installed in the temperature control chamber, the output end of the refrigerating system is connected with a cold air conveying pipe, a plurality of air supply pipes which are respectively introduced into the respective incubator are connected to the branch of the cold air conveying pipe, a wind equalizing plate communicated with the air supply pipes is respectively installed in the respective incubator, the input end of the refrigerating system is connected with the air purification system, and an electric heating pipe is respectively installed at the top part in the respective incubator; the culture tray is placed on the taking and placing plate, and a plurality of stacked and assembled culture boxes are arranged on the culture tray at equal intervals.

Preferably, the taking and placing plates are connected with the corresponding partition plates in a sliding mode, the outer ends of the taking and placing plates are vertically turned upwards to form a door plate for sealing the opening of the culture chamber, a handle is mounted on the door plate, and a layer of sealing gasket is coated on the periphery of the door plate and on the two sides of the taking and placing plates.

Preferably, the air-equalizing plate is of a hollow structure, a plurality of air outlets are distributed on one side surface of the air-equalizing plate facing the interior of the culture chamber, and each air supply pipe is provided with an electric control valve.

Preferably, an air inlet is formed in one side of the temperature control chamber, the input end of the air purification system is communicated with the air inlet, a pressure balance pipe communicated with the outside is respectively installed in each culture chamber, and a pressure release valve is installed on each pressure balance pipe and is a single check valve.

Preferably, a temperature sensor and a pressure sensor are attached to each of the culture chambers.

Preferably, the center of the taking and placing plate is provided with at least one positioning hole, the bottom surface of the culture tray is correspondingly provided with a positioning lug embedded into the positioning hole, and two sides of each culture tray are respectively provided with an ear plate convenient to take.

Preferably, the culture box includes the culture dish and the culture lid of configuration setting on the culture dish, and the culture lid top surface is provided with the groove of assembling that supplies the invagination structure of culture dish embedding, cultivates the equidistant a plurality of constant head tanks that are the same with assembling the groove structure that are provided with on the dish.

Preferably, the control panel is installed outside the temperature control chamber and is connected with the control ends of the refrigeration system, the air purification system, the electric control valve, the pressure release valve, the temperature sensor, the pressure sensor and the electric heating pipe respectively.

Preferably, the whole incubator and the partition plate are made of heat insulation materials.

The invention also provides a culture method of the vaccine strain, which comprises the following specific steps:

s1: the culture boxes inoculated with the vaccine strains are placed on different culture plates in a classified manner, the culture boxes of the same batch can be stacked and embedded in positioning grooves of the culture plates, then the culture plates are placed on a taking and placing plate of a culture chamber in a classified manner, positioning lugs at the bottom of the culture plates are embedded into positioning holes of the taking and placing plate, and the taking and placing plate is pushed into the culture chamber through a handle to form a sealed state;

s2: temperature values in different cultivation rooms are set through the control panel, the refrigeration system, the air purification system and the electric heating pipe regulate and control the temperature according to instructions, a temperature sensor in the cultivation room acquires temperature data in the cultivation room in real time and sends the data to the control panel, and the control panel judges temperature rise or temperature fall according to the actual temperature data, so that the refrigeration system, the air purification system or the electric heating pipe is triggered to start;

s3: the pressure sensors acquire pressure data in each culture chamber in real time and send the data to the control panel, when the pressure is higher than a set threshold range, the pressure release valves are triggered to open, the pressure in the culture chambers is balanced, and the pressure release valves are closed until the pressure reaches the set threshold;

s4: when daily record or transfer the culture box, can select the culture box of corresponding batch to carry out independent taking as required, also can take the culture box after the pile in batches, also can carry out the transfer of whole layer through the culture dish.

Compared with the prior art, the invention has the beneficial effects that: .

The invention forms a plurality of independent culture chambers in the same incubator, can provide independent isolation spaces for different microbial strains, and effectively avoids cross contamination; the culture tray is arranged on the partition plate, so that the culture boxes can be integrally taken and placed in batches; the culture box with the splicing and combining structure can be taken and placed independently, can be taken and transferred in batches, and is convenient to use; a temperature environment control system is arranged in the incubator, so that the temperature environment can be set independently, temperature balance adjustment can be performed uniformly, and synchronous culture of various microbial strains can be met; air purification system is used for strengthening and purifying the air entering the incubator, and the sterile environment in the incubator is further guaranteed.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic view showing the inside structure of the incubator of the present invention;

FIG. 3 is a schematic view showing a specific stack of the culture cassette of the present invention;

FIG. 4 is a schematic view showing a specific structure of the culture dish of the present invention.

In the figure: 1. a base plate; 2. a partition plate; 3. a culture chamber; 4. taking and placing the plate; 5. a door panel; 6. a second diversion slope; 7. a gasket; 8. a temperature control chamber; 9. a refrigeration system; 10. a cold air delivery pipe; 11. an air supply pipe; 12. a wind equalizing plate; 13. an air outlet; 14. an electrically controlled valve; 15. an air purification system; 16. an air inlet; 17. a pressure balance tube; 18. a pressure relief valve; 19. a temperature sensor; 20. a pressure sensor; 21. an electric heating tube; 22. a culture tray; 23. positioning holes; 24. positioning the bump; 25. a culture dish; 26. a culture cover; 27. assembling the grooves; 28. positioning a groove; 29. an ear plate; 30. and a control panel.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "vertical", "upper", "lower", "horizontal", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

Example 1: referring to fig. 1-4, the present invention provides a technical solution: a vaccine strain culture device comprises an incubator 1, wherein the incubator 1 is internally divided into a plurality of culture chambers 3 by a plurality of layers of partition plates 2 which are distributed at equal intervals from top to bottom, and the bottoms of the culture chambers 3 are respectively provided with a taking and placing plate 4 with a drawing structure; one side of the incubator 1 is expanded outwards and is independently separated to form a temperature control chamber 8, a refrigerating system 9 and an air purification system 15 are installed in the temperature control chamber 8, the output end of the refrigerating system 9 is connected with a cold air conveying pipe 10, the upper branch of the cold air conveying pipe 10 is connected with a plurality of air feeding pipes 11 which are respectively led into the incubation chambers 3, an air equalizing plate 12 communicated with the air feeding pipes 11 is respectively installed in each incubation chamber 3, the input end of the refrigerating system 9 is connected with the air purification system 15, and an electric heating pipe 21 is respectively installed at the top in each incubation chamber 3; the culture tray 22 is placed on the taking and placing plate 4, and a plurality of stacked and assembled culture boxes are arranged on the culture tray 22 at equal intervals.

In this embodiment, get and put

board

4 and 2 sliding connection of corresponding baffle, each get and put 4 outer ends of board and upwards turn over perpendicularly and form 3 opening part confined door plant 5 with the culture chamber, install handle 6 on the door plant 5, door plant 5 periphery and get and put 4 both sides of board cladding of one deck sealed pad 7.

In this embodiment, the air-equalizing plate 12 is hollow, and has a plurality of air outlets 13 distributed on a side surface facing the inside of the culture chamber 3, and each air supply pipe 11 is provided with an electrically controlled valve 14.

In this embodiment, an air inlet 16 is provided at one side of the temperature control chamber 8, an input end of the air purification system 15 is communicated with the air inlet 16, a pressure balance pipe 17 communicated with the outside is respectively installed in each culture chamber 3, and a pressure release valve 18 is installed on the pressure balance pipe 17, wherein the pressure release valve 18 is a single check valve.

In the present embodiment, a temperature sensor 19 and a pressure sensor 20 are mounted in each of the culture chambers 3.

In this embodiment, the center of the access plate 4 is provided with at least one positioning hole 23, the bottom surface of the cultivation dish 22 is correspondingly provided with a positioning protrusion 24 embedded in the positioning hole 23, and two sides of each cultivation dish 22 are respectively provided with an ear plate 29 convenient for taking.

In this embodiment, the culture box includes a culture dish 25 and a culture cover 26 disposed on the culture dish 25, wherein the top surface of the culture cover 26 is provided with a splicing groove 27 having an inward-recessed structure for the culture dish 25 to be inserted, and the culture tray 22 is provided with a plurality of positioning grooves 28 having the same structure as the splicing groove 27 at equal intervals.

In this embodiment, a control panel 30 is installed outside the temperature controlled chamber 8, and the control panel 30 is connected to the refrigeration system 9, the air purification system 15, the electronic control valve 14, the pressure relief valve 18, the temperature sensor 19, the pressure sensor 20, and the control end of the electric heating pipe 21, respectively.

In this embodiment, the whole incubator 1 and the partition 2 are made of heat insulating material.

Example 2: the invention also provides a culture method of the vaccine strain, which comprises the following specific steps:

s1: the culture boxes inoculated with the vaccine strains are placed on different culture plates 22 in a classified manner, the culture boxes in the same batch can be stacked and embedded in positioning grooves 28 of the culture plates 22, then the culture plates 22 are placed on the taking and placing plate 4 of the culture chamber 3 in a classified manner, positioning bumps 24 at the bottoms of the culture plates are embedded into positioning holes 23 of the taking and placing plate 4, and the taking and placing plate 4 is pushed into the culture chamber 3 through a handle 6 to form a sealed state.

S2: temperature values in different culture chambers 3 are set through the control panel 30, the refrigeration system 9, the air purification system 15 and the electric heating pipe 21 perform temperature control according to instructions, the temperature sensor 19 in the culture chamber 3 acquires temperature data in the culture chamber 3 in real time and sends the data to the control panel 30, and the control panel 30 judges temperature rise or temperature fall according to actual temperature data, so that the refrigeration system 9, the air purification system 15 or the electric heating pipe 21 is triggered to start;

when refrigerating system 9 starts, its input gets into and puts into the air and purify the filtration through air purification system 15 earlier, because incubator 1 itself just places in sterile environment, strengthens purifying once more to the air current that gets into in culture room 3, effectively guarantees the sterile condition in the incubator 1.

S3: the pressure sensor 20 acquires pressure data in each culture chamber 3 in real time, sends the data to the control panel 30, and when the pressure is higher than a set threshold range, the pressure release valve 18 is triggered to open, the pressure in the culture chambers 3 is balanced, and the pressure release valve 18 is closed until the pressure reaches the set threshold;

s4: when daily records or culture boxes are transferred, culture boxes of corresponding batches can be selected according to requirements to be independently taken, stacked culture boxes can be taken in batches, and the whole layer can be transferred through the culture tray 22

It is worth noting that: the whole device realizes control to the device through the control panel 30, the control panel 30 is a touch screen structure, and since the control matched device is a common device, the device belongs to the existing mature technology, and the electrical connection relation and the specific circuit structure are not repeated herein.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A culture apparatus for vaccine strains comprises an incubator (1), and is characterized in that the incubator (1) is divided into a plurality of culture chambers (3) by a plurality of layers of partition plates (2) which are distributed at equal intervals from top to bottom, and the bottoms of the culture chambers (3) are respectively provided with a taking and placing plate (4) with a drawing structure; one side of the incubator (1) is expanded outwards and is independently separated to form a temperature control chamber (8), a refrigerating system (9) and an air purification system (15) are installed in the temperature control chamber (8), the output end of the refrigerating system (9) is connected with a cold air conveying pipe (10), the cold air conveying pipe (10) is connected with a plurality of air supply pipes (11) which are respectively introduced into the cultivation chambers (3) in a branching mode, an air equalizing plate (12) communicated with the air supply pipes (11) is respectively installed in each cultivation chamber (3), the input end of the refrigerating system (9) is connected with the air purification system (15), and an electric heating pipe (21) is respectively installed at the top in each cultivation chamber (3); the culture tray (22) is placed on the taking and placing plate (4), and a plurality of stacked and assembled culture boxes are arranged on the culture tray (22) at equal intervals.

2. The apparatus for culturing a vaccine strain according to claim 1, wherein: the taking and placing plates (4) are connected with the corresponding partition plates (2) in a sliding mode, the outer ends of the taking and placing plates (4) are upwards and vertically turned over to form door plates (5) for sealing openings of the culture chambers (3), handles (6) are installed on the door plates (5), and the peripheries of the door plates (5) and the two sides of the taking and placing plates (4) are coated with a layer of sealing gaskets (7).

3. The apparatus for culturing a vaccine strain according to claim 1, wherein: the air-equalizing plate (12) is of a hollow structure, a plurality of air outlets (13) are distributed on one side surface of the air-equalizing plate facing the interior of the culture chamber (3), and each air supply pipe (11) is provided with an electric control valve (14).

4. The apparatus for culturing a vaccine strain according to claim 1, wherein: an air inlet (16) is formed in one side of the temperature control chamber (8), the input end of the air purification system (15) is communicated with the air inlet (16), a pressure balance pipe (17) communicated with the outside is installed in each culture chamber (3), a pressure release valve (18) is installed on each pressure balance pipe (17), and each pressure release valve (18) is a single-phase check valve.

5. The apparatus for culturing a vaccine strain according to claim 1, wherein: a temperature sensor (19) and a pressure sensor (20) are respectively installed in each culture chamber (3).

6. The apparatus for culturing a vaccine strain according to claim 1, wherein: the center of the taking and placing plate (4) is provided with at least one positioning hole (23), the bottom surface of the culture disc (22) is correspondingly provided with a positioning lug (24) embedded in the positioning hole (23), and two sides of each culture disc (22) are respectively provided with an ear plate (29) convenient to take.

7. The apparatus for culturing a vaccine strain according to claim 1, wherein: the culture box comprises a culture dish (25) and a culture cover (26) which is arranged on the culture dish (25) in a matching mode, wherein the top surface of the culture cover (26) is provided with an assembling groove (27) of an invagination structure for embedding the culture dish (25), and a plurality of positioning grooves (28) which are identical to the assembling groove (27) in structure are arranged on the culture dish (22) at equal intervals.

8. The apparatus for culturing a vaccine strain according to claim 1, wherein: the control device is characterized in that a control panel (30) is installed outside the temperature control chamber (8), and the control panel (30) is respectively connected with the control ends of the refrigerating system (9), the air purification system (15), the electric control valve (14), the pressure release valve (18), the temperature sensor (19), the pressure sensor (20) and the electric heating pipe (21).

9. The apparatus for culturing a vaccine strain according to claim 1, wherein: the whole incubator (1) and the partition plate (2) are made of heat insulation materials.

10. The culture method of the vaccine strain is characterized by comprising the following specific steps:

s1: the culture boxes inoculated with the vaccine strains are placed on different culture trays (22) in a classified manner, the culture boxes of the same batch can be stacked and embedded in positioning grooves (28) of the culture trays (22), then the culture trays (22) are placed on a taking and placing plate (4) of a culture chamber (3) in a classified manner, positioning bumps (24) at the bottoms of the culture trays are embedded into positioning holes (23) of the taking and placing plate (4), and the taking and placing plate (4) is pushed into the culture chamber (3) through a handle (6) to form a sealed state;

s2: temperature values in different culture rooms (3) are set through a control panel (30), a refrigeration system (9), an air purification system (15) and an electric heating pipe (21) regulate and control the temperature according to instructions, a temperature sensor (19) in the culture room (3) acquires temperature data in the culture room (3) in real time and sends the data to the control panel (30), and the control panel (30) judges temperature rise or temperature fall according to actual temperature data, so that the refrigeration system (9), the air purification system (15) or the electric heating pipe (21) is triggered to start;

s3: the pressure sensors (20) acquire pressure data in each culture chamber (3) in real time and send the data to the control panel (30), when the pressure is higher than a set threshold range, the pressure release valves (18) are triggered to be opened, the pressure in the culture chambers (3) is balanced, and the pressure release valves (18) are closed until the pressure reaches the set threshold;

s4: when daily records or culture boxes are transferred, culture boxes of corresponding batches can be selected according to needs to be independently taken, stacked culture boxes can be taken in batches, and the whole layer can be transferred through the culture tray (22).