CN114606154A - Tail vegetable wastewater recycling microbial inoculum and application thereof in preparation of plant nutrient solution - Google Patents
Tail vegetable wastewater recycling microbial inoculum and application thereof in preparation of plant nutrient solution Download PDFInfo
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
- C05F9/04—Biological compost
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
The invention discloses a waste water recycling microbial inoculum HQY-of-002 prepared from Bacillus licheniformis (Bacillus licheniformis)Bacillus licheniformis) ACCC 01050, Bacillus amyloliquefaciens (A)Bacillus amyloliquefaciens) CCTCC NO: m2017040, vegetable bacillus (B.plantarum) (B.plantarum)Bacillus oleronius) CCTCC NO: m2017039 and Pichia pastoris (Pichia farinose) CCTCC NO: m2017044. The strain in the microbial agent disclosed by the invention is selected from strains which are not used for toxicological tests in general technical guidelines for biological safety of microbial fertilizers (NY 1109-2006) and strains which are issued by Ministry of health and informed by List of strains which can be used for food, does not contain heavy metals and toxic and harmful chemical substances, and solid products are non-toxic and non-irritant. The waste water recycling microbial inoculum of the invention can decompose polysaccharide, protein, fiber and other substances in the waste water of the kale, can ferment the waste water of the kale into plant nutrient solution, and the fermented plant nutrient solution has no external chemical medicine, is rich in a large amount of trace elements required by plant growth, and has a promoting effect on the plant growth.
Description
Technical Field
The invention relates to a waste water recycling microbial inoculum of waste water of Chinese cabbage and application thereof in preparing plant nutrient solution by treating plant waste water with microorganisms.
Background
China is a big country where waste of the tailstocks is generated, the water content of the waste of the tailstocks is as high as more than 90%, and the waste of the tailstocks inevitably generates a large amount of high-concentration wastewater, namely the waste of the tailstocks in the process of realizing harmlessness, reduction and recycling. COD in the waste water of the tailgating vegetable production is up to 30000mg/L, ammonia nitrogen is up to 500mg/L, and total phosphorus is up to 120mg/L, if the waste water is used for sewage treatment, the waste water faces huge treatment pressure no matter the waste water reaches the surface water discharge standard or the nano-tube standard.
The microorganisms which are used as natural decomposers can decompose the waste of the tailed vegetable into components which can be absorbed by the plants and return the components to the soil, so if the strains which can decompose the substances such as polysaccharide, protein, fiber and the like in the tailed vegetable are adopted to convert the components into the substances which can be absorbed by the plants, the environmental pressure is greatly reduced, the waste can be changed into the valuable, and the method has important significance for the vegetable industry.
Disclosure of Invention
The invention aims to provide a waste water recycling microbial inoculum of waste water of Chinese cabbage and application thereof in preparing plant nutrient solution by treating plant waste water with recycling microorganisms.
The technical scheme adopted by the invention is as follows:
a bacteriacide HQY-of-002 for reclaiming the waste water of cabbage is prepared from Bacillus licheniformis (Bacillus licheniformis)Bacillus licheniformis ) ACCC 01050, Bacillus amyloliquefaciens (A), (B) and (C)Bacillus amyloliquefaciens) CCTCC NO: m2017040, vegetable bacillus (B.plantarum) (B.plantarum)Bacillus oleronius) CCTCC NO: m2017039 and Pichia pastoris (Pichia farinose) CCTCC NO: m2017044.
The strain in the microbial agent disclosed by the invention is selected from strains which are not used for toxicological tests in general technical guidelines for biological safety of microbial fertilizers (NY 1109-2006) and strains which are issued by Ministry of health and informed by List of strains which can be used for food, does not contain heavy metals and toxic and harmful chemical substances, and solid products are non-toxic and non-irritant. The microbial inoculum has the advantages of no secondary pollution to the environment and no additional burden in practical application, belongs to an environment-friendly compatible microbial inoculum product, and is simple, convenient and efficient to store, transport and put in. The process is simple to operate, no external chemical is added, and the fermented plant nutrient solution is free of external chemical, rich in a large amount of trace elements required by plant growth and capable of promoting plant growth.
Preferably, the composition is as follows according to the ratio of the number of viable bacteria cultured to the middle and later logarithmic stages: bacillus licheniformis (Bacillus licheniformis ) ACCC 0105025-35%, Bacillus amyloliquefaciens (Bacillus amyloliquefaciens)Bacillus amyloliquefaciens) CCTCC NO: m201704025-35%, and Bacillus cereus (Bacillus cereus)Bacillus oleronius) CCTCC NO: m201703920-30%, Pichia pastoris (A)Pichia farinose）CCTCC NO：M2017044 15%~25%。
More preferably, the waste water recycling microbial inoculum of the waste water of the tailed vegetables comprises the following components: bacillus licheniformis (Bacillus licheniformis ) ACCC 0105030%, Bacillus amyloliquefaciens (B.amyloliquefaciens) (B.amyloliquefaciens)Bacillus amyloliquefaciens) CCTCC NO: m201704025%, Bacillus cereus (B.plantarum) (B.cereus)Bacillus oleronius) CCTCC NO: m201703925%, Pichia pastoris (Pichia farinose）CCTCC NO：M2017044 20%。
The preparation method of the waste water recycling microbial inoculum HQY-of-002 of the invention comprises the following steps: inoculating each strain into a liquid strain activation culture medium, carrying out aerobic activation for 10-16 h at 30-36 ℃ to prepare a seed activation solution, inoculating the seed activation solution into the seed culture medium according to the inoculation amount of 1-3% by volume ratio, carrying out aerobic fermentation for 12-18 h at 30-36 ℃ to prepare a seed solution, mixing the seed solutions according to the ratio of each strain, inoculating the seed solution into a strain fermentation culture medium according to the inoculation amount of 3-5% by volume ratio, and carrying out aerobic fermentation for 18-24 h at 30-35 ℃ to prepare the tail vegetable wastewater recycling microbial inoculum HQY-of-002.
The strain activation culture medium of the waste water recycling microbial inoculum HQY-of-002 comprises the following components: 0.8% -1.2% (w/v, 1% means that 100mL of the culture medium contains 1g of the substance, the same below) peptone, 0.4% -0.6% yeast extract, 1.8% -2.2% glucose, 0.8% -1.2% sodium chloride, 1.8% -2.2% dipotassium hydrogen phosphate, 0.8% -1.2% ammonium chloride, 0.18% -0.22% magnesium sulfate heptahydrate, 0.048% -0.052% manganese sulfate, the pH value is adjusted to about 7.0, and the culture medium is sterilized for 30 minutes at 115 ℃. The seed culture medium comprises the following components: 1.8-2.2% of peptone, 0.8-1.2% of glucose, 0.8-1.2% of molasses, 0.8-1.2% of sodium chloride, 1.8-2.2% of dipotassium hydrogen phosphate, 1.8-2.2% of ammonium chloride, 0.18-0.22% of magnesium sulfate heptahydrate, 0.048-0.052% of manganese sulfate, adjusting the pH to about 7.0, and sterilizing at 115 ℃ for 30 minutes. The fermentation medium is characterized by comprising the following components: 60 to 70 percent of waste water of the vegetable tails, 0.8 to 1.2 percent of molasses, 0.4 to 0.6 percent of peptone, 0.5 to 0.8 percent of sodium chloride, 1.8 to 2.2 per mill of dipotassium hydrogen phosphate, 0.18 to 0.22 per mill of magnesium sulfate heptahydrate, adjusting the pH to about 7.0, and sterilizing for 30 minutes at the temperature of 115 ℃.
The invention also relates to application of the waste water recycling microbial inoculum of the waste water of the tailed vegetables in preparation of plant nutrient solution by treating plant waste water with recycling microorganisms.
Specifically, the plant wastewater is plant wastewater except for bactericidal plants.
Specifically, the application is as follows: adding the waste water recycle microbial inoculum HQY-of-002 into plant waste water, carrying out aerobic fermentation at 30-37 ℃, controlling dissolved oxygen to be 2-4 mg/L, fermenting for 3-6 days, and diluting to COD (chemical oxygen demand) to be 100-1000 mg/L after the fermentation is finished, thus obtaining the plant nutrient solution.
Preferably, the aerobic fermentation is carried out at 33-36 ℃.
The invention has the following beneficial effects: the waste water recycling microbial inoculum of the invention can decompose polysaccharide, protein, fiber and other substances in the waste water of the kale, can ferment the waste water of the kale into plant nutrient solution, and the fermented plant nutrient solution has no external chemical medicine, is rich in a large amount of trace elements required by plant growth, and has a promoting effect on the plant growth.
Drawings
FIG. 1 is a photograph of example 4 at day 4 of a seed germination experiment;
FIG. 2 is a comparison of germination indices of seeds at day 5 of example 4;
FIG. 3 is a photograph of example 4 on day 5 of a seed germination experiment.
(V) detailed description of the preferred embodiments
For the purpose of enhancing understanding of the present invention, the present invention will be described in further detail with reference to specific examples, which are provided for illustration only and are not intended to limit the scope of the present invention.
Example 1: preparation of waste water recycling microbial agent HQY-of-002 of waste water of Brassica oleracea
The microbial inoculum is prepared by the following steps:
(1) taking out each strain preserved in the glycerol freezing tube from a refrigerator at the temperature of minus 80 ℃, melting in an ultra-clean workbench sterilized by ultraviolet, and aseptically sucking 1 ml by a pipette and inoculating into a sterilized 10ml liquid strain activation culture medium for activation.
(2) The preparation method of the liquid strain activation culture medium used in the preparation step (1) of the microbial inoculum comprises the following steps: 1.0 percent of peptone, 0.5 percent of yeast extract, 2.0 percent of glucose, 1.0 percent of sodium chloride, 2.0 thousandth of dipotassium hydrogen phosphate, 1.0 thousandth of ammonium chloride, 0.2 thousandth of magnesium sulfate heptahydrate and 0.05 thousandth of manganese sulfate, and the pH is adjusted to be about 7.0. And the medium was sterilized at 115 ℃ for 30 minutes.
(3) After the strain in the step (1) of preparing the microbial inoculum is inoculated into an activation culture medium, aerobic activation is carried out for 10-16 h at 30-36 ℃, and then an activation solution can be prepared.
(4) And (3) absorbing the activated strain activating solution in the step (3) under an aseptic condition, inoculating the activated strain activating solution into a seed culture medium by 3 percent of inoculation amount, and carrying out aerobic fermentation at 30-36 ℃ for 12 hours to prepare a seed solution.
(5) The preparation method of the seed culture medium used in the step (4) comprises the following steps: 2.0 percent of peptone, 1.0 percent of glucose, 1.0 percent of molasses, 1.0 percent of sodium chloride, 2.0 thousandth of dipotassium hydrogen phosphate, 2.0 thousandth of ammonium chloride, 0.2 thousandth of magnesium sulfate heptahydrate and 0.05 thousandth of manganese sulfate, and the pH is adjusted to be about 7.0. And the medium was sterilized at 115 ℃ for 30 minutes.
(6) Sucking the seed liquid fermented in the step (4) under the aseptic condition, wherein the seed liquid is prepared according to the bacillus licheniformis (B), (C)Bacillus licheniformis ) ACCC 0105030%, Bacillus amyloliquefaciens (B.amyloliquefaciens) (B.amyloliquefaciens)Bacillus amyloliquefaciens) CCTCC NO: m201704025%, Bacillus cereus (B.plantarum) (B.cereus)Bacillus oleronius) CCTCC NO: m201703925%, Pichia pastoris (Pichia farinose) CCTCC NO: m201704420% in proportion, inoculating the mixture into a thallus fermentation culture medium in an inoculation amount of 5%, and carrying out aerobic fermentation at 30-35 ℃ for 24h to prepare a microbial inoculum HQY-of-002.
(7) The preparation method of the thallus fermentation medium used in the step (6) comprises the following steps: 70% of waste water of tail vegetables, 1.0% of molasses, 0.5% of peptone, 0.6% of sodium chloride, 2.0% of dipotassium hydrogen phosphate, 0.2% of magnesium sulfate heptahydrate, and adjusting the pH to about 7.0, and sterilizing the medium at 115 ℃ for 30 minutes.
(8) The microbial inoculum HQY-of-002 obtained by fermentation in the step (6) has the number of viable bacteria>108CFU/mL, can be stably stored for 6 months at room temperature, and the optimal application condition temperature is as follows: 25-45 ℃; pH: 4 to 8.5.
Example 2: preparation of waste water recycling microbial inoculum HQY-of-003 of waste water of waste rape
The microbial inoculum is prepared by the following steps:
(1) taking out each strain preserved in the glycerol freezing tube from a refrigerator at the temperature of minus 80 ℃, melting in an ultra-clean workbench sterilized by ultraviolet, and aseptically sucking 1 ml by a pipette and inoculating into a sterilized 10ml liquid strain activation culture medium for activation.
(2) The preparation method of the liquid strain activation culture medium used in the step (1) for preparing the microbial inoculum comprises the following steps: 1.0 percent of peptone, 0.5 percent of yeast extract, 2.0 percent of glucose, 1.0 percent of sodium chloride, 2.0 per thousand of dipotassium phosphate, 1.0 per thousand of ammonium chloride, 0.2 per thousand of magnesium sulfate heptahydrate and 0.05 per thousand of manganese sulfate, and the pH is adjusted to be about 7.0. And the medium was sterilized at 115 ℃ for 30 minutes.
(3) After the strain in the step (1) of preparing the microbial inoculum is inoculated into an activation culture medium, aerobic activation is carried out for 10-16 h at 30-36 ℃, and then an activation solution can be prepared.
(4) And (3) absorbing the activated strain activating solution in the step (3) under an aseptic condition, inoculating the activated strain activating solution into a seed culture medium by 3 percent of inoculation amount, and carrying out aerobic fermentation at 30-36 ℃ for 12 hours to prepare a seed solution.
(5) The preparation method of the seed culture medium used in the step (4) comprises the following steps: 2.0 percent of peptone, 1.0 percent of glucose, 1.0 percent of molasses, 1.0 percent of sodium chloride, 2.0 thousandth of dipotassium hydrogen phosphate, 2.0 thousandth of ammonium chloride, 0.2 thousandth of magnesium sulfate heptahydrate and 0.05 thousandth of manganese sulfate, and the pH is adjusted to be about 7.0. And the medium was sterilized at 115 ℃ for 30 minutes.
(6) Sucking the seed liquid fermented in the step (4) under the aseptic condition, wherein the seed liquid is prepared according to the bacillus licheniformis (B), (C)Bacillus licheniformis ) ACCC 0105030%, Bacillus amyloliquefaciens (B.amyloliquefaciens) (B.amyloliquefaciens)Bacillus amyloliquefaciens) CCTCC NO: m201704040%, Pichia pastoris (A) ((B))Pichia farinose) CCTCC NO: m201704430%. After mixing, inoculating the mixture into a thallus fermentation culture medium by an inoculation amount of 5%, and carrying out aerobic fermentation at 30-35 ℃ for 24h to prepare a microbial inoculum HQY-of-003.
(7) The preparation method of the thallus fermentation medium used in the step (6) comprises the following steps: 70% of waste water of tail vegetables, 1.0% of molasses, 0.5% of peptone, 0.6% of sodium chloride, 2.0% of dipotassium hydrogen phosphate, 0.2% of magnesium sulfate heptahydrate, and adjusting the pH to about 7.0, and sterilizing the medium at 115 ℃ for 30 minutes.
(8) The microbial inoculum HQY-of-003 of the number of live bacteria after the fermentation in the step (6)>108CFU/mL, can be stably stored for 6 months at room temperature, and the optimal application condition temperature is as follows: 25-45 ℃; pH: 4 to 8.5.
Example 3: preparation of waste water recycling microbial inoculum HQY-of-004 of waste water of Brassica oleracea
The microbial inoculum is prepared by the following steps:
(1) taking out each strain preserved in the glycerol freezing tube from a refrigerator at the temperature of minus 80 ℃, melting in an ultra-clean workbench sterilized by ultraviolet, and aseptically sucking 1 ml by a pipette and inoculating into a sterilized 10ml liquid strain activation culture medium for activation.
(2) The preparation method of the liquid strain activation culture medium used in the step (1) for preparing the microbial inoculum comprises the following steps: 1.0 percent of peptone, 0.5 percent of yeast extract, 2.0 percent of glucose, 1.0 percent of sodium chloride, 2.0 thousandth of dipotassium hydrogen phosphate, 1.0 thousandth of ammonium chloride, 0.2 thousandth of magnesium sulfate heptahydrate and 0.05 thousandth of manganese sulfate, and the pH is adjusted to be about 7.0. And the medium was sterilized at 115 ℃ for 30 minutes.
(3) After the strains in the preparation step (1) of the microbial inoculum are inoculated into an activation culture medium, aerobic activation is carried out for 10-16 h at 30-36 ℃, and then an activation solution can be prepared.
(4) And (3) absorbing the activated strain activating solution in the step (3) under an aseptic condition, inoculating the activated strain activating solution into a seed culture medium by 3 percent of inoculation amount, and carrying out aerobic fermentation at 30-36 ℃ for 12 hours to prepare a seed solution.
(5) The preparation method of the seed culture medium used in the step (4) comprises the following steps: 2.0 percent of peptone, 1.0 percent of glucose, 1.0 percent of molasses, 1.0 percent of sodium chloride, 2.0 thousandth of dipotassium hydrogen phosphate, 2.0 thousandth of ammonium chloride, 0.2 thousandth of magnesium sulfate heptahydrate and 0.05 thousandth of manganese sulfate, and the pH is adjusted to be about 7.0. And the medium was sterilized at 115 ℃ for 30 minutes.
(6) Sucking the seed liquid fermented in the step (4) under the aseptic condition, wherein the seed liquid is prepared according to the bacillus amyloliquefaciens (Bacillus amyloliquefaciens)Bacillus amyloliquefaciens) CCTCC NO: m201704040%, Bacillus botanicus: (B. sp.) (Bacillus oleronius) CCTCC NO: m201703930%, Pichia pastoris (A) ((B))Pichia farinose) CCTCC NO: m201704430%. After mixing, inoculating the mixture into a thallus fermentation culture medium by an inoculation amount of 5%, and carrying out aerobic fermentation at 30-35 ℃ for 24h to prepare a microbial inoculum HQY-of-004.
(7) The preparation method of the thallus fermentation medium used in the step (6) comprises the following steps: 70% of waste water of tail vegetables, 1.0% of molasses, 0.5% of peptone, 0.6% of sodium chloride, 2.0% of dipotassium hydrogen phosphate, 0.2% of magnesium sulfate heptahydrate, and adjusting the pH to about 7.0, and sterilizing the medium at 115 ℃ for 30 minutes.
(8) The microbial inoculum HQY-of-004 fermented in the step (6) has the number of viable bacteria>108CFU/mL, can be stably stored for 6 months at room temperature, and the optimal application condition temperature is as follows: 25-45 ℃; pH: 4 to 8.5.
Example 4:
the process for treating the tail vegetables produced in the vegetable warehouse comprises the following steps: mixed tail vegetable wastewater such as big baby vegetables, tomatoes, beans, cabbage, potatoes, carrots and the like (the COD value of the wastewater is 28500mg/L, the ammonia nitrogen content is 431mg/L, and the total phosphorus content is 119 mg/L), 3 thousandth of microbial inoculum HQY-of-002 is added into the wastewater, the pH value is 35 ℃, the ratio of pH is as follows: 5-8, controlling the dissolved oxygen to be 2-4 mg/L, carrying out aerobic fermentation for 6 days, and diluting pure water to about 100mg/L of COD after fermentation is finished, thus obtaining the plant nutrient solution.
The method comprises the steps of selecting seeds of Sijikuai (Chinese cabbage) of Jiafeng seed Limited company in Jiaxing city, Zhejiang province, selecting full seeds inside, and testing. The test method is operated by referring to the germination index and germination method test of seeds in technical regulations of vegetable waste high-temperature compost innocent treatment (NY/T3441-2019) in agricultural industry standard. And (3) sucking 10ml of nutrient solution into a 9cm culture dish paved with filter paper, uniformly putting 50 seeds into each dish, then putting the culture dish into an incubator at 30 ℃, germinating for 48 hours in a dark place, and counting the germination rate and the root length. Each sample was replicated 3 times with distilled water as a control.
The germination rate of the seeds measured on the 4 th day of germination reaches 100 percent (see figure 1), and the germination index of the seeds reaches 126 percent on the 5 th day of germination (see figure 2-3).
Example 5:
the process for treating the tail vegetables produced in the vegetable warehouse comprises the following steps: mixed tail vegetable wastewater such as big baby vegetables, tomatoes, beans, cabbage, potatoes, carrots and the like (the COD value of the wastewater is 28500mg/L, the ammonia nitrogen content is 431mg/L, and the total phosphorus content is 119 mg/L), 3 thousandth of microbial inoculum HQY-of-003 is added into the wastewater, the pH value is 35 ℃: 5-8, controlling the dissolved oxygen to be 2-4 mg/L, carrying out aerobic fermentation for 6 days, and diluting pure water to about 100mg/L of COD after fermentation is finished, thus obtaining the plant nutrient solution.
The method comprises the steps of selecting seeds of Sijikuai (Chinese cabbage) of Jiafeng seed Limited company in Jiaxing city, Zhejiang province, selecting full seeds inside, and testing. The test method is operated by referring to the germination index and germination method test of seeds in technical regulations of vegetable waste high-temperature compost innocent treatment (NY/T3441-2019) in agricultural industry standard. And (3) sucking 10ml of nutrient solution into a 9cm culture dish paved with filter paper, uniformly placing 50 seeds in each dish, then placing the culture dish into an incubator at 30 ℃, carrying out dark germination for 48 hours, and counting the germination rate and the root length. Each sample was replicated 3 times with distilled water as a control.
The measured seed germination rate reaches 84%, and the seed germination index reaches 92%.
Example 6:
the process for treating the tail vegetables produced in the vegetable warehouse comprises the following steps: mixed tail vegetable wastewater such as big baby vegetables, tomatoes, beans, cabbage, potatoes, carrots and the like (the COD value of the wastewater is 28500mg/L, the ammonia nitrogen content is 431mg/L, and the total phosphorus content is 119 mg/L), 3 thousandth of microbial inoculum HQY-of-004 is added into the wastewater, the pH value is 35 ℃, the ratio of pH is as follows: 5-8, controlling the dissolved oxygen to be 2-4 mg/L, carrying out aerobic fermentation for 6 days, and diluting pure water to about 100mg/L of COD after fermentation is finished, thus obtaining the plant nutrient solution.
The method comprises the steps of selecting seeds of Sijikuai (Chinese cabbage) of Jiafeng seed Limited company in Jiaxing city, Zhejiang province, selecting full seeds inside, and testing. The test method refers to the germination index of seeds in technical specification of harmless treatment of high-temperature compost of vegetable wastes (NY/T3441-2019) in the agricultural industry standard, and the germination test is carried out. And (3) sucking 10ml of nutrient solution into a 9cm culture dish paved with filter paper, uniformly placing 50 seeds in each dish, then placing the culture dish into an incubator at 30 ℃, carrying out dark germination for 48 hours, and counting the germination rate and the root length. Each sample was replicated 3 times with distilled water as a control.
The measured seed germination rate reaches 78%, and the seed germination index reaches 80%.
Example 7:
the process for treating the waste water of the waste vegetables and plants comprises the following steps: mixed wastewater of big baby vegetables, cucumbers, tomatoes, beans, cabbage, potatoes, carrots, aquatic ornamental plants and the like (the COD value of the wastewater is 26300mg/L, the ammonia nitrogen content is 465mg/L, and the total phosphorus is 104 mg/L), 4 thousandth of fungicide HQY-of-002 is added into the wastewater, the pH value is 35 ℃, the pH value is as follows: 5-8, controlling the dissolved oxygen to be 2-4 mg/L, carrying out aerobic fermentation for 6 days, and diluting pure water to about 1000mg/L of COD after fermentation is finished, thus obtaining the plant nutrient solution.
The method comprises the steps of selecting seeds of Sijikuai (Chinese cabbage) of Jiafeng seed Limited company in Jiaxing city, Zhejiang province, selecting full seeds inside, and testing. The test method is operated by referring to the germination index and germination method test of seeds in technical regulations of vegetable waste high-temperature compost innocent treatment (NY/T3441-2019) in agricultural industry standard. And (3) sucking 10ml of nutrient solution into a 9cm culture dish paved with filter paper, uniformly placing 50 seeds in each dish, then placing the culture dish into an incubator at 30 ℃, carrying out dark germination for 48 hours, and counting the germination rate and the root length. Each sample was replicated 3 times with distilled water as a control.
The measured seed germination rate reaches 100%, and the seed germination index reaches 142%.
The microbial inoculum HQY-of-002 contains no pathogenic bacteria, no heavy metal and toxic chemical substances, and the solid product is nontoxic and nonirritating. The microbial inoculum has the advantages of no secondary pollution to the environment in practical application, no additional burden, environment-friendly affinity microbial inoculum product, simple and efficient storage, transportation and delivery of the microbial inoculum. The process is simple to operate, no external chemical is added, and the fermented plant nutrient solution is rich in a large amount of trace elements required by plant growth and has a good effect of promoting the plant growth.
The foregoing description has disclosed fully embodiments of the invention. It should be noted that those skilled in the art can make modifications to the embodiments of the present invention without departing from the scope of the appended claims. Accordingly, the scope of the appended claims is not to be limited to the specific embodiments described above.
Claims (6)
1. A bacteriacide HQY-of-002 for reclaiming the waste water of cabbage is prepared from Bacillus licheniformis (Bacillus licheniformis)Bacillus licheniformis ) ACCC 01050, Bacillus amyloliquefaciens (A)Bacillus amyloliquefaciens) CCTCC NO: m2017040, vegetable bacillus (B.plantarum) (B.plantarum)Bacillus oleronius) CCTCC NO: m2017039 and Pichia pastoris (Pichia farinose) CCTCC NO: m2017044.
2. The tail vegetable wastewater recycling microbial inoculum according to claim 1, which comprises the following components in terms of the number of viable bacteria cultured to the middle and later logarithmic stages: bacillus licheniformis (Bacillus licheniformis ）ACCC 01050 25~35% of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) CCTCC NO: m201704025-35%, and Bacillus cereus (Bacillus cereus)Bacillus oleronius) CCTCC NO: m201703920-30%, Pichia pastoris (A)Pichia farinose）CCTCC NO：M2017044 15%~25%。
3. The waste water recycling microbial inoculum of claim 2, which comprises the following components: bacillus licheniformis (Bacillus licheniformis ) ACCC 0105030%, Bacillus amyloliquefaciens (B.amyloliquefaciens) (B.amyloliquefaciens)Bacillus amyloliquefaciens) CCTCC NO: m201704025%, Bacillus cereus (B.plantarum) (B.cereus)Bacillus oleronius) CCTCC NO: m201703925%, Pichia pastoris (Pichia farinose）CCTCC NO：M2017044 20%。
4. The use of the waste water recycling microbial inoculum of any one of claims 1 to 3 in the preparation of plant nutrient solution by the treatment of plant waste water with recycling microorganisms.
5. The use according to claim 4, wherein the plant wastewater is plant wastewater other than a bactericidal plant.
6. The use according to claim 4, characterized in that the use is: adding the waste water recycle microbial inoculum HQY-of-002 into plant waste water, carrying out aerobic fermentation at 30-37 ℃, controlling dissolved oxygen to be 2-4 mg/L, fermenting for 3-6 days, and diluting to COD (chemical oxygen demand) to be 100-1000 mg/L after the fermentation is finished, thus obtaining the plant nutrient solution.
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