CN114588083A - Large-leaf black tea perfume and preparation method thereof - Google Patents
Large-leaf black tea perfume and preparation method thereof Download PDFInfo
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- CN114588083A CN114588083A CN202210189098.4A CN202210189098A CN114588083A CN 114588083 A CN114588083 A CN 114588083A CN 202210189098 A CN202210189098 A CN 202210189098A CN 114588083 A CN114588083 A CN 114588083A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q13/00—Formulations or additives for perfume preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
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- Birds (AREA)
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- Microbiology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
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- Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Fats And Perfumes (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a large-leaf black tea perfume and a preparation method thereof, wherein the preparation method comprises the following steps: extracting large leaf black tea with water at high temperature, adjusting the temperature of the extract to 40-60 ℃, adding beta-glycosidase and Novoxin compound plant hydrolase ViscozymeL for enzymolysis, heating and refluxing, collecting black tea condensate, mixing with black tea perfume mother liquor, and filtering. In the preparation method of the large-leaf black tea perfume provided by the invention, the beta-glycosidase and the Novoxil compound plant hydrolase ViscozymeL are used for carrying out enzymolysis together, so that the fragrance intensity and the coordination of the obtained extracting solution are obviously improved; and the prepared black tea perfume product has more prominent, soft, harmonious, stable and lasting fragrance by reasonably matching the extracting solution with the monomer compound mother solution and the essential oil mother solution.
Description
Technical Field
The invention belongs to the technical field of tea deep processing, and particularly relates to large-leaf black tea perfume and a preparation method thereof.
Background
The change of aromatic substances in the production of black tea is very complicated, the types and the contents of the aromatic substances in the fresh leaves are usually less, and the types and the contents of the aromatic components are greatly increased after the black tea is prepared. During the black tea processing process, especially in the withering and fermentation stages, a plurality of aroma precursor substances are subjected to corresponding enzymatic conversion to generate a plurality of new aroma components, such as oxidation of alcohols, degradation of amino acids and carotenes, esterification of organic acids and alcohols, oxidative degradation of linolenic acid, isomerization of hexenol, aroma substances such as flowery flavor and fruity flavor are generated, and part of soluble sugar is subjected to caramelization and carbonylamino reaction during the drying process to generate part of aroma substances such as high-boiling caramel aroma, sweet aroma and flower and fruit aroma, so that the tea leaves are converted from green grass aroma to pleasant aroma, and are well favored by wide consumers.
Aromatic substances are extracted from large-leaf black tea, the fragrance is pleasant, and the aromatic substances are determined to be composed of hundreds of volatile substances, mainly comprise linalool, linalool oxide, geraniol, alpha-terpineol, nerol, nerolidol, benzyl alcohol, phenethyl alcohol, alpha-ionone, beta-ionone, geranylacetone, (Z) -jasmone, beta-damascenone, methyl salicylate, phenylacetaldehyde, beta-cyclocitral, hexanoic acid- (E) -2-hexenyl ester, beta-ocimene and other natural fragrance components, and can be used as a substrate of the black tea essence and also used for preparing specific high-end tea perfume.
At present, most of black tea perfume is directly prepared by monomer compounds, and has single fragrance and poor harmony.
In view of this, the invention is particularly proposed.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention provides the large-leaf black tea perfume which is outstanding in fragrance, soft, harmonious, stable and lasting, and the preparation method thereof.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a preparation method of a large-leaf black tea perfume comprises the following steps:
extracting large leaf black tea with water at high temperature, adjusting the temperature of the extract to 40-60 ℃, adding beta-glycosidase and Novoxin compound plant hydrolase ViscozymeL for enzymolysis, heating and refluxing, collecting black tea condensate, mixing with black tea perfume mother liquor, and filtering.
In the technical scheme, the added mass of the beta-glycosidase (the enzyme activity is more than or equal to 50000U/g) is 1.0-1.5 wt% corresponding to 100g of extracting solution.
In the technical scheme, the addition amount of the Novoxin composite plant hydrolase ViscozymeL (KTN02264) with the enzyme activity of 100fbg/g (cellulase activity unit special for Novoxin enzyme preparations) is more than or equal to 2.0 wt% corresponding to 100g of extracting solution.
Further, in the above technical scheme, the enzymolysis specifically comprises:
adding ultrapure water into the large-leaf black tea and water according to the solid-to-liquid ratio of 1 g: 5mL, refluxing and boiling for at least 30min, adding normal-temperature ultrapure water to adjust the temperature of the extracting solution to 50 ℃, adding 1.25 wt% of beta-glycosidase and 2.0 wt% of Novoxin composite plant hydrolase ViscozymeL (KTN02264), and carrying out enzymolysis for 1 h.
Still further, in the above technical means, the amount of the black tea condensate collected is 75 to 85mL for 600g of the powdery raw material of the large leaf black tea.
In a preferred embodiment of the invention the black tea condensate is collected in an amount of 80mL for a powdered feedstock of 600g of large leaf black tea.
Still further, in the above technical solution, the black tea perfume mother liquor comprises monomer compound mother liquor and essential oil mother liquor.
Preferably, in the above technical scheme, the solvent of the mother liquor of black tea perfume is a mixture of dialdehyde ethanol and propylene glycol with the volume ratio of 4: 1.
In detail, in the above technical solution, the effective components in the monomer compound mother liquor include:
linalool, geraniol, phenylacetaldehyde, phenethyl alcohol, alpha-terpineol, methyl salicylate, beta-cyclocitral, nerol, alpha-ionone and beta-ionone.
In detail, in the above technical scheme, the effective components of the essential oil mother liquor comprise:
jasmine essential oil, cedar essential oil, bergamot essential oil and musk essential oil.
In one embodiment of the present invention, the monomer compound mother liquor is prepared by the following steps:
linalool 292.4 μ g, geraniol 189.4 μ g, phenylacetaldehyde 21.2 μ g, phenethyl alcohol 30.6 μ g, alpha-terpineol 3.4 μ g, methyl salicylate 17.4 μ g, beta-cyclocitral 2.3 μ g, nerol 4.1 μ g, alpha-ionone 11.8 μ g and beta-ionone 30.6 μ g are added into 20mL of a mixture of dideal ethanol and propylene glycol with a volume ratio of 4: 1, and the mixture is uniformly mixed to obtain the finished product.
In one embodiment of the present invention, the essential oil mother liquor is prepared by the following steps:
adding 20 μ g of jasmine essential oil, 15 μ g of cedar essential oil, 18 μ g of bergamot essential oil and 7.5 μ g of musk essential oil into 20mL of a mixture of dideal ethanol and propylene glycol with a volume ratio of 4: 1, and mixing uniformly to obtain the final product.
Still further, in the technical scheme, the mixing volume ratio of the black tea condensate to the monomer compound mother liquor to the essential oil mother liquor is 3-8: 1-4.
In a preferred embodiment of the invention, the black tea condensate, the monomeric compound mother liquor and the essential oil mother liquor are mixed in a volume ratio of 5: 2.
The invention also provides the large-leaf black tea perfume prepared by the preparation method.
Compared with the prior art, the invention has the following advantages:
in the preparation method of the large-leaf black tea perfume provided by the invention, the beta-glycosidase and the Novoxil compound plant hydrolase ViscozymeL are used for carrying out enzymolysis together, so that the fragrance intensity and the harmony of the obtained extracting solution are obviously improved; and the prepared black tea perfume product has more prominent, soft, harmonious, stable and lasting fragrance by reasonably matching the extracting solution with the monomer compound mother solution and the essential oil mother solution.
Drawings
FIG. 1 is a graph showing the result of GC-MS analysis of a mixed solution before enzymatic hydrolysis in example 1 of the present invention;
FIG. 2 is a graph showing the result of GC-MS analysis of the mixture after enzymatic hydrolysis in example 1 of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments.
It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
In the examples, the means used are conventional in the art unless otherwise specified.
In the examples, all the starting materials were commercially available products unless otherwise specified.
The terms "comprises," "comprising," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.
In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
Example 1
A preparation method of a large-leaf black tea perfume comprises the following steps:
(1) placing 600g of crushed large-leaf black tea in a 10L extraction bottle, adding ultrapure water according to a solid-liquid ratio of 1g to 5mL (the mass of the tea: the volume of the ultrapure water), heating, refluxing and boiling for 30min, then adding normal-temperature ultrapure water, adjusting the temperature to 50 ℃, then adding 1.25 wt% of beta-glycosidase and 2.0 wt% of Novixin composite plant hydrolase ViscozymeL (KTN02264), performing enzymolysis for 1h, heating and condensing at 100 ℃, and recovering 80mL of black tea condensate;
(2) weighing linalool 292.4 μ g, geraniol 189.4 μ g, phenylacetaldehyde 21.2 μ g, phenethyl alcohol 30.6 μ g, alpha-terpineol 3.4 μ g, methyl salicylate 17.4 μ g, beta-cyclocitral 2.3 μ g, nerol 4.1 μ g, alpha-ionone, 11.8 μ g and beta-ionone 30.6 μ g respectively, dissolving in 20mL of a mixture of dideal ethanol and propylene glycol with a volume ratio of 4: 1 to obtain a monomer compound mother solution for preparing perfume;
(3) respectively weighing 20 mu g of jasmine essential oil, 15 mu g of cedar essential oil, 18 mu g of bergamot essential oil and 7.5 mu g of musk essential oil, and dissolving in 20mL of a mixture of dideal ethanol and propylene glycol with a volume ratio of 4: 1 to obtain an essential oil mother solution for preparing perfume;
(4) dissolving 5mL of black tea condensate, 5mL of monomer compound mother liquor and 2mL of essential oil mother liquor in 50mL of mixed solvent of dialdehyde ethanol and propylene glycol (volume ratio of 4: 1), ultrasonically mixing uniformly, standing at normal temperature for 24h, filtering with 0.45 μm organic filter membrane, and bottling to obtain black tea perfume product (designated as black tea perfume product 1).
Respectively taking 5.0mL before adding glycosidase and Novoxin composite plant hydrolase ViscozymeL (KTN02264) for enzymolysis and after recovering black tea condensate, putting the 5.0mL into a 10mL extraction bottle, adding 1.0g of sodium chloride, adding 2mL of dichloromethane, shaking for 1-2min to fully mix the materials uniformly, standing for 30min, taking out the dichloromethane at the lower layer, adding anhydrous sodium sulfate to absorb water in the dichloromethane, and analyzing by GC-MS.
Specifically, the GC-MS conditions were as follows:
column HP-5MS column (30m × 0.32mm, 0.25 μm);
the temperature of a sample inlet is 250 ℃, and the flow rate of carrier gas (helium, the purity is more than or equal to 99.999%) is 1.0 mL/min;
temperature rising procedure: the initial temperature is 50 ℃, the temperature is kept for 2min, the temperature is increased to 180 ℃ at 3 ℃/min, then the temperature is increased to 250 ℃ at 10 ℃/min, the temperature is kept for 5min, and no shunt sampling is carried out;
an Electron Ionization (EI) source; ionization energy 70 eV; the ion source temperature is 230 ℃; the temperature of a quadrupole rod is 150 ℃, and the emission current is 34.6 muA;
the temperature of the adapter: 280 ℃; the mass scanning range m/z is 35-350, and the solvent delay time is 3 min.
FIGS. 1 and 2 are graphs showing the results of GC-MS analysis of the mixed solution before and after the enzymatic hydrolysis, respectively; the content of the main aroma substances in the obtained mixed solution before and after the enzymolysis is shown in the following table 1.
TABLE 1 content of the main aroma substances before and after the enzymatic hydrolysis
Example 2
A preparation method of a large-leaf black tea perfume comprises the following steps:
(1) placing 600g of pulverized large-leaf black tea in a 10L extraction bottle, adding ultrapure water according to a solid-liquid ratio of 1 g: 5ml (tea leaf mass: ultrapure water volume), heating, refluxing, boiling for 30min, adding room-temperature ultrapure water, adjusting the temperature to 50 ℃, and then adding 0.5 wt% of beta-glucosidase and 2.5 wt% of Novoxin complex plant hydrolase ViscozymeL (KTN02264), 1.5 wt% of beta-glucosidase and 2.0 wt% of Novoxin complex plant hydrolase ViscozymeL (KTN02264), 1.0 wt% of beta-glucosidase and 2.0 wt% of Novoxin complex plant hydrolase ViscozymeL (KTN02264), 0.75 wt% of beta-glucosidase and 2.5 wt% of Novoxin complex plant hydrolase ViscozymeL (KTN02264), 1.5 wt% of Novoxin complex plant hydrolase and 2.5 wt% of Novoxin complex plant hydrolase ViscozymeL (KTN02264), and 2.5 wt% of Novoxin complex plant hydrolase, Carrying out enzymolysis on 0.75 wt% of beta-glycosidase and 1.5 wt% of Novoxin composite plant hydrolase ViscozymeL (KTN02264) for 1h, heating and condensing at 100 ℃, and respectively recovering 80mL of black tea condensate;
(2) weighing linalool 292.4 μ g, geraniol 189.4 μ g, phenylacetaldehyde 21.2 μ g, phenethyl alcohol 30.6 μ g, alpha-terpineol 3.4 μ g, methyl salicylate 17.4 μ g, beta-cyclocitral 2.3 μ g, nerol 4.1 μ g, alpha-ionone, 11.8 μ g and beta-ionone 30.6 μ g respectively, dissolving in 20mL of a mixture of dideal ethanol and propylene glycol with a volume ratio of 4: 1 to obtain a monomer compound mother solution for preparing perfume;
(3) respectively weighing 20 mu g of jasmine essential oil, 15 mu g of cedar essential oil, 18 mu g of bergamot essential oil and 7.5 mu g of musk essential oil, dissolving in 20mL of a mixture of dideal ethanol and propylene glycol with the volume ratio of 4: 1 to obtain an essential oil mother solution for preparing perfume;
(4) dissolving 5mL of black tea condensate, 5mL of monomer compound mother liquor and 2mL of essential oil mother liquor in 50mL of mixed solvent of dialdehyde ethanol and propylene glycol (volume ratio of 4: 1), ultrasonically mixing uniformly, standing at normal temperature for 24h, filtering with 0.45 μm organic filter membrane, and bottling to obtain black tea perfume product (sequentially counted as black tea perfume product 2-8).
Similarly to example 1, 5.0mL of each of the collected black tea condensates were analyzed by GC-MS to find the contents of the main aroma substances in the mixed liquid, and the results are shown in Table 2 below.
TABLE 2 increase ratio (unit%) of main aroma after enzymolysis
Example 3
A preparation method of a large-leaf black tea perfume comprises the following steps:
(1) placing 600g of crushed large-leaf black tea in a 10L extraction bottle, adding ultrapure water according to a solid-liquid ratio of 1g to 5mL (the mass of the tea: the volume of the ultrapure water), heating, refluxing and boiling for 30min, then adding normal-temperature ultrapure water, adjusting the temperature to 50 ℃, then adding 1.25 wt% of beta-glycosidase and 2.0 wt% of Novixin composite plant hydrolase ViscozymeL (KTN02264), performing enzymolysis for 1h, heating and condensing at 100 ℃, and respectively recovering 60mL, 70mL, 90mL and 100mL of black tea condensate;
(2) weighing linalool 292.4 μ g, geraniol 189.4 μ g, phenylacetaldehyde 21.2 μ g, phenethyl alcohol 30.6 μ g, alpha-terpineol 3.4 μ g, methyl salicylate 17.4 μ g, beta-cyclocitral 2.3 μ g, nerol 4.1 μ g, alpha-ionone, 11.8 μ g and beta-ionone 30.6 μ g respectively, dissolving in 20mL of a mixture of dideal ethanol and propylene glycol with a volume ratio of 4: 1 to obtain a monomer compound mother solution for preparing perfume;
(3) respectively weighing 20 mu g of jasmine essential oil, 15 mu g of cedar essential oil, 18 mu g of bergamot essential oil and 7.5 mu g of musk essential oil, dissolving in 20mL of a mixture of dideal ethanol and propylene glycol with the volume ratio of 4: 1 to obtain an essential oil mother solution for preparing perfume;
(4) dissolving 5mL of black tea condensate, 5mL of monomer compound mother liquor and 2mL of essential oil mother liquor in 50mL of mixed solvent of dialdehyde ethanol and propylene glycol (volume ratio of 4: 1), ultrasonically mixing uniformly, standing at normal temperature for 24h, filtering with 0.45 μm organic filter membrane, and bottling to obtain black tea perfume product (sequentially counted as black tea perfume product 9-12).
Similarly to example 1, the obtained black tea condensate was separately recovered, and the content of the main aroma substances in the mixed liquid thereof was analyzed by GC-MS, and the results are shown in table 3 below.
TABLE 3 increase ratio (%)
In addition, the prepared black tea perfume products 1 to 12 were taken and evaluated for the intensity of fragrance and the harmony of fragrance, and the results are shown in table 4 below.
TABLE 4 Scoring of fragrance intensity and fragrance compatibility of Black tea perfume products
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention.
It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. The preparation method of the large-leaf black tea perfume is characterized by comprising the following steps of:
extracting large leaf black tea with water at high temperature, adjusting the temperature of the extract to 40-60 ℃, adding beta-glycosidase and Novoxin compound plant hydrolase ViscozymeL for enzymolysis, heating and refluxing, collecting black tea condensate, mixing with black tea perfume mother liquor, and filtering.
2. The method for preparing a large leaf black tea perfume according to claim 1,
the addition amount of the beta-glycosidase is 1.0 to 1.5 percent by weight;
and/or the addition amount of the Novoxil compound plant hydrolase ViscozymeL is more than or equal to 2.0 wt%.
3. The process for producing a large leaf black tea perfume according to claim 1 or 2,
the enzymolysis specifically comprises the following steps:
adding ultrapure water into black tea of large leaf variety and water at a solid-to-liquid ratio of 1 g: 5mL, reflux boiling for at least 30min, adding ultrapure water at normal temperature to adjust the temperature of the extractive solution to 50 deg.C, adding 1.25 wt% beta-glycosidase and 2.0 wt% Novoxin composite plant hydrolase ViscozymeL, and performing enzymolysis for 1 h.
4. A process for preparing a large leaf black tea perfume according to any one of claims 1 to 3,
the black tea condensate is collected in an amount of 75-85mL, preferably 80mL, corresponding to 600g of powdered raw material of large leaf black tea.
5. The process for producing a large leaf black tea perfume according to any one of claims 1 to 4,
the black tea perfume mother liquor comprises monomer compound mother liquor and essential oil mother liquor;
preferably, the solvent of the black tea perfume mother liquor is a mixture of dealdehydic ethanol and propylene glycol with the volume ratio of 4: 1.
6. The method for preparing a large leaf black tea perfume according to claim 5,
the effective components in the monomer compound mother liquor comprise:
linalool, geraniol, phenylacetaldehyde, phenethyl alcohol, alpha-terpineol, methyl salicylate, beta-cyclocitral, nerol, alpha-ionone, beta-ionone;
and/or the effective components of the essential oil mother liquor comprise:
jasmine essential oil, cedar essential oil, bergamot essential oil and musk essential oil.
7. The method for preparing a large leaf black tea perfume according to claim 6,
the preparation method of the monomer compound mother liquor comprises the following steps:
linalool 292.4 μ g, geraniol 189.4 μ g, phenylacetaldehyde 21.2 μ g, phenethyl alcohol 30.6 μ g, alpha-terpineol 3.4 μ g, methyl salicylate 17.4 μ g, beta-cyclocitral 2.3 μ g, nerol 4.1 μ g, alpha-ionone 11.8 μ g and beta-ionone 30.6 μ g are added into 20mL of a mixture of dideal ethanol and propylene glycol with a volume ratio of 4: 1, and the mixture is uniformly mixed to obtain the finished product.
8. The method for preparing a large leaf black tea perfume according to claim 6,
the preparation method of the essential oil mother liquor comprises the following steps:
adding 20 μ g of jasmine essential oil, 15 μ g of cedar essential oil, 18 μ g of bergamot essential oil and 7.5 μ g of musk essential oil into 20mL of a mixture of dialdehyde ethanol and propylene glycol with a volume ratio of 4: 1, and mixing uniformly to obtain the product.
9. A process for preparing a large leaf black tea perfume according to any one of claims 5 to 8,
the mixing volume ratio of the black tea condensate to the monomer compound mother liquor to the essential oil mother liquor is 3-8: 1-4;
preferably, the mixing volume ratio of the black tea condensate, the monomer compound mother liquor and the essential oil mother liquor is 5: 2.
10. A black tea perfume of large leaf species prepared by the process according to any one of claims 1 to 9.
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