CN114586560A - Method for measuring drought stress degree of pitaya - Google Patents
Method for measuring drought stress degree of pitaya Download PDFInfo
- Publication number
- CN114586560A CN114586560A CN202210436361.5A CN202210436361A CN114586560A CN 114586560 A CN114586560 A CN 114586560A CN 202210436361 A CN202210436361 A CN 202210436361A CN 114586560 A CN114586560 A CN 114586560A
- Authority
- CN
- China
- Prior art keywords
- degree
- drought
- dragon fruit
- fleshy
- fiber bundles
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention provides a method for measuring drought stress degree of pitaya, and relates to the technical field of plant morphological dissection. The measurement method provided by the invention is simple and easy to implement, has higher result accuracy and has important significance for analyzing the drought resistance mechanism of plants, and the drought stress degree endured by the pitaya is determined by observing and measuring morphological indexes and anatomical indexes of drought-treated and normally-supplied pitaya samples.
Description
Technical Field
The invention relates to the technical field of plant morphological dissection, in particular to a method for measuring drought stress degree of dragon fruits.
Background
Pitaya (Hylocereus polyrhizus) is a cultivation plant for fruits of Cactaceae (Cactaceae) genus (Hylocereus), the fruits have unique appearance, bright color and rich nutrition, and good economic, ecological and social benefits, and the planting area of the Pitaya is rapidly enlarged in many tropical and subtropical regions around the world. The water requirement of the dragon fruits is high in the fruit expansion period, and drought is a main factor influencing the yield of the dragon fruits, so that the measurement of the drought stress degree of the dragon fruits has important guiding reference significance for guiding watering and irrigation of the dragon fruits and high and stable yield.
At present, the drought stress analysis of the dragon fruit mainly relates to the determination of physiological indexes. The leaves are the first reaction organs of plants responding to drought stress, and the wilting degree of the leaves of the plants under the drought stress is obviously related to the drought stress tolerance degree of the leaves. However, the leaves of the dragon fruit are degenerated into thorns, the change of the thorns is not influenced by drought stress, and the stress degree of the dragon fruit under the drought stress is not easy to observe. Therefore, by researching the relationship between the shape and anatomical structure of the dragon fruit and the drought resistance, the method is beneficial to accurately measuring the drought stress degree of the dragon fruit and establishing an experimental model for researching the drought resistance molecular mechanism of the dragon fruit.
Disclosure of Invention
The invention aims to provide a method for measuring the drought stress degree of dragon fruits, which can measure the drought stress degree of the dragon fruits through morphological and anatomical observation, is simple and easy to implement, and has more accurate judgment result.
In order to achieve the above object, the present invention provides the following technical solutions:
a method for measuring drought stress degree of dragon fruit comprises determining drought stress degree of dragon fruit according to morphological index and anatomical index of dragon fruit; the morphological indexes comprise ridge thickness, ridge color and wilting degree of fleshy stems; the anatomical indicators include the degree of aggregation of the fiber bundle and the size of cells surrounding the fiber bundle.
In the invention, the morphological index observation object is a tender prismatic sheet with thorn seats on the fleshy stem at the overground part of the dragon fruit seedling.
In the invention, the observation objects of the thickness and the color of the edge are barbed edge pieces from the top to the lower 1/3 position of the dragon fruit seedling.
In the present invention, the wilting degree of the fleshy stem refers to the ratio of the length of the fleshy stem wilting from the top to the length of the whole overground part.
In the invention, the anatomical index observation object is a fleshy stem from the top to the lower 1/3 position of the dragon fruit seedling.
In the present invention, the degree of aggregation of the fiber bundles refers to the degree of decrease in the average distance of the fiber bundles from each other, as compared to normally watered pitaya.
In the invention, the drought stress degree of the pitaya comprises mild drought, moderate drought and severe drought.
In the invention, the mild drought judgment standard is that the wilting degree of fleshy stems does not exceed 1/4, compared with normal watering, the color of the edge pieces from the top to the bottom 1/3 of the dragon fruit seedlings is light green, the thickness of the edge pieces is not obviously changed, and the size of cells around fiber bundles of the fleshy stems and the aggregation degree of the fiber bundles are not obviously changed; the medium drought judgment standard is that the wilting degree of fleshy stems is about 1/2, compared with normal watering, the color of the edge pieces from the top to the bottom 1/3 of the dragon fruit seedlings is green, the thickness of the edge is obviously thinned, fiber bundles of fleshy stems are gathered, and cells around the fiber bundles become small; the severe drought standard is that the wilting degree of fleshy stems is expanded to 3/4 or above, compared with normal watering, the color of the edge pieces from the top to the bottom 1/3 of the dragon fruit seedlings is green and wrinkled, the edge pieces are hollow, the fiber bundles of the fleshy stems are gathered together, and the cells around the fiber bundles are reduced or broken.
The invention also provides an application of the method for measuring the drought stress degree of the succulent plant.
In the present invention, the succulent plant is a cactaceae plant.
Compared with the prior art, the invention has the following beneficial effects:
the method adopts morphological indexes including the thickness of the edges, the color of the fleshy stems and the wilting degree of the fleshy stems and anatomical indexes including the aggregation degree of fiber bundles and the sizes of cells around the fiber bundles to determine the drought stress degree of the dragon fruits, has simple operation, and does not need complicated experimental means and expensive reagents for physiological and biochemical detection. The measuring method provided by the invention is based on the form and combines anatomical observation to judge the stress degree of the dragon fruit seedlings, so that the result is more accurate. The invention provides reference for excavating and identifying drought-resistant genes and key metabolic pathways, for early sensing of plant drought-resistant signals and starting drought-resistant measures, and has important theoretical significance for crop drought-resistant genetic improvement.
Drawings
Fig. 1 is a morphological anatomical feature diagram of dragon fruit.
Detailed Description
A method for measuring drought stress degree of dragon fruit comprises determining drought stress degree of dragon fruit according to morphological index and anatomical index of dragon fruit; the morphological indexes comprise ridge thickness, ridge color and wilting degree of fleshy stems; the anatomical indicators include the degree of aggregation of the fiber bundle and the size of cells surrounding the fiber bundle. In the invention, the dragon fruit plant seedlings are planted, a drought stress group and a control group are set, and the drought stress degree of the dragon fruits is determined by measuring morphological indexes and anatomical indexes of two groups of plants treated in the same time period. Wherein, the drought stress group is to plant the dragon fruit seedlings in plastic pots, place the plastic pots in a light culture room for culture and consume water naturally; the control group was a contemporaneous pitaya seedling with normal water supply. In the present invention, the culture conditions in the light-irradiation culture chamber are that the light irradiation is performed for 12 to 16 hours per day and the light flux density is 200 to 400. mu. mol. m-2·s-1The day and night constant temperature is 22-26 ℃ and the relative humidity is 55-65%, but the invention is not limited to the protection scope.
In the present invention, the anatomical index is obtained by a method including, but not limited to, a frozen section method and a paraffin section method. In the present invention, as one of the modes that can be carried out, a tissue section of a stem of a pitaya is obtained by a cryo-sectioning method, and is placed under an optical microscope (CX41RF, Olympus Corporation, Tokyo, Japan) to observe photographing and micro-measure an anatomical index.
In the invention, the morphological index observation object is a tender prismatic sheet with thorn seats on the fleshy stem at the overground part of the dragon fruit seedling.
In the present invention, the observation object of the thickness and color of the edge is the barbed edge piece from the top to the bottom 1/3 of the dragon fruit seedling.
In the present invention, the wilting degree of the fleshy stem refers to the ratio of the length of the fleshy stem wilting from the top to the length of the whole overground part. In the invention, the wilting degree of the fleshy stem can be reflected by measuring the ratio because the dragon fruit has the characteristic that the water is transferred from the top of the fleshy stem to the bottom and the root system. In the invention, the wilting degree of the fleshy stem is measured by a ruler.
In the present invention, the anatomical index observation object is the fleshy stem from the top of the dragon fruit seedling to 1/3 below.
In the present invention, the degree of aggregation of the fiber bundles refers to the degree of decrease in the average distance of the fiber bundles from each other, as compared to normally watered pitaya. In the present invention, the degree of aggregation of the fiber bundle is measured by microscopic measurement methods well known to those skilled in the art.
In the invention, the drought stress degree of the pitaya comprises mild drought, moderate drought and severe drought. In the invention, the mild drought judgment standard is that the wilting degree of fleshy stems does not exceed 1/4, and compared with normal watering, the color of the edge pieces from the top to the bottom 1/3 of the dragon fruit seedlings is light green, the thickness of the edge pieces is not obviously changed, and the size of cells around fiber bundles of the fleshy stems and the aggregation degree of the fiber bundles are not obviously changed; the medium drought judgment standard is that the wilting degree of fleshy stems is about 1/2, compared with normal watering, the color of the edge pieces from the top to the bottom 1/3 of the dragon fruit seedlings is green, the thickness of the edge is obviously thinned, fiber bundles of fleshy stems are gathered, and cells around the fiber bundles become small; the severe drought standard is that the wilting degree of fleshy stems is expanded to 3/4 or above, compared with normal watering, the color of the edge pieces from the top to the bottom 1/3 of the dragon fruit seedlings is green and wrinkled, the edge pieces are hollow, the fiber bundles of the fleshy stems are gathered together, and the cells around the fiber bundles are reduced or broken.
The invention provides an application of the method for measuring the drought stress degree of succulent plants. In the present invention, the succulent plant is the family Cactaceae, which includes, but is not limited to, the genera Gastrodia and Ceratopsis.
The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The test adopts a random block design, and a control group and a drought group are set, wherein each group comprises 10 pots and 5 plants. And (5) watering thoroughly before the test to ensure that the water content of the soil in each pot reaches a saturated state.
And (3) treatment: watering the control group for 1 time every 3 days to ensure that the relative water content of the soil is between 75% and 80%, continuously consuming water for 30 days in the drought group, observing the thickness and the color of the overground parts of the edges of the seedlings of the dragon fruits in the control group and the drought group at 10, 20 and 30 days, and measuring the wilting degree of the fleshy stems. Meanwhile, the aggregation degree of the fiber bundles and the cell diameter around the fiber bundles from the top of the fleshy stem of the dragon fruit in the control group and the drought group to 1/3 below were measured microscopically.
The measuring method comprises the following steps: the wilting degree of fleshy stems is measured by a ruler, and the anatomic observation method is a frozen section method. Wherein the frozen section method comprises the following steps: pitaya stem tissue was embedded using OCT embedding medium (Catalog number 4583, Skura, USA) on an embedding block, and after the OCT embedding medium was cryosolidified, the embedding block was fixed on a Leica CM1950 type cryomicrotome (CM1950, Leica biosystems Nussoch GmbH, Nussoch, Germany) to perform sectioning, the tissue block was roughly trimmed before sectioning to a slice thickness of about 10 μm, and the sections were then placed under an optical microscope for observation and microscopic measurement.
Morphological structure result analysis:
during the whole treatment process, the edges of the dragon fruit seedlings in the control group from the top to the 1/3 are light green and full, and no obvious wilting character appears (fig. 1 a). Under drought stress, the top of the fleshy stem of dragon fruit begins to lose water and wilting, and as the duration of the stress is prolonged, the fleshy stem gradually loses water and wilts from top to bottom. The drought stress lasts for 10 days, the color of the thorn-carrying edge pieces from the top to 1/3 of the dragon fruit seedlings in the drought group is still green, the edge thickness is not obviously changed, and the wilting degree of fleshy stems is less than 1/4 (figure 1 b); the drought stress lasts for 20 days, the thorn-carrying prism sheets from the top to 1/3 of the dragon fruit seedlings in the drought group become thin due to water loss, the color is green, and the wilting degree of fleshy stems is about 1/2 (figure 1 c); the drought stress lasts for 30 days, and the thorn-carrying prism sheets from the top to the lower 1/3 of the dragon fruit seedlings in the drought group become hollow due to severe water loss, have green and wrinkled color and have wilting degree of fleshy stems more than 3/4 (fig. 1 d).
TABLE 1 Pitaya seedling morphology Change under continuous drought stress
Analysis of anatomical results:
under normal water supply conditions, the fiber bundles in the cross section from the top to 1/3 lower part of the fleshy stem of the dragon fruit seedlings in the control group are arranged regularly, the interval between the fiber bundles is large, and the cells around the fiber bundles are complete (fig. 1 a'). The drought stress lasted 10 days, and the degree of fiber bundle aggregation and surrounding cell diameter at 1/3 from the top of the dragon fruit seedlings in the drought group did not significantly change compared to the control (fig. 1 b'); the drought stress lasted 20 days, and the fiber bundles at 1/3 from the top of the drought group of dragon fruit seedlings became smaller in distance from each other and their surrounding cell diameters became smaller (fig. 1 c'); drought stress lasted 30 days, and fiber bundles at the top to the bottom 1/3 of the drought group of dragon fruit seedlings aggregated together with their surrounding cells becoming smaller in diameter and ruptured (fig. 1 d').
TABLE 2 anatomical changes in fleshy stems of pitaya under continuous drought stress
The judging method comprises the following steps:
under the condition of normal water supply, the edges from the top to the lower 1/3 position of the dragon fruit seedlings in the control group are all bright green and full, no obvious wilting character appears, the fiber bundles in the cross section are arranged regularly, the intervals among the fiber bundles are large, and the cells around the fiber bundles are complete. Under drought stress, the top of the fleshy stem loses water and wilts firstly, the wilting degree of the fleshy stem does not exceed 1/4, compared with a control, the color of the edge pieces from the top to the bottom 1/3 of the dragon fruit seedlings is light green, the thickness of the edge is not obviously changed, the size of cells around fiber bundles of the fleshy stem and the aggregation degree of the fiber bundles are not obviously changed, and the dragon fruit seedlings are judged to be slightly drought; the wilting degree of the fleshy stems is about 1/2, compared with a control, the color of the edge pieces from the top to the bottom 1/3 of the dragon fruit seedlings is green, the thickness of the edge pieces is obviously thinned, the fiber bundles of the fleshy stems are gathered, and the cells around the fiber bundles are reduced, so that the moderate drought is judged; the wilting degree of the fleshy stems is expanded to 3/4 or above, compared with a control, the color of the edge pieces from the top to the bottom 1/3 of the dragon fruit seedlings is stained with green folds, the edge pieces are hollow, the fiber bundles of the fleshy stems are gathered together, and the cells around the fiber bundles are reduced or broken, so that the severe drought is judged.
TABLE 3 determination method of drought stress degree of dragon fruit seedlings
In conclusion, the drought stress degree of the dragon fruit can be measured through morphological and anatomical observation, the method is simple and easy to implement, and the result is more accurate.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and it will be apparent to those skilled in the art that several modifications and equivalents can be made within the spirit and principle of the present invention, and these modifications and equivalents should also be regarded as the scope of the present invention.
Claims (10)
1. A method for measuring the drought stress degree of the dragon fruit is characterized in that the drought stress degree of the dragon fruit is determined by morphological indexes and anatomical indexes of the dragon fruit; the morphological indexes comprise ridge thickness, ridge color and wilting degree of fleshy stems; the anatomical indicators include the degree of aggregation of the fiber bundle and the size of cells surrounding the fiber bundle.
2. The method according to claim 1, wherein the morphology index observation object is a tender prism sheet with thorn-shaped upper fleshy stem of the dragon fruit seedling.
3. The method of claim 1, wherein the observation of the thickness and color of the ribs is performed by a barbed rib at 1/3 from the top of the dragon fruit seedling.
4. The method of claim 1, wherein the degree of fleshy stem wilting is the ratio of the length of fleshy stem wilting from top to bottom to the length of the whole overground part.
5. The method of claim 1, wherein the anatomical target observation is a fleshy stem from the top of a dragon fruit seedling to 1/3 below.
6. The method of claim 1, wherein the degree of fiber strand aggregation is a reduction in the average distance of the fiber strands from each other as compared to a normally watered dragon fruit.
7. The method of claim 1, wherein the degree of drought stress on the pitaya comprises mild drought, moderate drought, and severe drought.
8. The method according to claim 7, wherein the mild drought judgment standard is that the wilting degree of fleshy stems does not exceed 1/4, and compared with normal watering, the color of the edge pieces from the top to the bottom 1/3 of the dragon fruit seedlings is light green, the thickness of the edge pieces is not significantly changed, and the size of cells around the fiber bundles of the fleshy stems and the aggregation degree of the fiber bundles are not significantly changed; the medium drought judgment standard is that the wilting degree of fleshy stems is about 1/2, compared with normal watering, the color of the edge pieces from the top to the bottom 1/3 of the dragon fruit seedlings is green, the thickness of the edge is obviously thinned, fiber bundles of fleshy stems are gathered, and cells around the fiber bundles become small; the severe drought standard is that the wilting degree of fleshy stems is expanded to 3/4 or above, compared with normal watering, the color of the edge pieces from the top to the bottom 1/3 of the dragon fruit seedlings is green and wrinkled, the edge pieces are hollow, the fiber bundles of the fleshy stems are gathered together, and the cells around the fiber bundles are reduced or broken.
9. Use of a method according to any one of claims 1 to 8 to measure the drought stress level of succulent plants.
10. Use according to claim 9, wherein the succulent plant is a cactaceae plant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210436361.5A CN114586560A (en) | 2022-04-25 | 2022-04-25 | Method for measuring drought stress degree of pitaya |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210436361.5A CN114586560A (en) | 2022-04-25 | 2022-04-25 | Method for measuring drought stress degree of pitaya |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114586560A true CN114586560A (en) | 2022-06-07 |
Family
ID=81813161
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210436361.5A Pending CN114586560A (en) | 2022-04-25 | 2022-04-25 | Method for measuring drought stress degree of pitaya |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114586560A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112330694A (en) * | 2020-11-16 | 2021-02-05 | 新疆农业科学院粮食作物研究所 | Plant wilting degree calculation method and system |
-
2022
- 2022-04-25 CN CN202210436361.5A patent/CN114586560A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112330694A (en) * | 2020-11-16 | 2021-02-05 | 新疆农业科学院粮食作物研究所 | Plant wilting degree calculation method and system |
Non-Patent Citations (2)
| Title |
|---|
| 张依楠等: "水分胁迫对火龙果茎解剖结构的影响", 《山地农业生物学报》 * |
| 齐钊等: "干旱胁迫对火龙果植株形态及生理指标的影响", 《广东农业科学》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Mäkinen et al. | Seasonal changes in stem radius and production of new tracheids in Norway spruce | |
| CN106446596B (en) | To the significant relevant molecular labeling of waterlogging tolerance of chrysanthemum and its identification method and application | |
| Malone et al. | The spittlebug Philaenus spumarius feeds from mature xylem at the full hydraulic tension of the transpiration stream | |
| CN101513167A (en) | Method for obtaining a distant hybrid between cultivating chrysanthemum and related genera | |
| CN105248272A (en) | Breeding method of high-photosynthetic-efficiency core collections of rape | |
| Dörken et al. | Anatomical aspects of xeromorphy in arid-adapted plants of Australia | |
| CN110249836B (en) | Method for identifying cold resistance of muskmelon at seedling stage | |
| CN103837534B (en) | A kind of method measuring paenoiae alba Pollen Activity | |
| CN109952907B (en) | Method for screening recovery species of tropical coral island vegetation based on functional characters | |
| Smith et al. | A textbook of general botany | |
| CN103115924B (en) | The determination methods of age of rattan material | |
| CN108651273B (en) | Method for breeding salt-tolerant lagerstroemia indica leaves by polymerization based on gene detection | |
| CN114586560A (en) | Method for measuring drought stress degree of pitaya | |
| Bailey | Anomalous growth and vegetative anatomy of Simmondsia chinensis | |
| CN102768210B (en) | Identification method of interspecific hybrids of Brassica campestris and Brassica napus | |
| Mehta et al. | Trichome morphology as an indicator of high rubber bearing guayule (Parthenium argentatum Gray) plants in native populations | |
| CN105602948A (en) | Genes and method for identifying gossypium hirsutum linn. variety verticillium wilt resistance by fluorescence quantitative PCR technique | |
| CN115266617A (en) | Screening method of low-temperature-resistant garden chrysanthemum varieties in plateau area | |
| Ehara | Comparative Morphological Studies On The Hop (Humulus Lupulus L.) And The Japanese Hop (H. Japonicus Sieb. ET ZUCC.). Ⅰ | |
| Piehl | The natural history and taxonomy of Comandra (Santalaceae) | |
| Butare | Effect of temperature on plant growth and yield in everbearing strawberry Fragaria x ananassa, cv. Florentina | |
| Fjell | Preformation of root primordia in shoots and root morphogenesis in Salix viminalis | |
| CN102007845A (en) | Application of top-grafting and head-exchanging grafting method to cultivation of ligustrum japonicum 'howardii' | |
| Thoday et al. | Studies in Growth and Differentiation: II. A Preliminary Survey of the Morphology and Anatomy of Kleinia articulata, Haw | |
| Wardlaw et al. | Experimental and Analytical Studies of Pteridophytes: XXXIX. Morphogenetic Investigations of Sori in Leptosporangiate Ferns |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |