CN1145416C - Extracting process for seaweed plant growth hormone - Google Patents
Extracting process for seaweed plant growth hormone Download PDFInfo
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- CN1145416C CN1145416C CNB011351373A CN01135137A CN1145416C CN 1145416 C CN1145416 C CN 1145416C CN B011351373 A CNB011351373 A CN B011351373A CN 01135137 A CN01135137 A CN 01135137A CN 1145416 C CN1145416 C CN 1145416C
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- seaweed
- plant growth
- growth hormone
- ethanol
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Abstract
The present invention relates to a process for extracting seaweed plant growth hormone, which belongs to the preparation of seaweed biochemical substances, and is used for extracting betaine and the analogues from seaweed. The present invention provides the technical scheme on the basis of previous theoretical researches, and the process is characterized in that the process comprises six procedures of flushing fresh seaweed and cutting up the seaweed after removing foreign substances, adding anhydrous ethanol into the matters until the concentration of ethanol is 65 to 75%, obtaining crude extract after beating, extraction, water, ethanol and pigment removal by evaporation and salt separation by centrifugation, etc. additionally, the present invention has the advantages of simple process, low extraction cost, comprehensive development of abundant seaweed resources, reinforcement and development to the use of natural fertilizers, increased crop yield and quality, etc.
Description
Technical field
The invention discloses the extraction process of a kind of seaweed plant growth hormone (betain and analog thereof), the preparing technical field that belongs to the phycological biochemistry material is used for extracting the amino acid of the natural fertilizers additive that can be used as promotion crop growth raising output or the derivative of imino acid from marine alga.
Background technology
In the prior art, people recognize for a long time that through research betain is the derivative of class of amino acid or imino acid, contain the quinquevalent nitrogen of exhaustive methylation, exist with the version of open loop and closed loop.And know that for a long time this material can be used as the growth stimulator of plant.As just once thinking that the glycinebetaine (quaternary ammonium compound) in the beet was a kind of promoting growth of plants conditioning agent and the class cytokinin activity with higher level as far back as Wheeler in 1962.A large amount of research simultaneously both at home and abroad also confirms: betain in the agricultural marine algae extract and cytokinin are the active substances of main promotion plant growing, and find that very the betain of low concentration just can improve the plant content of chlorophyll greatly, and have antimycotic and improve effect such as crop yield.But not seeing up to now has the technology of extracting betain and analog (comprising quaternary ammonium compound and uncle's sulphur compound) thereof from marine alga, particularly can be used for the technology of suitability for industrialized production.Ministry of Education's Help Center's update search in trust is located at Qingdao Marine University is also found out and is not still had at present and the similar technology of the present invention.
Summary of the invention
In order to overcome the research that only rests in the prior art contained betain in the marine alga and analog version, characteristic and function, and lack the particularly deficiency of the production technology extracted of heavy industrialization of extraction process, the invention provides and a kind ofly can carry out suitability for industrialized production, again can comprehensive development and utilization China rich seaweed resources very, strengthen the agricultural effect of increasing production of marine alga natural fertilizer, method is simple, the extraction process of the seaweed plant growth hormone that extraction cost is low.
The technical solution adopted for the present invention to solve the technical problems, it is the extraction process of seaweed plant growth hormone of the present invention, it is characterized in that this technology comprises the following steps: that 1. remove impurity and exotic with fresh marine alga with fresh water flushing, and clean marine alga is shredded with cutter mill; 2. adding absolute ethyl alcohol to concentration of alcohol in the marine alga of having shredded is 65～75%, and with the beater making beating, gets filtrate through suction filtration; 3. after extracting 22～26 hours, make extract; 4. remove moisture, ethanol and pigment with the rotary evaporation method and get concentrate; 5. concentrate is carried out centrifugal treating, remove the salinity that separates out, make the crude extract of clarification; 6. in crude extract, add excessive Reinecke's salt (four thiocyanogens, two ammino chromium propylhomoserins, ammonium reinecke, ammonium tetrathiocyanat-odiamminechromate) to form Reinecke's acid quaternary ammonium compound precipitation (comprising betain and derivative thereof), after centrifugal, promptly gets product of the present invention again.
In order to improve extraction yield, above-mentioned step 2 makes that also available ethanol merges in the filtrate to filter residue flushing 1～2 time and with washing lotion in the process of filtrate.
For plant growth hormone contained in the marine alga is all precipitated, above-mentioned step 6 should be controlled at precipitation temperature under-1 ℃～-3 ℃ conditions carries out.
The invention has the beneficial effects as follows: the present invention is the application and the development of country " 95 " brainstorm subject " research of novel alga plant growth substance " scientific achievement that the inventor is born, it is to strengthening and promoting using and improving its quality and will play facilitation with the quality and yield that improves crops itself etc. of China's natural fertilizers, this technology not only is suitable for suitability for industrialized production, can fully develop the marine algae resource of China's abundant on a large scale, promote the development of natural seaweed fertilizer, and it is simple for process, extraction cost is low, especially in the enterprise that produces seaweed products, implement, go all out with the comprehensive utilization of marine alga, more progressive reducing production costs.
Embodiment
One of most preferred embodiment of the present invention is: get 100 kilograms in the fresh sea-tangle propagated artificially, remove impurity and exotic, with cutter mill clean sea-tangle shred then with the fresh water flushing, the about 40kg of adding absolute ethyl alcohol to concentration of alcohol be 70%; With beater frond is broken into slurry again, filter residue also stirs extraction 24 hours with the alcohol flushing twice of 2kg70%, merging filtrate; Remove water, ethanol and pigment (pigment generally is deposited on the glass wall) etc. for 60 ℃ with large-scale evaporimeter; As seen the salinity that separate out in a large number this moment is removed these salinities with suction method again, makes the crude extract of clarification.To place temperature be-2 ℃ ice-water bath and gently add excessive Reinecke's salt solution in the container until precipitation fully till the generation with the container that fills this crude extract.Promptly get product plant growth hormone 4.6kg of the present invention after the filtration.
Two of embodiment be the absolute ethyl alcohol that adds in one of most preferred embodiment with above-mentioned change into about 35kg to concentration of alcohol be 65%, filter residue is washed twice with the ethanol of 2.5kg70%, extracted 26 hours, it is-1 ℃ ice-water bath that the container that fills crude extract is placed temperature, other conditions and step are identical with one of above-mentioned most preferred embodiment fully, make product 4.2kg of the present invention.
Three of embodiment is: with the absolute ethyl alcohol that adds in one of above-mentioned most preferred embodiment change into about 45kg to concentration of alcohol be 75%, filter residue is washed once with the ethanol of 2.5kg70%, extracted 22 hours, it is-3 ℃ ice-water bath that the container that fills crude extract is placed temperature, other conditions and step are identical with one of above-mentioned most preferred embodiment fully, make product 5kg of the present invention
Claims (2)
1, a kind of extraction process of seaweed plant growth hormone is characterized in that this technology comprises the following steps: that 1. remove impurity and exotic with fresh marine alga with fresh water flushing, and with cutter mill clean marine alga is shredded; 2. adding absolute ethyl alcohol to concentration of alcohol in the marine alga of having shredded is 65～75%, and with the beater making beating, gets filtrate through suction filtration; 3. after extracting 22～26 hours, make extract; 4. remove moisture, ethanol and pigment with the rotary evaporation method and get concentrate; 5. concentrate is carried out centrifugal treating, remove the salinity that separates out, make the crude extract of clarification; 6. add excessive Reinecke's salt to form Reinecke's acid quaternary ammonium compound precipitation in crude extract, precipitation temperature is controlled at-1 ℃～-3 ℃, promptly gets product of the present invention again after centrifugal.
2, the extraction process of seaweed plant growth hormone according to claim 1 is characterized in that above-mentioned step 2 makes that also available ethanol merges in the filtrate to filter residue flushing 1～2 time and with washing lotion in the process of filtrate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011351373A CN1145416C (en) | 2001-11-22 | 2001-11-22 | Extracting process for seaweed plant growth hormone |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011351373A CN1145416C (en) | 2001-11-22 | 2001-11-22 | Extracting process for seaweed plant growth hormone |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1351830A CN1351830A (en) | 2002-06-05 |
| CN1145416C true CN1145416C (en) | 2004-04-14 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB011351373A Expired - Fee Related CN1145416C (en) | 2001-11-22 | 2001-11-22 | Extracting process for seaweed plant growth hormone |
Country Status (1)
| Country | Link |
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| CN (1) | CN1145416C (en) |
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| CN105503451A (en) * | 2016-02-22 | 2016-04-20 | 刘彬 | Production method for achieving cooperative production of major element and microelement type liquid water soluble fertilizer through seaweed extracts |
| CN105801202A (en) * | 2016-02-22 | 2016-07-27 | 刘彬 | Method for co-producing macroelement and medium element water-soluble fertilizer by utilizing seaweed extract |
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| CN105801206A (en) * | 2016-02-26 | 2016-07-27 | 刘彬 | Method for co-producing humic acid containing water-soluble fertilizer by utilizing seaweed extract |
| CN105753533A (en) * | 2016-02-26 | 2016-07-13 | 刘彬 | Production method for coproducing organic and inorganic seaweed fertilizers from seaweed extracting solution |
| CN105801222A (en) * | 2016-02-26 | 2016-07-27 | 刘彬 | Production method for coproducing seaweed shotcrete compound fertilizer by utilizing seaweed extracting solution |
| CN105801193A (en) * | 2016-02-26 | 2016-07-27 | 刘彬 | Production method for co-producing seaweed bio-organic fertilizer by utilizing seaweed extract |
| CN105753534A (en) * | 2016-02-26 | 2016-07-13 | 刘彬 | Production method for coproducing special fertilizer for rice from seaweed extracting solution |
| CN105801223A (en) * | 2016-02-26 | 2016-07-27 | 刘彬 | Production method for co-producing special corn fertilizer by utilizing seaweed extracting solution |
| CN105777337A (en) * | 2016-02-26 | 2016-07-20 | 刘彬 | Production method achieving co-production of micro-element liquid water-soluble fertilizer by means of seaweed extracting solution |
| CN105732202A (en) * | 2016-02-26 | 2016-07-06 | 刘彬 | Production method for utilizing seaweed extract for co-producing secondary element water-soluble fertilizer |
| CN105541498A (en) * | 2016-02-26 | 2016-05-04 | 刘彬 | Production method for co-producing humic-acid-containing liquid water-soluble fertilizer with seaweed extract |
| CN105601396A (en) * | 2016-02-26 | 2016-05-25 | 刘彬 | Production method for co-production of amino acid-containing liquid water-soluble fertilizer by utilizing seaweed extracts |
-
2001
- 2001-11-22 CN CNB011351373A patent/CN1145416C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1351830A (en) | 2002-06-05 |
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