CN114540445A - Preparation method of amino acid polypeptide - Google Patents
Preparation method of amino acid polypeptide Download PDFInfo
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- CN114540445A CN114540445A CN202210213982.7A CN202210213982A CN114540445A CN 114540445 A CN114540445 A CN 114540445A CN 202210213982 A CN202210213982 A CN 202210213982A CN 114540445 A CN114540445 A CN 114540445A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
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- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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Abstract
The invention discloses a preparation method of amino acid polypeptide, which comprises the following steps of S1, cutting and stirring livestock and poultry residues died of diseases to obtain meat paste I; s2, heating and preserving heat of the meat paste I for in-situ enzymatic hydrolysis to obtain meat paste II; s3, adding an aerobic microbial agent and an oxygen increasing agent into the meat paste II, and performing staggered hydrolysis to obtain hydrolyzed mash; s4, performing innocent treatment on the hydrolyzed mash to obtain a treatment solution; s5, filtering the treatment solution to obtain oil-water mixed solution and residues; and S6, standing and layering the oil-water mixed solution to obtain the grease and the amino acid polypeptide. Compared with the prior art, the method has the advantages of simple process, low requirement on equipment, no need of changing the existing production equipment and process, high yield of the prepared amino acid polypeptide, contribution to absorption and utilization of crops, no secondary pollution in the whole process, realization of harmless treatment of livestock and poultry died of diseases, and great environmental value and economic value.
Description
Technical Field
The invention belongs to the technical field of organic fertilizers, relates to a preparation method of amino acid polypeptide, and particularly relates to a preparation method of amino acid polypeptide by taking livestock and poultry died of diseases as raw materials.
Background
The livestock and poultry residues died of diseases are composed of muscles, internal organs, fur, bones and the like, and mainly comprise protein and fat. The harmless treatment process of the livestock and poultry died of diseases is generally to stir and cut the collected livestock and poultry died of diseases, then carry out high temperature treatment at 140 ℃ for 30min to obtain meat paste, evaporate and remove water to obtain meat powder which contains protein and fat and is used as a protein raw material of a fertilizer. After the protein raw material is subjected to evaporation treatment at 140 ℃ for a long time, the protein is highly denatured, is not beneficial to absorption and utilization of crops, and has poor using effect.
CN112321334A discloses a liquid amino acid fertilizer produced by using animal protein and a preparation method thereof, comprising the following steps: 1) mincing animals and/or animal leftovers to be treated to obtain minced materials; 2) heating and stirring animal and/or animal leftovers and alkali liquor at 50-90 deg.C to make pH above 13, hydrolyzing, standing for layering, and taking out the hydrolysate for use; 3) adjusting the pH of the hydrolysate to 8-9, adjusting the temperature to 60-90 ℃, and preserving the heat for 12-72 hours to obtain the amino acid fertilizer. The liquid amino acid fertilizer obtained by the invention can directly provide crop nutrition and is beneficial to crop absorption, but a large amount of alkali liquor is required to be added in the hydrolysis process, the requirement on corrosion resistance of equipment is high, the yield of the amino acid fertilizer is low, and the liquid amino acid fertilizer is not suitable for industrial production.
Therefore, it is highly desirable to develop a method for preparing amino acid polypeptides to overcome the deficiencies of the prior art.
Disclosure of Invention
In order to solve the technical problems, the invention provides a preparation method of amino acid polypeptide, which improves the yield of the amino acid polypeptide, and the prepared amino acid polypeptide has high plant utilization rate and is more beneficial to the absorption and utilization of crops.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for preparing an amino acid polypeptide, comprising the steps of:
s1, cutting and stirring the livestock and poultry residues died of diseases to obtain meat paste I;
s2, heating the meat paste I obtained in the step S1, preserving heat and hydrolyzing to obtain meat paste II;
s3, adding an aerobic microbial agent and an oxygen increasing agent into the meat paste II obtained in the step S2, and performing staggered hydrolysis to obtain hydrolyzed mash;
s4, performing high-temperature harmless treatment on the hydrolyzed mash obtained in the step S3 to obtain a treatment solution;
s5, filtering the treatment liquid obtained in the step S4 to obtain an oil-water mixed liquid and residues;
and S6, standing and layering the oil-water mixed liquid obtained in the step S5 to obtain grease and the amino acid polypeptide.
Preferably, the heating in step S2 is to be carried out to 48-52 ℃, and the time of heat preservation is 2.5-3.5 h.
Preferably, the aerobic bacterial agent in the step S3 is composed of Bacillus licheniformis, common Thermoactinomyces and Geobacillus.
Further preferably, the mass ratio of the bacillus licheniformis to the common thermoactinomyces and the bacillus terreus is 1-3: 0.5-1.5: 1; most preferably 2: 1: 1.
preferably, the addition amount of the aerobic bacterial agent is 10 by weight of the meat paste I6-107One per gram.
Preferably, the oxygenation agent in step S3 is sodium percarbonate or calcium percarbonate.
Further preferably, the oxygenation agent is added in an amount of 0.5-1% by weight of the meat paste I.
Preferably, the staggered hydrolysis in step S3 is: maintaining at 48-52 deg.C for 6-8 hr, increasing the temperature to 58-62 deg.C for 2.5-3.5 hr, and continuously heating to 68-72 deg.C for 2.5-3.5 hr.
Preferably, the high-temperature harmless treatment in the step S4 is: maintained at 140 ℃ for 30 min.
Preferably, the filtering in step S5 is: sieving with 300 mesh sieve at 95-100 deg.C.
Preferably, the temperature of the standing and layering in the step S6 is 95-98 ℃.
Preferably, the amino acid polypeptide may be any one of an amino acid polypeptide aqueous solution, an amino acid polypeptide concentrate, and an amino acid polypeptide powder.
The invention also provides application of the amino acid polypeptide prepared by the preparation method in amino acid water-soluble fertilizers, organic fertilizers and bio-organic fertilizers.
The invention also provides a fertilizer, which comprises the amino acid polypeptide prepared by the preparation method.
The invention has the beneficial effects that:
(1) according to the in-situ enzymatic hydrolysis, the meat paste is hydrolyzed by using an enzyme system existing in the livestock and poultry residues, the structure of protein is broken through from the inside of the meat paste to generate amino acid polypeptide, the generated amino acid polypeptide provides nutrition for the rapid growth of an aerobic microbial inoculum in the next step, and the design of long-time degradation (reproduction and hydrolysis), step-by-step temperature rise, step-by-step protein denaturation and osmotic hydrolysis is adopted by adding the aerobic microbial inoculum from the outside.
(2) The three substances separated by the preparation method disclosed by the invention have recycling value, the separated grease can be used as a raw material of biodiesel, residues can be used as a calcium-phosphorus raw material, amino acid polypeptide can be used as an organic nitrogen raw material, secondary pollution is not produced in the whole process, harmless treatment of livestock and poultry died of diseases is realized, and the preparation method has great environmental value and economic value.
(3) Compared with the prior art, the method has the advantages of simple process, low requirement on equipment, no need of changing production equipment and process, high yield of the prepared amino acid polypeptide, contribution to absorption and utilization of crops, and suitability for popularization and application.
Detailed Description
The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be easily understood by those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention.
Before the present embodiments are further described, it is to be understood that the scope of the invention is not limited to the particular embodiments described below; it is also to be understood that the terminology used in the examples is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention.
When numerical ranges are given in the examples, it is understood that both endpoints of each of the numerical ranges and any value therebetween can be selected unless the invention otherwise indicated. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In the embodiment, the bacillus licheniformis is purchased from China Industrial microorganism culture Collection management center, the product number is CICC 10092, the common high-temperature actinomycetes is purchased from China Industrial microorganism culture Collection management center, the product number is CICC 24226, and the adopted bacillus licheniformis is purchased from China agricultural microorganism culture Collection management center, and the product number is ACCC 02530.
A method for preparing an amino acid polypeptide, comprising the steps of:
s1, cutting and stirring: cutting and stirring livestock and poultry residues died of diseases to obtain meat paste I;
s2, in situ enzymatic hydrolysis: pumping the meat paste I obtained in the step S1 into a melting tank, heating to 48-52 ℃, maintaining for 2.5-3.5h, stirring for 1 time every 30min, stirring for 1-2min every time, and hydrolyzing the meat paste I by utilizing enzyme systems in livestock and poultry bodies to obtain meat paste II;
s3, alternate hydrolysis of aerobic bacteria: adding an aerobic microbial agent and an oxygen increasing agent into the meat paste II obtained in the step S2, culturing for 6-8h at 48-52 ℃, stirring for 1 time every 30 mm, stirring for 10min every time, enabling 3 bacteria to rapidly grow and propagate, generating a large amount of protease under the induction of protein, raising the temperature to 58-62 ℃, maintaining for 2.5-3.5h, stirring once every 30min, and stirring for 1-2min every time; then, the temperature is continuously increased to 68-72 ℃, the temperature is maintained for 2.5-3.5 hours, the stirring is carried out once every 30 minutes, the stirring is carried out for 1-2 minutes every time, in the process, aerobic bacteria agent is added from the outside, and the design of long-time degradation (reproduction and hydrolysis), step-by-step temperature rise, step-by-step protein denaturation and osmotic hydrolysis is adopted, so that the hydrolysis efficiency is improved by innovatively carrying out staggered hydrolysis with an enzyme system in the meat paste, cooperating with division and carrying out the combined action of the inside and the outside, and a product containing grease, amino acid polypeptide solution and residues, namely hydrolyzed mash, is obtained;
the aerobic microbial inoculum is prepared from the following components in a mass ratio of 1-3: 0.5-1.5: 1, bacillus licheniformis, common high-temperature actinomycetes and geobacillus;
the adding amount of the aerobic microbial inoculum is 10 in terms of the weight of the meat paste I6-107Per gram; the bacillus licheniformis and the bacillus terreus are activated in a liquid fermentation mode, the common high-temperature actinomycetes are activated in a solid fermentation culture mode, and the common high-temperature actinomycetes are added according to the number of spores;
the oxygenation agent is sodium percarbonate or calcium percarbonate, and the addition amount of the oxygenation agent is 0.5-1% of the weight of the meat paste I;
s4, innocent treatment: raising the temperature of the hydrolyzed mash obtained in the chemical preparation tank to 140 ℃, maintaining for 30min, and performing high-temperature harmless treatment to obtain a treatment solution;
s5, high-temperature filtration: filtering the treatment liquid obtained in the step S4 through a 300-mesh stainless steel filter screen at 95-100 ℃, wherein the filtered liquid is called oil-water mixed liquid, the filtered residue mainly comprises broken bones, and the residue is dried and crushed to be used as a raw material of calcium and phosphorus elements;
s6, oil-water separation: putting the oil-water mixed liquid obtained in the step S5 into a liquid storage tank, keeping the temperature at 95-98 ℃, standing for layering, removing oil in an overflow mode to obtain grease, naturally cooling, storing in a refrigeration house, and selling as a raw material of biodiesel, wherein the grease is not subjected to acid-base hydrolysis treatment, so that the heat value of an oil product is high; the liquid after oil removal is the amino acid polypeptide.
Wherein the solid content of the amino acid polypeptide is 10-17%.
Preferably, the amino acid polypeptide can be directly subjected to sterile filling to form an amino acid polypeptide solution; or pumping into a chemical tank, concentrating at 110 deg.C, and directly aseptic packaging to obtain amino acid polypeptide concentrate; the amino acid polypeptide concentrated solution can be spray-dried to prepare amino acid polypeptide powder with the water content of less than 13%.
The invention also provides application of the amino acid polypeptide prepared by the preparation method in amino acid water-soluble fertilizers, organic fertilizers and bio-organic fertilizers.
The invention also provides a fertilizer, which comprises the amino acid polypeptide prepared by the preparation method.
Example 1
A method for preparing an amino acid polypeptide, comprising the steps of:
s1, cutting and stirring: cutting and stirring livestock and poultry residues died of diseases to obtain meat paste I;
s2, in situ enzymatic hydrolysis: pumping the meat paste I obtained in the step S1 into a melting tank, heating to 50 ℃, maintaining for 3h, stirring for 1 time every 30min, stirring for 1-2min every time, and hydrolyzing the meat paste I by using an enzyme system in livestock and poultry to obtain meat paste II;
s3, alternate hydrolysis of aerobic bacteria: adding an aerobic microbial agent and an oxygen increasing agent into the meat paste II obtained in the step S2, culturing for 6h at 50 ℃, stirring for 1 time every 30 mm, stirring for 10min every time, enabling 3 bacteria to rapidly grow and propagate, raising the temperature to 60 ℃, maintaining for 3h, stirring once every 30min, and stirring for 1-2min every time; then continuously raising the temperature to 70 ℃, maintaining for 3h, stirring once every 30min, and stirring for 1-2min each time to obtain hydrolyzed mash;
the aerobic microbial inoculum is prepared from the following components in percentage by mass: 1: 1, bacillus licheniformis, common high-temperature actinomycetes and geobacillus;
the adding amount of the aerobic microbial inoculum is 10 in terms of the weight of the meat paste I6Per gram; the bacillus licheniformis and the bacillus terreus are activated in a liquid fermentation mode, the common high-temperature actinomycetes are activated in a solid fermentation culture mode, and the common high-temperature actinomycetes are added according to the number of spores;
the oxygenation agent is calcium percarbonate, and the addition amount of the oxygenation agent is 0.65 percent of the weight of the meat paste I;
s4, innocent treatment: raising the temperature of the hydrolyzed mash obtained in the chemical preparation tank to 140 ℃, maintaining for 30min, and performing high-temperature harmless treatment to obtain a treatment solution;
s5, high-temperature filtration: filtering the treatment liquid obtained in the step S4 through a 300-mesh stainless steel filter screen at 95-100 ℃, wherein the filtered liquid is called oil-water mixed liquid, the filtered residue mainly comprises broken bones, and the residue is dried and crushed to be used as a raw material of calcium and phosphorus elements;
s6, oil-water separation: putting the oil-water mixed liquid obtained in the step S5 into a liquid storage tank, keeping the temperature at 95-98 ℃, standing for layering, removing oil in an overflow mode to obtain grease, naturally cooling, storing in a refrigeration house, and selling as a raw material of biodiesel, wherein the grease is not subjected to acid-base hydrolysis treatment, so that the heat value of an oil product is high; the liquid after oil removal is the amino acid polypeptide.
Example 2
The present embodiment differs from embodiment 1 in that:
s2, in situ enzymatic hydrolysis: pumping the meat paste I obtained in the step S1 into a melting tank, heating to 52 ℃, maintaining for 2.5h, stirring for 1 time every 30min, stirring for 1-2min every time, and hydrolyzing the meat paste I by using enzyme systems in livestock and poultry bodies to obtain meat paste II;
s3, carrying out staggered hydrolysis on aerobic bacteria: adding an aerobic microbial agent and an oxygen increasing agent into the meat paste II obtained in the step S2, culturing for 7h at 52 ℃, stirring for 1 time every 30 mm, stirring for 10min every time, enabling 3 bacteria to rapidly grow and propagate, raising the temperature to 62 ℃, maintaining for 2.5h, stirring once every 30min, and stirring for 1-2min every time; then continuously raising the temperature to 72 ℃, maintaining for 2.5h, stirring once every 30min, and stirring for 1-2min every time to obtain hydrolyzed mash;
the aerobic microbial inoculum is prepared from the following components in percentage by mass of 3: 0.5: 1, bacillus licheniformis, common high-temperature actinomycetes and geobacillus;
the oxygenation agent is calcium percarbonate, and the addition amount of the oxygenation agent is 1% of the weight of the meat paste I.
Example 3
The present embodiment differs from embodiment 1 in that:
s2, in situ enzymatic hydrolysis: pumping the meat paste I obtained in the step S1 into a melting tank, heating to 48 ℃, maintaining for 3.5h, stirring for 1 time every 30min, stirring for 1-2min every time, and hydrolyzing the meat paste I by using enzyme systems in livestock and poultry bodies to obtain meat paste II;
s3, alternate hydrolysis of aerobic bacteria: adding an aerobic microbial agent and an oxygen increasing agent into the meat paste II obtained in the step S2, culturing for 8h at 48 ℃, stirring for 1 time every 30 mm, stirring for 10min every time, enabling 3 bacteria to rapidly grow and propagate, raising the temperature to 58 ℃, maintaining for 3.5h, stirring once every 30min, and stirring for 1-2min every time; then continuously raising the temperature to 68 ℃, maintaining for 3.5h, stirring once every 30min, and stirring for 1-2min every time to obtain hydrolyzed mash;
the aerobic microbial inoculum is prepared from the following components in a mass ratio of 1: 1.5: 1, bacillus licheniformis, common high-temperature actinomycetes and geobacillus;
the oxygenation agent is calcium percarbonate, and the addition amount of the oxygenation agent is 0.5 percent of the weight of the meat paste I.
Comparative example 1
This comparative example differs from example 1 in that: the aerobic microbial inoculum is prepared from the following components in percentage by mass: 1: 3, bacillus licheniformis, common high-temperature actinomyces and soil bacillus.
Comparative example 2
This comparative example differs from example 1 in that: the aerobic microbial inoculum is prepared from the following components in a mass ratio of 0.5: 4: 1 bacillus licheniformis, common high-temperature actinomycetes and geobacillus.
Comparative example 3
Amino acid fertilizer prepared according to CN 112321334A.
The yields of fats, residues and amino acid polypeptides obtained in the examples and comparative examples are shown in Table 1.
TABLE 1
As can be seen from Table 1, in the preparation method of the amino acid polypeptide, 1 ton of livestock and poultry died of diseases are put into the preparation method, the water content is 65 percent, 87 to 105kg of grease used as a raw material of biodiesel can be generated, 135-198kg of residues used as a raw material of calcium and phosphorus and 650kg of amino acid polypeptide solution are generated. Compared with the prior art, the three separated substances have recovery and use values, the whole process does not produce secondary pollution, and the method has great environmental value and economic value.
Meanwhile, the comparative examples 1 and 2 show that when the mass ratio of bacillus licheniformis, common high-temperature actinomyces and bacillus terrae in the aerobic microbial agent is changed, the solid content of the prepared amino acid polypeptide solution is less than 10 percent, so that the yield of the amino acid polypeptide powder is obviously reduced.
Example 4
The implementation place is as follows: the ink experimental field of Qingdao agricultural university, soil each item parameter: organic matter content 8.56g kg-1135 mg/kg of hydrolyzed nitrogen-168 mg/kg of available phosphorus-1Quick-acting potassium 325 mg/kg-1，pH6.30。
Experimental crops: strawberry
Subject: amino acid polypeptide solutions prepared in examples 1 to 3 and comparative examples 1 to 3, and clear water
The experimental method comprises the following steps: each group is provided with three repeated experiments, the mixture is compounded with trace element fertilizer (Mn + Zn + B is more than or equal to 20g/L), the same water and fertilizer conditions are adopted, after the strawberries are planted, amino acid polypeptide solution (diluted by 500 times by adding water) is sprayed for 3 times, the spraying is carried out for 1 time at intervals of 20-25d, the dosage of each time is 667m2150 ml.
The results of the experiment are shown in table 2.
TABLE 2
| Group of | Yield kg/mu |
| Example 1 | 1891 |
| Example 2 | 1768 |
| Example 3 | 1838 |
| Comparative example 1 | 1714 |
| Comparative example 2 | 1661 |
| Comparative example 3 | 1738 |
| Clean water | 1565 |
As shown in the table above, compared with the existing amino acid fertilizer, the amino acid polypeptide prepared by the invention can improve the strawberry acre yield, has higher plant absorption and utilization rate, and is more beneficial to the absorption and utilization of crops.
In conclusion, according to the in-situ enzymatic hydrolysis, the meat paste is hydrolyzed by using an enzyme system existing in the livestock and poultry residues, the structure of protein is broken through from the inside of the meat paste to generate amino acid polypeptide, the generated part of the amino acid polypeptide provides nutrition for the rapid growth of the next aerobic microbial inoculum, and the aerobic microbial inoculum is added from the outside, so that the designs of long-time degradation (reproduction and hydrolysis), step-by-step temperature rise, step-by-step protein denaturation and osmotic hydrolysis are adopted, the staggered hydrolysis with the enzyme system in the meat paste is innovative, the work is cooperatively divided, and the inside and the outside act together, so that the hydrolysis efficiency is improved, and the yield and the plant absorption rate of the amino acid polypeptide are greatly improved. Compared with the prior art, the method has the advantages of simple process, low requirement on equipment, no need of changing production equipment and process, high yield of the prepared amino acid polypeptide, contribution to absorption and utilization of crops, and suitability for popularization and application.
Claims (10)
1. A method for preparing an amino acid polypeptide, comprising the steps of:
s1, cutting and stirring the livestock and poultry residues died of diseases to obtain meat paste I;
s2, heating and preserving heat of the meat paste I obtained in the step S1 for in-situ enzymatic hydrolysis to obtain meat paste II;
s3, adding an aerobic microbial agent and an oxygen increasing agent into the meat paste II obtained in the step S2, and performing staggered hydrolysis to obtain hydrolyzed mash;
s4, performing innocent treatment on the hydrolyzed mash obtained in the step S3 to obtain a treatment solution;
s5, filtering the treatment liquid obtained in the step S4 to obtain an oil-water mixed liquid and residues;
and S6, standing and layering the oil-water mixed liquid obtained in the step S5 to obtain grease and the amino acid polypeptide.
2. The method according to claim 1, wherein the heating in step S2 is carried out to 48-52 ℃, and the time for the thermal hydrolysis is 2.5-3.5 h.
3. The method according to claim 1, wherein the aerobic bacterial agent in step S3 is composed of Bacillus licheniformis, Thermoactinomyces vulgaris and Geobacillus.
4. The preparation method according to claim 3, wherein the mass ratio of Bacillus licheniformis to Actinomyces vulgatus to Bacillus terreus is 1-3: 0.5-1.5: 1; preferably, the ratio of 2: 1: 1.
5. the preparation method of claim 1, wherein the amount of the aerobic bacterial agent added is 10% by weight of the meat paste I6-107One per gram.
6. The method according to claim 1, wherein the oxygen-increasing agent in step S3 is sodium percarbonate or calcium percarbonate.
7. The method according to claim 6, wherein the oxygen-increasing agent is added in an amount of 0.5-1% by weight of the meat paste I.
8. The method of claim 1, wherein the staggered hydrolysis in step S3 is: hydrolyzing at 48-52 deg.C for 6-8h, heating to 58-62 deg.C for 2.5-3.5h, and heating to 68-72 deg.C for 2.5-3.5 h.
9. The application of the amino acid polypeptide prepared by the preparation method according to any one of claims 1 to 8 in amino acid water-soluble fertilizers, organic fertilizers and bio-organic fertilizers.
10. A fertilizer comprising the amino acid polypeptide produced by the production method according to any one of claims 1 to 8.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2004021797A1 (en) * | 2002-07-29 | 2004-03-18 | Aminotech As | Method for production of peptides/amino acids produced by said method and use of the same |
| CN109627056A (en) * | 2019-01-21 | 2019-04-16 | 四川清和福生生物工程有限公司 | A kind of production method of paper mill sludge quick fermentation production biological organic fertilizer |
| CN110407623A (en) * | 2019-07-23 | 2019-11-05 | 武汉益锦祥生物环保有限公司 | A method of high-valued fertilizer is prepared based on dead livestock and poultry harmless treatment |
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- 2022-03-07 CN CN202210213982.7A patent/CN114540445B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004021797A1 (en) * | 2002-07-29 | 2004-03-18 | Aminotech As | Method for production of peptides/amino acids produced by said method and use of the same |
| CN109627056A (en) * | 2019-01-21 | 2019-04-16 | 四川清和福生生物工程有限公司 | A kind of production method of paper mill sludge quick fermentation production biological organic fertilizer |
| CN110407623A (en) * | 2019-07-23 | 2019-11-05 | 武汉益锦祥生物环保有限公司 | A method of high-valued fertilizer is prepared based on dead livestock and poultry harmless treatment |
Non-Patent Citations (1)
| Title |
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| 刘连成;陆正清;: "高温DXG复合菌剂对堆肥腐熟的影响", 河南农业科学 * |
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