CN114538625A - Self-maintenance bacteria and algae symbiotic aerobic granular sludge and rapid culture method and culture system thereof - Google Patents
Self-maintenance bacteria and algae symbiotic aerobic granular sludge and rapid culture method and culture system thereof Download PDFInfo
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Abstract
The invention relates to the technical field of sewage biological treatment, in particular to self-maintenance bacteria-algae symbiotic aerobic granular sludge and a rapid culture method and a culture system thereof. The rapid culture method comprises the following steps of: obtaining fungal spores of aspergillus niger and preparing spore suspension; culturing the spore suspension to form mycelium pellets; co-culturing the mycelium pellet and the algae liquid to obtain mycelium pellet wrapped algae coagulated nuclei; inoculating activated sludge in an illumination sequencing batch bioreactor, and then adding mycelium pellets to wrap algae coagulation nuclei; and operating the illumination sequencing batch bioreactor under the non-aeration condition to obtain completely granulated aerobic granular sludge symbiotic with bacteria and algae. The technical scheme solves the technical problems of long preparation period, high energy consumption, easy loss of biomass and poor pollutant removal effect of aerobic granular sludge. The aerobic granular sludge symbiotic with bacteria and algae has the advantages of large average particle size, compact granular structure, good settling property, excellent pollutant removal effect and the like, and has good application and popularization prospects.
Description
Technical Field
The invention relates to the technical field of sewage biological treatment, in particular to self-maintenance bacteria-algae symbiotic aerobic granular sludge and a rapid culture method and a culture system thereof.
Background
Aerobic Granular Sludge (AGS) is Granular activated Sludge formed by microbial self-aggregation. It has the characteristics of difficult sludge bulking, strong shock resistance, capability of bearing high organic load, integration of microorganisms with different properties (aerobic, facultative and anaerobic microorganisms), and the like. However, the cultivation period of the common aerobic granular sludge is long and the cultivation energy consumption is high, and how to reduce the preparation difficulty of the aerobic granular sludge, reduce the preparation energy consumption and improve the pollutant removal capability of the aerobic granular sludge is a problem that researchers and researchers in the field have long been dedicated to solve.
Chinese patent CN106006950B reports a method for promoting aerobic sludge granulation by mycelium pellets, which mainly comprises the following steps: preparing mycelium pellets; adding mycelium pellets into the SBR reactor; starting a reactor and culturing aerobic granular sludge; in the starting and running process of the reactor, properties such as the particle size, appearance shape, sedimentation performance, density and the like of aerobic granular sludge are regularly observed, effluent quality is regularly measured, and the sludge granulation degree and the long-term running stability degree of the granular sludge are analyzed and judged. The scheme can shorten the culture period of sludge granulation to a certain extent and enhance the domestic sewage treatment efficiency of the aerobic granular sludge. However, only mycelium pellets are used for promoting granulation of aerobic sludge, and the problems of long time consumption, high energy consumption, easy biomass loss, poor pollutant removal effect of the aerobic granular sludge obtained by production and the like in the complete granulation process still exist.
Disclosure of Invention
The invention aims to provide a method for rapidly culturing self-sustaining bacteria-algae symbiotic aerobic granular sludge, and aims to solve the technical problems that the preparation process of aerobic granular sludge in the prior art is long in period, high in energy consumption, easy in biomass loss and poor in pollutant removal effect.
In order to achieve the purpose, the invention adopts the following technical scheme:
a fast culture method of self-sustaining bacteria-algae symbiotic aerobic granular sludge comprises the following steps in sequence:
the method comprises the following steps: obtaining fungal spores of aspergillus niger and preparing spore suspension;
step two: culturing the spore suspension to form mycelium pellets;
step three: co-culturing the mycelium pellet and the chlorella to obtain the mycelium pellet wrapped algae coagulated nucleuses;
step four: inoculating activated sludge in an illumination sequencing batch bioreactor, and then adding mycelium pellets to wrap algae coagulation nuclei;
step five: and operating the illumination sequencing batch bioreactor to obtain completely granulated aerobic granular sludge symbiotic with bacteria and algae.
The scheme also provides the bacteria-algae symbiotic aerobic granular sludge prepared by the quick culture method of the self-maintenance bacteria-algae symbiotic aerobic granular sludge.
The principle and the advantages of the scheme are as follows:
the technical scheme provides a method for rapidly culturing self-maintenance bacteria-algae symbiotic aerobic granular sludge by utilizing mycelium pellets to wrap algae coagulation nuclei, the scheme can simultaneously realize rapid granulation of the sludge and stable operation of a self-maintenance bacteria-algae symbiotic aerobic granular sludge system, and the granular sludge cultured by the method has the advantages and characteristics of large particle size, good settling property, compact structure, strong pollutant removal capability and the like.
The technical scheme prepares self-sustaining bacteria and algae symbiotic aerobic Granular Sludge (ABGS), which is an environmentally sustainable, economical and feasible urban sewage treatment product. Compared with the conventional Aerobic Granular Sludge (AGS), the ABGS has remarkable advantages and characteristics, including compact structure, high biological retention, good sedimentation performance, good pollutant removal effect, large biomass resource recovery potential and the like. In the initial stage of ABGS formation, micro aggregates formed by the mutual combination of algae cells can be used as the core for the adhesion of microorganisms, the unique structure is favorable for maintaining the stability of the ABGS system, and the sludge granulation can be accelerated by utilizing the initial coagulation nucleus of algae. However, it is difficult to form an initial core by self-aggregation due to characteristics of small size of algal cells, poor settling property, slow growth rate, strong electrostatic repulsive force, and the like. Therefore, how to realize the rapid flocculation and nucleation of algae cells is a key problem. Through extensive research and screening, the inventor finds that the technical problem can be ideally overcome by introducing the mycelium pellet into an ABGS culture system.
The mycelium pellets are quasi-spherical particle aggregates which are formed by continuously growing and winding mycelium germinated from filamentous fungus spores under the condition of proper directional water flow shearing force and have compact surfaces and loose interiors. The mycelium pellet has many net-shaped gaps, large specific surface area and good settling property. A layer of viscous Extracellular Polymeric Substance (EPS) film is attached to the surface of the mycelium, when the microalgae is cultured with the mycelium pellet, algae cells can be attached to the mycelium of the fungus through the trapping or adhesion action of the mycelium pellet and are rapidly fixed in the mycelium pellet to form an algae-fungal mycelium pellet complex. A related research for accelerating the rapid formation of the self-sustaining bacteria-algae symbiotic aerobic granular sludge by using the fungus-algae mycelial sphere complex as a coagulation nucleus (namely, the mycelial sphere wraps the coagulation nucleus of algae) is not reported.
In conclusion, the beneficial effects of the technical scheme are as follows:
(1) the scheme is to implement biological enhancement based on the natural formation rule of ABGS, and the mycelium pellet is wrapped by algae and coagulated nuclei and is added into a non-aerated illumination sequencing batch bioreactor, so that the particles with the particle size of more than 0.3mm and SVI can be formed in 12 days30/SVI5The ABGS with the ratio of more than 0.8 realizes the rapid granulation of the bacteria-algae symbiotic sludge.
(2) The ABGS cultured by the scheme has larger average particle size (2-5mm), compact particle structure (sludge density is 1.0-1.2g/mL) and good sedimentation performance (SVI)3030-60 mL/g). In addition, the pollutant removal effect is excellent, wherein the removal rates of COD, total nitrogen and total phosphorus are respectively more than 95%, 80% and 75%.
(3) Organic matters generated by algae in the sludge can slow down collapse of an anaerobic inner core caused by endogenous respiration of microorganisms in a hungry state for a long time, and the stability of the system is improved.
Further, in step one, Aspergillus niger was cultured in potato dextrose agar plates at 20-40 deg.CObtaining fungal spores in 1-7 days; preparing spore suspension from fungal spore, and adjusting the concentration of spore suspension to 102-108CFU/mL. The concentration of the spore suspension has a remarkable influence on the effective formation of the algae coagulation nuclei wrapped by the mycelial pellets, and both too low and too high can influence the granulation process of the sludge and the performance of the aerobic granular sludge.
Further, in the second step, the spore suspension is inoculated into a Chaudhur culture medium, and cultured for 1-5 days under the conditions that the initial pH is 4-8, the temperature is 20-40 ℃, and the rotating speed is 100-. When the mycelium pellets are cultured, conditions such as initial pH, rotating speed and the like have obvious influence on the formation and activity of the mycelium pellets, proper parameter conditions are selected, and the particle size of the mycelium pellets is maintained within a certain range, so that the subsequently obtained mycelium pellets wrapped with algae condensation nuclei have ideal nucleation performance, sludge granulation can be realized more quickly, and the pollutant treatment effect of granular sludge is improved.
Further, in the third step, adding the mycelium pellet with the wet weight of 1-15g into 30-100mL of chlorella-containing algae liquid, and co-culturing for 12-24h at the temperature of 20-40 ℃ and the speed of 100-180rpm to obtain the mycelium pellet wrapped algae coagulated nucleus; the density of chlorella in the chlorella solution is 105-108One per mL.
The inventor's earlier research results show that the ABGS system can realize complete granulation only in 20-60 days, and in the granulation process, the problems of high energy consumption of mechanical aeration, easy loss of biomass, poor pollutant removal effect and the like still exist, so that the large-scale popularization and application of the technology are limited. Through a great deal of research on the second step and the third step, the inventor finds that the culture of the mycelial pellets and the co-culture mode of the mycelial pellets and algae cells have very remarkable influence on the granulation period of the aerobic granular sludge symbiotic with the mycelial algae and the pollutant treatment effect. By adopting the technical scheme, the aerobic granular sludge with symbiosis of bacteria and algae, which has short granulation period and strong pollutant removal capability, can be prepared.
Further, in the fourth step, the effective volume of the illumination sequencing batch bioreactor is 0.5-5L, and the illumination intensity is 2000-; inoculating activated sludge into an illumination sequencing batch bioreactor, and controlling the suspended solid concentration of the initial mixed liquor to be 2-10 g/L; the dry weight ratio of the mycelium pellet wrapped algae coagulated nucleus to the activated sludge is 1-50%.
Further, in the fifth step, the operation mode of the illumination sequencing batch bioreactor is as follows: circularly operating according to the sequence of water inlet, oscillation, precipitation and drainage; the water inlet time is 1-5min, the oscillation time is 705-717min, the sedimentation time is 1-5min, and the water drainage time is 1-5 min; in the operation process, the sludge retention time in the illumination sequencing batch bioreactor is controlled to be 15-30 days, and the water drainage ratio of the illumination sequencing batch bioreactor is controlled to be 30-70%.
By adopting the technical parameters, the sludge granulation and pollutant removal effects can be ensured.
Furthermore, a water inlet substrate used by the illumination sequencing batch bioreactor is simulated domestic sewage; in the simulated domestic sewage, the COD concentration is 200-700mg/L, NH4 +PO with-N concentration of 10-100mg/L4 3--the concentration of P is 5-20 mg/L; the rotation speed of the illumination sequencing batch bioreactor is 100-180 rpm.
Further, in the fifth step, the average grain diameter of the completely granulated aerobic granular sludge symbiotic with bacteria and algae is more than 0.3 mm; SVI30/SVI5Is greater than 0.8. Sludge volume index SVI30/SVI5When the ratio of the aerobic granular sludge to the aerobic granular sludge is more than 0.8 and the grain diameter of the granules is more than 0.3mm, the aerobic granular sludge produced by symbiosis of bacteria and algae is granulated, and the aerobic granular sludge has a relatively ideal sewage treatment effect.
Further, the scheme also provides a culture system used in the method for rapidly culturing the self-sustaining bacteria-algae symbiotic aerobic granular sludge, which is characterized by comprising an illumination sequencing batch bioreactor and water inlet and outlet control equipment; under the working state, the illumination sequencing batch bioreactor is fixed in a shaking table; the water inlet and outlet control equipment comprises a water inlet tank, a water outlet tank, a water inlet peristaltic pump and an automatic controller; the water inlet tank is used for supplying water to the illumination sequencing batch bioreactor, and the water outlet tank is used for receiving water flowing out of the illumination sequencing batch bioreactor; the quantity of the water inlet peristaltic pumps is two, one water inlet peristaltic pump is used for controlling the water inlet tank to supply water to the illumination sequencing batch bioreactor, and the other water inlet peristaltic pump is used for controlling the illumination sequencing batch bioreactor to discharge water to the water outlet tank; the automatic controller is used for controlling the operation of the two water inlet peristaltic pumps.
The bacteria-algae symbiotic aerobic granular sludge is cultured and prepared by the illumination sequencing batch bioreactor, and the bacteria-algae symbiotic aerobic granular sludge with ideal particle size, shape and sewage treatment effect can be obtained in a short period. The illumination sequencing batch bioreactor and the culture system have the advantages of simple structure, simple and convenient operation, low cost and excellent effect, can achieve the purposes of energy conservation and consumption reduction, are easy for industrial expansion, and are suitable for large-scale production of the aerobic granular sludge symbiotic with bacteria and algae.
Drawings
FIG. 1 is a schematic view of a bacteria-algae symbiotic aerobic granular sludge culture system according to embodiment 1 of the present invention.
FIG. 2 is a micrograph of aerobic granular sludge symbiotic with bacteria and algae at day 32 according to example 1 of the present invention.
FIG. 3 is a micrograph of aerobic granular sludge symbiotic with bacteria and algae at day 32 of comparative example 1 of the present invention.
Detailed Description
The present invention will be described in further detail with reference to embodiments, but the embodiments of the present invention are not limited thereto. Unless otherwise indicated, the following techniques are conventional and well known to those skilled in the art: the materials, reagents and the like used are all commercially available.
The attached figure 1 is marked as: a water inlet tank 1, a water inlet peristaltic pump 2, an automatic controller 3, a water outlet tank 4, a shaking table 5, activated sludge 6, a mycelium pellet wrapped algae coagulation nucleus 7 and an illumination sequencing batch bioreactor 8.
Example 1:
the method comprises the following steps: preparation of spore suspension
(1) Aspergillus niger (AS 3.3928) was cultured in Potato Dextrose Agar (PDA) plates at 37 ℃ for 7 days until fungal spores were produced.
(2) Fungal spores were gently scraped off the PDA plate and placed in a 250mL glass flask, and 100mL of sterile water was added to prepare a spore suspension.
Step two: preparation of mycelium pellets
Diluting spore suspension to 106CFU/mL, inoculating 1mL spore suspension into 100mL sterile liquid Chachien (Czapek) medium, culturing at the conditions of initial pH of the medium solution of 6, temperature of 30 ℃ and rotation speed of 150rpm to obtain mycelium pellets with a particle size of about 1mm (in this example, the culture time is about 3 days, and generally, the culture time is 1-5 days, and the particle size of the mycelium pellets can reach 0.5-5 mm). Filtering with sterile gauze to obtain mycelium pellet, washing with sterile water for 3-5 times, soaking in sterile physiological saline, and refrigerating at 4-6 deg.C.
Step three: preparation of mycelium pellet wrapped with algae coagulated nucleus
The method for preparing the mycelium pellet wrapped with the coagulated nucleus is an adsorption method, and comprises the steps of mixing 12g (wet weight) of the mycelium pellet obtained in the step two with 50mL of algae liquid (Chlorella, Chlorella, FACHB-31, the algae density is 8.5 multiplied by 10)6one/mL) is put into a sterile triangular flask and is shake-cultured for 24h under the conditions of 30 ℃ and 150rpm (at the moment, the flocculation efficiency of algae cells is close to 100 percent), and the mycelium pellet is wrapped with the algae coagulation nucleus. Filtering with sterile gauze to obtain mycelium pellet wrapped with algae coagulated nucleus, cleaning with sterile water for 3-5 times, soaking in sterile physiological saline, and refrigerating at 4-6 deg.C.
Step four: adding of mycelium pellets wrapped with algae coagulation nuclei
(1) Placing a glass triangular flask (PSBR Reactor) with the effective volume of 0.5L into an illumination shaking table, setting the illumination intensity to be 3000Lux, and completing the construction of a Photo-Sequencing Batch bioreactor (PSBR).
(2) 150mL of activated sludge from a secondary sedimentation tank of a sewage treatment plant was inoculated into a light sequencing batch bioreactor (PSBR reactor), and 250mL of simulated domestic sewage was added to make the initial MLSS (Mixed Liquor Suspended Solids concentration) of the reactor 3.7 g/L.
(3) The mycelium pellet wrapped coagulation nuclei are added into an illumination sequencing batch bioreactor (PSBR) according to the adding ratio of 2.5% (namely the dry weight ratio of the mycelium pellet wrapped algae coagulation nuclei to the activated sludge), and the operation is started. The dry weight of the mycelial pellets wrapped with the algae coagulated nuclei and the activated sludge refers to the weight of the two substances after the moisture of the two substances is completely dried. Sampling and detecting the dry weight of the mycelium pellet wrapped with the algae coagulated nucleus and the activated sludge, measuring for many times, taking an average value, and calculating to obtain the corresponding conditions of the dry weight and the wet weight of the mycelium pellet wrapped with the algae coagulated nucleus and the activated sludge. And then calculating the feeding amount of fresh substances required by the mycelium pellet wrapped algae coagulation nucleus under the set feeding ratio (for example, 2.5%), and then feeding the calculated amount of the mycelium pellet wrapped algae coagulation nucleus into the activated sludge.
Step five: operation of PSBR reactor
(1) The operation mode of the illumination sequencing batch bioreactor is carried out according to the sequence of water inlet, oscillation, precipitation and water discharge, each period is 12 hours, wherein: the water inlet Time is 1min, the oscillation Time is 712-;
(2) a water inlet substrate: artificially prepared simulated domestic sewage, wherein: COD is 600mg/L, NH4+-N is 60mg/L, PO4 3-P is 10mg/L, and MgSO with a final concentration of 25mg/L is added4·7H2O, FeSO with final concentration of 30mg/L4·7H2O, CaCl with final concentration of 30mg/L2And adding the trace element solution according to the proportion of 1.0 mL/L. The trace elements comprise the following components in percentage by weight: FeCl3·6H2O 0.5g/L、H3BO3 0.15g/L、CuSO4·5H2O 0.05g/L、MnCl2·4H2O 0.15g/L、Na2MoO4·2H2O 0.1g/L、ZnSO4·7H2O 0.15g/L、CoCl2·6H2O 0.15g/L、EDTA 0.05g/L。
(3) The whole illumination sequencing batch bioreactor is operated without aeration equipment and is operated at the rotating speed of 150 rpm.
In addition, a schematic diagram of a self-sustaining bacteria-algae symbiotic aerobic granular sludge culture system is shown in figure 1. The cultivation system comprises a PSBR reactor 8 (light sequencing batch bioreactor) and a water inlet and outlet control device. In operation, the PSBR reactor 8 is mounted in the cradle 5 by conventional means of the prior art and a strip of light is wound around the PSBR reactor 8. The water inlet and outlet control equipment comprises a water inlet tank 1, a water outlet tank 4, a water inlet peristaltic pump 2 and an automatic controller 3. The automatic controller 3 is a conventional device for controlling the water inlet peristaltic pump 2 in the prior art, and the two water inlet peristaltic pumps 2 respectively control water inlet from the water inlet tank 1 to the PSBR reactor 8 and water outlet from the PSBR reactor 8 to the water outlet tank 4. The water inflow of the technical scheme is artificially prepared simulated domestic sewage.
In this example, the average particle size of the sludge granules is greater than 0.3mm after 12 days of culture, SVI30/SVI5The ratio is 0.87 (sludge volume index SVI)30And SVI5Ratio of (d). The conditions for completely granulating the sludge are that the particle diameter is more than 0.3mm and SVI30/SVI5Is greater than 0.8. According to the above criteria, the sludge in this example achieved complete granulation of ABGS within 12 days. As shown in FIG. 2, the granular sludge at day 32 has clear contour (average particle diameter of 3.3mm), compact structure (relative wet density of sludge of 1.0253g/mL), high algae cell retention (chlorophyll alpha concentration of 3.8mg/L), and good sludge settling property (SVI)30/SVI5Is 0.99, SVI3053.2mL/g) and has a high efficiency of contaminant removal, as reflected in the data: COD, TN (Total Nitrogen) and PO4 3-The removal rates of-P (total phosphorus) were 98.6%, 80.8% and 80.0%, respectively. In this example, COD, suspended solids concentration (MLSS), Sludge Volume Index (SVI)5And SVI30) The technical indexes such as chlorophyll alpha concentration, sludge relative wet density, average particle size, total nitrogen, total phosphorus and the like are all determined by adopting a national standard analysis method.
Example 2 and example 3 are basically the same as the examples except for the partial parameter settings, which are detailed in table 1.
Table 1: parameter settings and product Performance test results of examples 1-3
Comparative example 1
The culture method of the self-maintenance bacteria-algae symbiotic aerobic granular sludge of the comparative example is basically the same as that of the example 1, except that mycelium pellets are not added to wrap algae coagulation nuclei, and only activated sludge and algae cells (50mL, 8.5X 10) which are equal to those in the example 1 are added6one/mL).
In this comparative example, complete granulation of the sludge was achieved after 28 days, and the granulation time was significantly longer than that required in example 1 (16 days longer). As shown in FIG. 3, the average particle size of comparative example 1 was smaller, the amount of granular sludge in a crushed state was larger, and the average particle size was 2.6mm, which was significantly smaller than that of the granules in example 1, at day 32. Comparative example 1 the sludge had a relative wet density of 1.0184g/mL, a chlorophyll alpha concentration of 1.5mg/L, and an SVI30/SVI5Is 0.81, SVI3065.8mL/g, low efficiency of removing pollutants, COD, TN and PO4 3-The removal rates of-P were 96.4%, 67.5% and 70.0%, respectively. The results show that the addition of mycelial pellets to wrap the algae coagulation nuclei can effectively accelerate the formation of self-maintenance bacteria-algae symbiotic aerobic granular sludge, and simultaneously improve the pollutant removal capacity, and the compact granular structure of the aerobic granular sludge is beneficial to maintaining the stability of the system.
Comparative example 2
The culture method of the self-maintenance bacteria-algae symbiotic aerobic granular sludge of the comparative example is basically the same as that of the example 1, and the difference is that no mycelium pellet is added to wrap algae coagulation nuclei, only mycelium pellets and the same amount of activated sludge are added, and algae cells (50mL, 8.5 multiplied by 10) are added6one/mL). The putting amount of the mycelium pellets is as follows: and (3) putting the mycelium pellets into the activated sludge according to the adding ratio (namely the dry weight ratio of the mycelium pellets to the activated sludge) of 2.5%, wherein the mycelium pellets are obtained by the preparation in the second step of the example 1.
In the present comparative example, complete granulation of the sludge was achieved after 20 days. At day 32, the chlorophyll alpha concentration was 2.8mg/L, SVI30/SVI50.88, COD, TN and PO4 3-The removal rates of-P were 97.8%, 79.2% and 75.0%, respectively.
Comparative examples 3 to 9 are substantially the same as example 1, except for the adjustment of the technical parameters of step two, as detailed in table 2.
Table 2: comparative examples 3 to 9
Comparative example 10-comparative example 15 are substantially the same as example 1 except for the adjustment of the technical parameters in step three, as detailed in table 3.
Table 3: comparative example 10 to comparative example 15
Analysis of experimental results of examples and comparative examples:
according to the experimental results in table 1, the self-sustaining bacteria-algae symbiotic aerobic granular sludge prepared by the method of wrapping the mycelium pellets with the algae coagulation nuclei has a relatively ideal pollutant removal effect and has the advantages of relatively large particle size, good settling property, compact structure, high pollutant removal capacity and the like. And overcomes the defects of small size, poor settling property, slow growth speed, strong electrostatic repulsion and the like of algae cells, quickly forms an initial core under the cooperation of the mycelium pellet, and shortens the time of sludge granulation.
In comparative example 1, only algal cells were used, the sludge granulation time was long, and the pollutant removing effect was limited. In comparative example 2, mycelial pellets and algal cells were not co-cultured, and there were also problems of long sludge granulation time and limited pollutant removal effect. In the embodiment, the algae cells and the mycelial pellets are combined in a pre-co-culture mode, so that the sludge granulation time is obviously reduced, and the pollutant treatment effect of aerobic sludge granules is obviously enhanced. The experiments prove that the combined use of the algae cells and the mycelium pellets has a synergistic effect, and has a remarkable improvement effect on reducing the preparation difficulty of the aerobic granular sludge and increasing the pollutant treatment effect.
In comparative examples 3-9, the technical parameters of step two were studied and explored. The spore suspension liquid with too high concentration, too high or too low initial pH, too high or too low mycelium pellet particle size and too high or too low rotating speed during mycelium pellet culture can obviously influence the final sludge complete granulation time and the pollutant removal effect and the sedimentation performance of aerobic granular sludge. In comparative examples 10 to 15, each technical parameter of step three was studied and explored. The volume of algae liquid (the total amount of algae cells) is too high or too low, the using amount of mycelium pellets is too high or too low, and the co-culture time is too long or too short, so that the final sludge complete granulation time and the pollutant removal effect and the sedimentation performance of aerobic granular sludge are obviously influenced.
The foregoing is merely an example of the present invention and common general knowledge in the art of designing and/or characterizing particular aspects and/or features is not described in any greater detail herein. It should be noted that, for those skilled in the art, without departing from the technical solution of the present invention, several variations and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (10)
1. A fast culture method of self-maintenance bacteria and algae symbiotic aerobic granular sludge is characterized by comprising the following steps of:
the method comprises the following steps: obtaining fungal spores of aspergillus niger and preparing spore suspension;
step two: culturing the spore suspension to form mycelium pellets;
step three: co-culturing the mycelium pellet and the chlorella to obtain the mycelium pellet wrapped algae coagulated nucleuses;
step four: inoculating activated sludge in an illumination sequencing batch bioreactor, and then adding mycelium pellets to wrap algae coagulation nuclei;
step five: and operating the illumination sequencing batch bioreactor to obtain completely granulated aerobic granular sludge symbiotic with bacteria and algae.
2. The fast culture method of the aerobic granular sludge symbiotic with self-sustaining bacteria and algae as claimed in claim 1, wherein in step one, Aspergillus niger is cultured in potato dextrose agar plate at 20-40 deg.C for 1-7 days to obtain fungal spore; preparing spore suspension from fungal spore, and adjusting the concentration of spore suspension to 102-108CFU/mL。
3. The fast culture method of self-sustaining bacteria-algae symbiotic aerobic granular sludge as claimed in claim 1, wherein in step two, the spore suspension is inoculated into Chaudhur's culture medium and cultured for 1-5 days under the conditions of initial pH of 4-8, temperature of 20-40 ℃, and rotation speed of 100-180rpm to obtain mycelium pellets with particle size of 0.5-5 mm.
4. The fast culture method of self-sustaining mycelial algae symbiotic aerobic granular sludge as claimed in claim 1, wherein in the third step, mycelial pellets with wet weight of 1-15g are added into 30-100mL of chlorella-containing algae liquid, and co-cultured for 12-24h at 20-40 ℃ and 100-180rpm to obtain mycelial pellet wrapped algae coagulated nuclei; the density of chlorella in the chlorella solution is 105-108one/mL.
5. The method for rapidly culturing the self-sustaining bacteria-algae symbiotic aerobic granular sludge as claimed in claim 1, wherein in the fourth step, the effective volume of the illumination sequencing batch bioreactor is 0.5-5L, and the illumination intensity is 2000-8000 Lux; inoculating activated sludge into an illumination sequencing batch bioreactor, and controlling the suspended solid concentration of the initial mixed liquor to be 2-10 g/L; the dry weight ratio of the mycelium pellet wrapped algae coagulated nucleus to the activated sludge is 1-50%.
6. The method for rapidly culturing the self-sustaining aerobic symbiotic bacteria-algae granular sludge as claimed in claim 1, wherein in the fifth step, the operation mode of the illumination sequencing batch bioreactor is as follows: circularly operating according to the sequence of water inlet, oscillation, precipitation and drainage; the water inlet time is 1-5min, the oscillation time is 705-717min, the sedimentation time is 1-5min, and the water drainage time is 1-5 min; in the operation process, the retention time of sludge in the illumination sequencing batch bioreactor is controlled to be 15-30 days, and the drainage ratio of the illumination sequencing batch bioreactor is controlled to be 30-70%.
7. The method for rapidly culturing the self-sustaining bacteria-algae symbiotic aerobic granular sludge according to claim 6, wherein the water inlet substrate used by the illumination sequencing batch bioreactor is simulated domestic sewage; in the simulated domestic sewage, the COD concentration is 200-700mg/L, NH4 +PO with-N concentration of 10-100mg/L4 3--the concentration of P is 5-20 mg/L; the rotation speed of the illumination sequencing batch bioreactor is 100-180 rpm.
8. The method for rapidly culturing the self-sustaining aerobic granular symbiotic bacteria-algae sludge as claimed in claim 7, wherein in step five, the average particle size of the fully granulated aerobic granular symbiotic bacteria-algae sludge is greater than 0.3mm, and SVI is adopted30/SVI5＞0.8。
9. The bacteria-algae symbiotic aerobic granular sludge prepared by the self-maintenance bacteria-algae symbiotic aerobic granular sludge rapid culture method according to any one of claims 1 to 8.
10. The culture system used in the fast culture method of the self-sustaining bacteria-algae symbiotic aerobic granular sludge according to any one of the claims 1 to 8, is characterized by comprising an illumination sequencing batch bioreactor and a water inlet and outlet control device; under the working state, the illumination sequencing batch bioreactor is fixed in a shaking table; the water inlet and outlet control equipment comprises a water inlet tank, a water outlet tank, a water inlet peristaltic pump and an automatic controller; the water inlet tank is used for supplying water to the illumination sequencing batch bioreactor, and the water outlet tank is used for receiving water flowing out of the illumination sequencing batch bioreactor; the quantity of the water inlet peristaltic pumps is two, one water inlet peristaltic pump is used for controlling the water inlet tank to supply water to the illumination sequencing batch bioreactor, and the other water inlet peristaltic pump is used for controlling the illumination sequencing batch bioreactor to discharge water to the water outlet tank; the automatic controller is used for controlling the operation of the two water inlet peristaltic pumps.
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