CN114524867B - 一种影响鳞翅目昆虫取食的植物内源基因及其蛋白 - Google Patents

一种影响鳞翅目昆虫取食的植物内源基因及其蛋白 Download PDF

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CN114524867B
CN114524867B CN202210170559.3A CN202210170559A CN114524867B CN 114524867 B CN114524867 B CN 114524867B CN 202210170559 A CN202210170559 A CN 202210170559A CN 114524867 B CN114524867 B CN 114524867B
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张悦
刘春琴
王庆雷
赖德强
宋素胜
寇俊凤
冯晓洁
刘福顺
吴娱
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Abstract

本发明提供了一种影响鳞翅目昆虫取食的植物内源基因及其蛋白。其中的植物内源其蛋白可以影响昆虫取食所述植物的量，所述蛋白的氨基酸序列如SEQ ID No.2所示。

Description

一种影响鳞翅目昆虫取食的植物内源基因及其蛋白
技术领域
本发明涉及一种影响鳞翅目昆虫取食的植物内源基因及其蛋白，特别是缺失或沉默该基因后的纯合子植物能够获得对鳞翅目昆虫的抗性。
背景技术
在农业生产中，害虫是影响作物产量和品质的重要原因之一，每年都会给农民造成巨大经济损失。鳞翅目包括蛾、蝶两类昆虫，属有翅亚纲、全变态类。该目为昆虫纲中仅次于鞘翅目的第2个大目，分布范围极广，以热带种类最为丰富。绝大多数种类的幼虫为害各类栽培植物，体形较大者常食尽叶片或钻蛀枝干。体形较小者往往卷叶、缀叶、结鞘、吐丝结网或钻入植物组织取食为害。成虫多以花蜜等作为补充营养，或口器退化不再取食，一般不造成直接危害。目前，针对作物害虫的防治主要依赖于化学农药，但化学农药具有刺激气味，对环境和人身健康都有严重威胁，所以从自然资源中挖掘天然杀虫材料越来越受到人们重视。在植物中寻找天然的抗虫基因或影响昆虫取食的基因，对于开发新型天然抗虫品系作物具有重要的理论和实践意义。
发明内容
本发明之一提供了一种植物内源蛋白在影响昆虫取食所述植物中的应用，所述蛋白的氨基酸序列如SEQ ID No.2所示。
在一个具体的实施方式中，通过抑制所述植物内源蛋白、失活所述植物内源蛋白、沉默所述植物内源蛋白，抑制所述植物内源蛋白的表达、沉默所述植物内源蛋白的表达以及降低所述植物内源蛋白的表达量中的一种使所述植物获得对昆虫的抗性。
在一个具体的实施方式中，所述昆虫为鳞翅目昆虫中的至少一种。
在一个具体的实施方式中，所述昆虫为灰翅夜蛾属昆虫中的至少一种。
在一个具体的实施方式中，所述昆虫为草地贪夜蛾和/或甜菜夜蛾。
在一个具体的实施方式中，所述植物为拟南芥。
本发明之二提供了编码如本发明之一中任意一项所述的应用中的植物内源蛋白的核酸在影响昆虫取食所述植物中的应用。
在一个具体的实施方式中，通过对所述核酸进行突变得到突变型植物获得对昆虫的抗性。
在一个具体的实施方式中，所述突变为插入突变、缺失突变、移码突变中的一种。
在一个具体的实施方式中，所述突变为插入突变或缺失突变。
在一个具体的实施方式中，所述突变为T-DNA插入突变。
在一个具体的实施方式中，所述突变型植物为突变型纯合子。
在一个具体的实施方式中，所述植物为拟南芥。
在一个具体的实施方式中，所述核酸的序列如SEQ ID No.1所示。
在一个具体的实施方式中，所述昆虫为鳞翅目昆虫中的至少一种。
在一个具体的实施方式中，所述昆虫为灰翅夜蛾属昆虫中的至少一种。
在一个具体的实施方式中，所述昆虫为草地贪夜蛾。
本发明的有益效果：
本发明首次发现在植物中存在的cznky2020001基因可以吸引鳞翅目昆虫幼虫，特别是草地贪夜蛾或甜菜夜蛾幼虫的取食，而将cznky2020001基因从植物中敲除或基因的部分序列缺失的纯合子则可以抑制昆虫幼虫的取食，进而提高植物对鳞翅目昆虫幼虫，特别是对草地贪夜蛾或甜菜夜蛾幼虫的抗虫性。因此，可以据此通过将植物中的cznky2020001基因敲除、沉默或缺失获得纯合子植株以达到抑制昆虫幼虫的取食，进而提高植物抗虫性的目的。
附图说明
图1为cznky2020001的基因结构。
图2显示了14天后取食cznky2020001-1、cznky2020001-2和Col-0植株的草地贪夜蛾幼虫的平均单只虫重。
图3显示了14天后取食cznky2020001-1、cznky2020001-2和Col-0植株的甜菜夜蛾幼虫的平均单只虫重。
具体实施方式
以下通过优选的实施案例的形式对本发明的上述内容作进一步的详细说明，但其不构成对本发明的限制。
如无特别说明，本发明的实施例中的试剂均可通过商业途径购买。
在ABRC(https://abrc.osu.edu/)官网订购拟南芥Col-0(野生型)、cznky2020001T-DNA插入型突变体cznky2020001-1(种子号：SALK_018601)和cznky2020001T-DNA插入型突变体cznky2020001-2(种子号：SALK_015611)。图1显示了cznky2020001的基因结构。如图1所示，在cznky2020001-1中，T-DNA插入在第8个外显子处；在cznky2020001-2中，T-DNA插入在第12个外显子处。其中，cznky2020001的核酸序列如SEQID No.1所示，其蛋白的氨基酸序列SEQ ID No.2所示。
将拟南芥突变体cznky2020001-1和cznky2020001-2种子分别点铺在MS培养基上，待拟南芥植株长至4-6片叶子时将其移栽到蛭石上生长(24℃；光照:黑暗＝16h:8h)，45天后各取24株分别提取突变体植株叶片的DNA。
1.cznky2020001-1突变型纯合子的筛选
以引物F1(SEQ ID No.3)、引物R1(SEQ ID No.4)，以及以突变体cznky2020001-1各植株的DNA为模板进行PCR扩增，用来鉴定突变体cznky2020001-1的各植株是否在cznky2020001基因上存在T-DNA插入；以引物F2(SEQ ID No.5)、引物R1(SEQ ID No.4)，以及以突变体cznky2020001-1各植株的DNA为模板进行PCR扩增，用来鉴定突变体cznky2020001-1的各植株是否存在cznky2020001基因。将F1/R1引物对扩增后的PCR扩增产物显示约600bp，且F2/R1引物对扩增后无PCR扩增产物的植株确定为cznky2020001-1纯合突变体植株，即突变型纯合子。
2.cznky2020001-2突变型纯合子的筛选
以引物F1(SEQ ID No.3)、引物R2(SEQ ID No.6)，以及以突变体cznky2020001-2各植株的DNA为模板进行PCR扩增，用来鉴定突变体cznky2020001-2各植株在cznky2020001基因上是否存在T-DNA插入。以引物F3(SEQ ID No.7)、引物R2(SEQ ID No.6)，以及以突变体cznky2020001-2各植株的DNA为模板进行PCR扩增，用来鉴定突变体cznky2020001-2各植株是否存在cznky2020001基因。将F1/R2引物对扩增后的PCR扩增产物显示约600bp，且F3/R2引物对扩增后无PCR扩增产物的植株确定为cznky2020001-2纯合突变体植株，即突变型纯合子。
3.收获突变型纯合子的种子
待所筛选出的cznky2020001-1和cznky2020001-2纯合突变体植株的种子成熟后，分别收取cznky2020001-1和cznky2020001-2纯合突变体的种子。
4.对草地贪夜蛾的抗虫试验
将纯合突变体cznky2020001-1和cznky2020001-2的种子和野生型Col-0的种子分别点铺在MS培养基上，待植株长至4-6片叶子时，分别移栽至装满蛭石的花盆中(花盆尺寸为上直径7cm，下直径5cm，高8cm)，每盆移栽5株，每个基因型移栽10盆，将植株放置在24℃，光照:黑暗＝16h:8h条件下生长21天。将草地贪夜蛾初孵幼虫接到拟南芥植株叶片上，每盆接种20只，为1个重复，每个基因型3个重复。在花盆上覆盖透气性的PVC盖子，防治虫体逃逸。分别在第7天、第10天、第12天更换新的相应基因型的拟南芥植株，保证幼虫有充足的叶片可取食。整个试验持续14天。
在整个试验期间观察幼虫的取食情况，结果发现草地贪夜蛾幼虫取食cznky2020001-1和cznky2020001-2植株的量少于野生型Col-0植株，并且取食cznky2020001-2植株的量最少，这表明cznky2020001基因的存在能够吸引草地贪夜蛾幼虫取食该植物，而与野生型对照相比，cznky2020001基因缺失的纯合突变体更不易被草地贪夜蛾幼虫取食，从而表现出抗虫性。
在第14天，取出取食cznky2020001-1、cznky2020001-2和Col-0植株的幼虫，分别单只称重，然后各处理取平均值。结果见图2。
从图2的结果可知，取食拟南芥突变型纯合子cznky2020001-1和cznky2020001-214天后的草地贪夜蛾幼虫的虫重均显著低于取食拟南芥野生型Col-0的草地贪夜蛾幼虫的虫重(P<0.005)。这进一步表明cznky2020001基因的存在能够吸引草地贪夜蛾幼虫取食该植物，进而有利于草地贪夜蛾幼虫的生长发育；而与野生型对照相比，cznky2020001基因缺失的纯合突变体更不易被草地贪夜蛾幼虫取食，进而不利于草地贪夜蛾幼虫的生长发育，从而使有关植株表现出抗虫性。
5.对甜菜夜蛾的抗虫试验
操作同第4小节，不同之处仅在于将草地贪夜蛾替换为甜菜夜蛾。
甜菜夜蛾幼虫体重的结果见图3。
在整个试验期间观察幼虫的取食情况，结果发现甜菜夜蛾幼虫取食cznky2020001-1和cznky2020001-2植株的量少于野生型Col-0植株，这表明cznky2020001基因的存在能够吸引甜菜夜蛾幼虫取食该植物，而与野生型对照相比，cznky2020001基因缺失的纯合突变体更不易被甜菜夜蛾幼虫取食，从而表现出抗虫性。
从图3的结果可知，取食拟南芥突变型纯合子cznky2020001-1和cznky2020001-214天后的甜菜夜蛾幼虫的虫重均显著低于取食拟南芥野生型Col-0的甜菜夜蛾幼虫的虫重(P<0.005)。这进一步表明cznky2020001基因的存在能够吸引甜菜夜蛾幼虫取食植物，进而有利于甜菜夜蛾幼虫的生长发育；而与野生型对照相比，cznky2020001基因缺失的纯合突变体更不易被甜菜夜蛾幼虫取食，进而不利于甜菜夜蛾幼虫的生长发育，从而表现出抗虫性。
序列表
<110> 沧州市农林科学院
<120> 一种影响鳞翅目昆虫取食的植物内源基因及其蛋白
<130> LHA2060603
<160> 7
<170> SIPOSequenceListing 1.0
<210> 1
<211> 2787
<212> DNA
<213> 拟南芥(Arabidopsis thaliana)
<400> 1
atgcttgaac caaggggagc ggacatacca atattgttcc tggttttggt tgtacttcct 60
gtggtagctt acattttatt aggaaaatgg agtaacattt ctgaaaaaag agtaagggct 120
aacttgctgg ctcagatggc agctgaagaa gctttaagag ctgagaccgt ggttaatgcg 180
gatagaggtg taagatttga gagtgtggct actgagaata gggctcaaag aaccaggacc 240
aagactgttt ctgctggtgg tggtgctgta agagcagagt ttgacgctgg tgcgagagaa 300
actgtagccg agcagagatc tgattccgtg actgctacat gtggggttac agttgtggct 360
cctgtcaata ataatgagtt acatgtatgt gcaaggtgtt ttggtcctgc taagacacgt 420
tgctcgagat gtaaatctgt tagatactgc tccgggaagt gtcaaataat tcactggagg 480
gtagctcaca aagatgaatg tgtcccagtg gagtcttgct cttcgtcatc tgagagggtt 540
tccttcgaga aagattctgt tttgtatgac catggcatgg attctacaat gtatagtaac 600
aatactacgc aggcagcaaa gggaaagact tcaaaaagct ctgtggattt tgctagcttg 660
ggtatctctc agaatgatat tacgccacaa atcaacacgc aagggaggaa aagcgtagga 720
aaacaacatt cttccaaggc caaccgagaa tcatgtagaa gagacagtgc tactgtgttt 780
gattcttctg atgaagctgc ttctgctggt ggcgataaca agacgagtca cataaaacat 840
aagtcgagag gcaacagtta tgctgcagaa acaaatccta gaaggcattc tgttgacagc 900
tctgctgtgc aaatgaatgg acaatctttt gtaagtggta tgcaggaaag tcacaaacac 960
gaaaacaatt tgggagtcag aagctctttt ggttgtccaa acacacaata tccttcaaac 1020
gggaccagaa ctgcaacact gcccagaaca ggtattaaca aatctggaga acagtcatgc 1080
acagagacaa gtaagaaggg gcaagtcgct gcagtatcaa agactgttcg gtctaaggat 1140
actggcatca gtgaagaaag caatggcatc tcctcaacga tgggaataat gaagatgatg 1200
ggtttaagaa attctacaaa gcatgatgat cgatataaga acctgaaagt tagttccctg 1260
atgctttttc catacgagga atttcttaaa ttctttcagt gtgaagtgtt tgatttatca 1320
cctagggggc ttgtaaattg tggaaacagt tgctatgcga acgctgtttt gcaatcctta 1380
acatgtacaa aaccactcgt tgcttatttg cttcgacgat cacattcaag atcatgttct 1440
gggaaagatt ggtgccttat gtgtgaactt gagcaacatg taatgatgct aagagaatcc 1500
ggaggtccac tttctgctag cagaattctc tcacacatgc gaagtataaa ttgtcagatt 1560
ggtgatggga gtcaggaaga tgctcatgag ttcttaaggc ttttggttgc ctctatgcaa 1620
tccatatgtt tggagagact tggaggtgag actaaagtgg atccgagact gcaagaaaca 1680
accttagttc aacatatgtt tggtggacgt ctccgctcaa aggttaaatg cctgaggtgt 1740
gatcatgaat cagaaagata cgagaatata atggatctca cattagagat atacggttgg 1800
gtagaatctc ttcaagatgc cttgactcag tttactagac cggaagatct cgacggagaa 1860
aacatgtata gatgcagcag gtgcgctgga tatgttagag cgaggaaaga attgagcatt 1920
catgaagcac caaacattct cacaattgtt cttaagcgat tccaggaagg aagatacggg 1980
aaaataaaca aatgtataag ttttcctgaa atgctagaca tgattccttt catgacaaga 2040
accggagacg ttcctccgct ttatatgctt tacgccgtca tagttcactt ggatactctc 2100
aacgcatctt tctcgggtca ctacatttcg tatgtcaaag atttgagagg gaattggtac 2160
agaatcgatg attcagagat tcatccagtg ccaatgactc aagttatgtc agaaggagct 2220
tacatgttgt tctacatgag atcgtatcca cgtcctcaaa gaggagagca caatggaaaa 2280
gctccggttc accattcgca accaagaaac gagatgaagg aacagaggaa acctgttaac 2340
cgcttcaaac cgagagcgga tcacaagaac acagagtcat catcaagcga atggtctctc 2400
ttcacaagct cagacgaggc ttctttcacc acagaatcaa ccagagactc tttcagcacc 2460
atagactaca cagatgtttg ccacgtcgta gactcttctt ctcctttcgc catcttcaac 2520
aacgtatacc acaacgtaga gccatcgcca cacaacactg tcgcttgcag aatgttctca 2580
ggtaccaaac ccgaaacccg gtatttcgtg gagcaagaaa caaatcacaa caacacggtc 2640
gtgctggacg caacaccatc actatatcct attccagcac cgtatccgcc ccatgattat 2700
tacgatcaga gtatgtatgt aaattacgag actaacccag aatttaacaa tggtcaggat 2760
caggatagaa cttacagtta ctggtag 2787
<210> 2
<211> 928
<212> PRT
<213> 拟南芥(Arabidopsis thaliana)
<400> 2
Met Leu Glu Pro Arg Gly Ala Asp Ile Pro Ile Leu Phe Leu Val Leu
1 5 10 15
Val Val Leu Pro Val Val Ala Tyr Ile Leu Leu Gly Lys Trp Ser Asn
20 25 30
Ile Ser Glu Lys Arg Val Arg Ala Asn Leu Leu Ala Gln Met Ala Ala
35 40 45
Glu Glu Ala Leu Arg Ala Glu Thr Val Val Asn Ala Asp Arg Gly Val
50 55 60
Arg Phe Glu Ser Val Ala Thr Glu Asn Arg Ala Gln Arg Thr Arg Thr
65 70 75 80
Lys Thr Val Ser Ala Gly Gly Gly Ala Val Arg Ala Glu Phe Asp Ala
85 90 95
Gly Ala Arg Glu Thr Val Ala Glu Gln Arg Ser Asp Ser Val Thr Ala
100 105 110
Thr Cys Gly Val Thr Val Val Ala Pro Val Asn Asn Asn Glu Leu His
115 120 125
Val Cys Ala Arg Cys Phe Gly Pro Ala Lys Thr Arg Cys Ser Arg Cys
130 135 140
Lys Ser Val Arg Tyr Cys Ser Gly Lys Cys Gln Ile Ile His Trp Arg
145 150 155 160
Val Ala His Lys Asp Glu Cys Val Pro Val Glu Ser Cys Ser Ser Ser
165 170 175
Ser Glu Arg Val Ser Phe Glu Lys Asp Ser Val Leu Tyr Asp His Gly
180 185 190
Met Asp Ser Thr Met Tyr Ser Asn Asn Thr Thr Gln Ala Ala Lys Gly
195 200 205
Lys Thr Ser Lys Ser Ser Val Asp Phe Ala Ser Leu Gly Ile Ser Gln
210 215 220
Asn Asp Ile Thr Pro Gln Ile Asn Thr Gln Gly Arg Lys Ser Val Gly
225 230 235 240
Lys Gln His Ser Ser Lys Ala Asn Arg Glu Ser Cys Arg Arg Asp Ser
245 250 255
Ala Thr Val Phe Asp Ser Ser Asp Glu Ala Ala Ser Ala Gly Gly Asp
260 265 270
Asn Lys Thr Ser His Ile Lys His Lys Ser Arg Gly Asn Ser Tyr Ala
275 280 285
Ala Glu Thr Asn Pro Arg Arg His Ser Val Asp Ser Ser Ala Val Gln
290 295 300
Met Asn Gly Gln Ser Phe Val Ser Gly Met Gln Glu Ser His Lys His
305 310 315 320
Glu Asn Asn Leu Gly Val Arg Ser Ser Phe Gly Cys Pro Asn Thr Gln
325 330 335
Tyr Pro Ser Asn Gly Thr Arg Thr Ala Thr Leu Pro Arg Thr Gly Ile
340 345 350
Asn Lys Ser Gly Glu Gln Ser Cys Thr Glu Thr Ser Lys Lys Gly Gln
355 360 365
Val Ala Ala Val Ser Lys Thr Val Arg Ser Lys Asp Thr Gly Ile Ser
370 375 380
Glu Glu Ser Asn Gly Ile Ser Ser Thr Met Gly Ile Met Lys Met Met
385 390 395 400
Gly Leu Arg Asn Ser Thr Lys His Asp Asp Arg Tyr Lys Asn Leu Lys
405 410 415
Val Ser Ser Leu Met Leu Phe Pro Tyr Glu Glu Phe Leu Lys Phe Phe
420 425 430
Gln Cys Glu Val Phe Asp Leu Ser Pro Arg Gly Leu Val Asn Cys Gly
435 440 445
Asn Ser Cys Tyr Ala Asn Ala Val Leu Gln Ser Leu Thr Cys Thr Lys
450 455 460
Pro Leu Val Ala Tyr Leu Leu Arg Arg Ser His Ser Arg Ser Cys Ser
465 470 475 480
Gly Lys Asp Trp Cys Leu Met Cys Glu Leu Glu Gln His Val Met Met
485 490 495
Leu Arg Glu Ser Gly Gly Pro Leu Ser Ala Ser Arg Ile Leu Ser His
500 505 510
Met Arg Ser Ile Asn Cys Gln Ile Gly Asp Gly Ser Gln Glu Asp Ala
515 520 525
His Glu Phe Leu Arg Leu Leu Val Ala Ser Met Gln Ser Ile Cys Leu
530 535 540
Glu Arg Leu Gly Gly Glu Thr Lys Val Asp Pro Arg Leu Gln Glu Thr
545 550 555 560
Thr Leu Val Gln His Met Phe Gly Gly Arg Leu Arg Ser Lys Val Lys
565 570 575
Cys Leu Arg Cys Asp His Glu Ser Glu Arg Tyr Glu Asn Ile Met Asp
580 585 590
Leu Thr Leu Glu Ile Tyr Gly Trp Val Glu Ser Leu Gln Asp Ala Leu
595 600 605
Thr Gln Phe Thr Arg Pro Glu Asp Leu Asp Gly Glu Asn Met Tyr Arg
610 615 620
Cys Ser Arg Cys Ala Gly Tyr Val Arg Ala Arg Lys Glu Leu Ser Ile
625 630 635 640
His Glu Ala Pro Asn Ile Leu Thr Ile Val Leu Lys Arg Phe Gln Glu
645 650 655
Gly Arg Tyr Gly Lys Ile Asn Lys Cys Ile Ser Phe Pro Glu Met Leu
660 665 670
Asp Met Ile Pro Phe Met Thr Arg Thr Gly Asp Val Pro Pro Leu Tyr
675 680 685
Met Leu Tyr Ala Val Ile Val His Leu Asp Thr Leu Asn Ala Ser Phe
690 695 700
Ser Gly His Tyr Ile Ser Tyr Val Lys Asp Leu Arg Gly Asn Trp Tyr
705 710 715 720
Arg Ile Asp Asp Ser Glu Ile His Pro Val Pro Met Thr Gln Val Met
725 730 735
Ser Glu Gly Ala Tyr Met Leu Phe Tyr Met Arg Ser Tyr Pro Arg Pro
740 745 750
Gln Arg Gly Glu His Asn Gly Lys Ala Pro Val His His Ser Gln Pro
755 760 765
Arg Asn Glu Met Lys Glu Gln Arg Lys Pro Val Asn Arg Phe Lys Pro
770 775 780
Arg Ala Asp His Lys Asn Thr Glu Ser Ser Ser Ser Glu Trp Ser Leu
785 790 795 800
Phe Thr Ser Ser Asp Glu Ala Ser Phe Thr Thr Glu Ser Thr Arg Asp
805 810 815
Ser Phe Ser Thr Ile Asp Tyr Thr Asp Val Cys His Val Val Asp Ser
820 825 830
Ser Ser Pro Phe Ala Ile Phe Asn Asn Val Tyr His Asn Val Glu Pro
835 840 845
Ser Pro His Asn Thr Val Ala Cys Arg Met Phe Ser Gly Thr Lys Pro
850 855 860
Glu Thr Arg Tyr Phe Val Glu Gln Glu Thr Asn His Asn Asn Thr Val
865 870 875 880
Val Leu Asp Ala Thr Pro Ser Leu Tyr Pro Ile Pro Ala Pro Tyr Pro
885 890 895
Pro His Asp Tyr Tyr Asp Gln Ser Met Tyr Val Asn Tyr Glu Thr Asn
900 905 910
Pro Glu Phe Asn Asn Gly Gln Asp Gln Asp Arg Thr Tyr Ser Tyr Trp
915 920 925
<210> 3
<211> 19
<212> DNA
<213> 人工序列(Artificial sequence)
<400> 3
attttgccga tttcggaac 19
<210> 4
<211> 26
<212> DNA
<213> 人工序列(Artificial sequence)
<400> 4
ctggatgaat ctgcagaaaa aagaaa 26
<210> 5
<211> 27
<212> DNA
<213> 人工序列(Artificial sequence)
<400> 5
ctttgacttt acacattctc attgttt 27
<210> 6
<211> 27
<212> DNA
<213> 人工序列(Artificial sequence)
<400> 6
ggagcagaaa caatgagaat gtgtaaa 27
<210> 7
<211> 27
<212> DNA
<213> 人工序列(Artificial sequence)
<400> 7
aatttcttaa attctttcag tgtgaag 27

Claims (8)

1. 一种植物内源蛋白在影响昆虫取食所述植物中的应用，所述蛋白的氨基酸序列如SEQ ID No. 2所示；
通过抑制所述植物内源蛋白、失活所述植物内源蛋白、沉默所述植物内源蛋白，抑制所述植物内源蛋白的表达、沉默所述植物内源蛋白的表达以及降低所述植物内源蛋白的表达量中的一种使所述植物获得对昆虫的抗性；
所述昆虫为草地贪夜蛾或甜菜夜蛾。
2.根据权利要求1所述的应用，其特征在于，所述植物为拟南芥。
3.编码如权利要求1或2所述的应用中的植物内源蛋白的核酸在影响昆虫取食所述植物中的应用；
通过对所述核酸进行突变得到突变型植物获得对昆虫的抗性；
所述突变型植物为突变型纯合子；
所述昆虫为草地贪夜蛾或甜菜夜蛾。
4.根据权利要求3所述的应用，其特征在于，所述突变为插入突变、缺失突变、移码突变中的一种。
5.根据权利要求3所述的应用，其特征在于，所述突变为插入突变或缺失突变。
6.根据权利要求3所述的应用，其特征在于，所述突变为T-DNA插入突变。
7.根据权利要求3所述的应用，其特征在于，所述植物为拟南芥。
8. 根据权利要求3至7中任意一项所述的应用，其特征在于，所述核酸的序列如SEQ IDNo. 1所示。
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