CN114506575A

CN114506575A - PCR semi-automatic detection device for pathogen detection

Info

Publication number: CN114506575A
Application number: CN202210292308.2A
Authority: CN
Inventors: 陈华; 谭淼
Original assignee: Suzhou Haimiao Biotechnology Co ltd
Current assignee: Suzhou Haimiao Biotechnology Co ltd
Priority date: 2022-03-24
Filing date: 2022-03-24
Publication date: 2022-05-17

Abstract

The invention discloses a PCR semi-automatic detection device for pathogen detection, relates to the technical field of PCR detection, and solves the problem that reagents are carelessly dumped easily when used, so that unnecessary loss is caused. A PCR semi-automatic detection device for pathogen detection comprises an outer box body; outer box body vertical slip is provided with the slider, and slider upper portion rotates and is provided with the card strip, and the fixed first support bullet piece that is provided with in slider bottom, and slider head end and support frame tail end upper portion rotate to be connected, and the support frame is two rectangle strip alternately and rotates to constitute, and support frame tail end lower part and outer box body rotate to be connected, and the upper portion and the lower part of support frame head end rotate with interior box body upper portion spout sliding connection and interior box body sub-unit connection piece respectively and are connected, go up card strip and outer box body middle part spout and run through sliding connection perpendicularly. The fixed support body of box body conduct medicament bottle in this practicality, the condition of bumping down when avoiding the medicament bottle to take.

Description

PCR semi-automatic detection device for pathogen detection

Technical Field

The invention belongs to the technical field of PCR detection, and particularly relates to a PCR semi-automatic detection device for pathogen detection.

Background

PCR detection can be used for increasing the value of genes or DNA fragments in a test tube, analyzing, detecting and identifying, is often applied to the detection of bacteria, viruses and parasites, and is widely applied to livestock breeding and the detection of human bodies.

Based on the above, the existing PCR detection reagent is placed in the reagent kit, the reagent kit is opened to take out the internal reagent when the reagent is used, the reagent kit is directly discarded when the general reagent is used, but the weight of a single reagent is light, the reagent cannot be easily poured during use, and unnecessary loss is caused.

Disclosure of Invention

In order to solve the technical problems, the invention provides a PCR semi-automatic detection device for pathogen detection, wherein an outer box body, an inner box body and an upper fixing block jointly protect an inner medicament bottle body, and the inner box body is used as a fixing frame body of the medicament bottle body, so that the situation that the medicament bottle body is knocked down when being taken and used is avoided, and the stability is enhanced.

The invention relates to a PCR semi-automatic detection device for pathogen detection, which is realized by the following specific technical means:

a PCR semi-automatic detection device for pathogen detection comprises an outer box body; the outer box body is vertically provided with a sliding block in a sliding mode, the upper portion of the sliding block is provided with an upper clamping strip in a rotating mode, the bottom of the sliding block is fixedly provided with a first supporting elastic piece, the head end of the sliding block is rotatably connected with the upper portion of the tail end of a supporting frame, the supporting frame is formed by two rectangular strips in a crossed rotating mode, the lower portion of the tail end of the supporting frame is rotatably connected with the outer box body, the upper portion and the lower portion of the head end of the supporting frame are respectively rotatably connected with an upper sliding groove of an inner box body and a lower connecting block of the inner box body in a sliding mode, the bottom end of the first supporting elastic piece is fixedly connected with the outer box body, the upper clamping strip and a middle sliding groove of the outer box body are vertically connected in a penetrating mode, the two sides of the rear portion of the inner box body are provided with supporting frames, and the upper portions of the tail end of the supporting frames on the two sides of the rear portion of the inner box body are rotatably connected with the sliding block; the middle part of the outer box body is slidably provided with an inner box body, the upper part of the inner box body is nested and slidably provided with an upper fixed block, the upper part of the inner box body is provided with an outer clamping strip in a penetrating and sliding manner, the outer clamping strip is connected with the upper end of a swinging strip in a sliding manner, the middle part of the swinging strip is connected with the inner box body in a rotating manner, the bottom end of the swinging strip is connected with a lower connecting strip in a rotating manner, the tail end of the lower connecting strip is connected with a pressing block in a rotating manner, the pressing block is connected with the inner box body in a horizontal sliding manner, a second supporting elastic piece is fixedly arranged on the inner side of the pressing block, the tail end of the second supporting elastic piece is fixed with the inner box body, the bottom of the upper fixed block is provided with a third supporting elastic piece in an array mode around the array, and the tail end of the third supporting elastic piece is fixed with the inner box body; the inside medicament bottle that is provided with of interior box body, the equal and perpendicular through sliding connection of medicament bottle in fixed block middle part on the interior box body, the equal perpendicular through sliding connection of test-tube rack in fixed block middle part on the interior box body, the test-tube rack encircles and is provided with the reagent pipe, goes up the fixed block top surface and is higher than reagent pipe and medicament bottle.

The invention at least comprises the following beneficial effects:

1. the support frame supports the inner box body to move outwards, the upper fixing block slides downwards, the use of the medicine bottle body is facilitated, the inner box body is used as a fixing frame body of the medicine bottle body, the situation that the medicine bottle body is knocked down when being taken is avoided, and the stability is enhanced.

2. The outer box body, the inner box body and the upper fixing block jointly protect the inner medicament bottle body, the protection capability on impact and collision is improved, and the isolation capability on temperature is improved.

Drawings

Fig. 1 is a schematic view of the structure of the present invention in use.

FIG. 2 is a schematic view of a half-section structure of the outer box of the present invention.

FIG. 3 is a schematic cross-sectional view of the inner case according to the present invention.

Fig. 4 is a schematic diagram of an enlarged portion a of fig. 3 according to the present invention.

FIG. 5 is a schematic view of the split structure of the present invention.

In the drawings, the corresponding relationship between the component names and the reference numbers is as follows:

1. an outer case; 101. a slider; 102. mounting a clamping strip; 103. a first supporting spring member; 104. a support frame; 2. an inner box body; 201. an upper fixed block; 202. an outer clamping strip; 203. swinging the bar; 204. a lower connecting strip; 205. according to blocks; 206. a second supporting spring; 207. a third supporting spring; 3. a medicament vial; 301. a test tube rack; 302. and a reagent tube.

Detailed Description

The embodiments of the present invention will be described in further detail with reference to the drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.

In the description of the present invention, "a plurality" means two or more unless otherwise specified; the terms "coaxial," "bottom," "one end," "top," "middle," "other end," "upper," "side," "top," "inner," "front," "center," "two ends," and the like are used in an orientation or positional relationship indicated in the drawings for convenience in describing and simplifying the description, and are not intended to indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless explicitly stated or limited otherwise, the terms "mounted," "disposed," "connected," "fixed," "screwed" and the like are to be understood broadly, and for example, they may be fixedly connected, detachably connected, or integrated; can be mechanically or electrically connected; the terms may be directly connected or indirectly connected through an intermediate, and may be communication between two elements or interaction relationship between two elements, unless otherwise specifically limited, and the specific meaning of the terms in the present invention will be understood by those skilled in the art according to specific situations.

Example (b):

as shown in figures 1 to 5:

the invention provides a PCR semi-automatic detection device for pathogen detection, which comprises an outer box body 1; the outer box body 1 is vertically provided with a slide block 101 in a sliding way, the upper part of the slide block 101 is rotationally provided with an upper clamping strip 102, the bottom of the slide block 101 is fixedly provided with a first supporting elastic piece 103, the head end of the slide block 101 is rotationally connected with the upper part of the tail end of a supporting frame 104, the supporting frame 104 is formed by two rectangular strips which are crossed and rotated, the lower part of the tail end of the supporting frame 104 is rotationally connected with an upper sliding groove of an inner box body 2 and a lower connecting block of the inner box body 2 respectively, the bottom end of the first supporting elastic piece 103 is fixedly connected with the outer box body 1, the upper clamping strip 102 is vertically connected with a middle sliding groove of the outer box body 1 in a penetrating and sliding way, the two sides of the rear part of the inner box body 2 are provided with supporting frames 104, the upper parts of the tail ends of the supporting frames 104 on the two sides of the rear part of the inner box body 2 are rotationally connected with the slide block 101, the supporting frame 104 swings to support the inner box body 2 to move outwards, and the inner box body 2 and the upper fixing block 201 move to the outer box body 1, the opening of the medicine bottle body 3 and the reagent tube 302 is convenient; the middle part of the outer box body 1 is provided with an inner box body 2 in a sliding way; interior 2 inside medicament bottles 3 that are provided with of box body, the equal and 3 perpendicular through sliding connection of medicament bottles in fixed block 201 middle part on the interior box body 2, the equal and perpendicular through sliding connection of test-tube rack 301 in fixed block 201 middle part on the interior box body 2, test-tube rack 301 encircles and is provided with reagent pipe 302, it is higher than reagent pipe 302 and medicament bottle 3 to go up fixed block 201 top surface, outer box body 1 and interior box body 2 and last fixed block 201 protect inside medicament bottle 3 and reagent pipe 302 jointly.

Referring to fig. 3 and 4, an upper fixing block 201 is slidably disposed on the upper portion of the inner case 2 in a nested manner, an outer clip strip 202 is slidably disposed on the upper portion of the inner case 2 in a penetrating manner, the outer clip strip 202 is slidably connected with the upper end of a swing strip 203, the middle portion of the swing strip 203 is rotatably connected with the inner case 2, the bottom end of the swing strip 203 is rotatably connected with a lower connecting strip 204, the tail end of the lower connecting strip 204 is rotatably connected with a pressing block 205, the inner case 2 serves as a fixing frame body of the medicine bottle 3 to prevent the medicine bottle 3 from being knocked down when being taken and used, the pressing block 205 is horizontally slidably connected with the inner case 2, a second supporting elastic member 206 is fixedly disposed on the inner side of the pressing block 205, the tail end of the second supporting elastic member 206 is fixed with the inner case 2, a third supporting elastic member 207 is disposed at the bottom of the upper fixing block 201 in a surrounding manner, the tail end of the third supporting elastic member 207 is fixed with the inner case 2, the upper fixing block 201 slides downward, a groove on the upper portion of the upper fixing block 201 is engaged with the outer clip strip 202, the lower end of the swing strip 203 swings outwards, the pressing block 205 slides outwards to protrude to the outer side of the inner box body 2, and the upper part of the medicine bottle body 3 or the test tube rack 301 leaks out, so that the medicine bottle body 3 and the reagent tube 302 can be conveniently opened for use.

The specific use mode and function of the embodiment are as follows:

in the invention, the outer box body 1, the inner box body 2 and the upper fixed block 201 jointly protect the internal medicine bottle body 3, the inner sides of the inner box body 2 and the upper fixed block 201 are provided with foaming sponge to improve the protection capability against impact and collision, the inner sides of the outer box body 1, the inner box body 2 and the upper fixed block 201 can be provided with heat-insulating foam to improve the isolation capability against temperature, the temperature rise range of the medicine bottle body 3 when being taken out of a refrigerator for use is reduced, the time limit of using and putting back the refrigerator is prolonged, the quality of internal reagents is ensured, the temperature can be kept to the maximum when the refrigerator is forgotten to be put back in time, the loss is reduced, the upper clamping strip 102 slides downwards, the upper clamping strip 102 and the sliding block 101 move downwards to compress the first supporting elastic piece 103, the supporting frame 104 swings to support the inner box body 2 to move outwards, the inner box body 2 and the upper fixed block 201 move to the outer side of the outer box body 1, the upper clamping strip 102 rotates into the lower transverse groove of the outer box body 1 to achieve the effect that the position is fixed and does not move upwards and downwards, the upper fixing block 201 slides downwards, a groove in the upper portion of the upper fixing block 201 is clamped with the outer clamping strip 202, the lower end of the swinging strip 203 swings outwards, the pressing block 205 slides outwards and protrudes to the outer side of the inner box body 2, the upper portion of the medicine bottle body 3 or the upper portion of the test tube rack 301 leaks out, the medicine bottle body 3 and the reagent tube 302 can be conveniently opened and used, the inner box body 2 serves as a fixing frame body of the medicine bottle body 3, the situation that the medicine bottle body 3 is knocked down when being taken is avoided, the stability of the medicine bottle body 3 is enhanced, the pressing block 205 is pressed, the pressing block 205 drives the swinging strip 203 to swing inwards through the lower connecting strip 204, the upper end of the swinging strip 203 swings outwards to drive the outer clamping strip 202 to slide outwards, the outer clamping strip 202 is separated from the groove in the upper portion of the upper fixing block 201, the third supporting elastic piece 207 supports the upper fixing block 201 to reset upwards, the functionality of the detection device is increased, the use experience of the device is also increased, and the market competitiveness is enhanced;

the PCR detection comprises the following operation steps:

preparing, sampling and amplifying non-transformed free nucleic acid, taking out PM-K from the kit, melting at room temperature, uniformly mixing by oscillation, taking out RE liquid, performing instantaneous centrifugation, selecting a proper centrifuge tube to prepare each PCR reaction solution, preparing a PCR reaction system as shown in the following table (n is the sample number), wherein the PM-K reaction solution is n multiplied by 19.65 mu Ln multiplied by 0.35 mu L, and subpackaging the mixed solution into n PCR reaction tubes or n reaction holes of a PCR reaction plate according to 20 mu L per tube. Transferring 5 mu L of nucleic acid to be detected (including positive control and negative control) into a reaction tube or a reaction hole, and mixing uniformly; PCR amplification apparatus setup (ABI7500 apparatus operation as an example) the reaction tube or plate was placed in the sample well of the apparatus. Opening a Setup window, clicking a Platesetup, editing a probe, selecting FAM and VIC as report groups, selecting None from an extraction and extinction group, setting a Negative Control (NC), a Positive Control (PC) and an unknown sample (Unknow) according to the corresponding sequence of the samples, and setting sample names in a column of 'SampleName'; opening an event window, and setting the circulation conditions as follows: 95 ℃ for 5 min; 95 ℃ 20sec, 60 ℃ 30sec, 72 ℃ 20 sec; the fluorescent signal collection is set at 60 ℃, the signal collection channels are FAM and VIC, and 40 cycles are carried out; after the reaction volume is set to be 25 mu L, saving a file and operating a program; solution loading and amplification of sulfite-converted free nucleic acids

Reaction solution Components

Amount of reaction solution used

Amount of RE enzyme solution

Taking out the PM-B reaction solution, the PM-N reaction solution and the PM-S reaction solution from the kit, melting at room temperature, uniformly mixing by oscillation, taking out the RE solution, and performing instantaneous centrifugation; selecting a proper centrifuge tube to prepare each PCR reaction solution, wherein the PCR reaction system is prepared as the following table (n is the number of samples):

reaction solution Components

Amount of reaction liquid

Amount of RE enzyme solution

PM-B reaction solution PM-N reaction solution PM-S reaction solution

n×19.65μLn×19.65μLn×19.65μL

n×0.35μLn×0.35μLn×0.35μL

Subpackaging the mixed solution into n PCR reaction tubes or n reaction holes of a PCR reaction plate according to 20 mu L per tube, transferring 5 mu L of nucleic acid to be detected (including positive control and negative control) into the reaction tubes or reaction holes, uniformly mixing, setting a PCR amplification instrument (ABI7500 instrument operation as an example), placing the reaction tubes or reaction plates into an instrument sample tank, opening a Setup window, clicking a PlateSetup, editing a probe, selecting FAM and VIC as report groups, selecting None as an extraction and extinction group, setting Negative Control (NC), Positive Control (PC) and unknown sample (Unknow) according to the corresponding sequence of the sample, and setting the name of the sample in the column of 'SampleName'; opening an event window, and setting the circulation conditions as follows: 95 ℃ for 5 min; 95 ℃ 20sec, 60 ℃ 30sec, 72 ℃ 20 sec; the fluorescent signal collection is set at 60 ℃, the signal collection channels are FAM and VIC, and 40 cycles are carried out; after the reaction volume was set to 25. mu.L, the file was saved and the program was run.

The invention is not described in detail, but is well known to those skilled in the art.

The embodiments of the present invention have been presented for purposes of illustration and description, and are not intended to be exhaustive or limited to the invention in the form disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiment was chosen and described in order to best explain the principles of the invention and the practical application, and to enable others of ordinary skill in the art to understand the invention for various embodiments with various modifications as are suited to the particular use contemplated.

Claims

1. A PCR semi-automatic detection device for pathogen detection is characterized in that: the PCR semi-automatic detection device for pathogen detection comprises an outer box body (1); the middle part of the outer box body (1) is provided with an inner box body (2) in a sliding way; a medicament bottle body (3) is arranged in the inner box body (2); the upper fixing block (201) is arranged on the upper portion of the inner box body (2) in a nested sliding mode, the outer clamping strip (202) is arranged on the upper portion of the inner box body (2) in a penetrating and sliding mode, the upper ends of the outer clamping strip (202) and the swinging strip (203) are connected in a sliding mode, the middle of the swinging strip (203) is connected with the inner box body (2) in a rotating mode, the bottom end of the swinging strip (203) is connected with the lower connecting strip (204) in a rotating mode, and the tail end of the lower connecting strip (204) is connected with the pressing block (205) in a rotating mode.

2. The PCR semi-automatic detection device for pathogen detection according to claim 1, wherein the outer case (1) is vertically slidably provided with a slide block (101), the upper portion of the slide block (101) is rotatably provided with an upper clamping strip (102), the bottom of the slide block (101) is fixedly provided with a first supporting elastic member (103), and the head end of the slide block (101) is rotatably connected with the tail end of the supporting frame (104).

3. The PCR semi-automatic detection device for pathogen detection according to claim 2, wherein the support frame (104) is formed by two rectangular strips which are crossed and rotated, the tail end of the support frame (104) is rotatably connected with the outer case (1), and the head end of the support frame (104) is respectively connected with the upper part of the inner case (2) in a sliding manner and the lower part of the inner case (2) in a rotating manner.

4. The PCR semi-automatic detection device for pathogen detection according to claim 2, wherein the bottom end of the first supporting elastic member (103) is fixedly connected with the outer box body (1), the upper clamping strip (102) is vertically connected with the middle part of the outer box body (1) in a sliding manner, the two sides of the rear part of the inner box body (2) are respectively provided with a supporting frame (104), and the upper parts of the tail ends of the supporting frames (104) at the two sides of the rear part of the inner box body (2) are respectively connected with the sliding block (101) in a rotating manner.

5. The PCR semi-automatic detection device for pathogen detection according to claim 1, wherein the block (205) is horizontally connected with the inner box (2) in a sliding manner, a second supporting spring (206) is fixedly arranged on the inner side of the block (205), the tail end of the second supporting spring (206) is fixed with the inner box (2), a third supporting spring (207) is arranged at the bottom of the upper fixing block (201) in a surrounding manner, and the tail end of the third supporting spring (207) is fixed with the inner box (2).

6. The PCR semi-automatic detection device for pathogen detection according to claim 5, wherein the middle parts of the upper fixing blocks (201) of the inner box body (2) are connected with the reagent bottles (3) in a penetrating and sliding manner, and the middle parts of the upper fixing blocks (201) of the inner box body (2) are connected with the test tube racks (301) in a penetrating and sliding manner.

7. The PCR semi-automatic detection device for pathogen detection according to claim 6, wherein the test tube rack (301) is surrounded by the reagent tube (302), and the top surface of the upper fixing block (201) is higher than the reagent tube (302) and the reagent bottle (3).