CN114486433A - Animal tissue culture processing treatment device - Google Patents
Animal tissue culture processing treatment device Download PDFInfo
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- CN114486433A CN114486433A CN202210081071.3A CN202210081071A CN114486433A CN 114486433 A CN114486433 A CN 114486433A CN 202210081071 A CN202210081071 A CN 202210081071A CN 114486433 A CN114486433 A CN 114486433A
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
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- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2866—Grinding or homogeneising
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2873—Cutting or cleaving
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Abstract
The invention belongs to the technical field of animal tissue culture, and particularly relates to an animal tissue culture processing device which comprises a substrate, wherein a driving motor is arranged on the lower surface of the substrate, a main shaft is fixedly arranged at the output end of the driving motor, a supporting cylinder is fixedly arranged on the upper surface of the substrate, and a processing cylinder is fixedly arranged at the top of the supporting cylinder; the outer wall of the main shaft is sleeved with a sleeve shaft, and a bearing ring is fixedly arranged on the main shaft; the fixed cover of cover epaxial outer wall is equipped with the ring of grinding, installs the square cover of a plurality of on the main shaft outer wall, all installs the mechanism of milling on every square cover. When the animal tissue is subjected to crushing processing treatment, the animal tissue is subjected to bidirectional grinding in the horizontal and vertical directions by the grinding strip and the grinding ring; meanwhile, the first cutting knife and the second cutting knife cut the animal tissues in the horizontal and vertical directions, so that the breaking effect on fascia and muscle fibers in the animal tissues is improved.
Description
Technical Field
The invention belongs to the technical field of animal tissue culture, and particularly relates to an animal tissue culture processing device.
Background
Animal tissue culture is a process of extracting tissue from an animal, and incubating and culturing the tissue in a specific in vitro environment such as a simulated in vivo physiological environment to enable the tissue to survive and grow. When the method is used, animal tissue needs to be extracted firstly, the animal tissue is crushed and then treated by trypsin to form dispersed single cells, and the treated cells are transplanted into a culture medium to prepare a cell suspension with a certain concentration for processing. The cells dispersed in the suspension are attached to the wall of the bottle, when adherent cells grow until the adherent cells are contacted with each other, the cells stop division and proliferation, contact inhibition occurs, at the moment, the cells with contact inhibition are treated by trypsin again, and then cell suspension with a certain concentration is prepared for processing.
At present, animal tissues are crushed mainly in a mode that a grinding block rotates and grinds around a grinding ring, the grinding direction is single, fascia and muscle fibers in the animal tissues are difficult to be fully crushed, so that the crushed tissues are difficult to be fully dispersed into single cells in the trypsin treatment process, and the culture effect is influenced; the existing milling device is complex in internal structure, dead angles exist, the milling block is inconvenient to disassemble, and the interior of the processing device is difficult to clean fully.
Disclosure of Invention
In order to solve the technical problems, the invention adopts the following technical scheme: an animal tissue culture processing device comprises a horizontal base plate, wherein a plurality of supporting legs are fixedly arranged on the lower surface of the base plate, a driving motor is fixedly arranged in the middle of the lower surface of the base plate, a main shaft vertically penetrating through the base plate is fixedly arranged at the output end of the driving motor, a supporting cylinder is fixedly arranged on the upper surface of the base plate, and a processing cylinder which is superposed with the axis of the main shaft is fixedly arranged at the top of the supporting cylinder; the outer wall of the main shaft is sleeved with a sleeve shaft which is coincident with the axis of the main shaft, a bearing ring is fixedly arranged at the position of the main shaft below the sleeve shaft, the bottom surface of the sleeve shaft is attached to the upper surface of the bearing ring, and the main shaft and the sleeve shaft penetrate through the bottom plate of the processing cylinder.
A grinding ring which is coincident with the axis of the sleeve shaft is fixedly sleeved on the outer wall of the sleeve shaft, and the bottom surface of the grinding ring is attached to the bottom surface of the interior of the processing cylinder; a plurality of square sleeves are uniformly arranged on the outer wall of the main shaft above the sleeve shaft along the circumferential direction of the main shaft, and each square sleeve is provided with a grinding mechanism; the grinding mechanism comprises a horizontal arm which is movably inserted on the square sleeve, a lifting rod which vertically penetrates through the horizontal arm is installed on the horizontal arm in a sliding fit mode, a vertical grinding strip is fixedly installed at the bottom end of the lifting rod, the surface of the grinding strip, facing the grinding ring, is an arc surface, and the top surface of the grinding strip is connected with the lower surface of the horizontal arm through an elastic telescopic rod; a ball seat is fixedly arranged at the top end of the lifting rod, and a first rolling ball is arranged on the top surface of the ball seat; the apron is installed through the detachable mode at a processing section of thick bamboo top, and a plurality of second spin is evenly installed along main shaft circumference to the apron lower surface, and the distance between first spin and the main shaft equals the distance between second spin and the main shaft.
As a preferred technical scheme of the invention, the main shaft is in rotating fit with the sleeve shaft, the outer wall of the main shaft is fixedly sleeved with a first bevel gear, the outer wall of the sleeve shaft is fixedly sleeved with a second bevel gear, the base plate is fixedly provided with a gear carrier, and the gear carrier is rotatably provided with a transmission bevel gear which is meshed with the first bevel gear and the second bevel gear.
As a preferable technical scheme of the invention, the bottom surface of the sleeve shaft is rotatably provided with the ball, and the ball is attached to the upper surface of the bearing ring.
According to a preferable technical scheme of the invention, two vertical strip-shaped grooves are formed in the arc-shaped surface of the grinding strip facing the grinding ring, feeding shafts penetrating through the strip-shaped grooves are rotatably mounted in the strip-shaped grooves, a plurality of vertical feeding plates are uniformly and fixedly mounted on the feeding shafts along the circumferential direction of the feeding shafts, feeding motors are fixedly mounted on the top surfaces of the grinding strips corresponding to the positions of the feeding shafts, and the output ends of the feeding motors are fixedly connected with the top ends of the feeding shafts.
As a preferred technical scheme of the invention, a plurality of annular grooves are uniformly formed in the outer wall of the main shaft from top to bottom, a guide ring is fixedly arranged in each annular groove, and the outer circumferential surface of each guide ring is wavy; two horizontal through grooves are symmetrically formed in the sleeve shaft corresponding to the positions of the annular grooves, sliding blocks are horizontally and movably mounted in the through grooves, first cutting knives penetrating through the grinding rings are horizontally and fixedly mounted on the end faces, away from the main shaft, of the sliding blocks, horizontal reset springs are fixedly connected between the sliding blocks and the grinding rings, rollers are rotatably mounted on the end faces, facing the main shaft, of the sliding blocks through roller frames, and the rollers are attached to the outer circumferential surfaces of the guide rings.
As a preferred technical scheme of the invention, a vertical cutter groove is formed in the outer wall of the grinding ring at the position between two adjacent first cutters in the horizontal direction, and a vertical second cutter is horizontally and movably arranged in the cutter groove; a horizontal compression spring is fixedly connected between the second cutting knife and the inner wall of the cutter groove, Contraband-shaped grooves are formed in the inner part of the grinding ring corresponding to each cutter groove, one port of the Contraband-shaped groove is positioned on the inner wall of the cutter groove, a horizontal guide rod is slidably mounted at the port, and the end part of the guide rod is fixedly connected with the second cutting knife; the other port of the Contraband-shaped groove is positioned on the outer wall of the grinding ring and is provided with a balancing weight in a sliding way, and the balancing weight is connected with the guide rod through a connecting rope.
As a preferable technical scheme of the invention, the surface of the balancing weight is provided with a rotating roller which is matched with the inner wall of the v-shaped groove of 21274.
As a preferred technical scheme of the invention, a positioning rod vertically penetrating through the square sleeve is arranged on the square sleeve in a sliding fit mode, and a positioning groove matched with the positioning rod is formed in the horizontal arm; the top end of the main shaft is provided with an accommodating groove, a position on the inner side wall of the accommodating groove corresponding to each positioning rod is horizontally and slidably provided with a limiting rod, and the positioning rods are provided with limiting grooves matched with the limiting rods; a pressure-bearing ring is fixedly sleeved on the limiting rod, and a horizontal limiting spring is fixedly connected between the pressure-bearing ring and the inner side wall of the accommodating groove; install solid fixed ring on the holding tank inner wall, gu fixed ring surface fixed mounting has the vertical connecting spring of a plurality of, the lateral wall is the guide block on inclined plane is installed jointly to the vertical connecting spring bottom of a plurality of, and terminal surface and guide block lateral wall are laminated mutually in the gag lever post, and guide block top fixed mounting has the pull rod.
The invention has at least the following beneficial effects: (1) when the animal tissue is crushed and processed by the invention, the grinding strip reciprocates up and down along the axial direction of the grinding ring while rotating around the grinding ring in a reciprocating way, so that the animal tissue between the grinding ring and the grinding strip is subjected to bidirectional grinding in the horizontal and vertical directions, and the crushing effect on fascia and muscle fibers in the animal tissue is improved; simultaneously, at the strip of milling around grinding ring pivoted in-process, the first cutting knife of horizontality carries out the horizontal direction cutting to animal tissue, grinds the strip by rotating to static to the in-process of antiport again, and the second is cut to carrying out the cutting of vertical direction to animal tissue, has further improved the crushing effect to the manadesma in the animal tissue and muscle fibre.
(2) The grinding mechanism can be integrally disassembled from the square sleeve, and the positioning rod can be removed after the lifting rod is manually moved upwards during disassembly, so that the operation is convenient; after the grinding mechanism is manually disassembled, only one annular groove formed by the inner wall of the processing cylinder and the outer wall of the grinding ring is formed in the processing cylinder, so that dead corners do not exist, and the interior of the processing cylinder is convenient to clean.
Drawings
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 is a schematic perspective view of an apparatus for processing and culturing animal tissue according to an embodiment of the present invention.
FIG. 2 is a front view showing the internal structure of the animal tissue culture processing device according to the embodiment of the present invention.
Fig. 3 is an enlarged schematic view of a point a in fig. 2.
Fig. 4 is an enlarged schematic view of fig. 2 at B.
Figure 5 is a side view of a portion of the internal structure of a grinding ring and sleeve shaft in an embodiment of the present invention.
Fig. 6 is an enlarged schematic view at C in fig. 5.
Fig. 7 is a schematic perspective view of a grinding mechanism according to an embodiment of the present invention.
Fig. 8 is a schematic perspective view of a spindle according to an embodiment of the present invention.
In the figure: 1. a substrate; 2. a drive motor; 3. a main shaft; 301. an annular groove; 302. accommodating grooves; 4. a support cylinder; 5. processing the cylinder; 6. a sleeve shaft; 601. a through groove; 7. grinding the ring; 701. a cutter groove; 702. contraband-shaped grooves; 8. a square sleeve; 9. a milling mechanism; 901. a horizontal arm; 902. a lifting rod; 903. grinding the strips; 904. an elastic telescopic rod; 905. a ball seat; 906. a first ball; 907. a strip-shaped groove; 908. a feed shaft; 909. a feeding plate; 910. a feeding motor; 911. positioning a groove; 10. a cover plate; 11. a second roller ball; 12. a first bevel gear; 13. a second bevel gear; 14. a drive bevel gear; 15. a ball bearing; 16. a guide ring; 17. a slider; 18. a first cutter; 19. a return spring; 20. a roller; 21. a second cutter; 22. a compression spring; 23. a guide bar; 24. a balancing weight; 25. connecting ropes; 26. a rotating roller; 27. positioning a rod; 2701. a limiting groove; 28. a limiting rod; 29. a pressure-bearing ring; 30. a limiting spring; 31. a fixing ring; 32. a connecting spring; 33. a guide block; 34. and lifting the pull rod.
Detailed Description
The embodiments of the invention will be described in detail below with reference to the drawings, but the invention can be implemented in many different ways as defined and covered by the claims.
As shown in fig. 1 and fig. 2, the present embodiment provides an animal tissue culture processing device, including a horizontal substrate 1, a plurality of supporting legs are fixedly mounted on a lower surface of the substrate 1, a driving motor 2 is fixedly mounted in a middle portion of the lower surface of the substrate 1, the driving motor 2 is a reciprocating motor, a spindle 3 vertically penetrating through the substrate 1 is fixedly mounted at an output end of the driving motor 2, a supporting cylinder 4 is fixedly mounted on an upper surface of the substrate 1, and a processing cylinder 5 coinciding with an axis of the spindle 3 is fixedly mounted at a top of the supporting cylinder 4; a sleeve shaft 6 in rotating fit with the main shaft 3 is sleeved on the outer wall of the main shaft 3, a bearing ring is fixedly arranged at the position, below the sleeve shaft 6, of the main shaft 3, a ball 15 is rotatably arranged on the bottom surface of the sleeve shaft 6, and the ball 15 is attached to the upper surface of the bearing ring; the main shaft 3 and the sleeve shaft 6 both penetrate through the bottom plate of the processing cylinder 5; a first bevel gear 12 is fixedly sleeved on the outer wall of the main shaft 3, a second bevel gear 13 is fixedly sleeved on the outer wall of the sleeve shaft 6, a gear carrier is fixedly installed on the substrate 1, and a transmission bevel gear 14 meshed with the first bevel gear 12 and the second bevel gear 13 is rotatably installed on the gear carrier.
As shown in fig. 2, 3 and 7, a grinding ring 7 coinciding with the axis of the sleeve shaft 6 is fixedly sleeved on the outer wall of the sleeve shaft 6, and the bottom surface of the grinding ring 7 is attached to the bottom surface of the inner part of the processing cylinder 5; a plurality of square sleeves 8 are uniformly arranged on the outer wall of the main shaft 3 above the sleeve shaft 6 along the circumferential direction of the main shaft 3, and each square sleeve 8 is provided with a grinding mechanism 9; the grinding mechanism 9 comprises a horizontal arm 901 movably inserted on the square sleeve 8, a lifting rod 902 vertically penetrating through the horizontal arm 901 is installed on the horizontal arm 901 in a sliding fit manner, a vertical grinding strip 903 is fixedly installed at the bottom end of the lifting rod 902, the surface of the grinding strip 903 facing the grinding ring 7 is an arc surface, and the top surface of the grinding strip 903 is connected with the lower surface of the horizontal arm 901 through an elastic telescopic rod 904; a ball seat 905 is fixedly arranged at the top end of the lifting rod 902, and a first rolling ball 906 is arranged on the top surface of the ball seat 905; the top of the processing cylinder 5 is detachably provided with a cover plate 10, the lower surface of the cover plate 10 is uniformly provided with a plurality of second rolling balls 11 along the circumferential direction of the main shaft 3, and the distance between the first rolling balls 906 and the main shaft 3 is equal to the distance between the second rolling balls 11 and the main shaft 3; two vertical strip grooves 907 are formed in the arc-shaped surface, facing the milling ring 7, of the milling strip 903, the feeding shafts 908 penetrating through the strip grooves 907 are rotatably mounted in the strip grooves 907, a plurality of vertical feeding plates 909 are uniformly and fixedly mounted on the feeding shafts 908 along the circumferential direction of the feeding shafts, the positions, corresponding to the feeding shafts 908, of the top surface of the milling strip 903 are fixedly provided with feeding motors 910, and the output ends of the feeding motors 910 are fixedly connected with the top ends of the feeding shafts 908.
The main shaft 3 is driven to rotate by the driving motor 2, the main shaft 3 drives the first bevel gear 12, the square sleeve 8 and the grinding mechanism 9 to rotate, the first bevel gear 12 drives the second bevel gear 13 to rotate by the transmission bevel gear 14, and the sleeve shaft 6 and the main shaft 3 are driven to rotate in opposite directions by the second bevel gear 13, so that the grinding ring 7 and the grinding mechanism 9 rotate in opposite directions; in the process, animal tissues enter between the grinding ring 7 and the grinding strip 903 and are crushed under the action of extrusion force, and as the grinding mechanism 9 continuously rotates, after the first rolling ball 906 and the second rolling ball 11 are attached to each other, the first rolling ball 906, the ball seat 905, the lifting rod 902, the grinding strip 903, the feeding shaft 908, the feeding plate 909 and the feeding motor 910 integrally move downwards, the elastic telescopic rod 904 stretches, and the grinding strip 903 moves downwards relative to the grinding ring 7; when the first rolling ball 906 is separated from the second rolling ball 11, the first rolling ball 906, the ball seat 905, the lifting rod 902, the grinding strip 903, the feeding shaft 908, the feeding plate 909 and the feeding motor 910 are integrally reset upwards, and the grinding strip 903 moves upwards relative to the grinding ring 7, so that the effect of grinding animal tissues horizontally and vertically in opposite directions is realized; in the above process, the feeding shaft 908 and the feeding plate 909 are driven by the feeding motor 910 to rotate continuously, and the feeding plate 909 pushes the animal tissue into the gap between the grinding strip 903 and the grinding ring 7, so as to improve the grinding effect.
As shown in fig. 3, a positioning rod 27 vertically penetrating through the square sleeve 8 is installed on the square sleeve 8 in a sliding fit manner, and a positioning groove 911 matched with the positioning rod 27 is formed on the horizontal arm 901; the top end of the spindle 3 is provided with an accommodating groove 302, the position of the inner side wall of the accommodating groove 302 corresponding to each positioning rod 27 is horizontally and slidably provided with a limiting rod 28, and the positioning rod 27 is provided with a limiting groove 2701 matched with the limiting rod 28; a pressure-bearing ring 29 is fixedly sleeved on the limiting rod 28, and a horizontal limiting spring 30 is fixedly connected between the pressure-bearing ring 29 and the inner side wall of the accommodating groove 302; install solid fixed ring 31 on the holding tank 302 inner wall, gu fixed ring 31 lower fixed surface installs the vertical connecting spring 32 of a plurality of, the lateral wall is installed jointly to the vertical connecting spring 32 bottom of a plurality of and is the guide block 33 on inclined plane, and the terminal surface is laminated with the guide block 33 lateral wall mutually in the gag lever post 28, and guide block 33 top fixed mounting has lifting rod 34.
When the grinding mechanism 9 is disassembled, the lifting rod 34 is moved upwards manually to drive the guide block 33 to overcome the elastic force of the connecting spring 32, the pressure-bearing ring 29 and the limiting rod 28 move towards the inner side of the accommodating groove 302 by the elastic force of the limiting spring 30 until the limiting rod 28 moves out of the limiting groove 2701, the positioning rod 27 can be disassembled under the state, then the grinding mechanism 9 can be integrally disassembled from the square sleeve 8, the disassembling operation is convenient, and the grinding mechanism 9 is ensured not to fall off from the square sleeve 8 in the working process.
As shown in fig. 4 and 8, a plurality of annular grooves 301 are uniformly formed in the outer wall of the main shaft 3 from top to bottom, a guide ring 16 is fixedly installed in each annular groove 301, and the outer circumferential surface of each guide ring 16 is wavy; two horizontal through grooves 601 are symmetrically formed in the sleeve shaft 6 corresponding to the positions of the annular grooves 301, a sliding block 17 is horizontally and movably mounted in the through grooves 601, a first cutting knife 18 penetrating through the grinding ring 7 is horizontally and fixedly mounted on the end face, away from the main shaft 3, of the sliding block 17, a horizontal reset spring 19 is fixedly connected between the sliding block 17 and the grinding ring 7, an idler wheel 20 is rotatably mounted on the end face, facing the main shaft 3, of the sliding block 17 through an idler wheel frame, and the idler wheel 20 is attached to the outer circumferential surface of the guide ring 16.
During the rotation of the sleeve shaft 6, the roller 20 is always attached to and rolls on the outer circumferential surface of the guide ring 16, and because the outer circumferential surface of the guide ring 16 is wavy, the slider 17, the first cutter 18 and the roller 20 integrally move horizontally in a reciprocating manner under the guiding action of the guide ring 16 and the resilience force of the return spring 19, and the first cutter 18 periodically extends out of the outer surface of the grinding ring 7 during the horizontal reciprocating movement to cut animal tissues in the horizontal direction.
As shown in fig. 5 and 6, a vertical cutter groove 701 is formed in the outer wall of the grinding ring 7 at a position between two adjacent first cutting knives 18 in the horizontal direction, and a vertical second cutting knife 21 is horizontally and movably mounted in the cutter groove 701; a horizontal compression spring 22 is fixedly connected between the second cutting knife 21 and the inner wall of the cutter groove 701, Contraband-shaped grooves 702, 21274are formed in the positions, corresponding to the cutter grooves 701, in the grinding ring 7, one port of each shaped groove 702 is positioned on the inner wall of the cutter groove 701, a horizontal guide rod 23 is slidably mounted at the port, and the end part of each guide rod 23 is fixedly connected with the second cutting knife 21; contraband the other end of the groove 702 is located on the outer wall of the grinding ring 7 and the end is mounted with the counterweight 24 in a sliding way, the counterweight 24 is connected with the guide rod 23 through the connecting rope 25; the surface of the counter weight block 24 is provided with a rotating roller 26, and the rotating roller 26 is matched with the inner wall of the v-shaped groove 702 in a rolling way; it should be noted that the outer edge of the second cutting knives 21, i.e. the edge of the knives, does not contact the milling bar 903.
In the rotation process of the sleeve shaft 6 and the grinding ring 7, the second cutting knife 21, the compression spring 22, the guide rod 23, the balancing weight 24, the connecting rope 25 and the rotating roller 26 synchronously rotate, the balancing weight 24 horizontally moves outwards under the action of centrifugal force, the connecting rope 25 is tensioned by the balancing weight 24, then the guide rod 23 is pulled to horizontally move inwards, the second cutting knife 21 moves towards the inside of the knife groove 701, and the compression spring 22 is compressed; because the driving motor 2 is a bidirectional motor, when the driving motor 2 is reversed, the sleeve shaft 6 and the grinding ring 7 rotate to be static and then rotate reversely, the centrifugal force of the balancing weight 24 is gradually reduced and then increased, and in the process that the centrifugal force of the balancing weight 24 is reduced, the resilience force of the compression spring 22 enables the second cutting knife 21 to move towards the outside of the knife slot 701, and the second cutting knife 21 moves out of the outer side wall of the grinding ring 7 to cut animal tissues in the vertical direction; the purpose of the above structure is to avoid the generation of squeezing between the second cutting knife 21 and the animal tissue during the rotation of the sleeve shaft 6 and the grinding ring 7, and the second cutting knife 21 only cuts the animal tissue once during the rest of the sleeve shaft 6 and the grinding ring 7.
The working process of the animal tissue culture processing device in the embodiment is as follows: animal tissues are manually placed into the processing cylinder 5, the main shaft 3 is driven to rotate through the driving motor 2, and the grinding ring 7 and the grinding strip 903 rotate in the opposite direction and move in a reciprocating manner in the vertical direction; in the process, animal tissues enter between the grinding ring 7 and the grinding strip 903 and are crushed under the action of extrusion force, the feeding motor 910 drives the feeding shaft 908 and the feeding plate 909 to rotate continuously, and the feeding plate 909 pushes the animal tissues into a gap between the grinding strip 903 and the grinding ring 7 so as to improve the grinding effect; in the rotation process of the sleeve shaft 6, the first cutting knife 18 periodically extends out of the outer surface of the grinding ring 7 to cut animal tissues in the horizontal direction; the second cutting knife 21 makes a single cut of the animal tissue during the relative rest of the milling bar 903 and the milling ring 7; when the grinding mechanism 9 is disassembled, the lifting rod 34 is manually moved upwards to drive the guide block 33 to overcome the elasticity of the connecting spring 32 to rise, then the positioning rod 27 is disassembled, and finally the grinding mechanism 9 is integrally disassembled from the square sleeve 8.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. The utility model provides an animal tissue culture processing apparatus, includes horizontally base plate (1), and fixed surface installs a plurality of supporting leg, its characterized in that under base plate (1): the middle part of the lower surface of the base plate (1) is fixedly provided with a driving motor (2), the output end of the driving motor (2) is fixedly provided with a main shaft (3) which vertically penetrates through the base plate (1), the upper surface of the base plate (1) is fixedly provided with a supporting cylinder (4), and the top of the supporting cylinder (4) is fixedly provided with a processing cylinder (5) which is superposed with the axis of the main shaft (3); a sleeve shaft (6) which is coincident with the axis of the main shaft is sleeved on the outer wall of the main shaft (3), a bearing ring is fixedly installed at the position, below the sleeve shaft (6), of the main shaft (3), the bottom surface of the sleeve shaft (6) is attached to the upper surface of the bearing ring, and the main shaft (3) and the sleeve shaft (6) penetrate through a bottom plate of the processing cylinder (5);
a grinding ring (7) which is coincident with the axis of the sleeve shaft (6) is fixedly sleeved on the outer wall of the sleeve shaft, and the bottom surface of the grinding ring (7) is attached to the inner bottom surface of the processing cylinder (5); a plurality of square sleeves (8) are uniformly arranged on the outer wall of the main shaft (3) above the sleeve shaft (6) along the circumferential direction of the main shaft (3), and each square sleeve (8) is provided with a grinding mechanism (9); the grinding mechanism (9) comprises a horizontal arm (901) movably inserted on the square sleeve (8), a lifting rod (902) vertically penetrating through the horizontal arm (901) is installed on the horizontal arm (901) in a sliding fit mode, a vertical grinding strip (903) is fixedly installed at the bottom end of the lifting rod (902), the surface, facing the grinding ring (7), of the grinding strip (903) is an arc-shaped surface, and the top surface of the grinding strip (903) is connected with the lower surface of the horizontal arm (901) through an elastic telescopic rod (904); a ball seat (905) is fixedly arranged at the top end of the lifting rod (902), and a first rolling ball (906) is arranged on the top surface of the ball seat (905); a cover plate (10) is installed at the top of the processing barrel (5) in a detachable mode, a plurality of second rolling balls (11) are evenly installed on the lower surface of the cover plate (10) along the circumferential direction of the main shaft (3), and the distance between the first rolling balls (906) and the main shaft (3) is equal to the distance between the second rolling balls (11) and the main shaft (3).
2. The animal tissue culture processing device of claim 1, wherein: the main shaft (3) is in running fit with the sleeve shaft (6), a first bevel gear (12) is fixedly sleeved on the outer wall of the main shaft (3), a second bevel gear (13) is fixedly sleeved on the outer wall of the sleeve shaft (6), a gear carrier is fixedly mounted on the substrate (1), and a transmission bevel gear (14) which is meshed with the first bevel gear (12) and the second bevel gear (13) is rotatably mounted on the gear carrier.
3. The animal tissue culture processing device of claim 1, wherein: the bottom surface of the sleeve shaft (6) is rotatably provided with a ball (15), and the ball (15) is attached to the upper surface of the bearing ring.
4. The animal tissue culture processing device of claim 1, wherein: the milling strip (903) is provided with two vertical strip grooves (907) towards the arc-shaped surface of the milling ring (7), a feeding shaft (908) penetrating through the strip grooves (907) is installed in the strip grooves (907) in a rotating mode, a plurality of vertical feeding plates (909) are evenly and fixedly installed on the feeding shaft (908) along the circumferential direction of the feeding shaft, a feeding motor (910) is fixedly installed on the top surface of the milling strip (903) corresponding to the position of each feeding shaft (908), and the output end of the feeding motor (910) is fixedly connected with the top end of the feeding shaft (908).
5. The animal tissue culture processing device of claim 1, wherein: a plurality of annular grooves (301) are uniformly formed in the outer wall of the main shaft (3) from top to bottom, a guide ring (16) is fixedly installed in each annular groove (301), and the outer circumferential surface of each guide ring (16) is wavy; two horizontal through grooves (601) are formed in the position symmetry corresponding to each annular groove (301) on the sleeve shaft (6), a sliding block (17) is movably mounted in the through grooves (601), a first cutting knife (18) penetrating through the grinding ring (7) is horizontally and fixedly mounted on the end face, far away from the main shaft (3), of the sliding block (17), a horizontal reset spring (19) is fixedly connected between the sliding block (17) and the grinding ring (7), an idler wheel (20) is rotatably mounted on the end face, facing the main shaft (3), of the sliding block (17) through an idler wheel frame, and the idler wheel (20) is attached to the outer circumferential face of the guide ring (16).
6. The animal tissue culture processing device of claim 5, wherein: a vertical cutter groove (701) is formed in the outer wall of the grinding ring (7) and positioned between two adjacent first cutters (18) in the horizontal direction, and a vertical second cutter (21) is horizontally and movably arranged in the cutter groove (701); a horizontal compression spring (22) is fixedly connected between the second cutting knife (21) and the inner wall of the cutter groove (701), an Contraband-shaped groove (702), a v-shaped guide rod (23) is arranged at a position, corresponding to each cutter groove (701), in the grinding ring (7), one port of the v-shaped groove (702) is positioned on the inner wall of the cutter groove (701), a horizontal guide rod (23) is slidably installed at the port, and the end part of the guide rod (23) is fixedly connected with the second cutting knife (21); the other port of the Contraband-shaped groove (702) is positioned on the outer wall of the grinding ring (7), a balancing weight (24) is installed at the port in a sliding way, and the balancing weight (24) is connected with the guide rod (23) through a connecting rope (25).
7. The animal tissue culture processing device of claim 6, wherein: and a rotating roller (26) is arranged on the surface of the balancing weight (24), and the rotating roller (26) is in rolling fit with the inner wall of the Contraband-shaped groove (702).
8. The animal tissue culture processing device of claim 1, wherein: a positioning rod (27) vertically penetrating through the square sleeve (8) is installed on the square sleeve (8) in a sliding fit mode, and a positioning groove (911) matched with the positioning rod (27) is formed in the horizontal arm (901); the top end of the main shaft (3) is provided with a containing groove (302), the position of the inner side wall of the containing groove (302) corresponding to each positioning rod (27) is horizontally and slidably provided with a limiting rod (28), and the positioning rod (27) is provided with a limiting groove (2701) matched with the limiting rod (28); a pressure-bearing ring (29) is fixedly sleeved on the limiting rod (28), and a horizontal limiting spring (30) is fixedly connected between the pressure-bearing ring (29) and the inner side wall of the accommodating groove (302); install solid fixed ring (31) on holding tank (302) inner wall, gu fixed ring (31) lower fixed surface installs vertical connecting spring of a plurality of (32), guide block (33) that the lateral wall is the inclined plane are installed jointly to vertical connecting spring of a plurality of (32) bottom, and the terminal surface is laminated with guide block (33) lateral wall mutually in gag lever post (28), and guide block (33) top fixed mounting has lifting rod (34).
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210081071.3A CN114486433A (en) | 2022-01-24 | 2022-01-24 | Animal tissue culture processing treatment device |
| PCT/CN2022/144051 WO2023138347A1 (en) | 2022-01-24 | 2022-12-30 | Animal tissue culture processing apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210081071.3A CN114486433A (en) | 2022-01-24 | 2022-01-24 | Animal tissue culture processing treatment device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114486433A true CN114486433A (en) | 2022-05-13 |
Family
ID=81474695
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210081071.3A Withdrawn CN114486433A (en) | 2022-01-24 | 2022-01-24 | Animal tissue culture processing treatment device |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN114486433A (en) |
| WO (1) | WO2023138347A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023138347A1 (en) * | 2022-01-24 | 2023-07-27 | 阜阳师范大学 | Animal tissue culture processing apparatus |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102009006917A1 (en) * | 2009-01-30 | 2010-08-12 | Alfers, Harald, S., Dipl.-Ing. | Device for crushing at least one material to be ground |
| CN101518751A (en) * | 2009-02-13 | 2009-09-02 | 黄琳 | Combined crusher |
| DE202014105347U1 (en) * | 2014-11-07 | 2014-11-25 | GEFU-Küchenboss GmbH & Co. KG | Hand mill for crushing regrind |
| WO2017152334A1 (en) * | 2016-03-07 | 2017-09-14 | 刘湘静 | New and highly efficient grain milling device |
| DE102016216345B3 (en) * | 2016-08-30 | 2018-01-25 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Apparatus and method for the dissociation of tissue |
| CN111886990B (en) * | 2020-08-12 | 2022-03-15 | 潍坊学院 | Daily fertilization processing apparatus of farming |
| CN112871300A (en) * | 2021-01-12 | 2021-06-01 | 南京楠凡钰商贸有限公司 | Preparation method of aloe plant natural extract |
| CN112871382A (en) * | 2021-01-12 | 2021-06-01 | 南京楠凡钰商贸有限公司 | Aloe plant natural extract preparation is with system of processing |
| CN114486433A (en) * | 2022-01-24 | 2022-05-13 | 阜阳师范大学 | Animal tissue culture processing treatment device |
-
2022
- 2022-01-24 CN CN202210081071.3A patent/CN114486433A/en not_active Withdrawn
- 2022-12-30 WO PCT/CN2022/144051 patent/WO2023138347A1/en unknown
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023138347A1 (en) * | 2022-01-24 | 2023-07-27 | 阜阳师范大学 | Animal tissue culture processing apparatus |
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| WO2023138347A1 (en) | 2023-07-27 |
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