CN114477436A - Biological treatment method for denitrifying low-concentration nitrate nitrogen wastewater - Google Patents
Biological treatment method for denitrifying low-concentration nitrate nitrogen wastewater Download PDFInfo
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- 229910000357 manganese(II) sulfate Inorganic materials 0.000 claims description 4
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
- C02F3/2806—Anaerobic processes using solid supports for microorganisms
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/163—Nitrates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/006—Apparatus and plants for the biological treatment of water, waste water or sewage details of construction, e.g. specially adapted seals, modules, connections
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/15—N03-N
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
A biological treatment method for denitrifying low-concentration nitrate nitrogen wastewater. The present invention relates to the denitrification of NO in low-concentration nitrate nitrogen wastewater mainly by the genus Pseudoxanthomonas (Pseudomonas sp.) as a representative dominant bacterium3 ‑-N is removed, the steps in sequence being: preparing domesticated nutrient solution, filling an anaerobic bottle, culturing a biological membrane, pre-adjusting wastewater and biologically treating the wastewater. Can quickly and deeply remove NO in low-concentration nitrate nitrogen wastewater3 ‑and-N, the treatment period of the wastewater is shortened, the treatment cost is low, secondary pollution is avoided, and the method is simple and feasible, and is suitable for treating low-concentration nitrate nitrogen wastewater of various scales after amplification.
Description
Technical Field
The invention belongs to the technical field of sewage treatment, and particularly relates to a biological treatment method for denitrifying low-concentration nitrate nitrogen wastewater.
Background
Generally, the effluent of urban sewage treatment plants contains some nitrogen-containing compounds (such as nitrate nitrogen), and the discharge of the nitrogen-containing compounds into surface water and underground water causes eutrophication of water bodies and indirectly poses certain threats to human health safety. At present, in the first grade A standard of pollutant emission standards of municipal wastewater treatment plants in China, the Total Nitrogen (TN) limit value is 15 mg/L. With the establishment of more stringent standards, it is necessary to develop advanced denitrification techniques to treat nitrate nitrogen contaminants in secondary effluent.
Compared with other physical and chemical methods, the microbial method has certain superiority, can degrade nitrate nitrogen into gaseous nitrogen without generating other nitrogen-containing impurities, and has the characteristics of economy, high efficiency and environmental friendliness. However, further research into the treatment of low concentrations of nitrate nitrogen by microbiological methods is needed. Generally, biological denitrification can be classified into an activated sludge process and a biofilm process. Compared with free cells, the immobilized biofilm can show more stable and efficient operation effect. The immobilized microbe technology is one common biomembrane process, and can fix single or mixed microbe colony onto corresponding carrier and has the advantages of high biological density, high poison resistance, fast reaction system start, etc. Among them, the immobilization carrier plays a very important role. It not only has the function of supporting and providing sufficient space for the growth and proliferation of the microorganism, but also can form a protection mechanism for the microorganism, so that the microorganism is not easy to lose from the system. The nutrient substances can be adsorbed on the surface of the carrier and then penetrate through the matrix to be absorbed by the cells, so that the impact resistance of the microorganism is improved, the processes of film formation, starting and stabilization of a reaction system are accelerated, and the nitrate nitrogen sewage is efficiently treated. In addition, a dissolved oxygen concentration gradient is formed in the carrier, so that different flora can grow and develop. The functionalized polyurethane foam carrier is an organic polymer immobilized material with high strength and strong environment adaptability, and shows excellent performance in the aspect of microorganism immobilization.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide a practical and effective biological treatment process for low-concentration nitrate nitrogen wastewater.
The object of the present invention can be achieved by:
a biological treatment method for denitrifying low-concentration nitrate nitrogen wastewater is characterized by comprising the following steps: the method takes the functionalized polyurethane foam as a carrier and realizes the treatment of the low-concentration nitrate nitrogen wastewater through the denitrification of the immobilized microbial flora.
The biological treatment method comprises the following steps:
(1) preparing a domestication nutrient solution: the carbon nitrogen phosphorus element ratio of the domesticated nutrient solution is C: N: P is 100:10:1, and the pH value of the nutrient solution is regulated and controlled to be between 7.0 and 7.5;
(2) filling an anaerobic bottle: putting 25-35mg/L of the bacterial preparation and the pretreated functional polyurethane foam carrier into an anaerobic bottle filled with nutrient solution, and filling nitrogen to control the concentration of dissolved oxygen in the nutrient solution to be 0.5-1.0 mg/L;
(3) and (3) culturing a biological membrane: placing the anaerobic bottle in a constant-temperature incubator for culture; replacing part of nutrient solution after setting time at intervals, wherein the nutrient solution is obtained according to the step (1), and the concentration of dissolved oxygen is controlled according to the step (2); entering the step (4) when the biomembrane obviously grows on the carrier and the water quality is stable;
wherein the temperature in the constant temperature incubator is set to 25-28 ℃.
(4) Pre-conditioning the wastewater: for the low-concentration nitrate nitrogen wastewater, adding sodium acetate according to a preset carbon-nitrogen ratio, and simultaneously adjusting the pH value of the low-concentration nitrate nitrogen wastewater to 7.2 +/-0.1;
wherein the low-concentration nitrate nitrogen wastewater is initial NO3 -The N concentration is lower than 15mg/L, and the addition amount of the low-concentration nitrate nitrogen wastewater after pre-adjustment is 80-90% of the volume of the container.
(5) Biological treatment of wastewater: and (3) placing the regulated low-concentration nitrate nitrogen wastewater and the carrier acclimated to the biofilm into an anaerobic bottle at a set filling rate, filling nitrogen to maintain an anoxic environment, and then placing the anaerobic bottle into a constant-temperature incubator for culture, namely starting to carry out biological denitrification treatment on the wastewater.
The invention further comprises the following preferred embodiments:
the domesticated nutrient solution also comprises 20-22mg/L MgCl2·6H2O、0.5-1.5mg/L CaCl2And 0.5-1.5mL/L of trace elements.
The trace elements in the nutrient solution comprise: 0.02-0.04g/L NiCl2·6H2O、0.1-0.3g/L FeSO4·7H2O、0.02-0.04g/L CuSO4·5H2O、0.05-0.15g/L CoCl2·6H2O、0.05-0.15g/L MnSO4·H2O、0.02-0.06g/L Na2MoO4·2H2O、0.01-0.05g/L H3BO3。
In the step 1, the pH value of the nutrient solution is regulated and controlled by using 1mol/L hydrochloric acid and 1mol/L sodium hydroxide.
In step 2, the bacterial preparation is a complex microorganism comprising predominant genera such as Pseudomonas (Pseudomonas sp.), Zoogloea sp, Decloromonas sp, Cytophaga sp, Octophaga sp, Norrank _ f _ Cytophagaceae sp, and Norrank _ c _ OM 190. sp.
In step 2, before use, the functionalized polyurethane carrier is soaked in 5% hydrochloric acid and 5% sodium hydroxide solution for 24 hours respectively, washed to be neutral, and then is utilized after vacuum drying treatment at 60 ℃.
In step 3, replacing part of the nutrient solution at intervals of a set time means slowly pouring out 80% of the original solution after every 24 hours and supplementing the prepared new nutrient solution.
In step 4, the predetermined carbon-nitrogen ratio refers to COD and NO3 -The concentration ratio of-N to N is [5.0-5.8:1]。
In step 4, MgCl is further added2·6H2O、CaCl2And trace elements; wherein, MgCl2·6H2O is 20-22 mg/L; CaCl20.5-1.5 mg/L; the trace elements are 0.5-1.5 mL/L.
The trace elements in the nutrient solution are prepared as follows: 0.02-0.04g/L NiCl2·6H2O、0.1-0.3g/LFeSO4·7H2O、0.02-0.04g/L CuSO4·5H2O、0.05-0.15g/L CoCl2·6H2O、0.05-0.15g/LMnSO4·H2O、0.02-0.06g/L Na2MoO4·2H2O、0.01-0.05g/L H3BO3。
In step 4, the adjusted low-concentration nitrate nitrogen wastewater and the carrier acclimated to the biofilm are placed into an anaerobic bottle at a filling rate of 20-30%, nitrogen is filled to maintain the dissolved oxygen concentration at 0.5-1.0mg/L, then the anaerobic bottle is placed into a constant-temperature incubator for culture, namely, biological denitrification treatment is carried out on the wastewater; wherein the temperature of the constant temperature incubator is 25-28 ℃, and the rotating speed is set to 80-100 r/min per minute.
Compared with the prior art, the invention has the following advantages:
(1) the functionalized polyurethane material is used as a carrier in the immobilized denitrification technology, and based on the combination of the surface active chemical groups of the carrier and the functional groups of the biological membrane, the attachment of microorganisms can be promoted, and the impact resistance and the metabolic action of the microorganisms are improved. Meanwhile, the functionalized polyurethane material has a network structure with larger pore diameter, so that the blockage of the reactor can be effectively prevented, and the backwashing frequency is reduced.
(2) The diversity and the abundance of floras in the interior and the surface area of a functional polyurethane foam carrier in an immobilized microorganism system have certain difference, and the denitrification process mainly occurs in the interior space of the carrier taking Pseudoxanthomonas (Pseudoxanthomonas sp) as dominant bacteria, so that the impact resistance of the microorganism can be improved, and the stable degradation of low-concentration nitrate nitrogen wastewater by the microorganism is facilitated.
(3) The method can lead the low-concentration nitrate nitrogen wastewater (initial NO)3 -NO in N concentrations lower than 15mg/L)3 -N is reduced to below 0.1mg/L within 5h and is almost completely removed, and finally, no nitrite nitrogen is accumulated, so that the harm to organisms and human bodies after water body discharge can be reduced.
(3) The biological treatment method can greatly reduce the wastewater treatment cost, avoid the problem of secondary pollution caused by physical and chemical treatment, and achieve better advanced treatment effect.
(4) The restoration process is simple and easy to implement, and low-concentration nitrate nitrogen wastewater of various scales can be treated through amplification to a certain degree.
Drawings
FIG. 1 is a flow chart of the present invention for biologically treating low concentration nitrate nitrogen wastewater.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. The embodiments described herein are only a part of the embodiments of the present invention, and not all of them. All other embodiments obtained by a person skilled in the art without making any inventive step on the basis of the spirit of the present invention are within the scope of protection of the present invention.
As shown in figure 1, the invention discloses an immobilized microorganism process flow for deep denitrification treatment of low-concentration nitrate nitrogen, which specifically comprises the following steps:
(1) preparing a domestication nutrient solution:
the carbon nitrogen phosphorus element ratio of the domesticated nutrient solution is C: N: P is 100:10:1, and the pH of the nutrient solution is regulated and controlled to be [7.0,7.5]]In the meantime. The domesticated nutrient solution also comprises 20-22mg/L MgCl2·6H2O、0.5-1.5mg/L CaCl2And 0.5-1.5mg/L of trace elements.
In a preferred embodiment of the invention, an acclimatized nutrient solution with a nitrate concentration of 20mg/L is prepared, wherein the acclimatized nutrient solution contains 144.28mg/L potassium nitrate, 256.25mg/L sodium acetate, 8.77mg/L monopotassium phosphate and 20.3mg/L MgCl2·6H2O、1mg/L CaCl2And 1mL/L of trace elements.
The trace elements in the nutrient solution are prepared as follows: 0.03g/L NiCl2·6H2O、0.2g/L FeSO4·7H2O、0.03g/L CuSO4·5H2O、0.1g/L CoCl2·6H2O、0.1g/L MnSO4·H2O、0.04g/LNa2MoO4·2H2O、0.03g/L H3BO3. Regulating pH of the nutrient solution to 7.0-7.5 with 1mol/L hydrochloric acid and 1mol/L sodium hydroxide.
(2) Filling an anaerobic bottle:
and (3) putting 25-35mg/L of the bacterial preparation and the pretreated functional polyurethane foam carrier into an anaerobic bottle filled with nutrient solution, and controlling the concentration of dissolved oxygen in the nutrient solution filled with nitrogen to be 0.5-1.0 mg/L.
The bacterial preparation is a compound microorganism containing predominant bacteria such as Pseudomonas (Pseudoxanthomonas sp.), Zoogloea sp., Dechloromonas sp., Haliscomenobacter sp., Achnotus sp., Caulobacter sp., norak _ f _ Cytophagae sp., norak _ c _ OM190 sp.
In a preferred embodiment of the present invention, the bacterial preparation is a highly effective microbial complex preparation which is purchased from environmental technologies, Inc., of Togaku, Beijing under the name FZ-B-2. 30mg/L of the bacterial preparation and the pretreated functional polyurethane foam carrier are put into an anaerobic bottle filled with 500mL of nutrient solution, and nitrogen is filled to control the concentration of dissolved oxygen in the nutrient solution to be 0.5-1.0 mg/L.
Further preferably, the functionalized polyurethane carrier is soaked in 5% hydrochloric acid and 5% sodium hydroxide solution for 24 hours respectively before use, washed to be neutral, and subjected to vacuum drying treatment at 60 ℃ for reuse.
(3) And (3) culturing a biological membrane:
placing the anaerobic bottle in a constant-temperature incubator for culture; replacing part of nutrient solution after setting time at intervals, obtaining the nutrient solution according to the step (1), and controlling the concentration of dissolved oxygen according to the step (2); entering the step (4) when the biomembrane obviously grows on the carrier and the water quality is stable;
the anaerobic flask was placed in a 25 ℃ incubator at 90 rpm for culture. After every 24 hours, 80% of the stock solution is slowly poured out and the prepared new nutrient solution is supplemented. When the carrier obviously grows with a biological membrane and the water quality is stable, the next stage of experimental study can be carried out. A completely effective immobilized microorganism system can be established within 8 days, and the denitrification efficiency can reach more than 99 percent every 24 hours.
(4) Pre-conditioning the wastewater:
for low concentration nitrate nitrogen wastewater (initial NO)3 -N concentration lower than 15mg/L), according to the carbon to nitrogen ratio (COD and NO)3 --N concentration ratio) of 5.0-5.8:1, adding a certain amount of sodium acetate and simultaneously adding 20.3mg/L of MgCl2·6H2O、1mg/L CaCl2And 1mL/L of a trace element solution using 1mol/L hydrochloric acidAnd 1mol/L sodium hydroxide regulates the pH value of the wastewater to be 7.2 +/-0.1.
(5) Biological treatment of wastewater:
the adjusted low-concentration nitrate nitrogen wastewater and the carrier of the domesticated biofilm are placed in a 500mL anaerobic bottle at a filling rate of 25%, nitrogen is filled in the anaerobic bottle to maintain an anoxic environment (the dissolved oxygen concentration is 0.5-1.0mg/L), and then the anaerobic bottle is placed in a constant-temperature incubator at 25 ℃ for culture, and the rotating speed is set to 90 revolutions per minute.
In order to make the reader better understand the technical scheme of the invention, 1 example is listed below to further explain the invention, but it should be clear to those skilled in the art that the invention is not limited to this example only.
The specific implementation mode of treating low-pollution water in a river by adopting the method is as follows:
(1) preparing acclimatized nutrient solution with nitrate concentration of 20mg/L, wherein the acclimatized nutrient solution contains 144.28mg/L potassium nitrate, 256.25mg/L sodium acetate, 8.77mg/L monopotassium phosphate and 20.3mg/L MgCl2·6H2O、1mg/L CaCl2And 1mL/L of trace elements. Regulating pH of the nutrient solution to 7.0-7.5 with 1mol/L hydrochloric acid and 1mol/L sodium hydroxide.
(2) 30mg/L of the bacterial preparation FZ-B-2 and the pretreated functional polyurethane foam carrier are put into an anaerobic bottle filled with 500mL of nutrient solution, and nitrogen is filled to control the concentration of dissolved oxygen in the nutrient solution to be 0.5-1.0 mg/L.
(3) Placing the anaerobic bottle in a constant-temperature incubator at 25 ℃ for culture, setting the rotating speed at 90 r/min, slowly pouring out 80% stock solution after every 24 hours, and supplementing the prepared new nutrient solution. When the carrier obviously grows with a biological membrane and the water quality is stable, the next stage of experimental study can be carried out. A completely effective immobilized microorganism system can be established within 8 days, and the denitrification efficiency can reach more than 99 percent every 24 hours.
(4) For low concentration nitrate nitrogen wastewater (initial NO)3 -N concentration lower than 15mg/L), according to the carbon to nitrogen ratio (COD and NO)3 -Concentration ratio of-N) of 5.4:1, a certain amount of sodium acetate was added together with 20.3mg/L of MgCl2·6H2O、1mg/L CaCl2And 1mL/L of trace element solution, and regulating the pH value of the wastewater to be 7.2 +/-0.1 by utilizing 1mol/L of hydrochloric acid and sodium hydroxide.
(5) The adjusted low-concentration nitrate nitrogen wastewater and the carrier of the domesticated biofilm are placed into a 500mL anaerobic bottle at a filling rate of 25%, nitrogen is filled in the anaerobic bottle to maintain an anoxic environment (the dissolved oxygen concentration is 0.5-1.0mg/L), and then the anaerobic bottle is placed into a constant-temperature incubator at 25 ℃ for culture, and the rotating speed is set to 90 revolutions per minute.
(6) Low concentration nitrate nitrogen wastewater (initial NO)3 -N concentration lower than 15mg/L) can be almost completely removed within 5h, NO3 --N concentration less than 0.1mg/L and finally no nitrite nitrogen accumulation.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting the same, and although the present invention is described in detail with reference to the above embodiments, those of ordinary skill in the art should understand that: modifications and equivalents may be made to the embodiments of the invention without departing from the spirit and scope of the invention, which is to be covered by the claims.
Claims (11)
1. A biological treatment method for denitrifying low-concentration nitrate nitrogen wastewater is characterized by comprising the following steps: the method comprises the following steps of (1) treating low-concentration nitrate nitrogen wastewater by using functionalized polyurethane foam as a carrier and through the denitrification of immobilized flora; wherein the low-concentration nitrate nitrogen wastewater is initial NO3 --N concentration lower than 15 mg/L.
2. The biological treatment method for the denitrification treatment of low-concentration nitrate nitrogen wastewater according to claim 1, characterized in that the biological treatment method comprises the steps of:
(1) preparing a domestication nutrient solution: the carbon nitrogen phosphorus element ratio of the domesticated nutrient solution is C: N: P is 100:10:1, and the pH value of the nutrient solution is regulated and controlled to be between 7.0 and 7.5;
(2) filling an anaerobic bottle: putting 25-35mg/L of the bacterial preparation and the pretreated functional polyurethane foam carrier into an anaerobic bottle filled with nutrient solution, and filling nitrogen to control the concentration of dissolved oxygen in the nutrient solution to be 0.5-1.0 mg/L;
(3) and (3) culturing a biological membrane: placing the anaerobic bottle in a constant-temperature incubator for culture; replacing part of nutrient solution after setting time at intervals, wherein the nutrient solution is obtained according to the step (1), and the concentration of dissolved oxygen is controlled according to the step (2); entering the step (4) when the biomembrane obviously grows on the carrier and the water quality is stable;
wherein the temperature in the constant temperature incubator is set to 25-28 ℃.
(4) Pre-conditioning the wastewater: for the low-concentration nitrate nitrogen wastewater, adding sodium acetate according to a preset carbon-nitrogen ratio, and simultaneously adjusting the pH value of the low-concentration nitrate nitrogen wastewater to 7.2 +/-0.1;
wherein the addition amount of the low-concentration nitrate nitrogen wastewater after the pre-adjustment is 80-90% of the volume of the container;
(5) biological treatment of wastewater: and (3) placing the regulated low-concentration nitrate nitrogen wastewater and the carrier acclimated to the biofilm into an anaerobic bottle at a set filling rate, filling nitrogen to maintain an anoxic environment, and then placing the anaerobic bottle into a constant-temperature incubator for culture, namely starting to carry out biological denitrification treatment on the wastewater.
3. The biological treatment method for the denitrification treatment of nitrate nitrogen wastewater according to claim 2, characterized in that:
the domesticated nutrient solution also comprises 20-22mg/L MgCl2·6H2O、0.5-1.5mg/L CaCl2And 0.5-1.5mL/L of trace elements.
4. The biological treatment method for the denitrification treatment of nitrate nitrogen wastewater according to claim 3, characterized in that:
the trace elements in the nutrient solution comprise: 0.02-0.04g/L NiCl2·6H2O、0.1-0.3g/L FeSO4·7H2O、0.02-0.04g/L CuSO4·5H2O、0.05-0.15g/L CoCl2·6H2O、0.05-0.15g/L MnSO4·H2O、0.02-0.06g/L Na2MoO4·2H2O、0.01-0.05g/L H3BO3。
5. The biological treatment method for the denitrification treatment of low-concentration nitrate nitrogen wastewater according to claim 2, characterized in that:
in the step 1, the pH value of the nutrient solution is regulated and controlled by using 0.5-1mol/L hydrochloric acid and 0.5-1mol/L sodium hydroxide.
6. The biological treatment method for the denitrification treatment of nitrate nitrogen wastewater according to claim 2, characterized in that:
in step 2, the bacterial preparation is a complex microorganism comprising predominant genera such as Pseudomonas (Pseudomonas sp.), Zoogloea sp, Decloromonas sp, Cytophaga sp, Octophaga sp, Norrank _ f _ Cytophagaceae sp, and Norrank _ c _ OM 190. sp.
7. The biological treatment method for the denitrification treatment of low-concentration nitrate nitrogen wastewater according to claim 2, characterized in that:
in step 2, before use, the functionalized polyurethane carrier is soaked in 5% hydrochloric acid and 5% sodium hydroxide solution for 24 hours respectively, washed to be neutral, and then is utilized after vacuum drying treatment at 60 ℃.
8. The biological treatment method for the denitrification treatment of low-concentration nitrate nitrogen wastewater according to claim 2, characterized in that:
in step 3, replacing part of the nutrient solution at intervals of a set time means slowly pouring out 80% of the original solution after every 24 hours and supplementing the prepared new nutrient solution.
9. The biological treatment method for the denitrification treatment of nitrate nitrogen wastewater according to claim 2, characterized in that:
in step 4, the predetermined carbon-nitrogen ratio refers to COD and NO3 -The concentration ratio of-N to N is [5.0-5.8:1]。
10. The biological treatment method for the denitrification treatment of low-concentration nitrate nitrogen wastewater according to claim 9, characterized in that:
in step 4, MgCl is further added2·6H2O、CaCl2And trace elements; wherein, MgCl2·6H2O is 20-22 mg/L; CaCl20.5-1.5 mg/L; the trace elements are 0.5-1.5 mL/L.
The trace elements in the nutrient solution are prepared as follows: 0.02-0.04g/L NiCl2·6H2O、0.1-0.3g/L FeSO4·7H2O、0.02-0.04g/L CuSO4·5H2O、0.05-0.15g/L CoCl2·6H2O、0.05-0.15g/L MnSO4·H2O、0.02-0.06g/L Na2MoO4·2H2O、0.01-0.05g/L H3BO3。
11. The biological treatment method for the denitrification treatment of low-concentration nitrate nitrogen wastewater according to claim 1 or 10, characterized in that:
in step 4, the adjusted low-concentration nitrate nitrogen wastewater and the carrier acclimated to the biofilm are placed into an anaerobic bottle at a filling rate of 20-30%, nitrogen is filled to maintain the dissolved oxygen concentration at 0.5-1.0mg/L, then the anaerobic bottle is placed into a constant-temperature incubator for culture, namely, biological denitrification treatment is carried out on the wastewater; wherein the temperature of the constant temperature incubator is 25-28 ℃, and the rotating speed is set to 80-100 r/min.
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