CN114476330B - Soil storage and freeze-drying sample bottle for microorganism detection and detection method thereof - Google Patents
Soil storage and freeze-drying sample bottle for microorganism detection and detection method thereof Download PDFInfo
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- CN114476330B CN114476330B CN202210186032.XA CN202210186032A CN114476330B CN 114476330 B CN114476330 B CN 114476330B CN 202210186032 A CN202210186032 A CN 202210186032A CN 114476330 B CN114476330 B CN 114476330B
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- sample
- inner panel
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- 239000002689 soil Substances 0.000 title claims abstract description 38
- 238000004108 freeze drying Methods 0.000 title claims abstract description 29
- 238000001514 detection method Methods 0.000 title claims abstract description 22
- 244000005700 microbiome Species 0.000 title claims abstract description 16
- 238000005192 partition Methods 0.000 claims abstract description 23
- 239000003381 stabilizer Substances 0.000 claims description 9
- 239000011159 matrix material Substances 0.000 claims description 6
- 230000000087 stabilizing effect Effects 0.000 claims description 5
- 238000007710 freezing Methods 0.000 claims description 3
- 230000008014 freezing Effects 0.000 claims description 3
- 230000002906 microbiologic effect Effects 0.000 claims 6
- 238000010998 test method Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 6
- 239000000843 powder Substances 0.000 abstract description 6
- -1 Phospholipid fatty acid Chemical class 0.000 description 7
- 238000007789 sealing Methods 0.000 description 7
- 239000000463 material Substances 0.000 description 5
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 5
- 239000004810 polytetrafluoroethylene Substances 0.000 description 5
- 239000005388 borosilicate glass Substances 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/02—Internal fittings
- B65D25/04—Partitions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D21/00—Nestable, stackable or joinable containers; Containers of variable capacity
- B65D21/02—Containers specially shaped, or provided with fittings or attachments, to facilitate nesting, stacking, or joining together
- B65D21/0209—Containers specially shaped, or provided with fittings or attachments, to facilitate nesting, stacking, or joining together stackable or joined together one-upon-the-other in the upright or upside-down position
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D23/00—Details of bottles or jars not otherwise provided for
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D51/00—Closures not otherwise provided for
- B65D51/16—Closures not otherwise provided for with means for venting air or gas
- B65D51/1672—Closures not otherwise provided for with means for venting air or gas whereby venting occurs by manual actuation of the closure or other element
- B65D51/1683—Closures not otherwise provided for with means for venting air or gas whereby venting occurs by manual actuation of the closure or other element by actuating a separate element in the container or closure
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
Landscapes
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
A soil storage and freeze-drying sample bottle for microorganism detection and a detection method thereof relate to the technical field of soil sample pretreatment. The invention aims to solve the problems that soil powder in the existing sample freeze-drying process is easy to be pumped by a vacuum pump, so that the sample is lost, the cavity of a vacuum dryer is polluted, and even the vacuum pump is damaged. The invention comprises a sample storage bottle, a partition plate, a bottle cap knob and two bottle cap inner panels, wherein the partition plate is arranged in the sample storage bottle, the bottle cap is sleeved on the outer side of the bottle mouth of the sample storage bottle, a group of bottle cap through holes are formed in the upper end face of the bottle cap, the middle part of the upper end face of the bottle cap is rotationally connected with the bottle cap knob, a cover plate is fixedly connected onto the bottle cap knob, the cover plate is arranged on the outer side of the bottle cap through holes, the two bottle cap inner panels are parallelly embedded on the inner side of the bottle cap, a group of inner panel through holes are formed in one side of the bottle cap inner panels, and the inner panel through holes on the two bottle cap inner panels are arranged in a staggered manner. The invention is used for detecting soil microorganisms.
Description
Technical Field
The invention relates to the technical field of soil sample pretreatment, in particular to a soil storage and freeze-drying sample bottle for microorganism detection and a detection method thereof.
Background
Phospholipid fatty acid analysis (Phospholipid fatty acid, PLFA) is an important method for qualitative and quantitative analysis of soil microorganisms, and soil samples for PLFA analysis need to be stored at low temperature after collection and freeze-dried in a freeze dryer, and conventional soil samples are often placed in self-sealing bags after collection, returned to a laboratory, and then the self-sealing bags are opened and placed in a freeze dryer for freeze-drying. However, soil powder is easily pumped out by a vacuum freeze dryer in the freeze drying process, so that the cavity of the freeze dryer is polluted and the sample is lost, and even the powder is pumped into a vacuum pump, so that the vacuum pump is damaged. In addition, in order to avoid introducing organic pollutants, the sample should be prevented from contacting plastic products except polytetrafluoroethylene in soil microorganism detection, so that a plastic self-sealing bag should be avoided as much as possible.
Disclosure of Invention
The invention aims to rapidly and efficiently finish soil sample storage and freeze-drying work, so as to solve the problems that soil powder in the actual sample freeze-drying process, which is proposed in the background art, is easily pumped by a vacuum pump, so that the sample is lost, the cavity of a vacuum dryer is polluted, and even the vacuum pump is damaged, and further provides a soil storage and freeze-drying sample bottle for microorganism detection and a detection method thereof.
The technical scheme adopted for solving the technical problems is as follows:
a sample bottle for microorganism detection's soil is stored and freeze-dried includes sample storage bottle, the division board, the bottle lid, bottle lid knob and two bottle lid inner panels, the upper end bottleneck of sample storage bottle is uncovered to be set up, the division board sets up in the sample storage bottle and cuts apart the sample storage bottle into a plurality of storage areas of equidimension, the bottle lid suit is in the outside of sample storage bottle bottleneck, be equipped with a set of bottle lid through-hole on the up end of bottle lid, the middle part rotation of bottle lid up end is connected with the bottle lid knob, the rigid coupling has the apron on the bottle lid knob, the apron sets up the outside at the bottle lid through-hole, two bottle lid inner panels are inlayed in the inboard parallel of bottle lid, one side on the inner panel of bottle lid is equipped with a set of inner panel through-hole, the inner panel through-hole on two bottle lid inner panels is crisscross to be set up.
Further, the shape of the sample storage bottle is cylindrical, and the bottleneck of the sample storage bottle extends outwards in an arc-shaped curved surface.
Further, the bottle cap is cylindrical in shape, the lower end of the bottle cap is opened, two annular grooves are formed in the inner circumferential side wall of the bottle cap in parallel, and the outer side edge of the inner panel of the bottle cap is arranged in the annular grooves.
Further, the bottle cap knob comprises a rotating shaft and two poking sheets, the rotating shaft is vertically fixedly connected to the middle of the upper end face of the bottle cap, the two poking sheets are vertically arranged in a crisscross shape, the middle of each poking sheet is sleeved on the rotating shaft, and the cover plate is fixedly connected to the lower portion between the two poking sheets.
Further, the cover plate is in a quarter fan shape, one side of the cover plate is fixedly connected with the lower end of one side of one plectrum, and the other side of the cover plate is fixedly connected with the lower end of one side of the other plectrum.
Further, the group of bottle cap through holes comprises a plurality of bottle cap through holes which are uniformly distributed in a matrix shape, and the plurality of bottle cap through holes are arranged in a quarter sector area on the bottle cap by taking the rotating shaft as the center of a circle.
Further, the group of inner panel through holes comprises a plurality of inner panel through holes which are uniformly distributed in a matrix shape, and the plurality of inner panel through holes are arranged in a square area at one side of the inner panel.
Further, the soil storage and freeze-drying sample bottle for microorganism detection further comprises a stacking stabilizer, and the stacking stabilizer is fixedly connected to the outer side edge of the upper end face of the bottle cap.
Further, the stacking stabilizing frame comprises a plurality of limiting arc plates, the limiting arc plates are uniformly distributed along the circumferential direction of the bottle cap, and the lower ends of the limiting arc plates are fixedly connected to the outer side edges of the upper end face of the bottle cap.
The detection method of the soil storage and freeze-dried sample bottle for microorganism detection comprises the following steps:
step one: firstly, placing a partition plate in a sample storage bottle, and uniformly distributing collected soil samples into a plurality of areas separated by the partition plate in the sample storage bottle;
step two: the two inner panels of the bottle cap are arranged in the bottle cap, the inner panel through holes on the inner panel of the upper layer of the bottle cap and the inner panel through holes on the bottle cap are arranged in a 180-degree staggered mode, and the inner panel through holes on the inner panel of the lower layer of the bottle cap and the inner panel through holes on the inner panel of the upper layer of the bottle cap are arranged in a 180-degree staggered mode;
step three: rotating the bottle cap knob, rotating the cover plate to the outer side of the bottle cap through hole to enable the bottle cap through hole to be in a closed state, and then covering the bottle cap on the bottle mouth of the sample storage bottle;
step four: firstly, placing a sample storage bottle into a refrigerator at the temperature of minus 20 ℃ for pre-freezing, then placing the sample storage bottle into a freeze dryer, rotating a bottle cap knob, keeping a cover plate away from a bottle cap through hole, enabling the bottle cap through hole to be in an open state, stacking a plurality of sample storage bottles along the height direction under the clamping of a stacking stabilizing frame, and after the sample storage bottles are placed, starting the freeze dryer for freeze drying.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a soil storage and freeze-drying sample bottle for microorganism detection, which avoids sample pollution possibly introduced by contacting a plastic self-sealing bag with a sample, and simultaneously adopts 180-degree cross-opened air holes to reduce the probability of extracting soil powder by a vacuum pump, solve the problem that the existing soil sample is easily extracted by the vacuum freeze dryer to pollute the cavity of the freeze dryer in the freeze-drying process, and avoid the sample from polluting the cavity of the vacuum dryer and damaging the vacuum pump.
The sample bottle is provided with the partition plate, and the sample storage bottle is divided into a plurality of areas under the isolation of the partition plate, so that the bulk volume of soil samples is reduced, and the freeze drying rate is improved.
Meanwhile, the sample bottles can be stacked, so that the cavity space of the vacuum freeze dryer can be fully utilized, the number of freeze-dried samples in the same batch can be increased, and the freeze-drying efficiency can be improved.
Compared with the conventional self-sealing bag freeze-drying, the soil powder pumped by the vacuum pump is reduced by more than 80 percent.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of the structure of a sample storage bottle 1 according to the present invention;
FIG. 3 is a schematic view showing the structure of the bottle cap 3, the bottle cap knob 5 and the stacked stabilizer 6 according to the present invention;
fig. 4 is a schematic view of the structure of the partition plate 2 in the present invention;
fig. 5 is a schematic view showing the structure of the cover plate 51 outside the cap through hole 31 in the present invention;
fig. 6 is a schematic view of the structure of the cover plate 51 of the present invention away from the outer side of the cap through hole 31;
fig. 7 is a schematic view of the structure of the inner panel 4 of the bottle cap according to the present invention.
Detailed Description
The first embodiment is as follows: referring to fig. 1-7, in this embodiment, a soil storage and freeze-drying sample bottle for detecting microorganisms includes a sample storage bottle 1, a partition plate 2, a bottle cap 3, a bottle cap knob 5 and two bottle cap inner panels 4, wherein the upper end bottle mouth of the sample storage bottle 1 is opened, the partition plate 2 is arranged in the sample storage bottle 1 and divides the sample storage bottle 1 into a plurality of storage areas with the same size, the bottle cap 3 is sleeved on the outer side of the bottle mouth of the sample storage bottle 1, a group of bottle cap through holes 31 are arranged on the upper end surface of the bottle cap 3, the bottle cap knob 5 is rotationally connected with the middle part of the upper end surface of the bottle cap 3, a cover plate 51 is fixedly connected with the bottle cap knob 5, the cover plate 51 is arranged on the outer side of the bottle cap through holes 31, two bottle cap inner panels 4 are parallelly embedded on the inner side of the bottle cap inner panels 4, and the inner through holes 41 on the two bottle cap inner panels 4 are staggered.
The inner panel through holes 41 on the upper inner panel 4 of the bottle cap are staggered with the bottle cap through holes 31 on the bottle cap 3 by 180 degrees, and the inner panel through holes 41 on the lower inner panel 4 of the bottle cap are staggered with the inner panel through holes 41 on the upper inner panel 4 of the bottle cap by 180 degrees.
The middle part of the lower end surface of the inner panel 4 of the bottle cap is fixedly connected with a handle 42 for installing and detaching the inner panel 4 of the bottle cap.
The inner panel 4 of the bottle cap is made of polytetrafluoroethylene material, is round in shape, and has a diameter of 8cm and a thickness of 0.2cm.
The partition plate 2 is a movable member and can be directly placed in the sample storage bottle 1.
The shape of the partition plate 2 is in a shape of a Chinese character 'mi', the partition plate 2 comprises three partition plates, the partition plates are vertically arranged, and the middle parts of the three partition plates are coaxially intersected. The separation plate 2 is made of three pieces of high borosilicate glass which are coaxially intersected, and divides the sample storage bottle 1 into six areas with the same size. Each piece of borosilicate glass of the partition plate 2 has a height of 8cm and a length of 7cm. Under the isolation of the partition plate 2, the sample storage bottle 1 is partitioned into 6 areas to reduce the bulk volume of the soil sample and improve the freeze drying rate.
The second embodiment is as follows: referring to fig. 1 to 7, the shape of the sample storage bottle 1 according to the present embodiment is cylindrical, and the mouth of the sample storage bottle 1 extends outwards in an arc-shaped curved surface. The technical features not disclosed in this embodiment are the same as those of the first embodiment.
The sample storage bottle 1 is made of brown high borosilicate glass material, has a size of 10cm, an outer diameter of 8cm, an inner diameter of 7.5cm, and an arc-shaped outer roll of 0.5cm is arranged on the top bottle opening.
The body of the sample storage bottle 1 is provided with scale marks 11 along the height direction.
The body of the sample storage bottle 1 is provided with a sample information recording label 12.
And a third specific embodiment: referring to fig. 1 to 7, in the present embodiment, the bottle cap 3 is cylindrical, the lower end of the bottle cap 3 is opened, two ring grooves 32 are parallel arranged on the inner circumferential side wall of the bottle cap 3, and the outer edge of the inner panel 4 of the bottle cap is arranged in the ring groove 32. The technical features not disclosed in this embodiment are the same as those of the second embodiment.
The bottle cap 3 is made of polytetrafluoroethylene materials, the size is 4cm, the inner diameter is 8cm, and the outer diameter is 9cm.
And a sealing rubber strip is arranged at one side of the interface between the bottom of the bottle cap 3 and the sample storage bottle 1.
The two annular grooves 32 are spaced by 1cm, and sealing gaskets are arranged in the annular grooves 32 and used for fixing the inner panel 4 of the bottle cap.
The specific embodiment IV is as follows: referring to fig. 1 to 7, in this embodiment, the knob 5 for bottle cap includes a rotating shaft 52 and two paddles 53, the rotating shaft 52 is vertically fixed on the middle of the upper end surface of the bottle cap 3, the paddles 53 are vertically arranged in a crisscross shape, the middle of the paddles 53 is sleeved on the rotating shaft 52, and the cover plate 51 is fixed on the lower part between the paddles 53. The technical features not disclosed in this embodiment are the same as those of the first, second or third embodiments.
The bottle cap knob 5 is made of polytetrafluoroethylene materials, the center of the knob is a rotating shaft 52 with the diameter of 0.5cm and the height of 1cm, and the rotating shaft is poured in the center of the bottle cap 3.
The length of the pulling piece 53 is 6cm, and the height is 1cm.
Fifth embodiment: referring to fig. 1 to 7, the cover plate 51 of the present embodiment has a quarter fan shape, one side of the cover plate 51 is fixedly connected to the lower end of one side of one of the paddles 53, and the other side of the cover plate 51 is fixedly connected to the lower end of one side of the other paddle 53. The technical features not disclosed in this embodiment are the same as those of the fourth embodiment.
A sector cover plate 51 is arranged in a quarter area between the poking pieces 53, and the diameter of the cover plate 51 is 3cm, and the thickness of the cover plate is 0.2cm.
Specific embodiment six: referring to fig. 1 to 7, in the present embodiment, the group of cap through holes 31 includes a plurality of cap through holes 31 uniformly distributed in a matrix shape, and the plurality of cap through holes 31 are disposed in a quarter sector area around the rotation shaft 52 on the cap 3. The technical features not disclosed in this embodiment are the same as those of the fifth embodiment.
The diameter of the sector area is 2cm, and the aperture of the bottle cap through hole 31 is 0.15mm.
Seventh embodiment: the present embodiment will be described with reference to fig. 1 to 7, in which the set of inner panel through holes 41 includes a plurality of inner panel through holes 41 uniformly distributed in a matrix, and the plurality of inner panel through holes 41 are provided in a square region on one side of the inner panel 4. The technical features not disclosed in this embodiment are the same as those of the sixth embodiment.
The square area has a size of 1.5cm×1.5cm, and the hole diameter of the inner panel through hole 41 is 0.15mm.
Eighth embodiment: referring to fig. 1 to 7, the soil storage and freeze-drying sample bottle for detecting microorganisms according to the present embodiment further includes a stacking stabilizer 6, where the stacking stabilizer 6 is fixedly connected to an outer edge of an upper end surface of the bottle cap 3. The technical features not disclosed in this embodiment are the same as those of the first, second, third, fifth, sixth or seventh embodiment.
The stacking stabilizer 6 is made of polytetrafluoroethylene materials, is poured around the bottle cap 3, is 3cm in height, and is in an arc shape to slightly clamp the sample storage bottle 1.
Detailed description nine: referring to fig. 1 to 7, in this embodiment, the stacking stabilizer 6 includes a plurality of limiting arc plates, where the plurality of limiting arc plates are uniformly distributed along the circumferential direction of the bottle cap 3, and the lower ends of the limiting arc plates are fixedly connected to the outer edges of the upper end surfaces of the bottle cap 3. The technical features not disclosed in this embodiment are the same as those of the eighth embodiment.
Detailed description ten: referring to fig. 1 to 7, the method for detecting a soil storage and freeze-drying sample bottle for detecting microorganisms according to the present embodiment includes the following steps:
step one: firstly, placing a partition plate 2 in a sample storage bottle 1, and uniformly distributing collected soil samples into a plurality of areas separated by the partition plate 2 in the sample storage bottle 1;
step two: two inner bottle cap panels 4 are installed in the bottle cap 3, the inner panel through holes 41 on the inner bottle cap panel 4 of the upper layer are staggered with the bottle cap through holes 31 on the bottle cap 3 at 180 degrees, and the inner panel through holes 41 on the inner bottle cap panel 4 of the lower layer are staggered with the inner panel through holes 41 on the inner bottle cap panel 4 of the upper layer at 180 degrees;
step three: rotating the bottle cap knob 5, rotating the cover plate 51 to the outer side of the bottle cap through hole 31 to enable the bottle cap through hole 31 to be in a closed state, and then covering the bottle cap 3 on the bottle mouth of the sample storage bottle 1;
step four: firstly, placing the sample storage bottles 1 into a refrigerator with the temperature of minus 20 ℃ for pre-freezing, then placing the sample storage bottles 1 into a freeze dryer, rotating a bottle cap knob 5, keeping a cover plate 51 away from a bottle cap through hole 31, enabling the bottle cap through hole 31 to be in an open state, stacking a plurality of sample storage bottles 1 along the height direction under the clamping of a stacking stabilizing frame 6, and after the sample storage bottles 1 are placed, starting the freeze dryer for freeze drying.
Although the invention herein has been described with reference to particular embodiments, it is to be understood that these embodiments are merely illustrative of the principles and applications of the present invention. It is therefore to be understood that numerous modifications may be made to the illustrative embodiments and that other arrangements may be devised without departing from the spirit and scope of the present invention as defined by the appended claims. It should be understood that the different dependent claims and the features described herein may be combined in ways other than as described in the original claims. It is also to be understood that features described in connection with separate embodiments may be used in other described embodiments.
Claims (6)
1. Soil storage and lyophilization sample bottles for microbiological detection characterized in that: the novel bottle cap comprises a sample storage bottle (1), a partition plate (2), a bottle cap (3), a bottle cap knob (5) and two bottle cap inner panels (4), wherein the bottle mouth at the upper end of the sample storage bottle (1) is opened, the partition plate (2) is arranged in the sample storage bottle (1) and divides the sample storage bottle (1) into a plurality of storage areas with the same size, the bottle cap (3) is sleeved on the outer side of the bottle mouth of the sample storage bottle (1), a group of bottle cap through holes (31) are formed in the upper end face of the bottle cap (3), the bottle cap knob (5) is rotationally connected with the middle part of the upper end face of the bottle cap (3), a cover plate (51) is fixedly connected with the bottle cap knob (5), the cover plate (51) is arranged on the outer side of the bottle cap through holes (31), two bottle cap inner panels (4) are parallelly embedded in the inner side of the bottle cap (3), one side of the bottle cap inner panels (4) is provided with a group of inner panel through holes (41), and the inner through holes (41) on the two bottle cap inner panels (4) are staggered.
The inner panel through holes (41) on the inner panel (4) of the upper layer of bottle cap are staggered with the bottle cap through holes (31) on the bottle cap (3) at 180 degrees, and the inner panel through holes (41) on the inner panel (4) of the lower layer of bottle cap are staggered with the inner panel through holes (41) on the inner panel (4) of the upper layer of bottle cap at 180 degrees;
the bottle cap (3) is cylindrical, the lower end of the bottle cap (3) is opened, two annular grooves (32) are formed in parallel on the inner circumferential side wall of the bottle cap (3), and the outer side edge of the inner bottle cap panel (4) is arranged in the annular grooves (32);
the bottle cap knob (5) comprises a rotating shaft (52) and two poking sheets (53), the rotating shaft (52) is vertically fixedly connected to the middle of the upper end face of the bottle cap (3), the two poking sheets (53) are vertically arranged in a crisscross shape, the middle of each poking sheet (53) is sleeved on the rotating shaft (52), a cover plate (51) is fixedly connected to the lower portion between the two poking sheets (53), the cover plate (51) is in a quarter fan shape, one side of the cover plate (51) is fixedly connected with the lower end of one side of one poking sheet (53), and the other side of the cover plate (51) is fixedly connected with the lower end of one side of the other poking sheet (53);
the group of bottle cap through holes (31) comprises a plurality of bottle cap through holes (31) which are uniformly distributed in a matrix shape, and the plurality of bottle cap through holes (31) are arranged in a quarter sector area on the bottle cap (3) by taking the rotating shaft (52) as the center of a circle.
2. The soil storage and lyophilization sample bottle for microbiological detection of claim 1 wherein: the sample storage bottle (1) is cylindrical in shape, and the bottle mouth of the sample storage bottle (1) extends outwards in an arc-shaped curved surface.
3. The soil storage and lyophilization sample bottle for microbiological detection of claim 1 wherein: the group of inner panel through holes (41) comprises a plurality of inner panel through holes (41) which are uniformly distributed in a matrix shape, and the plurality of inner panel through holes (41) are arranged in a square area along one side of the inner panel (4).
4. A soil storage and lyophilization sample bottle for microbiological detection according to claim 1, 2 or 3, characterized in that: the soil storage and freeze-drying sample bottle for microorganism detection further comprises a stacking stabilizer (6), and the stacking stabilizer (6) is fixedly connected to the outer side edge of the upper end face of the bottle cap (3).
5. The soil storage and lyophilization sample bottle for microbiological detection according to claim 4, wherein: the stacking stabilizing frame (6) comprises a plurality of limiting arc plates, the limiting arc plates are uniformly distributed along the circumferential direction of the bottle cap (3), and the lower ends of the limiting arc plates are fixedly connected to the outer side edges of the upper end face of the bottle cap (3).
6. A method of testing soil storage and freeze-dried sample bottles for microbiological testing according to any one of claims 1 to 5 comprising the steps of:
step one: firstly, placing a partition plate (2) in a sample storage bottle (1), and uniformly distributing collected soil samples into a plurality of areas separated by the partition plate (2) in the sample storage bottle (1);
step two: two bottle cap inner panels (4) are installed in the bottle cap (3), wherein the inner panel through holes (41) on the upper layer of the bottle cap inner panels (4) are staggered with the bottle cap through holes (31) on the bottle cap (3) at 180 degrees, and the inner panel through holes (41) on the lower layer of the bottle cap inner panels (4) are staggered with the inner panel through holes (41) on the upper layer of the bottle cap inner panels (4) at 180 degrees;
step three: rotating the bottle cap knob (5), rotating the cover plate (51) to the outer side of the bottle cap through hole (31) to enable the bottle cap through hole (31) to be in a closed state, and then covering the bottle cap (3) on the bottle mouth of the sample storage bottle (1);
step four: firstly, placing a sample storage bottle (1) into a refrigerator with the temperature of minus 20 ℃ for pre-freezing, then placing the sample storage bottle (1) into a freeze dryer, rotating a bottle cap knob (5), keeping a cover plate (51) away from a bottle cap through hole (31), enabling the bottle cap through hole (31) to be in an open state, stacking and placing a plurality of sample storage bottles (1) along the height direction under the clamping of a stacking stabilizing frame (6), and after placing, starting the freeze dryer for freeze drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210186032.XA CN114476330B (en) | 2022-02-28 | 2022-02-28 | Soil storage and freeze-drying sample bottle for microorganism detection and detection method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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| Publication number | Publication date |
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| CN114476330A (en) | 2022-05-13 |
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