CN218665996U - B vitamin detection kit - Google Patents
B vitamin detection kit Download PDFInfo
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- CN218665996U CN218665996U CN202223112431.6U CN202223112431U CN218665996U CN 218665996 U CN218665996 U CN 218665996U CN 202223112431 U CN202223112431 U CN 202223112431U CN 218665996 U CN218665996 U CN 218665996U
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Abstract
The utility model discloses a B vitamins detection kit, which comprises an outer packaging body, a reagent storage area and an accessory storage area, wherein the reagent storage area is internally provided with a standard reagent bottle, a culture medium reagent bottle and a sterile water reagent bottle; the accessory storage area is provided with an anaerobic gas generation bag, an anaerobic culture container and a microporous plate; the anaerobic culture container is a sealed box or a sealed bag, the volume of the anaerobic culture container is larger than the sum of the volume of the microporous plate and the volume of the anaerobic gas generation bag, and the microporous plate and the anaerobic gas generation bag can be completely contained in the anaerobic culture container. The utility model discloses the kit can provide more homogeneous stable cultivation space for the microbiological culture process that the microbiological method detected the B vitamins, improves the detection precision of B vitamins.
Description
Technical Field
The utility model belongs to the technical field of food, health products detect, specifically be a B vitamin detect reagent box suitable for microbiological detection method.
Background
The detection of B vitamins in food is an important content of the detection of nutrient elements in food. The vitamin B-dependent microorganisms are used as strains for detection at home and abroad, and the detection is carried out by a microbiological method.
Some of the existing microbiological detection methods are to pre-place the detection strains in a micropore plate by using a freeze-drying method (such as a patent with the publication number of EP1774021A 2), directly add a culture medium and a sample to be detected for use, and use an enzyme-labeling instrument for reading. Similar functions are realized by pre-placing a strain for detection in a 96-well plate by a method of low vacuum environment and room temperature evaporation in serial patents with publication number CN106434840A and the like. Similarly, in the patent publication No. CN204086263A, the microorganism for detection is lyophilized in the form of microspheres into a vial, and the lyophilized microspheres containing the microorganism for detection are dissolved and then added into a 96-well plate for use. A similar method exists in the national standard GB5009.211-2022 determination of folic acid in food.
According to the method for detecting the vitamin B content in the food by using the microbiological method, the used microorganism for detection is usually anaerobe or facultative anaerobe, the culture environment can be isolated from the external environment by using the film covering method, and the anaerobic culture of the independent space in the micropores of the microporous plate is realized along with the growth of the microorganism. The method specifically comprises the following steps: the method is characterized in that a microporous plate is used as a culture and detection container, after a culture is added into the microporous plate, a sticky plastic film or plastic sheet is used for coating a film on the surface of the microporous plate, each micropore forms an independent culture space, and then the growth condition of microorganisms is read after the culture is cultured for a specific time under specific conditions. The disadvantages of this detection method are: due to the adoption of the manual film covering method, the different holes are not uniform in the aspects of tightness, closed space size and the like, so that the growth environments of microorganisms are different, and the growth difference of the microorganisms is larger. In more serious cases, the film is easily stuck insecurely, and air leakage or liquid leakage can be caused in the process of culturing the microorganisms.
Disclosure of Invention
The utility model aims to overcome above technical defect, provide a B family microorganism detect reagent box suitable for microbial detection method, can provide more homogeneous stable cultivation space for the microbial cultivation process before detecting, improve detection stability and detection precision.
The utility model aims at realizing through the following technical scheme:
a B vitamin detection kit comprises an outer packaging body, a reagent storage area and an accessory storage area, wherein the reagent storage area and the accessory storage area are arranged in the outer packaging body, and a standard reagent bottle, a culture medium reagent bottle and a sterile water reagent bottle are placed in the reagent storage area; the accessory storage area is provided with an anaerobic gas generation bag, an anaerobic culture container and a microporous plate; the anaerobic culture container is a sealing box or a sealing bag, the volume of the anaerobic culture container is larger than the sum of the volume of the microporous plate and the volume of the anaerobic gas generation bag, and the microporous plate and the anaerobic gas generation bag can be completely contained in the anaerobic culture container.
As the optimization of the anaerobic culture container, the sealing bag is a plastic sealing bag, and a sealing strip or a sealing zipper is arranged at the opening of the plastic sealing bag.
In another preferred mode of the anaerobic culture container, the box body of the sealing box is made of glass or plastic, and the box cover is made of plastic.
Preferably, the bottom of the microplate is pre-provided with a strain layer for detection.
Another preference is: the reagent storage area is also provided with a microorganism reagent bottle for detection, any one of strain freeze-dried powder for detection, strain freeze-dried microspheres for detection or bacterium liquid for detection is arranged in the microorganism reagent bottle for detection, and no preset strain for detection is arranged in the microporous plate.
Furthermore, the standard reagent bottle, the culture medium reagent bottle and the sterile water reagent bottle are respectively provided with at least 2 bottles, and the microporous plate is provided with at least 2 blocks.
Furthermore, the micropore plate is a 96-hole enzyme label plate.
Furthermore, still deposited sealing film in the district is deposited to the annex, sealing film's area is not less than the area of micropore board upper surface, sealing film's edge is provided with the adhesive part that is used for pasting with the micropore board, the adhesive part outside covers has the adhesive foil that can reveal off.
Further, the outer packing body is a box body with a cover, the reagent storage area is a box body provided with a lining, the lining is a fixing frame with a through hole, the through hole is used for inserting a reagent bottle, and the lining is made of plastic, foam, a hard paper shell or rubber. The accessory storage area is the part of the outer packaging body except the reagent storage area. And the microporous plates, the anaerobic gas generation bags and the anaerobic culture containers are arranged side by side or stacked in the reagent storage area.
Or, the outer packaging body is a box body with a cover, the accessory storage area is a box body without a cover, and the reagent storage area is the part except the accessory storage area in the outer packaging body. Various reagent bottles are directly placed in the reagent storage area. And the microporous plates, the anaerobic gas generation bags and the anaerobic culture containers are arranged side by side or stacked in the reagent storage area.
The utility model has the advantages that:
the utility model discloses a set up anaerobism gassing package and anaerobic culture container in the kit, can replace traditional tectorial membrane to cultivate, for the microbiological method detects B vitamin and provides more homogeneous, stable microorganism culture environment for the detection, improved the stability and the accuracy that detect.
Drawings
FIG. 1 is a schematic sectional view of the vitamin B complex detection kit in example 1;
FIG. 2 is a schematic view showing the structures of an anaerobic gas generating bag, an anaerobic culture vessel and a microplate in example 1;
FIG. 3 is a schematic view showing the usage states of the anaerobic gas generating bag, the anaerobic culture vessel and the microplate in example 1.
The present invention will be further described with reference to the accompanying drawings and examples.
Description of reference numerals:
1. a reagent storage area; 2. an accessory storage area; 1-1. Standard reagent bottle; 1-2, culture medium reagent bottle; 1-3, sterile water reagent bottle;
1-4. Microorganism reagent bottle for detection; 2-1, anaerobic gas production bag; 2-2, anaerobic culture container; 2-3. A micro-porous plate.
Detailed Description
Example 1
As shown in fig. 1 and 2, a B-group vitamin detection kit comprises an outer packaging body (a box body in this embodiment), and a reagent storage area 1 and an accessory storage area 2 arranged inside the outer packaging body, wherein a standard reagent bottle 1-1, a culture medium reagent bottle 1-2, a sterile water reagent bottle 1-3, and a microorganism reagent bottle 1-4 for detection are placed in the reagent storage area 1; the accessory storage area 2 is provided with an anaerobic gas generating bag 2-1, an anaerobic culture container 2-2 and a microporous plate 2-3; as shown in FIGS. 2 and 3, the anaerobic culture vessel 2-2 in this embodiment is a sealed bag having a volume larger than the sum of the volume of the micro well plate 2-3 and the volume of the anaerobic gas generating bag 2-1, and the interior of the sealed bag can completely contain the micro well plate 2-3 and the anaerobic gas generating bag 2-1.
In this embodiment, the sealing bag is a plastic sealing bag, and a sealing zipper is arranged at the opening of the plastic sealing bag. In this embodiment, the microorganism reagent bottle 1-4 for detection is provided with lyophilized detection strain powder, and the microporous plate is not provided with a preset detection strain.
In this example, 3 bottles and 3 microwell plates were provided for each of the microbial reagent bottle for detection, the standard reagent bottle, the medium reagent bottle, and the sterile water reagent bottle. The micropore plate is a 96-hole enzyme label plate.
In this example, a 15cm × 30cm plastic sealed bag was used as an anaerobic culture container, and a 350mL anaerobic gas-generating bag (type: C-11Anaeropouch anaerobic gas-generating bag, mitsubishi, japan, or an Oxoid-cell Anaerogen small-size gas-generating bag) was used in combination with the sealed bag. The sealing bags and anaerobic gas production bags sold in the market are made of different materials and have different specifications, and professional technicians in the industry can flexibly select the anaerobic culture bags and the anaerobic gas production bags matched with the anaerobic culture bags according to needs, so that the detailed description is omitted.
In this embodiment, the outer package body is a box body with a cover, the accessory storage region 2 is a box body without a cover, and the reagent storage region 1 is a portion of the outer package body excluding the accessory storage region 2. A standard reagent bottle 1-1, a culture medium reagent bottle 1-2, a sterile water reagent bottle 1-3 and a microorganism reagent bottle 1-4 for detection are directly arranged in a reagent storage area 1. In the accessory storage area 2, a micro-porous plate 2-3 and an anaerobic gas generating bag 2-1 are arranged at the lower layer side by side, and a plastic sealing bag is arranged at the upper layer of the micro-porous plate 2-3 and the anaerobic gas generating bag 2-1.
The method of use of the kit in this example:
1) Dissolving a test culture medium in a culture medium reagent bottle 1-2 by using sterile water in a sterile water reagent bottle 1-3, carrying out water bath at 95 ℃ for 5 minutes, cooling and filtering, adding the filtered culture medium into a test microorganism freeze-dried substance in a microorganism reagent bottle 1-4 for detection, and uniformly mixing to completely dissolve the culture medium to obtain a test bacteria suspension;
2) Dissolving the standard substance in the standard substance reagent bottle 1-1 by using sterile water in the sterile water reagent bottle 1-3, diluting the standard substance in different proportions to obtain a standard substance diluent, and preparing a standard curve;
3) Processing and preparing a sample suspension according to the type of the sample, and diluting according to the content of the vitamin to be detected in the sample to obtain a sample diluent;
4) Adding a certain volume of test bacterial suspension into the microporous plates 2-3, and then respectively adding a certain volume of standard substance diluent and sample diluent into different wells;
5) Placing the microporous plate 2-3 in an anaerobic culture container 2-2 (a sealed bag), and placing the microporous plate in an anaerobic gas generation bag 2-1, and sealing;
6) Culturing at a certain temperature for a certain time;
7) After the culture is finished, taking the microporous plate 2-3 out of the anaerobic culture container 2-2, and repeatedly blowing and sucking by using a pipettor to uniformly mix the microbial cultures in the micropores;
8) After mixing, reading by using an enzyme-linked immunosorbent assay;
9) After the reading was complete, the test results were analyzed using software.
Example 2
The embodiment is improved on the basis of the embodiment 1, 3 sealing films are additionally arranged on the accessory storage area 2 of the B vitamin detection kit, and other technical characteristics are the same as those of the embodiment 1. The area of sealing film equals the area of micropore board upper surface, the edge of sealing film is provided with the sticky portion that is used for pasting with the micropore board, sticky portion outside covers has the adhesive foil that can reveal.
The method of using the kit in this example, only step 7) differs from example 1: after the culture is finished, taking out the microporous plate 2-3 from the anaerobic culture container 2-2, and covering the microporous plate 2-3 with a sealing film; and repeatedly shaking or oscillating the microporous plate to uniformly mix the microbial cultures in the micropores.
Example 3
In this embodiment, the improvement is made on the basis of embodiment 1, and in the B-vitamin detection kit, the reagent storage area 1 is not provided with detection microorganism reagent bottles 1-4; the microporous plate 2-3 is provided with a preset strain layer for detection.
The method of use of the kit in this example:
1) Dissolving the test culture medium in the culture medium reagent bottle 1-2 in sterile water in the sterile water reagent bottle 1-3, carrying out water bath at 95 ℃ for 5 minutes, cooling, and filtering to obtain a test culture solution;
2) Dissolving the standard substance in the standard substance reagent bottle 1-1 by using sterile water in the sterile water reagent bottle 1-3, diluting the standard substance in different proportions to obtain a standard substance diluent, and preparing a standard curve;
3) Processing and preparing a sample suspension according to the type of the sample, and diluting according to the content of the vitamin to be detected in the sample to obtain a sample diluent;
4) Adding a certain volume of test culture solution into the microporous plate 2-3, and then respectively adding a certain volume of standard product diluent and sample diluent into different wells;
5) Placing the microporous plate 2-3 in an anaerobic culture container 2-2 (a sealed bag), and placing the microporous plate in an anaerobic gas generation bag 2-1, and sealing;
6) Culturing at a certain temperature for a certain time;
7) After the culture is finished, taking the microporous plate 2-3 out of the anaerobic culture container 2-2, and repeatedly blowing and sucking by using a pipettor to uniformly mix the microbial cultures in the micropores;
8) After mixing, reading by using an enzyme-labeling instrument;
9) After the reading was complete, the test results were analyzed using software.
Example 4
In this embodiment, an improvement is made on the basis of embodiment 3, and 3 pieces of sealing films are additionally arranged in the accessory storage area 2 of the B-vitamin detection kit, and other technical features are the same as those in embodiment 3. The area of sealing film equals the area of micropore board upper surface, the edge of sealing film is provided with the sticky portion that is used for pasting with the micropore board, sticky portion outside covers has the adhesive foil that can reveal.
The method of using the kit in this example, only step 7) differs from example 3: after the culture is finished, taking the microporous plate 2-3 out of the anaerobic culture container 2-2, and covering a film on the microporous plate 2-3 by using a sealing film; and repeatedly shaking or oscillating the microporous plate to uniformly mix the microbial cultures in the micropores.
Finally, it should be noted that the above only illustrates the technical solution of the present invention and not by limitation, and although the present invention has been described in detail with reference to preferred arrangements, a person skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention.
Claims (8)
1. The utility model provides a B clan vitamin detect reagent box, deposits district and annex and deposits the district including the internal reagent that sets up of extranal packing body and extranal packing, its characterized in that: a standard reagent bottle, a culture medium reagent bottle and a sterile water reagent bottle are placed in the reagent storage area; the accessory storage area is provided with an anaerobic gas generation bag, an anaerobic culture container and a microporous plate; the anaerobic culture container is a sealed box or a sealed bag, the volume of the anaerobic culture container is larger than the sum of the volume of the microporous plate and the volume of the anaerobic gas generating bag, and the inner space of the anaerobic culture container can completely contain the microporous plate and the anaerobic gas generating bag.
2. The B vitamin assay kit according to claim 1, wherein: the sealing bag is a plastic sealing bag, and a sealing strip or a sealing zipper is arranged at the opening of the plastic sealing bag.
3. The B vitamin assay kit according to claim 1, wherein: the box body material of seal box is glass or plastics, and the lid material is plastics.
4. The B vitamin assay kit according to claim 1, wherein: the bottom of the micropore plate is preset with a strain layer for detection.
5. The B vitamin assay kit according to claim 1, wherein: the reagent storage area is also provided with a microorganism reagent bottle for detection, any one of strain freeze-dried powder for detection, strain freeze-dried microspheres for detection or bacterium liquid for detection is arranged in the microorganism reagent bottle for detection, and a preset strain layer for detection is not arranged in the microporous plate.
6. The B vitamin assay kit according to claim 1, wherein: the standard reagent bottle, the culture medium reagent bottle and the sterile water reagent bottle are respectively provided with at least 2 bottles, and the microporous plate is provided with at least 2 blocks.
7. The B vitamin assay kit according to claim 1, wherein: the micropore plate is a 96-hole enzyme label plate.
8. The B vitamin detection kit according to claim 1, wherein: still deposited sealing film in the annex is deposited the district, sealing film's area is not less than the area of micropore board upper surface, sealing film's edge is provided with the sticky portion that is used for pasting with the micropore board, the sticky portion outside covers has the adhesive foil that can reveal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223112431.6U CN218665996U (en) | 2022-11-23 | 2022-11-23 | B vitamin detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223112431.6U CN218665996U (en) | 2022-11-23 | 2022-11-23 | B vitamin detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218665996U true CN218665996U (en) | 2023-03-21 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202223112431.6U Active CN218665996U (en) | 2022-11-23 | 2022-11-23 | B vitamin detection kit |
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| CN (1) | CN218665996U (en) |
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