CN114469815A

CN114469815A - Common bombax flower extract and preparation method and application thereof

Info

Publication number: CN114469815A
Application number: CN202210179809.XA
Authority: CN
Inventors: 郭朝万; 孙云起; 魏瑞敬; 胡露; 王娟; 蒲艳
Original assignee: Guangdong Marubi Biological Technology Co Ltd
Current assignee: Guangdong Marubi Biological Technology Co Ltd
Priority date: 2022-02-25
Filing date: 2022-02-25
Publication date: 2022-05-13

Abstract

The application relates to the field of skin care product raw materials, in particular to a common bombax flower extract and a preparation method and application thereof. The preparation method of the common bombax flower extract comprises the following steps: extracting the kapok with an extractant, and collecting a liquid phase; the extracting agent comprises the following components in parts by volume: 17-28 parts of betaine, 50-85 parts of glycerol and 0.4-9 parts of propylene glycol. The kapok extract is extracted by adopting the extracting agent, the extracting agent can not be removed after extraction is finished, and the preparation method can obtain more flavone content, improve the extraction rate of the kapok flavone, save the extraction process and improve the oxidation resistance level of the kapok extract.

Description

Common bombax flower extract and preparation method and application thereof

Technical Field

The application relates to the field of skin care product raw materials, in particular to a common bombax flower extract and a preparation method and application thereof.

Background

Kapok mainly contains chemical components such as flavonoids, organic acids, steroids, triterpenes, coumarins and the like.

The common bombax flower flavone can be used as raw material of skin care products, but the common bombax flower flavone extracted by the prior art has limited antioxidant capacity.

Disclosure of Invention

The embodiment of the application aims to provide a common bombax flower extract, and a preparation method and application thereof, which aim to improve the extraction efficiency of common bombax flower flavone.

The application provides a preparation method of a common bombax flower extract, which comprises the following steps:

extracting kapok with an extractant, and collecting a liquid phase;

the extracting agent comprises the following components in parts by volume: 17-28 parts of betaine, 50-85 parts of glycerol and 0.4-9 parts of propylene glycol.

The kapok extract is extracted by adopting the extracting agent, the extracting agent can not be removed after extraction is finished, and the preparation method can obtain more flavone content, improve the extraction rate of the kapok flavone, save the extraction process and improve the oxidation resistance level of the kapok extract.

In some embodiments of the present application, the extractant further comprises water, the volume fraction of water in the extractant being 30-70%;

optionally, the extractant further comprises water, and the water accounts for 55-60% of the volume fraction of the extractant.

In some embodiments of the present application, the ratio of extractant to kapok is (30ml-70 ml): 1g of a compound;

optionally, the ratio of the extractant to the kapok is 40 ml: 1g of the total weight of the composition.

In some embodiments of the present application, the step of extracting kapok with the extractant comprises:

the mixture containing the extractant and the kapok is subjected to high-voltage pulse electric field extraction under the conditions that the pulse voltage is 500-700v and the pulse number is 70-90.

In some embodiments of the present application, the extractant includes a material produced by Hemerocallis, Shenzhen, Hemerocallis, Inc. under the product name supramolecular DES-TG.

In some embodiments of the present application, the extractant is an aqueous solution of supramolecular DES-TG in a concentration of 30-70% by volume.

In some embodiments of the present application, collecting the liquid phase further comprises concentrating the liquid phase under reduced pressure.

The application also provides a kapok extract which is prepared by adopting the preparation method of the kapok extract.

The application also provides an application of the kapok extract in anti-inflammation or anti-skin aging.

The application also provides a skin care product which comprises auxiliary materials and the kapok extract.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained from the drawings without inventive effort.

Fig. 1 shows an external view of test example 1.

FIG. 2 shows a comparison of chick embryo chorioallantoic membranes of PEF-DES kapok extract provided in example 1 before and 5min after dosing.

FIG. 3 shows a comparison of chick embryo chorioallantoic membranes of the ultrasound-assisted extraction of Bombacaceae extract of example 6 before and 5min after dosing.

Fig. 4 shows an appearance of the skin of the cream provided in test example 5 before use.

Fig. 5 shows a skin appearance of the cream provided in test example 5 after 10 days of use.

Fig. 6 shows a skin appearance of the cream provided in test example 5 after 28 days of use.

Fig. 7 is a graph showing wrinkle-removing effects of the cream provided in test example 5 for different use times.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions of the embodiments of the present application will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

The kapok extract of the present application, and the preparation method and application thereof will be specifically described below.

The application provides a preparation method of a kapok extract, which comprises the following steps: extracting kapok with an extractant, and collecting a liquid phase;

Specifically, the volume ratio of betaine, glycerol and propylene glycol is (17-28): (50-85): (0.4-9).

For example, the parts by volume of betaine (chemical name is N, N-trimethylglycine) may be 17 parts, 18 parts, 21 parts, 22 parts, 23 parts, 24 parts, 26 parts, 27 parts, 28 parts, and the like.

For example, the glycerol may be present in 50 parts, 51 parts, 53 parts, 55 parts, 57 parts, 59 parts, 60 parts, 66 parts, 67 parts, 68 parts, 70 parts, 72 parts, 74 parts, 75 parts, 80 parts, 81 parts, 83 parts, 84 parts, 85 parts, and the like, by volume.

For example, the propylene glycol may be present in amounts of 0.4 parts, 0.5 parts, 1.2 parts, 1.5 parts, 2.1 parts, 2.5 parts, 3 parts, 3.2 parts, 4.1 parts, 4.5 parts, 5.2 parts, 5.5 parts, 6.2 parts, 6.5 parts, 7.2 parts, 7.5 parts, 8.1 parts, 8.5 parts, 9 parts, and the like, by volume.

In some embodiments of the present application, the extractant may include only betaine, glycerol, propylene glycol, and unavoidable impurities in the above-described ratios.

It is understood that in other embodiments of the present application, the extractant may also include other solvents that do not react with betaine, glycerol, propylene glycol.

In some embodiments, the extractant further comprises water, the volume fraction of water in the extractant being 30-70%. In other words, the volume of the remaining components of the extractant, excluding water, is equal to 30-70% of the total volume of the extractant. In some embodiments, the volume fraction of water in the extractant is 35-40%. For example, the volume fraction of water in the extractant can be 30%, 31%, 33%, 35%, 36%, 38%, 39%, 40%, 51%, 52%, 55%, 56%, 58%, 59%, 60%, 64%, 65%, 66%, 68%, 69%, 70%, and so forth.

It is understood that in other embodiments of the present application, the extractant may not include water.

It is noted that in some embodiments of the present application, the extractant may be configured as follows:

mixing betaine and ethanol, stirring at room temperature until the solution is uniform, slowly adding into glycerol and propylene glycol, then placing the mixed solution in nitrogen atmosphere at room temperature, magnetically stirring for 8-20h, keeping away from light during the stirring process, and storing at zero centigrade for 10-20 h. Removing ethanol and water by vacuum rotary evaporation to obtain the extractant.

It is understood that the present application is not limited to the preparation method of the extractant, and the extractant may be obtained in a commercially available manner.

For example, in some embodiments of the present application, the extractant is selected from those produced by Shenzhen Hemerocallis Biotech, Inc., under the product name of supramolecular DES-TG. The supermolecule DES-TG contains betaine, glycerol and propylene glycol.

In some embodiments, the extractant is an aqueous solution of supramolecular DES-TG at a concentration of 30-70% by volume, e.g., supramolecular DES-TG may be at a concentration of 30%, 31%, 33%, 35%, 36%, 38%, 39%, 40%, 51%, 52%, 55%, 56%, 58%, 59%, 60%, 64%, 65%, 66%, 68%, 69%, 70% by volume, etc.

In some embodiments, the ratio of extractant to kapok is (30ml-70 ml): 1g of the total weight of the composition. For example, for the embodiment where the extractant is an aqueous solution of supramolecular DES-TG with a volume concentration of 30-70%, the ratio of extractant to kapok is (30ml-70 ml): 1g of the total weight of the composition.

For the examples where the extractant comprises water in a volume fraction of 30-70% of the extractant, the ratio of extractant to kapok is (30ml-70 ml): 1g of the total weight of the composition.

For example, the ratio of kapok to extractant may be 1 g: 32ml, 1 g: 35ml, 1 g: 40ml, 1 g: 45ml, 1 g: 50ml, 1 g: 55ml, 1 g: 60ml, 1 g: 62ml, 1 g: 63ml, 1 g: 65ml, 1 g: 70ml, etc.

It is understood that the dosage ratio of the kapok and the aqueous solution of the extracting agent can be other, and correspondingly, the dosage ratio of the kapok and the extracting agent can be other on the basis of not considering the utilization rate of raw materials and the cost.

In some embodiments, the kapok is extracted using an extraction agent using a high voltage pulsed electric field extraction technique, for example, a mixture comprising the extraction agent and kapok is subjected to high voltage pulsed electric field extraction at a pulse voltage of 500-.

The aforementioned pulse voltage may be 500v, 550v, 600v, 650v, 700v, or the like. The foregoing number of pulses may be 70, 75, 78, 80, 85, 90, etc.

It is understood that in other embodiments of the present application, kapok may be extracted by means of ultrasonic extraction.

After the kapok is extracted with the extractant, the liquid phase is collected, and in the present application, only a part of the liquid phase, for example, the upper liquid phase is collected and the lower solid phase is removed, without considering the yield and the like.

In some embodiments, the separation is performed by filtration, the filter residue is removed, and the filtrate is collected.

In some embodiments of the present application, the method for preparing kapok extract further comprises concentrating the filtrate under reduced pressure, and concentrating the filtrate under reduced pressure to remove a portion of the extractant. It will be appreciated that other methods of treatment, such as freeze drying, may be used for product requirements, or that subsequent concentration and treatment may not be used.

Alternatively, in some other embodiments of the present application, the extractant may not be removed, the extracted solid phase may be removed directly, and all of the liquid phase may be collected.

The preparation method of the kapok extract provided by the application at least has the following advantages:

In light of the above, the kapok extract provided by the application has a high flavone content, and shows excellent anti-inflammatory and antioxidant properties.

Based on the advantages, the application also provides an application of the kapok extract in anti-inflammation or anti-skin aging.

The application also provides a skin care product, which comprises auxiliary materials and any one of the kapok extracts. The auxiliary material can set up according to skin care products's kind and demand, and this application does not restrict it.

The skin care product has good antioxidant and antiinflammatory effects.

The features and properties of the present application are described in further detail below with reference to examples.

In the following examples and comparative examples, DES-TG, DES-TGP, DES-TGN, DES-TPB, and DES-TBN were obtained from Hemerocallis, Dahurian, Shenzhen, Hemerocallis, Inc.

Wherein, the DES-TG mainly comprises glycerol, betaine and propylene glycol.

The main components of DES-TGP are glycerol, betaine, malic acid and propylene glycol.

The main components of DES-TGN are glycerol, betaine, citric acid and propylene glycol.

The main components of DES-TPB are betaine, malic acid and propylene glycol.

The main components of DES-TBN are propylene glycol, citric acid and betaine.

Example 1

This example provides a kapok extract, which is prepared by the following steps:

1) drying and crushing the commercially available kapok, and sieving the crushed kapok with a 30-mesh sieve to obtain powder. Adding water into DES-TG to obtain 40% DES-TG water solution, and making into extractant.

2) Taking 10g kapok powder, and mixing the extract with the kapok powder according to the proportion of 1 g: adding kapok at a ratio of 40ml, and performing high-voltage pulse extraction at a voltage of 600V and a pulse number of 80. And after extraction, carrying out suction filtration to obtain the kapok extract, wherein the kapok extract contains an extracting agent and an extract.

3) Detecting the content of flavone in the common bombax flower extract by using an aluminum nitrate colorimetric method, specifically, the detection method comprises the following steps:

taking 2.4mL of kapok extract diluted to a certain multiple in a test tube with a plug, and then adding 5 wt% of NaNO₂0.4mL of solution is evenly mixed and reacted for 6 min; then 0.4mL of 10 wt% Al (NO) was added₃)₃Fully and uniformly mixing the solution, and reacting for 6 min; and finally, adding 4mL of 4.3 wt% NaOH solution and 2.8mL of 75 vol% ethanol, fully mixing, then carrying out a dark reaction for 15min, and measuring the light absorption value at 510nm of an ultraviolet spectrophotometer (each gradient is parallel to 3). And substituting the corresponding light absorption value into a standard working curve for conversion to obtain the content of the flavone in the kapok extract.

Example 2 to example 5

Examples 2 to 5 each provide an extract of kapok, please refer to example 1, and examples 2 to 5 differ from example 1 in the extractant. In examples 2-5, the extractant was prepared using the following procedure:

mixing betaine and ethanol, stirring at room temperature until the solution is uniform, slowly adding glycerol and propylene glycol, placing the mixed solution in nitrogen atmosphere at room temperature, magnetically stirring for 8h, keeping away from light during the stirring process, and storing at 0 deg.C for 10 h. The ethanol and water were removed by vacuum rotary evaporation to obtain the extractant, the volume ratios of glycerol, propylene glycol and betaine are shown in table 1.

The rest of the preparation method and preparation process were the same as in example 1.

The total flavone extraction rate is calculated according to the following formula:

the total flavone extraction rate Y ═ [ (C × V2)/(V1 × m × 1000) ] × 100%.

Wherein Y: the percentage (%) of total flavonoids extractable from the raw material;

c: calculating the mass concentration (g/L) of total flavonoids in the extractive solution by substituting into a standard curve;

v1: volume of extract (mL) for determination;

v2: the total volume (mL) of the sample extract;

m: is the sample weight (g).

The extraction rate of flavone was measured for the method provided in example 4, and the extraction rate of flavone for the method provided in example 4 was 14.38%.

Example 6

This example provides a kapok extract, and example 6 differs from example 1 in step 2), in this example, 2) 10g of kapok powder was taken, and the ratio of extractant to kapok powder was 1 g: adding kapok at a ratio of 40ml, and performing ultrasonic extraction at an ultrasonic temperature of 60 ℃ and an ultrasonic power of 300W for 30 min; and after extraction, carrying out suction filtration, and concentrating the filtrate under reduced pressure to obtain the kapok extract.

Comparative examples 1 to 21

Comparative examples 1 to 21 each provide an extract of kapok, and referring to example 1, comparative examples 1 to 21 are different from example 1 in the extraction agent. The extractants of comparative examples 1-21 are shown in table 1.

TABLE 1

As can be seen from table 1: the higher flavone content of examples 1-13, compared to other comparative examples, indicates that the method provided herein can yield more kapok flavones.

Test example 1

DES-TG, 1mg/ml vc solution and 3 vol% kapok extract provided in example 1 were mixed with DPPH solution, and after standing for 30min, the system color was observed, as shown in FIG. 1, it can be seen from FIG. 1 that DES-TG has no antioxidant effect, and the kapok extract extracted in example 1 has antioxidant effect.

Experimental example 2

The anti-aging capacity of the kapok extract provided in example 1 was measured using a cell scratch test method.

The test results are shown in table 2.

TABLE 2

As can be seen from table 2, the kapok extract provided in example 1 has a good cell healing effect.

Test example 3

Taking the kapok extract provided in the embodiment 1, and preparing the kapok extract and an aqua auxiliary material into an aqua; mixing with emulsion adjuvant to obtain emulsion; wherein, preservative is not added in the auxiliary materials of the water agent and the emulsion.

The above aqueous solution, emulsion and kapok extract provided in example 1 were each independently left to stand in the following conditions 1 to 5 for 6 months, and the change in appearance was observed.

Condition 1: normal temperature; condition 2: alternating (-18 ℃ to 45 ℃); condition 3: 45 ℃; condition 4: -18 ℃; condition 5: and (5) illumination.

After standing for 6 months under the conditions 1-5, the appearance of the aqua, the emulsion and the kapok extract provided in example 1 is not changed, which indicates that the kapok extract provided in example 1 has better stability.

Test example 4

The kapok extract provided in example 1 was mixed with purified water to prepare a 10% solution, which was added dropwise to the chick embryo chorioallantoic membrane. The pictures of chick embryo chorioallantoic membrane before and after 5min of drug addition are shown in FIG. 2.

The left side of FIG. 2 shows the chick embryo chorioallantoic membrane image before the drug is added, and the right side of FIG. 2 shows the chick embryo chorioallantoic membrane image after the drug is added.

Ultrasonic-assisted alcohol extraction is taken and mixed with pure water to prepare a 10% solution, and the 10% solution is dripped into a chick embryo chorioallantoic membrane. The pictures of chick embryo chorioallantoic membrane before and after 5min of drug addition are shown in FIG. 3.

The left side of FIG. 3 shows the chick embryo chorioallantoic membrane image before the drug is added, and the right side of FIG. 3 shows the chick embryo chorioallantoic membrane image after the drug is added.

As can be seen from fig. 3 and 2, the kapok extract with ultrasound-assisted alcohol extraction is more irritating than that provided in example 1.

Test example 5

The kapok extract provided in example 1 was mixed with a base cream to prepare a cream, and the above-mentioned both sides were subjected to a human body test twice a day, and fig. 4 to 6 are photographs of the cream before use, 10 days, and 28 days, respectively.

FIG. 7 shows measurement of the infraocular wrinkle area using VISIA-CR.

Fig. 4 to 7 show that the kapok extract provided in example 1 has a superior wrinkle removal effect.

The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A preparation method of a kapok extract is characterized by comprising the following steps:

extracting kapok with an extractant, and collecting a liquid phase;

2. The method for preparing kapok extract according to claim 1, wherein the extractant further comprises water, and the volume fraction of water in the extractant is 30-70%;

3. The method for preparing kapok extract according to claim 2, wherein the ratio of the extractant to the kapok is (30ml-70 ml): 1 g;

4. The method of claim 1, wherein the step of extracting kapok with the extractant comprises:

5. The method for preparing kapok extract according to claim 1, wherein the extractant comprises a material produced by shenzhen Hemerocallis Biotech limited under the product name of supramolecular DES-TG.

6. The method for preparing kapok extract according to claim 1, wherein the extractant is an aqueous solution of supramolecular DES-TG with a volume concentration of 30-70%.

7. The method of producing the kapok extract according to any one of claims 1 to 6, further comprising concentrating the liquid phase under reduced pressure after the collecting the liquid phase.

8. A bombax ceiba extract, which is prepared by the method for preparing bombax ceiba extract as claimed in any one of claims 1 to 6.

9. Use of the kapok extract of claim 8 for anti-inflammatory or anti-skin aging.

10. A skin care product comprising an adjuvant and the kapok extract of claim 8.