CN114469773B - Stable anti-aging composition, preparation and application thereof - Google Patents
Stable anti-aging composition, preparation and application thereof Download PDFInfo
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- CN114469773B CN114469773B CN202210142292.7A CN202210142292A CN114469773B CN 114469773 B CN114469773 B CN 114469773B CN 202210142292 A CN202210142292 A CN 202210142292A CN 114469773 B CN114469773 B CN 114469773B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
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- A61K2800/522—Antioxidants; Radical scavengers
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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Abstract
The invention belongs to the technical field of cosmetics. More particularly, it relates to a stable anti-aging composition comprising at least: fermentation product obtained by liquid fermentation of prolamin derived from corn or wheat by at least one microorganism; the fermentation product obtained by the method has excellent oxidation resistance, and the clearance rate of the fermentation product of 6mg/mL to DPPH free radicals is equivalent to the oxidation resistance of VC of 1 mg/mL; the composition can further comprise ferulic acid, has more stable antioxidant performance, shows good antioxidant activity in a wide pH range (slightly acidic and slightly alkaline, pH 3-8), and increases the skin firmness by 32.1-36.9% and reduces the average wrinkle depth of the skin by-29.6-34.3% after 12 weeks of essence prepared by the anti-aging composition; the number of wrinkles is reduced by 21.5-26.4%.
Description
Technical Field
The invention belongs to the technical field of cosmetics. More particularly, it relates to a stable anti-aging composition comprising at least: a fermentation product obtained by liquid fermentation of prolamin derived from corn or wheat by at least one microorganism.
Background
The gliadin is the main storage protein in corn and wheat, wherein the Zein (Zein) molecule contains a large-area alpha-spirochete which is formed by the hydrogen bond action of hydroxyl and imino on a peptide main chain, so that the gliadin has stronger hydrophobicity. The zein contains antioxidant amino acid functional sequences with SOD-like activity, such as Gln-Gln-Pro-Gln-Pro-Trp, phe-Pro-Leu-Glu-Met-Pro-Phe, leu-Asp-Tyr-Glu, tyr-Ala, his-Cys-Met-Leu, etc. A large amount of research data show that the hydrolysate of zein has certain antioxidant performance, and the current preparation method of zein hydrolysate comprises enzymolysis, chemical modification and microbial fermentation; for example Jingshan san [2] And the zein with stronger antioxidant activity is obtained by fermenting the corn gluten meal with the bacillus natto.
However, at present, aspergillus niger is not used for fermenting zein and the antioxidant activity of the obtained fermentation product in different pH ranges is not researched.
Disclosure of Invention
The present invention provides a stable anti-aging composition comprising at least: fermentation product obtained by liquid fermentation of prolamin derived from corn or wheat by at least one microorganism. The fermentation product has strong antioxidant activity in a wide pH range.
It is an object of the present invention to provide a stable anti-aging composition comprising at least: fermentation product obtained by liquid fermentation of prolamin derived from corn or wheat by at least one microorganism. The composition exhibits a surprising antioxidant capacity, 6mg/ml of fermentation product being comparable to a VC solution with a mass concentration of 1.0mg/ml for DPPH radical scavenging. The composition is useful for improving the signs of aging of the skin, such as increasing the firmness, elasticity, and/or luster of the skin while reducing wrinkles.
The microorganism described herein is aspergillus niger, different strains of which ferment prolamins under different conditions, resulting in fermentation products that exhibit different physiological activities. In some cases, single-strain fermentation or dual-strain co-fermentation may be selected. In the research process, the product performance obtained by adopting aspergillus niger fermentation is considerable, compared with the non-fermented alcohol soluble protein, the product performance has obvious improvement on the scavenging effect of free radicals, and compared with other strains, the antioxidant capacity is also obviously improved.
The results of antioxidant tests show that the clearance rate of the fermentation product with the mass concentration of 6mg/mL on DPPH and hydroxyl free radical is equivalent to the antioxidant capacity of VC with the mass concentration of 1.0 mg/mL.
Further, the invention defines the prolamin as zein.
In some cases, 0.1 to 1 wt.% ferulic acid may also be included in the anti-ageing composition according to the invention, and it has surprisingly been found that the composition exhibits an appreciable antioxidant stability compared to VC, which has been shown by tests to exhibit good antioxidant activity over a wide pH range (pH 3 to 8).
Further, the preparation of the fermentation product comprises:
adding prolamin derived from corn or wheat into a basic culture medium to obtain a fermentation culture medium, inoculating aspergillus niger into the fermentation culture medium, fermenting for 72-90 hours at the temperature of 30-35 ℃ under the condition of 150-300 r/min, collecting fermentation supernatant, and filtering to obtain the corn or wheat gliadin.
In some cases, it is possible to use,the basic culture medium is a conventional culture medium suitable for the growth of aspergillus niger, and specifically, the fermentation culture medium adopted in the invention is as follows: 1 to 5 percent of yeast extract and KH 2 PO 4 0.01～0.03％、CaCl 2 0.1-0.5 percent of dextrin, 0.5-2 percent of dextrin and the balance of deionized water, and the pH value is 7.0.
In some cases, corn or wheat derived prolamins are added to a basal medium at 5-20% by weight to obtain a fermentation medium; preferably 5 to 15% by weight, 5 to 10% by weight; more preferably 5% by weight, 8% by weight, 10% by weight, 12% by weight, 15% by weight are added.
In some cases, aspergillus niger seed solution is inoculated into the fermentation medium at a volume ratio of 1 to 12%.
In some cases, aspergillus niger seed solutions are prepared by conventional techniques, i.e., inoculating Aspergillus niger into the seed medium and culturing on a shaker overnight. The seed culture medium and the culture conditions are as follows: 1 to 2 percent of dextrin, 2 to 5 percent of yeast extract, 1 to 3 percent of ammonium chloride and KH 2 PO 4 0.01～0.03％、CaCl 2 0.2 to 0.5 percent, the balance of deionized water, pH7.0, the culture temperature of 32 to 37 ℃,160 to 180r/min, and the culture time of 24 hours.
The invention also aims to provide the application of the composition in preparing a stable anti-aging preparation, wherein the composition shows stable antioxidant capacity and stronger antioxidant capacity in a wider pH range (3-8).
The good antioxidant capacity is realized by that the composition has excellent scavenging effect on DPPH free radicals, and tests show that the scavenging rate of the DPPH free radicals is 92.6% in 6mg/ml composition.
Another object of the present invention is to provide a stable anti-aging essence, which comprises:
1-15% by weight of a fermentation supernatant obtained by liquid fermentation of a prolamin derived from corn or wheat by at least one microorganism;
0.1 to 1 percent of ferulic acid.
In some cases, the serum comprises:
5-15% of a fermentation supernatant obtained by liquid fermentation of corn or wheat derived prolamin by at least one microorganism;
0.5 to 1 percent of ferulic acid.
In some cases, the serum comprises:
10-15% of a fermentation supernatant obtained by liquid fermentation of zein derived from corn or wheat by at least one microorganism;
0.5 to 1 percent of ferulic acid.
In some cases, the serum comprises:
fermentation supernatant obtained by liquid fermentation of 12% prolamin derived from corn or wheat by at least one microorganism;
0.1% by weight of ferulic acid.
In some cases, the anti-aging serum further comprises:
1 to 3% by weight of a thickener;
1 to 20% by weight of alcohols;
1-3% by weight of an emulsifier;
0.01 to 0.1% by weight of a preservative;
1-3% of glycerol; and
the balance of water.
In some cases, the thickener is preferably a natural thickener, an organic semi-synthetic thickener, an organic synthetic thickener, or a combination thereof; more preferably a combination of organic natural thickeners including but not limited to xanthan gum, guar gum and organic semi-synthetic thickeners; organic semisynthetic thickeners include, but are not limited to: hydroxymethyl cellulose, hydroxyethyl cellulose, PEG-120 methyl glucose dioleate.
In some cases, the thickener is PEG-120 methyl glucose dioleate, and the weight of PEG-120 methyl glucose dioleate in the serum is 1%, 2%, 3%.
In some cases, the alcohols are alcohols commonly used in the cosmetic art, including but not limited to: 1.3-propanediol, 1,3-butanediol, 1,2-pentanediol, 1,2-hexanediol, butanediol. Preferably, the alcohol is 1,3-butanediol, and the weight of 1,3-butanediol in the essence is preferably 5-20%, 10-20% and 15-20%; in some embodiments, the weight of 1,3-butanediol is 15%, 20%.
In some cases, the emulsifier may be selected from fatty alcohol polyoxyethylene ethers, polyethylene glycol fatty acid esters, glycerolipids, silicone oils, sorbitols, saccharide derivatives, and the like.
In some cases, fatty alcohol polyoxyethylene ether emulsifiers include, but are not limited to: laureth-4, laureth-8, laureth-23, steareth-2, steareth-21, nonylpheneth-10, nonylpheneth-40, ceteareth-6, ceteareth-12, ceteareth-25, ceteareth-30, ceteareth-50, ceteth-20, isocetyl-20 and steareth-20.
In some cases, glycerolipid emulsifiers include, but are not limited to: glyceryl isostearate, glyceryl stearate citrate, PEG-20 glyceryl stearate, and PEG-30 glyceryl stearate.
In some cases, silicone oil-based emulsifiers include, but are not limited to: PEG-12 polydimethylsiloxane, PEG9 methyl ether polydimethylsiloxane, PEG-11 methyl ether polydimethylsiloxane, lauryl PEG-8 polydimethylsiloxane, lauryl PEG-9 polydimethylsiloxane ethyl polydimethylsiloxane, polyglycerol-3 disiloxane polydimethylsiloxane.
In some cases, sorbitol-based emulsifiers include, but are not limited to: sorbitan laurate, sorbitan palmitate, sorbitan stearate, sorbitan oleate, sorbitan sesquioleate, sorbitan isostearate, polysorbate-20, polysorbate-40, polysorbate-60, polysorbate-80, and polysorbate-85.
In some cases, saccharide derivative emulsifiers include, but are not limited to: c14-22 alcohols, C12-20 alkyl glucosides, coco glucoside, coco alcohol, cetearyl alcohol, coco glucoside, cetearyl alcohol, cetearyl glucoside, sucrose stearate, sucrose distearate.
In some cases, preservatives include, but are not limited to: phenoxyethanol, potassium sorbate, chlorphenesin and methylparaben.
The invention has the following beneficial effects:
(1) According to the invention, the Aspergillus niger is adopted to ferment the zein to obtain the fermentation product with excellent oxidation resistance, and tests show that the scavenging capacity of the fermentation product with the mass concentration of 6mg/mL on DPPH free radicals is equivalent to that of VC with the mass concentration of 1.0 mg/mL.
(2) The invention also provides a composition which is prepared by combining the fermentation product and ferulic acid, the composition shows amazing stable antioxidant performance, and shows good antioxidant capacity in a wide pH range (slightly acidic and slightly alkaline, pH 3-8), and essence prepared by using the anti-aging composition can increase skin firmness and reduce wrinkles after 12 weeks, and specifically, the skin firmness is increased by 32.1-36.9%, and the average wrinkle depth of the skin is reduced by-29.6-34.3%; the number of wrinkles is reduced by 21.5-26.4%.
Drawings
FIG. 1 is a graph comparing the effect of DPPH radical scavenging by each sample.
Detailed Description
The present invention is further illustrated by the following specific examples, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
Test I, fermentation Strain screening test
Aspergillus niger and Bacillus subtilis are respectively adopted to ferment zein, and the antioxidant activity of the fermentation product is inspected.
1.1 test methods
1.1.1 Aspergillus niger fermentation: the Aspergillus niger seed culture medium and the culture conditions are as follows: dextrin 1%, yeast extract 3%, ammonium chloride 1%, KH 2 PO 4 0.01％、CaCl 2 0.2 percent of the rest deionized water, the pH value is 7.0, the culture temperature is 35 ℃, and the culture time is 160r/min and 24 hours; adding 12% by weight of Zein into an Aspergillus niger basic culture medium, inoculating Aspergillus niger seed liquid into the Aspergillus niger basic culture medium according to the volume ratio of 10%, fermenting for 84h at 34 ℃ under the condition of 150r/min, collecting fermentation supernatant, filtering, decoloring and deodorizing by using active carbon, and filtering to obtain a Zein/Aspergillus niger fermentation product (ZA); the basic culture medium comprises: yeast extract 3% and KH 2 PO 4 0.02％、CaCl 2 0.2 percent of dextrin, 1 percent of deionized water and the balance of pH7.0.
1.1.2 fermentation of Bacillus subtilis: the bacillus subtilis seed culture medium and the culture conditions are as follows: culturing peptone 1.0%, yeast extract 2%, sodium chloride 1.0%, and deionized water in balance at 37 deg.C and 120r/min for 24 hr; adding 12% by weight of Zein into a bacillus subtilis basal culture medium, inoculating bacillus subtilis seed liquid into the culture medium according to the volume ratio of 10%, fermenting for 84h at 36 ℃ under the condition of 100r/min, collecting fermentation supernatant, filtering, decoloring and deodorizing by using activated carbon, and filtering to obtain a Zein/bacillus subtilis fermentation product (ZB); every 1000ml of bacillus subtilis basic culture medium comprises: 10g of peptone, 3g of beef extract, 5g of NaCl, pH 7.3-7.5
1.2 comparison of antioxidant activity:
1.2.1DPPH radical clearance assay: respectively preparing sample solutions with the pH of 7.0,1-6 mg/ml by using ethanol solutions for a Zein/aspergillus niger fermentation product, a Zein/bacillus subtilis fermentation product and untreated Zein; respectively putting 2ml of sample solutions with different concentrations into a test tube, adding 2ml of 0.1mol/L DPPH solution (prepared by 95% ethanol solution), mixing, shaking up, and incubating for 30min at room temperature in a dark place. Determination of the Absorbance A at a wavelength of 517nm 1 The absorbance A was measured by using an equal volume of ethanol solution as a blank 0 . Meanwhile, the DPPH free radical scavenging rate was calculated according to the following formula using VC solution (prepared in distilled water) with a mass concentration of 1.0mg/ml as a positive control test group, and the results are shown in Table 1 and FIG. 1.
1.3 results and analysis
1.3.1 Effect on DPPH radical scavenging
Table 1:
the test shows that the scavenging rate of VC solution with the mass concentration of 1.0mg/ml to DPPH free radicals is 89.2%, as can be seen from Table 1 and figure 1, the scavenging rates of DPPH free radicals of ZA, ZB and Zein are obviously enhanced along with the increase of the mass concentration of the two, and in the three, the scavenging capacity of ZA to DPPH free radicals under the same mass concentration is obviously higher than that of ZB and Zein, and when the mass concentration of ZA is 6mg/ml, the scavenging capacity of ZA free radicals is slightly higher than that of VC solution with the mass concentration of 1.0 mg/ml.
Test example two antioxidant Activity at different pH
Respectively preparing ZA sample liquid with the mass concentration of 6mg/ml, ZB sample liquid with the mass concentration of 6mg/ml and ferulic acid solution with the mass concentration of 0.1mg/ml by using 75% ethanol solution for later use, adjusting and preparing to obtain sample liquid with different pH values, and then testing 6mg/ml ZA +0.1mg/ml FA according to the test method of 1.2 in the first test example; antioxidant activity of 6mg/ml ZB +0.1mg/ml FA at various pH values, with 6mg/ml ZA and 0.1mg/ml FA solutions as controls, the results of the tests are shown in Table 2.
TABLE 2 comparison of DPPH radical scavenging Effect at different pH
Analyzing the table 2, the pH range of the sample 6mg/ml ZA +0.1mg/ml FA in the range of 3.0-8.0 reaches more than 85% of DPPH free radicals, and the sample shows good antioxidant performance in a wide pH range, and the stability of the antioxidant performance after compounding ZB and FA with the same concentration is lower than that of compounding ZA and FA; the anti-oxidation activity of FA in an acidic environment is better than that of a weakly alkaline condition, probably because the FA in the acidic environment has better stability; ZA has an unsatisfactory antioxidant activity under acidic and slightly alkaline conditions, and the surprising antioxidant stability of the composition is realized by the synergistic effect of the ZA and FA.
EXAMPLE 1 Zein/Aspergillus niger fermentation product preparation
The Aspergillus niger seed culture medium and the culture conditions are as follows: dextrin 1%, yeast extract 3%, ammonium chloride 1%, KH 2 PO 4 0.01％、CaCl 2 0.2 percent of the rest deionized water, the pH value is 7.0, the culture temperature is 35 ℃, and the culture time is 160r/min and 24 hours;
adding 12% by weight of Zein into an Aspergillus niger basic culture medium, inoculating Aspergillus niger seed liquid into the culture medium according to the volume ratio of 10%, fermenting for 84h at 34 ℃ under the condition of 150r/min, collecting fermentation supernatant, filtering, decoloring and deodorizing by using active carbon, and filtering to obtain a Zein/Aspergillus niger fermentation product (ZA); the basic culture medium comprises: yeast extract 3% and KH 2 PO 4 0.02％、CaCl 2 0.2 percent of dextrin, 1 percent of deionized water and the balance of pH7.0.
EXAMPLE 2 Zein/Aspergillus niger fermentation product preparation
The Aspergillus niger seed culture medium and the culture conditions are as follows: dextrin 1%, yeast extract 3%, ammonium chloride 1%, KH 2 PO 4 0.01％、CaCl 2 0.2 percent of the rest deionized water, the pH value is 7.0, the culture temperature is 35 ℃, and the culture time is 160r/min and 24 hours;
adding 10% by weight of Zein into an Aspergillus niger basic culture medium, inoculating Aspergillus niger seed liquid into the culture medium according to the volume ratio of 8%, fermenting for 72 hours at 35 ℃ under the condition of 200r/min, collecting fermentation supernatant, filtering, decoloring and deodorizing by using active carbon, and filtering to obtain a Zein/Aspergillus niger fermentation product (ZA); the basic culture medium comprises: 5% of yeast extract and KH 2 PO 4 0.01％、CaCl 2 0.1 percent of dextrin, 0.5 percent of rest deionized water, and the pH value is 7.0.
EXAMPLE 3 Zein/Aspergillus niger fermentation product preparation
Aspergillus niger seed culture mediumAnd the culture conditions are as follows: dextrin 1%, yeast extract 3%, ammonium chloride 1%, KH 2 PO 4 0.01％、CaCl 2 0.2 percent, the balance being deionized water, the pH value being 7.0, the culture temperature being 35 ℃,160r/min, and the culture time being 24 hours;
adding 15% by weight of Zein into an Aspergillus niger basic culture medium, inoculating Aspergillus niger seed liquid into the culture medium according to the volume ratio of 12%, fermenting for 90 hours at the temperature of 30 ℃ under the condition of 300r/min, collecting fermentation supernatant, filtering, decoloring and deodorizing by using active carbon, and filtering to obtain a Zein/Aspergillus niger fermentation product (ZA); the basic culture medium comprises: 2% of yeast extract and KH 2 PO 4 0.03％、CaCl 2 0.2 percent of dextrin, 2 percent of rest deionized water, and the pH value is 7.0.
EXAMPLE 4 Zein/Aspergillus niger fermentation product preparation
The Aspergillus niger seed culture medium and the culture conditions are as follows: dextrin 1%, yeast extract 3%, ammonium chloride 1%, KH 2 PO 4 0.01％、CaCl 2 0.2 percent, the balance being deionized water, the pH value being 7.0, the culture temperature being 35 ℃,160r/min, and the culture time being 24 hours;
adding 8% by weight of Zein into an Aspergillus niger basic culture medium, inoculating Aspergillus niger seed liquid into the culture medium according to the volume ratio of 5%, fermenting for 90 hours at 32 ℃ under the condition of 150r/min, collecting fermentation supernatant, filtering, decoloring and deodorizing by using active carbon, and filtering to obtain a Zein/Aspergillus niger fermentation product (ZA); the basic culture medium comprises: 1.5% of yeast extract and KH 2 PO 4 0.03％、CaCl 2 0.2 percent of dextrin, 2 percent of rest deionized water, and the pH value is 7.0.
Examples 5 to 8 anti-aging essence
The preparation method comprises the following steps:
adding deionized water into a stirring pot, starting stirring, adding ferulic acid, glycerol and 1,3-butanediol, heating to 80-85 ℃, and preserving heat for 30min for later use; adding laureth-23 and PEG-120 methyl glucose dioleate into another stirring pot, heating to 80-85 deg.C, and keeping the temperature for use; sieving the two obtained materials by a screen mesh, pumping into an emulsifying pot, adding a Zein/Aspergillus niger fermentation product, starting stirring, homogenizing and emulsifying for 8-10 min; slowly cooling to 45 deg.C after emulsification, adding phenoxyethanol, stirring, and discharging after inspection.
Evaluation of anti-aging efficacy
120 female volunteers were randomly divided into 4 groups of 30 persons, and skin elasticity (R) before and after 12 weeks of use was measured on subjects using a skin elasticity tester MPA580 (manufactured by Courage + Khaz aka (CK) of Germany) 2 Value), and analyzing skin firmness changes; the appearance of both sides of the corner of the eye before and after 12 weeks of use of the subject was measured and analyzed using a skin optical three-dimensional measurement system (PRIMOS, GFMesstechnik inc.) to obtain the results of the average depth of wrinkles and the number of wrinkles as shown in table 3 below.
Table 3 skin firmness, average depth of wrinkles, number of wrinkles test results
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (4)
1. Stable anti-aging composition, characterized in that it comprises at least: 1 to 15 weight percent of zein is obtained by liquid fermentation of Aspergillus niger, and 0.1 to 1 weight percent of ferulic acid.
2. The composition of claim 1, wherein the production of the fermentation product comprises:
adding zein into a basic culture medium to obtain a fermentation culture medium, inoculating Aspergillus niger into the fermentation culture medium, fermenting for 72 to 90 hours under the conditions of 30 to 35 ℃ and 150 to 300r/min, collecting fermentation supernatant, and filtering to obtain the corn zein.
3. Use of a composition according to claim 1 or 2 for the preparation of a stable anti-ageing formulation.
4. A stable anti-aging essence, which comprises:
1 to 15 percent of zein by weight is subjected to liquid fermentation by aspergillus niger to obtain fermentation supernatant;
0.1 to 1% by weight of ferulic acid;
1~3% by weight of a thickener;
1 to 20% by weight of an alcohol;
1~3% by weight of an emulsifier;
0.01 to 0.1 percent by weight of preservative;
1~3% glycerol; and
the balance of water;
wherein the alcohol is selected from the group consisting of 1, 3-propanediol, 1,3-butanediol, 1,2-pentanediol, and 1,2-hexanediol.
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