CN114467411A

CN114467411A - Nano seed coating emulsion and its preparing method

Info

Publication number: CN114467411A
Application number: CN202210031999.0A
Authority: CN
Inventors: 张定兵; 张世乾; 刘婕仪; 魏亚; 陈晓枫; 杨立平; 李广泽; 景辉; 曹明章
Original assignee: Shenzhen Noposion Agrochemicals Co Ltd
Current assignee: Shenzhen Noposion Agrochemicals Co Ltd
Priority date: 2022-01-12
Filing date: 2022-01-12
Publication date: 2022-05-13

Abstract

The application belongs to the technical field of pesticide preparations, and particularly relates to a nano seed coating emulsion and a preparation method thereof. The nano seed coating emulsion comprises the following raw materials in parts by weight based on 100 parts of the total weight of the nano seed coating emulsion: 1-10 parts of pesticide active ingredient, 1-20 parts of organic solvent, 1-20 parts of emulsifier, 1-5 parts of medium/trace elements, 1-5 parts of biostimulant, 1-2 parts of film-forming agent and the balance of water. The nano seed coating emulsion provided by the application has the advantages that the particle size of the emulsion in the emulsion system is basically in a nano level, the particle size is small, the distribution uniformity is high, the stability of the nano seed coating emulsion system is improved, and the coating treatment effect of the seed coating emulsion on seeds is also improved. The growth of the seeds in the seedling stage is well promoted, and the characteristics of uniform seedling emergence, green seedlings, strong seedlings and the like of the seeds are improved; and has no phytotoxicity influence in the vegetative growth period and the reproductive growth period of the whole plant.

Description

Nano seed coating emulsion and its preparing method

Technical Field

The application belongs to the technical field of pesticide preparations, and particularly relates to a nano seed coating emulsion and a preparation method thereof.

Background

The seed coating agent is developed on the basis of seed dressing agent and seed soaking agent, and has the greatest advantage that a layer of firmer film pesticide preparation can be formed outside seeds, so that the seed coating agent is named. The seed coating agent is used for coating seeds, so that the standardization, pelleting and commercialization of improved seeds can be promoted, the quality of the seeds can be improved after the seeds are coated with the seed coating agent, the uniform, complete and strong emergence of the seedlings can be guaranteed, and the seeds can be saved; the seed coating can also prevent and treat diseases such as insect, bird, rat and nutrient deficiency, the seed coating is sowed in the soil, and the seed coating forms a barrier for protecting the seed around the seed, so that the seed is disinfected and pathogenic bacteria infection is prevented and treated. Meanwhile, the method has the functions of saving time, labor, seeds and labor and improving economic and ecological benefits.

At present, the seed coating agent which is the mainstream is still the suspended seed coating agent, but the suspended seed coating agent system belongs to a metastable state in a thermodynamically stable system. According to the Stokes sedimentation law, the suspended seed coating often has the problems of precipitation layering, flatulence, particle size distribution deviation, large particle size and the like, is not beneficial to seed absorption and has long sanding time. In addition, the suspended seed coating agent generally has high viscosity, the seed coating agent packing material is retained with the liquid medicine, and the liquid medicine is unevenly distributed, so that the seeds are easy to generate key factors, and unnecessary economic loss is caused. Therefore, the invention of a novel nano seed coating emulsion is imminent and is rarely seen in the prior art.

Disclosure of Invention

The application aims to provide a nano seed coating emulsion and a preparation method thereof, and aims to solve the problems that the existing suspension seed coating agent is large in particle size, uneven in distribution on the surface of seeds and not beneficial to seed absorption to a certain extent.

In order to achieve the purpose of the application, the technical scheme adopted by the application is as follows:

in a first aspect, the application provides a nano seed coating emulsion, which comprises the following raw material components in parts by mass based on 100 parts by mass of the nano seed coating emulsion: 1-10 parts of pesticide active ingredient, 1-20 parts of organic solvent, 1-20 parts of emulsifier, 1-5 parts of medium/trace elements, 1-5 parts of biostimulant, 1-2 parts of film-forming agent and the balance of water.

Further, the emulsifier comprises at least one of isooctanol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylaryl polyoxypropylene polyoxyethylene ether and alkylaryl polyoxypropylene polyoxyethylene ether derivatives.

Further, the organic solvent comprises at least one of nitrogen octyl pyrrolidone, pelargonide, nitrogen octyl pyrrolidone derivatives and pelargonide derivatives.

Further, the organic solution comprises the nitrogen octyl pyrrolidone and/or the nitrogen octyl pyrrolidone derivative and the pelargonide and/or the pelargonide derivative, wherein the ratio of the total mass of the nitrogen octyl pyrrolidone and/or the nitrogen octyl pyrrolidone derivative to the total mass of the pelargonide and/or the pelargonide derivative is (1-9): 1.

further, the medium/trace elements comprise at least one of manganese, magnesium, boron, zinc and molybdenum.

Further, the biological stimulin comprises at least one of potassium fulvate, polyglutamic acid, aspartic acid and corn polysaccharide.

Further, the pesticide active ingredient comprises at least one of metalaxyl-M, fludioxonil, hymexazol and difenoconazole.

Further, the film forming agent comprises at least one of polyethylene glycol, cellulose derivatives and polyurethane derivatives.

Further, the biostimulant comprises the polyglutamic acid and the corn polysaccharide in a mass ratio of 1: 1.

Further, the particle size of the nano seed coating emulsion is 4.6-240 nm; the particle size D90 of the nano seed coating emulsion is 180-200 nm.

In a second aspect, the present application provides a method for preparing a nano seed coating emulsion, comprising the following steps:

taking the total mass of the nano seed coating emulsion as 100 parts, mixing 1-10 parts of pesticide active ingredients, 1-20 parts of organic solvents, 1-20 parts of emulsifiers, 1-5 parts of medium/trace elements, 1-5 parts of biostimulant, 1-2 parts of film-forming agents and the balance of water to obtain the nano seed coating emulsion.

Further, the step of mixing process comprises:

dissolving the pesticide active ingredient in the organic solvent, and then sequentially adding the emulsifier and the film-forming agent mixed solvent to obtain an organic premix;

dissolving the trace elements and the biostimulant in water to obtain an aqueous solution premix;

and mixing the organic premixed solution with the aqueous solution premixed solution to obtain the nano seed coating emulsion.

According to the nano seed coating emulsion provided by the first aspect of the application, a seed coating system is in a stable emulsion state through the synergistic effect of the emulsifier and the organic solvent, and the particle size of the traditional Chinese medicine in the seed coating emulsion is basically in a nano level, the particle size is small and the distribution uniformity is high. Not only improves the stability of the nano seed coating emulsion system, but also improves the coating treatment effect of the seed coating emulsion on seeds. In addition, the nano seed coating emulsion contains functional components such as medium/trace elements, biological stimulin and the like, has a good promotion effect on the growth of seeds in the seedling stage, and improves the characteristics of uniform seedling emergence, green seedlings, strong seedlings and the like of the seeds; and has no phytotoxicity influence in the vegetative growth period and the reproductive growth period of the whole plant.

According to the preparation method of the nano seed coating emulsion provided by the second aspect of the application, all the components are fully mixed and dissolved to form a stable nano seed coating emulsion state, and then the nano seed coating emulsion is obtained. The preparation process is simple, the operation is simple and convenient, and the method is suitable for industrial large-scale production and application. In the prepared nano seed coating emulsion, the original drug particle size is basically in a nano level, the particle size is small, the distribution uniformity is high, and the stability of a nano seed coating emulsion system and the coating treatment effect on seeds are improved. Through the combined action of the components of the nano seed coating emulsion, the seed coating emulsion has more outstanding disease prevention effect on seeds, has better promotion effect on the growth of the seeds in the seedling stage, and improves the characteristics of uniform seedling emergence, green seedlings, strong seedlings and the like of the seeds. And has no phytotoxicity influence in the vegetative growth period and the reproductive growth period of the whole plant.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the embodiments or the prior art descriptions will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

FIG. 1 is a particle size distribution diagram of the nano seed coating emulsion provided in example 1 of the present application;

FIG. 2 is a graph showing the particle size distribution of the emulsion for suspending seed coating provided in comparative example 1 of the present application;

fig. 3 is a safety test of the nano seed coating emulsion provided in example 1 of the present application on wheat, corn and peanut seeds.

Detailed Description

In order to make the technical problems, technical solutions and advantageous effects to be solved by the present application more clearly apparent, the present application is further described in detail below with reference to the embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present application and are not intended to limit the present application.

In this application, the term "and/or" describes an association relationship of associated objects, meaning that there may be three relationships, e.g., a and/or B, which may mean: a is present alone, A and B are present simultaneously, and B is present alone. Wherein A and B can be singular or plural. The character "/" generally indicates that the former and latter associated objects are in an "or" relationship.

In the present application, "at least one" means one or more, "a plurality" means two or more. "at least one of the following" or similar expressions refer to any combination of these items, including any combination of the singular or plural items. For example, "at least one (one) of a, b, or c," or "at least one (one) of a, b, and c," may each represent: a, b, c, a-b (i.e., a and b), a-c, b-c, or a-b-c, wherein a, b, and c may be single or plural, respectively.

It should be understood that, in various embodiments of the present application, the sequence numbers of the above-mentioned processes do not mean the execution sequence, some or all of the steps may be executed in parallel or executed sequentially, and the execution sequence of each process should be determined by its function and inherent logic, and should not constitute any limitation to the implementation process of the embodiments of the present application.

The terminology used in the embodiments of the present application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used in the examples of this application and the appended claims, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise.

The weight of the related components mentioned in the description of the embodiments of the present application may not only refer to the specific content of each component, but also represent the proportional relationship of the weight among the components, and therefore, the content of the related components is scaled up or down within the scope disclosed in the description of the embodiments of the present application as long as it is scaled up or down according to the description of the embodiments of the present application. Specifically, the mass in the description of the embodiments of the present application may be in units of mass known in the chemical industry, such as μ g, mg, g, and kg.

The terms "first" and "second" are used for descriptive purposes only and are used for distinguishing purposes such as substances from one another, and are not to be construed as indicating or implying relative importance or implying any number of technical features indicated. For example, a first XX may also be referred to as a second XX, and similarly, a second XX may also be referred to as a first XX, without departing from the scope of embodiments of the present application. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature.

The first aspect of the embodiments of the present application provides a nano seed coating emulsion, which comprises the following raw material components in parts by mass, based on 100 parts by mass of the nano seed coating emulsion: 1-10 parts of pesticide active ingredient, 1-20 parts of organic solvent, 1-20 parts of emulsifier, 1-5 parts of medium/trace elements, 1-5 parts of biostimulant, 1-2 parts of film-forming agent and the balance of water.

The nano seed coating emulsion provided by the first aspect of the embodiment of the application comprises 1-10 parts of pesticide active ingredients, 1-20 parts of organic solvents, 1-20 parts of emulsifiers, 1-5 parts of medium/trace elements, 1-5 parts of biostimulant, 1-2 parts of film forming agents, the balance of water and other components, and a seed coating system is in a stable emulsion state through the synergistic effect of the emulsifiers and the organic solvents, and emulsion in the seed coating emulsion is basically in a nano level, small in particle size and high in distribution uniformity. Not only improves the stability of the nano seed coating emulsion system, but also improves the coating treatment effect of the seed coating emulsion on seeds. In addition, the nano seed coating emulsion contains functional components such as medium/trace elements, biological stimulin and the like, has a good promotion effect on the growth of seeds in the seedling stage, and improves the characteristics of uniform seedling emergence, green seedlings, strong seedlings and the like of the seeds; and has no phytotoxicity influence in the vegetative growth period and the reproductive growth period of the whole plant.

The embodiment of the application provides the dosage proportion of each raw material component in the nano seed coating emulsion, which not only ensures the improvement of seed quality by the seed coating emulsion, but also ensures the uniform, complete and strong seedling emergence of seeds; but also ensures that the seed coating agent system is in a nano-particle size emulsion state, so that the seed coating emulsion system has small and uniform particle size distribution and better treatment effect on seeds. If the content of the emulsifier in the nano seed coating emulsion is too high, the nano seed coating emulsion system is changed from milky white to semitransparent and then to a transparent state, and finally an aqueous solution is formed. If the content of the emulsifier in the nano seed coating emulsion is too low, the nano seed coating emulsion cannot be in a stable emulsion state with the nano-scale particle size. If the content of the organic solvent is too high or too low, the formation of a stable nanoemulsion system is also not facilitated. If the contents of the active ingredients of the pesticide, the medium/trace elements, the biological stimulants and the like are too low, the promotion effect on the sprouting and the growth of the seeds is weak, and the effect is not good; too high a content is also not conducive to the formation of a stable nanoemulsion system. If the content of the film forming agent is too high or too low, the nano seed coating emulsion is not favorable for forming a coating layer with uniform thickness and complete coating on the surface of the seeds, and the effect of the seed coating emulsion on the seeds is reduced.

In some embodiments, the emulsifier comprises at least one of isooctanol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylaryl polyoxypropylene polyoxyethylene ether, and derivatives of alkylaryl polyoxypropylene polyoxyethylene ether. The emulsifiers adopted in the embodiment of the application do not contain forbidden nonyl phenol groups, are green and safe and are easy to obtain; the seed coating emulsion is favorable for forming an emulsion state with nano particle size, the stability of a seed coating emulsion system is improved, the seed coating emulsion is favorable for uniformly coating the surface of the seed to form a coating layer with uniform thickness, and the treatment effect of the seed coating emulsion on the seed is improved.

In some embodiments, the organic solvent comprises at least one of nitrogen octyl pyrrolidone, pelargonide, nitrogen octyl pyrrolidone derivatives, pelargonide derivatives. The organic solvents adopted in the embodiment of the application not only avoid the damage of strong polar solvents to seeds, but also avoid the environmental pollution and the harm to operators; the seed coating agent has a good dissolving effect on components such as pesticide active ingredients, and the seed coating agent system is in a nano emulsion state through the matching of the seed coating agent and the emulsifier. Simultaneously, the stability and the safety of the nano seed coating emulsion are ensured.

In some preferred embodiments, the organic solution comprises nitrogen octyl pyrrolidone and/or nitrogen octyl pyrrolidone derivatives and pelargonide and/or pelargonide derivatives, wherein the ratio of the total mass of the nitrogen octyl pyrrolidone and/or nitrogen octyl pyrrolidone derivatives to the total mass of the pelargonide and/or pelargonide derivatives is (1-9): 1. the embodiment of the application adopts a compound organic solvent, wherein the pelargoniamide and the derivatives thereof have better dissolving effect on the active ingredients of the pesticide; and the azooctyl pyrrolidone and the derivative thereof have higher safety system for seeds, and the stability of the nano seed coating emulsion and the safety for the seeds are better ensured by compounding the azooctyl pyrrolidone and the derivative thereof. In some embodiments, the organic solution comprises (1-8) by mass: 1, azooctyl pyrrolidone and derivatives thereof, and pelargonide and derivatives thereof; further, the mass ratio is (2-7): 1; further, the mass ratio is (3-6): 1; further, the mass ratio is (4-5): 1, etc.

In some embodiments, the medium/trace elements comprise at least one of manganese, magnesium, boron, zinc, molybdenum. The trace elements are beneficial to improving the germination of seeds and the generation of plants, wherein the boron element can promote cell division and growth, influence the growth of root systems, regulate the metabolism of indoleacetic acid and ensure that plant growing points grow normally when the supply of boron is sufficient. Manganese is an indispensable nutrient element for plant growth, can participate in various metabolic reactions in the plant body, and magnesium can make the growing leaves of the germinated seeds more dark green and participate in chlorophyll synthesis. The molybdenum element can participate in nitrogen metabolism in plants, promote absorption and transportation of phosphorus, and play an important role in transportation of carbohydrates. In some embodiments, the medium/trace elements include manganese, magnesium, boron, zinc and molybdenum, and the medium/trace elements also include the trace elements, so that the germination of seeds and the growth of plants can be better promoted. In addition, the addition form of the medium/trace elements in the nano seed coating emulsion is not particularly limited in the embodiments of the present application, and the medium/trace elements may be added in the form of a metal salt, or in the form of a complex such as EDTA.

In some embodiments, the biostimulant comprises at least one of potassium fulvate, polyglutamic acid, aspartic acid, corn polysaccharide, wherein the polyglutamic acid is capable of moisturizing and promoting mineral absorption by the seed; the corn polysaccharide has the main functions of increasing the fibrous roots, lateral roots and root hairs of plants in the seedling stage and improving the utilization rate of the fertilizer. The potassium fulvate has the effects of fixing nitrogen, dissolving phosphorus and activating potassium, particularly has obvious synergistic effect on potassium fertilizer, and plays roles in increasing roots, strengthening seedlings, resisting continuous cropping, resisting diseases and improving crop quality. And can strengthen the adhesive force and the rapid absorption capacity of the plant root system, and particularly has obvious effect on physiological diseases caused by lack of trace elements. Meanwhile, the fertilizer can improve the granular structure of soil, loosen the soil, improve the water and fertilizer retention capacity of the soil, adjust the pH value, reduce the content of heavy metals in the soil and reduce the harm of salt ions to seeds and seedlings. Aspartic acid can enhance seed germination, protein synthesis, and nitrogen supply during stressed growth; the growth of root system is enhanced, which is a precursor for synthesizing plant endogenous hormone polyamine, and the salt stress resistance of crops is improved. In some embodiments, the biological stimulants comprise polyglutamic acid and corn polysaccharide in a mass ratio of 1:1, and the two biological stimulants are used for better promoting seed germination and improving seed survival and growth efficiency.

In some embodiments, the film forming agent comprises at least one of polyethylene glycol, a cellulose derivative and a polyurethane derivative, and the film forming agents can improve the film forming effect of the nano seed coating emulsion on the surface of the seed, so that the seed coating emulsion forms a uniform-thickness complete coating layer on the surface of the seed, and the treatment effect of the seed coating emulsion on the seed is improved.

The pesticide active ingredients in the nano seed coating emulsion can be reasonably added according to the application requirements of actual crops, so that the nano seed coating emulsion meets the application requirements of different crop seeds. In some embodiments, the pesticide active ingredient comprises at least one of metalaxyl-M, fludioxonil, hymexazol and difenoconazole, wherein the metalaxyl-M is highly effective against downy mildew, phytophthora, and pythium-causing crop diseases of vegetables, fruit trees, tobacco, oil plants, cotton, grain and the like. Fludioxonil can prevent and control stinking smut, downy mildew, banded sclerotial blight, root rot, take-all, glume blight and straw smut of wheat; barley stripe disease, net blotch, smut; maize bacterial wilt, stem rot, damping off; cotton rhizoctonia rot, red rot, anthracnose, black root, seed rot; soybean peanut damping off and root rot (caused by fusarium); rice bakanae disease, leaf spot of flax, early leaf blast and damping off; black spot and black shank of rape; potato damping off, scab; vegetable blight, anthracnose, brown spot, gummy stem blight. Is suitable for crops such as wheat, barley, corn, cotton, soybean, peanut, rice, rape, potato, vegetables and the like. Hymexazol is a systemic bactericide and soil disinfectant, is absorbed by soil after entering the soil and is combined with inorganic metal salt ions such as iron, aluminum and the like in the soil, effectively inhibits the germination of spores and the normal growth of pathogenic fungi mycelia or directly kills germs, and the pesticide effect can reach two weeks. Hymexazol can be absorbed by plant root and moved in root system, and can be metabolized in plant to produce two kinds of glucoside, which has effect of improving physiological activity of crop, thereby promoting plant growth, tillering root, increasing root hair and improving root activity. Because the influence on bacteria and actinomycetes except pathogenic bacteria in the soil is small, the compound has no influence on the ecology of microorganisms in the soil, can be decomposed into compounds with low toxicity in the soil, and is safe to the environment. The difenoconazole has a broad bactericidal spectrum and has lasting protective and therapeutic effects on ascomycetes, basidiomycetes and semiacquired diseases including alternaria, ascochyta, neurospora, colletotrichum, sphacelotheca, phoma, septoria and cladosporium, powdery mildew, rust fungales and certain species-borne pathogenic bacteria. Has good effect on grape anthracnose and white rot. The seed treatment can improve the yield of crops and ensure the quality of the crops.

In some embodiments, the nano seed coating emulsion has a particle size of 4.6-240 nm, and the nano seed coating emulsion has a particle size D90 of 180-200 nm. The nano seed coating emulsion has centralized particle size distribution, mainly takes less than 200 nanometers as a main part, has small particle size and high uniformity, and has better coating effect on seeds, thereby improving the treatment effect of the nano seed coating emulsion on the seeds. In some embodiments, the particle size D90 of the nano seed coating emulsion is 180-190 nm, 190-200 nm, etc.

In some embodiments, the nano seed coating emulsion can be dissolved by adding warning color, so that the prepared nano seed coating emulsion has specific color, the recognition rate of the nano seed coating emulsion on the seed treatment effect is improved, and the application is more convenient.

The nano seed coating emulsion of the embodiment of the application can be prepared by the following method.

In a second aspect, the embodiment of the present application provides a method for preparing a nano seed coating emulsion, comprising the following steps:

s10, mixing 1-10 parts of pesticide active ingredients, 1-20 parts of organic solvents, 1-20 parts of emulsifiers, 1-5 parts of medium/trace elements, 1-5 parts of bio-stimulin, 1-2 parts of film-forming agents and the balance of water based on 100 parts of the total mass of the nano seed coating emulsion to obtain the nano seed coating emulsion.

According to the preparation method of the nano seed coating emulsion provided by the second aspect of the embodiment of the application, 1-10 parts of pesticide active ingredient, 1-20 parts of organic solvent, 1-20 parts of emulsifier, 1-5 parts of medium/trace elements, 1-5 parts of bio-stimulant, 1-2 parts of film-forming agent and the balance of water are mixed and treated, so that the components are fully mixed and dissolved to form a stable nano seed coating emulsion state, and the nano seed coating emulsion is obtained. The preparation process is simple, the operation is simple and convenient, and the method is suitable for industrial large-scale production and application. In the prepared nano seed coating emulsion, the particle size is basically in a nano level, the particle size is small, the distribution uniformity is high, and the stability of a nano seed coating emulsion system and the coating treatment effect on seeds are improved. Through the combined action of the components of the nano seed coating emulsion, the seed coating emulsion has more outstanding disease prevention effect on seeds, has better promotion effect on the growth of the seeds in the seedling stage, and improves the characteristics of uniform seedling emergence, green seedlings, strong seedlings and the like of the seeds. And has no phytotoxicity influence in the vegetative growth period and the reproductive growth period of the whole plant.

In some embodiments, in the step S10, the step of mixing processing includes:

s11, dissolving the pesticide active ingredients in an organic solvent, and sequentially adding an emulsifier and a film-forming agent mixed solvent to obtain an organic premix;

s12, dissolving the trace elements and the biological stimulin in water to obtain an aqueous solution premix;

and S13, mixing the organic premixed solution with the aqueous solution premixed solution to obtain the nano seed coating emulsion.

According to the characteristics of the raw material components, the pesticide active ingredients are dissolved in the organic solvent respectively to be fully dissolved to form stable organic premixed liquid, the trace elements and the biostimulant are dissolved in the water to be fully dissolved to form aqueous solution premixed liquid, then the organic premixed liquid and the aqueous solution premixed liquid are mixed, and the organic premixed liquid and the aqueous solution premixed liquid are fully mixed and emulsified to obtain the nano seed coating emulsion. By the preparation method, the solubility of each raw material component can be improved, and the emulsion is more favorable for emulsifying to form a seed coating emulsion state with nano particle size. Ensuring the stability and small particle size of the nano seed coating emulsion, thereby improving the seed treatment effect of the nano seed coating emulsion.

In some embodiments, in step S12, a warning color may be further added for dissolving, so that the prepared nano seed coating emulsion has a specific color, the recognition rate of the nano seed coating emulsion on the seed treatment effect is improved, and the application is more convenient.

In order to make the above implementation details and operations of the present application clearly understood by those skilled in the art and to make the progress of the nano seed coating emulsion and the preparation method thereof obviously apparent, the above technical solution is illustrated by a plurality of examples below.

Example 1

A1.5% metalaxyl-M + 2.5% fludioxonil nanometer seed coating emulsion is prepared by the following steps:

weighing 1.6g of metalaxyl-M and 2.6g of fludioxonil, dissolving in 15g of nitrogen octyl pyrrolidone, and stirring and dissolving to obtain a uniform system A;

secondly, adding 24g of octanol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylaryl polyoxypropylene polyoxyethylene ether into the A according to the proportion of 2:3:7, and uniformly stirring to obtain B;

thirdly, adding 1g of film-forming agent polyurethane derivative under stirring to obtain C, dissolving 2 parts of corn polysaccharide, 2 parts of boric acid and 3 parts of warning color by using the balance of water, and uniformly stirring to obtain D;

and fourthly, slowly pouring the D into the C under the stirring condition, and fully stirring to obtain the nano seed coating emulsion for coating the seeds.

Example 2

A2% fludioxonil nanocoating emulsion, which is prepared by the following steps:

weighing 2.1g of fludioxonil, dissolving the fludioxonil in 10g of nitrogen octyl pyrrolidone and 2g of pelargonide, and stirring and dissolving to obtain a uniform system A;

secondly, adding 22g of octanol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylaryl polyoxypropylene polyoxyethylene ether into the A according to the proportion of 2:3:6, and uniformly stirring to obtain B;

③ adding 2g of film-forming agent under stirring to obtain C, dissolving 2 parts of compound amino acid, 2 parts of EDTA-mg and 4 parts of warning color by using the balance of water, and uniformly stirring to obtain D;

Example 3

A3% difenoconazole nanometer seed coating emulsion is prepared by the following steps:

weighing 3.1g of difenoconazole, dissolving in 13g of azooctyl pyrrolidone and 2g of pelargonide, and stirring and dissolving to obtain a uniform system A;

secondly, adding octanol polyoxyethylene ether and 24g of castor oil polyoxyethylene ether into the A according to the proportion of 1:5, and uniformly stirring to obtain B;

dissolving 2 parts of polyglutamic acid, 2 parts of EDTA-mg and 4 parts of warning color by using the balance of water, and uniformly stirring to obtain C;

and fourthly, slowly pouring the C into the B under the stirring condition, and fully stirring to obtain the nano seed coating emulsion for coating the seeds.

Example 4

A1.5% metalaxyl-M + 2.5% hymexazol nanometer seed coating emulsion is prepared by the following steps:

weighing 1.6g of metalaxyl-M and 2.6g of hymexazol, dissolving in 15g of nitrogen octyl pyrrolidone, stirring and dissolving to obtain a uniform system A;

secondly, adding 15g of octyl alcohol polyoxyethylene ether and 15g of alkylaryl polyoxypropylene polyoxyethylene ether into the mixture A according to the proportion of 1:4, and uniformly stirring to obtain B;

③ adding 1g of film-forming agent polyethylene glycol under stirring to obtain C; dissolving 2 parts of potassium fulvate, 2 parts of EDTA-Zn and 4 parts of warning color by using the balance of water, and uniformly stirring to obtain D;

Comparative example 1

A suspended seed coating agent of 1.5 percent metalaxyl-M and 2.5 percent fludioxonil is prepared by the following steps:

will 1.6g metalaxyl-M, 2.6g fludioxonil and the moist dispersant of 3g polycarboxylate, the moist dispersant of 3g phosphate, 1g magnesium aluminium silicate, 4g ethylene glycol, 2g film-forming agent and 0.2g antiseptic and surplus water are all added in the sanding jar that is equipped with 80ml zirconium pearl, the sanding in-process cools off the sanding jar, the prevention is sanded the in-process and is heated up, filter the zirconium pearl and obtain semi-manufactured goods after sanding 1.5h, call semi-manufactured goods quality, because the sanding has the quality loss can't be retrieved completely, then according to semi-manufactured goods quality: 1.5% of xanthan gum mother liquor by mass: adding 14:3:3 mass of color paste, firstly adding 1.5% of xanthan gum mother liquor into the semi-finished product for shearing to uniformly disperse the xanthan gum, preventing water segregation during standing, finally adding the color paste, and fully stirring to obtain the suspended seed coating agent.

Further, in order to verify the progress of the examples of the present application, the following tests were performed on the nano seed coating emulsion prepared in the examples:

1. the particle sizes of the 1.5% metalaxyl-M + 2.5% fludioxonil nano seed coating emulsion prepared in the example 1 and the 1.5% metalaxyl-M + 2.5% fludioxonil suspended seed coating prepared in the comparative example 1 are respectively analyzed and determined, and the test results are shown in the attached

drawings

1 and 2, wherein the particle size of the emulsion prepared in the example 1 is mainly distributed in the range of 4.6-240 nm, the particle size D90 is 191nm, the particle size D50 is 123nm, the emulsion is basically in a nano grade, the particle size is small, the distribution uniformity is high, and the treatment effect of the nano seed coating emulsion on seeds is improved. The suspended seed coating prepared in the comparative example 1 has the particle size of 0.0046-7.566 μm, the particle size D90 of 2.831 μm and the particle size D50 of 0.239 μm, is large in particle size, is basically micron-sized particles, is wide in distribution and has poor seed treatment effect.

2. Testing safety of nano seed coating emulsion to seeds

The paper rolling method is adopted to verify the safety, and after the nano seed coating emulsion prepared in the embodiment 1 is diluted by 500 times, a paper rolling safety verification test is carried out on wheat, corn and peanut coated seeds. And clear water without seed coating product treatment was used as a control group CK. The test results are shown in FIG. 3: the experiments of wheat, corn and peanut paper rolling prove that the nano seed coating emulsion has no influence on the initial germination rate of seeds, and the paper rolling experiment can obviously show that the nano seed coating emulsion has the advantage of obviously promoting the overground and underground growth of the seeds in the early stage.

3. Verifying the coating uniformity of the nano seed coating emulsion on the surface of the seeds

The prepared nano seed coating emulsion is diluted by 500 times by taking the example 2 as a test object, wheat, corn and peanut seeds are respectively treated under the same condition, the coating uniformity is verified, the test seed is 500g and is divided into 10 parts, 30 seeds are taken from each part, each group is repeated three times, and the coating uniformity experimental method refers to the method in DB 11/T739-2020. Then adding 20ml ethanol into the seeds, covering and soaking for 1h, shaking and extracting for 15min, and centrifuging to obtain supernatant. And (3) according to the surface drug loading of the coated seeds and the response range of a spectrophotometer to the seed surface medicament, measuring the absorbance at the maximum absorption wavelength, and taking ethanol as a reference. The test results are shown in table 1 below:

TABLE 1

Species of crop	Wheat (Triticum aestivum L.)	Corn (corn)	Peanut
				Uniformity of first coating	95.5	94.8	94.2
The second coating uniformity%	94.6	94.6	93.6
				Uniformity of coating for the third time%	95.3	93.9	93.3
Average degree of uniformity%	95.1	94.4	93.7

According to the test results, the nano seed coating emulsion prepared by the embodiment of the application has higher coating uniformity on different types and sizes of seeds such as wheat, corn and peanut, the average uniformity value reaches more than 93.7%, the coating effect is good, and the promotion effect on the seeds can be realized in an all-round and stable manner.

4. And verifying the coating falling rate of the nano seed coating emulsion on the surface of the seeds.

After the nano seed coating emulsion prepared in example 2 is diluted by 500 times, wheat, corn and peanut seeds are respectively treated under the same conditions, the adopted experimental instrument is a German Heubach dust content measuring instrument (falling-off experimental instrument), the number of the test seeds is 500g, and the rotating speed of a sample cavity is set as follows: the 40r/min air flow rate was set as: the 12L/min samples are repeated three times each time, and the falling rate of the samples in the group is determined by measuring the dust content in the filter after each measurement. The test results are shown in table 2 below:

TABLE 2

Species of crop	Wheat (Triticum aestivum L.)	Corn (corn)	Peanut
				The first coating falling rate%	4.75	4.86	5.11
The second coating falling rate%	5.12	5.12	5.14
				The third coating falling rate%	4.86	5.05	5.21
Average falling rate%	4.91	5.01	5.15

According to the test results, the nano seed coating emulsion prepared in the embodiment of the application has high coating stability on different types of seeds such as wheat, corn and peanut, the coating falling rate is low after coating, the average value of the coating falling rate is as low as 4.91%, and the formed coating is tightly combined with the seeds, has good stability, is suitable for the seeds of different types and sizes, and is wide in application range.

5. Verifying the control effect of the nano seed coating emulsion on stem root rot/root rot in the growth period of corn and peanut

Coating the experimental object of example 3 according to the drug species ratio of 500:1, and naturally drying; randomly taking soil in a field, sterilizing the soil overnight at 105 ℃ in an oven, taking the soil out, adding deionized water to moisten the soil until the humidity of the soil is consistent with the humidity of the field, uniformly mixing the wheat stem rot strain and the peanut root rot strain which are cultured indoors with moistened soil to carry out soil inoculation treatment, respectively obtaining the soil infected by the stem rot strain and the soil infected by the root rot strain, then sowing CK seeds and seeds coated by the nano seed coating agent, setting 3 repetitions for each treatment, setting 3 blanks for each repetition/CK seed for 5 pots, and counting the prevention effect of each crop target bacteria in the growth period. The specific control effect is shown in the following table 3:

TABLE 3

Crop target bacteria	The first control effect%	The second control effect%	The third control effect%	Mean control effect%
					Wheat stem base rot	88.9	90.3	91.2	90.1
CK	65.2	63.5	64.3	64.3
					Root rot of peanut	89.5	87.7	90.5	89.2
CK	63.4	62.7	61.9	62.7

According to the test results, after the nano seed coating emulsion prepared in the embodiment of the application is used for treating seeds, the defense effect of the seeds on wheat stem-root rot strains and peanut root rot strains is obviously improved, the average prevention effect can reach 90.1%, and the control effect is obviously superior to that of a control group CK for treatment.

The above description is only exemplary of the present application and should not be taken as limiting the present application, as any modification, equivalent replacement, or improvement made within the spirit and principle of the present application should be included in the protection scope of the present application.

Claims

1. The nano seed coating emulsion is characterized by comprising the following raw materials in parts by mass based on 100 parts by mass of the nano seed coating emulsion: 1-10 parts of pesticide active ingredient, 1-20 parts of organic solvent, 1-20 parts of emulsifier, 1-5 parts of medium/trace elements, 1-5 parts of biostimulant, 1-2 parts of film-forming agent and the balance of water.

2. The nano seed coating emulsion of claim 1, wherein the emulsifier comprises at least one of isooctanol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylaryl polyoxypropylene polyoxyethylene ether derivatives.

3. The nano seed coating emulsion of claim 1 or 2, wherein the organic solvent comprises at least one of nitrogen octyl pyrrolidone, pelargonide, nitrogen octyl pyrrolidone derivatives, and pelargonide derivatives.

4. The nano seed coating emulsion of claim 3, wherein the organic solution comprises the nitrogen octyl pyrrolidone and/or the nitrogen octyl pyrrolidone derivative and the pelargonide and/or the pelargonide derivative, wherein the ratio of the total mass of the nitrogen octyl pyrrolidone and/or the nitrogen octyl pyrrolidone derivative to the total mass of the pelargonide and/or the pelargonide derivative is (1-9): 1.

5. the nanocoating emulsion of any one of claims 1, 2 or 4, wherein the medium/trace element comprises at least one of manganese, magnesium, boron, zinc, molybdenum.

6. The nano seed coating emulsion of claim 5, wherein the bio-stimulant comprises at least one of potassium fulvate, polyglutamic acid, aspartic acid, corn polysaccharide;

and/or the pesticide active ingredient comprises at least one of metalaxyl-M, fludioxonil, hymexazol and difenoconazole;

and/or the film forming agent comprises at least one of polyethylene glycol, cellulose derivatives and polyurethane derivatives.

7. The nano seed coating emulsion of claim 6, wherein the biostimulant comprises the polyglutamic acid and the corn polysaccharide in a mass ratio of 1: 1.

8. The nano seed coating emulsion of any one of claims 1, 2, 4, 6 to 7, wherein the particle size of the nano seed coating emulsion is 4.6 to 240 nm;

the particle size D90 of the nano seed coating emulsion is 180-200 nm.

9. The preparation method of the nano seed coating emulsion is characterized by comprising the following steps of:

10. The method for preparing nano seed coating emulsion of claim 9, wherein the step of mixing treatment comprises: