CN114456944B - Aspergillus tubingensis, composite microbial agent containing aspergillus tubingensis and application of aspergillus tubingensis - Google Patents
Aspergillus tubingensis, composite microbial agent containing aspergillus tubingensis and application of aspergillus tubingensis Download PDFInfo
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Abstract
The invention relates to the technical field of microorganisms, in particular to aspergillus tubingensis, a compound microbial agent containing the aspergillus tubingensis and application of the compound microbial agent. The compound microbial agent comprises: aspergillus tubingensis ZN210, bacillus subtilis ZR-2 and Trichoderma cephamatum ZN105. The collection number of the aspergillus tubingensis ZN210 is CGNCC No.22412, the collection number of the bacillus subtilis ZR-2 is CGMCC No.21124, and the collection number of the trichoderma reesei ZN105 is CGNCC No.22411. The aspergillus tubingensis ZN210 provided by the invention has the effect of preventing and controlling cucumber fusarium wilt, the effect of preventing and controlling cucumber fusarium wilt is further improved after the compound microbial agent is prepared, the organic matter decomposition efficiency can be effectively improved, and the compound microbial agent can be used for producing bio-organic fertilizer in secondary composting, so that the cost of agricultural production is reduced, the quality of the organic fertilizer is improved, and the high yield of crops is realized by preventing diseases.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to aspergillus tubingensis, a compound microbial agent containing the aspergillus tubingensis and application of the compound microbial agent.
Background
Biological organic fertilizer is widely applied to agricultural production as an emerging microbial fertilizer. Several studies indicate that the inhibition effect on indigenous pathogenic bacteria after the application of the bio-organic fertilizer to crops is even better than that of the direct application of the microbial fertilizer, and the growth promotion effect on the crops is also better than that of the application of the organic fertilizer, and the bio-organic fertilizer has double effects in agricultural production and is greatly dependent on the characteristics of functional microorganisms. Ceramic et al point out that the characteristics and effective quantity of functional bacteria are the guarantee of the quality of the bio-organic fertilizer product. Therefore, whether the character stability and the higher effective quantity of the functional bacteria in the preparation and long-term storage processes can be ensured is an important problem related to the quality of the bio-organic fertilizer product.
Inoculating functional bacteria with nitrogen fixation, phosphate dissolution, potassium dissolution and disease resistance into the decomposed organic fertilizer and performing transient secondary fermentation is one of the main modes for preparing the biological organic fertilizer. However, the stability of the quality of the bio-organic fertilizer is closely related to the stability of the microbial community structure thereof. Compared with the traditional microbial fertilizer, the background microbial community structure of the decomposed organic fertilizer is more complex, and the bacterial community diversity at the decomposing stage after the functional bacteria are inoculated is changed. Therefore, research shows whether the microbial community structure and the related physicochemical indexes of the functional microbial inoculum are changed or not, and the method has important theoretical and practical guiding significance for improving the production process of the bio-organic fertilizer and improving the quality of the product. However, most of the existing researches focus on the application effect of the bio-organic fertilizer, and few researches on the secondary fermentation process are performed.
Disclosure of Invention
In order to solve the technical problems in the prior art, the invention provides aspergillus tubingensis, a compound microbial agent containing the aspergillus tubingensis and application of the compound microbial agent.
In a first aspect, the invention provides a aspergillus tubingensis (Aspergillus tubingensis) ZN210, the deposited information of which is as follows:
the preservation number is: CGMCC No.22412; the classification is named: aspergillus tubingensis ZN210; the preservation units are as follows: china general microbiological culture Collection center (China Committee for culture Collection); the preservation address is: the institute of microorganisms of national academy of sciences of China, national institute of sciences, no. 1, no. 3, north Chen West Lu, the Korean region of Beijing; the preservation date is: 2021, 4 and 22 days.
The aspergillus tubingensis ZN210 has remarkable capability of preventing and treating cucumber fusarium wilt.
Further, the invention provides a compound microbial agent comprising the aspergillus tubingensis (Aspergillus tubingensis) ZN 210.
Further, the compound microbial agent also comprises bacillus subtilis and trichoderma polyspora.
Further, the bacillus subtilis is bacillus subtilis (Bacillus subtilis) ZR-2, and the preservation information is as follows:
the preservation number is: CGMCC No.21124; the classification is named: bacillus subtilis Bacillus subtilis; the preservation units are as follows: china general microbiological culture Collection center (China Committee for culture Collection); the preservation address is: the institute of microorganisms of national academy of sciences of China, national institute of sciences, no. 1, no. 3, north Chen West Lu, the Korean region of Beijing; the preservation date is: 11/6/2020.
Further, the trichoderma reesei is trichoderma reesei (Trichoderma polysporum) ZN105, and the preservation information is as follows:
the preservation number is: CGMCC No.22411; the classification is named: trichoderma reesei ZN105; the preservation units are as follows: china general microbiological culture Collection center (China Committee for culture Collection); the preservation address is: the institute of microorganisms of national academy of sciences of China, national institute of sciences, no. 1, no. 3, north Chen West Lu, the Korean region of Beijing; the preservation date is: 2021, 4 and 22 days.
Further, the composite microbial agent comprises:
aspergillus tubingensis (Aspergillus tubingensis) ZN 210.0X10 4 -2.0×10 5 cfu/mL, bacillus subtilis (Bacillus subtilis) ZR-2.0X10 9 -8.0×10 9 cfu/mL and Trichoderma reesei (Trichoderma polysporum) ZN105 1.0X10 4 -6.0×10 5 cfu/mL。
Further, the composite microbial agent comprises:
aspergillus tubingensis (Aspergillus tubingensis) ZN 210.5X10 5 -2.0×10 5 cfu/mL, bacillus subtilis (Bacillus subtilis) ZR-2.0X10 9 -6.0×10 9 cfu/mL and Trichoderma reesei (Trichoderma polysporum) ZN105 2.0X10 5 -4.0×10 5 cfu/mL。
The invention further provides application of the aspergillus tubingensis (Aspergillus tubingensis) ZN210 or the compound microbial agent in secondary composting of organic materials, such as composting of livestock manure, plant straw or sludge.
Further, the application is to promote organic matter decomposition.
The invention further provides application of the aspergillus tubingensis (Aspergillus tubingensis) ZN210 or the compound microbial agent in preventing and treating cucumber fusarium wilt.
The invention has the following beneficial effects:
the invention provides aspergillus tubingensis ZN210 which has good capability of controlling cucumber fusarium wilt, and the control efficiency is close to 70%. Further, after aspergillus tubingensis ZN210 is prepared into the microbial agent (combined with trichoderma reesei bacillus subtilis ZR-2 and trichoderma reesei ZN 105), the obtained composite microbial agent has the advantages of improving the decomposition efficiency of organic materials and effectively improving the capability of preventing and treating cucumber fusarium wilt. The compound microbial agent provided by the invention can be used for producing the bio-organic fertilizer in secondary composting, so that the cost of agricultural production is reduced, the quality of the organic fertilizer is improved, and meanwhile, the high yield of crops is realized by preventing diseases.
Drawings
FIG. 1 is a schematic diagram showing the difference in temperature change after inoculating a composite microbial inoculant in the secondary fermentation process of compost provided in example 10 of the present invention;
fig. 2 is a schematic diagram showing the difference of the moisture change after inoculating the composite microbial agent in the secondary fermentation process of the compost provided in embodiment 10 of the invention.
Detailed Description
The following examples are illustrative of the invention and are not intended to limit the scope of the invention.
Example 1
The embodiment provides a aspergillus tubingensis microbial inoculum, the microbial content is as follows:
aspergillus tubingensis ZN 210.0X10 6 cfu/mL。
The invention screens out the best stable symbiotic strain combination from 263 different genera and species, and screens out high-yield strains through independent culture, wherein a strain aspergillus tubingensis ZN210 (Aspergillus tubingensis) with the capability of controlling cucumber fusarium wilt is obtained, and the strain aspergillus tubingensis ZN210 is preserved, and the preservation information is as follows:
the preservation number is: CGMCC No.22412; the classification is named: aspergillus tubingensis ZN210; the preservation units are as follows: china general microbiological culture Collection center (China Committee for culture Collection); the preservation address is: the institute of microorganisms of national academy of sciences of China, national institute of sciences, no. 1, no. 3, north Chen West Lu, the Korean region of Beijing; the preservation date is: 2021, 4 and 22 days.
Example 2
The embodiment provides a composite microbial agent, which comprises the following components:
further, the optimal stable symbiotic bacterial combination screened by the invention also comprises bacillus subtilis ZR-2 (Bacillus subtilis) and trichoderma polyspora ZN105 (Trichoderma polysporum).
The preservation information of the trichoderma reesei ZN105 is as follows:
the preservation number is: CGMCC No.22411; the classification is named: trichoderma reesei ZN105; the preservation units are as follows: china general microbiological culture Collection center (China Committee for culture Collection); the preservation address is: the institute of microorganisms of national academy of sciences of China, national institute of sciences, no. 1, no. 3, north Chen West Lu, the Korean region of Beijing; the preservation date is: 2021, 4 and 22 days.
The preservation information of the bacillus subtilis ZR-2 is as follows:
the preservation number is: CGMCC No.21124; the classification is named: bacillus subtilis Bacillus subtilis; the preservation units are as follows: china general microbiological culture Collection center (China Committee for culture Collection); the preservation address is: the institute of microorganisms of national academy of sciences of China, national institute of sciences, no. 1, no. 3, north Chen West Lu, the Korean region of Beijing; the preservation date is: 11/6/2020.
Example 3
The embodiment provides a composite microbial agent, which comprises the following components:
the strains used therein were the same as in examples 1 and 2.
Example 4
The embodiment provides a composite microbial agent, which comprises the following components:
the strains used therein were the same as in examples 1 and 2.
Example 5
The embodiment provides a production method of a aspergillus tubingensis agent, which specifically comprises the following steps:
1. slant culture: inoculating Aspergillus tubingensis ZN210 to solid culture medium under aseptic condition, and culturing Aspergillus tubingensis ZN210 at 15deg.C for 6 days;
2. primary seed culture: inoculating the strains cultured in the step 1 into a liquid culture medium respectively under aseptic conditions, and performing shake culture for 6 days at 15 ℃ under 200r/min to obtain first-stage seeds, wherein the optical density OD of the strain suspension of the aspergillus tubingensis ZN210 is 600 Values all reached 3.5;
3. secondary seed culture: according to the inoculation amount of 5% of the volume ratio of the liquid culture medium, inoculating the first-stage seeds into a 100L fermentation tank respectively, wherein the total volume of culture solution in the fermentation tank is 60L, the stirring speed of aspergillus tubingensis ZN210 is 200r/min under the condition of 15 ℃, and the ventilation amount is 1:0.8, culturing for 6 days to obtain the secondary seeds.
4. And (3) three-stage fermentation culture: inoculating the second-level seeds into a fermentation tank with the volume ratio of liquid culture medium being 20%, and performing high-density fermentation culture to obtain the microbial inoculum, wherein the total volume of the culture medium in the fermentation tank is 600L.
Wherein the medium used in steps 1, 2 and 3 for Aspergillus tubingensis ZN210 is PDA medium.
Wherein, the formula of the culture medium used in the step 4 comprises the following components in percentage by mass: 5% of molasses, 8% of peptone and the balance of water;
the high-density fermentation culture adopts a fed-batch culture mode, wherein the fed-batch carbon source is as follows: molasses and glycerol; the nitrogen source is: corn flour and peptone.
The fermentation culture process comprises the following steps: a. aerobic culture stage: during the initial 0-24 hours, ventilation is carried out at intervals, and the fermentation is kept under aerobic condition, and the ventilation rate is 1:1. b. the dissolved oxygen of the fermentation is regulated and controlled to be 10 percent, the stirring rotating speed is 200r/min, the stirring interval time is 3 hours, the stirring is 3 minutes, and the temperature is 30 ℃.
Example 6
The embodiment verifies the antagonism effect of the aspergillus tubingensis microbial inoculum prepared in the embodiment 5 of the embodiment on cucumber fusarium wilt, and the specific flow is as follows:
sterilizing natural soil for later use according to the following steps of 1:60 mass ratio of 10 dilution concentration to the soil 7 cfu/mL of the Aspergillus tubingensis microbial inoculum prepared in example 5 (the microbial inoculum dosage is shown in example 1), with clear water added as a control, was mixed uniformly, and then was sub-packaged in plastic boxes (length×width×height=20 cm×15cm×10 cm) and kept at a proper humidity, and each microbial inoculum was treated to sow 5 boxes and divided into two groups. The cucumber seeds subjected to germination acceleration are sown with 15 seeds. Expanding and culturing pathogenic bacteria of cucumber fusarium wilt in PDA culture solution for 96 hr to obtain the product with concentration of 10 6 cfu/mL spore suspension, and when crop seedlings grow to a 3-leaf stage, adopting a root dipping method: sowing the sterilized and germinated seeds into 50-hole tray filled with sterilized perlite, pulling out seedling in cotyledon flattening stage, cleaning root with water, and standing at 1×10 6 The inoculation solution with colony count cfu/mL is immersed for 5S, then planted in a plastic nutrition pot (6.5 cm multiplied by 6.5 cm) filled with sterilized nutrition soil, cultured at 25 ℃, and then inoculated with pathogenic bacteria. After the disease is controlled and stabilized, observing and recording the disease condition of the crops in each treatment according to the diseaseAnd calculating the disease index and the disease prevention effect according to the hazard severity grading standard in the formula 1 and the formula 2.
Cucumber fusarium wilt severity grading standard:
level 0: the plants are healthy and asymptomatic;
stage 1: slight symptoms appear on the stems and leaves;
2 stages: the plants are slightly withered, necrotic spots appear on stems, and leaves are yellow;
3 stages: the plant is moderately withered in coke, and the leaf drops down to the cucumber;
4 stages: plants severely withered and lodged to die.
The results are shown in Table 1.
Table 1 control effect of aspergillus tubingensis inoculant on cucumber fusarium wilt
The results show that the control effect of the aspergillus tubingensis agent provided in the embodiment 1 on cucumber fusarium wilt is good, and the control effect is obviously better than that of the control.
The invention also carries out a similar experimental flow aiming at trichoderma polyspora ZN105 and bacillus subtilis ZR-2, and results show that compared with a control group, the two microorganisms have the advantages of insignificant improvement of control effect, only less than 40% of improvement, and poor capability of controlling cucumber fusarium wilt.
Example 7
The present example provides a preparation process of the composite microbial agent as shown in examples 2 to 4, which comprises the following steps:
1. slant culture: inoculating original strains of bacillus subtilis ZR-2, trichoderma reesei ZN105 and aspergillus tubingensis ZN210 on a solid culture medium under the aseptic condition, and culturing the trichoderma reesei ZN105 and aspergillus tubingensis ZN210 at the temperature of 30 ℃ for 5 days and culturing the bacillus subtilis ZR-2 at the temperature of 30 ℃ for 2 days;
2. primary seed culture: inoculating the strains cultured in the step 1 into liquid culture medium under aseptic condition, respectively culturing bacillus subtilis ZR-2, trichoderma reesei ZN105 and Aspergillus tubingensis ZN210 at 30deg.C for 3 days in 150r/min shaking table to obtain first-stage seeds, and ending culturing to obtain optical density OD of each strain suspension 600 Values all reached 4.0;
3. secondary seed culture: according to the inoculation amount of 10% of the volume ratio of the liquid culture medium, inoculating the first-stage seeds into a 100L fermentation tank respectively, wherein the total volume of culture solution in the fermentation tank is 60L, the stirring speed is 150r/min under the condition of 30 ℃ and the ventilation rate is 1:1, culturing for 3 days, and culturing bacillus subtilis ZR-2 at 30 ℃ for 2 days to obtain secondary seeds;
4. and (3) mixed fermentation culture: inoculating the second-level seeds into a fermentation tank with the volume ratio of the liquid culture medium being 10-15%, and performing high-density fermentation culture to obtain the microbial inoculum, wherein the total volume of the culture medium in the fermentation tank is 3 tons.
Example 8
The embodiment provides a production method of a composite microbial agent, which specifically comprises the following steps:
1. slant culture: respectively inoculating original strains of bacillus subtilis ZR-2, trichoderma reesei ZN105 and aspergillus tubingensis ZN210 on a solid culture medium under the aseptic condition, and culturing the trichoderma reesei ZN105 and aspergillus tubingensis ZN210 at 15 ℃ for 6 days;
2. primary seed culture: inoculating the strains cultured in the step 1 into a liquid culture medium under aseptic conditions, respectively culturing bacillus subtilis ZR-2, trichoderma reesei ZN105 and Aspergillus tubingensis ZN210 at 15deg.C for 6 days by using a shaking table at 200r/min to obtain first-stage seeds, and ending culturing to obtain optical density OD of each bacterial suspension of bacillus subtilis ZR-2, trichoderma reesei ZN105 and Aspergillus tubingensis ZN210 600 Values all reached 3.0;
3. secondary seed culture: according to the inoculation amount of 5% of the volume ratio of the liquid culture medium, inoculating the first-stage seeds into a 100L fermentation tank respectively, wherein the total volume of culture solution in the fermentation tank is 60L, and the stirring speed is 200r/min and the ventilation amount is 1 under the condition of 15 ℃ of bacillus subtilis ZR-2, trichoderma multiflorum ZN105 and aspergillus tubingensis ZN 210: 0.8, culturing for 6 days to obtain the secondary seeds.
4. And (3) mixed fermentation culture: inoculating the second-level seeds into a fermentation tank with the volume ratio of liquid culture medium being 20%, and performing high-density fermentation culture to obtain the microbial inoculum, wherein the total volume of the culture medium in the fermentation tank is 600L.
Wherein, the culture mediums used in the steps 1, 2 and 3, namely the bacillus subtilis ZR-2, the trichoderma reesei ZN105 and the aspergillus tubingensis ZN210 are PDA culture mediums.
Wherein, the formula of the culture medium used in the step 4 comprises the following components in percentage by mass: 5% of molasses, 8% of peptone and the balance of water;
the high-density fermentation culture adopts a fed-batch culture mode, wherein the fed-batch carbon source is as follows: molasses and glycerol; the nitrogen source is: corn flour and peptone.
The fermentation culture process comprises the following steps: a. aerobic culture stage: during the initial 0-24 hours, ventilation is carried out at intervals, and the fermentation is kept under aerobic condition, and the ventilation rate is 1:1. b. the dissolved oxygen of the fermentation is regulated and controlled to be 10 percent, the stirring rotating speed is 200r/min, the stirring interval time is 3 hours, the stirring is 3 minutes, and the temperature is 30 ℃.
Example 9
The embodiment verifies the antagonism effect of the composite microbial agent prepared by the embodiment on cucumber fusarium wilt, and the specific flow is as follows:
sterilizing natural soil for later use, and adding 10% diluted concentration into the soil according to the mass ratio of 1:60 7 cfu/mL of the compound microbial agent prepared in the example 7 (the microbial agent dosage is shown in the example 3), adding clear water as a control, uniformly mixing, subpackaging in plastic boxes (length, width, height=20 cm, 15cm, 10 cm) and keeping proper humidity, and sowing 5 boxes by each microbial agent treatment and division into two groups. The cucumber seeds subjected to germination acceleration are sown with 15 seeds. Expanding and culturing pathogenic bacteria of cucumber fusarium wilt in PDA culture solution for 96 hr to obtain the product with concentration of 10 6 cfu/mL spore suspension, and when crop seedlings grow to a 3-leaf stage, adopting a root dipping method: sowing the seeds after disinfection and germination acceleration onExtracting seedling in cotyledon flattening stage in 50-hole tray filled with sterilized perlite, cleaning root with water, and culturing at 1×10 6 The inoculation solution with colony count cfu/mL is immersed for 5S, then planted in a plastic nutrition pot (6.5 cm multiplied by 6.5 cm) filled with sterilized nutrition soil, cultured at 25 ℃, and then inoculated with pathogenic bacteria. After the disease is controlled and stabilized, observing and recording the disease condition of the crops in each treatment, and calculating the disease index and the disease prevention effect according to the disease severity grading standard and the formulas 1 and 2.
Cucumber fusarium wilt severity grading standard:
level 0: the plants are healthy and asymptomatic;
stage 1: slight symptoms appear on the stems and leaves;
2 stages: the plants are slightly withered, necrotic spots appear on stems, and leaves are yellow;
3 stages: the plant is moderately withered in coke, and the leaf drops down to the cucumber;
4 stages: plants severely withered and lodged to die.
The results are shown in Table 2.
Table 2 control effect of composite microbial inoculum on cucumber fusarium wilt
The results show that the composite microbial agent provided in the embodiment 3 has obviously better control effect on cucumber fusarium wilt than that of the control.
Example 10
In this example, the influence of the composite microbial agent prepared in the above example on secondary composting is verified, and in this example, an experiment is performed by using the composite microbial agent prepared in example 8 (the microbial agent dosage is example 4), and the specific flow is as follows:
cow dung, chicken manure and mushroom residue composting: the composting process is carried out in the field at the organic fertilizer production factory of the sinus store of Beijing Fangshan. The compost proportioning (mass ratio) is as follows: cow dung: chicken manure: mushroom residue=6:5:5, and primary fermentation adopts an aerobic tank composting mode.
And (3) performing secondary fermentation by using compost fermented for about 28 days, wherein a 100 liter small-sized box-type fermentation device is adopted in the secondary fermentation process to simulate a static aeration aging mode in actual production, and the stacking height is about 0.6m. Each process was repeated three times, periodically turning the stack, with a set period of 12d.
The secondary fermentation is carried out in a small box type compost fermenter (heat preservation foam material), and the optimal composite microbial agent is inoculated in the secondary fermentation process, so that the compost temperature, moisture and air detection equipment are measured, and the temperature and moisture change in the secondary fermentation process is tracked.
As shown in fig. 1 and 2, the temperature of the stack inoculated with the composite microbial agent tends to rise, the stack is promoted to be further fermented, the moisture of the stack ventilated by adding the microbial agent is reduced most rapidly, and the ventilation design provides a certain theoretical basis for the final production of the commercial organic fertilizer and the design of the technological parameter of rapid moisture reduction.
The above results demonstrate that the addition of aspergillus tubingensis and the composite microbial agent is beneficial to reducing the production cost of the organic fertilizer, wherein the disease-preventing microorganisms can improve the functionality of the organic fertilizer, and the microbial agent has an obvious effect of improving the quality of the biological organic fertilizer.
While the invention has been described in detail in the foregoing general description and with reference to specific embodiments thereof, it will be apparent to one skilled in the art that modifications and improvements can be made thereto. Accordingly, such modifications or improvements may be made without departing from the spirit of the invention and are intended to be within the scope of the invention as claimed.
Claims (10)
1. Aspergillus tubingensis (S.tubingensis)Aspergillus tubingensis) ZN210, characterized in that the aspergillus tubingensis isAspergillus tubingensis) The preservation number of ZN210 is CGMCC No.22412.
2. A composite microbial agent is characterized by comprising the aspergillus tubingensis of claim 1Aspergillus tubingensis）ZN210。
3. The composite microbial agent of claim 2, further comprising bacillus subtilis and trichoderma polyspora.
4. The composite microbial agent of claim 3, wherein the bacillus subtilis is bacillus subtilis @Bacillus subtilis) ZR-2 with a preservation number of CGMCC NO.21124;
and/or the trichoderma reesei is trichoderma reesei @Trichoderma polysporum) ZN105 has a preservation number of CGMCC No.22411.
5. The composite microbial agent of claim 4, wherein the agent comprises:
aspergillus tubingensis (L.) KuntzeAspergillus tubingensis）ZN210 1.0×10 4 -2.0×10 5 cfu/mL, bacillus subtilisBacillus subtilis） ZR-2 1.0×10 9 -8.0×10 9 cfu/mL and Trichoderma reeseiTrichoderma polysporum）ZN105 1.0×10 4 -6.0×10 5 cfu/mL。
6. The compound microbial agent of claim 5, which is characterized by comprising aspergillus tubingensisAspergillus tubingensis）ZN210 1.5×10 5 -2.0×10 5 cfu/mL, bacillus subtilisBacillus subtilis） ZR-2 5.0×10 9 -6.0×10 9 cfu/mL and Trichoderma reeseiTrichoderma polysporum）ZN105 2.0×10 5 -4.0×10 5 cfu/mL。
7. The method for preparing the composite microbial agent as claimed in claim 5 or 6, which is characterized by comprising the following steps:
the aspergillus tubingensis is treated withAspergillus tubingensis) ZN210 and bacillus subtilisBacillus subtilis) ZR-2 and Trichoderma polysporaTrichoderma polysporum) ZN105 is fermented and cultured to obtain the microbial inoculum.
8. The aspergillus tubingensis strain according to claim 1Aspergillus tubingensis) Use of ZN210, or a composite microbial agent as defined in any one of claims 2 to 6, in secondary composting of organic material.
9. The use according to claim 8, characterized in that the use is:
promote organic matter to decompose.
10. The aspergillus tubingensis strain according to claim 1Aspergillus tubingensis) The use of ZN210, or a composite microbial agent as defined in any one of claims 2 to 6, in the prevention and treatment of cucumber fusarium wilt.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105907680A (en) * | 2016-05-27 | 2016-08-31 | 河北省科学院生物研究所 | Bacillus subtilis J-5 as well as biological agent and application thereof |
| CN106591157A (en) * | 2017-02-23 | 2017-04-26 | 北京市农林科学院 | Aspergillus tubingensis with disease prevention and growth promoting functions as well as preparation and application of aspergillus tubingensis metabolites |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105907680A (en) * | 2016-05-27 | 2016-08-31 | 河北省科学院生物研究所 | Bacillus subtilis J-5 as well as biological agent and application thereof |
| CN106591157A (en) * | 2017-02-23 | 2017-04-26 | 北京市农林科学院 | Aspergillus tubingensis with disease prevention and growth promoting functions as well as preparation and application of aspergillus tubingensis metabolites |
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