CN114451403A - Operating device for vitrification treatment of biological material - Google Patents
Operating device for vitrification treatment of biological material Download PDFInfo
- Publication number
- CN114451403A CN114451403A CN202210289352.8A CN202210289352A CN114451403A CN 114451403 A CN114451403 A CN 114451403A CN 202210289352 A CN202210289352 A CN 202210289352A CN 114451403 A CN114451403 A CN 114451403A
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- China
- Prior art keywords
- channel
- cavity
- vitrification
- biological material
- containing cavity
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000012620 biological material Substances 0.000 title claims abstract description 61
- 238000004017 vitrification Methods 0.000 title claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 41
- 230000008014 freezing Effects 0.000 abstract description 36
- 238000007710 freezing Methods 0.000 abstract description 36
- 206010066054 Dysmorphism Diseases 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005213 imbibition Methods 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 239000002577 cryoprotective agent Substances 0.000 description 1
- 230000000959 cryoprotective effect Effects 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
- A01N1/0268—Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen
Abstract
The invention relates to an operating device for biological material vitrification treatment, which comprises a body and an opening valve, wherein the body is provided with a first containing cavity and a second containing cavity, the first containing cavity is communicated with the second containing cavity through a first channel, the first channel only allows liquid to pass through, a second channel communicated with the second containing cavity and the outside is arranged in the body, the opening valve is detachably arranged in the second channel, and the opening valve can seal the second channel. The operation device can stably carry out freezing pretreatment on the biological material and ensure the consistency of the freezing pretreatment operation.
Description
Technical Field
The invention relates to the technical field of biological material treatment, in particular to an operating device for biological material vitrification treatment.
Background
At present, the vitrification pretreatment of the biological material is usually completed manually by operators, and the operation habits and operation skills of different operators are different, so that the quality of the vitrification pretreatment of the biological material cannot be ensured. Some present automated designs can be through setting up an operation groove, the operation inslot sets up and is used for keeping off the biomaterial and prevent the dysmorphism groove that runs off, when freezing the preliminary treatment, the biomaterial places in the dysmorphism inslot, then to pour into operation liquid into the operation inslot, treat freezing the preliminary treatment back, will contain biomaterial dysmorphism groove sealed back, directly drop into in the liquid nitrogen, this kind of method is not suitable for present biomaterial freezing year pole, and this kind of mode biomaterial blocks very easily in the dysmorphism inslot, can't be fast and accurate completion to biomaterial take out and transfer to on carrying the pole.
Disclosure of Invention
In view of the above, it is necessary to provide an operation device for vitrification processing of a biological material, which can stably freeze-pretreat the biological material and ensure consistency of the operation of the freeze-pretreatment, in order to solve the problem that the effect of the freeze-pretreatment of the biological material cannot be ensured.
An operating device for vitrification processing of biological materials, comprising: the liquid filling device comprises a body and an opening valve, wherein the body is provided with a first containing cavity and a second containing cavity, the first containing cavity is communicated with the second containing cavity through a first channel, the first channel only allows liquid to pass through, a second channel communicated with the second containing cavity and the outside is arranged in the body, the opening valve is detachably arranged in the second channel, and the opening valve can seal the second channel.
Above-mentioned operating means for biomaterial vitrification treatment can install the opening valve in the second passageway to with second passageway internal seal, then add the biomaterial to the second appearance intracavity by the operator, add right amount of freezing protection liquid to first appearance intracavity, and because the setting of first passageway, the freezing protection liquid in first appearance intracavity can circulate to the second appearance intracavity, freeze the preliminary treatment to the biomaterial, after reaching the processing time of reservation, with freezing protection liquid suction, accomplished the freezing preliminary treatment to the biomaterial this moment. Next, the open valve is removed and the frozen pretreated biological material is then transferred through a second channel to a freezing carrier rod for storage. This operating means can be fast, stably to the freezing preliminary treatment operation of biomaterial, and can guarantee freezing preliminary treatment operation's uniformity, does not receive the influence of operator experience and skill, and the biomaterial after freezing can obtain quick transfer and save, has guaranteed the quality of the biomaterial who obtains effectively, has avoided manual processing to shift the risk that biomaterial caused the harm. The biological material may be a biological germ cell.
In one embodiment, the bottom of the first channel extends obliquely. The cryoprotectant solution may be facilitated to flow between the first and second volumes.
In one embodiment, the height of the bottom end of the first passage connected to the first cavity is lower than the height of the bottom end of the first passage connected to the second cavity. Can guarantee that freezing protection liquid holds the freezing preliminary treatment back of biomaterial in the intracavity to the second, fast reflux to first appearance intracavity, and then makes freezing protection liquid can be followed first appearance intracavity and by quick suction.
In one embodiment, a first matching portion is arranged at the end of the first accommodating cavity, a second matching portion is arranged at the end of the second accommodating cavity, and the first matching portion and the second matching portion are staggered to form the first channel. The arrangement mode can conveniently and effectively realize the formation of the first channel, and the technical problem that the first channel is difficult to process in order to meet the requirement of small size is improved.
In one embodiment, the first channel is a capillary channel. The freezing protection liquid can conveniently and effectively flow into the second containing cavity from the first containing cavity to carry out freezing pretreatment on the biological material, and quickly flow back into the first containing cavity after the freezing pretreatment.
In one embodiment, the first matching portion and the second matching portion are spaced apart from each other in a direction perpendicular to a liquid flowing direction, and an end surface of the first matching portion facing the second cavity is flush with an end surface of the second matching portion facing the first cavity or staggered in the liquid flowing direction.
In one embodiment, an expansion portion is arranged in the first cavity, the expansion portion is communicated with the first channel, and the width of the expansion portion is gradually increased from one side connected with the first channel to the middle of the first cavity. The setting of expansion portion can the compatible liquid head and the imbibition head of matcing not equidimension model, annotates the liquid head and can annotate liquid to first appearance intracavity.
In one embodiment, the first cavity and the second cavity are disposed at the top of the body. In one embodiment, the first cavity is tapered, square or circular.
In one embodiment, the inner bottom surface of the second cavity extends obliquely, and the height of the inner bottom surface of the second cavity gradually decreases from the side far away from the first passage to the first cavity.
In one embodiment, the second passage is a circular passage, and the opening valve has a cylindrical end capable of being inserted into the second passage and sealing the second passage.
In one embodiment, a flange is protruded from the outer periphery of the cylindrical end, the flange is annular, and the flange is tightly matched with the second channel.
In one embodiment, the body includes a first body, a second body and a third body, the first body is connected to the top ends of the second body and the third body, respectively, and the bottom end of the first body is spaced from the bottom ends of the second body and the third body.
Drawings
FIG. 1 is a schematic structural diagram of an embodiment of an operating device according to the present invention;
FIG. 2 is a schematic structural diagram of another embodiment of an operating device according to the present invention;
FIG. 3 is a schematic view of the top of the manipulator of FIG. 2;
FIG. 4 is an enlarged view of a portion A of FIG. 3;
FIG. 5 is a schematic structural diagram of another embodiment of an operating device according to the present invention;
FIG. 6 is a schematic view of a portion of the operating device shown in FIG. 5;
fig. 7 is a schematic structural diagram of an embodiment of the opening valve of the present invention.
In the drawings, the components represented by the respective reference numerals are listed below:
100. an operating device for vitrification treatment of biological material; 1. a body; 11. a first cavity; 111. an expansion section; 112. a first mating portion; 113. a first end face; 12. a second cavity; 121. a second mating portion; 122. a second end face; 13. a first channel; 14. a second channel; 17. a first body; 18. a second body; 19. a third body; 2. opening the valve; 21. a cylindrical end; 22. a flange; .
Detailed Description
In order to make the aforementioned and other objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. It is apparent that the specific details set forth in the following description are merely exemplary of the invention, which can be practiced in many other embodiments that depart from the specific details disclosed herein. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without any inventive step, are within the scope of the present invention.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only and do not represent the only embodiments.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
Referring to fig. 1 and 2, in one embodiment, an apparatus 100 for vitrification processing of a biological material includes: a body 1 and an opening valve 2. The body 1 is provided with a first cavity 11 and a second cavity 12, the first cavity 11 is communicated with the second cavity 12 through a first channel 13, and the first channel 13 only allows liquid to pass through. A second channel 14 communicated with the second cavity 12 and the outside is arranged in the body 1, the opening valve 2 is detachably arranged in the second channel 14, and the opening valve 2 can seal the second channel 14.
This operating means 100 for biomaterial vitrification treatment can install the opening valve 2 in the second passageway 14 to with second passageway 14 internal seal, then add the biomaterial to the second by the operator and hold the intracavity 12, add right amount of freezing protection liquid to the first appearance intracavity 11, and because the setting of first passageway 13, the freezing protection liquid in the first appearance intracavity 11 can circulate to the second and hold intracavity 12, freeze the preliminary treatment to the biomaterial, after reaching the processing time of reservation, with freezing protection liquid suction, the freezing preliminary treatment to the biomaterial has been accomplished at this moment. Next, the open valve 2 needs to be removed and the frozen pre-treated biological material is flow transferred to the freezing carrier bar for storage through the second channel 14. The operation device can quickly and stably carry out freezing pretreatment operation on the biological material, can ensure the consistency of the freezing pretreatment operation, is not influenced by the experience and skill of an operator, can quickly transfer and store the pretreated biological material, and effectively ensures the quality of the obtained biological material. Not only can avoid the risk that the biological material causes damage or loses because of the operation in freezing pretreatment process, but also can improve the reliability and the convenience of handling, guarantee the normal clear of follow-up freezing pretreatment.
In one embodiment, the first and second volumes 11 and 12 are located on the top of the body 1 to facilitate the freezing pretreatment operation of the biological material.
The bottom of the first channel 13 extends obliquely in view of the ease and fluency of the flow of the cryoprotective liquid between the first chamber 11 and the second chamber 12.
Further, the height of the bottom end of the first passage 13 connected with the first cavity 11 is lower than the height of the bottom end of the first passage 13 connected with the second cavity 12. After the freezing pretreatment of the biological material in the second containing cavity 12 by the freezing protection liquid, the biological material rapidly flows back to the first containing cavity 11, and then the freezing protection liquid can be rapidly sucked out from the first containing cavity 11.
The first channel 13 may be a capillary channel, which can conveniently and effectively realize that the freezing protection liquid flows into the second cavity 12 from the first cavity 11 to perform freezing pretreatment on the biological material, and quickly flows back into the first cavity 11 after the freezing pretreatment.
Referring to fig. 5 and 6, in an embodiment, a first matching portion 112 is disposed at an end of the first cavity 11, a second matching portion 121 is disposed at an end of the second cavity 12, and the first matching portion 112 and the second matching portion 121 are staggered to form the first channel 13. The arrangement mode can conveniently and effectively realize the formation of the first channel 13, and improves the technical problem that the first channel 13 is difficult to machine in order to meet the requirement of smaller size. It is possible to achieve a gap of 0.08mm for the first channel 13 directly by injection moulding, while it is otherwise difficult to achieve such a gap of extremely small size. On the basis of the structure, the liquid can be injected into the first cavity 11, the liquid actively flows into the second cavity 12 to freeze and pretreat the biological material, after the liquid is treated, the liquid is absorbed in the first cavity 11, the liquid in the second cavity 12 flows into the first cavity 11 through the first channel 13 and is absorbed away, and in the operation process, the phenomenon that the biological material is lost or the biological material flows into the first cavity 11 through the first channel 13 can not occur completely.
Referring to fig. 6, in an embodiment of the first channel 13, the first matching portion 112 and the second matching portion 121 are spaced apart from each other in a direction perpendicular to a liquid flowing direction, and the first end surface 113 of the first matching portion 112 facing the second cavity 12 is flush with the second end surface 122 of the second matching portion 121 facing the first cavity 11 or staggered in the liquid flowing direction.
Referring to fig. 3 and 4, in other embodiments, an expansion portion 111 is disposed in the first cavity 11, the expansion portion 111 is communicated with the inside of the first cavity 11 and the first channel 13, and a width of the expansion portion 111 gradually increases from a side connected with the first channel 13 to a middle of the first cavity 11. The setting of expansion portion 111 can the compatible liquid head and the imbibition head of matcing of not equidimension model, annotates the liquid head and can annotate liquid to first appearance intracavity 11.
Referring to fig. 1, in another embodiment of the body 1, the body 1 includes a first main body 17, a second main body 18 and a third main body 19, the first main body 17 is connected to the top ends of the second main body 18 and the third main body 19, respectively, and the bottom end of the first main body 17 is spaced from the bottom ends of the second main body 18 and the third main body 19.
The shape of the first receptacle 11 may be conical, square or circular.
In an embodiment of the second cavity 12, the inner bottom surface of the second cavity 12 extends obliquely, and the height of the inner bottom surface of the second cavity 12 gradually decreases from a side far away from the first passage 13 to the first cavity 11. It should be noted that the bottom surface of the inner side of the second cavity 12, which is communicated with the first channel 13, is the lowest point of the residual liquid after the freezing protection liquid is sucked, and the residual liquid amounts with different requirements can be obtained through the structure of the lowest point.
Referring to fig. 1, in other embodiments, the second passage 14 is a circular passage, and the opening valve 2 has a cylindrical end 21, and the cylindrical end 21 can be inserted into the second passage 14. Further, a flange 22 is protruded from the outer periphery of the cylindrical end 21, the flange 22 is annular, and the flange 22 is tightly fitted with the second channel 14, so that the opening valve 2 can rapidly seal the second channel 14, and the sealing effect is good.
Referring to fig. 7, it can be understood that the second channel 14 is exposed from the bottom of the body 1 and may be tapered, which facilitates the flow of the biological material from the second channel 14 and the rapid flow of the biological material onto the carrier rod without damaging the biological material.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for those skilled in the art, various changes, substitutions and alterations can be made without departing from the spirit and scope of the invention. Therefore, the protection scope of the present invention should be subject to the claims.
Claims (10)
1. An operating device for vitrification processing of biological material, comprising: the liquid filling device comprises a body and an opening valve, wherein the body is provided with a first containing cavity and a second containing cavity, the first containing cavity is communicated with the second containing cavity through a first channel, the first channel only allows liquid to pass through, a second channel communicated with the second containing cavity and the outside is arranged in the body, the opening valve is detachably arranged in the second channel, and the opening valve can seal the second channel.
2. The handling device for vitrification of biological material according to claim 1, wherein a bottom of the first channel is extended obliquely.
3. The manipulating device for the vitrification of biological materials according to claim 2, wherein a bottom end height of the first channel connected to the first cavity is lower than a bottom end height of the first channel connected to the second cavity.
4. The handling device for the vitrification of a biomaterial according to claim 1, wherein a first engaging portion is provided at an end of the first cavity, a second engaging portion is provided at an end of the second cavity, and the first engaging portion and the second engaging portion are staggered to form the first channel.
5. The manipulating device for the vitrification processing of a biomaterial according to claim 4, wherein the first fitting portion and the second fitting portion are spaced apart in a direction perpendicular to a liquid flowing direction, and an end surface of the first fitting portion facing the second cavity is flush with an end surface of the second fitting portion facing the first cavity or staggered in a liquid flowing direction.
6. The operating device for the vitrification of biological materials according to claim 1, wherein an expansion portion is provided in the first chamber, the expansion portion is communicated with the first channel, and a width of the expansion portion is gradually increased from a side connected to the first channel to a middle of the first chamber.
7. The handling device for the vitrification of biological materials according to claim 1, wherein the inner bottom surface of the second cavity extends obliquely, and the height of the inner bottom surface of the second cavity becomes gradually lower from a side away from the first channel to the first cavity.
8. The handling device for vitrification of biological material according to claim 1 wherein the second channel is a circular channel and the opening valve has a cylindrical end that is insertable into the second channel and seals the second channel.
9. The handling device for vitrification of biological material according to claim 8 wherein the cylindrical end has a flange projecting from its outer periphery, the flange having an annular shape and being in close fit with the second channel.
10. The handling device for vitrification of biological material according to claim 1 wherein the body includes a first body, a second body and a third body, the first body being connected to top ends of the second body and the third body, respectively, and a bottom end of the first body being spaced from bottom ends of the second body and the third body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210289352.8A CN114451403A (en) | 2022-03-22 | 2022-03-22 | Operating device for vitrification treatment of biological material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210289352.8A CN114451403A (en) | 2022-03-22 | 2022-03-22 | Operating device for vitrification treatment of biological material |
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| Publication Number | Publication Date |
|---|---|
| CN114451403A true CN114451403A (en) | 2022-05-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210289352.8A Pending CN114451403A (en) | 2022-03-22 | 2022-03-22 | Operating device for vitrification treatment of biological material |
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| Country | Link |
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| CN (1) | CN114451403A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111700064A (en) * | 2020-07-22 | 2020-09-25 | 明日加加科技有限公司 | Cell freezing auxiliary device |
| CN214339670U (en) * | 2021-01-15 | 2021-10-08 | 香港物流机械人研究中心有限公司 | Biological material processing apparatus |
| CN216931626U (en) * | 2022-03-22 | 2022-07-12 | 香港中文大学深港创新研究院(福田) | Operating device for vitrification treatment of biological material |
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2022
- 2022-03-22 CN CN202210289352.8A patent/CN114451403A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111700064A (en) * | 2020-07-22 | 2020-09-25 | 明日加加科技有限公司 | Cell freezing auxiliary device |
| CN214339670U (en) * | 2021-01-15 | 2021-10-08 | 香港物流机械人研究中心有限公司 | Biological material processing apparatus |
| CN216931626U (en) * | 2022-03-22 | 2022-07-12 | 香港中文大学深港创新研究院(福田) | Operating device for vitrification treatment of biological material |
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