CN219168456U

CN219168456U - Quick sample adding device

Info

Publication number: CN219168456U
Application number: CN202223543172.2U
Authority: CN
Inventors: 宋一之; 胡慧杰; 张镇川; 王卫东
Original assignee: Jinan Hi Tech Achievements Transformation Brokerage Co ltd; Suzhou Institute of Biomedical Engineering and Technology of CAS
Current assignee: Jinan Hi Tech Achievements Transformation Brokerage Co ltd; Suzhou Institute of Biomedical Engineering and Technology of CAS
Priority date: 2022-12-28
Filing date: 2022-12-28
Publication date: 2023-06-13
Anticipated expiration: 2032-12-28

Abstract

The utility model discloses a rapid sample adding device, which belongs to the field of medical equipment and comprises a sample plate and a containing plate, wherein the sample plate is provided with a sample containing part and a plurality of liquid guiding needles positioned in the sample containing part, the containing plate comprises a main body, a first sealing film and a second sealing film, the main body is provided with a plurality of containing holes and sample injection flow passages, the sample injection flow passages are communicated with the containing holes, the first sealing film seals the end parts of the containing holes, the second sealing film seals the sample injection flow passages, negative pressure is formed in the containing holes and the sample injection flow passages communicated with the containing holes, when the rapid sample adding device is used, the containing plate is arranged on the sample plate, and the liquid guiding needles pierce the second sealing film to enable liquid samples in the sample containing part to flow into each containing hole along the sample injection flow passages respectively.

Description

Quick sample adding device

Technical Field

The utility model relates to the field of medical equipment, in particular to a rapid sample adding device.

Background

In the biomedical field, there is often a scenario in which a sample needs to be added to each well in a multi-well plate, while communication and mutual contamination between the wells are not allowed. In the prior art, a disposable pipette gun is adopted, and the disposable pipette tips of the pipette gun need to be replaced between sample adding holes, so that time and labor are wasted, and consumption of the disposable pipette tips is increased.

Disclosure of Invention

In order to overcome the defects of the prior art, one of the purposes of the utility model is to provide a rapid sample adding device which can sample a plurality of containing holes at one time and avoid the mixing pollution of solutions in the containing holes.

One of the purposes of the utility model is realized by adopting the following technical scheme:

the utility model provides a quick sample application device, includes sample board and accommodation plate, the sample board is equipped with sample acceptors and a plurality of being located the drawing liquid needle of sample acceptors, the accommodation plate includes main part, first sealing membrane and second sealing membrane, the main part is equipped with a plurality of accommodation holes and advances the appearance runner, advance the appearance runner with it communicates to hold the hole, first sealing membrane seals hold hole tip, the second sealing membrane seals advance the appearance runner, make hold the hole and with form the negative pressure in the advance appearance runner that holds the hole intercommunication, when quick sample application device used, the accommodation plate install in the sample board, draw liquid needle puncture second sealing membrane makes liquid sample in the sample acceptors follows advance appearance runner inflow each respectively hold the hole.

Further, the number of the sample injection flow passages is the same as that of the containing holes, each sample injection flow passage extends from the bottom of the outer wall of the containing hole to the position close to the top, and the sample injection flow passages are communicated with the inside of the containing hole at the position close to the top of the containing hole.

Further, the inner wall of the accommodating hole is provided with a protrusion and a recess, the protrusion and the recess are correspondingly arranged and folded to form a sample injection flow passage, and the top of the sample injection flow passage is communicated with the accommodating hole.

Further, the accommodating hole is of a hollow structure, the accommodating hole comprises a bottom surface, the sample injection runner extends to the bottom surface and forms a through hole on the bottom surface, and the second sealing film seals the through hole.

Furthermore, the liquid guiding needle is of a hollow structure.

Further, the liquid-guiding needle is provided with a notch, the notch extends along the extending direction of the liquid-guiding needle, and the inside of the liquid-guiding needle is communicated with the sample accommodating part through the notch.

Further, the end part of the liquid guiding needle is conical.

Further, the sample plate is further provided with a sample adding groove, a liquid inlet, an overflow port and a waste liquid groove, the sample adding groove is communicated with the sample accommodating part through the liquid inlet, and the sample accommodating part is communicated with the waste liquid groove through the overflow port.

Furthermore, the bottom of the sample adding tank is obliquely arranged, and the lowest part of the bottom of the sample adding tank is connected with the bottom of the liquid inlet.

Further, the sample accommodating part is provided with a plurality of sample holes, and the plurality of sample holes are communicated through a main flow channel.

Compared with the prior art, the sample plate of the rapid sample adding device is provided with the sample accommodating part and the plurality of liquid guiding needles positioned in the sample accommodating part, the accommodating plate comprises a main body, a first sealing film and a second sealing film, the main body is provided with a plurality of accommodating holes and sample injection flow passages, the sample injection flow passages are communicated with the accommodating holes, the end parts of the accommodating holes are sealed by the first sealing film, the sample injection flow passages are sealed by the second sealing film, negative pressure is formed in the accommodating holes and the sample injection flow passages communicated with the accommodating holes, when the rapid sample adding device is used, the accommodating plate is arranged on the sample plate, and the liquid guiding needles pierce the second sealing film to enable liquid samples in the sample accommodating part to flow into each accommodating hole along the sample injection flow passages respectively.

Drawings

FIG. 1 is a perspective view of a rapid sample addition device of the present utility model;

FIG. 2 is an exploded view of the rapid loading device of FIG. 1;

FIG. 3 is a perspective view of a sample plate of the rapid sample addition device of FIG. 2;

FIG. 4 is a cross-sectional view of the sample plate of FIG. 3;

FIG. 5 is an exploded view of the containment plate of the rapid prototyping apparatus of FIG. 2;

FIG. 6 is a perspective view of the body of the containment plate of FIG. 5;

fig. 7 is an enlarged view at a of the body of fig. 6;

FIG. 8 is another perspective view of the body of FIG. 6;

FIG. 9 is a cross-sectional view of the rapid loading device of FIG. 1;

fig. 10 is an enlarged view of the rapid loading device of fig. 9 at B.

In the figure: 11. a sample plate; 111. a sample adding groove; 112. a liquid inlet; 113. a main flow passage; 114. a sample storage section; 115. an overflow port; 116. a waste liquid tank; 117. a first seal groove; 118. a liquid-guiding needle; 12. a receiving plate; 120. a main body; 1201. a receiving hole; 12010. an inner wall; 12011. a protrusion; 12012. a top end; 12013. a recess; 12014. a bottom surface; 12015. a through hole; 1202. a sample injection flow passage; 1203. a sample adding port; 1204. a first end face; 1205. a second end face; 1206. a second seal groove; 121. a first sealing film; 122. a second sealing film; 13. and (3) sealing rings.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

It will be understood that when an element is referred to as being "fixed to" another element, it can be directly on the other element or be present as another intermediate element through which the element is fixed. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. When an element is referred to as being "disposed on" another element, it can be directly on the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like are used herein for illustrative purposes only.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this utility model belongs. The terminology used herein in the description of the utility model is for the purpose of describing particular embodiments only and is not intended to be limiting of the utility model. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.

As shown in fig. 1 to 10, the rapid sample loading device of the present utility model includes a sample plate 11, a receiving plate 12, and a sealing ring 13.

The sample plate 11 is provided with a sample addition well 111, a liquid inlet 112, a main channel 113, a sample storage portion 114, an overflow port 115, a waste liquid tank 116, a first seal groove 117, and a liquid introduction needle 118.

The loading slot 111 is located at the end of the sample plate 11. In this embodiment, the sample addition groove 111 is in a strip shape, the bottom of the sample addition groove 111 is an inclined plane, and the lowest part of the inclined plane is connected with the liquid inlet 112.

The liquid inlet 112 is located between the sample addition groove 111 and the sample storage part 114. The solution sample enters the sample storage section 114 through the sample addition groove 111 and the liquid inlet 112. In this embodiment, the sample receiving portion 114 is a plurality of cylindrical holes, and the plurality of cylindrical holes are mutually communicated through the main flow channel 113, so that only a small amount of solution sample is required to fill the sample receiving portion 114. In other embodiments, the sample receiving portion 114 may also be a rectangular recess.

An overflow port 115 is provided at the other end of the sample receiving portion 114, and when the amount of the added solution sample is excessive, the solution sample overflows from the overflow port 115 to a waste liquid tank 116, maintaining the uniformity of the height of the sample solution.

The first seal groove 117 is used for mounting a seal ring 13, and the seal ring 13 seals the sample plate 11 from the accommodating plate 12, so that the sample solution in the sample plate 11 is prevented from overflowing through the contact surface.

The number of the liquid introducing needles 118 is plural. Specifically, the number of the liquid-introducing needles 118 is the same as the number of the accommodating holes 1201, so that each liquid-introducing needle 118 can correspond to one accommodating hole 1201. The liquid-guiding needle 118 has a hollow structure, the liquid-guiding needle 118 is provided with a notch, the notch extends along the extending direction (vertical direction) of the liquid-guiding needle 118, and the inside of the liquid-guiding needle 118 is communicated with the sample accommodating portion 114 through the notch. The end of the drainage needle 118 is tapered to facilitate the drainage needle 118 piercing the second sealing membrane 122.

The accommodating plate 12 includes a main body 120, a first sealing film 121, and a second sealing film 122.

The main body 120 is provided with a plurality of accommodating holes 1201, a plurality of sample injection flow passages 1202, a sample injection port 1203, a first end surface 1204, a second end surface 1205, and a second seal groove 1206.

A plurality of receiving holes 1201 are provided on the first end surface 1204, and each receiving hole 1201 extends downward from the first end surface 1204 to have a hollow structure.

The number of the sample introduction flow passages 1202 is the same as the number of the accommodating holes 1201. Each sample injection flow channel 1202 extends from the bottom of the outer wall of the receiving hole 1201 to a position near the top of the receiving hole 1201. The injection flow path 1202 communicates with the inside of the accommodating hole 1201 near the top of the accommodating hole 1201. Specifically, the inner wall 12010 of the accommodating hole 1201 is provided with a protrusion 12011 and a recess 12013, the protrusion 12011 and the recess 12013 are correspondingly arranged and folded to form a sample injection flow channel 1202, and the top 12012 of the sample injection flow channel 1202 is communicated with the accommodating hole 1201. The accommodating hole 1201 includes a bottom surface 12014, the sample introduction flow path 1202 extends to the bottom surface 12014 and forms a through hole 12015 in the bottom surface 12014, and the second sealing film 122 seals the through hole 12015.

The loading port 1203 is located at an end of the receiving plate 12, and the loading port 1203 is used to pour the solution template into the loading slot 111. The first end face 1204 is lower in height than the second end face 1205, facilitating the mounting of the first sealing film 121 against the first end face 1204, achieving a tight seal of the receiving hole 1201. The second seal groove 1206 is used for mounting a seal ring 13, the seal ring 13 sealing the sample plate 11 from the receiving plate 12, avoiding overflow of the sample solution in the sample plate 11 through the contact surface.

In the case of using the rapid sample application device of the present utility model, the sample plate 11 is separated from the holding plate 12 in the initial state.

Each accommodating hole 1201 of the accommodating plate 12 is filled with different kinds and concentrations of medicines, the sample injection flow channel 1202 is the only passage for the sample solution to enter the accommodating hole 1201 from the sample accommodating part 114, the through hole 12015 at the lower end of the sample injection flow channel 1202 is sealed by the second sealing film 122, and the height of the first end surface 1204 is lower than that of the second end surface 1205, so that the first sealing film 121 is conveniently attached to the first end surface 1204, and the tight sealing of the accommodating hole 1201 is realized. The first sealing film 121 and the second sealing film 122 cooperate to keep the inside of the accommodating hole 1201 completely sealed, and the inside of the accommodating hole 1201 is a negative pressure environment with a certain vacuum degree. The suction amount of the sample solution is controlled by controlling the vacuum degree in the accommodating hole 1201, so that the sample solution can be more conveniently and uniformly and accurately transferred.

The sample solution is poured into the sample adding groove 111, flows into the main flow channel 113 through the liquid inlet 112, is uniformly distributed to the sample accommodating part 114 through the main flow channel 113, is at least higher than the through hole 12015 of the sample injection flow channel 1202, and flows into the waste liquid groove 116 through the overflow port 115.

The accommodating plate 12 is arranged on the sample plate 11, during the installation process, the accommodating plate 12 is pressed downwards to the sample plate 11, the sample plate 11 is sealed with the accommodating plate 12 by the sealing ring 13, and the sample solution in the sample plate 11 is prevented from overflowing through the contact surface. Simultaneously, the liquid-introducing needle 118 pierces the second sealing film 122, and under the negative pressure of the accommodating hole 1201, the sample solution enters the accommodating hole 1201 along the liquid-introducing needle 118 through the sample-introducing flow passage 1202 to be mixed with the dry powder of the medicine to form a mixed solution. The suction amount of the sample solution is controlled by controlling the vacuum degree in the accommodating hole 1201, so that the sample solution can be more conveniently and uniformly and accurately transferred.

In this application, through the vacuum in the control accommodation hole 1201, realize that sample solution shifts to accommodation plate 12 from sample board 11 accurately, saved the loaded down with trivial details process that traditional composite board single hole was pipetted one by one, avoided the waste that the disposable pipette tip of change caused simultaneously.

The foregoing examples illustrate only a few embodiments of the utility model, which are described in detail and are not to be construed as limiting the scope of the utility model. It should be noted that, for those skilled in the art, it is possible to make several modifications and improvements without departing from the concept of the present utility model, which are equivalent to the above embodiments according to the essential technology of the present utility model, and these are all included in the protection scope of the present utility model.

Claims

1. A quick application of sample device which characterized in that: including sample board and accommodation plate, the sample board is equipped with sample acceptors and a plurality of being located the drainage needle of sample acceptors, the accommodation plate includes main part, first sealing membrane and second sealing membrane, the main part is equipped with a plurality of accommodation holes and advances the appearance runner, advance the appearance runner with it communicates to hold the hole, first sealing membrane is sealed the accommodation hole tip, the second sealing membrane is sealed advance the appearance runner, make hold the hole and with form the negative pressure in the advance appearance runner of accommodation hole intercommunication, when quick application of sample device, hold the board install in the sample board, the drainage needle is pierced liquid sample in the sample acceptors is followed advance appearance runner and flow into each respectively advance each hold the hole.

2. The rapid sample addition device of claim 1, wherein: the number of the sample injection flow passages is the same as that of the containing holes, each sample injection flow passage extends from the bottom of the outer wall of the containing hole to the position close to the top, and the sample injection flow passages are communicated with the inside of the containing hole at the position close to the top of the containing hole.

3. The rapid sample addition device of claim 2, wherein: the inner wall of the accommodating hole is provided with a bulge and a recess, the bulge and the recess are correspondingly arranged and are folded to form a sample injection flow passage, and the top of the sample injection flow passage is communicated with the accommodating hole.

4. A rapid prototyping apparatus according to claim 3 wherein: the accommodating hole is of a hollow structure and comprises a bottom surface, the sample injection runner extends to the bottom surface and forms a through hole on the bottom surface, and the second sealing film seals the through hole.

5. The rapid sample addition device of claim 1, wherein: the liquid guiding needle is of a hollow structure.

6. The rapid sample addition device of claim 5, wherein: the liquid-guiding needle is provided with a notch, the notch extends along the extending direction of the liquid-guiding needle, and the inside of the liquid-guiding needle is communicated with the sample accommodating part through the notch.

7. The rapid sample addition device of claim 5, wherein: the end part of the liquid guiding needle is conical.

8. The rapid sample addition device of claim 1, wherein: the sample plate is also provided with a sample adding groove, a liquid inlet, an overflow port and a waste liquid groove, wherein the sample adding groove is communicated with the sample accommodating part through the liquid inlet, and the sample accommodating part is communicated with the waste liquid groove through the overflow port.

9. The rapid prototyping device of claim 8 wherein: the bottom of the sample adding tank is obliquely arranged, and the lowest part of the bottom of the sample adding tank is connected with the bottom of the liquid inlet.

10. The rapid sample addition device of claim 1, wherein: the sample accommodating part is provided with a plurality of sample holes, and a plurality of sample holes are communicated through a main flow channel.