CN213632903U

CN213632903U - Reagent device

Info

Publication number: CN213632903U
Application number: CN202022532581.7U
Authority: CN
Inventors: 王志平
Original assignee: Shenzhen Anlu Medical Technology Co ltd
Current assignee: Shenzhen Anlu Medical Technology Co ltd
Priority date: 2020-11-05
Filing date: 2020-11-05
Publication date: 2021-07-06
Anticipated expiration: 2030-11-05

Abstract

In a reagent device; a sampling nozzle in the sampling device punctures a sealing film at the top of a first container after sampling, and the sample is mixed with a first reagent in the first container to finish primary dyeing; the sampling device and the first container are continuously pushed or rotated downwards, so that the puncturing device is contacted with and punctures the bottom sealing film, and the sample mixed solution after the first dyeing is mixed with a second reagent in the second container to form a sample mixed solution after the second dyeing; invert sampling device together with first container and second container, and the mixed liquid tiling device is dripped into from the play appearance mouth to the sample mixed liquid after the second dyeing via second container, first container, sampling mouth, sample holding passageway in proper order, and the mixed liquid is flat in mixed liquid tiling device and is used for the microscopic examination. The reagent device has the advantages of simple and ingenious structure, convenient and efficient secondary dyeing, simple operation, good cell dyeing effect, obvious cell characteristics after dyeing and suitability for cell analysis in a bright field.

Description

Reagent device

Technical Field

The application belongs to the field of cell staining technology and device, in particular relates to the field of cell staining technology and device before cell detection in body fluid or secretion of an organism, and in particular relates to a staining device, a staining reagent and a staining method for staining cells in body fluid or secretion.

Background

In the cell detection of body fluids or secretions of living organisms, cell staining techniques are generally used to distinguish between different types, forms and states of cells in body fluids or secretions.

In the conventional cell staining technique for brightfield observation and detection, a sample to be detected is usually smeared on a transparent carrier such as a glass slide, after the sample to be detected is dried, a dye solution and a buffer solution with different staining effects are sequentially dropped, after the dye solution and the buffer solution are uniformly mixed and dyed, a main body of the dye solution and the buffer solution influencing the observation is washed away, after the sample to be dyed is dried, the whole dyeing process is completed, and a stained sample smear for brightfield observation is formed.

FIG. 32 is a schematic diagram showing a process of preparing a blood smear according to the prior art, which includes steps of preparing the blood smear and steps of staining the blood smear.

The step of making the blood smear comprises four small steps: respectively, step 1.1: dropping the blood sample onto the glass slide; step 1.2: taking another glass slide, inclining the glass slide according to an angle of 30-45 degrees, and pushing the glass slide to the other side at a constant speed; step 1.3: the blood film is evenly coated, and is in a flame shape to be a qualified smear; step 1.4: wait for the blood film to dry and prepare for staining.

The step of smear staining includes five small steps: respectively, step 2.1: marking with a pencil, flatly placing the blood smear on a dyeing rack, and marking with a crayon to prevent liquid from overflowing; step 2.2: naturally drying the blood smear, dripping 1-2 drops of dyeing liquid to cover the blood smear, and dyeing for about 1 minute; step 2.3: dropwise adding a little more buffer solution with the volume of 2-4 drops), and uniformly blowing the buffer solution and the dye solution by using an aurilave for about 5 minutes; step 2.4: gently shake the slide and then flush the stain from one side of the slide with a fine stream of tap water (note, need not pour the stain first and then flush) for about 1 minute; step 2.5: and stopping washing, and naturally drying the blood slices or sucking the blood slices by using filter paper to dry the blood slices, so that the blood slices can be used for microscopic examination.

As can be seen from the above steps, the staining steps in the prior art are extremely tedious and time-consuming, and the operation of each step requires a trained professional operator to prepare a qualified observation sample. The staining efficiency in the prior art is extremely low, and the cell staining of a sample cannot be carried out on a large scale.

Disclosure of Invention

The technical problem to be solved by the application is to avoid the defects in the prior art, provide a simple and efficient reagent device and a secondary dyeing method, and be suitable for large-scale and large-batch living body dyeing of sample cells.

The technical scheme for solving the problems is that the reagent device is used for secondary treatment of a sample and comprises a first container and at least one second container; a first container for containing a first reagent for a first sample processing; a second container for holding a second reagent for a second sample processing; a sealing film is arranged at the top of the first container; the bottom of the first container is provided with a sealing film; the upper part of the inner wall of the second container is provided with a puncture device; the first container bottom and the second container top are detachably connected; in a first state, the lancing device does not contact the carrier film, and the first reagent in the first container and the second reagent in the second container are each independently stored; in a second state, the lancing device contacts and punctures the back cover membrane and the first reagent in the first container and the second reagent in the second container are mixed.

The reagent device also comprises a sampling device, wherein the sampling device comprises a sampling device main body, a sampling nozzle, a sample accommodating channel and a sample outlet nozzle; the sampling nozzle, the sample accommodating channel and the sample outlet nozzle are communicated, the sampling nozzle is arranged at one end of the sampling device main body, and the sample outlet nozzle is arranged at the other end of the sampling device main body; the sampling nozzle is used for loading an external sample into the sampling device; the sampling nozzle is also used for puncturing the sealing film, delivering a sample obtained by sampling into the first container, mixing the sample with the first reagent in the first container to complete the first treatment of the sample, and storing the sample mixed solution after the first treatment in the first container.

The first container and the second container are both hollow columns; the first container comprises a first container body and a first container lower part; the first container main body is communicated with the lower part of the first container; the outer diameter of the lower part of the first container is less than or equal to the inner diameter of the second container; the outer diameter of the first container main body is larger than or equal to the outer diameter of the lower part of the first container; in the first state, the lower part of the first container is inserted into the upper part of the second container, and the bottom sealing film at the bottom of the first container enters the second container, but the bottom sealing film is not contacted with the puncturing device; in the second state, the lower portion of the first container is inserted further down into the interior of the second container, the carrier film is contacted by the puncturing device, and the puncturing device contacts and punctures the carrier film.

A first thread structure is arranged on the outer wall of the bottom of the first container; a second thread structure is arranged on the inner wall of the top of the second container; the relative position between the sealing base film and the puncturing device, namely the contact state of the sealing base film and the puncturing device can be adjusted by adjusting the matching state of the first thread structure and the second thread structure; the first thread structure and the second thread structure are in a first matching state, the puncturing device does not contact the sealing membrane, and the first reagent in the first container and the second reagent in the second container are respectively and independently stored; and under the second matching state of the first thread structure and the second thread structure, the puncture device contacts and punctures the back sealing film, and the first reagent in the first container is mixed with the second reagent in the second container.

The puncturing device comprises a barb; one end of the barb is fixedly connected with the inner wall of the second container; the other end of the barb is in a peak shape protruding from bottom to top.

The second container is open at the top.

A second container sealing film is also arranged at the top of the second container; in a first state, the piercing device does not contact the closure membrane and the second container closure membrane, and the first reagent in the first container and the second reagent in the second container are each independently stored; in the second state, the puncturing device sequentially contacts and punctures the second container sealing film and the bottom sealing film, and the first reagent in the first container and the second reagent in the second container are mixed.

The puncturing device comprises two symmetrically arranged barbs; the distance between the peak-shaped tops of the two barbs is less than or equal to the diameter of the back cover film.

When the first container and the second container are connected, the sampling device is inserted into the first container, the puncturing device contacts and punctures the back cover film, and the sample mixed solution after the first treatment in the first container and the second reagent in the second container are mixed to form a sample mixed solution after the second treatment; inverting the sampling device together with the first container and the second container, and enabling the sample mixed liquid after the second treatment to sequentially flow out of the sample outlet through the second container, the first container, the sampling nozzle and the sample accommodating channel; the sampling nozzle is a quantitative sampling nozzle, and the sample outlet nozzle is a quantitative sample outlet nozzle; the quantitative amount of the sampling nozzle, the capacity of the first container and the capacity of the second container are matched with each other.

The sampling device is a quantitative sampling device, and the volume range of quantitative sampling is 5 microliters to 15 microliters.

The sampling device is a quantitative sampling device, and the volume range of quantitative sampling is 7.5 microliters to 10 microliters.

The sample outlet nozzle is a quantitative sample outlet nozzle, and the quantitative sample outlet capacity ranges from 25 microliters to 35 microliters.

The first container has a volume in the range of 600 microliters to 1895 microliters.

The second container has a capacity in the range of 250 microliters to 2245 microliters.

The reagent device also comprises a protective cap which is sleeved at one end of the sampling device and is used for covering the sample outlet nozzle; the protective cap prevents the mixed liquid in the first container from flowing out of the sample outlet nozzle.

Compared with the prior art, the beneficial effect of this application is: 1. the two staining reagents are respectively accommodated in different spaces, which is beneficial to better preservation of the reagents; the shelf life can be greatly prolonged; 2. if two kinds of dyeing liquid are needed to be used in the dyeing reaction, the dyeing process can be normally carried out only by puncturing the bottom sealing film of the first container when the secondary dyeing reaction is needed; 3. the reagent device in the application has a simple and ingenious structure, is very convenient to carry out efficient secondary dyeing, is simple to operate, has a good cell dyeing effect, has obvious cell characteristics after dyeing, and is suitable for cell analysis in a bright field; 4. the secondary dyeing method is simple to operate, low in requirement on operators, uniform in dyeing and short in time consumption, and can realize a foolproof dyeing process; the defects of complex cell dyeing operation, high professional requirement, long time consumption, uneven dyeing and high dyeing material cost in the prior art are overcome. The dyeing operation is simple, and foolproof dyeing operation can be realized, so that the dyeing device is suitable for various application scenes. The scene such as the instant check is also suitable for a plurality of application scenes such as emergency treatment, bedside, battlefield and the like which lack large professional equipment, professionals and complex check environments.

Drawings

FIG. 1 is a schematic side elevation view of one embodiment of a reagent apparatus in an assembled state;

FIG. 2 is one of the schematic axonometric views of one of the embodiments of the reagent apparatus in a disassembled state;

FIG. 3 is a second schematic isometric view of one embodiment of a reagent apparatus in a disassembled state;

FIG. 4 is a schematic top elevational view of one embodiment of a reagent apparatus in an assembled state;

FIG. 5 is a schematic cross-sectional view of AA of FIG. 4;

FIG. 6 is a schematic isometric projection of a first container;

FIG. 7 is a schematic top elevational view of the first container;

FIG. 8 is a schematic cross-sectional view of BB of FIG. 7;

FIG. 9 is a schematic isometric projection of a second container;

FIG. 10 is a schematic top elevational view of a second container;

FIG. 11 is a schematic cross-sectional view of the CC of FIG. 10;

FIG. 12 is a schematic axonometric view of the sampling device and the protective cap in a disassembled state;

FIG. 13 is one of the schematic axonometric projections of the sampling device and the protective cap in the assembled state;

fig. 14 is a schematic top orthographic view of the sampling device and the protective cap in a combined state;

FIG. 15 is a schematic sectional view of the DD of FIG. 14;

FIG. 16 is a schematic isometric projection of the sampling device, protective cap, first container, and second container in an exploded state;

FIG. 17 is one of the schematic axonometric projections of the sampling device, the protective cap, the first container and the second container in a combined state;

FIG. 18 is a schematic top orthographic view of the sampling device, the cap, the first container and the second container in an assembled state;

FIG. 19 is a schematic cross-sectional view of EE of FIG. 18;

FIG. 20 is a schematic sectional view of the EE in a disassembled state of the sampling device and the cap relative to the first and second containers;

FIG. 21 is one of the schematic cross-sectional EE views of a sampling device, a cap, a first container and a second container in an assembled state; in the figure, the sampling device has punctured the sealing membrane of the first container and has entered the interior of the first container;

FIG. 22 is a second schematic sectional view of the EE in an assembled state of the sampling device, the cap, the first container and the second container; in the figure, the sampling device has punctured the sealing membrane of the first container and has entered the interior of the first container; simultaneously, the puncturing device in the second container contacts and punctures the sealing membrane at the bottom of the first container;

FIG. 23 is a second schematic perspective view of the sampling device and the protective cap in an assembled state; in the figure, the sampling nozzle does not suck a sample and is in an unsampled state;

fig. 24 is a third schematic axonometric view of the sampling device and the protective cap in the assembled state; the sample is drawn into the nozzle in a sampled state, and the sample is taken at 60;

FIG. 25 is one of the schematic cross-sectional EE views of a sampling device and cap combination, a first container and a second container, but in a separated state; the arrow direction in the figure is a direction of pressing down the first container 10;

FIG. 26 is a second schematic sectional view of the EE in an assembled state of the sampling device, the cap, the first container and the second container;

in fig. 25 and 26, the sample is drawn into the sampling nozzle in a sampled state, and the sample is sampled at 60; reference numeral 61 is a first reagent; reference numeral 62 is a second reagent;

FIG. 27 is a third schematic sectional view of the EE in an assembled state of the sampling device, the cap, the first container and the second container; in fig. 27, the sample in the sampling nozzle has entered the first container and mixed with the first solvent and shaken up to form a sample mixture 63 after the first treatment; the direction of the arrow in the figure is the direction of downward rotation of the first container and also the direction of rotation during mixing and shaking;

FIG. 28 is a third schematic sectional view of the EE in an assembled state of the sampling device, the cap, the first container and the second container; the sampling device punctures the sealing film of the first container and enters the first container; simultaneously, the puncturing device in the second container contacts and punctures the sealing membrane at the bottom of the first container; the sample mixed solution 63 after the first treatment enters a second container and is subjected to secondary sample treatment with a second reagent 62;

FIG. 29 is a third schematic sectional view of the EE in an assembled state of the sampling device, the cap, the first container and the second container; with respect to fig. 28, the second container of fig. 29 has been thoroughly mixed to form a second treated sample mixture 64;

fig. 30 is a schematic view showing a process of dropping the sample mixture 64 after the secondary treatment into the mixture tiling device 70 in the assembled state of the sampling device, the protective cap, the first container, and the second container; in the figure, reference numeral 71 is a main body of the mixed liquid spreading device, and reference numeral 72 is a mixed liquid dropping area; reference numeral 75 is a cover sheet covering the mixed liquid spreading area;

FIG. 31 is a schematic representation of the steps of one embodiment of a cell staining method contemplated by the present application;

FIG. 32 is a schematic view showing a process of preparing a blood smear in the prior art.

Detailed Description

Embodiments of the present application will be described in further detail below with reference to the drawings.

FIG. 32 is a schematic diagram showing a process of preparing a blood smear according to the prior art, which includes steps of preparing the blood smear and steps of staining the blood smear. As can be seen from the steps in fig. 1, the dyeing operation in the prior art is complex in steps, complicated and time-consuming in operation, and the operation of each step requires a trained professional operator, so that a qualified observation sample can be obtained. The dyeing efficiency in the prior art is extremely low, and the sample preparation of morphological bright field detection can not be carried out on a large scale.

Further, in the staining procedure of the prior art, since the sample is subjected to the processes of smearing, spreading and drying, most of the cells in the sample to be stained are "killed" during the staining process, and thus the staining is only in a state that most of the cells are inactivated, so that the stained smear cannot reflect the physiological state of the cells in the body fluid more truly. Furthermore, the dyeing liquid in the prior art is complex in preparation process and high in cost.

As shown in fig. 1 to 5, a reagent set embodiment for secondary processing of a sample includes a first container 10 and a second container 20; a first container 10 for containing a first reagent for a first sample processing; a second container 20 for holding a second reagent for a second sample processing; a sealing film 11 is arranged at the top of the first container 10; the first container bottom is provided with a sealing membrane 12. The first container 10 and the second container 20 are respectively used for accommodating reagents with different functions, such as staining solution, and the form can ensure that the reagents with different functions, such as staining solution, have longer storage stability. Generally, if the first and second reagents are mixed, the shelf life of the mixed reagents is reduced.

As shown in fig. 6 to 11, the first container 10 and the second container 20 are both hollow cylindrical; the second container 20 is open at the top. The first container 10 includes a first container body 18 and a first container lower portion 13; the first container main body 18 and the first container lower part 13 are communicated; the outer diameter of the lower part 13 of the first container is less than or equal to the inner diameter of the second container 20; the outer diameter of the first container body 18 is equal to or larger than the outer diameter of the first container lower part 13; in the first state, the lower portion 13 of the first container is inserted into the upper portion of the second container 20, and the bottom sealing film 12 at the bottom of the first container 10 enters the second container 20, but the bottom sealing film 12 and the puncturing device 21 are not in contact with each other; in the second state, the first container lower portion 13 is further inserted downward into the interior of the second container 20, the back cover film 12 contacts the puncture device 21, and the puncture device 21 contacts and punctures the back cover film 12.

The lower portion 13 of the first container may be inserted downwardly into the interior of the second container 20 in a variety of ways, one of which is by directly pressing the first container 10 downwardly and the other of which is by threading or other structure, the first container 10 being rotated downwardly to bring the sealing membrane at the bottom of the first container 10 into contact with the piercing device 21.

In some embodiments not shown in the drawings, the outer wall of the first container bottom is provided with a first thread structure; a second thread structure is arranged on the inner wall of the top of the second container; the relative position between the sealing base film and the puncturing device, namely the contact state of the sealing base film and the puncturing device can be adjusted by adjusting the matching state of the first thread structure and the second thread structure; the first thread structure and the second thread structure are in a first matching state, the puncturing device does not contact the sealing membrane, and the first reagent in the first container and the second reagent in the second container are respectively and independently stored; and under the second matching state of the first thread structure and the second thread structure, the puncture device contacts and punctures the back sealing film, and the first reagent in the first container is mixed with the second reagent in the second container.

In some embodiments not shown in the drawings, the puncturing device comprises a barb; one end of the barb is fixedly connected with the inner wall of the second container; the other end of the barb is in a peak shape protruding from bottom to top.

The barb and the thread design make the wall between first container and second container break more thoroughly, are favorable to the intensive mixing of reagent.

In some embodiments not shown in the drawings, the second container top is also provided with a second container closure film; in a first state, the piercing device does not contact the closure membrane and the second container closure membrane, and the first reagent in the first container and the second reagent in the second container are each independently stored; in the second state, the puncturing device sequentially contacts and punctures the second container sealing film and the bottom sealing film, and the first reagent in the first container and the second reagent in the second container are mixed. The second container sealing film can enable the second container to finish the sealing and packaging of the internal reagent without depending on the first container, so that the dependence between the first container and the second container is reduced, the independence of the first container and the second container is enhanced, and the free combination of two containers containing different reagents is facilitated. I.e. the second container may contain a different reagent to cooperate with the reagent in the first container.

As shown in fig. 9 to 11, in an embodiment of the reagent set for secondary processing of a sample, the second container 20 is open at the top, and the piercing means 21 is provided on the upper part of the inner wall of the second container; the first container bottom and the second container top are detachably connected; in the first state, the piercing means 21 does not contact the sealing membrane 12, and the first reagent in the first container 10 and the second reagent in the second container 20 are each independently stored; in the second state, the puncturing device 21 contacts and punctures the back cover film 12, and the first reagent in the first container 10 and the second reagent in the second container 20 are mixed. The puncturing device 21 comprises two symmetrically arranged barbs 211; one end of the barb 211 is fixedly connected with the inner wall of the second container 20, and reference numeral 212 in fig. 11 is the connection part of the barb and the inner wall of the second container; the other end of the barb 211 has a peak shape protruding from the bottom to the top. The distance between the peak-shaped tops of the two barbs is less than or equal to the diameter of the back cover film. The puncturing device 21 comprises two symmetrically arranged barbs 211; the distance between the peak-shaped tops of the two barbs is less than or equal to the diameter of the back cover film. When the relative positions of the first container 10 and the second container 20 are changed, such as pressing the first container 10 downward while rotating the first container 10 relative to the second container 20, the two symmetrically arranged barbs 211 or one barb will cut the back cover film 12 in a rotating manner, so that the whole or most of the back cover film 12 is cut, and the reagent in the first container 10 can enter the second container 20 more smoothly. The design can better puncture the sealing base film, so that the reagents are fully and quickly mixed.

As in the embodiment of fig. 12-15, further comprising a sampling device 30, the sampling device 30 comprises a sampling device body 31, a sampling nozzle 32, a sample accommodating channel 35, and a sample outlet nozzle 33; the sampling nozzle 32, the sample accommodating channel 35 and the sample outlet nozzle 33 are communicated, the sampling nozzle 32 is arranged at one end of the sampling device main body 31, and the sample outlet nozzle 33 is arranged at the other end of the sampling device main body 31; the sampling nozzle 32 is used to load an external sample into the sampling device 30; the sampling nozzle 32 is further configured to pierce the sealing film 12, transfer the sample obtained by sampling into the first container 10, mix the sample with the first reagent in the first container 10 to complete the first treatment of the sample, and store the sample mixture after the first treatment in the first container 10. The quantitative blood sampling device is used for quantitative blood sampling and quantitative dripping of reaction liquid, the quantitative blood sampling and quantitative sample dripping processes are simplified, one component can complete multiple functions, and the efficiency of the dyeing process is improved.

In the embodiment of fig. 12-15, the sampling device is a quantitative sampling device with a volume of quantitative sampling in the range of 5 microliters to 15 microliters. The sampling device is a quantitative sampling device, and the volume range of quantitative sampling is 7.5 microliters to 10 microliters. The sample outlet nozzle is a quantitative sample outlet nozzle, and the quantitative sample outlet capacity ranges from 25 microliters to 35 microliters. The first container has a volume in the range of 600 microliters to 1895 microliters. The second container has a capacity in the range of 250 microliters to 2245 microliters.

The quantitative sampling design is convenient for subsequent analysis and treatment after cell staining, the volume of 1 drop of blood is 7.5-10 ul, the blood sampling volume is small, the precision is poor, the sampling volume exceeds 1 drop of blood, and the design requirement of the device is high; the first container and the second container can be matched with a quantitative sampling device in capacity and are suitable for manual operation, and the capacity range is the volume design of the most suitable container size suitable for being mastered by hands.

In the embodiments of fig. 12 to 15 and 16 to 24, the sampling device 30 further comprises a protective cap 50, the protective cap 50 is sleeved on one end of the sampling device 30 for covering the sampling nozzle 33; the protective cap 50 prevents the mixed liquid in the first container from flowing out of the outlet nozzle 33. When the sampling device 30 is inserted into the first container 10 and the sampling device 30 together with the first container 10 or the sampling device 30 together with the first container 10 and the second container 20 is inverted or mixed and shaken, that is, when the sampling nozzle 33 is directed downward, the mixed liquid in the first container 10 easily flows out from the sampling nozzle 33; the cap prevents the mixed liquid in the first container 10 from flowing out of the outlet nozzle 33 during the above operation. The protective cap is a leak-proof component of the sampling device, and inconvenience caused by leakage of reaction liquid is avoided in the process of uniformly mixing the sample with the reagent.

As shown in fig. 16 to 20, when the first container 10 and the second container 20 are connected, the sampling device 30 is inserted into the first container 20, and the puncturing device 21 contacts and punctures the back cover film 12, the first processed sample mixed solution 61 in the first container and the second reagent in the second container are mixed to form a second processed sample mixed solution 62; inverting the sampling device together with the first container and the second container, and enabling the sample mixed solution 64 after the second treatment to sequentially flow out of the sample outlet nozzle through the second container 20, the first container 10, the sampling nozzle and the sample accommodating channel; the sampling nozzle is a quantitative sampling nozzle, and the sample outlet nozzle is a quantitative sample outlet nozzle; the quantitative amount of the sampling nozzle, the capacity of the first container and the capacity of the second container are matched with each other.

In some embodiments not shown in the drawings, the second container 20 has two or more. The plurality of second containers 20 are connected in sequence; a plurality of second containers 20 for storing reagents required for the multi-step sample processing, respectively; the second container 20 at the bottom can store the reagents required for the last treatment; the bottom most second container 20 may be empty and used to hold the final sample after processing. The volumes of the second containers 20 may be different, and are used for storing reagents with different dosages, and simultaneously reserving a space for the sampling device to enter, and introducing the volume of the mixed liquid in the previous link through the sampling device; i.e., the volume of each second container 20, is required to simultaneously contain the reagents, sampling device and the mixed solution of the previous step.

An embodiment of a secondary staining method, not shown in some of the figures, for secondary staining of cells in a biological sample, based on the reagent device described above; the first reagent in the first container is a first staining reagent for a first sample staining process; the first reagent in the second container is a second staining reagent for a second staining process of the sample; the secondary dyeing method comprises the following steps: the method comprises the following steps: carrying out quantitative sampling on a sample to be dyed by using a sampling device; step two: injecting a quantitative sample to be dyed into a first container by using the sampling device in the first step for first dyeing, and finishing the first dyeing after uniformly mixing; step three: and changing the relative position relationship between the first container and the second container to enable the puncturing device to contact and puncture the sealing membrane, mixing the mixed solution after the first dyeing in the first container and the second dyeing reagent in the second container for the second dyeing, and finishing the second dyeing after the mixed solution is uniformly mixed. The method also comprises the following four steps: and (4) quantitatively dripping the mixed solution of the sample obtained in the third step after the second dyeing from a sample outlet.

In one embodiment of a secondary staining method as shown in FIG. 31, a sampling or dripping device is used to quantitatively take blood; inserting a sampling or dripping device into a reagent loading device, and reversing and uniformly mixing for one minute; rotating the upper half part of the reagent device, namely the first container, to enable the first container to face downwards and break through the sealing membrane of the first container, enabling the sample mixed solution in the first container to enter the second container, and reversing and uniformly mixing for 1 minute; and removing the protective cap at the tail part of the sampling or sample dripping device, inverting the sampling or sample dripping device together with the first container and the second container, and pouring out the sample mixed liquid after secondary mixing from a sample outlet nozzle of the sampling or sample dripping device.

In the secondary dyeing method of the present application, a first container and a second container are used for accommodating a first reagent and a second reagent, respectively; a first reagent for a first staining; the second reagent is used for the second staining. The two staining reagents are respectively contained in different spaces, which is beneficial to better preservation of the reagents. The shelf life of the separately stored reagents is greatly extended compared to the mixed solution of the first reagent and the second reagent. If two dyeing liquids are needed to be used in the dyeing reaction, the dyeing process can be normally carried out only by puncturing the bottom sealing film of the first container when the secondary dyeing reaction is needed.

In the cell staining method, the cells in the sample to be stained are stained in a liquid suspension, and the environment for the staining solution and the dilution is relatively close to the biological physiological state, so that the cell activity is better maintained in the staining process.

In the reagent device and the secondary dyeing method thereof; a sampling nozzle in the sampling device punctures a sealing film at the top of a first container after sampling, and the sample is mixed with a first reagent in the first container to finish primary dyeing; the sampling device and the first container are continuously pushed or rotated downwards, so that the puncturing device is contacted with and punctures the bottom sealing film, and the sample mixed solution after the first dyeing is mixed with a second reagent in the second container to form a sample mixed solution after the second dyeing; invert sampling device together with first container and second container, and the mixed liquid tiling device is dripped into from the play appearance mouth to the sample mixed liquid after the second dyeing via second container, first container, sampling mouth, sample holding passageway in proper order, and the mixed liquid is flat in mixed liquid tiling device and is used for the microscopic examination. The reagent device has the advantages of simple and ingenious structure, convenient and efficient secondary dyeing, simple operation, good cell dyeing effect, obvious cell characteristics after dyeing and suitability for cell analysis in a bright field.

The reagent device and the secondary dyeing method thereof have the advantages of simple operation, low requirement on operators, uniform dyeing, short time consumption and capability of realizing a foolproof dyeing process; the defects of complex cell dyeing operation, high professional requirement, long time consumption, uneven dyeing and high dyeing material cost in the prior art are overcome. The dyeing operation is simple, and foolproof dyeing operation can be realized, so that the dyeing device is suitable for various application scenes. The scene such as the instant check is also suitable for a plurality of application scenes such as emergency treatment, bedside, battlefield and the like which lack large professional equipment, professionals and complex check environments.

The above description is only an example of the present application and is not intended to limit the scope of the present application, and all modifications of equivalent structures and equivalent processes, which are made by the contents of the present specification and the accompanying drawings, or which are directly or indirectly applied to other related technical fields, are intended to be included within the scope of the present application.

Claims

1. A reagent device for secondary processing of a sample,

comprising a first container and at least one second container; a first container for containing a first reagent for a first sample processing; a second container for holding a second reagent for a second sample processing; a sealing film is arranged at the top of the first container; the bottom of the first container is provided with a sealing film;

the upper part of the inner wall of the second container is provided with a puncture device; the first container bottom and the second container top are detachably connected;

in a first state, the lancing device does not contact the carrier film, and the first reagent in the first container and the second reagent in the second container are each independently stored;

in a second state, the lancing device contacts and punctures the back cover membrane and the first reagent in the first container and the second reagent in the second container are mixed.

2. Reagent device according to claim 1,

the sampling device comprises a sampling device body, a sampling nozzle, a sample accommodating channel and a sample outlet nozzle;

the sampling nozzle, the sample accommodating channel and the sample outlet nozzle are communicated, the sampling nozzle is arranged at one end of the sampling device main body, and the sample outlet nozzle is arranged at the other end of the sampling device main body;

the sampling nozzle is used for loading an external sample into the sampling device;

the sampling nozzle is also used for puncturing the sealing film, delivering a sample obtained by sampling into the first container, mixing the sample with the first reagent in the first container to complete the first treatment of the sample, and storing the sample mixed solution after the first treatment in the first container.

3. Reagent device according to claim 1 or 2,

the first container and the second container are both hollow columns;

the first container comprises a first container body and a first container lower part; the first container main body is communicated with the lower part of the first container; the outer diameter of the lower part of the first container is less than or equal to the inner diameter of the second container; the outer diameter of the first container main body is larger than or equal to the outer diameter of the lower part of the first container;

in the first state, the lower part of the first container is inserted into the upper part of the second container, and the bottom sealing film at the bottom of the first container enters the second container, but the bottom sealing film is not contacted with the puncturing device;

in the second state, the lower portion of the first container is inserted further down into the interior of the second container, the carrier film is contacted by the puncturing device, and the puncturing device contacts and punctures the carrier film.

4. Reagent device according to claim 1 or 2,

a first thread structure is arranged on the outer wall of the bottom of the first container; a second thread structure is arranged on the inner wall of the top of the second container;

the relative position between the sealing base film and the puncturing device, namely the contact state of the sealing base film and the puncturing device can be adjusted by adjusting the matching state of the first thread structure and the second thread structure;

the first thread structure and the second thread structure are in a first matching state, the puncturing device does not contact the sealing membrane, and the first reagent in the first container and the second reagent in the second container are respectively and independently stored;

and under the second matching state of the first thread structure and the second thread structure, the puncture device contacts and punctures the back sealing film, and the first reagent in the first container is mixed with the second reagent in the second container.

5. Reagent device according to claim 1 or 2,

the puncturing device comprises a barb;

one end of the barb is fixedly connected with the inner wall of the second container; the other end of the barb is in a peak shape protruding from bottom to top.

6. Reagent device according to claim 1 or 2,

the second container is open at the top.

7. Reagent device according to claim 5,

a second container sealing film is also arranged at the top of the second container;

in a first state, the piercing device does not contact the closure membrane and the second container closure membrane, and the first reagent in the first container and the second reagent in the second container are each independently stored;

in the second state, the puncturing device sequentially contacts and punctures the second container sealing film and the bottom sealing film, and the first reagent in the first container and the second reagent in the second container are mixed.

8. Reagent device according to claim 5,

the puncturing device comprises two symmetrically arranged barbs;

the distance between the peak-shaped tops of the two barbs is less than or equal to the diameter of the back cover film.

9. Reagent device according to claim 2,

when the first container and the second container are connected, the sampling device is inserted into the first container, and the puncture device is made to contact and puncture the back cover film, and the sample mixed solution after the first treatment in the first container and the second reagent in the second container are mixed to form a sample mixed solution after the second treatment;

inverting the sampling device together with the first container and the second container, and enabling the sample mixed liquid after the second treatment to sequentially flow out of the sample outlet through the second container, the first container, the sampling nozzle and the sample accommodating channel;

the sampling nozzle is a quantitative sampling nozzle, and the sample outlet nozzle is a quantitative sample outlet nozzle; the quantitative amount of the sampling nozzle, the capacity of the first container and the capacity of the second container are matched with each other.

10. Reagent device according to claim 9,

11. Reagent device according to claim 10,

12. Reagent device according to claim 9,

13. Reagent device according to claim 9,

14. Reagent device according to claim 9,

15. Reagent device according to claim 2,

the sampling device is characterized by also comprising a protective cap which is sleeved at one end of the sampling device and is used for covering the sampling nozzle; the protective cap prevents the mixed liquid in the first container from flowing out of the sample outlet nozzle.