CN114451295A - Breeding method of maize hybrid with high white spot disease resistance - Google Patents
Breeding method of maize hybrid with high white spot disease resistance Download PDFInfo
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- CN114451295A CN114451295A CN202210180757.8A CN202210180757A CN114451295A CN 114451295 A CN114451295 A CN 114451295A CN 202210180757 A CN202210180757 A CN 202210180757A CN 114451295 A CN114451295 A CN 114451295A
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- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 42
- 238000009395 breeding Methods 0.000 title claims abstract description 35
- 206010027982 Morphoea Diseases 0.000 title claims abstract description 15
- 240000008042 Zea mays Species 0.000 title claims description 40
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 title claims description 5
- 235000009973 maize Nutrition 0.000 title claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 37
- 235000005822 corn Nutrition 0.000 claims abstract description 37
- 230000001488 breeding effect Effects 0.000 claims abstract description 29
- 238000000034 method Methods 0.000 claims abstract description 18
- 238000009396 hybridization Methods 0.000 claims description 14
- 238000004519 manufacturing process Methods 0.000 claims description 13
- 241000233647 Phytophthora nicotianae var. parasitica Species 0.000 claims description 12
- 201000010099 disease Diseases 0.000 claims description 10
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 10
- 241000196324 Embryophyta Species 0.000 claims description 9
- 241000607479 Yersinia pestis Species 0.000 claims description 9
- 235000013312 flour Nutrition 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 230000000306 recurrent effect Effects 0.000 claims description 6
- 239000002689 soil Substances 0.000 claims description 6
- ATROHALUCMTWTB-OWBHPGMISA-N phoxim Chemical compound CCOP(=S)(OCC)O\N=C(\C#N)C1=CC=CC=C1 ATROHALUCMTWTB-OWBHPGMISA-N 0.000 claims description 4
- 229950001664 phoxim Drugs 0.000 claims description 4
- ZOCSXAVNDGMNBV-UHFFFAOYSA-N 5-amino-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-4-[(trifluoromethyl)sulfinyl]-1H-pyrazole-3-carbonitrile Chemical compound NC1=C(S(=O)C(F)(F)F)C(C#N)=NN1C1=C(Cl)C=C(C(F)(F)F)C=C1Cl ZOCSXAVNDGMNBV-UHFFFAOYSA-N 0.000 claims description 3
- 241001124076 Aphididae Species 0.000 claims description 3
- 241000238631 Hexapoda Species 0.000 claims description 3
- 241000346285 Ostrinia furnacalis Species 0.000 claims description 3
- 230000009418 agronomic effect Effects 0.000 claims description 3
- 230000010154 cross-pollination Effects 0.000 claims description 3
- 210000005069 ears Anatomy 0.000 claims description 3
- 239000000839 emulsion Substances 0.000 claims description 3
- 238000013095 identification testing Methods 0.000 claims description 3
- 238000002955 isolation Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 239000000575 pesticide Substances 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 238000000746 purification Methods 0.000 claims description 3
- 238000012360 testing method Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 239000005899 Fipronil Substances 0.000 claims description 2
- 229940013764 fipronil Drugs 0.000 claims description 2
- 239000002917 insecticide Substances 0.000 claims description 2
- 241000209149 Zea Species 0.000 abstract 2
- 208000035240 Disease Resistance Diseases 0.000 description 4
- 241001635598 Enicostema Species 0.000 description 2
- 201000004569 Blindness Diseases 0.000 description 1
- 208000003643 Callosities Diseases 0.000 description 1
- 108700003861 Dominant Genes Proteins 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 108700005079 Recessive Genes Proteins 0.000 description 1
- 102000052708 Recessive Genes Human genes 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 210000004914 menses Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
- A01H1/122—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- A01H1/1245—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, e.g. pathogen, pest or disease resistance
Abstract
The invention discloses a method for breeding corn hybrid with high white spot disease resistance, which belongs to the technical field of corn hybrid breeding methods.
Description
Technical Field
The invention relates to the technical field of a corn hybrid breeding method, in particular to a corn hybrid breeding method with high white spot disease resistance.
Background
The corn belongs to the crop of both grain, menses and feed, has wide transformation and utilization range, large popularization and planting area, good development and application prospect, however, the main problems influencing the development of the corn in production at present are low yield, large and unstable performance difference among years and lack of high-yield corn varieties with good stress resistance, and the problem to be solved is that good corn varieties with high yield, high quality and good stress resistance are cultivated by utilizing hybrid advantages only from the perspective of heredity, new corn hybrid varieties are large and unstable in performance difference among years, incomplete in stress resistance and incapable of being effectively overcome by certain diseases, although the hybrid bred by hybridization has yield superiority, the hybrid bred by hybridization cannot resist leaf surface diseases and root diseases simultaneously, has poor quality and adaptability, the method is related to the lack of identification conditions of various diseases in most breeding units in China, so that the blindness of preparing F1 combination is high; and also relates to the insufficient understanding of the expression rules of the dominant gene controlled disease resistance and the recessive gene controlled disease resistance in the first generation of hybrid by the breeding workers, so that the method for breeding the corn hybrid with high white spot disease resistance is provided.
Disclosure of Invention
The invention aims to provide a method for breeding a corn hybrid with high white spot disease resistance, which aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a method for breeding corn hybrid with high white spot disease resistance, which comprises the following steps:
s1: taking 82-1 as a female parent and taking a conventional variety used in production of wide adaptability, black shank resistance and white spot resistance as a recurrent parent, breeding a male sterile line of the recurrent parent with excellent quality, good adaptability and black shank resistance and white spot resistance through multi-generation backcross, selecting fertile land during breeding, and performing pest control in the seedling stage and the middle and later stages;
s2: then taking the fertile materials resisting the black shank and the white spot as original resources, breeding a genetically stable inbred pure line resisting the black shank and the white spot through multi-generation inbred purification according to the breeding target of 'good yellow falling and maturing characteristics in the field', selecting fertile land during breeding, and performing pest control in the seedling stage and the middle and later stages;
s3: taking the male sterile line cultivated in the step S1 as a female parent and the selfing pure line as a male parent as a basic material for hybridization breeding, simultaneously, using the male sterile line as the female parent and the selfing pure line as the male parent to prepare a hybridization combination, selecting fertile land during cultivation, performing pest control in the seedling stage and the middle and later stages, and performing resistance and economic character identification on different diseases respectively in the same year by using retransmission plots or areas and normal plots of different diseases in a production area;
s4: carrying out field test on the hybridization combination in the step S3, wherein the planting density is 3000-;
s5: hybridizing the female parent and the male parent ZH28 obtained in the step S4, thus obtaining ten groups of hybrid seeds, and carrying out a field identification test on the corn line of the primary hybrid variety;
s6: in the cultivation process, the superior plants are selected for manual bagging isolation before the silking and the flour scattering, the silking and the flour scattering are carried out in a manual cross pollination mode, and then the female ears are sleeved on the bags, so that the whole-plant high-white-spot-disease-resistant corn intercross is obtained, the corn line after the selfing, the hybridization and the intercrossing can obviously improve the yield and the quality of the seed production, the production cost of the seeds is reduced, the yield of the hybrid corn is high, the quality is good, the agronomic characters are neat, and the hereditary characters are stable.
Preferably, in the S1, S2 and S3 steps, 150 ml of 2000-time liquid of phoxim missible oil is used for irrigating and applying the phoxim missible oil to control soil insects per mu, and biological pesticides such as BT emulsion or 5% fipronil insecticide are used for irrigating and controlling corn borers and aphids in the middle and later periods, and meanwhile, the situation that corns cannot be planted around a planting area is guaranteed.
Preferably, the maximum water holding capacity of the soil in the field is kept between 50 and 60 percent when the corn line is planted in the field, and the planting environment temperature is kept between 20 and 30 ℃.
Preferably, in the step S5, the parent-parent row ratio is 1: 4, every 4 rows of female parents are alternately planted with 1 row of male parents, the female parents are reserved with 5000 plants/mu, and the male parents are reserved with 1250 plants/mu.
Compared with the prior art, the invention has the beneficial effects that:
the corn hybrid seed with high yield, high quality and good stress resistance bred by the method has strong lodging resistance, good field disease resistance and high resistance to big spot, gray spot, white spot and head smut.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a technical scheme that: a method for breeding corn hybrid with high white spot disease resistance, which comprises the following steps:
s1: taking 82-1 as a female parent and taking a conventional variety used in production of wide adaptability, black shank resistance and white spot resistance as a recurrent parent, breeding a male sterile line of the recurrent parent with excellent quality, good adaptability and black shank resistance and white spot resistance through multi-generation backcross, selecting fertile land during breeding, and performing pest control in the seedling stage and the middle and later stages;
s2: then taking the fertile materials resisting the black shank and the white spot as original resources, breeding a genetically stable inbred pure line resisting the black shank and the white spot through multi-generation inbred purification according to the breeding target of 'good yellow falling and maturing characteristics in the field', selecting fertile land during breeding, and performing pest control in the seedling stage and the middle and later stages;
s3: taking the male sterile line cultivated in the step S1 as a female parent and the selfing pure line as a male parent as a basic material for hybridization breeding, simultaneously, using the male sterile line as the female parent and the selfing pure line as the male parent to prepare a hybridization combination, selecting fertile land during cultivation, performing pest control in the seedling stage and the middle and later stages, and performing resistance and economic character identification on different diseases respectively in the same year by using retransmission plots or areas and normal plots of different diseases in a production area;
s4: carrying out field test on the hybridization combination in the step S3, wherein the planting density is 3000-;
s5: hybridizing the female parent and the male parent ZH28 obtained in the step S4, thus obtaining ten groups of hybrid seeds, and carrying out a field identification test on the corn line of the primary hybrid variety;
s6: in the cultivation process, the superior plants are selected for manual bagging isolation before the silking and the flour scattering, the silking and the flour scattering are carried out in a manual cross pollination mode, and then the female ears are sleeved on the bags, so that the whole-plant high-white-spot-disease-resistant corn intercross is obtained, the corn line after the selfing, the hybridization and the intercrossing can obviously improve the yield and the quality of the seed production, the production cost of the seeds is reduced, the yield of the hybrid corn is high, the quality is good, the agronomic characters are neat, and the hereditary characters are stable.
In the steps of S1, S2 and S3, the corn seed is cultivated, 150 ml of 2000-time liquid of phoxim missible oil is applied to each mu to prevent and control soil insects, biological pesticides such as BT emulsion or 5% fipronil core irrigation is adopted to prevent and control corn borers and aphids in the middle and later periods, and meanwhile, the situation that the corn cannot be planted around a planting area is ensured;
when the corn seed line is planted in the field, the maximum water holding capacity of the soil field is kept between 50 and 60 percent, and the planting environment temperature is kept between 20 and 30 ℃;
in the step S5, the row ratio of the parent row to the parent row is 1: 4, every 4 rows of female parents are alternately planted with 1 row of male parents, the female parents are reserved with 5000 plants/mu, and the male parents are reserved with 1250 plants/mu.
The corn hybrid seed with high yield, high quality and good stress resistance bred by the method has strong lodging resistance, good field disease resistance and high resistance to big spot, gray spot, white spot and head smut.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (4)
1. A method for breeding corn hybrid with high white spot disease resistance, which is characterized in that: the corn hybrid breeding method comprises the following specific steps:
s1: taking 82-1 as a female parent and taking a conventional variety used in production of wide adaptability, black shank resistance and white spot resistance as a recurrent parent, breeding a male sterile line of the recurrent parent with excellent quality, good adaptability and black shank resistance and white spot resistance through multi-generation backcross, selecting fertile land during breeding, and performing pest control in the seedling stage and the middle and later stages;
s2: then taking the fertile materials resisting the black shank and the white spot as original resources, breeding a genetically stable inbred pure line resisting the black shank and the white spot through multi-generation inbred purification according to the breeding target of 'good yellow falling and maturing characteristics in the field', selecting fertile land during breeding, and performing pest control in the seedling stage and the middle and later stages;
s3: taking the male sterile line cultivated in the step S1 as a female parent and the selfing pure line as a male parent as a basic material for hybridization breeding, simultaneously, using the male sterile line as the female parent and the selfing pure line as the male parent to prepare a hybridization combination, selecting fertile land during cultivation, performing pest control in the seedling stage and the middle and later stages, and performing resistance and economic character identification on different diseases respectively in the same year by using retransmission plots or areas and normal plots of different diseases in a production area;
s4: carrying out field test on the hybridization combination in the step S3, wherein the planting density is 3000-;
s5: hybridizing the female parent and the male parent ZH28 obtained in the step S4, thus obtaining ten groups of hybrid seeds, and carrying out a field identification test on the corn line of the primary hybrid variety;
s6: in the cultivation process, the superior plants are selected for manual bagging isolation before the silking and the flour scattering, the silking and the flour scattering are carried out in a manual cross pollination mode, and then the female ears are sleeved on the bags, so that the whole-plant high-white-spot-disease-resistant corn intercross is obtained, the corn line after the selfing, the hybridization and the intercrossing can obviously improve the yield and the quality of the seed production, the production cost of the seeds is reduced, the yield of the hybrid corn is high, the quality is good, the agronomic characters are neat, and the hereditary characters are stable.
2. The method for breeding the maize hybrid with high white spot disease resistance according to claim 1, wherein the method comprises the following steps: in the steps of S1, S2 and S3, the corn seed is cultivated, 2000-fold liquid of phoxim missible oil with the concentration of 150 milliliters per mu is used for preventing and controlling soil insects, biological pesticides such as BT emulsion or 5 percent fipronil insecticide is used for drenching and controlling corn borers and aphids in the middle and later periods, and meanwhile, the situation that the corn cannot be planted around the planting area is guaranteed.
3. The method for breeding the maize hybrid with high white spot disease resistance according to claim 1, wherein the method comprises the following steps: when the corn seed line is planted in the field, the maximum water holding capacity of the soil field is kept between 50 and 60 percent, and the planting environment temperature is kept between 20 and 30 ℃.
4. The method for breeding the maize hybrid with high white spot disease resistance according to claim 1, wherein the method comprises the following steps: in the step S5, the row ratio of the parent row to the parent row is 1: 4, every 4 rows of female parents are alternately planted with 1 row of male parents, the female parents are reserved with 5000 plants/mu, and the male parents are reserved with 1250 plants/mu.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210180757.8A CN114451295A (en) | 2022-02-25 | 2022-02-25 | Breeding method of maize hybrid with high white spot disease resistance |
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| CN202210180757.8A CN114451295A (en) | 2022-02-25 | 2022-02-25 | Breeding method of maize hybrid with high white spot disease resistance |
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| CN202210180757.8A Pending CN114451295A (en) | 2022-02-25 | 2022-02-25 | Breeding method of maize hybrid with high white spot disease resistance |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113475390A (en) * | 2021-08-24 | 2021-10-08 | 云南珍禾丰种业有限公司 | Breeding method of high-yield and high-quality silage corns |
| CN113475391A (en) * | 2021-08-24 | 2021-10-08 | 云南珍禾丰种业有限公司 | High-yield silage corn crossbreeding method |
| CN113508749A (en) * | 2021-08-24 | 2021-10-19 | 云南珍禾丰种业有限公司 | High-oil waxy corn hybridization breeding method |
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2022
- 2022-02-25 CN CN202210180757.8A patent/CN114451295A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113475390A (en) * | 2021-08-24 | 2021-10-08 | 云南珍禾丰种业有限公司 | Breeding method of high-yield and high-quality silage corns |
| CN113475391A (en) * | 2021-08-24 | 2021-10-08 | 云南珍禾丰种业有限公司 | High-yield silage corn crossbreeding method |
| CN113508749A (en) * | 2021-08-24 | 2021-10-19 | 云南珍禾丰种业有限公司 | High-oil waxy corn hybridization breeding method |
Non-Patent Citations (2)
| Title |
|---|
| 曹文英: "5个新品种引来400科技户", 《中国种业》 * |
| 闫海霞等: "夏玉米主要穗部性状与单株产量之间的相关和通径分析", 《山东农业科学》 * |
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Application publication date: 20220510 |