CN114441250A

CN114441250A - Sperm sample film-making device

Info

Publication number: CN114441250A
Application number: CN202011195611.8A
Authority: CN
Inventors: 黄栢城; 梅志刚; 袁云东; 华德运; 石义康
Original assignee: Shenzhen Reetoo Biotechnology Co Ltd
Current assignee: Shenzhen Reetoo Biotechnology Co Ltd
Priority date: 2020-10-30
Filing date: 2020-10-30
Publication date: 2022-05-06

Abstract

A semen sample slide-making device comprises a sample adding mechanism, a slide-making mechanism and a transfer mechanism for transferring and transporting slide glass; wherein, there are application of sample position and film-making position along the movement track definition of transport mechanism, application of sample mechanism moves to application of sample position in order to add pending sample on the slide glass in a controlled manner, and film-making mechanism then is located film-making position to the pending sample to on the slide glass carries out the film-making, thereby makes into a piece sample. Through the cooperation between each mechanism, can accomplish sample collection, application of sample and film-making action automatically in succession, realize automatic, the batchization film-making to the seminal fluid sample, be favorable to improving film-making efficiency and guaranteeing the quality of piece sample to the produced series of problems of manual film-making have been solved.

Description

Sperm sample film-making device

Technical Field

The invention relates to the technical field of medical detection, in particular to a semen sample slide making device.

Background

The sperm quality analysis is an important means for evaluating the male fertility and is also an experimental basis for diagnosing andrology diseases and observing curative effects, so the sperm quality analysis has very important guiding significance for scientific research and clinic. The sperm quality analysis generally comprises the items of detection and analysis such as the sperm physical property, the sperm motility, the sperm survival rate, the sperm morphology, the sperm DNA fragmentation degree and the like; among them, in the process of performing the items of detection and analysis, such as sperm survival rate, sperm morphology, sperm DNA fragmentation degree, etc., sperm samples need to be subjected to slide and staining in order to be better observed and analyzed.

At present, the slide preparation and dyeing of semen samples are mostly manually operated, so that the time and labor are wasted, the efficiency is low, the standard quantification cannot be realized, and the requirement of large-batch slide preparation cannot be met.

Disclosure of Invention

The invention mainly solves the problem of providing a semen sample slide device to realize the automatic slide of semen samples.

In one embodiment, a semen sample production device is provided, comprising:

the transfer mechanism is controlled to move, a sample adding position and a slide making position are defined along the movement track of the transfer mechanism, and the transfer mechanism is used for transferring and transporting the slide glass;

the sample adding mechanism controllably moves to a sample adding position and is used for adding a sample to be processed to the glass slide when the transport mechanism transfers the glass slide to the sample adding position, wherein the sample to be processed is a semen sample or is formed by mixing the semen sample and a reaction reagent; and

and the sheet making mechanism is positioned at the sheet making position and used for making the to-be-processed sample on the slide sheet into the sheet making position when the transfer mechanism transfers the slide sheet to the sheet making position.

In one embodiment, the transfer mechanism comprises a conveying mechanism and a transfer mechanism, the sheet making mechanism comprises a smear mechanism and a turnover mechanism, and the sheet making position comprises a coating position, a turnover position, a dip-dyeing position and an air drying position;

the conveying mechanism controllably moves, the sample adding position, the coating position and the overturning position are sequentially distributed along the movement track of the conveying mechanism, and the conveying mechanism is used for bearing and conveying glass slides;

the smear mechanism is positioned at a smear position and used for smearing a to-be-processed sample on the glass slide when the conveying mechanism conveys the glass slide to the smear position;

the turnover mechanism is positioned at the turnover position and used for turning over the glass slide to a preset angle when the conveying mechanism conveys the glass slide to the turnover position;

the transfer mechanism is controllably moved to a turning position, the turning position, a dip-dyeing position and an air drying position are sequentially distributed along the movement track of the transfer mechanism, the dip-dyeing position is used for placing a dyeing container containing a dyeing reagent, the transfer mechanism is used for transferring the glass slide from the turning position to the dyeing container for dyeing, and is used for transferring the glass slide from the dyeing container to the air drying position for air drying to prepare a sheet of sample; wherein, the sample to be processed is a semen sample.

In one embodiment, the transfer mechanism comprises a conveying mechanism, the sheet making mechanism comprises a smear mechanism, and the sheet making position comprises a coating position and an air drying position;

the conveying mechanism controllably moves, the sample adding position, the smear position and the air drying position are sequentially distributed along the movement track of the conveying mechanism, and the conveying mechanism is used for bearing and conveying glass slides;

the to-be-processed sample is formed by mixing a semen sample and a reaction reagent, and when the conveying mechanism conveys the glass slide to the air drying position, the to-be-processed sample on the glass slide is air-dried and then is made into a sheet sample.

In one embodiment, the transfer mechanism comprises a conveying mechanism and a transfer mechanism, the film making mechanism comprises a diffusion mechanism and a refrigeration mechanism, and the film making positions comprise a diffusion position, a refrigeration position, a dip-dyeing position and an air drying position;

the conveying mechanism controllably moves, the sample adding position, the diffusion position and the refrigeration position are sequentially distributed along the movement track of the conveying mechanism, and the conveying mechanism is used for bearing and conveying glass slides;

the diffusion mechanism is positioned at the diffusion position and used for adding a diffusion tool to the glass slide when the conveying mechanism conveys the glass slide to the diffusion position so as to diffuse the sample to be processed on the glass slide;

the refrigerating mechanism is positioned at a refrigerating position and used for refrigerating and preserving the glass slide when the conveying mechanism conveys the glass slide to the refrigerating position so as to separate the diffusion tool from the glass slide;

the transfer mechanism is controllably moved to a cold storage position, the cold storage position, a dip dyeing position and an air drying position are sequentially distributed along the movement track of the transfer mechanism, the dip dyeing position is used for placing a dyeing container containing a dyeing reagent, the transfer mechanism is used for transferring the glass slide from the cold storage position to the dyeing container for dyeing, and is used for transferring the glass slide from the dyeing container to the air drying position for air drying to prepare a piece of sample;

wherein, the sample to be processed is formed by mixing a semen sample and a reaction reagent.

In one embodiment, the sample application mechanism comprises:

the sampling pipeline is used for collecting a sample to be processed;

the sampling driving part is connected with the sampling pipeline and is used for driving the sampling pipeline to collect a sample to be processed;

the sampling pipeline is arranged on the sampling walking driving part, and the sampling walking driving part is used for driving the sampling pipeline to move; and

and the viscosity detector is arranged on the sampling pipeline and is used for detecting the viscosity of the sample to be processed.

In one embodiment, the slide glass loading device further comprises a slide loading mechanism, the movement track of the transfer mechanism further defines a slide loading position, the sample loading position and the slide making position are sequentially distributed along the movement track of the transfer mechanism, and the slide loading mechanism is used for adding a slide glass to the transfer mechanism from the slide loading position.

In one embodiment, the slide glass slide.

In one embodiment, the device further comprises a sample feeding mechanism, wherein the sample feeding mechanism comprises a sample feeding conveying part and an incubation part, and a sample feeding position is defined along the motion track of the sample feeding mechanism;

the sample injection conveying piece is used for bearing and conveying a sample container containing a semen sample so that the sample injection mechanism collects the semen sample from the sample container positioned at a sample injection position;

the incubator is mounted on a sample transport for heating the sample container to maintain a temperature of a semen sample within the sample container within a preset range.

In one embodiment, the semen collection device further comprises a scanning mechanism, wherein a scanning position is defined along the conveying direction of the sample conveying member, and the scanning mechanism is located at the scanning position and used for scanning information of the sample container when the sample conveying member conveys the sample container to the scanning position so as to obtain information of the semen sample in the sample container.

In one embodiment, the device further comprises a reaction mechanism for containing the semen sample and the reaction reagent, wherein a sampling position is defined on the movement track of the sample adding mechanism, and the sampling position and the sample adding position are sequentially distributed along the movement track of the sample adding mechanism;

the reaction mechanism is positioned at a sampling position, so that the sample adding mechanism collects a sample to be processed from the reaction mechanism and adds the sample to be processed to the glass slide; wherein, the sample to be processed is formed by mixing a semen sample and a reaction reagent.

The semen sample slide-making device according to the embodiment comprises a sample adding mechanism, a slide-making mechanism and a transfer mechanism for transferring and transporting a slide; wherein, there are application of sample position and film-making position along the movement track definition of transport mechanism, application of sample mechanism moves to application of sample position in order to add pending sample on the slide glass in a controlled manner, and film-making mechanism then is located film-making position to the pending sample to on the slide glass carries out the film-making, thereby makes into a piece sample. Through the cooperation between each mechanism, can accomplish sample collection, application of sample and film-making action automatically in succession, realize automatic, the batchization film-making to the seminal fluid sample, be favorable to improving film-making efficiency and guaranteeing the quality of piece sample to the produced series of problems of manual film-making have been solved.

Drawings

FIG. 1 is a schematic view (one) of the functional site distribution of the semen sample preparation device in the first embodiment;

FIG. 2 is a schematic view (two) showing the distribution of functional sites of the semen sample preparing apparatus according to the first embodiment;

FIG. 3 is a schematic view (one) of the functional site distribution of the semen sample preparation device in the second embodiment;

FIG. 4 is a schematic view (II) of the function site distribution of the semen sample preparation device in the second embodiment;

FIG. 5 is a schematic view (one) of the function site distribution of the semen sample preparation device in the third embodiment;

FIG. 6 is a schematic view (two) showing the distribution of functional sites of the semen sample preparing apparatus according to the third embodiment;

FIG. 7 is a schematic view (one) of the functional site distribution of the semen sample preparation device in the fourth embodiment;

FIG. 8 is a diagram showing the distribution of functional sites of the semen sample preparation apparatus in the fourth embodiment (II);

FIG. 9 is a schematic diagram showing a structural layout of the mechanisms of the semen sample producing apparatus according to an embodiment;

FIG. 10 is a schematic view of the sample injection mechanism in FIG. 9;

FIG. 11 is a schematic view of a structural reference of the loading mechanism of FIG. 9;

FIG. 12 is a schematic view of a functional principle of a system of a sperm cell morphometer in accordance with a fifth embodiment;

in the figure:

a. sample adding position; b. coating a chip position; c. turning over the bits; d. sampling bits; e. dip dyeing sites; f. air-drying the blank; g. a loading position; h. printing a bit; k. sampling position; m, scanning position; n, a diffusion bit; p, a refrigeration position;

A. a dyeing vessel; B. cleaning the container; D. a sample container;

100. a sample adding mechanism; 110. a sampling drive member; 120. a viscosity detector; 130. a sampling needle;

200. a conveying mechanism; 210. a first conveying unit; 220. a second conveying unit; 230. a third conveying unit;

300. a transfer mechanism; 310. grabbing the functional piece; 400. a smear mechanism; 500. a turnover mechanism;

600. a sheet feeding mechanism; 610. a tablet storage bin; 620. a feeding driving member; 621. a screw motor; 622. positioning seats; 623. a pusher dog; 624. an upper stop pin; 625. a lower stop pin; 626. a loading support; 627. limiting the guide rail; 628. a rotating shaft;

700. a printing mechanism; 800. a sample introduction mechanism; 810. a sample introduction conveying member; 820. an incubation member; 900. a scanning mechanism; 1000. a reaction mechanism; 1100. a diffusion mechanism; 1200. a refrigeration mechanism; 1300. and (4) reading the film.

Detailed Description

The present invention will be described in further detail with reference to the following detailed description and accompanying drawings. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).

The semen sample slide device is mainly used for slide dyeing of semen samples, provides a slide sample for subsequent detection and analysis items such as sperm survival rate, sperm morphology, sperm DNA fragmentation degree and the like, and is provided with a transfer mechanism, a sample adding mechanism and a slide mechanism; wherein: the transfer mechanism controllably moves, and a plurality of functional positions are defined along the movement track of the transfer mechanism, wherein the functional positions comprise a sample adding position and a slide preparing position and are used for conveying the slide to the sample adding position and the slide preparing position; the sample adding mechanism controllably moves to a sample adding position and is used for adding a sample to be processed to the glass slide when the glass slide is conveyed to the sample adding position; the slide making mechanism is positioned at the slide making position and used for making the slide to be processed on the slide into a finished sample which can meet the detection and analysis requirements when the slide is conveyed to the slide making position; with this, through the cooperation between each mechanism, can accomplish sample collection, application of sample and film-making action automatically in succession, realize automatic, the batchization film-making to the seminal fluid sample, be favorable to improving film-making efficiency and guaranteeing the quality of film-making sample to the produced series of problems of manual film-making have been solved.

The semen sample film-making device of this application can be directed against semen sample detection analysis project, and adaptability or pertinence accomplish corresponding film-making action, if: only one item of the detection and analysis items such as the sperm survival rate, the sperm morphology, the sperm DNA fragmentation degree and the like is provided with the single function of manufacturing a corresponding piece sample to form a special piece manufacturing device; and the multifunctional slide making device can also have multiple functions of making corresponding slide samples aiming at two or all items in the detection and analysis items such as sperm survival rate, sperm morphology, sperm DNA fragmentation degree and the like, thereby forming the multifunctional slide making device.

Example one

Referring to fig. 1, fig. 2, fig. 9, fig. 10 and fig. 11 and with reference to fig. 12, the present embodiment provides a sperm sample slide making apparatus, which is mainly used for performing automatic batch slide making for sperm morphology detection and analysis items, and includes a sample adding mechanism 100, a transporting mechanism formed by or matching with a transporting mechanism 200 and a transferring mechanism 300, a slide making mechanism formed by or matching with a smear mechanism 400 and a turnover mechanism 500, and a control system for controlling the sample adding mechanism 100, the transporting mechanism 200, the transferring mechanism 300, the smear mechanism 400 and the turnover mechanism 500 to perform respective functional actions. Wherein:

the conveying mechanism 200 is mainly used for bearing and conveying glass slides, the conveying mechanism 200 controllably moves, a plurality of functional positions are defined along the movement track of the conveying mechanism, the plurality of functional positions comprise a sample adding position a and a slide making position consisting of a smear position b and a turnover position c, and the sample adding position a, the smear position b and the turnover position c are sequentially distributed along the movement track of the conveying mechanism 200; based on the different structural configurations and the remote selection of the conveying mechanism 200, the distribution of the functions on the conveying mechanism 200 is different, such as: referring to fig. 2, when the conveying mechanism 200 is a conventional carousel conveying mechanism or a rotary ring conveying mechanism, the conveying mechanism 200 is controllably rotated around its axis, and at this time, the sample loading position a, the smear position b, and the turn position c are sequentially distributed along the circumferential direction of the conveying mechanism 200. Referring to fig. 1, when the conveying mechanism 200 is a conventional linear conveying mechanism, the conveying mechanism 200 can move along the conveying direction, and at this time, the sample loading position a, the smear position b, and the turning position c are sequentially distributed along the conveying direction of the conveying mechanism. Thus, the conveying mechanism 200 can be used to sequentially convey the slide or the slide with the sample to be processed added to different functional positions to complete corresponding actions at the corresponding functional positions.

The smear mechanism 400 is mainly used for smearing the samples to be processed on the glass slide so that the samples to be processed are evenly smeared on the glass slide; the smear mechanism 400 is arranged at a smear position b, and when the conveying mechanism 200 conveys the slide glass added with the sample to be processed to the smear position b, the smear mechanism 400 executes automatic smear operation; specifically, when the conveying mechanism 200 adopts a turntable type conveying mechanism or a rotary ring type conveying mechanism, the smear mechanism 400 may be arranged on the periphery side of the conveying mechanism 200 or erected on the surface side of the conveying mechanism 200; when the conveying mechanism 200 adopts a linear conveying mechanism, the smear mechanism 400 can be arranged at the side of the conveying mechanism 200 or erected on the surface side of the conveying mechanism 200; by repeatedly pushing and pulling the smear sheet at a predetermined angle, it is possible to uniformly smear the specimen to be processed on the slide glass.

The inverting mechanism 500 is mainly used to invert the slide to a preset angle so that the transfer mechanism 300 can transfer the slide out of the conveying mechanism 200 in a manner such as grabbing; the turnover mechanism 500 is arranged at the turnover position c, and when the conveying mechanism 200 conveys the smeared slide glass to the turnover position c, the turnover mechanism 500 executes the slide glass turnover operation; the layout between the turnover mechanism 500 and the conveying mechanism 200 can be set by referring to the layout between the smear mechanism 400 and the conveying mechanism 200; specifically, one end or one side of the slide is gradually jacked up by using the turnover mechanism 500, so that the one end or one side of the slide is gradually separated from the surface of the conveying mechanism 200, the slide is turned over to a preset angle, and then the subsequent transfer action can be performed by the transfer mechanism 300 by grabbing one end or one side of the slide.

Referring to fig. 10, the sample adding mechanism 100 mainly includes a sampling traveling driving member, a sampling driving member 110, a viscosity detector 120, and a sampling pipeline mainly formed by connecting a sampling tube and a sampling needle 130; the sampling driving member 120 is connected to the sampling needle 130 through a sampling tube to drive the sampling needle 130 to collect semen samples, and the semen samples are uniformly mixed by repeatedly sucking and spitting the semen samples, so as to finally form a to-be-processed sample. Sampling pipeline installs on sampling walking driving piece, and sampling walking driving piece can drive sampling pipeline and move, then defines sampling position d on sampling pipeline's motion trail, and sampling position d distributes along sampling pipeline's motion trail in proper order with application of sample position an to utilize sampling driving piece to drive sampling needle 130 and can remove between sampling position d and application of sample position an, in order to accomplish sampling and application of sample function. The viscosity detector 120 is installed on the sampling pipeline for detecting the viscosity of the sample to be processed, and if the viscosity detector employs a pressure sensor, the viscosity detector may be installed at one end of the sampling needle 130 or the sampling pipeline away from the sampling driving member 110, and the viscosity of the sample to be processed is determined by detecting the internal pressure change of the sampling pipeline. In this embodiment, the sampling walking driving member can adopt existing devices such as a manipulator, a gantry truss, and the like according to actual conditions, so that the sample adding mechanism 100 has the characteristic of moving in at least one direction or one dimension, and simultaneously has the characteristic of moving along the X-axis direction, the Y-axis direction, and the Z-axis direction.

The transfer mechanism 300 is mainly used for transferring the slide from the conveying mechanism 200 to other functional positions so as to complete the treatments of dyeing, air drying and the like of the sample to be treated on the slide at the corresponding functional position; the transfer mechanism 300 comprises a gripping function part 310 (such as an electric claw, a mechanical arm and the like) and a transfer walking driving part, wherein the gripping function part 310 is installed on the transfer walking driving part, the transfer walking driving part can drive the gripping function part 310 to move, a slide making position consisting of a dip-dyeing position e and an air drying position f is defined on the movement track of the gripping function part 310, and the turning position c, the dip-dyeing position e and the air drying position f are sequentially distributed along the movement track of the gripping function part 310, so that the transfer walking driving part is used for driving the gripping function part 310 to move among the turning position c, the dip-dyeing position e and the air drying position f to complete the action of transferring the slide glass to the related function position; the staining container A containing staining reagents and the cleaning container B containing cleaning liquids are placed at the staining site e, and for the sperm morphology detection analysis items, the staining reagents suitable for a Papanicolaou staining method, a Shaorr staining method (i.e. a shorr staining method) or a Diff-Quick staining method (i.e. a Diff-Quick staining method) can be injected into the staining container A in advance, or a plurality of staining sites e are arranged on the motion track of the grabbing functional part 310 so as to place the cleaning container B or a plurality of staining containers A containing different staining reagents, so that the transfer mechanism 300 transfers the slide from the overturning site c into the staining container A for staining, and transfers the slide into the cleaning container B after staining to clean the residual staining reagents on the slide; and then, the glass slide is transferred to an air drying position f for air drying treatment, so that a piece of sample is finally prepared. In this embodiment, a supporting device such as a rack that can carry the slide glass may be disposed at the air drying position f to provide a placing space for the slide glass after the staining treatment; meanwhile, after the slide glass is transferred to the air drying position f by the transfer mechanism 300, the slide glass can be air-dried in a natural air drying manner, and the sample to be processed on the slide glass can also be air-dried forcibly by arranging an exhaust system such as a fan at the air drying position f. In one embodiment, the transfer travel drive can be configured with reference to the sampling travel drive. In another embodiment, the transferring mechanism 300 may also be composed of a plurality of grabbing function members 310 and a plurality of corresponding transferring and traveling driving members, especially in the case that the turning position c, the dyeing position e and the air drying position f are arranged in different directions, or the space of the area where the dyeing position e is located is large, and the transferring mechanism 300 needs to have a multi-dimensional or multi-directional movement function.

Meanwhile, it should be noted that the sample loading position a, the smear position b and the turning position c are sequentially distributed along the conveying direction of the conveying mechanism, and the turning position c, the dip dyeing position e and the air drying position f are sequentially distributed along the movement track of the grabbing kinetic energy part 310, wherein the sequential distribution can be structural layout significance or sequential arrangement in physical structure, and can also be action time sequence significance or sequential arrangement in control time sequence significance.

Based on this, utilize the characteristics of function mechanism such as application of sample mechanism 100, conveying mechanism 200 and transfer mechanism 300 can walk or movable, through overall arrangement and distribution to each function position, with application of sample mechanism 100, conveying mechanism 200, transfer mechanism 300, smear mechanism 400 and tilting mechanism 500 etc. link up the cooperation, can accomplish sample collection in succession automatically, application of sample and film-making action, realize the automation to the semen sample, the batch film-making operation, be favorable to improving film-making efficiency and guaranteeing the quality of piece sample, and solved the produced serial problems such as film-making inefficiency of manual film-making, intensity of labour is big, the unable standard quantization of piece quality.

In one embodiment, referring to fig. 1, fig. 2, fig. 9 and fig. 11, the semen sample slide making apparatus further includes a slide loading mechanism 600, a slide loading position g is defined on a movement track of the conveying mechanism 200, the slide loading position g, a sample loading position a, a smear position b and a flipping position c are sequentially distributed along the movement track of the conveying mechanism 200, the slide loading mechanism 600 is fixedly aligned or movably aligned with the slide loading position g, and is mainly used for adding slides from the slide loading position g to the conveying mechanism 200, so that the entire slide making apparatus has a function of automatically adding slides, and creates advantages for improving slide making efficiency and automation degree by reducing manual operation steps to the maximum extent, and the slide loading mechanism 600 includes a slide storage bin 610 and a slide loading driving member 620; among them, the magazine 610 is selectively disposed on the peripheral side or the side of the conveying mechanism 200, depending on the structural configuration and the movement of the conveying mechanism 200, and is mainly used for storing and accommodating unused slide glasses. The upper piece driving part 620 mainly comprises a screw rod motor 621, a positioning seat 622, a pusher dog 623, an upper stop pin 624, a lower stop pin 625, an upper piece bracket 626 and a limit guide rail 627; wherein:

the tablet storage bin 610 is mounted on an upper tablet support 626, a tablet discharging channel is arranged at the bottom of the tablet storage bin 610, a body of a lead screw motor 621 is mounted on the upper tablet support 626, a limiting guide rail 627 is mounted on the upper tablet support 626 along a second direction and located on the bottom end side of the tablet storage bin 610, a positioning seat 622 is in threaded connection with a power shaft of the lead screw motor 621 and is in sliding connection with the limiting guide rail 627 at the same time, a pusher dog 623 is rotatably arranged on the positioning seat 622 through a rotating shaft 628, the gravity center of the pusher dog 623 is located at the lower end of the pusher dog 623, an upper limiting pin 624 is mounted on the positioning seat 622 and located on the upper end side of the pusher dog 623 in the tablet discharging direction, and a lower limiting pin 625 is mounted on the positioning seat 622 and located on the lower end side of the pusher dog 623 in the direction opposite to the tablet discharging direction. In the initial state, the finger 623 rotates around the rotating shaft 628 under the action of gravity and stops after contacting the lower stop pin 625, and at the moment, the top end of the finger 623 extends into the slide storage bin 610 through the slide outlet channel so as to abut against one side of the slide far away from the slide loading position g; when the screw motor 621 drives the positioning seat 622 to move towards the side of the upper slide position g, the pusher dog 623 can synchronously drive the slide to move, so that the slide is finally pushed out of the slide storage bin 610 and enters the conveying mechanism 200 at the upper slide position g; when the positioning seat 622 is reset by the screw motor 621, the pushing action of the slide glass in the slide storage bin 610 can enable the pusher dog 623 to rotate around the rotating shaft 623, so that the top end of the pusher dog 623 is moved out of the slide storage bin 610 and further abutted against the upper stop pin 624 until the pusher dog returns to the original position, and after the pusher dog 623 is disengaged from the pushing action of the slide glass, the pusher dog can rotate under the action of gravity again, so that the top end of the pusher dog enters the slide storage bin 610 through the sheet discharge so as to abut against one side of the next slide glass, which is far away from the sheet loading position g; by cycling this way, slides can be added to the transport mechanism 200 continuously.

In one embodiment, two loading positions g can be defined along the movement track of the conveying mechanism 200, and two loading mechanisms 600 are configured to make the two loading mechanisms 600 stand by, which can effectively avoid the problem that the apparatus needs to be stopped due to the failure, the lack of slides, the supplement of slides, etc. of the loading mechanism 600 compared with the configuration of one loading mechanism 600, and create conditions for improving the operation efficiency of the apparatus.

In one embodiment, referring to fig. 1, fig. 2 and fig. 9, the semen sample preparing device further includes a printing mechanism 700, and a printing position h is further defined on the motion track of the conveying mechanism 200; the loading position g, the loading position a, the smear position b and the overturning position c can be sequentially distributed along the motion track of the conveying mechanism 200, and the loading position g, the loading position a, the smear position b, the overturning position c and the printing position h can also be sequentially distributed along the motion track of the conveying mechanism 200; the printing mechanism 700 is disposed at the printing position h, so that when the conveying mechanism 200 conveys the slide from the loading position g to the printing position h, the slide-making information (such as what kind of detection and analysis items the sample subsequently carried by the slide is suitable for, the attribute information of the sample, and the like) is printed on the slide by fixedly aligning the slide or movably aligning the slide, thereby facilitating the detection and analysis of the sample at the later stage, such as the sperm morphology analysis, and providing data or signal support for the control system to control the matching action of the related function mechanism. In this embodiment, the printing mechanism 700 can be implemented by a conventional slide printing device, such as a laser printing device, and the like, which will not be described herein.

In one embodiment, referring to fig. 1, fig. 2 and fig. 9, the semen sample slide apparatus further includes a sample injection mechanism 800 for providing the semen sample for the sample injection mechanism 100 to sample, wherein the sample injection mechanism 800 includes a sample injection conveying member 810 and an incubation member 820; wherein:

a sample injection site k is further defined along the movement track of the sample injection mechanism 100, the sample injection site k can be identical to the sample injection site D (i.e., in this embodiment, it can be understood that the two functional sites are the same position), and the sample injection transport element 810 is mainly used for carrying and transporting the sample container D containing the semen sample, so that the sample injection mechanism 100 can suck or collect the semen sample from the sample container D when the sample container D passes through and stays at the sample injection site k. In this embodiment, the sample transport member 810 may be a linear transport device (e.g., a belt transport device) reciprocating in a certain direction, or a circulating transport device capable of rotating clockwise and/or counterclockwise around a certain axis, and mainly enables the sample container D to pass through and stay at the sample injection position k. The incubation member 820 is disposed on the sample feeding member 810 and is used for heating the sample container D, so that the temperature of the semen sample in the sample container D can be maintained within a preset range (about 37 ℃), the semen sample can be conveniently transformed from a solidification state to a liquefaction state, and the semen sample to be detected is liquefied as soon as possible. In this embodiment, the incubation member 820 is a heating film attached to the side of the sample transport member 810 near the sample container D.

In one embodiment, referring to fig. 1, fig. 2 and fig. 9, the semen sample preparing apparatus further includes a scanning mechanism 900 for performing information scanning on the sample container D to obtain attribute information of the semen sample in the sample container D, a scanning position m is defined along the conveying direction of the sample conveying member 810, and the scanning mechanism 900 is disposed at the scanning position m; because semen samples contained in each sample container D have respective attributes (such as belonging to different persons), a corresponding information tag (such as a two-dimensional code, a bar code, etc.) is usually set on the sample container D, and at this time, when the sample transport element 810 transports (or simultaneously stops) the sample container D to the scanning position m, the scanning mechanism 900 can be movably aligned or fixedly aligned with the sample container D, so as to perform information scanning to confirm and record the information of the semen sample; in this way, not only can the recording and confirmation of the information of the semen sample be automatically realized, but also the printing mechanism 700 and the scanning mechanism 900 in the foregoing embodiment can be controlled in a correlated manner by the control system, so that the slide and the semen sample correspond to each other one by one. In this embodiment, the scanning mechanism 900 may employ a conventional information scanning gun, camera, or the like.

In addition, in order to avoid cross contamination between samples and reagents, a cleaning mechanism capable of cleaning the inner and outer walls of the sample adding mechanism 100 (especially the sampling needle 130 and the sampling tube) can be configured at the same time, or a cleaning position is arranged on the motion trail of the sample adding mechanism 100, and a relevant container containing cleaning liquid is arranged at the cleaning position, so that the sample adding mechanism 100 is cleaned firstly before collecting semen samples and relevant reagents; the specific structure or layout of the cleaning mechanism or the cleaning position can be selected and set according to the actual situation, which is not described herein. Meanwhile, the semen sample slide device can be operated under the control of the control system when in operation, the control system can be a control system of the semen sample slide device and can also be a control system used for controlling the semen sample slide device, according to the actual situation, the existing functional devices such as a computer, a single chip microcomputer, a PLC controller and the like, or the existing distributed or centralized control system can be adopted to complete the coordination management and control of each functional mechanism in the sperm sample production device by using the control system, including but not limited to the start-stop control of each mechanism, the receiving and feedback of signals and the like, and the control system has the function of indirectly controlling or directly controlling each functional mechanism to execute corresponding operation actions, the functionality may be implemented based on software algorithms within the implanted control system, the system functional architecture, or a combination of both.

Taking the sperm morphology slide-making process for a sperm sample as an example, the sperm sample slide-making device can execute the operation tasks by referring to the following processes, specifically:

step 11, after the sample container D is placed on the sample transport element 810, the semen sample in the sample container D may be heated by the incubation element 820, when the sample transport element 810 transports the sequential sample containers D to the scanning position m, the scanning mechanism 900 scans the semen sample sequentially to confirm and record the semen sample, and at the same time, starts to record the time for heating the semen sample (i.e., the liquefaction time), and after the liquefaction time of the semen sample reaches the preset time (e.g., the liquefaction time may be divided into a plurality of time periods such as 5min and 20 min), the sample adding mechanism 100 collects the semen sample from the sample container D located at the sample injection position k (i.e., the sampling position D), and then step 12 is executed.

Step 12, detecting whether the collected semen sample is completely liquefied by using the viscosity detector 120, and if so, executing step 13; if not, the semen sample is returned to the sample container D and step 11 is executed, or the semen sample is transferred to a container containing a diluent (of course, the container may be arranged on the moving track of the sample adding mechanism 100) and diluted until the semen sample is completely liquefied.

Step 13, the sample adding mechanism 100 moves to the sample adding position a, adds the semen sample to the slide, and executes step 15.

Step 14, while executing step 11 or step 12, the loading mechanism 600 adds the slide from the loading position g to the conveying mechanism 200, and when the conveying mechanism 200 conveys the slide to the printing position h, the printing mechanism 700 executes the information printing action, and then the conveying mechanism 200 conveys the slide to the loading position a.

Step 15, the conveying mechanism 200 conveys the slide to the smear position b, the smear mechanism 400 performs the smear action, and then step 16 is performed.

Step 16, the conveying mechanism 200 conveys the slide to the turning position c, the turning mechanism 500 turns the slide to a preset angle, the transfer mechanism 300 grabs the slide from the turning position c, and then step 17 is executed.

Step 17, the transfer mechanism 300 transfers the slide glass to the staining container a and the cleaning container B located at the dip-staining position e in sequence, so as to complete the staining treatment of the sperm sample, and then step 18 is performed.

Step 18, the transfer mechanism 300 transfers the slide to the air-drying position f to finish the natural air-drying or forced air-drying of the semen sample at the air-drying position f, so that the finished sample can be made.

Example two

Referring to fig. 3, fig. 4, fig. 9, fig. 10 and fig. 11 in combination with fig. 12, the present embodiment provides a semen sample preparing apparatus, which is different from the first embodiment in that: the transfer mechanism includes the conveying mechanism 200, but the transfer mechanism 300 is omitted; the film making mechanism comprises a film smearing mechanism 400, but the turnover mechanism 500 is omitted; correspondingly, a sample adding position a, a smear position b and an air drying position g are defined along the motion track of the conveying mechanism 200, so that the semen sample slide making device is mainly used for making automatic and batch slide making aiming at the sperm motility rate detection analysis item.

In this embodiment, the sample adding mechanism 100 collects a to-be-processed sample formed by uniformly mixing a semen sample and a viability stain from a sampling position d, adds the to-be-processed sample to a slide located at a sample adding position a, then the conveying mechanism 200 conveys the slide to a smear position b, the smear mechanism 400 smears the to-be-processed sample on the slide, and finally the conveying mechanism 200 conveys the slide to an air drying position g for natural air drying or forced air drying so as to finally prepare a sheet sample.

In an embodiment, referring to fig. 3, fig. 4 and fig. 9, the semen sample slide-making apparatus further includes a reaction mechanism 1000, which is mainly used for containing the semen sample and a reaction reagent such as a viability stain, and the reaction mechanism 1000 is disposed at the sampling site d (note that, in the case where the sampling site k in the first embodiment exists at the same time, the two sampling sites belong to different functional sites); so that application of sample mechanism 100 mixes in adding to reaction mechanism 1000 through gathering behind semen sample and the reaction reagent respectively, and based on application of sample mechanism 100's structure and functional structure, through repeatedly inhaling the mixture of spitting semen sample and rate of activity coloring agent, fully mix both, thereby form the sample to be processed, and later add the sample to be processed to being located on the slide glass of application of sample position an again. In this embodiment, the reaction mechanism 1000 may be mainly composed of a reaction container to provide a space for accommodating the semen sample and the reaction reagent.

Taking a process of preparing a sperm motility rate slice for a sperm sample as an example, the sperm sample slice device can execute an operation task by referring to the following processes, specifically:

step 21, after the sample container D is placed on the sample transport element 810, the semen sample in the sample container D may be heated by the incubation element 820, when the sample transport element 810 transports the sequential sample containers D to the scanning position m, the scanning mechanism 900 scans the semen sample sequentially to confirm and record the semen sample, and at the same time, starts to record the time for heating the semen sample (i.e., the liquefaction time), and after the liquefaction time of the semen sample reaches the preset time (e.g., the liquefaction time may be divided into a plurality of time periods such as 5min and 20 min), the sample adding mechanism 100 collects the semen sample from the sample container D located at the sample injection position k (i.e., the sampling position D), and then performs step 22.

Step 22, detecting whether the collected semen sample is completely liquefied by using the viscosity detector 120, if so, executing step 23; if not, the semen sample is returned to the sample container D and the step 21 is executed, or the semen sample is transferred to the reaction mechanism 1000, and the sample is diluted by adding a diluent into the reaction mechanism 1000 until the semen sample is completely liquefied.

Step 23, moving the sample adding mechanism 100 to the sampling position d to add the semen sample into the reaction mechanism 1000, then collecting the viability stain and adding the viability stain into the reaction container 1000, mixing the semen sample and the viability stain uniformly, and finally executing the step 25.

Step 24, while executing step 21, step 22 or step 23, the loading mechanism 600 adds the slide from the loading position g to the transport mechanism 200, and when the transport mechanism 200 transports the slide to the printing position h, the printing mechanism 700 executes the information printing operation, and then the transport mechanism 200 transports the slide to the loading position a.

Step 25, the sample adding mechanism 100 moves to the sample adding position a and adds the sample to be processed on the slide, and step 26 is executed.

Step 26, the conveying mechanism 200 conveys the slide to the smear position b, the smear mechanism 400 performs the smear action, and then step 27 is performed.

And 27, conveying the slide to an air drying position f by the conveying mechanism 200 to finish natural air drying or forced air drying of the semen sample at the air drying position f, so that the finished sample can be prepared.

EXAMPLE III

Referring to fig. 5, fig. 6, fig. 9, fig. 10 and fig. 11 in combination with fig. 12, the present embodiment provides a semen sample slide preparing apparatus, which is different from the first embodiment in that the slide preparing mechanism includes a diffusion mechanism 1100 and a refrigeration mechanism 1200, and the slide preparing mechanism 400 and the turnover mechanism 500 are omitted; correspondingly, a sample adding position a, a diffusion position n and a cold storage position p which are sequentially distributed are defined along the movement track of the conveying mechanism 200, so that the semen sample slide making device is mainly used for automatically and massively making slides aiming at the sperm DNA fragmentation degree detection and analysis items.

In this embodiment, the sample adding mechanism 100 collects a sample to be processed, which is obtained by mixing a semen sample and a meltable gel, from the sampling position d, and adds the sample to be processed to a slide glass located at the sample adding position a. The diffusion mechanism 1100 is disposed at the diffusion position n, and is mainly used for transporting the slide from the sample loading position a to the diffusion position n by the transport mechanism 200, and adding a diffusion tool (such as a sheet-type diffusion tool) to the slide so that the diffusion tool can be stacked on the slide, and the sample to be processed on the slide is uniformly diffused on the slide by the gravity or siphon principle of the diffusion tool. The refrigerating mechanism 1200 is disposed at the refrigerating position p, and is mainly used for refrigerating and preserving a superposed structure of the slide glass and the diffusion tool (for example, refrigerating for 20-25min at 2-8 ℃), so that the diffusion tool can be separated from the slide glass, the transfer mechanism 300 can grab the slide glass, and sequentially transfer the slide glass into the staining container a and the cleaning container B at the staining position e for DNA fragment staining and cleaning, and finally transfer the slide glass to the air-drying position g for natural air-drying or forced air-drying, so as to finally make a sheet sample.

In this embodiment, the diffusion mechanism 1100 can be implemented using a related art device capable of adding diffusion tools to the slide in a stacked manner, such as by sucking the diffusion tools with a suction cup device and finally stacking the diffusion tools on the slide.

In an embodiment, referring to fig. 5, fig. 6 and fig. 9, the semen sample slide apparatus further includes a reaction mechanism 1000, which is mainly used for containing the semen sample and a reaction reagent such as fusible gel, the reaction mechanism 1000 is disposed at the sampling site d (note that, in the case where the sampling site k in the first embodiment exists at the same time, the two sampling sites belong to different functional sites); so that application of sample mechanism 100 mixes in adding mechanism 1000 through gathering semen sample and reaction reagent respectively after, and based on application of sample mechanism 100's structure and functional configuration, through repeatedly inhaling and spitting the mixture of semen sample and easily melt gel, fully mix both, thereby form the sample to be handled, and later add the sample to be handled to being located the slide glass of application of sample position a again. In this embodiment, the reaction mechanism 1000 may be mainly composed of a reaction container to provide a space for accommodating the semen sample and the reaction reagent.

Taking the sperm DNA fragmentation degree slide-making process for one semen sample as an example, the semen sample slide-making device can refer to the following processes to execute the operation tasks, specifically:

step 31, after the sample container D is placed on the sample transport element 810, the semen sample in the sample container D may be heated by the incubation element 820, when the sample transport element 810 transports the sequential sample containers D to the scanning position m, the scanning mechanism 900 scans the semen sample sequentially to confirm and record the semen sample, and at the same time, starts to record the time for heating the semen sample (i.e., the liquefaction time), and after the liquefaction time of the semen sample reaches the preset time (e.g., the liquefaction time may be divided into a plurality of time periods such as 5min and 20 min), the sample adding mechanism 100 collects the semen sample from the sample container D located at the sample injection position k (i.e., the sampling position D), and then performs step 32.

Step 32, detecting whether the collected semen sample is completely liquefied by using the viscosity detector 120, if so, executing step 33; if not, the semen sample is returned to the sample container D and the step 31 is executed, or the semen sample is transferred to the reaction mechanism 1000, and the sample is diluted by adding a diluent into the reaction mechanism 1000 until the semen sample is completely liquefied.

Step 33, moving the sample adding mechanism 100 to the sampling position d to add the semen sample into the reaction mechanism 1000, then collecting the easily meltable gel and adding the easily meltable gel into the reaction container 1000, mixing the semen sample and the easily meltable gel uniformly, and finally executing step 35.

Step 34, while executing step 31, step 32 or step 33, the loading mechanism 600 adds the slide from the loading position g to the transport mechanism 200, and when the transport mechanism 200 transports the slide to the printing position h, the printing mechanism 700 executes the information printing operation, and then the transport mechanism 200 transports the slide to the loading position a.

At step 35, the transport mechanism 200 transports the slide to the diffusion station n, and the diffusion mechanism 1100 performs the add-diffusion-tool action, followed by step 36.

Step 36, the conveying mechanism 200 conveys the slide and the diffusion tool to a refrigerating position p, the refrigerating mechanism 1200 performs refrigerating and preserving treatment on the slide and the diffusion tool, after the refrigerating and preserving are finished, the diffusion tool is separated from the slide by manual operation or configuration of a corresponding structure, the transfer mechanism 300 grabs the slide from the refrigerating position p, and then step 37 is executed.

In step 37, the transfer mechanism 300 sequentially transfers the slide to the staining container a and the washing container B located at the dip-staining position e, so as to complete the staining process on the sperm sample, and then step 38 is performed.

Example four

Referring to fig. 7, fig. 8, fig. 9, fig. 10 and fig. 11 in combination with fig. 12, the present embodiment provides a semen sample preparing apparatus, which is different from the first, second and third embodiments in that the transporting mechanism includes a conveying mechanism 200 and a transferring mechanism 300, and the preparing mechanism includes a smear mechanism 400, a turnover mechanism 500, a diffusion mechanism 1100 and a refrigerating mechanism 1200; correspondingly, a sample adding position a, a smear position b, a turning position c, a diffusion position n and a cold storage position p are simultaneously defined along the movement track of the conveying mechanism 200, and an air drying position f is simultaneously positioned on the movement tracks of the transfer mechanism 300 and the conveying mechanism 200, so that the semen sample slide making device has the function of automatically and massively making slides aiming at a plurality of detection and analysis items such as sperm motility rate, sperm morphology, sperm DNA fragmentation degree and the like.

In this embodiment, referring to fig. 7 and 10, the conveying mechanism 200 may be composed of a plurality of linear conveying units (e.g., belt conveyors), such as a first conveying unit 210, a second conveying unit 220, and a third conveying unit 230, which are disposed side by side along a certain direction; a loading position g, a printing position h, a loading position a, a smear position b and an air drying position g are sequentially defined in the conveying direction of the first conveying unit 210 so as to meet the requirement of sperm motility flaking; a loading position g, a printing position h, a loading position a, a smear position b and a turning position c are sequentially defined in the conveying direction of the second conveying unit 220 so as to meet the requirement of sperm morphology slide production; and a loading position g, a printing position h, a loading position a, a diffusion position n and a refrigerating position p are sequentially defined in the conveying direction of the third conveying unit 230 so as to meet the requirement of sperm DNA fragmentation production.

In one embodiment, referring to fig. 8, the conveying mechanism 200 may also adopt a turntable type or a rotating ring type conveying mechanism, and each functional mechanism is sequentially disposed on the periphery of the conveying mechanism 200, such as a loading position g, a printing position h, a loading position a, a smearing position b, a turning position c, a diffusion position n, a refrigerating position p, and an air drying position g are sequentially defined on the rotating track of the conveying mechanism 200 along the circumferential direction; the forward and reverse rotation timing sequence of the conveying mechanism 200 is controlled by the control system according to the slide production mode (for example, when sperm DNA fragmentation production is carried out, the conveying mechanism 200 can be controlled to directly rotate the loaded slide from the sample loading position a to the diffusion position n, so that the slide jumps from the coating position b and the overturning position c), the slide is conveyed to the corresponding functional position, and then the functional mechanism corresponding to the functional position executes corresponding action.

EXAMPLE five

Referring to fig. 1 to 12, the present embodiment provides a sperm morphology analyzer, which mainly includes a slide reading device 1300 and a semen sample slide making device according to any of the foregoing embodiments; the slide reading device 1300 is used for performing image scanning analysis on the slide samples made by the semen sample slide making device to obtain one of the information of the survival rate, the morphological information and the DNA fragmentation degree information of the semen samples on the slide samples; the semen sample slide device and the slide reading device can be connected through a conveying mechanism 200 or a transferring mechanism 300, so that after the slide sample is prepared, the slide sample is directly conveyed to the slide reading device 1300, a multifunctional sperm morphology analyzer integrating functions of automatic sampling, automatic sample adding, automatic slide preparation, automatic slide reading and the like is formed, and conditions are created for rapid and batch sperm detection and analysis. It should be noted that: the arrow connecting lines in fig. 12 represent only the positional relationship or the fitting relationship among the functional mechanisms, and do not indicate the control relationship, the operation timing relationship, and the like among the functional mechanisms.

The present invention has been described in terms of specific examples, which are provided to aid understanding of the invention and are not intended to be limiting. For a person skilled in the art to which the invention pertains, several simple deductions, modifications or substitutions may be made according to the idea of the invention.

Claims

1. A semen sample slide producing device, comprising:

2. The apparatus for preparing a semen sample according to claim 1, wherein the transporting means comprises a transporting means and a transferring means, the preparing means comprises a smear means and a turnover means, and the preparing means comprises a smear station, a turnover station, a dip-dye station and an air-drying station;

3. The apparatus for preparing a semen specimen according to claim 1, wherein the transporting means comprises a transporting means, the preparing means comprises a smear station and an air drying station;

4. The apparatus for preparing a semen sample according to claim 1, wherein the transporting means comprises a transporting means and a transferring means, the preparing means comprises a diffusing means and a refrigerating means, and the preparing station comprises a diffusing station, a refrigerating station, a dip-dyeing station and an air-drying station;

5. The semen sample production device according to any one of claims 1 to 4, wherein the sample application mechanism comprises:

the sampling pipeline is used for collecting a sample to be processed;

6. The semen sample production device according to any of claims 1 to 4, further comprising a loading mechanism, wherein the movement track of the transport mechanism further defines a loading position, and the loading position, the loading position and the production position are sequentially distributed along the movement track of the transport mechanism, and the loading mechanism is used for adding the slide from the loading position to the transport mechanism.

7. The apparatus for preparing a semen sample according to claim 6, further comprising a printing mechanism, wherein the movement track of the transporting mechanism further defines printing positions, the loading position, the printing position, the loading position and the slide preparing position are sequentially distributed along the movement track of the transporting mechanism, and the printing mechanism is located at the printing positions and is used for printing preparation information on the slide when the transporting mechanism transfers the slide to the printing positions.

8. The semen sample production device according to any of claims 1 to 4, further comprising a sample injection mechanism, wherein the sample injection mechanism comprises a sample injection transport member and an incubation member, and a sample injection position is defined along a movement track of the sample injection mechanism;

9. The apparatus for preparing a semen sample as claimed in claim 8, further comprising a scanning mechanism, wherein a scanning position is defined along the transporting direction of the sample transport member, and the scanning mechanism is located at the scanning position and is used for scanning information of the sample container to obtain the information of the semen sample in the sample container when the sample transport member transports the sample container to the scanning position.

10. The semen sample slide making device according to claim 3 or 4, further comprising a reaction mechanism for containing the semen sample and the reaction reagent, wherein the movement track of the sample adding mechanism defines a sampling site, and the sampling site and the sample adding site are sequentially distributed along the movement track of the sample adding mechanism;