CN113466477B - Sample pretreatment method of clinical automatic analyzer - Google Patents

Sample pretreatment method of clinical automatic analyzer Download PDF

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Publication number
CN113466477B
CN113466477B CN202110775680.4A CN202110775680A CN113466477B CN 113466477 B CN113466477 B CN 113466477B CN 202110775680 A CN202110775680 A CN 202110775680A CN 113466477 B CN113466477 B CN 113466477B
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reaction cup
sample
rotating
disc
manipulator
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CN113466477A (en
Inventor
宋家玉
张哲�
刘亚娟
申业明
李闯
吴壮
刘晓
李鹏飞
蔡克亚
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Autobio Labtec Instruments Zhengzhou Co Ltd
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Autobio Labtec Instruments Zhengzhou Co Ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/00722—Communications; Identification
    • G01N35/00732—Identification of carriers, materials or components in automatic analysers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/0092—Scheduling
    • G01N35/0095—Scheduling introducing urgent samples with priority, e.g. Short Turn Around Time Samples [STATS]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N2030/067—Preparation by reaction, e.g. derivatising the sample
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00346—Heating or cooling arrangements
    • G01N2035/00356—Holding samples at elevated temperature (incubation)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/00722—Communications; Identification
    • G01N35/00732—Identification of carriers, materials or components in automatic analysers
    • G01N2035/00742—Type of codes

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
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  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Robotics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

The invention discloses a sample pretreatment method of a clinical automatic analyzer, which adopts a pretreatment mechanism comprising a pretreatment part, a buffer incubation part, a reagent storage part, a mixing part, a magnetic washing separation cleaning part, a transfer manipulator and a pipetting needle, wherein n common reagents can be added to a sample (1), n common reagents, a magnetic bead reagent and an eluent are added to a sample (2) containing the precipitant and the magnetic bead reagent, and two groups of reagents are added to a sample (3), the first group of reagents is m common reagents, the precipitant is contained, and the second group of reagents is the n common reagents, the magnetic bead reagent and the eluent.

Description

Sample pretreatment method of clinical automatic analyzer
Technical Field
The invention relates to the technical field of mass spectrum detection equipment, in particular to a sample pretreatment method of a clinical automatic analyzer.
Background
The liquid chromatography tandem mass spectrometry (LC-MS/MS) can add additional analysis capability, and can accurately identify and quantify micro-compounds in complex sample matrixes such as cell and tissue lysate, blood, plasma, urine, oral liquid and the like, so that the liquid chromatography tandem mass spectrometry (LC-MS/MS) has more and more application in clinical application.
However, existing LC-MS/MS perform sample pretreatment mainly by manual operation of a professional laboratory staff. The requirements on the professional skills of operators are high, time and labor are consumed, and risks of poor consistency, cross contamination, biological safety and the like exist. Secondly, different targets are often matched with different reagents and pretreatment modes, including a protein precipitation method, a liquid-liquid extraction method, a solid-phase extraction method and the like. Different processing methods require different functional modules and different workflows, and it is difficult to achieve high throughput automated pretreatment of samples. Particularly for pre-processing where there is a different item interleave, it is more difficult to achieve high throughput automation. The above factors have prevented LC-MS/MS to some extent from developing and applying clinically.
Disclosure of Invention
In order to solve the problems, the invention provides a safe, efficient and widely-used sample pretreatment method of a clinical automatic analyzer, which concretely adopts the following technical scheme:
the invention relates to a sample pretreatment method of a clinical automatic analyzer, which comprises a sample injection unit, a sample processing unit and a detection unit which are sequentially connected, wherein the sample processing unit comprises:
the pretreatment part is provided with a first fixed disc, a first rotating disc is arranged above the first fixed disc, and the first rotating disc is used for driving the reaction cup to move along the circumferential direction;
the buffer incubation part is arranged at one side of the first fixed disc and is provided with a second fixed disc, a magnetic absorber is arranged on the second fixed disc, a second rotating disc is arranged above the second fixed disc, and the second rotating disc is used for driving the reaction cup to move along the annular direction;
the reagent storage part is arranged at one side of the first fixed disk and comprises a reagent disk provided with a plurality of groups of reagent placement positions, and each group of reagent placement positions comprises a plurality of containing holes which are sequentially arranged along the radius of the reagent disk;
the mixing part is arranged at one side of the first fixed disc and is used for placing the reaction cup and uniformly mixing the sample and the reagent in the reaction cup;
the magnetic washing separation cleaning part is arranged on one side of the first fixed disk and comprises a transfer manipulator, an oscillation mixer, a liquid suction needle and a cleaning liquid filling needle, wherein a magnetic suction cleaning position for placing a reaction cup is arranged on one side of the liquid suction needle and the cleaning liquid filling needle, and the overlapped part of the transfer manipulator and the first fixed disk is in separation rotation displacement;
the first manipulator is arranged on one side of the first fixed disc and is used for transferring the empty reaction cup to the first rotating disc, and the overlapping part of the first manipulator and the first fixed disc is an empty cup placing position;
the second manipulator is arranged between the first fixed disc and the mixing part and is used for realizing the transfer of the reaction cup between the first rotating disc and the mixing part, and the overlapping part of the second manipulator and the first fixed disc is the mixing rotation and displacement;
the third manipulator is arranged between the first fixed disc and the second fixed disc and is used for moving the reaction cup from the first rotating disc to the second rotating disc and moving the reaction cup out of the first rotating disc, the overlapped part of the third manipulator and the first fixed disc is a slow rotation shift, and the overlapped part of the third manipulator and the second fixed disc is a sample receiving position;
the first sample injection needle is arranged between the first fixed disc and the sample injection unit, and the overlapping part of the first sample injection needle and the first fixed disc is a sample filling position;
the double-channel reagent needle is arranged between the first fixed disc and the reagent storage part, and the overlapping part of the double-channel reagent needle and the first fixed disc is a reagent filling position;
the second sample injection needle is arranged between the second fixed disc and the detection unit, the overlapped part of the second sample injection needle and the second fixed disc is a sample injection position, and the magnetic absorber is arranged corresponding to the sample injection position;
for the case of adding n (n is more than or equal to 1) common reagents and containing precipitants and magnetic bead reagents into a sample, the sample pretreatment method comprises the following steps:
s1: the first manipulator takes an empty reaction cup and places the empty reaction cup in a first rotating disc of the pretreatment part, and at the moment, the reaction cup is positioned at the empty cup placing position;
s2: rotating the first rotating disc to enable the reaction cup to rotate to a sample filling position, and filling one sample in the sample filling unit into the reaction cup through the first sample injection needle;
s3: rotating the first rotating disc to enable the reaction cup to rotate to a reagent filling position, and filling a first common reagent on the reagent disc into the reaction cup through a double-channel reagent needle;
s4: rotating the first rotating disc to enable the reaction cup to be rotated to be uniformly mixed and shifted, transferring the reaction cup into the uniformly mixing part through the second manipulator to perform full oscillation, and transferring the reaction cup back to the first rotating disc through the second manipulator after the treatment is completed to perform heat preservation cultivation;
s5: sequentially carrying out S3-S4, repeating n times, and completing filling, shaking and mixing and heat preservation cultivation of other common reagents and reagents containing precipitants and magnetic beads;
s6: rotating the first rotating disc to enable the reaction cup to rotate to a slow-breeding rotation position, and rotating the reaction cup into the second rotating disc of the buffer incubation part through the third manipulator, wherein the reaction cup is positioned at the sample receiving position;
s7: rotating the second rotating disc to enable the reaction cup to rotate to a sample injection position, sucking the supernatant in the reaction cup by a second sample injection needle and transferring the supernatant to a detection unit;
s8: rotating the second rotating disc to enable the reaction cup to return to the sample receiving position, and discarding the reaction cup after sample injection by the third manipulator;
for the case of adding n (n is more than or equal to 1) common reagents, magnetic bead reagents and eluents into a sample, the sample pretreatment method comprises the following steps:
s1: the first manipulator takes an empty reaction cup and places the empty reaction cup in a first rotating disc of the pretreatment part, and at the moment, the reaction cup is positioned at the empty cup placing position;
s2: rotating the first rotating disc to enable the reaction cup to rotate to a sample filling position, and filling one sample in the sample filling unit into the reaction cup through the first sample injection needle;
s3: rotating the first rotating disc to enable the reaction cup to rotate to a reagent filling position, and filling a first common reagent on the reagent disc into the reaction cup through a double-channel reagent needle;
s4: rotating the first rotating disc to enable the reaction cup to be rotated to be uniformly mixed and shifted, transferring the reaction cup into the uniformly mixing part through the second manipulator to perform full oscillation, and transferring the reaction cup back to the first rotating disc through the second manipulator after the treatment is completed to perform heat preservation cultivation;
s5: sequentially carrying out S3-S4, repeating n times, and completing filling, vibration mixing and heat preservation cultivation of other common reagents and magnetic bead reagents;
s6: rotating the first rotating disc to enable the reaction cup to rotate to the separation rotation position, enabling the reaction cup to enter the magnetic washing separation cleaning part through the transfer manipulator to carry out magnetic washing separation cleaning, and then moving the reaction cup back to the first rotating disc;
s7: repeating S3-S4, filling eluent into the reaction cup, and carrying out shaking mixing and heat preservation cultivation;
s8: rotating the first rotating disc to enable the reaction cup to rotate to a slow-breeding rotation position, and rotating the reaction cup into the second rotating disc of the buffer incubation part through the third manipulator, wherein the reaction cup is positioned at the sample receiving position;
s9: rotating the second rotating disc to enable the reaction cup to rotate to a sample injection position, sucking the supernatant in the reaction cup by a second sample injection needle and transferring the supernatant to a detection unit;
s10: rotating the second rotating disc to enable the reaction cup to return to the sample receiving position, and discarding the reaction cup after sample injection by the third manipulator;
aiming at the condition that two groups of reagents are added into a sample, wherein the first group of reagents are m (m is more than or equal to 1) common reagents and contain precipitants, and the second group of reagents are n (n is more than or equal to 1) common reagents, magnetic bead reagents and eluents, the sample pretreatment method comprises the following steps:
s1: the first manipulator takes an empty reaction cup X and places the empty reaction cup X in a first rotating disc of the pretreatment part, and at the moment, the reaction cup X is positioned at the empty cup placing position;
s2: rotating the first rotating disc to enable the reaction cup X to be rotated to a sample filling position, and filling one sample in the sample filling unit into the reaction cup X through the first sample injection needle;
s3: rotating the first rotating disc to enable the reaction cup X to be rotated to a reagent filling position, and filling a first common reagent on the reagent disc into the reaction cup X through a double-channel reagent needle;
s4: rotating the first rotating disc to enable the reaction cup X to be rotated to be mixed and shifted, transferring the reaction cup X into the mixing part through the second manipulator to perform full oscillation, and transferring the reaction cup X back to the first rotating disc through the second manipulator after the treatment is completed to perform heat preservation cultivation;
s5: sequentially carrying out S3-S4, repeating m times to finish filling, shaking and mixing and heat preservation cultivation of other common reagents and reagents containing precipitants and magnetic beads;
s6: rotating the first rotating disc to enable the reaction cup X to rotate to a separation rotation position, enabling the reaction cup X to enter a magnetic suction cleaning position of a magnetic cleaning separation cleaning part through a transfer manipulator, sucking away liquid after magnetic suction in the reaction cup X through a liquid suction needle, and then enabling the transfer manipulator to move the reaction cup X back to the first rotating disc;
s7: rotating the first rotating disc to enable the reaction cup X to rotate to a slow-breeding rotation displacement, and sending the reaction cup X and magnetic beads in the reaction cup X out of the first rotating disc through a third manipulator;
s8: repeating S1, and placing a new empty sample cup Y on the first rotating disc at the empty cup placing position;
s9: rotating the first rotating disc to enable the empty reaction cup Y to rotate to a separation rotation position, enabling the reaction cup Y to enter a magnetic suction cleaning position of a magnetic cleaning separation cleaning part through a transfer manipulator, driving liquid temporarily stored in a liquid suction needle into the reaction cup Y, and then rotating the reaction cup Y back to the first rotating disc;
s10: sequentially carrying out S3-S4, repeating (n+1) times, and completing filling, vibration mixing and incubation of common reagents and magnetic bead reagents in the second group of reagents;
s11: rotating the first rotating disc to enable the reaction cup Y to rotate to the separation rotation displacement, enabling the reaction cup Y to enter the magnetic washing separation cleaning part through the transfer manipulator to carry out magnetic washing separation cleaning, and then moving the reaction cup Y back to the first rotating disc;
s12: repeating S3-S4, filling eluent into the reaction cup Y, and carrying out shaking mixing and heat preservation cultivation;
s13: rotating the first rotating disc to enable the reaction cup Y to rotate to a slow-breeding rotation position, and rotating the reaction cup Y into the second rotating disc of the buffer incubation part through the third manipulator, wherein the reaction cup Y is positioned at the sample receiving position;
s14: rotating the second rotating disc to enable the reaction cup Y to rotate to a sample injection position, sucking the supernatant in the reaction cup Y by a second sample injection needle, and transferring the supernatant to a detection unit;
s15: and rotating the second rotating disc to enable the reaction cup Y to return to the sample receiving position, and discarding the reaction cup Y after sample injection by the third manipulator.
And constant-temperature incubation belts are arranged on the first fixed disc and the second fixed disc.
An empty reaction cup supply unit is arranged on one side of the first manipulator.
And one side of the third manipulator is provided with a reaction cup recycling mechanism.
And needle washing mechanisms are arranged on one sides of the liquid sucking needle, the cleaning liquid filling needle, the first sample injection needle, the double-channel reagent needle and the second sample injection needle.
The sample injection unit comprises
A sample conveyor belt for conveying sample tubes;
the classifying and storing disc is arranged at one side of the sample conveyor belt and is used for classifying and storing the sample tubes;
the emergency sample injection disc is arranged at one side of the classification storage disc and is used for storing sample tubes;
the sample injection pipe rack is arranged at one side of the classification storage disc and used for storing sample pipes;
the recovery pipe rack is arranged at one side of the classification storage disc and used for storing recovered sample pipes;
a first sample tube manipulator disposed between the sorting tray and the sample conveyor belt for transferring sample tubes between the sorting tray and the sample conveyor belt;
the second sample tube manipulator is arranged between the classified storage disc and the emergency sample introduction disc and is used for transferring the sample tube from the emergency sample introduction disc to the classified storage disc;
and the third sample tube manipulator is arranged between the sorting storage disc and the sample injection tube frame as well as between the sample tube and the recovery tube frame and is used for transferring the sample tubes from the sample injection tube frame to the sorting storage disc and transferring the sample tubes from the sorting storage disc to the recovery tube frame.
The emergency sample introduction disc is characterized in that a first code scanner is arranged on one side of the emergency sample introduction disc, and a second code scanner is arranged near the sample introduction pipe frame and the recovery pipe frame.
The detection unit is a liquid chromatography tandem mass spectrometry device, an immunoassay device or a biochemical analysis device.
According to the sample pretreatment method of the clinical automatic analyzer, the supported pretreatment modules are in a modularized design, and the modules are mutually independent, so that the method is convenient to debug, wide in application, flexible and convenient; the consistent pretreatment module is matched with the magnetic bead reagent containing the separable target objects, so that different pretreatment flows can be freely selected according to different target objects; the incubation reaction disk adopts a round reaction cup position, the reaction time is set freely, the reagent filling times are not limited, and the incubation reaction disk comprises a magnetic separation function; and (3) adopting an independent cleaning module, and returning the incubation of the cleaning process to the incubation reaction plate. The invention can ensure a high-flux automatic mode and improve the detection efficiency of the target object.
Drawings
Fig. 1 is a schematic structural view of the present invention.
Detailed Description
The following describes embodiments of the present invention in detail with reference to the accompanying drawings, and the embodiments and specific working procedures of the present invention are given by implementing the present embodiment on the premise of the technical solution of the present invention, but the protection scope of the present invention is not limited to the following embodiments.
As shown in fig. 1, the sample pretreatment method of the clinical automatic analyzer according to the present invention comprises a sample introduction unit 100, a sample processing unit 200 and a detection unit 300.
The sample injection unit 100 comprises a sample injection pipe rack 101 for storing sample pipes containing samples to be tested, a recovery pipe rack 102 for storing recovery sample pipes, and an emergency sample injection disk 103 for storing sample pipes to be processed in emergency, wherein the sample injection pipe rack 101, the recovery pipe rack 102 and the sample injection pipe rack 103 are distributed around a classification storage disk 104, and the classification storage disk 104 is positioned at one end of a circulating sample conveyor belt 105; a first sample tube manipulator 106 is arranged between the sample conveyor belt 105 and the classification storage disk 104, a second sample tube manipulator 107 is arranged between the classification storage disk 104 and the emergency sample introduction disk 103, and a third sample tube manipulator 108 is arranged between the classification storage disk 104 and the sample introduction pipe rack 101 and between the classification storage disk 104 and the recovery pipe rack 102; further, in order to identify and record each sample tube, a first code scanner 109 is installed on one side of the emergency sample tray 103, and a second code scanner 110 is installed near the sample tube rack 101 and the recovery tube rack 102.
According to the detection time requirement, the sample tubes to be processed are respectively placed in the sample tube rack 101 or the emergency sample tray 103, the sample tubes are transferred to the classification storage tray 104 through the second sample tube manipulator 107 and the third sample tube manipulator 108 to be classified and stored according to a certain sequence, then the sample tubes to be detected on the classification storage tray 104 are transferred to the sample conveyor belt 105 through the first sample tube manipulator 106 to be prepared for detection, and the sample tubes returned from the subsequent system are still transferred to the classification storage tray 104 through the first sample tube manipulator 106, and then are transferred to the recovery tube rack 102 through the third sample tube manipulator 108.
The sample processing unit 200 is used for preprocessing samples, and can process the required samples sequentially, in parallel and in a staggered manner according to the experimental purposes, and comprises a preprocessing part 201, auxiliary units distributed around the preprocessing part, a manipulator, a pipetting needle and the like. Specifically, the pretreatment portion 201 includes a first fixed disk, a first rotating disk is disposed above the first fixed disk, and a plurality of accommodating holes for accommodating reaction cups are formed in the first rotating disk, and when the first rotating disk rotates, the reaction cups disposed thereon move along with the first rotating disk in a circumferential direction (can rotate clockwise or counterclockwise). An empty reaction cup supply unit 202 (a common accommodating box is optional) is arranged on one side of the pretreatment part 201, and a first manipulator 203 is arranged between the empty reaction cup supply unit 202 and the common accommodating box and is used for moving the empty reaction cup to a first rotating disc, and an overlapping position of the first manipulator 203 and the first fixed disc is an empty cup placing position C1. A first sampling needle 204 is installed between the pretreatment part 201 and the sample conveyor belt 105, the first sampling needle 204 is used for sucking the sample to be tested in the sample tube and injecting the sample into the reaction cup on the first rotating disk, and the overlapping part of the first sampling needle 204 and the first fixed disk is a sample filling position C2. The pretreatment part 201 is also provided with a reagent storage part on one side, and comprises a reagent tray 205 provided with a plurality of groups of reagent placement positions, each group of reagent placement positions comprises a plurality of containing holes which are sequentially arranged along the radius of the reagent tray 205, and common reagents and/or reagents containing magnetic beads can be placed according to experimental requirements, and the number and the positions of the common reagents and the reagents containing magnetic beads are arranged according to actual conditions; a dual-channel reagent needle 206 is installed between the reagent disk 205 and the pretreatment portion 201, and is used for injecting one or two reagents of the reagent disk 205 into a reaction cup on the first rotating disk, and the overlapping part of the reagent disk and the first fixed disk is a reagent filling position C3. A mixing part 207 is further arranged on one side of the pretreatment part 201, and the mixing part 207 is used for placing a reaction cup and mixing a sample and a reagent in the reaction cup; a second manipulator 208 is arranged between the mixing part 207 and the first fixed disk, and the overlapping part of the second manipulator and the first fixed disk is a mixing rotation shift C4; the second robot 208 is used to grasp the cuvette and put it into the mixing section 207 from the first rotary plate, or put it into the first rotary plate from the mixing section 207. A magnetic washing separation and cleaning part is further arranged on one side of the pretreatment part 201, and comprises a transfer manipulator 209, an oscillation mixer 210, a liquid suction needle 211, a cleaning liquid filling needle 212 and a magnetic suction cleaning position 213 for placing a reaction cup which are sequentially arranged; the magnetic attraction cleaning position 213 can be used for placing a magnet to achieve the magnetic attraction purpose, namely, the magnetic beads for adsorbing the magnetic bead reagent in the sample; the liquid suction needle 211 is used for sucking liquid except magnetic beads in the sample, so that the cleaning liquid filling needle 212 can clean the surface of the adsorbed magnetic beads, or is used for sucking and temporarily storing the sample for secondary sample injection; the overlapping position of the transfer manipulator 209 and the first fixed disk is a separation and rotation shift C5. The buffer incubation part 214 is located at one side of the pretreatment part 201, and comprises a second fixed disk and a second rotating disk above the second fixed disk, wherein a plurality of accommodating holes for accommodating reaction cups are formed in the second rotating disk, and when the second rotating disk rotates, the reaction cups placed on the second rotating disk move along the annular direction. The third manipulator 215 is located between the first fixed disk and the second fixed disk, and is configured to move the reaction cup from the first rotating disk to the second rotating disk, or move the reaction cup on the first rotating disk out and send the reaction cup to a reaction cup recycling mechanism 216 (only by selecting a common recycling box) disposed on one side of the second fixed disk, where an overlapping position of the third manipulator 215 and the first fixed disk is a slow-rotation shift C6, and an overlapping position of the third manipulator 215 and the second fixed disk is a sample receiving position J1. A second sample injection needle 217 is also arranged between the second fixed disk and the detection unit 300 and is used for feeding the sample in the reaction cup on the second rotating disk into the detection unit 300; the overlapping position of the second sample injection needle 217 and the second fixed disk is a sample injection position J2, and in order to separate the residual magnetic beads in the sample, a magnetic absorber (only a common magnet is selected) is further installed on the second fixed disk of the sample injection position J2. In order to make the sample in an adjustable constant temperature environment, constant temperature incubation belts are arranged on the first fixed disk and the second fixed disk. In order to avoid sample pollution, the liquid suction needle 211, the cleaning liquid filling needle 212, the first sample injection needle 204, the double-channel reagent needle 206 and the second sample injection needle 217 are provided with needle washing mechanisms on one side. The detection unit 300 may be a liquid chromatography tandem mass spectrometry device, an immunoassay device, or a biochemical analysis device, thereby realizing various detection items.
The sample pretreatment operation of the present invention is described in three examples below.
Example 1:
the sample is pretreated by using reagents R1, R2 and R3, wherein R1 is a common reagent, R3 is a reagent containing magnetic beads, and R2 is an eluent. The above-mentioned reagents R1, R2, R3 are placed on one set of reagent placement sites on the reagent tray 205, and when the number of reagents is large, they may be placed in two or more sets. After the preparation work is finished, sample pretreatment is carried out according to the following steps:
s1: the first robot 203 takes an empty cuvette X from the empty cuvette supply unit 202 and places it in the first rotating tray of the pre-processing section 201, and at this time, the cuvette X is located at the empty cuvette placement site C1;
s2: rotating the first rotating disc to enable the reaction cup X to be rotated to a sample filling position C2, and filling one sample on the sample conveying belt 105 into the reaction cup X through the first sample injection needle 204;
s3: rotating the first rotating disk to enable the reaction cup X to be rotated to a reagent filling position C3, and filling the reagent R1 on the reagent disk 205 into the reaction cup X through the double-channel reagent needle 206;
s4: rotating the first rotating disc to enable the reaction cup X to be rotated to a mixing transfer position C4, transferring the reaction cup X to a mixing part 207 through a second mechanical arm 208 for full oscillation, transferring the reaction cup X back to the first rotating disc through the second mechanical arm 208 after the treatment is finished, and adjusting a constant-temperature incubation zone to 37 ℃ for heat preservation cultivation;
s5: repeating S3-S4, filling a magnetic bead reagent R3 into the reaction cup X, and carrying out shaking mixing and heat preservation cultivation;
s6: the first rotating disc is rotated to enable the reaction cup X to be rotated to a separation rotation shift C5, and the reaction cup X enters the magnetic washing separation cleaning part to be magnetically washed and separated and cleaned through the transfer manipulator 209, specifically: firstly, the reaction cup X reaches a magnetic suction cleaning position 213, the liquid suction needle 211 sucks the liquid in the reaction cup X after the magnetic suction, then the cleaning liquid filling needle 212 is filled with the cleaning liquid, the transfer manipulator 209 transfers the reaction cup X to the oscillation mixer 210 for uniform mixing, the reaction cup X is transferred back to the magnetic suction cleaning position 213 after the uniform mixing is finished, and the liquid suction needle 211 sucks the liquid in the reaction cup X after the magnetic suction, and the process is repeated for a plurality of times until the cleaning is finished; then moving the reaction cup X back to the first rotating disk;
s7: repeating S3-S4, filling an eluent R2 into the reaction cup X, and carrying out shaking mixing and heat preservation cultivation;
s8: rotating the first rotating disc to enable the reaction cup X to rotate to a slow-breeding rotating shift C6, and rotating the reaction cup X into the second rotating disc of the buffer incubation part 214 through the third manipulator 215, wherein the reaction cup X is positioned at the sample receiving position J1;
s9: when the sample reaches the sample-feeding state of the LC-MS/MS detection unit 300, the reaction cup X is rotated to a sample feeding position J2 integrating the magnetic attraction function through the second rotating disc, and after the sample is magnetically attracted, the second sample feeding needle 217 sucks the supernatant and transfers the supernatant to the LC-MS/MS detection unit 300;
s10: the second rotating disk is rotated to return the reaction cup X to the sample receiving position J1, and the third manipulator 215 transfers the reaction cup X after sample introduction to the reaction cup recycling mechanism 216.
Note that: in some embodiments, reagents R1, R2 may be dispensed simultaneously through dual channel reagent needle 206, i.e., S3-S5 may be combined.
Example 2:
the sample is pretreated by using reagents R1, R2 and R3, wherein R1 and R2 are common reagents, and R3 is a reagent containing a precipitant and magnetic beads. After the preparation work is finished, sample pretreatment is carried out according to the following steps:
s1: the first robot 203 takes an empty cuvette X from the empty cuvette supply unit 202 and places it in the first rotating tray of the pre-processing section 201, and at this time, the cuvette X is located at the empty cuvette placement site C1;
s2: rotating the first rotating disc to enable the reaction cup X to be rotated to a sample filling position C2, and filling one sample on the sample conveying belt 105 into the reaction cup X through the first sample injection needle 204;
s3: rotating the first rotating disk to enable the reaction cup X to be rotated to a reagent filling position C3, and filling the reagent R1 on the reagent disk 205 into the reaction cup X through the double-channel reagent needle 206;
s4: rotating the first rotating disc to enable the reaction cup X to be rotated to a mixing transfer position C4, transferring the reaction cup X to a mixing part 207 through a second mechanical arm 208 for full oscillation, transferring the reaction cup X back to the first rotating disc through the second mechanical arm 208 after the treatment is finished, and adjusting a constant-temperature incubation zone to 37 ℃ for heat preservation cultivation;
s5: repeating S3-S4, filling a reagent R2 into the reaction cup X, and carrying out shaking mixing and heat preservation cultivation;
s6: repeating S3-S4, filling a magnetic bead reagent R3 into the reaction cup X, and carrying out shaking mixing and heat preservation cultivation;
s7: rotating the first rotating disc to enable the reaction cup X to rotate to a slow-breeding rotating shift C6, and rotating the reaction cup X into the second rotating disc of the buffer incubation part 214 through the third manipulator 215, wherein the reaction cup X is positioned at the sample receiving position J1;
s8: when the sample reaches the sample-feeding state of the LC-MS/MS detection unit 300, the reaction cup X is rotated to a sample feeding position J2 integrating the magnetic attraction function through the second rotating disc, and after the sample is magnetically attracted, the second sample feeding needle 217 sucks the supernatant and transfers the supernatant to the LC-MS/MS detection unit 300;
s9: the second rotating disk is rotated to return the reaction cup X to the sample receiving position J1, and the third manipulator 215 transfers the reaction cup X after sample introduction to the reaction cup recycling mechanism 216.
Example 3:
pretreating a sample with two groups of reagents, wherein the first group comprises a reagent R1A, R2A, R A, wherein R1A, R2A is a common reagent, and R3A is a reagent comprising a precipitant and magnetic beads; the second group contains reagents R1B, R2B, R B, where R3B is a reagent comprising magnetic beads and R2B is an eluent. The two sets of reagents are placed on the two sets of reagent placement sites on the reagent storage disk 109, respectively.
When pretreatment is carried out, firstly, a sample is injected into a sample cup X, a reagent R1A, R2A, R A is added for incubation and culture, then the sample and magnetic beads are separated, a supernatant containing a target object is transferred into a new sample cup Y, a reagent R1B, R B is continuously added for incubation and culture, and the separated target object is attached to the magnetic beads and needs to be detected and analyzed after elution and separation.
The sample pretreatment is specifically carried out according to the following steps:
s1: the first robot 203 takes an empty cuvette X from the empty cuvette supply unit 202 and places it in the first rotating tray of the pre-processing section 201, and at this time, the cuvette X is located at the empty cuvette placement site C1;
s2: rotating the first rotating disc to enable the reaction cup X to be rotated to a sample filling position C2, and filling one sample on the sample conveying belt 105 into the reaction cup X through the first sample injection needle 204;
s3: rotating the first rotating disk to enable the reaction cup X to be rotated to a reagent filling position C3, and filling the reagent R1A on the reagent disk 205 into the reaction cup X through the double-channel reagent needle 206;
s4: rotating the first rotating disc to enable the reaction cup X to be rotated to a mixing transfer position C4, transferring the reaction cup X to a mixing part 207 through a second mechanical arm 208 for full oscillation, transferring the reaction cup X back to the first rotating disc through the second mechanical arm 208 after the treatment is finished, and adjusting a constant-temperature incubation zone to 37 ℃ for heat preservation cultivation;
s5: repeating S3-S4, filling the reagent R2A into the reaction cup X, and carrying out shaking mixing and heat preservation cultivation;
s6: repeating S3-S4, filling a magnetic bead reagent R3A into the reaction cup X, and carrying out shaking mixing and heat preservation cultivation;
s7: rotating the first rotating disc to enable the reaction cup X to rotate to a separation rotation shift C5, enabling the reaction cup X to enter a magnetic suction cleaning position 213 of a magnetic cleaning separation cleaning part through a transfer manipulator 209, sucking away liquid in the reaction cup X after magnetic suction through a liquid suction needle 211, and then enabling the transfer manipulator 209 to move the reaction cup X back to the first rotating disc;
s8: rotating the first rotating disk to enable the reaction cup X to rotate to a slow-breeding rotation shift C6, and conveying the reaction cup X and magnetic beads in the reaction cup X into the reaction cup recovery mechanism 216 through the third manipulator 215;
s9: rotating the first rotating disk, and taking a new empty reaction cup Y from the empty reaction cup supply unit 202 by the first manipulator 203 to be placed in the empty cup placement position C1 of the first rotating disk;
s10: rotating the first rotating disc to enable the empty reaction cup Y to rotate to a separation rotation position C5, enabling the reaction cup Y to enter a magnetic suction cleaning position 213 of a magnetic cleaning separation cleaning part through a transfer manipulator 209, driving liquid temporarily stored in a liquid suction needle 211 into the reaction cup Y, and then rotating the reaction cup Y back to the first rotating disc;
s11: repeating S3-S4, filling a reagent R1B into the reaction cup Y, and carrying out shaking mixing and heat preservation cultivation;
s12: repeating S3-S4, filling a reagent R3B into the reaction cup Y, and carrying out shaking mixing and heat preservation cultivation;
s13: the first rotating disc is rotated to enable the reaction cup Y to be rotated to a separation rotation shift C5, and the reaction cup Y enters the magnetic washing separation cleaning part to be magnetically washed and separated and cleaned through the transfer manipulator 209, specifically: firstly, the reaction cup Y reaches a magnetic suction cleaning position 213, the liquid suction needle 211 sucks the liquid in the reaction cup Y after the magnetic suction, then the cleaning liquid filling needle 212 fills the cleaning liquid, the transfer manipulator 209 transfers the reaction cup Y to the oscillation mixer 210 for uniform mixing, the reaction cup Y is transferred back to the magnetic suction cleaning position 213 after the uniform mixing is finished, and the liquid suction needle 211 sucks the liquid in the reaction cup Y after the magnetic suction for a plurality of times until the cleaning is finished; then moving the reaction cup Y back to the first rotating disk;
s14: repeating S3-S4, filling an eluent R2B into the reaction cup Y, and carrying out shaking mixing and heat preservation cultivation;
s15: rotating the first rotating disc to enable the reaction cup Y to rotate to a slow-breeding rotation shift C6, and rotating the reaction cup Y into the second rotating disc of the buffer incubation part 214 through the third manipulator 215, wherein the reaction cup Y is positioned at the sample receiving position J1;
s16: when the sample reaches the sample-feeding state of the LC-MS/MS detection unit 300, the reaction cup Y is rotated to a sample feeding position J2 integrating the magnetic attraction function through the second rotating disc, and after the sample is magnetically attracted, the second sample feeding needle 217 sucks the supernatant and transfers the supernatant to the LC-MS/MS detection unit 300;
s17: the second rotating disk is rotated to enable the reaction cup Y to return to the sample receiving position J1, and the third manipulator 215 transfers the reaction cup Y after sample injection into the reaction cup recovery mechanism 216.
It should be noted that, in the description of the present invention, terms such as "front", "rear", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element to be referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.

Claims (8)

1. A sample pretreatment method of a clinical automatic analyzer is characterized in that:
the clinical automatic analyzer comprises a sample injection unit, a sample processing unit and a detection unit which are sequentially connected, wherein the sample processing unit comprises:
the pretreatment part is provided with a first fixed disc, a first rotating disc is arranged above the first fixed disc, and the first rotating disc is used for driving the reaction cup to move along the circumferential direction;
the buffer incubation part is arranged at one side of the first fixed disc and is provided with a second fixed disc, a magnetic absorber is arranged on the second fixed disc, a second rotating disc is arranged above the second fixed disc, and the second rotating disc is used for driving the reaction cup to move along the annular direction;
the reagent storage part is arranged at one side of the first fixed disk and comprises a reagent disk provided with a plurality of groups of reagent placement positions, and each group of reagent placement positions comprises a plurality of containing holes which are sequentially arranged along the radius of the reagent disk;
the mixing part is arranged at one side of the first fixed disc and is used for placing the reaction cup and uniformly mixing the sample and the reagent in the reaction cup;
the magnetic washing separation cleaning part is arranged on one side of the first fixed disk and comprises a transfer manipulator, an oscillation mixer, a liquid suction needle and a cleaning liquid filling needle, wherein a magnetic suction cleaning position for placing a reaction cup is arranged on one side of the liquid suction needle and the cleaning liquid filling needle, and the overlapped part of the transfer manipulator and the first fixed disk is in separation rotation displacement;
the first manipulator is arranged on one side of the first fixed disc and is used for transferring the empty reaction cup to the first rotating disc, and the overlapping part of the first manipulator and the first fixed disc is an empty cup placing position;
the second manipulator is arranged between the first fixed disc and the mixing part and is used for realizing the transfer of the reaction cup between the first rotating disc and the mixing part, and the overlapping part of the second manipulator and the first fixed disc is the mixing rotation and displacement;
the third manipulator is arranged between the first fixed disc and the second fixed disc and is used for moving the reaction cup from the first rotating disc to the second rotating disc and moving the reaction cup out of the first rotating disc, the overlapped part of the third manipulator and the first fixed disc is a slow rotation shift, and the overlapped part of the third manipulator and the second fixed disc is a sample receiving position;
the first sample injection needle is arranged between the first fixed disc and the sample injection unit, and the overlapping part of the first sample injection needle and the first fixed disc is a sample filling position;
the double-channel reagent needle is arranged between the first fixed disc and the reagent storage part, and the overlapping part of the double-channel reagent needle and the first fixed disc is a reagent filling position;
the second sample injection needle is arranged between the second fixed disc and the detection unit, the overlapped part of the second sample injection needle and the second fixed disc is a sample injection position, and the magnetic absorber is arranged corresponding to the sample injection position;
for the case of adding n (n is more than or equal to 1) common reagents and containing precipitants and magnetic bead reagents into a sample, the sample pretreatment method comprises the following steps:
s1: the first manipulator takes an empty reaction cup and places the empty reaction cup in a first rotating disc of the pretreatment part, and at the moment, the reaction cup is positioned at the empty cup placing position;
s2: rotating the first rotating disc to enable the reaction cup to rotate to a sample filling position, and filling one sample in the sample filling unit into the reaction cup through the first sample injection needle;
s3: rotating the first rotating disc to enable the reaction cup to rotate to a reagent filling position, and filling a first common reagent on the reagent disc into the reaction cup through a double-channel reagent needle;
s4: rotating the first rotating disc to enable the reaction cup to be rotated to be uniformly mixed and shifted, transferring the reaction cup into the uniformly mixing part through the second manipulator to perform full oscillation, and transferring the reaction cup back to the first rotating disc through the second manipulator after the treatment is completed to perform heat preservation cultivation;
s5: sequentially carrying out S3-S4, repeating n times, and completing filling, shaking and mixing and heat preservation cultivation of other common reagents and reagents containing precipitants and magnetic beads;
s6: rotating the first rotating disc to enable the reaction cup to rotate to a slow-breeding rotation position, and rotating the reaction cup into the second rotating disc of the buffer incubation part through the third manipulator, wherein the reaction cup is positioned at the sample receiving position;
s7: rotating the second rotating disc to enable the reaction cup to rotate to a sample injection position, sucking the supernatant in the reaction cup by a second sample injection needle and transferring the supernatant to a detection unit;
s8: rotating the second rotating disc to enable the reaction cup to return to the sample receiving position, and discarding the reaction cup after sample injection by the third manipulator;
for the case of adding n (n is more than or equal to 1) common reagents, magnetic bead reagents and eluents into a sample, the sample pretreatment method comprises the following steps:
s1: the first manipulator takes an empty reaction cup and places the empty reaction cup in a first rotating disc of the pretreatment part, and at the moment, the reaction cup is positioned at the empty cup placing position;
s2: rotating the first rotating disc to enable the reaction cup to rotate to a sample filling position, and filling one sample in the sample filling unit into the reaction cup through the first sample injection needle;
s3: rotating the first rotating disc to enable the reaction cup to rotate to a reagent filling position, and filling a first common reagent on the reagent disc into the reaction cup through a double-channel reagent needle;
s4: rotating the first rotating disc to enable the reaction cup to be rotated to be uniformly mixed and shifted, transferring the reaction cup into the uniformly mixing part through the second manipulator to perform full oscillation, and transferring the reaction cup back to the first rotating disc through the second manipulator after the treatment is completed to perform heat preservation cultivation;
s5: sequentially carrying out S3-S4, repeating n times, and completing filling, vibration mixing and heat preservation cultivation of other common reagents and magnetic bead reagents;
s6: rotating the first rotating disc to enable the reaction cup to rotate to the separation rotation position, enabling the reaction cup to enter the magnetic washing separation cleaning part through the transfer manipulator to carry out magnetic washing separation cleaning, and then moving the reaction cup back to the first rotating disc;
s7: repeating S3-S4, filling eluent into the reaction cup, and carrying out shaking mixing and heat preservation cultivation;
s8: rotating the first rotating disc to enable the reaction cup to rotate to a slow-breeding rotation position, and rotating the reaction cup into the second rotating disc of the buffer incubation part through the third manipulator, wherein the reaction cup is positioned at the sample receiving position;
s9: rotating the second rotating disc to enable the reaction cup to rotate to a sample injection position, sucking the supernatant in the reaction cup by a second sample injection needle and transferring the supernatant to a detection unit;
s10: rotating the second rotating disc to enable the reaction cup to return to the sample receiving position, and discarding the reaction cup after sample injection by the third manipulator;
aiming at the condition that two groups of reagents are added into a sample, wherein the first group of reagents are m (m is more than or equal to 1) common reagents and contain precipitants, and the second group of reagents are n (n is more than or equal to 1) common reagents, magnetic bead reagents and eluents, the sample pretreatment method comprises the following steps:
s1: the first manipulator takes an empty reaction cup X and places the empty reaction cup X in a first rotating disc of the pretreatment part, and at the moment, the reaction cup X is positioned at the empty cup placing position;
s2: rotating the first rotating disc to enable the reaction cup X to be rotated to a sample filling position, and filling one sample in the sample filling unit into the reaction cup X through the first sample injection needle;
s3: rotating the first rotating disc to enable the reaction cup X to be rotated to a reagent filling position, and filling a first common reagent on the reagent disc into the reaction cup X through a double-channel reagent needle;
s4: rotating the first rotating disc to enable the reaction cup X to be rotated to be mixed and shifted, transferring the reaction cup X into the mixing part through the second manipulator to perform full oscillation, and transferring the reaction cup X back to the first rotating disc through the second manipulator after the treatment is completed to perform heat preservation cultivation;
s5: sequentially carrying out S3-S4, repeating m times to finish filling, shaking and mixing and heat preservation cultivation of other common reagents and reagents containing precipitants and magnetic beads;
s6: rotating the first rotating disc to enable the reaction cup X to rotate to a separation rotation position, enabling the reaction cup X to enter a magnetic suction cleaning position of a magnetic cleaning separation cleaning part through a transfer manipulator, sucking away liquid after magnetic suction in the reaction cup X through a liquid suction needle, and then enabling the transfer manipulator to move the reaction cup X back to the first rotating disc;
s7: rotating the first rotating disc to enable the reaction cup X to rotate to a slow-breeding rotation displacement, and sending the reaction cup X and magnetic beads in the reaction cup X out of the first rotating disc through a third manipulator;
s8: repeating S1, and placing a new empty sample cup Y on the first rotating disc at the empty cup placing position;
s9: rotating the first rotating disc to enable the empty reaction cup Y to rotate to a separation rotation position, enabling the reaction cup Y to enter a magnetic suction cleaning position of a magnetic cleaning separation cleaning part through a transfer manipulator, driving liquid temporarily stored in a liquid suction needle into the reaction cup Y, and then rotating the reaction cup Y back to the first rotating disc;
s10: sequentially carrying out S3-S4, repeating (n+1) times, and completing filling, vibration mixing and incubation of common reagents and magnetic bead reagents in the second group of reagents;
s11: rotating the first rotating disc to enable the reaction cup Y to rotate to the separation rotation displacement, enabling the reaction cup Y to enter the magnetic washing separation cleaning part through the transfer manipulator to carry out magnetic washing separation cleaning, and then moving the reaction cup Y back to the first rotating disc;
s12: repeating S3-S4, filling eluent into the reaction cup Y, and carrying out shaking mixing and heat preservation cultivation;
s13: rotating the first rotating disc to enable the reaction cup Y to rotate to a slow-breeding rotation position, and rotating the reaction cup Y into the second rotating disc of the buffer incubation part through the third manipulator, wherein the reaction cup Y is positioned at the sample receiving position;
s14: rotating the second rotating disc to enable the reaction cup Y to rotate to a sample injection position, sucking the supernatant in the reaction cup Y by a second sample injection needle, and transferring the supernatant to a detection unit;
s15: and rotating the second rotating disc to enable the reaction cup Y to return to the sample receiving position, and discarding the reaction cup Y after sample injection by the third manipulator.
2. The method for sample pretreatment of a clinical automatic analyzer according to claim 1, wherein: and constant-temperature incubation belts are arranged on the first fixed disc and the second fixed disc.
3. The method for sample pretreatment of a clinical automatic analyzer according to claim 1, wherein: an empty reaction cup supply unit is arranged on one side of the first manipulator.
4. The method for sample pretreatment of a clinical automatic analyzer according to claim 1, wherein: and one side of the third manipulator is provided with a reaction cup recycling mechanism.
5. The method for sample pretreatment of a clinical automatic analyzer according to claim 1, wherein: and needle washing mechanisms are arranged on one sides of the liquid sucking needle, the cleaning liquid filling needle, the first sample injection needle, the double-channel reagent needle and the second sample injection needle.
6. The method for sample pretreatment of a clinical automatic analyzer according to claim 1, wherein: the sample injection unit comprises
A sample conveyor belt for conveying sample tubes;
the classifying and storing disc is arranged at one side of the sample conveyor belt and is used for classifying and storing the sample tubes;
the emergency sample injection disc is arranged at one side of the classification storage disc and is used for storing sample tubes;
the sample injection pipe rack is arranged at one side of the classification storage disc and used for storing sample pipes;
the recovery pipe rack is arranged at one side of the classification storage disc and used for storing recovered sample pipes;
a first sample tube manipulator disposed between the sorting tray and the sample conveyor belt for transferring sample tubes between the sorting tray and the sample conveyor belt;
the second sample tube manipulator is arranged between the classified storage disc and the emergency sample introduction disc and is used for transferring the sample tube from the emergency sample introduction disc to the classified storage disc;
and the third sample tube manipulator is arranged between the sorting storage disc and the sample injection tube frame as well as between the sample tube and the recovery tube frame and is used for transferring the sample tubes from the sample injection tube frame to the sorting storage disc and transferring the sample tubes from the sorting storage disc to the recovery tube frame.
7. The method for sample pretreatment of a clinical automatic analyzer according to claim 6, wherein: the emergency sample introduction disc is characterized in that a first code scanner is arranged on one side of the emergency sample introduction disc, and a second code scanner is arranged near the sample introduction pipe frame and the recovery pipe frame.
8. A method of sample pretreatment for a clinical automatic analyzer according to any of claims 1 to 7, characterized in that: the detection unit is a liquid chromatography tandem mass spectrometry device, an immunoassay device or a biochemical analysis device.
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