CN217359221U - Liquid-based sample processing apparatus - Google Patents

Abstract

The utility model discloses a liquid-based sample treatment facility, include the frame and install in the functional module of frame, mobile module, retrieve module and liquid way module, functional module includes a plurality of submodule pieces that are used for sample treatment, sample treatment is accomplished in the cooperation of a plurality of submodule pieces, mobile module includes mobile guide and sliding connection in mobile guide's the material device that moves, it is used for the sample to shift between each submodule piece to move the material device, it is used for collecting the waste material that sample treatment process produced to retrieve the module, liquid way module includes actuating mechanism and reagent container, actuating mechanism is arranged in driving reagent transmission to functional module in the reagent container, with provide the required reagent of functional module in sample treatment process, mobile guide, functional module, retrieve module and liquid way module and set gradually under to from last along the direction of height of frame. The movable guide rail, the functional module, the recovery module and the liquid path module are sequentially arranged on the rack in a stacked mode, so that the space is reasonably utilized, and the occupied area is reduced.

Description

Liquid-based sample processing apparatus

Technical Field

The utility model relates to a liquid-based cell film-making technical field especially relates to a liquid-based sample treatment facility.

Background

In the prior art, the liquid-based sample preparation is usually manually carried out or a plurality of machines are assembled together and manually matched together, so that the operation of the machines assembled by a plurality of machines not only occupies a large area, but also can not realize full automation.

SUMMERY OF THE UTILITY MODEL

In view of this, the utility model provides a liquid base sample treatment facility.

The utility model provides a liquid base sample treatment facility, including the frame with install in the frame:

the functional module comprises a plurality of sub-modules for sample processing, and the plurality of sub-modules are matched to complete sample processing;

the moving module comprises a moving guide rail and a material moving device connected to the moving guide rail in a sliding mode, and the material moving device is used for transferring samples among the sub-modules;

the recovery module is used for collecting waste materials generated in the sample processing process;

the liquid path module comprises a driving mechanism and a reagent container, wherein the driving mechanism is used for driving the reagent in the reagent container to be transmitted to the functional module so as to provide the reagent required by the functional module in the sample processing process;

the movable guide rail, the functional module, the recovery module and the liquid path module are arranged along the height direction of the rack from top to bottom in sequence.

According to the technical scheme, the utility model provides a liquid-based sample treatment facility is integrated as the all-in-one with a plurality of submodule pieces that are used for sample treatment, does benefit to full automatization's operation, stacks gradually in the frame with mobile guide, functional module, recovery module and liquid way module moreover, and the rational utilization space reduces area.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

Fig. 1 is a schematic view of a first view structure of a liquid-based sample processing apparatus according to an embodiment of the present invention.

Fig. 2 is a schematic view of a second view structure of a liquid-based sample processing apparatus according to an embodiment of the present invention.

Fig. 3 is a partially enlarged schematic view at a in fig. 2.

Fig. 4 is a schematic diagram of a third view structure of a liquid-based sample processing apparatus according to an embodiment of the present invention.

Fig. 5 is a partially enlarged schematic view at B in fig. 4.

Fig. 6 is a schematic structural diagram of a rack and a material-moving module for removing a liquid-based sample processing device according to an embodiment of the present invention.

Fig. 7 is a top view of a liquid-based sample processing device according to an embodiment of the present invention, with the rack and the material-moving module removed.

Fig. 8 is an enlarged partial view of the second carriage of fig. 7 in position.

Fig. 9 is a schematic structural diagram of a first three-dimensional driving mechanism according to an embodiment of the present invention.

Fig. 10 is a schematic structural diagram of a lifting power assembly according to an embodiment of the present invention.

Fig. 11 is a schematic structural view of a first separation frame and a guide according to an embodiment of the present invention.

Fig. 12 is a schematic structural view of a first pipette tip according to an embodiment of the present invention.

Fig. 13 is a schematic structural view of a settling tube module according to an embodiment of the present invention.

In the figure, a liquid-based sample processing device 100; a frame 10; a partition plate 11; a sample feeding module 21; a sample holder 21 a; a first feeding port 211; a first feed opening 212; a centrifuge tube feed mechanism 221; a second charging port 2211; a second feed opening 2212; a centrifugal mechanism 222; a centrifuge tube blanking mechanism 223; a sedimentation dyeing module 23; a settling zone 231; a dyeing device 232; a third feed opening 233; a third feed opening 234; a tray 235; a first carrier 241; a fourth feed opening 2411; a second carrier 242; a fifth feed port 2421; a first separation frame 25; a first limiting opening 251; a limit hole 2511; side openings 2512; a guide 252; a pilot bore 2521; a second split frame 26; a second stopper 261; a first material transfer device 31; a first plug connector 311; a first connector 3111; a first three-dimensional drive mechanism 32; a first moving assembly 321; a second motor 3211; a second transmission assembly 3212; a second rail 3213; a second moving assembly 322; a first motor 3221; a first drive assembly 3222; a first rail 3223; a lifting power assembly 323; a third motor 3231; a third drive assembly 3232; third guide rail 3233; a second material transfer device 33; a second plug-in unit 331; a second plug 3311; the second three-dimensional drive mechanism 34; a third material transfer device 35; a hand grip 351; a horizontal rotation pipe-detaching motor 352; a pipe dismounting clamping tray 353; the clamping device 354; a third three-dimensional drive mechanism 36; a first recovery box 41; a second recovery box 42; a reagent container 51; an elution blending module 60; a soaking module 70; a sample container 200; centrifuging the tube 300; a settling tube module 400; a slide 401; a slide holder 402; a settling tube 403; a first pipetting tip 500; a card position portion 501; a connecting portion 502; a tip portion 503; second pipetting tip 600.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, not all, of the embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts all belong to the protection scope of the present invention.

It is also to be understood that the terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used in the specification and the appended claims, the singular forms "a", "an", and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise.

It should be further understood that the term "and/or" as used in the specification and the appended claims refers to any and all possible combinations of one or more of the associated listed items, and includes such combinations.

As shown in fig. 1-13, an embodiment of the present invention provides a liquid-based sample processing apparatus 100, which includes a frame 10, and a functional module, a moving module, a recovery module, and a liquid path module, which are mounted on the frame 10, where the functional module includes a plurality of sub-modules for sample processing, and the plurality of sub-modules cooperate to complete sample processing; the mobile module comprises a mobile guide rail and a material moving device which is connected with the mobile guide rail in a sliding mode, the material moving device is used for transferring samples among the sub-modules, the recovery module is used for collecting waste materials generated in the sample treatment process, the liquid path module comprises a driving mechanism and a reagent container 51, the driving mechanism is used for driving the reagent in the reagent container 51 to be transmitted to the functional module, and therefore the reagent required by the functional module in the sample treatment process is provided; wherein, the movable guide rail, the functional module, the recovery module and the liquid path module are sequentially arranged from top to bottom along the height direction of the frame 10.

The utility model discloses in, be integrated as the all-in-one with a plurality of submodule pieces that are used for sample treatment, do benefit to full automated operation, will remove guide rail, functional module, recovery module and liquid way module and range upon range of in proper order and set up in frame 10 moreover, the rational utilization space reduces area.

In some embodiments, as shown in fig. 6 and 7, the plurality of sub-modules includes a sample loading module 21 and a centrifuge module mounted to the rack 10, the sample loading module 21 for storing sample-loaded sample containers 200, a transfer device for transferring at least the samples from the sample containers 200 to the centrifuge tube 300; the centrifugal module comprises a centrifuge tube feeding mechanism 221 and a centrifugal mechanism 222, wherein the centrifuge tube feeding mechanism 221 is used for feeding centrifuge tubes 300, and the centrifugal mechanism 222 is a centrifuge used for centrifuging samples loaded in the centrifuge tubes 300; as shown in fig. 1 and fig. 2, the sample loading module 21 is provided with a first loading port 211 for loading the sample container 200, the centrifuge tube loading mechanism 221 is provided with a second loading port 2211 for loading the centrifuge tube 300, and the first loading port 211 and the second loading port 2211 are located on the same side of the rack 10.

Illustratively, the sample feeding module 21 includes a sample rack 21a for storing the sample containers 200, a sealing cover capable of being punctured is disposed above the sample containers 200, so as to ensure that the samples collected in the sample containers 200 are not polluted by the external environment, when the sample liquid in the sample containers 200 needs to be sucked, the sample containers 200 containing the collected samples can be placed on the sample rack 21a by puncturing the sealing cover above, and after the sample rack 21a is full of the sample containers 200, the sample rack 21a is completely fed from the first feeding port 211, so that the sample containers 200 are placed on the rack 10; centrifuge tube feed mechanism 221 is for depositing the centrifuge tube rack of unloaded centrifuge tube 300, and after centrifuge tube rack filled centrifuge tube 300, accomplished the material loading through second material loading mouth 2211 for centrifuge tube 300 places on frame 10, locates the same side of frame 10 with first material loading mouth 211 and second material loading mouth 2211, can make things convenient for the manual work to carry out the material loading of sample container 200 and centrifuge tube 300 with same one side.

In some embodiments, as shown in fig. 1, 2, 6, and 7, the sample loading module 21 further has a first unloading opening 212 for unloading the sample container 200, the centrifuge module further includes a centrifuge tube unloading mechanism 223 for unloading the centrifuge tube 300, the centrifuge tube unloading mechanism 223 has a second unloading opening 2212 for unloading the centrifuge tube 300, and the first unloading opening 212, the second unloading opening 2212, and the first loading opening 211 are located on the same side of the rack 10.

Illustratively, the sample feeding module 21 includes two sample holders 21a arranged side by side along a horizontal direction, wherein one sample holder 21a is used for feeding the sample container 200 through the first feeding port 211, the other sample holder 21a is used for storing the sample container 200 with at least a part of the sample liquid removed, after the sample container 200 with at least a part of the sample liquid removed is filled, the sample holder 21a is discharged from the first discharging port 212, the sample containers 200 with the rest sample liquid can be stored for later review, and the sample containers 200 with the sample liquid completely removed are discharged and recycled; centrifuging tube unloading mechanism 223 sets up side by side along the horizontal direction with centrifuging tube feed mechanism 221, centrifuging tube unloading mechanism 223 is used for depositing and is equipped with sample liquid and waits to carry out centrifugal processing's centrifuging tube 300 or loads the centrifuging tube 300 of the processing fluid that has accomplished centrifugal processing, the processing fluid that accomplishes centrifugal processing is taken away at least after partially, centrifuging tube 300 can carry out the unloading from second feed opening 2212, centrifuging tube 300 of remaining processing fluid can deposit well so that follow-up reinspection uses in addition, with first feed opening 212, second feed opening 2212, the homonymy of frame 10 is all located to first material loading 211 and second material loading 2211, be convenient for the manual work carry out the unloading of sample container 200 and centrifuging tube 300 with one side.

In some embodiments, as shown in fig. 1-3, 6 and 7, the plurality of sub-modules further includes a sedimentation staining module 23 mounted on the rack 10, the sedimentation staining module 23 is configured to deposit the sample centrifuged by the centrifugation module onto a slide 401 of the sedimentation tube module 400 and stain the sample deposited on the slide 401, and the material transferring device is further configured to transfer the sample in the centrifugation tube 300 to the sedimentation tube module 400; the sedimentation dyeing module 23 is provided with a third feeding port 233 and a third discharging port 234, wherein the third feeding port 233 is used for supporting the feeding of the material tray with the sedimentation tube module 400, and the third discharging port 234 is used for discharging the material tray; the third feed opening 233, the third feed opening 234 and the first feed opening 211 are all located on the same side of the housing 10.

In a specific application, the sedimentation tube module 400 is a consumable material required to be used in a sheet making process, as shown in fig. 13, the sedimentation tube module 400 includes a slide for bearing a sample, a slide holder 402 for mounting the slide, and a sedimentation tube 403 for settling the sample on the slide, the slide is pre-loaded in the slide holder 402, and the sedimentation tube 403 is clamped and fixed to form the sedimentation tube module 400. The material tray is filled with the sedimentation tube module 400 which is loaded with glass slides in advance and then is loaded through the third loading port 233, the empty material tray which is taken away from the sedimentation tube module 400 is unloaded from the third unloading port 234, after the sedimentation tube module 400 is received by the liquid-based sample processing equipment 100, the liquid-based sample processing equipment 100 can independently complete a series of steps for producing the slide, finally the prepared sample slide is output from the liquid-based sample processing equipment 100, and the first loading port 211, the first unloading port 212, the second loading port 2211, the second unloading port 2212, the third loading port 233 and the third unloading port 234 are all arranged on the same side of the rack 10, so that the loading of the material tray of the sample container 200, the centrifuge tube 300 and the loading sedimentation tube module 400 and the unloading of the sample container 200, the centrifuge tube 300 and the empty material tray can be manually carried out on the same side.

Illustratively, as shown in fig. 7, the sedimentation staining module 23 includes a sedimentation region 231 for settling the sample, and further includes a staining device 232 for staining the sample settled on the slide 401 in the sedimentation region 231, after the sedimentation tube module 400 is loaded from the third loading port 233, the sample may be transferred into the sedimentation region 231 by a transfer device such as a conveyor belt and/or a robot, the transfer device transfers the sample after the centrifugal treatment and the buffer solution blending are completed to the sedimentation tube module 400 during the transfer process, the sedimentation tube module 400 enters the sedimentation region 231 after the sample is loaded, the useful cells in the sample settle on the slide 401 from the sedimentation tube 403, and then the staining device 232 stains the sample settled on the slide 401.

In some embodiments, as shown in fig. 6 and 7, the sub-module further comprises a pipetting tip loading module comprising a first carrier 241 and a second carrier 242, the first carrier 241 for storing the first pipetting tip 500 and the second carrier 242 for storing the second pipetting tip 600; the material transferring device comprises a first material transferring device 31 and a second material transferring device 33, wherein the first material transferring device 31 is used for plugging a first liquid transferring suction head 500 and transferring a sample from the sample container 200 to the centrifuge tube 300 through the first liquid transferring suction head 500; the second material transferring device 33 is used for inserting the second pipetting tip 600 and transferring the sample in the centrifuge tube 300 to the sedimentation tube module 400 through the second pipetting tip 600.

Exemplarily, first liquid-transfering suction head 500 can adopt the specification that the volume is 10ml, and is capacious, conveniently transfer the sample stoste in sample container 200 to centrifuging tube 300, the processing solution after centrifugal treatment adds buffer solution mixing again and transfers to sedimentation tube module 400 through second liquid-transfering suction head 600, the volume that needs to shift this moment is far less than first liquid-transfering suction head 500's the volume that shifts, second liquid-transfering suction head 600 can adopt the specification that the volume is 1ml, the precision problem of the volume that shifts is guaranteed to the benefit accuracy when shifting the processing solution through 1ml second liquid-transfering suction head 600, thereby improve the precision when follow-up film-making, and can reduce the cost when satisfying the liquid-transfering demand.

In some embodiments, as shown in fig. 1 and 2, the first carrier 241 is provided with a fourth loading port 2411 for loading the first pipetting tip 500, and the second carrier 242 is provided with a fifth loading port 2421 for loading the second pipetting tip 600; the fourth material loading port 2411, the fifth material loading port 2421 and the first material loading port 211 are all located on the same side of the frame 10. In the present embodiment, in order to improve the sample transfer efficiency, the first bearing frame 241 and the second bearing frame 242 are disposed near the device to be transferred, so as to save the sample transfer time and improve the working efficiency of the liquid-based sample processing apparatus 100, the first loading port 211, the first unloading port 212, the fourth loading port 2411, the second loading port 2211, the fifth loading port 2421, the second unloading port 2212, the third loading port 233 and the third unloading port 234 are sequentially arranged on the same side of the rack 10, so that the loading of the sample container 200, the centrifuge tube 300, the first pipetting tip 500, the second pipetting tip 600 and the tray of the loading sedimentation tube module 400 and the loading of the sample container 200, the centrifuge tube 300 and the empty tray can be performed manually on the same side. It should be noted that the first and second pipetting tips 500 and 600 are disposable consumables, and when the first pipetting tip 500 on the first bearing rack 241 and the second pipetting tip 600 on the second bearing rack 242 are used up, the first pipetting tip 500 and the second pipetting tip 600 can be loaded from the fourth loading port 2411 and the fifth loading port 2421 on the same side, respectively, which is convenient for manual operation.

In some embodiments, as shown in fig. 7, 8 and 11, the liquid-based sample processing apparatus 100 further comprises a first separation rack 25 and a second separation rack 26 mounted on the rack 10, the first separation rack 25 is provided with a first limiting opening 251 for separating the first pipetting tip 500 from the first material-moving device 31; the second separation rack 26 is provided with a second limit port 261 for separating the second pipetting tip 600 from the second pipetting device 33, so that the first pipetting tip 500 and the second pipetting tip 600 can be conveniently detached and recovered.

In some embodiments, as shown in fig. 1-5, 9 and 10, the moving guide comprises a first three-dimensional driving mechanism 32 and a second three-dimensional driving mechanism 34, the first material moving device 31 comprises a first connector 311 connected with the first three-dimensional driving mechanism 32, the first connector 311 is used for connecting the first pipetting tip 500, and the first three-dimensional driving mechanism 32 is mounted on the top layer of the rack 10 and is used for driving the first connector 311 to move in three-dimensional directions; the second material moving device 33 comprises a second connector 331 connected with a second three-dimensional driving mechanism 34, the second connector 331 is used for connecting a second liquid-transferring sucker 600, and the second three-dimensional driving mechanism 34 is arranged on the top layer of the frame 10 and is used for driving the second connector 331 to move in three-dimensional directions.

Illustratively, the first three-dimensional driving mechanism 32 and the second three-dimensional driving mechanism 34 may adopt the same type of driving manner, and specifically include a first moving assembly 321, a second moving assembly 322, and a lifting power assembly 323, where the first moving assembly 321 is mounted on the second moving assembly 322, and the lifting power assembly 323 is mounted on the first moving assembly 321.

As shown in fig. 9 and 10, the first three-dimensional drive mechanism 32 will be described as an example. The first connector 311 is attached to the elevating power unit 323 of the first three-dimensional driving mechanism 32. The first moving assembly 321 is used for driving the lifting power assembly 323 to drive the first plug connector 311 to reciprocate along a first horizontal direction, the second moving assembly 322 is used for driving the first moving assembly 321 to drive the lifting power assembly 323 and the first plug connector 311 to reciprocate along a second horizontal direction, and the first horizontal direction is perpendicular to the second horizontal direction. It should be noted that the lifting power assembly 323 can drive the first plug connector 311 to reciprocate along the vertical direction, that is, can drive the first plug connector 311 to lift. And the first horizontal direction, the second horizontal direction and the vertical direction are mutually vertical. That is, the second moving assembly 322, the first moving assembly 321, and the elevating power assembly 323 constitute the first three-dimensional driving mechanism 32 movable in three axes.

As shown in fig. 9, as an embodiment, the second moving assembly 322 includes a first motor 3221, a first transmission assembly 3222 and a first guide track 3223, the first motor 3221 is in transmission connection with the first transmission assembly 3222, the first moving assembly 321 is slidably installed on the first guide track 3223, and the first moving assembly 321 is fixedly connected to the first transmission assembly 3222. The first motor 3221 rotates to drive the first moving assembly 321, the lifting power assembly 323 mounted on the first moving assembly 321, and the first plug connector 311 mounted on the lifting power assembly 323 to slide along the first guide rail 3223 through the first transmission assembly 3222. It should be noted that, in this embodiment, two sets of second moving assemblies 322 are provided, and the two sets of second moving assemblies 322 are respectively disposed on two opposite sides, and since the second moving assemblies 322 need to drive the first moving assembly 321, the lifting power assembly 323, and the first plug connector 311, considering that more objects need to be driven and the weight is large, the two sets of second moving assemblies 322 are provided to ensure the stability in the moving process.

As shown in fig. 9, as an embodiment, the first moving assembly 321 includes a second motor 3211, a second transmission assembly 3212, and a second guide rail 3213, the second motor 3211 is in transmission connection with the second transmission assembly 3212, the lifting power assembly 323 is slidably mounted on the second guide rail 3213, and the lifting power assembly 323 is fixedly connected to the second transmission assembly 3212. The second motor 3211 rotates to drive the lifting power assembly 323 and the first connector 311 mounted on the lifting power assembly 323 to slide along the second guide rail 3213 through the second transmission assembly 3212. It should be noted that, in the present embodiment, only one set of first moving component 321 is provided, and since the first moving component 321 only needs to drive the lifting power component 323 and the first plug connector 311, and the weight of the object to be driven is not large, only one set of first moving component 321 is provided to reduce the space occupied by the device.

As shown in fig. 9 and 10, as an embodiment, the lifting power assembly 323 includes a third motor 3231, a third transmission assembly 3232, and a third guide rail 3233, the third motor 3231 is in transmission connection with the third transmission assembly 3232, and the first plug connector 311 is slidably mounted on the third guide rail 3233. The third motor 3231 rotates to drive the first connector 311 to slide along the third guiding rail 3233 via the third transmission assembly 3232. It should be noted that, in this embodiment, two sets of lifting power assemblies 323 are provided, the number of the lifting power assemblies 323 should be the same as the number of the first limiting openings 251, each set of lifting power assemblies 323 corresponds to one first connector 311, and the two sets of lifting power assemblies 323 can simultaneously connect two first liquid-transfer suction heads 500, so as to improve the working efficiency of the apparatus.

As shown in fig. 9 and 10, the first transmission assembly 3222, the second transmission assembly 3212 and the third transmission assembly 3232 are any one of a belt transmission mechanism, a chain transmission mechanism, a rack and pinion transmission mechanism or a screw transmission mechanism, for example. It should be noted that what the first drive assembly 3222 and the second drive assembly 3212 of this embodiment adopted is the belt conveying mechanism, because the utility model discloses a second removes the scope that subassembly 322 and first removal subassembly 321 need remove great, and belt conveying mechanism can satisfy this demand, and takes conveying mechanism's transmission steady, simple structure, with low costs, use to maintain convenient, can effectively reduce the cost of equipment and the maintenance of the later stage of being convenient for. Of course, in particular applications, the first drive assembly 3222 and the second drive assembly 3212 are not limited to use with belt-driven mechanisms, for example, as an alternative, a chain-driven mechanism, or a rack and pinion drive, or a screw drive, etc., may also be used. What the third transmission assembly 3232 of this embodiment adopted is screw drive mechanism, because the utility model discloses in, the required scope that removes of lift power assembly 323 is less and require the precision higher, and screw drive has the precision height, longe-lived. The advantage of smooth operation, so the third driving assembly 3232 in this embodiment uses a screw driving mechanism as the optimal solution. Of course, in particular applications, the third drive assembly 3232 is not limited to use with a screw drive mechanism, for example, a belt drive mechanism, or a chain drive mechanism, or a rack and pinion drive mechanism, etc. may be used instead. The second three-dimensional driving mechanism 34 can drive the second connector 331 to move in the same manner as the first three-dimensional driving mechanism 32, and will not be described in detail herein.

In specific application, the second three-dimensional driving mechanism 34 can also be used for grabbing the centrifuge tube 300 into the centrifuge, and the clamping jaws for grabbing the centrifuge tube 300 and the second plug-in connector 331 for plugging the second liquid-transfer pipette tip 600 are arranged at intervals and do not interfere with each other.

In some embodiments, the first connector 311 is provided with at least two first connectors 3111 for connecting the first pipetting tips 500, such that the first connectors 3111 can be connected with at least two first pipetting tips 500 at the same time and can transfer the sample liquids in at least two sample containers 200 into at least two centrifuge tubes 300; and/or, the second plug-in piece 331 is provided with at least two second plug-in connectors 3311 for plugging the second liquid-transfering tips 600, so that the second plug-in connectors 3311 plug in at least two second liquid-transfering tips 600 at the same time and transfer the treatment liquid in at least two centrifuge tubes 300 to at least two sedimentation tube modules 400 respectively. In this embodiment, as shown in fig. 10, the first connector 311 can be simultaneously connected with two first pipetting tips 500 to simultaneously transfer part of the sample liquid in two sample containers 200 into two centrifuge tubes 300, and the second connector 331 can be simultaneously connected with two second pipetting tips 600 to simultaneously transfer part of the sample liquid in two centrifuge tubes 300 into two sedimentation tube modules 400, so as to improve the sample transfer efficiency.

Illustratively, the top of the first liquid-transferring pipette tip 500 is provided with a jack adapted to the first connection-peg 3111, the top of the second liquid-transferring pipette tip 600 is provided with a jack adapted to the second connection-peg 3311, the first liquid-transferring pipette tip 500 and the second liquid-transferring pipette tip 600 are both made of plastic material and have certain elasticity, the ends of the first connection-peg 3111 and the second connection-peg 3311 are cone-shaped heads, and the outer diameter of the first connector 3111 is slightly larger than the diameter of the top jack of the first liquid-transferring pipette tip 500, the outer diameter of the second connector 3311 is slightly larger than the diameter of the top jack of the second liquid-transferring pipette tip 600, thus, not only the first connector 3111 and the second connector 3311 can be ensured to be inserted into the corresponding sockets under the elastic action, but also the first connector 3111 and the first liquid-transferring pipette tip 500 and the second connector 3311 and the second liquid-transferring pipette tip 600 can be ensured to be detachably connected, so that the first and second pipetting tips 500, 600 can be inserted and detached.

Illustratively, as shown in fig. 12, the first and second pipetting tips 500 and 600 provided by the present invention have different sizes, but have the same or similar shapes, and each include a positioning portion 501, a connecting portion 502 and a tip portion 503, and the first pipetting tip 500 in the figure is taken as an example for description, the connecting portion 502 is connected to the positioning portion 501 and the tip portion 503, and the tip portion 503 is used for sucking the sample liquid, the connecting portion 502 is used for storing the sample liquid, the minimum diameter of the positioning portion 501 of the first pipetting tip 500 should be larger than the width of the opening of the first limiting opening 251, so that the first connector 311 can pass through the first limiting opening 251 and the positioning portion 501 of the first pipetting tip 500 cannot pass through the first limiting opening 251, thereby separating the first pipetting tip 500 from the first pipetting device 31.

Similarly, the minimum diameter of the blocking part 501 of the second pipetting tip 600 should be larger than the width of the opening of the second limiting opening 261, so that the second plug-in part 331 can pass through the second limiting opening 261 and the blocking part 501 of the second pipetting tip 600 cannot pass through the second limiting opening 261, thereby separating the second pipetting tip 600 from the second pipetting device 33.

Optionally, the first separation frame 25 is provided with more than two first limiting openings 251, and the second separation frame 26 is provided with more than two second limiting openings 261. In this embodiment, as shown in fig. 11, the first separation frame 25 is provided with two first limiting ports 251, the second separation frame 26 is provided with two second limiting ports 261, a certain distance is provided between the two first limiting ports 251 and between the two second limiting ports 261, the two first limiting ports 251 can be arranged to disassemble and recover the two first pipetting tips 500 at the same time, the two second limiting ports 261 can be arranged to disassemble and recover the two second pipetting tips 600 at the same time, and the working efficiency of the device is improved. Of course, in a particular application, the number of the first position-limiting apertures 251 and the second position-limiting apertures 261 is not limited to two, but may be, for example, three or four as an alternative.

As an embodiment, as shown in fig. 11, the first position-limiting opening 251 penetrates through the first separation rack 25, the second position-limiting opening 261 penetrates through the second separation rack 26, both the first position-limiting opening 251 and the second position-limiting opening 261 include a side opening 2512 and a position-limiting hole 2511, the side opening 2512 is communicated with the position-limiting hole 2511, the outer diameter of the first connector 3111 is smaller than the width of the side opening 2512 of the first position-limiting opening 251 and smaller than the diameter of the position-limiting hole 2511 of the first position-limiting opening 251, so that the first connector 3111 can pass through the first position-limiting opening 251, and the first pipetting tip 500 cannot pass through the first position-limiting opening 251. Similarly, the outer diameter of the second plug 3311 is smaller than the width of the side opening 2512 of the second limiting port 261 and smaller than the aperture of the limiting hole 2511 of the second limiting port 261, so that the second plug 3311 can pass through the second limiting port 261, and the second pipetting tip 600 cannot pass through the second limiting port 261, thereby facilitating the separation and recovery of the first pipetting tip 500 and the second pipetting tip 600.

As an embodiment, the side openings 2512 of the two first position-limiting ports 251 face the same side of the first separating rack 25, the side openings 2512 of the two second position-limiting ports 261 face the same side of the second separating rack 26, and the two first position-limiting ports 251 and the two second position-limiting ports 261 face the same side, so as to facilitate the operation, so that the first connector 3111 drives the first liquid-transferring tip 500 to enter from the same side, and the second connector 3311 drives the second liquid-transferring tip 600 to enter from the same side, thereby improving the working efficiency of the device.

Illustratively, the number of the lifting power components 323 of the first three-dimensional driving mechanism 32 and the number of the first plugging heads 3111 are the same as the number of the first limiting ports 251, the number of the lifting power components 323 of the second three-dimensional driving mechanism 34 and the number of the second plugging heads 3311 are the same as the number of the second limiting ports 261, in this embodiment, two lifting power components 323 of the first three-dimensional driving mechanism 32 corresponding to the two first limiting ports 251 are further provided, and the horizontal distance between the two first limiting ports 251 is equal to the horizontal distance between the two corresponding lifting power components 323, so that the two first pipetting tips 500 can be driven to perform the recovery operation, and the efficiency is improved. Similarly, the embodiment is provided with two second limiting openings 261 and two lifting power assemblies 323 of the second three-dimensional driving mechanism 34 corresponding to the two second limiting openings 261, so that the two second pipetting tips 600 can be driven to perform the recovery operation, and the efficiency is improved.

In some embodiments, as shown in fig. 1, 2 and 6, the rack 10 comprises a partition 11 for separating the functional module and the recovery module, the functional module is mounted on the partition 11, the recovery module is located below the partition 11, the partition 11 is provided with a first recovery window and a second recovery window, the first recovery window is located below the first limiting opening 251, the second recovery window is located below the second limiting opening 261, and the recovery module comprises a first recovery box 41 located below the first recovery window and the second recovery window, so that the separated first pipetting tip 500 and the separated second pipetting tip 600 fall into the first recovery box 41 from the first recovery window and the second recovery window, respectively, for recovery. In this embodiment, the first recovery box 41 is similar to a drawer in principle, and when the first pipetting tip 500 and the second pipetting tip 600 in the first recovery box 41 are full, the first recovery box 41 can be directly drawn out for cleaning and then returned for further use.

Exemplarily, as shown in fig. 11, the first separation frame 25 and the second separation frame 26 are further provided with a guide 252, the guide 252 of the first separation frame 25 is disposed through the first recovery window and between the first recovery box 41 and the first limit opening 251, and the guide 252 of the second separation frame 26 is disposed through the second recovery window and between the first recovery box 41 and the second limit opening 261. The guide 252 used in this embodiment is a housing having a square column shape, the guide 252 has guide holes 2521 penetrating both ends in the longitudinal direction thereof, the guide holes 2521 are square holes, and the guide holes 2521 of the first separation rack 25 are located right below the first stopper 251, so that the first pipette tip 500 can smoothly drop into the first recovery box 41 through the guide holes 2521 after dropping off. The guide hole 2521 of the second separation rack 26 is positioned directly below the second stopper 261, so that the second pipette tip 600 can smoothly drop into the first recovery box 41 through the guide hole 2521 after dropping. Of course, in a specific application, the guiding element 252 is not limited to a housing with a square column, for example, as an alternative, it may also be a housing with a cylindrical shape, and the corresponding guiding hole 2521 is a circular hole.

In some embodiments, slide 401 is removably attached to slide holder 402 by a sedimentation tube 403; the material moving device further comprises a third material moving device 35, wherein the third material moving device 35 is used for disassembling the sedimentation tube module 400 after sedimentation dyeing is completed, so that the glass slide 401 is separated from the glass slide fixing frame 402 and the sedimentation tube 403.

In some embodiments, as shown in fig. 1-2, the rack 10 includes a partition 11 for separating the functional module and the recovery module, the functional module is mounted on the partition 11, the recovery module is located below the partition 11, the partition 11 is provided with a third recovery window located below the third material moving device 35, and the recovery module includes a second recovery box 42 located below the third recovery window, so that the detached slide holder 402 and the sedimentation tube 403 fall into the second recovery box 42 from the third recovery window for recovery. In this embodiment, the second recovery box 42 may be designed as a drawer-type structure, and when the slide holder 402 and the sedimentation tube 403 in the second recovery box 42 are full, they can be drawn out and cleaned and then placed back for further use.

For example, as shown in fig. 1 to fig. 3, the moving guide rail further includes a third three-dimensional driving mechanism 36 installed on the top layer of the rack 10, and the third three-dimensional driving mechanism 36 may drive the third material moving device 35 to move in the same manner as the first three-dimensional driving mechanism 32, that is, the guide rail and the transmission mechanisms such as the motor are installed in the x, y, and z directions, so as to implement the spatial movement of the carrier, which is not described herein again.

In specific application, the sedimentation tube 403 is connected to the slide holder 402 by a rotary buckle and locks the slide 401, so that the sedimentation tube 403 can be detached by only rotating the sedimentation tube 403 during detachment.

Illustratively, as shown in fig. 2 and 3, the third material transferring device 35 includes a hand grip 351 and a horizontal rotation tube detaching motor 352, the hand grip 351 is used for gripping the sedimentation tube module 400 on a tube detaching holding tray 353 mounted on the frame 10, the tube detaching holding tray 353 is provided with a clamping groove for limiting the rotation of the slide holder 402, then the hand grip 351 is controlled by the horizontal rotation tube detaching motor 352 to rotate to grip the sedimentation tube 403 to a certain angle, so that the sedimentation tube 403 is detached from the slide 401 and the slide holder 402, then the detached sedimentation tube 403 is transferred to the second recovery box 42 to finish discarding the sedimentation tube 403, after the sedimentation tube 403 is detached, the slide holder 402 with the slide 401 is rotated by 90 ° to stand vertically with the socket of the slide holder 402 facing upwards by a clamping device 354 capable of driving the slide holder 402 to rotate, then the slide 401 on the slide holder 402 is gripped by the hand grip 351 to be extracted from the socket, to separate the slide 401 from the slide holder 402, and the gripping device 354 is rotated 180 ° in reverse after the slide 401 is disengaged to drop the slide holder 402 into the second recovery cassette 42.

In specific practical operation, firstly, the sample liquid subjected to centrifugal treatment is sucked by the second liquid-transferring suction head 600 and dripped onto the glass slide 401 through the upper part of the sedimentation tube 403, after a period of time, the dyeing device 232 drives the dyeing needle to reach the upper part of the sedimentation tube 403 to dye the sample attached to the glass slide 401, after the dyeing is finished and waited for a period of time, the third material-transferring device 35 disassembles the sedimentation tube module 400, after the disassembles, the glass slide 401 is soaked, and after the soaking, the sheet-making process of the liquid-based sample treatment equipment 100 is completed.

In this embodiment, the first three-dimensional driving device, the second three-dimensional driving device, and the third three-dimensional driving device are respectively located in different areas of the top layer of the rack 10, and the spatial layout is reasonable, so that interference between the first three-dimensional driving device and the second three-dimensional driving device is avoided, and the working efficiency is high.

In some embodiments, as shown in fig. 6 and 7, the liquid-based sample processing apparatus 100 further includes a soaking module 70, where the soaking module 70 includes an absolute ethanol soaking cylinder and a xylene soaking cylinder, so as to perform absolute ethanol soaking on the disassembled slide glass 401 to clean the sample for excess dyeing solution and dehydration, and then perform xylene soaking on the slide glass 401 to make the sample transparent, so as to facilitate later observation and preservation, and perform xylene soaking to ensure that the slide glass 401 is isolated from air, and complete the slide making process of the liquid-based sample processing apparatus 100 after soaking.

In a specific application, the plurality of reagent containers 51 of the liquid path module are loaded with all reagents required in the slide preparation process, such as buffer solution, separation solution, staining agent required for staining treatment, absolute ethanol required for dehydration treatment, xylene for making the sample transparent, and the like. The driving mechanism is a pump body which can drive the reagent in the reagent container 51 to be transported to a required position through a pipeline. In this embodiment, the liquid path module is disposed at the bottom layer of the rack 10, so as to facilitate the arrangement and placement of the driving mechanism and the reagent container 51, and to reasonably utilize the space.

For example, the frame 10 may have a plate-like structure, and it is understood that the frame 10 has a housing structure or a frame structure including a support member such as a support plate or a support rod. The frame 10 may have supporting legs or wheels mounted thereon to facilitate mounting and movement of the liquid-based sample processing device 100. In the description of some embodiments, the specific components of each functional unit, if not specifically described, may be directly or indirectly fixed to the frame 10.

In some embodiments, as shown in fig. 6 and 7, the liquid-based sample processing apparatus 100 further includes an elution and mixing module 60, and the elution and mixing module 60 is configured to elute a sample from a swab or a sampling brush disposed in the sample container 200 into a preservation solution in the sample container 200, and mix the sample with the preservation solution to form a sample solution.

The embodiment of the utility model provides an among the liquid-based sample processing equipment 100, the user only need place the sample in sample material loading module 21 to and supplement some consumptive materials, then other middle steps of whole film-making, like steps such as sample elution mixing, centrifugal treatment, settlement dyeing, soaking treatment, all can be accomplished by liquid-based sample processing equipment 100 is automatic, in order to obtain the sample slide that prepares.

Above-mentioned whole film-making process only needs the user to provide raw materials, consumptive material and tool in the periphery, and all middle-process all need not manual operation's intervention, therefore degree of automation is high, can promote film-making efficiency by a wide margin. Meanwhile, because the intervention of manual operation is reduced, the devices such as slides do not need to be exposed in the external environment because the times of manual transfer and other operations are reduced, the risk that the devices are polluted in the external environment is also reduced, and the safety and result accuracy of slide preparation are improved.

The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily think of various equivalent modifications or replacements within the technical scope of the present invention, and these modifications or replacements should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims (10)

1. A liquid-based sample processing apparatus comprising a frame and, mounted to the frame:

the functional module comprises a plurality of sub-modules for sample processing, and the plurality of sub-modules are matched to complete sample processing;

the moving module comprises a moving guide rail and a material moving device connected to the moving guide rail in a sliding mode, and the material moving device is used for transferring samples among the sub-modules;

the recovery module is used for collecting waste materials generated in the sample processing process;

the liquid path module comprises a driving mechanism and a reagent container, wherein the driving mechanism is used for driving the reagent in the reagent container to be transmitted to the functional module so as to provide the reagent required by the functional module in the sample processing process;

the movable guide rail, the functional module, the recovery module and the liquid path module are arranged along the height direction of the rack from top to bottom in sequence.

2. The liquid-based sample processing apparatus of claim 1, wherein the plurality of sub-modules comprises, mounted to the rack:

the sample loading module is used for storing a sample container loaded with the sample, and the material moving device is at least used for transferring the sample from the sample container to a centrifugal tube;

the centrifugal module comprises a centrifugal tube feeding mechanism and a centrifugal mechanism, the centrifugal tube feeding mechanism is used for feeding the centrifugal tube, and the centrifugal mechanism is used for carrying out centrifugal treatment on a sample loaded in the centrifugal tube;

wherein, sample material loading module is equipped with the first material loading mouth that is used for the sample container material loading, centrifuging tube feed mechanism is equipped with the second material loading mouth that is used for the centrifuging tube material loading, first material loading mouth with the second material loading mouth is located the same side of frame.

3. The liquid-based sample processing device according to claim 2, wherein the sample loading module is further provided with a first feed opening for unloading the sample container, the centrifuge module further comprises a centrifuge tube unloading mechanism for unloading the centrifuge tube, the centrifuge tube unloading mechanism is provided with a second feed opening for unloading the centrifuge tube, and the first feed opening, the second feed opening and the first feed opening are located on the same side of the rack.

4. The liquid-based sample processing apparatus of claim 2, wherein the plurality of sub-modules further comprises, mounted to the rack:

the sedimentation dyeing module is used for settling the sample subjected to centrifugal treatment by the centrifugal module on a glass slide of the sedimentation tube module and dyeing the sample settled on the glass slide, and the material moving device is also used for transferring the sample in the centrifugal tube to the sedimentation tube module;

the sedimentation dyeing module is provided with a third feeding hole and a third discharging hole, and the third feeding hole is used for supporting the feeding of a material tray with a sedimentation tube module and the third discharging hole is used for discharging the material tray; the third feed opening, the third feed opening and the first feed opening are all located on the same side of the rack.

5. The liquid-based sample processing apparatus of claim 4, wherein the plurality of sub-modules further comprises a pipette tip loading module comprising a first carrier for storing a first pipette tip and a second carrier for storing a second pipette tip;

the material moving device comprises:

the first material transferring device is used for inserting the first liquid transferring suction head and transferring the sample from the sample container to a centrifuge tube through the first liquid transferring suction head;

and the second material moving device is used for inserting the second liquid-transferring sucker and transferring the sample in the centrifugal pipe to the sedimentation pipe module through the second liquid-transferring sucker.

6. The liquid-based sample processing apparatus of claim 5, wherein the first carrier is provided with a fourth loading port for loading of the first pipetting tip, and the second carrier is provided with a fifth loading port for loading of the second pipetting tip;

the fourth feeding port, the fifth feeding port and the first feeding port are all located on the same side of the rack.

7. The liquid-based sample processing device of claim 5, further comprising:

the first separating frame is arranged on the rack and provided with a first limiting opening for separating the first liquid-transferring sucker from the first material-transferring device;

and the second separating frame is arranged on the rack and is provided with a second limiting port for separating the second liquid-transferring suction head from the second material-transferring device.

8. The liquid-based sample processing apparatus of claim 7, wherein the rack comprises a partition for separating the functional module and the recovery module, the functional module is mounted on the partition, the recovery module is located below the partition, the partition is provided with a first recovery window and a second recovery window, the first recovery window is located below the first limit port, the second recovery window is located below the second limit port, and the recovery module comprises a first recovery box located below the first recovery window and the second recovery window, so that the separated first pipetting tip and the separated second pipetting tip fall from the first recovery window and the second recovery window into the first recovery box for recovery.

9. The liquid-based sample processing apparatus of claim 4, wherein the sedimentation tube module comprises a slide mount, a sedimentation tube, and the slide, the slide being removably attached to the slide mount via the sedimentation tube;

the material moving device further comprises a third material moving device, and the third material moving device is used for disassembling the sedimentation tube module after sedimentation dyeing is completed, so that the glass slide is separated from the glass slide fixing frame and the sedimentation tube.

10. The liquid-based specimen processing apparatus of claim 9, wherein the rack includes a partition for separating the functional module and the recovery module, the functional module is mounted on the partition, the recovery module is located below the partition, the partition is provided with a third recovery window located below the third material moving device, and the recovery module includes a second recovery box located below the third recovery window, so that the detached slide holder and the sedimentation tube fall from the third recovery window into the second recovery box for recovery.

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