CN114437935B - Method for increasing oil content of microalgae produced by culturing smoke - Google Patents
Method for increasing oil content of microalgae produced by culturing smoke Download PDFInfo
- Publication number
- CN114437935B CN114437935B CN202011198850.9A CN202011198850A CN114437935B CN 114437935 B CN114437935 B CN 114437935B CN 202011198850 A CN202011198850 A CN 202011198850A CN 114437935 B CN114437935 B CN 114437935B
- Authority
- CN
- China
- Prior art keywords
- microalgae
- culturing
- culture medium
- flue gas
- seed liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000012258 culturing Methods 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 26
- 239000000779 smoke Substances 0.000 title abstract description 10
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 claims abstract description 36
- 239000003546 flue gas Substances 0.000 claims abstract description 36
- 241000894006 Bacteria Species 0.000 claims abstract description 23
- 238000004321 preservation Methods 0.000 claims abstract description 14
- 230000005526 G1 to G0 transition Effects 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims description 29
- 239000001963 growth medium Substances 0.000 claims description 28
- 238000005286 illumination Methods 0.000 claims description 11
- 238000002360 preparation method Methods 0.000 claims description 6
- 241000589597 Paracoccus denitrificans Species 0.000 claims description 5
- 229910052799 carbon Inorganic materials 0.000 claims description 5
- 241000233866 Fungi Species 0.000 claims description 4
- 230000003698 anagen phase Effects 0.000 claims description 2
- 230000012010 growth Effects 0.000 claims description 2
- 241001057811 Paracoccus <mealybug> Species 0.000 claims 1
- 239000004519 grease Substances 0.000 abstract description 15
- 244000005700 microbiome Species 0.000 abstract description 6
- 230000002195 synergetic effect Effects 0.000 abstract description 3
- 238000006477 desulfuration reaction Methods 0.000 description 10
- 230000023556 desulfurization Effects 0.000 description 10
- 239000000243 solution Substances 0.000 description 9
- 239000000706 filtrate Substances 0.000 description 8
- 239000007789 gas Substances 0.000 description 7
- 241000195493 Cryptophyta Species 0.000 description 6
- 239000002028 Biomass Substances 0.000 description 4
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- OAYLNYINCPYISS-UHFFFAOYSA-N ethyl acetate;hexane Chemical compound CCCCCC.CCOC(C)=O OAYLNYINCPYISS-UHFFFAOYSA-N 0.000 description 3
- 238000004231 fluid catalytic cracking Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 238000007670 refining Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 238000009777 vacuum freeze-drying Methods 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- PFUVRDFDKPNGAV-UHFFFAOYSA-N sodium peroxide Chemical compound [Na+].[Na+].[O-][O-] PFUVRDFDKPNGAV-UHFFFAOYSA-N 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241001301789 Desmodesmus abundans Species 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 1
- 241000195663 Scenedesmus Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- FRHBOQMZUOWXQL-UHFFFAOYSA-L ammonium ferric citrate Chemical compound [NH4+].[Fe+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O FRHBOQMZUOWXQL-UHFFFAOYSA-L 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 238000009993 causticizing Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229960004642 ferric ammonium citrate Drugs 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000004313 iron ammonium citrate Substances 0.000 description 1
- 235000000011 iron ammonium citrate Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 229940074404 sodium succinate Drugs 0.000 description 1
- ZDQYSKICYIVCPN-UHFFFAOYSA-L sodium succinate (anhydrous) Chemical compound [Na+].[Na+].[O-]C(=O)CCC([O-])=O ZDQYSKICYIVCPN-UHFFFAOYSA-L 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000012224 working solution Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Abstract
The invention relates to a method for improving the grease content of microalgae for producing oil by culturing smoke, which comprises the following steps: (1) Introducing the flue gas into a photobioreactor to culture microalgae, wherein the microalgae are HCS-BY1 of the chain belt algaeDesmodesmus abundans) The preservation number is CGMCC No.19982; (2) Culturing until the stable phase is advanced, and inoculating denitrifying bacteria to continue culturing until the stable phase is finished. The invention cultures microalgae by utilizing flue gas, inoculates denitrifying bacteria before the stationary phase, and improves the grease content of the oil-producing microalgae by the synergistic effect of the two microorganisms.
Description
Technical Field
The invention belongs to the technical field of biomass energy, and particularly relates to a method for improving the grease content of microalgae produced by culturing smoke.
Background
The flue gas contains high-concentration carbon dioxide, so that the flue gas can be used for microalgae cultivation, and on one hand, CO is realized by carbon fixation of microalgae 2 Emission reduction, and microalgae for producing grease, starch and the like can be obtained.However, most of the flue gas contains harmful substances such as SOx, NOx and the like, and has an inhibition effect on carbon fixation and growth of microalgae. Researchers find that, in practical use, when CO is in the environment 2 When the volume fraction is more than 5v%, most microalgae are inhibited from growing, and the carbon fixation efficiency is low; and CO in industrially discharged gas 2 The concentration is generally 10% -20% and contains substances which have toxic effects on microalgae, such as SOx, NOx and the like. Therefore, microalgae are cultivated by using the flue gas, or CO in the flue gas needs to be removed or reduced in advance 2 Contaminants such as SOx and NOx, or the cultured microalgae need to be tolerant to high CO 2 Concentration, and resistance to harmful substances such as SOx and NOx.
CN109939548A discloses a flue gas desulfurization and denitration method, wherein flue gas is introduced into a desulfurization reactor for ammonia desulfurization to obtain an absorption liquid; introducing the desulfurization flue gas into a photobioreactor for culturing microalgae, and collecting exhaust gas, wherein the microalgae are NOx-tolerant microalgae; separating solid and liquid of the microalgae culture system, and respectively harvesting microalgae cells and filtrate; adding sodium peroxide into the filtrate, and introducing the collected exhaust gas into the filtrate to obtain purified gas; mixing the filtrate obtained by oxidation with desulfurization absorption liquid, and performing anaerobic ammoxidation treatment. The method combines the wet desulfurization and microalgae cultivation processes to treat the flue gas containing CO2, SO2 and NOx, realizes the efficient treatment of the flue gas, does not need to use a catalyst, and has the advantages of good removal effect, low treatment cost, economy, environmental protection and the like. However, since the microalgae are intolerant to SO 2 Therefore, the SO needs to be removed in advance 2 。
CN109939549a discloses a comprehensive treatment method and device for flue gas, the flue gas is led into a desulfurization reactor, and oil refining alkaline residue is used as absorbent to obtain desulfurized flue gas and desulfurized waste liquid; causticizing and regenerating the desulfurization waste liquid to obtain a precipitate, and reacting the obtained high-concentration SO2 with desulfurization flue gas to prepare sulfur; the generated gas is introduced into a photobioreactor for microalgae cultivation to collect exhaust gas; separating solid and liquid of the culture system, and respectively harvesting microalgae cells and filtrate; adding sodium peroxide into the filtrate, and introducing the exhaust gasCarrying out reaction in the filtrate to obtain purified gas; and (5) performing denitrification treatment on the filtrate after oxidation. The method is to culture and couple oil refining alkali residues and microalgae to treat SO-containing substances 2 、CO 2 And the high-efficiency treatment of the flue gas is realized by the flue gas of NOx. However, the invention employs microalgae that are only resistant to NOx, since microalgae are not resistant to SO 2 Therefore, the method needs to adopt the oil refining alkaline residue to remove SO in advance 2 。
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a method for improving the grease content of microalgae for producing oil by culturing smoke. The invention cultures microalgae by utilizing flue gas, inoculates denitrifying bacteria before the stationary phase, and improves the grease content of the oil-producing microalgae by the synergistic effect of the two microorganisms.
The invention provides a method for improving the grease content of microalgae for producing oil by culturing smoke, which comprises the following steps: (1) Introducing the flue gas into a photobioreactor to culture microalgae, wherein the microalgae are HCS-BY1 of the chain belt algaeDesmodesmus abundans) The preservation number is CGMCC No.19982; (2) Culturing until the stable phase is advanced, and inoculating denitrifying bacteria to continue culturing until the stable phase is finished.
In the invention, the chain belt alga HCS-BY is 1 #Desmodesmus abundans) Preserving in China general microbiological culture Collection center; the preservation number is CGMCC No.19982; preservation date: 5 months and 12 days 2020; preservation address: the institute of microorganisms of national academy of sciences of China, no.1, no.3, north Chen West Lu, the Korean region of Beijing. The chain belt algae can simultaneously resist SO 2 And NOx, the flue gas is utilized for culture without prior desulfurization and denitration.
In the invention, the flue gas is derived from at least one of FCC regenerated flue gas, coal-fired flue gas and the like, wherein the concentration of SOx is less than or equal to 0.05v%, the concentration of NOx is less than or equal to 0.07v%, and the concentration of CO is less than or equal to 0.07v% 2 The concentration is less than or equal to 40 percent by volume, the concentration of SOx is preferably 0.01 to 0.05 percent by volume, the concentration of NOx is 0.01 to 0.07 percent by volume, and the concentration of CO is preferably 0.01 to 0.07 percent by volume 2 The concentration is 5-30 v%.
In the invention, the photobioreactor is a reactor for culturing microalgae conventionally, and can perform light-dark alternate culture.
In the invention, microalgae seed liquid and a microalgae culture medium are added into a photobioreactor to culture microalgae, and the volume ratio of the microalgae seed liquid to the microalgae culture medium is 1:20-1:5. The microalgae culture medium adopts any one of BG11 culture medium, SE culture medium, D1 culture medium, etc. Specifically, the preparation of the culture medium and the microalgae seed liquid is determined according to the types of the microalgae, and the preparation is the same as that of the conventional method. The preparation method of the microalgae seed liquid comprises the following steps: inoculating microalgae into a microalgae culture medium, and culturing in an oscillating manner until the growth phase of the microalgae seed liquid is logarithmic under the conditions that the pH value is 7-9, the temperature is 20-35 ℃, the illumination period is 24 hours, the light-dark time ratio is 14:10 and the illumination intensity is 2000-20000 Lux, thereby obtaining the microalgae seed liquid.
In the invention, the conditions for culturing in the photobioreactor are as follows: the illumination intensity is 1500-20000 Lux, the pH value is 6-9, the temperature is 20-35 ℃, the light-dark period is 24 hours, and the light-dark time ratio is 14:10-10:14.
In the present invention, the culture is carried out until the period of stabilization is advanced, generally until the culture is carried out until the period of 48 to 72 inoculation of denitrifying bacteria. Culturing for 168-192 h until the later period of the stationary phase.
In the invention, the denitrifying bacteria are denitrifying bacteria capable of converting nitrate nitrogen and/or nitrite nitrogen into nitrogen. Preferably, paracoccus denitrificans DN-3 is adoptedParacoccus denitrificans) The preservation number is CGMCC No.3658, and the strain is disclosed in CN102465104A and submitted for preservation and survival certification.
In the invention, the preparation method of the denitrifying bacteria seed liquid comprises the following steps: the paracoccus denitrificans DN-3 on the plate is inoculated in the culture medium by a fungus inoculating loop, and is cultivated to the logarithmic phase at the temperature of 20-30 ℃ and the speed of 100-150 rpm. The volume ratio of the denitrifying bacteria seed liquid to the microalgae culture system is 1:20-50.
Compared with the prior art, the invention has the following beneficial effects:
(1) Aiming at the characteristics of industrial flue gas and the performance of the cultured microalgae, the flue gas is introduced into a photobioreactor for microalgae culture, denitrifying bacteria are inoculated in the early stage of the stationary phase of the culture, and the grease content of the oil-producing microalgae is improved through the synergistic effect of the two microorganisms.
(2) The chain belt algae provided by the invention can simultaneously resist SO 2 And NOx, so that pretreatment procedures are reduced, and the flue gas is recycled.
Detailed Description
The technical scheme and effects of the present invention are further described in detail below with reference to specific examples. The embodiments and specific operation procedures are given on the premise of the technical scheme of the invention, but the protection scope of the invention is not limited to the following embodiments.
The experimental methods in the following examples, unless otherwise specified, are all conventional in the art. The experimental materials used in the examples described below, unless otherwise specified, were purchased from conventional biochemical reagent stores. In the present invention, v% is the volume fraction.
The microalgae culture medium adopted in the embodiment of the invention is BG11 culture medium, and the formula is shown in Table 1 and Table 2.
TABLE 1 BG11 Medium
| Composition of the components | Working solution[g/L] |
| NaNO 3 | 1.5 |
| K 2 HPO 4 ·3H 2 O | 0.04 |
| MgSO 4 ·7H 2 O | 0.075 |
| CaCl 2 ·2H 2 O | 0.036 |
| Citric acid | 0.006 |
| Ferric ammonium citrate | 0.006 |
| EDTA | 0.001 |
| Na 2 CO 3 | 0.02 |
| A5+Co solution* | 1mL |
| Distilled water | 919 |
* Table 2 composition of A5+Co solution in Table 1
| Composition of the components | Content (g/L) |
| H 3 BO 3 | 2.86 |
| MnCl 2 ·H 2 O | 1.81 |
| ZnSO 4 ·7H 2 O | 0.222 |
| CuSO 4 ·5H 2 O | 0.079 |
| Na 2 MoO 4 ·2H 2 O | 0.390 |
| Co(NO 3 ) ·6H 2 O | 0.049 |
The chain belt alga HCY-BY1 is a new alga species separated and screened BY the inventor and is preserved in the common microorganism center of China Committee for culture Collection of microorganisms; preservation number: CGMCC No.19982; preservation date: 5 months and 12 days 2020; preservation address: the institute of microorganisms of national academy of sciences of China, no.1, no.3, north Chen West Lu, the Korean region of Beijing.
BG11 liquid media were first prepared according to tables 1 and 2, the pH of the media was adjusted to 8.0, and then microalgae were inoculated into the media, respectively. Culturing in a constant temperature illumination shaking table at 25deg.C for 24 hr with a light-dark time ratio of 14:10 and illumination intensity of 5000Lux at 120rpm to logarithmic phase to obtain microalgae seed liquid.
In the invention, the culture medium formula of the paracoccus denitrificans DN-3 is as follows: KNO (KNO) 3 1g/L sodium succinate 8g/L KH 2 PO 4 :1g/L，FeCl 2 0.5g/L; the solid medium was added with 20g/L agar. And inoculating the paracoccus denitrificans DN-3 on the flat plate into a culture medium by using a fungus inoculating loop, and performing shake culture for 48 hours to logarithmic phase at the temperature of 30 ℃ and at the speed of 150rpm to obtain a fungus seed solution.
The flue gas is derived from regenerated flue gas of FCC (fluid catalytic cracking) device of a certain refinery, wherein the concentration of SOx is 0.02-0.04% by volume, the concentration of NOx is 0.02-0.06% by volume, and the concentration of CO is 2 The concentration is 10-14 v%.
Example 1
Adding a seed solution of the chain belt alga HCY-BY1 and a microalgae culture medium into a 20L photobioreactor, wherein the adding amount of the seed solution is 800mL, the adding amount of the microalgae culture medium is 8L, and introducing smoke to perform light-dark alternate culture, wherein the concentration of SOx in the smoke is 0.03v%, the concentration of NOx is 0.05v% and the concentration of CO is 0.05v% 2 The concentration was 14v%. The light-dark period is 24 hours, the light-dark time ratio is 14:10, the illumination intensity is 5000Lux, the culture temperature is 20 ℃, and the pH value is 6-9.
Inoculating denitrifying bacteria seed liquid after alternate culturing for 72 hours in a light-dark mode, wherein the volume ratio of the denitrifying bacteria seed liquid to the microalgae culture system is 1:40, and finishing culturing after 168 hours of culturing.
Microalgae cells are obtained by centrifugation, and the dry weight and the grease content of the cells are measured. And (3) vacuum freeze-drying at-60 ℃ to constant weight, measuring the dry weight of the algae powder, calculating the biomass yield, and measuring the total fat content by adopting an n-hexane-ethyl acetate method. The detection shows that the dry cell weight is 12.3g/L and the grease content is 55.45% of the dry cell weight.
Example 2
Adding a seed solution of the chain belt alga HCY-BY1 and a microalgae culture medium into a 20L photobioreactor, wherein the adding amount of the seed solution is 400mL, the adding amount of the microalgae culture medium is 8L, and introducing smoke to perform light-dark alternate culture, wherein the concentration of SOx in the smoke is 0.02v%, the concentration of NOx is 0.06v%, and the concentration of CO is 0.06v% 2 The concentration was 13.5v%. The light-dark period is 24 hours, the light-dark time ratio is 14:10, the illumination intensity is 5000Lux, the culture temperature is 25 ℃, and the pH value is 6-9.
Inoculating denitrifying bacteria seed liquid after alternate culturing for 72 hours in a light-dark mode, wherein the volume ratio of the denitrifying bacteria seed liquid to the microalgae culture system is 1:30, and finishing culturing after culturing for 180 hours.
Microalgae cells are obtained by centrifugation, and the dry weight and the grease content of the cells are measured. And (3) vacuum freeze-drying at-60 ℃ to constant weight, measuring the dry weight of the algae powder, calculating the biomass yield, and measuring the total fat content by adopting an n-hexane-ethyl acetate method. The detection shows that the dry cell weight is 12.6g/L and the grease content is 53.51% of the dry cell weight.
Example 3
Adding seed solution of chain belt alga HCY-BY1 and microalgae into a 20L photobioreactorThe addition amount of the culture medium and the seed solution is 800mL, the addition amount of the microalgae culture medium is 8L, and the microalgae culture medium is introduced into flue gas for light-dark alternate culture, wherein the concentration of SOx in the flue gas is 0.04v%, the concentration of NOx is 0.03v% and the concentration of CO is 0.03v% 2 The concentration was 13v%. The light-dark period is 24 hours, the light-dark time ratio is 14:10, the illumination intensity is 8000Lux, the culture temperature is 30 ℃, and the pH value is 6-9.
Inoculating denitrifying bacteria seed liquid after culturing for 48 hours in a light-dark alternation mode, wherein the volume ratio of the denitrifying bacteria seed liquid to the microalgae culture system is 1:50, and finishing culturing after culturing for 168 hours.
Microalgae cells are obtained by centrifugation, and the dry weight and the grease content of the cells are measured. And (3) vacuum freeze-drying at-60 ℃ to constant weight, measuring the dry weight of the algae powder, calculating the biomass yield, and measuring the total fat content by adopting an n-hexane-ethyl acetate method. The detection shows that the dry cell weight is 11.7g/L and the grease content is 56.45% of the dry cell weight.
Example 4
The difference from example 1 is that: inoculating denitrifying bacteria SDN-3 described in CN102465103A in the culture process. The dry cell weight was found to be 8.5g/L and the oil content was found to be 45.33% of the dry cell weight.
Comparative example 1
The difference from example 1 is that: no denitrifying bacteria are inoculated in the culture process. The dry cell weight was found to be 7.9g/L and the fat content was found to be 35.21% of the dry cell weight.
Comparative example 2
The difference from example 1 is that: the microalgae adopts the fibrous algae SS-B7 disclosed in CN105713836A, and the preservation number is CGMCC No. 7478. The dry cell weight was found to be 7.3g/L and the fat content was found to be 36.75% of the dry cell weight.
Comparative example 3
The difference from example 1 is that: the microalgae adopts scenedesmus HY-D3 disclosed in CN111100796A, and the preservation number is CGMCC No. 15298. The detection shows that the dry weight of the cells is 4.8g/L, and the grease content is 30.5% of the dry weight of the cells.
Claims (7)
1. The method for improving the oil content of the microalgae produced by the flue gas culture is characterized by comprising the following steps of: (1) Introducing the flue gas into a photobioreactor to carry out microalgaeCulturing the microalgae which are chain belt algaeDesmodesmus abundans) HCS-BY1 with a preservation number of CGMCC No.19982; (2) Culturing until the stable phase is advanced, and inoculating denitrifying bacteria to continue culturing until the stable phase is finished; the flue gas is derived from at least one of FCC regenerated flue gas and coal-fired flue gas, wherein the concentration of SOx is 0.01-0.05 v%, the concentration of NOx is 0.01-0.07 v%, and the concentration of CO is 0.01-0.07 v% 2 Concentration is 5-30 v%; the denitrifying bacteria are Paracoccus denitrificansParacoccus denitrificans) DN-3 with the preservation number of CGMCC No. 3658.
2. The method according to claim 1, characterized in that: adding microalgae seed liquid and a microalgae culture medium into a photobioreactor to culture microalgae, wherein the volume ratio of the microalgae seed liquid to the microalgae culture medium is 1:20-1:5.
3. The method according to claim 1 or 2, characterized in that: the microalgae culture medium adopts any one of BG11 culture medium, SE culture medium and D1 culture medium.
4. The method according to claim 1 or 2, characterized in that: the preparation method of the microalgae seed liquid comprises the following steps: inoculating microalgae into a microalgae culture medium, and culturing in an oscillating manner until the growth phase of the microalgae seed liquid is logarithmic under the conditions that the pH value is 7-9, the temperature is 20-35 ℃, the illumination period is 24 hours, the light-dark time ratio is 14:10 and the illumination intensity is 2000-20000 Lux, thereby obtaining the microalgae seed liquid.
5. The method according to claim 1, characterized in that: the conditions for culturing in the photobioreactor are as follows: the illumination intensity is 1500-20000 Lux, the pH value is 6-9, the temperature is 20-35 ℃, the light-dark period is 24 hours, and the light-dark time ratio is 14:10-10:14.
6. The method according to claim 1, characterized in that: culturing until the stable phase is advanced, culturing for 48-72 h, and inoculating denitrifying bacteria; culturing for 150-192 h until the later period of the stationary phase.
7. The method according to claim 1, characterized in that: the preparation method of the denitrifying bacteria seed liquid comprises the following steps: inoculating the paracoccus denitrificans DN-3 on the flat plate into a culture medium by a fungus inoculating loop, and culturing at the temperature of 20-30 ℃ and at the speed of 100-150 rpm until the growth period of logarithm; the volume ratio of the denitrifying bacteria seed liquid to the microalgae culture system is 1:20-50.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011198850.9A CN114437935B (en) | 2020-10-31 | 2020-10-31 | Method for increasing oil content of microalgae produced by culturing smoke |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011198850.9A CN114437935B (en) | 2020-10-31 | 2020-10-31 | Method for increasing oil content of microalgae produced by culturing smoke |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114437935A CN114437935A (en) | 2022-05-06 |
| CN114437935B true CN114437935B (en) | 2023-07-04 |
Family
ID=81357728
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011198850.9A Active CN114437935B (en) | 2020-10-31 | 2020-10-31 | Method for increasing oil content of microalgae produced by culturing smoke |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114437935B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103160440A (en) * | 2011-12-19 | 2013-06-19 | 新奥科技发展有限公司 | Scenedesmus strain and application thereof |
| CN109576315A (en) * | 2017-09-28 | 2019-04-05 | 中国石油化工股份有限公司 | A method of microalgae grease is produced using flue gas |
| CN111100883A (en) * | 2018-10-26 | 2020-05-05 | 中国石油化工股份有限公司 | Method for producing microalgae grease by using flue gas |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8080679B2 (en) * | 2007-12-21 | 2011-12-20 | Old Dominion University Research Foundation | Direct conversion of biomass to biodiesel fuel |
-
2020
- 2020-10-31 CN CN202011198850.9A patent/CN114437935B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103160440A (en) * | 2011-12-19 | 2013-06-19 | 新奥科技发展有限公司 | Scenedesmus strain and application thereof |
| CN109576315A (en) * | 2017-09-28 | 2019-04-05 | 中国石油化工股份有限公司 | A method of microalgae grease is produced using flue gas |
| CN111100883A (en) * | 2018-10-26 | 2020-05-05 | 中国石油化工股份有限公司 | Method for producing microalgae grease by using flue gas |
Non-Patent Citations (5)
| Title |
|---|
| A novel CO 2 -tolerant symbiotic Desmodesmus (Chlorophyceae, Desmodesmaceae): Acclimation to and performance at a high carbon dioxide level;Alexei Solovchenko等;《Algal Research》;全文 * |
| NaCl as an effective inducer for lipid accumulation in freshwater microalgae Desmodesmus abundans;Ling Xia等;《Bioresource Technology》;全文 * |
| 一株高生物量链带藻分离筛选与培养条件优化;季方;郝睿;刘颖;李道义;周宇光;董仁杰;;农业机械学报(第S2期);全文 * |
| 微藻高CO_2耐受机制及其在生物减碳领域的应用;李凤娟;高大文;胡晗华;;哈尔滨工业大学学报(第04期);全文 * |
| 用混合氮源在室外培养产油链带藻的可行性研究;夏令;胡春香;;水生生物学报(第06期);全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114437935A (en) | 2022-05-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109576315B (en) | Method for producing microalgae grease by using flue gas | |
| CN104860474B (en) | Method for carbon sequestration and biological treatment of sulfur-containing waste alkali liquor | |
| CN102061261B (en) | Method for culturing microalgae by utilizing flue gas of coal fired power plant | |
| CN111100883B (en) | Method for producing microalgae grease by using flue gas | |
| CN110655199A (en) | Method for treating ammonia nitrogen wastewater by using heterotrophic nitrification-aerobic denitrification pseudomonas strain | |
| KR102564256B1 (en) | Microorganism producing sulfur and method for preparing sulfur using the same | |
| CN109576314B (en) | Method for preparing microalgae grease through mixed culture | |
| CN104857842B (en) | One plant of thermophilic salt basophilla sulfur oxidizing bacterium and its application in gaseous bio desulfurization-sulfur recovery | |
| CN111793573B (en) | Shewanella alga strain with functions of heterotrophic nitrate heterotrophic reduction and autotrophic nitrate heterotrophic reduction to ammonium, culture method and application thereof | |
| CN109939549B (en) | Comprehensive treatment method and device for flue gas | |
| CN109880856B (en) | Open type microalgae grease production method | |
| CN114437935B (en) | Method for increasing oil content of microalgae produced by culturing smoke | |
| CN112063550A (en) | For reducing Fe in complex stateⅢAnaerobic strain, culture method and application thereof | |
| CN109939560B (en) | Method and device for treating sulfur-containing flue gas | |
| CN114432869B (en) | Method and device for treating coal-fired flue gas | |
| CN109939548B (en) | Flue gas desulfurization and denitrification method and device | |
| CN111676176B (en) | Halophilic alkalophilic sulfur micro-spirillum, culture method thereof and application thereof in biological desulfurization | |
| CN111676169B (en) | Halophilic basophilic micro-oxysulfuricus bacterium for high yield of elemental sulfur and application thereof in biological desulfurization | |
| CN112695000B (en) | Strain for reducing complex state ferric iron and application thereof | |
| CN109876642B (en) | Method and device for treating flue gas containing nitrogen oxides | |
| CN112725388B (en) | Method for producing microalgae grease by using flue gas | |
| CN109876641B (en) | Method and device for treating NOx-containing flue gas | |
| CN109876643B (en) | Method and device for treating flue gas containing nitrogen oxides | |
| CN111996134A (en) | Halophilic and basophilic sulfur oxidation complex microbial inoculant, preparation method thereof and application thereof in biological desulfurization | |
| CN109876603B (en) | Method and device for treating sulfur-containing flue gas |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20231123 Address after: 100728 No. 22 North Main Street, Chaoyang District, Beijing, Chaoyangmen Patentee after: CHINA PETROLEUM & CHEMICAL Corp. Patentee after: Sinopec (Dalian) Petrochemical Research Institute Co.,Ltd. Address before: 100728 No. 22 North Main Street, Chaoyang District, Beijing, Chaoyangmen Patentee before: CHINA PETROLEUM & CHEMICAL Corp. Patentee before: DALIAN RESEARCH INSTITUTE OF PETROLEUM AND PETROCHEMICALS, SINOPEC Corp. |
|
| TR01 | Transfer of patent right |