CN111676169B

CN111676169B - Halophilic basophilic micro-oxysulfuricus bacterium for high yield of elemental sulfur and application thereof in biological desulfurization

Info

Publication number: CN111676169B
Application number: CN202010633804.0A
Authority: CN
Inventors: 杨茂华; 邢建民; 穆廷桢; 苗得露
Original assignee: Institute of Process Engineering of CAS
Current assignee: Institute of Process Engineering of CAS
Priority date: 2020-07-02
Filing date: 2020-07-02
Publication date: 2021-11-12
Anticipated expiration: 2040-07-02
Also published as: CN111676169A

Abstract

The invention relates to a halophilic basophilic micro oxysulfuricus bacterium for high yield of elemental sulfur and application thereof in biological desulfurization, wherein the halophilic basophilic micro oxysulfuricus bacterium for high yield of elemental sulfur is named as micro oxysulfur microspiromyces (Thiomicrospira microaerophila) BDL05 strain, the preservation unit is the common microorganism center of China Committee for culture Collection of microorganisms, the preservation time is 6 months and 10 days in 2020, the preservation number is CGMCC No.20060, and the addresses are as follows: xilu No. 1 Hospital No. 3, Beijing, Chaoyang, North. The halophilic basophilic micro oxysulfuricus BDL05 can oxidize hydrosulfide to generate elemental sulfur under the condition of high salt and high alkali, the yield of the elemental sulfur reaches more than 95 percent, the stability of a biological desulfurization system is obviously improved, and the application range of the biological desulfurization technology is expanded.

Description

Halophilic basophilic micro-oxysulfuricus bacterium for high yield of elemental sulfur and application thereof in biological desulfurization

Technical Field

The invention belongs to the technical field of microbial separation culture, particularly relates to a micro-oxysulfuricus bacterium and application thereof in biological desulfurization, and particularly relates to a halophilic basophilic micro-oxysulfuricus bacterium capable of producing elemental sulfur at a high yield and application thereof in biological desulfurization.

Background

The biological desulfurization technology can be used for removing H in gas at normal temperature and normal pressure₂S is removed and a simple substance sulfur product is generated. Compared with the traditional physical and chemical methods, the biological desulfurization technology has the advantages of mild conditions, no secondary pollution, no system blockage and the like, and is applied to desulfurization projects of natural gas, methane and the like at present. The desulfurization bacteria used in the technology are mainly chemoautotrophic microorganisms, and can utilize CO₂As a carbon source while oxidizing S^2-Obtain energy in the process. Hydrogen sulfide (H) in the gas to be treated₂S) is absorbed by alkali liquor in an absorption tower and converted into HS^-While entering the water phase, HS^-Adsorbed by desulfurization microorganisms into microbial cells in the presence of O₂Under the condition that the microorganism will convert S^2-Oxidizing into elemental sulfur, regenerating the alkali liquor and absorbing the hydrogen sulfide again.

In the biological desulfurization process, sulfur oxidizing bacteria can not only oxidize sulfide into elemental sulfur, but also inevitably oxidize a certain amount of elemental sulfur into sulfate. Peroxidation of elemental sulfur can cause various problems to a desulfurization system, such as reduction of yield of elemental sulfur; the pH value of the system is reduced, and the absorption efficiency of the hydrogen sulfide is reduced; the stability of the desulfurization system becomes poor, etc. Under the condition of controlling the optimal oxidation-reduction potential of sulfur oxidizing bacteria used at present, the yield of elemental sulfur is generally 80-85%, more than 10% of elemental sulfur is still converted into sulfate to enter a desulfurization system, and the long-term operation can cause system acidification and seriously affect the desulfurization efficiency. Therefore, the halophilic and basophilic desulfurization strains with high elemental sulfur yield are screened, so that the problem of systematic acidification in the field of biological desulfurization at present can be solved, and the technical performance and market acceptance of biological desulfurization are further improved.

Disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide a micro-oxysulfur micro-spirillum and application thereof in biological desulfurization, in particular to halophilic basophilic micro-oxysulfur micro-spirillum with high elemental sulfur yield and application thereof in biological desulfurization.

In order to achieve the purpose, the invention adopts the following technical scheme:

in a first aspect, the invention provides a halophilic basophilic micro oxysulfuricus bacterium for high elemental sulfur yield, which is named as micro oxysulfuricus bacterium (Thiomicrospira microaerophila) BDL05 strain, the preservation unit is China general microbiological culture Collection center, the preservation time is 2020, 6, month and 10 days, the preservation number is CGMCC No.20060, and the address is as follows: xilu No. 1 Hospital No. 3, Beijing, Chaoyang, North.

The high-yield elemental sulfur refers to that when the sulfur-containing gas or wastewater is treated by the sulfur oxysulfide micro-spirochete, the generation rate of elemental sulfur is more than 95%.

The microaerophilic sulfur-micro spirillum BDL05 strain related by the invention is separated from the sediment at the bank of the desert saline-alkali lake of inner Mongolia badam and has the characteristic of oxidizing sulfide to generate elemental sulfur under the conditions of high salt and high alkali. The strain is chemoautotrophic microorganism, the cell energy is derived from chemical energy generated by oxidation of reduced sulfur-containing compounds such as hydrosulfide, elemental sulfur, sodium thiosulfate, etc., and the carbon source is derived from carbon dioxide in atmosphere, and can be pH8.0-12.0, 0.5-4.0M Na⁺And the growth is carried out under the conditions of high salt and high alkali.

The invention relates to a method for separating and culturing a Microspirillum oxysulfide BDL05 strain, which comprises the following steps:

(1) collecting sediments beside a saline-alkali lake of a desert of inner Mongolia badan Jilin, putting 1g of the sediments in a 100mL liquid culture medium of sodium thiosulfate, and carrying out shaking culture on a shaking table at 30 ℃ and 150rpm for 60 hours;

(2) inoculating 1mL of the suspension into 100mL of sodium thiosulfate liquid culture medium, and continuing shaking culture at 30 ℃ and 150rpm for 60 hours by a shaking table;

(3) coating 100 mu L of suspension on the surface of a sodium thiosulfate solid culture medium, standing and culturing at 30 ℃ for 60h, and selecting white colonies for re-screening and identification;

wherein the sodium thiosulfate liquid medium includes, for exampleThe following components: 30g/L Na₂S₂O₃、30g/L NaHCO₃、5g/L NaOH、10g/L NaCl、0.5g/L NH₄Cl、0.5g/L KNO₃、2.0g/L K₂HPO₄、0.1g/L MgCl₂。

Wherein, the other components in the sodium thiosulfate solid culture medium are as follows: 30g/L Na₂S₂O₃、30g/L NaHCO₃、5g/L NaOH、10g/L NaCl、0.5g/L NH₄Cl、0.5g/L KNO₃、2.0g/L K₂HPO₄、0.1g/L MgCl₂1.5% agar powder;

after multiple purifications, a micro-aerobic sulfur micro-spirillum BDL05 capable of producing elemental sulfur is obtained.

In a second aspect, the invention provides a method for culturing high-yield elemental sulfur-producing halophilic microspiromyces oxysporum, which comprises culturing the high-yield elemental sulfur-producing halophilic microspiromyces oxysporum in a medium containing sodium thiosulfate.

Preferably, the sodium thiosulfate-containing medium comprises Na₂S₂O₃、NaHCO₃、NH₄Cl、KNO₃、K₂HPO₄And MgCl₂。

Preferably, the medium containing sodium thiosulfate also comprises NaOH and/or NaCl.

Preferably, the culture medium containing sodium thiosulfate comprises 10-30g/L of Na in mass concentration₂S₂O₃、30-60g/L NaHCO₃、0-30g/L NaOH、0-100g/L NaCl、0.1-1.0g/L NH₄Cl、0.1-1.0g/L KNO₃、0.5-5.0g/L K₂HPO₄And 0.05-0.3g/L MgCl₂。

The Na is₂S₂O₃The mass concentration of the organic acid can be 10g/L, 15g/L, 20g/L, 25g/L or 30g/L, and the like, and specific point values in the numerical value range can be selected, so that the description is omitted.

Said NaHCO₃The mass concentration of (b) can be 30g/L, 35g/L, 40g/L, 45g/L, 50g/L, 55g/L or 60g/L, etcThe specific point values in the range can be selected, and are not described in detail herein.

The mass concentration of the NaOH can be 5g/L, 10g/L, 15g/L, 20g/L, 25g/L or 30g/L, and the like, specific point values in the numerical value range can be selected, and are not repeated here, and when the mass concentration is 0, the NaOH is not added.

The mass concentration of NaCl can be 5g/L, 10g/L, 20g/L, 30g/L, 40g/L, 50g/L, 60g/L, 80g/L or 100g/L, and the like, specific point values in the numerical value range can be selected, and are not repeated one by one, and when the mass concentration is 0, NaCl is not added.

The NH₄The mass concentration of Cl can be 0.1g/L, 0.2g/L, 0.3g/L, 0.4g/L, 0.5g/L, 0.6g/L, 0.7g/L, 0.9g/L or 1.0g/L, and the like, and specific point values in the numerical value range can be selected, so that the details are not repeated.

The KNO₃The mass concentration of the organic acid can be 0.1g/L, 0.2g/L, 0.3g/L, 0.4g/L, 0.5g/L, 0.6g/L, 0.7g/L, 0.9g/L or 1.0g/L, and the like, and specific point values in the numerical value range can be selected, so that the description is omitted.

Said K₂HPO₄The mass concentration of the organic acid can be 0.5g/L, 0.6g/L, 0.8g/L, 1.0g/L, 1.5g/L, 2.0g/L, 3.0g/L, 4.0g/L or 5.0g/L, and the like, and specific point values in the numerical value range can be selected, so that the description is omitted.

Said MgCl₂The mass concentration of the organic acid can be 0.05g/L, 0.1g/L, 0.15g/L, 0.2g/L, 0.25g/L or 0.3g/L, and the like, and specific point values in the numerical value range can be selected, so that the repeated description is omitted.

Preferably, the pH of the medium containing sodium thiosulfate is 8.0 to 12.0, for example, 8.0, 9.0, 10.0, 11.0 or 12.0, and the sodium ion concentration is 0.5 to 4.0M, for example, 0.5M, 1.0M, 1.5M, 2.0M, 2.5M, 3.0M or 4.0M, and the specific values in the above numerical range can be selected, which is not repeated herein.

In a third aspect, the invention provides an application of halophilic basophilic micro sulfur-oxygen micro spirillum with high elemental sulfur yield in biological desulfurization of hydrogen sulfide-containing gas.

In the present invention, the method of application comprises the steps of:

(1) absorbing hydrogen sulfide in the gas by using an alkaline washing solution to form a high-sulfur solution;

(2) and (2) inoculating the halophilic basophilic micro oxysulfur micro spirillum with high elemental sulfur yield into the high sulfur-containing solution obtained in the step (1) for desulfurization treatment.

In the desulfurization treatment process, sodium hydrosulfide is oxidized into elemental sulfur by microaspirillum oxysulfide to generate sodium hydroxide; the elemental sulfur is agglomerated to form micron-sized sulfur particles, naturally settled for 0.1min-2h and then discharged from the bioreactor, so as to achieve the aim of desulfurization; the lye discharged from the sulfur particles will continue to be recycled for absorption of hydrogen sulfide in the gas. The generation rate of elemental sulfur is more than 95 percent, and the removal rate of hydrogen sulfide reaches more than 99 percent.

Preferably, the gas comprises natural gas, biogas, petroleum refinery gas, coal refinery gas, landfill gas or viscose production waste gas.

Preferably, the alkaline washing solution contains any one or a combination of at least two of sodium carbonate, sodium bicarbonate or sodium hydroxide; the combination of at least two of the above-mentioned compounds, such as the combination of sodium carbonate and sodium bicarbonate, the combination of sodium bicarbonate and sodium hydroxide, etc., can be selected in any other combination manner, and thus, the details are not repeated herein.

Preferably, the pH of the alkaline washing solution is 8.0-12.0, for example, 8.0, 9.0, 10.0, 11.0 or 12.0, and the sodium ion concentration is 0.5-4.0M, for example, 0.5M, 1.0M, 1.5M, 2.0M, 2.5M, 3.0M or 4.0M, and the specific values in the above-mentioned value range can be selected, which is not repeated herein.

Preferably, the concentration of sodium hydrosulfide in the high sulfur-containing solution is 1.0-3.0g/L, such as 1.0g/L, 1.5g/L, 2.0g/L, 2.5g/L or 3.0g/L, and the like, and specific values within the above numerical value range can be selected, and are not repeated herein.

Preferably, the inoculation amount of the halophilic basophilic microspiromyces for high yield of elemental sulfur is 1-5%, for example, 1%, 2%, 3%, 4%, or 5%, and the specific values in the above numerical range can be selected, and are not described in detail herein.

In a fourth aspect, the invention provides an application of the halophilic basophilic micro sulfur-oxygen micro spirillum with high elemental sulfur yield in biological desulfurization of alkaline wastewater containing sulfide.

In the present invention, the method of application comprises the steps of:

the halophilic alkalophilic micro-oxysulfurin microspira bacteria with high elemental sulfur yield are inoculated into alkaline wastewater containing sulfide for desulfurization treatment.

In the desulfurization treatment process, the sulfur compound is oxidized into elemental sulfur by micro sulfur-oxidizing micro spirillum, and meanwhile, alkali is regenerated; the elemental sulfur is agglomerated to form micron-sized sulfur particles, naturally settled for 0.1min-2h and then discharged from the bioreactor, so that the aim of desulfurization is fulfilled, and the generation rate of the elemental sulfur is more than 95%.

Preferably, the sulfide-containing alkaline wastewater comprises leather wastewater, petroleum refining wastewater, pesticide production wastewater or synthetic ammonia wastewater.

Preferably, the pH of the sulfide-containing alkaline wastewater is 8.0 to 12.0, for example, 8.0, 9.0, 10.0, 11.0 or 12.0, and the concentration of sodium ions is 0.5 to 4.0M, for example, 0.5M, 1.0M, 1.5M, 2.0M, 2.5M, 3.0M or 4.0M, and the like, and specific values within the above-mentioned value range can be selected, which is not described in detail herein.

Preferably, the concentration of sulfide in the sulfide-containing alkaline wastewater is 0.1-3.0g/L, such as 0.1g/L, 0.5g/L, 1.0g/L, 2.0g/L, 2.5g/L or 3.0g/L, and the like, and specific values in the above numerical value range can be selected, and are not repeated herein.

Preferably, the oxidation-reduction potential of the sulfide-containing alkaline wastewater is-150 mV to-400 mV, such as-150 mV, -200mV, -250mV, -300mV, -350mV or-400 mV, and the like, and specific values in the above numerical value ranges can be selected, and are not repeated herein.

Preferably, the adjustment of the oxidation-reduction potential of the sulfide-containing alkaline wastewater comprises adjusting the supply of oxygen.

Compared with the prior art, the invention has the following beneficial effects:

the halophilic basophilic micro oxysulfuricus (Thiomicrospira microaerophila BDL05) capable of producing the elemental sulfur at high yield can oxidize hydrosulfide to generate the elemental sulfur under the condition of high salt and high alkali, the yield of the elemental sulfur reaches more than 95%, the stability of a biological desulfurization system is remarkably improved, and the application range of the biological desulfurization technology is expanded.

Drawings

FIG. 1 is a colony morphology of high elemental sulfur-producing halophilic microaerophila (Thiomicrospira microaerophila BDL05) according to the present invention; the preservation unit of the bacterial colony is China general microbiological culture Collection center, the preservation time is 6 months and 10 days in 2020, the preservation number is CGMCC No.20060, and the address is as follows: xilu No. 1 Hospital No. 3, Beijing, Chaoyang, North.

FIG. 2 is a gram stain of a high elemental sulfur-yielding halophilic Microthiospira microopilus (Thiomicrospira microaerophila BDL05) according to the present invention;

FIG. 3 is a map of the evolution of the halophilic basophilic Microthiospira (Thiomicrospira microaerophila BDL05) with high elemental sulfur yield according to the present invention;

FIG. 4 is a graph of the growth and metabolism of high elemental sulfur-yielding halophilic microaerophilus (Thiomicrospira microaerophila BDL05) according to the present invention;

FIG. 5 is a graph showing the change with time of the sulfide removal rate and the elemental sulfur production rate of a high-yield elemental sulfur-producing halophilic microaerophila BDL05 used in desulfurization treatment according to the present invention.

Detailed Description

The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.

Example 1

The present example provides a method for screening and separating halophilic basophilic microspira BDL05 with high elemental sulfur yield, which is performed as follows:

wherein, the sodium thiosulfate liquid culture medium comprises the following components: 30g/L Na₂S₂O₃、30g/L NaHCO₃、5g/L NaOH、10g/L NaCl、0.5g/L NH₄Cl、0.5g/L KNO₃、2.0g/L K₂HPO₄、0.1g/L MgCl₂。

The colony morphology of the obtained microaspirillum BDL05 was observed, as shown in fig. 1: the colony is white, round-like and irregular in edge, and the white colony gradually becomes transparent in the later culture period.

The obtained Microspirillum oxysporum BDL05 was gram-stained and negative, as shown in FIG. 2. The evolutionary position of Microspirillum oxysulphatum BDL05 is shown in FIG. 3.

The obtained Microspirillum microaerophilum BDL05 was subjected to 16S rDNA sequence determination, and the sequence was as follows:

GAAGTGCGCAGCTACCATGCAGTCGAACGGTAACAGAAGAGCTTGCTCTTGCTGACGAGTGGCGGACGGGTGAGTAATGCATAGGAATCTGCCCTCTAGTTGGGGATACCGTAGGGAAACTTACGTTAATACCGAATAGTCTCTAAGGAGTAAAGGTGGCCTCTACTTGTAAGCTATCGCTAGAGGATGAGCCTATGTTAGATTAGCTAGTTGGTGAGGTAATGGCTCACCAAGGCGACGATCTATAGCTGGTTTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCCGAGGGTTGTAAAGCACTTTTAGCGAGGAGGAAAGGATGTAGATTAATACCCTGCATCTGTGACGTTACTCGCAGAAAAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTATTCGGAATTACTGGGCGTAAAGCGCGCGTAGGCGGATTGTTAAGTCAGTTGTGAAAGCCCTGGGCTCAACCTAGGAACGGCGATTGAAACTGGCAATCTAGAGTTTAGTAGAGGGAAGGGGAATTTCTGGAGTAGCAGTGAAATGCGTAGATATCAGAAGGAACATCAGTGGCGAAGGCGCCTTCCTGGACTAAAACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACGGGATTAGATACCCCGGTAGTCCACGCCCTAAACGATGTCAACTAGCTGTTGGTCTTATTAAAAAGATTAGTAGCGCAGCTAACGCGATAAGTTGACCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCATCCCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAGCTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCATTAG。

the above analysis results indicate that it is a Thiomicrospira microaerophila (Thiomicrospira microaerophila).

Example 2

This example explores the characteristics of the growth of Microspirillum oxysporum BDL05 using sodium thiosulfate and operates as follows:

after streak activation culture of the strain of the sulfur micro spirillum BDL05 preserved at 4 ℃, inoculating the strain into 100mL of sodium thiosulfate liquid culture medium, placing the strain in a shaking table at 30 ℃ and 160r/min for 30h, and entering a logarithmic phase to be used as seed liquid for later use. Inoculating the strain in 100mL of sodium thiosulfate liquid medium at an inoculation amount of 2%, sampling at regular intervals, and determining S₂O₃ ^2-Concentration, SO₄ ^2-Concentration and OD values were varied with time.

Growth curves and S of the culture of Strain BDL05₂O₃ ^2-And SO₄ ^2-The change in concentration is shown in FIG. 4, and it can be seen from FIG. 4 that: the growth of the strain is slower in the first 7h under the conditions of 30 ℃ and 160 r/min; after the adaptation adjustment period, the strain BDL05 rapidly grows, the OD of the thallus of the culture solution reaches 0.6 at 32h, and then the OD value begins to decrease; s₂O₃ ^2-The concentration was initially 44mmol/L, S as the incubation time was extended₂O₃ ^2-The concentration is continuously reduced and is reduced to 0.85mmol/L in 32 h; SO (SO)₄ ^2-The concentration gradually increases with the culture time, and SO is added until the concentration reaches 40h₄ ^2-The concentration reaches 70.3 mmol/L. The sulfur utilization rate was 1.34 mmol/L/h.

It was described that BDL05 can be grown using sodium thiosulfate, and the above sodium thiosulfate liquid medium can be used as a culture solution for culturing BDL 05.

Example 3

This example uses Microspirillum oxysulphide BDL05 for the treatment of a hydrogen sulphide-containing gas, operating as follows:

(1) a test hydrogen sulfide-containing gas containing 6000ppm hydrogen sulfide and the balance nitrogen was prepared.

(2) The gas containing hydrogen sulfide flows through an absorption tower in a countercurrent way, during the process, the gas is contacted with alkali liquor, wherein the hydrogen sulfide is absorbed by the alkali liquor to form high-sulfur solution containing high sodium hydrosulfide; the high-sulfur solution enters a bioreactor; in a bioreactor, oxidizing sulfur hydride into elemental sulfur by microaspirillum BDL05 by controlling oxidation-reduction potential, and simultaneously regenerating alkali liquor for recycling;

wherein the alkali solution is an aqueous solution containing sodium carbonate, sodium bicarbonate or sodium hydroxide, the pH value is 9.7, and the sodium ion concentration is 3.0M; the concentration of sodium hydrosulfide in the high-sulfur solution is 2 g/L. In the biological sulfur oxidation process, the oxidation-reduction potential is controlled to be about-380 mv by controlling the sulfur oxidation products through the aeration quantity.

Through calculation, the generation rate of the elemental sulfur reaches more than 95 percent; the content of hydrogen sulfide in the purified gas is lower than 10ppm, and the removal rate of the hydrogen sulfide reaches more than 99%. The micro sulfur oxysulfide micro spirillum BDL05 has high-efficiency sulfur oxidizing capability, and the indexes of elemental sulfur generation rate, hydrogen sulfide removal rate and the like reach or exceed the existing strains, and can be applied to the treatment of the gas containing hydrogen sulfide.

Example 4

In this example, the operation of using the Microspirillum oxysulfide BDL05 for the treatment of alkaline sulfide wastewater is as follows:

(1) the wastewater for the preparation test is wastewater of a depilation section of a tanning enterprise, wherein the sodium sulfide reaches 2500mg/L, the pH value is 11.0, and the sodium ion concentration reaches 2.5M.

(2) Filtering the wastewater to remove substances such as large fat and the like; then adding the microspiromyces oxysulfuricus BDL05 seed liquid into sulfur-containing wastewater in an inoculation amount of 5%; by regulating and controlling aeration quantity and controlling the oxidation-reduction potential to be about-350 mv, the sulfur hydride is oxidized into elemental sulfur by the microaspirillum BDL05, and the elemental sulfur is separated from the wastewater through the sedimentation effect. The experiment is continued for 15 days, and the sulfide concentration and the elemental sulfur yield in the wastewater are uninterruptedly determined.

As shown in FIG. 5, the elemental sulfur yield was 95% or more; the content of sodium sulfide in the purified wastewater is lower than 25mg/L, and the removal rate of the sodium sulfide reaches more than 99%. The micro sulfur oxysulfide micro spirillum BDL05 has high-efficiency sulfur oxidizing capability, and the indexes of elemental sulfur generation rate, sulfide removal rate and the like reach or exceed the prior strains, and can be applied to the treatment of the wastewater containing the sulfide.

The applicant states that the present invention is illustrated by the above examples, but the present invention is not limited to the above examples, i.e. it does not mean that the present invention must rely on the above examples to be implemented. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.

The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are within the protective scope of the present invention.

It should be noted that the various technical features described in the above embodiments can be combined in any suitable manner without contradiction, and the invention is not described in any way for the possible combinations in order to avoid unnecessary repetition.

SEQUENCE LISTING

<110> institute of Process engineering of Chinese academy of sciences

<120> halophilic basophilic micro oxysulfuricus bacterium for high yield of elemental sulfur and application thereof in biological desulfurization

<130> 2020

<160> 1

<170> PatentIn version 3.3

<210> 1

<211> 1084

<212> DNA

<213> Microspira microaerophila (Thiomicrospira microaerophila) BDL05

<400> 1

gaagtgcgca gctaccatgc agtcgaacgg taacagaaga gcttgctctt gctgacgagt 60

ggcggacggg tgagtaatgc ataggaatct gccctctagt tggggatacc gtagggaaac 120

ttacgttaat accgaatagt ctctaaggag taaaggtggc ctctacttgt aagctatcgc 180

tagaggatga gcctatgtta gattagctag ttggtgaggt aatggctcac caaggcgacg 240

atctatagct ggtttgagag gatgatcagc cacactggga ctgagacacg gcccagactc 300

ctacgggagg cagcagtggg gaatattgga caatgggcgc aagcctgatc cagccatgcc 360

gcgtgtgtga agaaggcccg agggttgtaa agcactttta gcgaggagga aaggatgtag 420

attaataccc tgcatctgtg acgttactcg cagaaaaagc accggctaac tctgtgccag 480

cagccgcggt aatacagagg gtgcaagcgt tattcggaat tactgggcgt aaagcgcgcg 540

taggcggatt gttaagtcag ttgtgaaagc cctgggctca acctaggaac ggcgattgaa 600

actggcaatc tagagtttag tagagggaag gggaatttct ggagtagcag tgaaatgcgt 660

agatatcaga aggaacatca gtggcgaagg cgccttcctg gactaaaact gacgctgagg 720

tgcgaaagcg tggggagcaa acgggattag ataccccggt agtccacgcc ctaaacgatg 780

tcaactagct gttggtctta ttaaaaagat tagtagcgca gctaacgcga taagttgacc 840

gcctggggag tacggtcgca agattaaaac tcaaaggaat tgacgggggc ccgcacaagc 900

ggtggagcat gtggtttaat tcgatgcaac gcgaagaacc ttaccatccc ttgacatcca 960

cagaactttc cagagatgga ttggtgcctt cgggagctgt gagacaggtg ctgcatggct 1020

gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca acccctatca 1080

ttag 1084

Claims

1. The halophilic basophilic micro oxysulfuricus bacterium for high yield of elemental sulfur is named as micro oxysulfuricus bacterium (micro oxysulfuricus bacterium)Thiomicrospira microaerophila) The BDL05 strain has the preservation unit of China general microbiological culture Collection center, the preservation time of 6 months and 10 days in 2020, the preservation number of CGMCC No.20060 and the address of: xilu No. 1 Hospital No. 3, Beijing, Chaoyang, North.

2. The method for culturing the high elemental sulfur-yielding halophilic microspiromyces oxysulphur according to claim 1, wherein said method comprises culturing said high elemental sulfur-yielding halophilic microspiromyces oxysulphur in a medium containing sodium thiosulphate.

3. The method for culturing the high elemental sulfur yielding halophilic microaerophilus thiospiraea as claimed in claim 2, wherein said sodium thiosulfate-containing medium comprises Na₂S₂O₃、NaHCO₃、NH₄Cl、KNO₃、K₂HPO₄And MgCl₂。

4. The method for culturing high elemental sulfur yielding microorganisms of the halophilic basophilic sulfur microaspirillum according to claim 3, wherein said medium containing sodium thiosulfate further comprises NaOH and/or NaCl.

5. The method for culturing high elemental sulfur yielding Alcaligenes microsilica as claimed in claim 3, wherein said sodium thiosulfate-containing medium comprises 10-30g/LNa in terms of mass concentration₂S₂O₃、30-60g/L NaHCO₃、0-30g/L NaOH、0-100g/L NaCl、0.1-1.0g/L NH₄Cl、0.1-1.0g/L KNO₃、0.5-5.0g/L K₂HPO₄And 0.05-0.3g/L MgCl₂。

6. The method for culturing the high elemental sulfur yield halophilic basophilic microspiromyces oxysporum according to claim 2, wherein the pH value of the medium containing sodium thiosulfate is 8.0 to 12.0, and the sodium ion concentration is 0.5 to 4.0M.

7. The use of the halophilic basophilic microspiromyces oxysulfuricus for producing elemental sulfur in high yield according to claim 1 for biological desulfurization of a hydrogen sulfide-containing gas.

8. The application of claim 7, wherein the method of applying comprises the steps of:

(2) the method comprises the step of inoculating the halophilic basophilic micro oxysulfur micro spirillum with high elemental sulfur yield of claim 1 into the high sulfur-containing solution obtained in the step (1) for desulfurization treatment.

9. The use of claim 8, wherein the gas comprises natural gas, biogas, petroleum refinery gas, coal refinery gas, landfill gas, or viscose production waste gas.

10. Use according to claim 8, wherein the alkaline washing solution comprises any one or a combination of at least two of sodium carbonate, sodium bicarbonate or sodium hydroxide.

11. The use according to claim 8, wherein the alkaline washing solution has a pH of 8.0 to 12.0 and a sodium ion concentration of 0.5 to 4.0M.

12. The use of claim 8, wherein the concentration of sodium hydrosulfide in said high sulfur-containing solution is between 1.0 and 3.0 g/L.

13. The use according to claim 8, wherein said high yield of elemental sulphur is achieved by inoculating a quantity of microspira halophilic and basophilic microspira in the range of 1-5%.

14. The use of the halophilic basophilic microspiromyces oxysulfuricus for producing high elemental sulfur yield of claim 1 for biological desulfurization of sulfide-containing alkaline wastewater.

15. The application of claim 14, wherein the method of applying comprises the steps of:

the method comprises the step of inoculating the halophilic basophilic micro sulfur-oxygen micro spirillum capable of producing high-yield elemental sulfur in the sulfur-containing alkaline wastewater for desulfurization treatment.

16. The use of claim 15, wherein the sulfide-containing alkaline wastewater comprises leather wastewater, petroleum refining wastewater, pesticide production wastewater, or synthetic ammonia wastewater.

17. The use according to claim 15, wherein the sulfide-containing alkaline wastewater has a pH of 8.0 to 12.0 and a sodium ion concentration of 0.5 to 4.0M.

18. The use of claim 15, wherein the sulfide concentration in the sulfide-containing alkaline wastewater is from 0.1 to 3.0 g/L.

19. The use according to claim 15, wherein said high yield of elemental sulphur is achieved by inoculating a quantity of microspira halophilic basophilus in the range of 1-5%.

20. The use according to claim 15, characterized in that the oxide-reduction potential of the sulfide-containing alkaline waste water is between-150 and-400 mV.

21. The use according to claim 15, wherein the adjustment of the redox potential of the sulfide-containing alkaline wastewater comprises adjusting the amount of oxygen supplied.