CN114433319B - Tumor organ separation treatment method - Google Patents
Tumor organ separation treatment method Download PDFInfo
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- CN114433319B CN114433319B CN202210120832.1A CN202210120832A CN114433319B CN 114433319 B CN114433319 B CN 114433319B CN 202210120832 A CN202210120832 A CN 202210120832A CN 114433319 B CN114433319 B CN 114433319B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C18/00—Disintegrating by knives or other cutting or tearing members which chop material into fragments
- B02C18/06—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives
- B02C18/08—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives within vertical containers
- B02C18/10—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives within vertical containers with drive arranged above container
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C18/00—Disintegrating by knives or other cutting or tearing members which chop material into fragments
- B02C18/06—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives
- B02C18/16—Details
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C18/00—Disintegrating by knives or other cutting or tearing members which chop material into fragments
- B02C18/06—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives
- B02C18/16—Details
- B02C18/18—Knives; Mountings thereof
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C18/00—Disintegrating by knives or other cutting or tearing members which chop material into fragments
- B02C18/06—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives
- B02C18/16—Details
- B02C18/22—Feed or discharge means
- B02C18/2216—Discharge means
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C18/00—Disintegrating by knives or other cutting or tearing members which chop material into fragments
- B02C18/06—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives
- B02C18/16—Details
- B02C18/24—Drives
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C23/00—Auxiliary methods or auxiliary devices or accessories specially adapted for crushing or disintegrating not provided for in preceding groups or not specially adapted to apparatus covered by a single preceding group
- B02C23/08—Separating or sorting of material, associated with crushing or disintegrating
- B02C23/10—Separating or sorting of material, associated with crushing or disintegrating with separator arranged in discharge path of crushing or disintegrating zone
- B02C23/12—Separating or sorting of material, associated with crushing or disintegrating with separator arranged in discharge path of crushing or disintegrating zone with return of oversize material to crushing or disintegrating zone
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C23/00—Auxiliary methods or auxiliary devices or accessories specially adapted for crushing or disintegrating not provided for in preceding groups or not specially adapted to apparatus covered by a single preceding group
- B02C23/08—Separating or sorting of material, associated with crushing or disintegrating
- B02C23/14—Separating or sorting of material, associated with crushing or disintegrating with more than one separator
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- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Centrifugal Separators (AREA)
- Mixers Of The Rotary Stirring Type (AREA)
Abstract
The invention provides a tumor organ separation treatment method, which comprises a step-by-step separation and collection method, wherein multistage treatment equipment is adopted, and the tumor organ separation treatment method comprises a base (10), a positioning bracket (20), a mounting bracket (30), a fixing plate (40), a separation assembly (50), a sliding assembly (60) and a driving assembly (70); the separation assembly (50) comprises a primary separator (51), a secondary separator (52), a tertiary separator (53) and a stirring assembly (54), the sliding assembly (60) comprises a sliding rod (61), a screw rod (62), a connecting rod (63), a discharging rod (64) and a first motor (65), and the driving assembly (70) comprises a second motor (71), a rotating rod (72), a first gear (75), a second gear (74) and a third gear (73). The method does not need to change equipment and cutting tools, can realize step-by-step cutting and efficiently realize step-by-step release of the cell suspension.
Description
Technical Field
The invention relates to the technical field of medical treatment, in particular to a tumor organ separation treatment method.
Background
Clinical medicine is a discipline for diagnosing and treating problems (questions, discomfort or diseases related to the body or mind) of patients based on basic medicine; it encompasses clinical diagnostics, clinical therapeutics, and the like.
When clinically separating and treating tissues such as tumors, the tissues are generally required to be simply chopped, then subjected to tissue digestion by mechanical separation, enzyme treatment and other methods, and filtered to a certain extent to finally obtain cell clusters or cell suspensions; when the separation process is carried out, different equipment is generally required to be used for cutting and separating tissues, the equipment is complicated to replace and low in efficiency, and tissue pollution is easily caused in the equipment replacement process. Meanwhile, the cutting speed and the cutting force are often different for different tissue sizes, such as: the rough cutting of the tissue requires higher cutting speed or higher cutting force, thereby ensuring the breaking degree of the tissue; the tissue is subdivided into small groups, and then a softer cutting force or a lower cutting speed is needed, so that cell damage is avoided, in the prior art, aiming at different cutting speeds or cutting forces, the tissue is usually realized by replacing a cutter, the cutting speed is further reduced, the tissue is contacted with the external environment to cause tissue pollution, and the problems of inaccurate clinical detection results, delayed treatment and the like are caused.
Disclosure of Invention
Aiming at the problems existing in the prior art, the invention aims to provide a tumor organ separation treatment method which can realize step-by-step cutting and graded release of cell suspension without equipment replacement or cutting tool replacement, thereby obtaining cell suspensions with different tissue mass sizes and effectively avoiding the pollution caused by the contact of tissues with the external environment in the cutting process; meanwhile, the method can provide different cutting rates for tissues with different sizes, so that not only can the effective cutting and crushing degree of large tissue masses be ensured, but also small tissue masses can be effectively protected and prevented from being crushed, thereby realizing the collection of the tissue masses with different sizes and ensuring the comprehensive coverage and accuracy of clinical detection data.
The aim of the invention is achieved by the following technical scheme:
a tumor organ separation treatment method is characterized in that: the device comprises a step-by-step separation and collection method, wherein the step-by-step separation and collection are performed by adopting multi-stage treatment equipment, and the multi-stage treatment equipment comprises a base, a positioning bracket, a mounting bracket, a fixed plate, a separation assembly, a sliding assembly and a driving assembly; the bottom of the positioning bracket is fixedly connected with one side of the upper end surface of the base, and one end, far away from the base, of the positioning bracket is fixedly connected with the mounting bracket; the middle part of the positioning bracket is fixedly provided with a fixed plate, and the middle part of the fixed plate is provided with a through hole;
the separation assembly comprises a primary separator, a secondary separator, a tertiary separator and a stirring assembly; the primary separator comprises a primary separation cavity body, wherein the primary separation cavity body is of a cylindrical structure with a bottom and a cover, a first annular shoulder is fixedly arranged on the outer wall of the upper end of the primary separation cavity body, the primary separation cavity body penetrates through the through hole and is clamped on the fixed plate through the first annular shoulder, a hollow first installation boss is arranged in the middle of the inner bottom surface of the primary separation cavity body, a first discharge hole is uniformly formed in the outer ring of the bottom of the first installation boss around the central axis of the first installation boss, and first cutting teeth are uniformly arranged on the inner side wall of the primary separation cavity body; the secondary separator comprises a secondary separation cavity body and a first toothed ring, wherein the secondary separation cavity body is of a cylindrical structure with a bottom and without a cover, a second annular shoulder is fixedly arranged on the outer wall of the upper end of the secondary separation cavity body, the first toothed ring is fixedly sleeved on the outer wall of the second annular shoulder, a first stepped hole with a small bottom and a large top is formed in the bottom of the secondary separation cavity body corresponding to the first mounting boss, the lower part of the first stepped hole is clamped on the first mounting boss, the first mounting boss is rotationally connected with the secondary separation cavity body, a hollow second mounting boss is arranged on the inner bottom surface of the secondary separation cavity body corresponding to the first stepped hole, a second discharging hole is uniformly formed in the outer ring of the bottom of the second mounting boss, a second cutting tooth is uniformly arranged on the inner side wall of the secondary separation cavity body, a plurality of longitudinal first stirring ribs are uniformly arranged on the outer side wall of the secondary separation cavity body, and a plurality of first filtering holes, the second cutting teeth and the first stirring ribs are formed in the side wall of the secondary separation cavity body, and the first filtering holes are not interfered with the second cutting teeth and the first stirring ribs are uniformly arranged on the side wall; the three-stage separator comprises a three-stage separation cavity body and a second toothed ring, wherein the three-stage separation cavity body is of a cylindrical structure with a bottom and a cover, a third annular shoulder is fixedly arranged on the outer wall of the upper end of the three-stage separation cavity body, the second toothed ring is fixedly sleeved on the outer wall of the third annular shoulder, a second stepped hole with a small upper part and a large lower part is formed in the bottom of the three-stage separation cavity body corresponding to the second installation boss, the Kong Xia part of the second stepped hole is clamped on the second installation boss, the second installation boss is rotationally connected with the three-stage separation cavity body, a hollow third installation boss is formed in the inner bottom surface of the three-stage separation cavity body corresponding to the second stepped hole, a third discharge hole is uniformly formed in the outer ring of the bottom of the third installation boss, a third cutting tooth is uniformly formed in the inner side wall of the three-stage separation cavity body, a plurality of longitudinal second stirring ribs are uniformly formed in the outer side wall of the three-stage separation cavity body, a plurality of second filtering holes are formed in the side wall of the three-stage separation cavity body, the second filtering holes do not interfere with the third cutting tooth and the second stirring ribs, and the top of the third installation boss is fixedly connected with a pressing block, and the pressing block is correspondingly arranged in the middle part of the step hole and the cavity is communicated with the cavity; the stirring assembly comprises a stirring rod, stirring blades and a third toothed ring, wherein the upper end of the stirring rod penetrates through the mounting support and is rotationally connected with the mounting support, a rotating groove corresponding to a pressing block is formed in the middle of the lower end of the stirring rod, the pressing block is clamped into the rotating groove, the outer wall of the top end of the pressing block is rotationally connected with the inner wall of the rotating groove, a feeding cavity is formed in the middle of the stirring rod and is positioned on the upper side of the rotating groove, feeding holes are uniformly formed in the outer wall of the stirring rod at the bottom of the feeding cavity and around the central axis of the stirring rod, the stirring blades are uniformly arranged on the outer wall of the stirring rod positioned in the three-stage separation cavity body and do not interfere with the feeding holes, and the third toothed ring is fixedly sleeved on the outer wall of the stirring rod positioned on the upper side of the three-stage separation cavity body;
the sliding component comprises a sliding rod, a screw rod, a connecting rod, a discharging rod and a first motor; a sliding rod is arranged on the fixed plate at one side of the separation assembly far away from the positioning bracket, and penetrates through the fixed plate and is in sliding connection with the fixed plate; a connecting rod is fixedly sleeved on the outer wall of the bottom of the sliding rod, and one end of the connecting rod, which is far away from the sliding rod, is fixedly connected with a discharging rod with a hollow inside; the central axis of the discharging rod is parallel to the central axis of the sliding rod, the discharging rod penetrates through the connecting rod, the upper end of the discharging rod sequentially penetrates through the bottom surface of the primary separation cavity body, the first installation boss, the first stepped hole, the second installation boss, the second stepped hole and the third installation boss from bottom to top and is positioned in the cavity, the end part of the upper end of the discharging rod is provided with a material receiving hole around the axis of the discharging rod, the material receiving hole corresponds to the third discharging hole, the second discharging hole and the first discharging hole, and the initial position of the material receiving hole is positioned on the upper side of the third discharging hole; the screw rod penetrates through the bottom of the sliding rod and is in threaded connection with the sliding rod, and one end, away from the sliding rod, of the screw rod is fixedly connected with a first motor output shaft fixedly arranged on the base;
the driving assembly comprises a second motor, a rotating rod, a first gear, a second gear and a third gear; the second motor is fixedly arranged on the upper end face of the mounting bracket corresponding to the sliding rod, the output end of the second motor penetrates through the mounting bracket and is positioned on the outer wall of the lower side of the mounting bracket to be sleeved with the rotating rod, the outer wall of the output end of the second motor is in sliding connection with the inner wall of the rotating rod, the outer wall of the rotating rod is fixedly sleeved with a third gear, a second gear and a first gear from top to bottom in sequence, the third gear can be meshed with a third toothed ring, the second gear can be meshed with a second toothed ring, and the first gear can be meshed with the first toothed ring; the lower end of the rotating rod is embedded into the upper end of the sliding rod and is connected in a rotating way.
And further optimizing, the base and the positioning support and the mounting support are fixedly connected through screws or bolts.
And the fixing plate is further optimized, so that the fixing plate is completely fixed, and the fixing plate is prevented from shaking in the process of cutting tumor tissues, and one end of the fixing plate, which is far away from the positioning support, is fixedly connected with the upper end face of the base through two arc-shaped positioning columns.
And further optimizing, wherein in order to ensure that the primary separation cavity body and the fixing plate do not rotate relatively, positioning pins are uniformly distributed at the bottom of the first annular shoulder and around the central axis of the first annular shoulder, positioning holes are formed in the top surface of the fixing plate corresponding to the positioning pins, and the positioning pins are clamped into the positioning holes.
And in order to avoid liquid leakage, a sealing rubber ring is arranged at the joint of the discharging rod and the bottom surface of the primary separation cavity body, the joint of the first installation boss and the first stepped hole, the joint of the second installation boss and the second stepped hole and the joint of the third installation boss and the pressing block.
Further preferably, the second filter aperture diameter is greater than the first filter aperture diameter.
The method is further optimized, a sealing bearing is arranged between the inner wall of the top end of the primary separation cavity body and the outer wall of the secondary separation cavity body, which is positioned at the lower side of the second annular shoulder, between the inner wall of the top end of the secondary separation cavity body and the outer wall of the tertiary separation cavity body, which is positioned at the lower side of the third annular shoulder, between the inner wall of the tertiary separation cavity body and the outer wall of the stirring rod, which is positioned at the upper side of the stirring blade and at the lower side of the third toothed ring, and between the outer wall of the top end of the pressing block and the inner wall of the rotating groove, and the rotation among the secondary separation cavity body, the tertiary separation cavity body and the stirring rod is not affected by each other through the arrangement of the sealing bearing; secondly, the influence of the external environment caused by the splashing of the tissues during the tissue cutting is avoided, and meanwhile, the inaccuracy of the subsequent detection result caused by the pollution of the external environment to the tissues during the tissue cutting is also avoided.
And further optimizing, the stirring rod is rotationally connected with the mounting bracket through a ball bearing.
And further optimizing, the size of the first discharging hole is smaller than that of the second discharging hole and smaller than that of the third discharging hole, the size of the receiving hole is not smaller than that of the third discharging hole, and the size of the feeding hole is not smaller than three times that of the receiving hole.
And further optimizing, the diameter of the first gear ring is larger than that of the second gear ring and larger than that of the third gear ring, and the diameter of the corresponding first gear is smaller than that of the second gear and smaller than that of the third gear.
Further preferably, the thickness of the first gear is smaller than that of the second gear and smaller than that of the third gear.
Preferably, the number of the first stirring ribs, the second stirring ribs and the stirring blades is 5-10.
And the top of the feeding cavity is communicated with a feeding pipe for adding tumor tissues.
Further optimized, the upper end surface of the base is fixedly provided with a liquid discharge pipe corresponding to the discharge rod, the lower end of the discharge rod extends into the liquid discharge pipe, and the outer wall of the discharge rod is in sliding connection with the inner wall of the liquid discharge pipe; one side of the lower end of the liquid discharge pipe is communicated with a collecting pipe, and an electromagnetic valve is arranged on the collecting pipe.
And the sliding rod is positioned on the outer wall of the upper side of the connecting rod, sliding strips are uniformly arranged on the outer wall of the upper side of the connecting rod, the fixing plate is provided with vertical sliding grooves corresponding to the sliding strips, the sliding strips are clamped into the sliding grooves and are in sliding connection, and the sliding rods do not rotate relatively and only slide up and down in the screw rotating process.
And the second motor output shaft is positioned on the outer wall of the lower side of the mounting bracket and is provided with a spline, the upper end of the rotating rod is provided with a spline hole corresponding to the spline, the spline is clamped into the spline hole and is in sliding connection, so that the rotating rod can follow the rotation when the second motor rotates, and meanwhile the sliding rod can slide along the sliding rod when the sliding rod slides.
And the lower end of the rotating rod is rotationally connected with the upper end of the sliding rod through a rotating bearing, and the rotating bearing is clamped into the upper end of the sliding rod and is in interference fit with the rotating shaft bearing, so that the rotating rod can slide along with the sliding rod when the sliding rod slides, and the rotation of the rotating rod is not influenced by the sliding rod.
The invention has the following technical effects:
according to the tumor tissue collecting device, multistage separation of tumor organs and tissues is realized through cooperation of the primary separator, the secondary separator, the tertiary separator and the stirring assembly, and the tumor tissues with different sizes can be effectively collected through cooperation of the sliding assembly and the driving assembly, so that the collecting range is wide, and the collecting efficiency is high; meanwhile, the equipment does not need to replace equipment and a cutter, and tumor tissues in the cutting process can not be contacted with the external environment, so that the problems that tissues are polluted in the collecting process and the detection result is inaccurate are effectively avoided. Through the cooperation of ring gear and gear (first ring gear corresponds first ring gear, second ring gear corresponds the second ring gear, third ring gear corresponds the third ring gear promptly), realize the speed adjustment on the single actuating device, ensure that initial cutting process tumour tissue is smashed effectively, improve collection efficiency (i.e. big rate cutting), avoid the cell to be destroyed in the little tissue cutting process simultaneously, effectively remain the tissue activity (i.e. little rate cutting), thereby realize the effective collection and the inspection of multistage size tissue, ensure that the scope of inspection is wide, the accuracy is high, guarantee the integrality and the accuracy of testing result. Through the cooperation of first motor, lead screw, sliding rod, dwang and second motor, realize that second motor drive gear is to (first gear, second gear and third gear promptly) not receive the influence of first motor when rotating, guarantee simultaneously again that first motor can drive the gear pair and remove, guarantee the smooth operation of whole equipment.
The method can finish operations of hierarchical cutting, enzymolysis, hierarchical collection and filtration of tumor organ tissues in the same device, effectively fuses different functions, does not need to replace equipment or transfer tissues, effectively avoids tissue pollution, improves cutting and separating efficiency, saves separating time, and therefore breaks seconds for analysis and treatment of clinical medicine and promotes development of clinical medicine.
Drawings
Fig. 1 is a schematic diagram of the overall structure of a multi-stage processing apparatus according to an embodiment of the present invention.
Fig. 2 is a cross-sectional view of a multi-stage processing apparatus in an embodiment of the invention.
Fig. 3 is an enlarged view of a portion of fig. 2.
Fig. 4 is a B-B cross-sectional view of fig. 2.
Fig. 5 is a cross-sectional view taken along the direction C-C of fig. 2.
FIG. 6 is a schematic diagram of a primary separator of a multi-stage treatment apparatus according to an embodiment of the present invention; fig. 6 (a) is a perspective view of the primary separator, and fig. 6 (b) is a sectional view of the primary separator.
FIG. 7 is a schematic diagram of a secondary separator of a multi-stage treatment apparatus according to an embodiment of the present invention; fig. 7 (a) is a perspective view of the secondary separator, and fig. 7 (b) is a sectional view of the secondary separator.
FIG. 8 is a schematic diagram of a three stage separator of a multi-stage treatment apparatus in accordance with an embodiment of the present invention; fig. 8 (a) is a perspective view of the three-stage separator, and fig. 8 (b) is a sectional view of the three-stage separator.
Fig. 9 is a schematic structural view of a stirring assembly of a multistage processing apparatus in an embodiment of the present invention.
10, a base; 11. a liquid discharge pipe; 12. a collection pipe; 120. an electromagnetic valve; 13. arc-shaped positioning columns; 20. a positioning bracket; 30. a mounting bracket; 40. a fixing plate; 401. positioning holes; 402. a sliding groove; 50. a separation assembly; 501. sealing the rubber ring; 502. sealing the bearing; 51. a primary separator; 510. a primary separation chamber body; 511. a first annular shoulder; 5110. a positioning pin; 512. a first mounting boss; 513. a first discharge hole; 514. a first cutting tooth; 52. a secondary separator; 520. a secondary separation chamber body; 521. a second annular shoulder; 522. a first toothed ring; 523. a first stepped hole; 524. a second mounting boss; 525. a second discharge hole; 526. a second cutting tooth; 527. a first stirring rib; 528. a first filter aperture; 53. a third stage separator; 530. a three-stage separation chamber body; 531. a third annular shoulder; 532. a second toothed ring; 533. a second stepped hole; 534. a third mounting boss; 535. a third discharge hole; 536. a third cutting tooth; 537. a second stirring rib; 538. a second filter aperture; 54. a stirring assembly; 540. briquetting; 5401. a cavity; 541. a stirring rod; 5410. a ball bearing; 5411. a rotating groove; 5412. a feed chamber; 5413. a feed hole; 542. stirring blades; 543. a third toothed ring; 60. a slip assembly; 61. a sliding rod; 610. a rotating bearing; 611. a sliding bar; 62. a screw rod; 63. a connecting rod; 64. a discharging rod; 640. a receiving hole; 65. a first motor; 70. a drive assembly; 71. a second motor; 710. a spline; 72. a rotating lever; 720. a spline hole; 73. a third gear; 74. a second gear; 75. a first gear.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Examples:
as shown in fig. 1 to 9, a tumor organ separation treatment method is characterized in that: the device comprises a step-by-step separation and collection method, wherein the step-by-step separation and collection are performed by adopting multi-stage treatment equipment, and the multi-stage treatment equipment comprises a base 10, a positioning bracket 20, a mounting bracket 30, a fixed plate 40, a separation assembly 50, a sliding assembly 60 and a driving assembly 70; the bottom of the positioning bracket 20 is fixedly connected with one side of the upper end surface of the base 10, and one end of the positioning bracket 20, which is far away from the base 10, is fixedly connected with the mounting bracket 30; the middle part of the positioning bracket 20 is fixedly provided with a fixing plate 40, and the middle part of the fixing plate 40 is provided with a through hole;
the separation assembly 50 includes a primary separator 51, a secondary separator 52, a tertiary separator 53, and a stirring assembly 54; the primary separator 51 comprises a primary separation chamber body 510, the primary separation chamber body 510 is of a cylindrical structure with a bottom and without a cover, a first annular convex shoulder 511 is fixedly arranged on the outer wall of the upper end of the primary separation chamber body 510 (as shown in fig. 6), the primary separation chamber body 510 penetrates through a through hole (namely the middle part of the fixed plate 40) and is clamped on the fixed plate 40 through the first annular convex shoulder 511 (as shown in fig. 1 and 2), a hollow first installation boss 512 is arranged in the middle of the inner bottom surface of the primary separation chamber body 510, a first discharging hole 513 is uniformly formed around the central axis of the bottom outer ring of the first installation boss 512, and first cutting teeth 514 are uniformly arranged on the inner side wall of the primary separation chamber body 510; the second separator 52 comprises a second separation chamber body 520 and a first toothed ring 522, the second separation chamber body 520 is of a cylindrical structure with a bottom and without a cover, a second annular shoulder 521 is fixedly arranged on the outer wall of the upper end of the second separation chamber body 520 (as shown in fig. 7), the first toothed ring 522 is fixedly sleeved on the outer wall of the second annular shoulder 521, a first stepped hole 523 with a small bottom and a large top is arranged at the bottom of the second separation chamber body 520 corresponding to the first mounting boss 512, the lower part of the first stepped hole 523 is clamped on the first mounting boss 512 (as shown in fig. 2), the large diameter part of the lower part of the first stepped hole 523 is clamped on the upper side of the first mounting boss 512 and does not seal the first discharging hole 513, namely, the bottom surface of the second separation chamber body 520 is positioned on the upper side of the first discharging hole 513), the first mounting boss 512 is in rotary connection with the second separation chamber body 520, the inner bottom surface of the second separation chamber body 520 is provided with a hollow second mounting boss 524 corresponding to the first stepped hole 523, the second mounting boss bottom is uniformly arranged around the axis 524, the second discharging hole is uniformly arranged on the inner side wall of the second separation chamber body 520, the inner side wall of the second separation chamber body 520 is uniformly provided with second cutting teeth 526, and the second separation chamber body 527 is uniformly arranged on the outer side wall 527 is provided with a plurality of second stirring ribs 528, as shown in fig. 7, and the first stirring ribs 528 are uniformly arranged on the outer side wall of the second separation chamber body and a stirring side wall 528 is not provided; the three-stage separator 53 comprises a three-stage separating chamber body 530 and a second toothed ring 532, the three-stage separating chamber body 530 has a cylindrical structure with a bottom and no cover, the outer wall of the upper end of the three-stage separating chamber body is fixedly provided with a third annular shoulder 531 (as shown in figure 8), the second toothed ring 532 is fixedly sleeved on the outer wall of the third annular shoulder 531, the bottom of the three-stage separating chamber 530 body is provided with a second stepped hole 533 with a small bottom and a large top corresponding to the second mounting boss 524, the lower part of the second stepped hole 533 is clamped on the second mounting boss 524 (as shown in figure 2), the large diameter part of the lower part of the second stepped hole 533 is clamped on the upper side of the second mounting boss 524 and does not seal the second discharging hole 525, namely, the bottom surface of the three-stage separating chamber body 530 is positioned on the upper side of the second discharging hole 525), the second mounting boss 524 is rotationally connected with the three-stage separating chamber body 530, the inner bottom surface of the three-stage separation cavity body 530 is provided with a hollow third installation boss 534 corresponding to the second stepped hole 533, the outer ring at the bottom of the third installation boss 534 is uniformly provided with a third discharge hole 535 around the central axis, the inner side wall of the three-stage separation cavity body 530 is uniformly provided with a third cutting tooth 536, the outer side wall of the three-stage separation cavity body 530 is uniformly provided with a plurality of longitudinal second stirring ribs 537, the side wall of the three-stage separation cavity body 530 is provided with a plurality of second filtering holes 538, the second filtering holes 538 do not interfere with the third cutting tooth 536 and the second stirring ribs 537 (as shown in fig. 8), the top of the third installation boss 534 is fixedly connected with a pressing block 540, the middle part of the pressing block 540 is provided with a cavity 5401 corresponding to the second stepped hole 533, and the cavity 5401 is communicated with the second stepped hole 533 (as shown in fig. 2); the stirring assembly 54 includes a stirring rod 541, a stirring blade 542 and a third toothed ring 543, where the upper end of the stirring rod 541 penetrates through the mounting bracket 30 and is rotationally connected with the mounting bracket 30 through a ball bearing 5410, a rotating groove 5411 corresponding to the pressing block 540 is provided in the middle of the lower end of the stirring rod 541, the pressing block 540 is clamped into the rotating groove 5411, the outer wall of the top end of the pressing block 540 is rotationally connected with the inner wall of the rotating groove 5411 (as shown in fig. 2), a feeding cavity 5412 is provided in the middle of the stirring rod 541 and is located at the upper side of the rotating groove 5411 (the feeding cavity 5412 is not communicated with the rotating groove 5411), the outer wall of the stirring rod 541 at the bottom of the feeding cavity 5412 is uniformly provided with a feeding hole 5413 around the central axis of the stirring rod 541, the outer wall of the stirring rod 541 located in the three-stage separation cavity body 530 is uniformly provided with stirring blades 542, and the stirring blades 542 do not interfere with the feeding hole 5413 (i.e. the stirring blades 542 do not seal the feeding hole 5413), and the outer wall of the stirring rod 541 located at the upper side of the three-stage separation cavity body 530 is fixedly sleeved with the third toothed ring 543;
the sliding component 60 comprises a sliding rod 61, a screw 62, a connecting rod 63, a discharging rod 64 and a first motor 65; the fixed plate 40 on the side of the separating assembly 50 away from the positioning bracket 20 is provided with a sliding rod 61, and the sliding rod 61 penetrates through the fixed plate 40 and is in sliding connection with the fixed plate 40; a connecting rod 63 is fixedly sleeved on the outer wall of the bottom of the sliding rod 61, and one end of the connecting rod 63 away from the sliding rod is fixedly connected with a discharging rod 64 with a hollow inside; the central axis of the discharging rod 64 is parallel to the central axis of the sliding rod 61, the discharging rod 64 penetrates through the connecting rod 63 (as shown in fig. 9), the upper end of the discharging rod 64 sequentially penetrates through the bottom surface of the primary separation cavity body 510, the first installation boss 512, the first stepped hole 523, the second installation boss 524, the second stepped hole 533 and the third installation boss 534 from bottom to top and is positioned in the cavity 5401, the upper end part of the discharging rod 64 is provided with a receiving hole 640 around the axis thereof, the receiving hole 640 corresponds to the third discharging hole 535, the second discharging hole 525 and the first discharging hole 513, and the initial position of the receiving hole 640 is positioned on the upper side of the third discharging hole 535; the screw rod through 62 penetrates through the bottom of the sliding rod 61 and is in threaded connection with the sliding rod 61, and one end of the screw rod 62, which is far away from the sliding rod 61, is fixedly connected with an output shaft of a first motor 65 fixedly arranged on the base 10;
the driving assembly 70 includes a second motor 71, a rotating rod 72, a first gear 75, a second gear 74, and a third gear 73; the second motor 71 is fixedly arranged on the upper end surface of the mounting bracket 30 corresponding to the sliding rod 61, the output end of the second motor 71 penetrates through the mounting bracket 30 and is positioned on the outer wall of the lower side of the mounting bracket 30 to be sleeved with the rotating rod 72, the outer wall of the output end of the second motor 71 is slidably connected with the inner wall of the rotating rod 72, the outer wall of the rotating rod 72 is fixedly sleeved with a third gear 73, a second gear 74 and a first gear 75 from top to bottom in sequence, and the third gear 73 can be meshed with a third toothed ring 543, the second gear 74 and a second toothed ring 532 as well as the first gear 75 and the first toothed ring 522; the lower end of the rotating rod 72 is embedded into the upper end of the sliding rod 61 and is rotatably connected.
The base 10 and the positioning bracket 20, and the positioning bracket 20 and the mounting bracket 30 are fixedly connected by screws or bolts (as shown in fig. 2). To ensure that the fixing plate is completely fixed and to avoid shaking during the process of cutting tumor tissue, the end of the fixing plate 40, which is far away from the positioning bracket 20, is fixedly connected with the upper end surface of the base 10 through two arc-shaped positioning posts 13.
In order to ensure that the primary separation chamber body 510 and the fixing plate 40 do not rotate relatively, the positioning pins 5110 are uniformly distributed around the central axis of the first annular shoulder 511 at the bottom, and the top surface of the fixing plate 40 is provided with the positioning holes 401 corresponding to the positioning pins 5110, and the positioning pins 5110 are inserted into the positioning holes 401.
In order to avoid liquid leakage, a sealing rubber ring 501 is arranged at the junction of the discharging rod 64 and the bottom surface of the primary separation chamber body 510, the junction of the first mounting boss 512 and the first stepped hole 523, the junction of the second mounting boss 524 and the second stepped hole 533, and the junction of the third mounting boss 534 and the pressing block 540. The second filter aperture 538 has a diameter that is greater than the first filter aperture 528. A seal bearing 502 (as shown in fig. 2) is arranged between the inner wall of the top end of the primary separation chamber body 510 and the outer wall of the secondary separation chamber body 520 positioned at the lower side of the second annular shoulder 521, between the inner wall of the top end of the secondary separation chamber body 520 and the outer wall of the tertiary separation chamber body 530 positioned at the lower side of the third annular shoulder 531, between the inner wall of the tertiary separation chamber body 530 and the outer wall of the stirring rod 541 positioned at the upper side of the stirring vane 542 and positioned at the lower side of the third toothed ring 543, and between the outer wall of the top end of the pressing block 540 and the inner wall of the rotating groove 5411, and the rotation among the secondary separation chamber body 520, the tertiary separation chamber body 530 and the stirring rod 541 is not affected by each other by the arrangement of the seal bearing 502; secondly, the influence of the external environment caused by the splashing of the tissues during the tissue cutting is avoided, and meanwhile, the inaccuracy of the subsequent detection result caused by the pollution of the external environment to the tissues during the tissue cutting is also avoided. The first discharge hole 513 has a size smaller than the second discharge hole 525 and smaller than the third discharge hole 535 and the receiving hole 640 has a size not smaller than the third discharge hole 535, and the feeding hole 5413 has a size not smaller than three times the size of the receiving hole 640. The diameter of the first gear ring 522 is larger than that of the second gear ring 532 and the diameter of the third gear ring 543, and the diameter of the corresponding first gear 75 is smaller than that of the second gear 74 and smaller than that of the third gear 73; the thickness of the first gear 75 is smaller than the thickness of the second gear 74 and smaller than the thickness of the third gear 73. The number of the first stirring rib 527, the second stirring rib 537 and the stirring blade 542 is 5 to 10, preferably 8.
The top of the feed cavity 5412 communicates with a feed tube for adding tumor tissue. The upper end surface of the base 10 is fixedly provided with a liquid discharge pipe 11 corresponding to the discharge rod 64, the lower end of the discharge rod 64 extends into the liquid discharge pipe 11, and the outer wall of the discharge rod 64 is in sliding connection with the inner wall of the liquid discharge pipe 11; one side of the lower end of the liquid discharge pipe 11 is communicated with a collecting pipe 12, and an electromagnetic valve 120 is arranged on the collecting pipe 12.
The outer wall of the sliding rod 61 on the upper side of the connecting rod 63 is uniformly provided with sliding strips 611, the fixed plate 40 is provided with vertical sliding grooves 402 (as shown in fig. 4) corresponding to the sliding strips 611, the sliding strips 611 are clamped into the sliding grooves 402 and are in sliding connection, and therefore the sliding rod 61 does not rotate relatively and only slides up and down in the rotating process of the screw rod 62, and breakage between the sliding rod 61 and the connecting rod 63 due to torsional force of the screw rod 62 is avoided. The outer wall that the output shaft of second motor 71 is located the installing support 30 downside sets up spline 710, and the dwang 72 upper end corresponds spline 710 and sets up splined hole 720, and spline 710 card is gone into splined hole 720 and sliding connection, ensures that during the rotation of second motor 71, dwang 72 can follow the rotation, and simultaneously when sliding rod 61 slides, dwang 72 can follow sliding rod 61 and slide. The lower extreme of dwang 72 rotates with the slide bar 61 upper end through rolling bearing 610 and is connected, and rolling bearing 610 card goes into inside slide bar 61 upper end and dwang 72 and pivot bearing 610 interference fit to guarantee when slide bar 61 slides, dwang 72 can follow together and slide and the rotation of dwang 72 is not influenced by slide bar 61.
The step-by-step separation and collection method specifically comprises the following steps: when the device is used, the first motor 65 is started at first, the output shaft of the first motor 65 is rotated to drive the screw rod 62 to rotate, and the screw rod 62 is in threaded connection with the sliding rod 61, and the sliding rod 61 is in hard limit between the discharging rod 64 and the sliding bar 611 and the sliding groove 402 through the connecting rod 63, so that the sliding rod 61 can only slide up and down when the screw rod 62 rotates, the sliding rod 61 moves down to drive the discharging rod 64 through the connecting rod 63, so that the material receiving hole 640 is overlapped with the third discharging hole 535, meanwhile, the sliding rod 61 moves down to drive the first gear 75, the second gear 74 and the third gear 73 through the rotating rod 72, the third gear 73 is meshed with the third toothed ring 543 and the second gear 74 is not meshed with the second toothed ring 532, the first gear 75 is not meshed with the first toothed ring 522, the first motor 65 is stopped, the second motor 71 is started slowly, the second motor 71 drives the rotating rod 72 through the spline 710 to rotate, so that the first gear 75, the second gear 74 and the third gear 541 are driven by the third toothed ring 541 to rotate, and the third gear 73 drives the stirring rod through the third toothed ring 543. Then, the tumor tissue and the digestive juice after preliminary treatment are added into the feeding cavity 5412 through the feeding pipe, then the second motor 71 is accelerated, the tumor tissue and the digestive juice after preliminary treatment are thrown out through the feeding hole 5413, then the tumor tissue and the digestive juice are cut and crushed through the interaction force between the stirring blade 542 and the third cutting tooth 536, a part of the crushed tumor tissue flows into the secondary separation cavity body 520 through the centrifugal force and the second filtering hole 538, and a part of the crushed tumor tissue enters the liquid discharge pipe 11 through the third discharging hole 535, the material collecting hole 640 and the discharging rod 64, and at the moment, the electromagnetic valve 120 is opened to collect the tumor tissue through the collecting pipe 12.
Then the second motor 71 is turned off, the first motor 65 is started, the sliding rod 61 moves downwards and drives the discharging rod 64 and the rotating rod 72 to move downwards, the discharging rod 64 enables the collecting hole 640 to coincide with the second discharging hole 525, the rotating rod 72 moves downwards, the third gear 73 is meshed with the third toothed ring 543, the second gear 74 is meshed with the second toothed ring 532, the first gear 75 is not meshed with the first toothed ring 522, then the first motor 65 is turned off, the second motor 71 is started, the rotating rod 72 is rotated, the stirring rod 541 and the third-stage separation cavity body 530 are driven to rotate in the same direction, the interaction force of the second stirring ribs 537 and the second cutting teeth 526 cut and crush tumor tissues in the second-stage separation cavity body 520, a part of crushed tumor tissues flows into the first-stage separation cavity body 510 through centrifugal force and the first filtering hole 528, and a part of the crushed tumor tissues enters the liquid discharge pipe 11 through the second discharging hole 525, the collecting hole 640 and the discharging rod 64, and then the electromagnetic valve 120 is turned on, and the tumor tissues are collected through the collecting pipe 12. Meanwhile, since the diameter of the third gear 73 is larger than that of the second gear 74 and the diameter of the third gear ring 543 is smaller than that of the second gear ring 532, the rotation speeds of the stirring rod 541 and the third separation chamber body 530 are different (the rotation speed of the stirring rod 541 is larger than that of the third separation chamber body 530), so that the relative acting force of the stirring blade 542 and the third cutting tooth 536 is formed, the tumor tissue in the third separation chamber body 530 is further cut, the tumor tissue is guaranteed to flow into the second separation chamber body 520, and full cutting is realized.
Then the second motor 71 is turned off, the first motor 65 is started, the sliding rod 61 moves downwards and drives the discharging rod 64 and the rotating rod 72 to move downwards, the discharging rod 64 enables the collecting hole 640 to coincide with the first discharging hole 513, the rotating rod 72 moves downwards, the third gear 73 is meshed with the third toothed ring 543, the second gear 74 is meshed with the second toothed ring 532, the first gear 75 is meshed with the first toothed ring 522, then the first motor 65 is turned off, the second motor 71 is started, the rotating rod 72 is enabled to rotate, the stirring rod 541, the third-stage separation cavity body 530 and the second-stage separation cavity body 520 are driven to rotate in the same direction, the interaction force of the first stirring rib 527 and the first cutting tooth 514 cuts and pulverizes tumor tissue in the first-stage separation cavity body 510, the pulverized tumor tissue enters the liquid discharge pipe 11 through the first discharging hole 513, the collecting hole 640 and the discharging rod 64, and at the moment, the electromagnetic valve 120 is opened, and the tumor tissue is collected through the collecting pipe 12. Meanwhile, since the third gear 73 has a larger diameter than the second gear 74 and larger diameter than the first gear 75, and the third gear ring 543 has a smaller diameter than the second gear ring 532 and smaller diameter than the first gear ring 522, the rotation rates of the stirring rod 541, the third-stage separation chamber body 530 and the second-stage separation chamber body 520 are different (the rotation rate of the stirring rod 541 is larger than that of the third-stage separation chamber body 530 and larger than that of the second-stage separation chamber body 520), so that the relative acting force of the stirring blade 542 and the third cutting tooth 536 and the acting force between the second stirring rib 537 and the second cutting tooth 526 are formed, and the tumor tissues in the third-stage separation chamber body 530 and the second-stage separation chamber body 520 are further cut, thereby realizing full cutting.
The process can realize the cutting and collecting of tumor tissues with different sizes without equipment or cutter replacement, and can obtain larger cell clusters, small cell clusters, free cells and the like in stages, so that the detection range is wide and the accuracy is high; meanwhile, the cutting rate is gradually reduced due to the relation of the gear pair, so that the damage to the tumor tissue of the small cell mass due to the cutting at a high rotating speed is avoided, and the test result is influenced.
After the tumor tissue is separated, the cleaning liquid is introduced from the feeding pipe, and the second motor 71 is adjusted to run at a high speed, so that the inside of the whole device can be cleaned uniformly, the first motor 65 can be controlled to select, the cleaning liquid is completely discharged out of the device, and the cleaning is convenient and the targeted cleaning can be performed.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. A tumor organ separation treatment method is characterized in that: the device comprises a step-by-step separation and collection method, wherein the step-by-step separation and collection are performed by adopting multi-stage treatment equipment, and the multi-stage treatment equipment comprises a base (10), a positioning bracket (20), a mounting bracket (30), a fixing plate (40), a separation assembly (50), a sliding assembly (60) and a driving assembly (70); the bottom of the positioning bracket (20) is fixedly connected with one side of the upper end face of the base (10), and one end, far away from the base (10), of the positioning bracket (20) is fixedly connected with the mounting bracket (30); a fixed plate (40) is fixedly arranged in the middle of the positioning bracket (20), and a through hole is formed in the middle of the fixed plate (40);
the separation assembly (50) comprises a primary separator (51), a secondary separator (52), a tertiary separator (53) and a stirring assembly (54); the primary separator (51) comprises a primary separation cavity body (510), the primary separation cavity body (510) is of a cylindrical structure with a bottom and without a cover, a first annular shoulder (511) is fixedly arranged on the outer wall of the upper end of the primary separation cavity body, the primary separation cavity body (510) penetrates through the through hole and is clamped on the fixed plate (40) through the first annular shoulder (511), a hollow first installation boss (512) is arranged in the middle of the inner bottom surface of the primary separation cavity body (510), a first discharge hole (513) is uniformly formed in the middle of the outer ring of the bottom of the first installation boss (512), and first cutting teeth (514) are uniformly arranged on the inner side wall of the primary separation cavity body (510); the secondary separator (52) comprises a secondary separation cavity body (520) and a first gear ring (522), the secondary separation cavity body (520) is of a cylindrical structure with a bottom and without a cover, a second annular convex shoulder (521) is fixedly arranged on the outer wall of the upper end of the secondary separation cavity body, the first gear ring (522) is fixedly sleeved on the outer wall of the second annular convex shoulder (521), a first stepped hole (523) with a small upper part and a large lower part is formed at the bottom of the secondary separation cavity body (520) corresponding to the first installation boss (512), the lower part of the first stepped hole (523) is clamped into the first installation boss (512), the first installation boss (512) is rotationally connected with the secondary separation cavity body (520), the inner bottom surface of the secondary separation cavity body (520) is provided with a hollow second installation boss (524) corresponding to the first stepped hole (523), a second discharge hole (525) is uniformly formed around the central axis of the second installation boss (524), the inner side wall of the secondary separation cavity body (520) is uniformly provided with a second cutting tooth (526), the outer side wall of the secondary separation cavity body (520) is uniformly provided with a plurality of longitudinal first stirring ribs (527), the side wall of the secondary separation cavity body (520) is provided with a plurality of first filtering holes (528), and the first filtering holes (528) do not interfere with the second cutting tooth (526) and the first stirring ribs (527); the third-stage separator (53) comprises a third-stage separation cavity body (530) and a second toothed ring (532), the third-stage separation cavity body (530) is of a cylindrical structure with a bottom and without a cover, a third annular shoulder (531) is fixedly arranged on the outer wall of the upper end of the third-stage separation cavity body, the second toothed ring (532) is fixedly sleeved on the outer wall of the third annular shoulder (531), a second stepped hole (533) with a small upper part and a large lower part is formed at the bottom of the third-stage separation cavity body (530) corresponding to the second mounting boss (524), the lower part of the second stepped hole (533) is clamped on the second mounting boss (524), the second mounting boss (524) is in rotary connection with the third-stage separation cavity body (530), a hollow third mounting boss (534) is arranged at the inner bottom of the third-stage separation cavity body corresponding to the second stepped hole (533), a third discharging hole (535) is uniformly formed at the outer ring of the bottom of the third mounting boss (534), a third cutting tooth (536) is uniformly formed on the inner side wall of the third-stage separation cavity body (530) and a plurality of second-stage separation cavity (538) and a plurality of second-stage separation rib (538) are uniformly arranged, the second-stage separation rib (538) is uniformly arranged on the side wall (538) and is uniformly interfered with the second stirring rib (537), a pressing block (540) is fixedly connected to the top of the third installation boss (534), a cavity (5401) is formed in the middle of the pressing block (540) corresponding to the second stepped hole (533), and the cavity (5401) is communicated with the second stepped hole (533); the stirring assembly (54) comprises a stirring rod (541), stirring blades (542) and a third toothed ring (543), wherein the upper end of the stirring rod (541) penetrates through the mounting bracket (30) and is in rotary connection with the mounting bracket (30), a rotary groove (5411) corresponding to a pressing block (540) is formed in the middle of the lower end of the stirring rod (541), the pressing block (540) is clamped into the rotary groove (5411), the outer wall of the top end of the pressing block (540) is in rotary connection with the inner wall of the rotary groove (5411), a feeding cavity (5412) is formed in the middle of the stirring rod (541) and is positioned on the upper side of the rotary groove (5411), feeding holes (5413) are uniformly formed in the outer wall of the stirring rod (541) at the bottom of the feeding cavity (5412) and around the central axis of the stirring rod (541), the stirring blades (542) are uniformly arranged on the outer wall of the stirring rod (541) in the three-stage separation cavity body (530), the stirring rod (541) is not interfered with the feeding holes (5413), and the third toothed ring (543) is fixedly arranged on the outer wall of the stirring rod (541) at the upper side of the three-stage separation cavity body (530);
the sliding assembly (60) comprises a sliding rod (61), a screw rod (62), a connecting rod (63), a discharging rod (64) and a first motor (65); a sliding rod (61) is arranged on the fixed plate (40) at one side of the separation assembly (50) far away from the positioning bracket (20), and the sliding rod (61) penetrates through the fixed plate (40) and is connected with the fixed plate in a sliding manner; a connecting rod (63) is fixedly sleeved on the outer wall of the bottom of the sliding rod (61), and one end of the connecting rod (63) away from the sliding rod (61) is fixedly connected with a discharging rod (64) with a hollow inside; the central axis of the discharging rod (64) is parallel to the central axis of the sliding rod (61), the discharging rod (64) penetrates through the connecting rod (63), the upper end of the discharging rod (64) sequentially penetrates through the bottom surface of the primary separation cavity body (510), the first installation boss (512), the first stepped hole (523), the second installation boss (524), the second stepped hole (533) and the third installation boss (534) from bottom to top and is positioned in the cavity (5401), the material collecting hole (640) is formed at the end part of the upper end of the discharging rod (64) around the axis of the discharging rod, the material collecting hole (640) corresponds to the third discharging hole (535), the second discharging hole (525) and the first discharging hole (513), and the initial position of the material collecting hole (640) is positioned at the upper side of the third discharging hole (535); the screw rod (62) penetrates through the bottom of the sliding rod (61) and is in threaded connection with the sliding rod (61), and one end, far away from the sliding rod (61), of the screw rod (62) is fixedly connected with an output shaft of a first motor (65) fixedly arranged on the base (10);
the driving assembly (70) comprises a second motor (71), a rotating rod (72), a first gear (75), a second gear (74) and a third gear (73); the second motor (71) is fixedly arranged on the upper end face of the mounting bracket (30) corresponding to the sliding rod (61), the output end of the second motor (71) penetrates through the mounting bracket (30) and is positioned on the outer wall of the lower side of the mounting bracket (30) to be sleeved with the rotating rod (72), the outer wall of the output end of the second motor (71) is slidably connected with the inner wall of the rotating rod (72), the outer wall of the rotating rod (72) is fixedly sleeved with a third gear (73), a second gear (74) and a first gear (75) from top to bottom in sequence, and the third gear (73) can be meshed with a third toothed ring (543), the second gear (74) and a second toothed ring (532), and the first gear (75) and the first toothed ring (522); the lower end of the rotating rod (72) is embedded into the upper end of the sliding rod (61) and is connected in a rotating way.
2. The method for separating and treating a tumor organ according to claim 1, wherein: the base (10) is fixedly connected with the positioning support (20) and the positioning support (20) is fixedly connected with the mounting support (30) through screws or bolts.
3. A method for separating and treating a tumor organ according to claim 1 or 2, wherein: one end of the fixing plate (40) far away from the positioning bracket (20) is fixedly connected with the upper end face of the base (10) through two arc-shaped positioning columns (13).
4. A method for separating and treating a tumor organ according to claim 3, wherein: the discharging rod (64) is connected with the bottom surface of the primary separation cavity body (510), the first installation boss (512) is connected with the first stepped hole (523), the second installation boss (524) is connected with the second stepped hole (533), and the third installation boss (534) is connected with the pressing block (540) to form a sealing rubber ring (501).
5. A method for separating and treating a tumor organ according to claim 3, wherein: the sealing device is characterized in that a sealing bearing (502) is arranged between the inner wall of the top end of the primary separation cavity body (510) and the outer wall of the lower side of the second annular shoulder (521), between the inner wall of the top end of the secondary separation cavity body (520) and the outer wall of the lower side of the third annular shoulder (531), between the inner wall of the tertiary separation cavity body (530) and the stirring rod (541) and between the upper side of the stirring blade (542) and the outer wall of the lower side of the third toothed ring (543), and between the outer wall of the top end of the pressing block (540) and the inner wall of the rotating groove (5411).
6. The method for separating and treating a tumor organ according to claim 1, wherein: the stirring rod (541) is rotatably connected with the mounting bracket (30) through a ball bearing (5410).
7. The method for separating and treating a tumor organ according to claim 1, wherein: the diameter of the first toothed ring (522) is larger than that of the second toothed ring (532) and larger than that of the third toothed ring (543), and the diameter of the corresponding first gear (75) is smaller than that of the second gear (74) and smaller than that of the third gear (73).
8. The method for separating and treating a tumor organ according to claim 1, wherein: the top of the feeding cavity (5412) is communicated with a feeding pipe.
9. The method for separating and treating a tumor organ according to claim 1, wherein: the upper end face of the base (10) is fixedly provided with a liquid discharge pipe (11) corresponding to the discharging rod (64), the lower end of the discharging rod (64) extends into the liquid discharge pipe (11), and the outer wall of the discharging rod (64) is in sliding connection with the inner wall of the liquid discharge pipe (11); one side of the lower end of the liquid discharge pipe (11) is communicated with a collecting pipe (12), and an electromagnetic valve (120) is arranged on the collecting pipe (12).
10. The method for separating and treating a tumor organ according to claim 1, wherein: the lower end of the rotating rod (72) is rotationally connected with the upper end of the sliding rod (61) through a rotating bearing (610), the rotating bearing (610) is clamped into the inner part of the upper end of the sliding rod (61), and the rotating rod (72) is in interference fit with the rotating bearing (610).
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