CN114423870A - 核糖核酸的无细胞产生 - Google Patents

核糖核酸的无细胞产生 Download PDF

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CN114423870A
CN114423870A CN202080036375.7A CN202080036375A CN114423870A CN 114423870 A CN114423870 A CN 114423870A CN 202080036375 A CN202080036375 A CN 202080036375A CN 114423870 A CN114423870 A CN 114423870A
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rna
reaction mixture
kinase
mrna
cell
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Chinese (zh)
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安德雷·J·扎鲁尔
德鲁·S·坎宁安
詹姆斯·R·阿布希尔
拉基特·贾殷
迈克尔·E·哈德逊
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Greenlight Biosciences Inc
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Greenlight Biosciences Inc
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
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    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1229—Phosphotransferases with a phosphate group as acceptor (2.7.4)
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    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
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    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1258—Polyribonucleotide nucleotidyltransferase (2.7.7.8), i.e. polynucleotide phosphorylase
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/04—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C12Y207/04004—Nucleoside-phosphate kinase (2.7.4.4)
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/119—RNA polymerase
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    • C12Y207/04—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C12Y207/04001—Polyphosphate kinase (2.7.4.1)
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    • C12Y207/04—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C12Y207/04003—Adenylate kinase (2.7.4.3)
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    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/04—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C12Y207/04006—Nucleoside-diphosphate kinase (2.7.4.6)
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    • C12Y207/04—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C12Y207/04008—Guanylate kinase (2.7.4.8)
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    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/04—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C12Y207/04022—UMP kinase (2.7.4.22)
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    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/04—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C12Y207/04025—(d)CMP kinase (2.7.4.25)
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    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)
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    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07008—Polyribonucleotide nucleotidyltransferase (2.7.7.8), i.e. polynucleotide phosphorylase
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    • C12Y301/13—Exoribonucleases producing 5'-phosphomonoesters (3.1.13)
    • C12Y301/13001—Exoribonuclease II (3.1.13.1)
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    • C12Y301/30—Endoribonucleases active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters (3.1.30)
    • C12Y301/30001—Aspergillus nuclease S1 (3.1.30.1)

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CN202080036375.7A 2019-03-29 2020-03-30 核糖核酸的无细胞产生 Pending CN114423870A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962826983P 2019-03-29 2019-03-29
US62/826,983 2019-03-29
PCT/US2020/025824 WO2020205793A1 (en) 2019-03-29 2020-03-30 Cell-free production of ribonucleic acid

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CN114423870A true CN114423870A (zh) 2022-04-29

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US (1) US20220162659A1 (https=)
EP (1) EP3947704A4 (https=)
JP (1) JP2022524166A (https=)
KR (1) KR20220004057A (https=)
CN (1) CN114423870A (https=)
AU (1) AU2020253354A1 (https=)
CA (1) CA3135368A1 (https=)
IL (1) IL286633A (https=)
SG (1) SG11202110608YA (https=)
WO (1) WO2020205793A1 (https=)
ZA (1) ZA202107709B (https=)

Families Citing this family (10)

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KR20220113437A (ko) * 2019-12-06 2022-08-12 그린라이트 바이오사이언시스, 아이엔씨. 핵산 조성물
US12419948B2 (en) 2020-08-31 2025-09-23 The Broad Institute, Inc. Immunogenic compositions and use thereof
CN112521437A (zh) * 2020-12-03 2021-03-19 珠海丽凡达生物技术有限公司 mRNA体外转录过程中抗-反向帽类似物的回收方法、抗-反向帽类似物及应用
MX2023012833A (es) * 2021-04-30 2024-01-23 Greenlight Biosciences Inc Opciones terapéuticas y composiciones de arn mensajero.
CA3225545A1 (en) 2021-09-16 2023-03-23 Immunitybio, Inc. Enzyme based system for production of messenger rna with increased transfection efficiency
CN114736884B (zh) * 2022-05-11 2023-10-20 中国科学院合肥物质科学研究院 一种胞苷单磷酸激酶突变体及其基因和应用
US20240110234A1 (en) * 2022-09-30 2024-04-04 Illumina, Inc. Amplification Compositions and Methods
CN116463273B (zh) * 2023-03-14 2025-11-07 江苏香地化学有限公司 增强5’-胞苷酸积累的方法及应用
WO2025072383A1 (en) 2023-09-25 2025-04-03 The Broad Institute, Inc. Viral open reading frames, uses thereof, and methods of detecting the same
WO2025097055A2 (en) 2023-11-02 2025-05-08 The Broad Institute, Inc. Compositions and methods of use of t cells in immunotherapy

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US20060211083A1 (en) * 2005-01-21 2006-09-21 Federico Katzen Products and processes for in vitro synthesis of biomolecules
WO2013071047A1 (en) * 2011-11-11 2013-05-16 Children's Medical Center Corporation Compositions and methods for in vitro transcription of rna
CN109196109A (zh) * 2016-04-06 2019-01-11 绿光生物科技股份有限公司 无细胞生成核糖核酸

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060211083A1 (en) * 2005-01-21 2006-09-21 Federico Katzen Products and processes for in vitro synthesis of biomolecules
WO2013071047A1 (en) * 2011-11-11 2013-05-16 Children's Medical Center Corporation Compositions and methods for in vitro transcription of rna
CN109196109A (zh) * 2016-04-06 2019-01-11 绿光生物科技股份有限公司 无细胞生成核糖核酸

Non-Patent Citations (3)

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Title
AIMEE L. JALKANEN ET AL.: "Determinants and Implications of mRNA Poly(A) Tail Size - Does this Protein Make My Tail Look Big?", 《SEMIN CELL DEV BIOL.》 *
FABIAN MUTTACH ET AL.: "Synthetic mRNA capping", 《JOURNAL OF ORGANIC CHEMISTRY》 *
VICTORIA H. COWLING ET AL.: "Regulation of mRNA cap methylation", 《BIOCHEM. J.》 *

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Publication number Publication date
ZA202107709B (en) 2023-07-26
EP3947704A4 (en) 2022-12-21
JP2022524166A (ja) 2022-04-27
EP3947704A1 (en) 2022-02-09
CA3135368A1 (en) 2020-10-08
WO2020205793A1 (en) 2020-10-08
KR20220004057A (ko) 2022-01-11
US20220162659A1 (en) 2022-05-26
IL286633A (en) 2021-12-01
SG11202110608YA (en) 2021-10-28
AU2020253354A1 (en) 2021-10-28

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