CN114410466A - 用于个性化癌症治疗的精确药物筛选 - Google Patents

用于个性化癌症治疗的精确药物筛选 Download PDF

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Publication number
CN114410466A
CN114410466A CN202110357675.1A CN202110357675A CN114410466A CN 114410466 A CN114410466 A CN 114410466A CN 202110357675 A CN202110357675 A CN 202110357675A CN 114410466 A CN114410466 A CN 114410466A
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organ
spheres
patient
micro
cells
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Chinese (zh)
Inventor
沈西凌
丹尼尔·得鲁巴
大卫·徐
杰弗里·莫赫曼
王朝晖
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Hillis Corp
Duke University
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Individual
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Priority claimed from US16/838,010 external-priority patent/US11555180B2/en
Priority claimed from US17/178,210 external-priority patent/US20210285054A1/en
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    • C—CHEMISTRY; METALLURGY
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • G—PHYSICS
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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5076—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving cell organelles, e.g. Golgi complex, endoplasmic reticulum
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    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/575—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
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    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
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CN202110357675.1A 2020-04-01 2021-04-01 用于个性化癌症治疗的精确药物筛选 Pending CN114410466A (zh)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US16/838,010 US11555180B2 (en) 2019-05-28 2020-04-01 Methods and apparatuses for patient-derived micro-organospheres
US16/838,010 2020-04-01
US202063120719P 2020-12-02 2020-12-02
US63/120,719 2020-12-02
US202017118586A 2020-12-10 2020-12-10
US17/118,586 2020-12-10
US17/178,210 US20210285054A1 (en) 2019-05-28 2021-02-17 Precision drug screening for personalized cancer therapy
US17/178,210 2021-02-17

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Publication Number Publication Date
CN114410466A true CN114410466A (zh) 2022-04-29

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CN202120671205.8U Active CN218755788U (zh) 2020-04-01 2021-04-01 一种用于形成器官球的设备

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JP (1) JP2023554171A (https=)
KR (1) KR20230143134A (https=)
CN (2) CN114410466A (https=)
TW (1) TW202237858A (https=)
WO (1) WO2022119966A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114410466A (zh) * 2020-04-01 2022-04-29 沈西凌 用于个性化癌症治疗的精确药物筛选
US20260108889A1 (en) * 2022-10-12 2026-04-23 University Of Florida Research Foundation, Incorporated Collagen droplet-based 3d cell spheroids as 3d printable bio-ink

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WO2001079453A1 (fr) * 2000-04-18 2001-10-25 4C Biotech Procedes de culture de cellules, amas cellulaires obtenus par ces procedes et utilisation de ceux-ci
WO2007009036A2 (en) * 2005-07-12 2007-01-18 Tissue Genesis, Inc. Apparatus and methods for preparing tissue grafts
US20170355950A1 (en) * 2014-12-04 2017-12-14 The Arizona Board Of Regents On Behalf Of The University Of Arizona Systems for dissociation of biological tissues
US20190062684A1 (en) * 2012-09-29 2019-02-28 Nortis, Inc. Microfluidic System for Reproducing Functional Units of Tissues and Organs In Vitro
KR20190118702A (ko) * 2018-04-11 2019-10-21 연세대학교 산학협력단 환자 유래 종양세포를 이용한 생체외 조직체 구조물 및 이의 제조방법
CN218755788U (zh) * 2020-04-01 2023-03-28 希里斯公司 一种用于形成器官球的设备
CN116157134A (zh) * 2019-05-28 2023-05-23 希里斯公司 用于患者来源的微器官球的方法和装置

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CA2545482A1 (en) * 2003-11-10 2005-05-26 Platypus Technologies, Llc Substrates, devices, and methods for cellular assays
US7759111B2 (en) * 2004-08-27 2010-07-20 The Regents Of The University Of California Cell encapsulation microfluidic device
AU2006206426B2 (en) * 2005-01-20 2012-03-15 The Regents Of The University Of California Cellular microarrays for screening differentiation factors
KR102257305B1 (ko) * 2013-03-28 2021-05-28 더 유니버시티 오브 브리티쉬 콜롬비아 미세유동 장치 및 분비물의 다세포 검정법에서의 그의 사용 방법
EP3161052A4 (en) * 2014-06-26 2018-03-21 Northeastern University Microfluidic device and method for analysis of tumor cell microenvironments
EP3242930A4 (en) * 2015-01-07 2019-01-16 Dana-Farber Cancer Institute, Inc. MICROFLUIDIC CELL CULTURE OF TUMOR CELL SPHEROIDS FROM PATIENTS
WO2016145242A1 (en) * 2015-03-11 2016-09-15 Agenus Inc. Methods and compositions for high throughput screening of biomolecules using gel microdrops
CN110997900B (zh) * 2017-07-14 2024-06-14 多伦多大学管理委员会 用于快速产生用于化合物筛选的类器官/球状体的微流体平台
CN110004111B (zh) * 2019-04-22 2021-10-08 清华-伯克利深圳学院筹备办公室 一种类器官球体的制备方法

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001079453A1 (fr) * 2000-04-18 2001-10-25 4C Biotech Procedes de culture de cellules, amas cellulaires obtenus par ces procedes et utilisation de ceux-ci
WO2007009036A2 (en) * 2005-07-12 2007-01-18 Tissue Genesis, Inc. Apparatus and methods for preparing tissue grafts
US20190062684A1 (en) * 2012-09-29 2019-02-28 Nortis, Inc. Microfluidic System for Reproducing Functional Units of Tissues and Organs In Vitro
US20170355950A1 (en) * 2014-12-04 2017-12-14 The Arizona Board Of Regents On Behalf Of The University Of Arizona Systems for dissociation of biological tissues
KR20190118702A (ko) * 2018-04-11 2019-10-21 연세대학교 산학협력단 환자 유래 종양세포를 이용한 생체외 조직체 구조물 및 이의 제조방법
CN116157134A (zh) * 2019-05-28 2023-05-23 希里斯公司 用于患者来源的微器官球的方法和装置
CN218755788U (zh) * 2020-04-01 2023-03-28 希里斯公司 一种用于形成器官球的设备

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JP2023554171A (ja) 2023-12-26
CN218755788U (zh) 2023-03-28
KR20230143134A (ko) 2023-10-11
TW202237858A (zh) 2022-10-01
WO2022119966A1 (en) 2022-06-09

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