CN114410306A - Method for eliminating soil colletotrichum - Google Patents

Method for eliminating soil colletotrichum Download PDF

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Publication number
CN114410306A
CN114410306A CN202111588960.0A CN202111588960A CN114410306A CN 114410306 A CN114410306 A CN 114410306A CN 202111588960 A CN202111588960 A CN 202111588960A CN 114410306 A CN114410306 A CN 114410306A
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CN
China
Prior art keywords
soil
vinegar residue
biochar
ground surface
anthrax
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Pending
Application number
CN202111588960.0A
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Chinese (zh)
Inventor
刘亚柏
刘泽贤
黄洁雪
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Zhenjiang Institute of Agricultural Sciences Jiangsu Hilly Area
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Zhenjiang Institute of Agricultural Sciences Jiangsu Hilly Area
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Priority to CN202111588960.0A priority Critical patent/CN114410306A/en
Publication of CN114410306A publication Critical patent/CN114410306A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00Soil-conditioning materials or soil-stabilising materials
    • C09K17/02Soil-conditioning materials or soil-stabilising materials containing inorganic compounds only
    • C09K17/04Soil-conditioning materials or soil-stabilising materials containing inorganic compounds only applied in a physical form other than a solution or a grout, e.g. as granules or gases
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01BSOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
    • A01B79/00Methods for working soil
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00Soil-conditioning materials or soil-stabilising materials
    • C09K17/52Mulches

Abstract

The invention discloses a method for reducing soil anthrax germs, which comprises the steps of mixing vinegar residue and biochar, and uniformly spreading the mixture on the ground surface. Compared with the prior art, the method adopts the vinegar residue and the biomass charcoal to spread on the ground surface, the vinegar residue becomes a living place of the anthrax germs, and the biomass charcoal can adsorb the germs. Through practical research and comparison, the vinegar residue and the biomass charcoal are spread on the ground surface, and the abundance of the anthrax bacteria and the fusarium causing the anthrax bacteria in the soil is greatly reduced.

Description

Method for eliminating soil colletotrichum
Technical Field
The invention relates to the technical field of soil improvement of soil ecological environment, in particular to a method for reducing soil anthracnose pathogen.
Background
According to survey and statistics, the production area of Chinese fruit trees is enlarged year by year, the anthracnose pathogen has serious influence on the production of the fruit trees, especially the strawberries, grapes and the like have large loss caused by the influence of the anthracnose, and the anthracnose is a limiting factor which troubles the production of farmers all the time.
In the production process, the plant body is generally sprayed with a medicament for controlling anthracnose, the effect is poor, pathogenic bacteria easily generate resistance, and pesticide residues influence the quality safety of agricultural products. A method for eliminating anthrax bacteria in soil can eliminate or inhibit the growth of anthrax bacteria from the source of soil, block the source of harmful bacteria to plant, and is safe and effective to agricultural products.
Disclosure of Invention
The purpose of the invention is as follows: in order to overcome the defects of the prior art, the invention aims to provide the method which is safe and nontoxic to human and livestock, meets the requirements of ecological cycle agricultural production, is simple and convenient to operate, does not need large capital investment, and greatly reduces the anthracnose pathogens in soil.
The technical scheme is as follows: in order to solve the technical problems, the scheme adopted by the invention is as follows:
the application of the combination of the vinegar residue and the biochar in inhibiting the soil anthrax bacteria.
The application of the combination of the vinegar residue and the biochar in inhibiting the fusarium microorganisms in soil.
A method for eliminating anthrax bacteria in soil comprises mixing vinegar residue with charcoal, and uniformly spreading on the ground.
Preferably, the mass ratio of the vinegar residue to the biochar is (70-90): 1, more preferably 80: 1.
Preferably, the thickness of the vinegar residue and the biochar which are uniformly mixed and spread on the ground surface is 9-11cm, and more preferably 10 cm.
A method for inhibiting soil Fusarium microorganism comprises mixing vinegar residue and charcoal, and uniformly spreading on ground surface.
Preferably, the mass ratio of the vinegar residue to the biochar is (70-90): 1.
preferably, the thickness of the vinegar residue and the biochar which are uniformly mixed and spread on the ground surface is 9-11cm
The invention uses the vinegar residue and the biomass at the same time, and finds that the growth of fusarium causing the anthracnose pathogen can be inhibited while the anthracnose pathogen is reduced.
Has the advantages that: compared with the prior art, the method adopts the vinegar residue and the biomass charcoal to spread on the ground surface, the vinegar residue becomes a living place of the anthrax germs, and the biomass charcoal can adsorb the germs. Through practical research and comparison, the vinegar residue and the biomass charcoal are spread on the ground surface, and the abundance of the anthrax bacteria and the fusarium causing the anthrax bacteria in the soil is greatly reduced.
Detailed Description
The present invention will be described in further detail with reference to examples. It should be noted that modifications can be made by those skilled in the art without departing from the principle of the present invention, and these modifications should also be construed as the scope of the present invention.
The embodiment of the invention comprises the following steps:
(one), the implementation concrete example:
selecting the yellow brown soil heavy clay loam vineyard.
(II) the implementation method comprises the following steps:
a method for eliminating soil anthrax germs comprises the steps of uniformly mixing 80 parts of vinegar residue and 1 part of biomass charcoal, paving the mixture on the ground surface, and paving the mixture with the thickness of 10 cm. And taking soil half a year later to determine the microbial diversity of the soil. The method is implemented in the grapery soil in the Huayang garden of Zhenjiang agricultural science research institute in Jiangsu hilly area, adopts completely random design, and each treatment is respectively: a, pure vinegar residue; b, vinegar residue and a soil conditioner; c, vinegar residue and a compound microbial agent; d, vinegar residue and biochar; CK plowing (minor weeds); repeat 3 times.
The vinegar residue is a byproduct of Zhenjiang Hengshun vinegar plant, and the nutrient condition is as follows after fermentation and decomposition: 64.95 percent of water content, 4.67 percent of pH, 4100 cmol/kg of total salt content, 2.90 percent of P2O5 content, 0.67 percent of K2O content, 1.30 percent of N, 79.80 percent of organic matter content and 180 t/hm/m consumption2
The soil conditioner is effective Silicon (SiO) produced by agriculture science and technology limited of Zhongrui in Hebei province2) Not less than 25 percent of effective potassium (K)2O) is more than or equal to 4 percent, calcium oxide (CaO) is more than or equal to 30 percent, effective magnesium (MgO) is more than or equal to 6 percent, pH is 9.5-11.5, and the using amount is 4500 kg/hm2
The compound microbial agent is produced by Jiangsu green biotechnology limited company, the effective strains are bacillus subtilis, bacillus mucilaginosus and the like, the effective viable count is more than or equal to 200 hundred million/g, and the dosage is 15 kg/hm2
The biochar is produced by Shanxi Guangyuton science and technology Limited company, the organic carbon is more than or equal to 45 percent, the organic matter is more than or equal to 70 percent, and the N + P2O5+K2O is more than or equal to 5 percent, mineral humic acid is more than or equal to 18 percent, and the fertilizer is rich in microelements such as silicon, calcium, magnesium, zinc, molybdenum, sulfur, selenium, manganese and the like, and the dosage is 2250 kg/hm2
The implementation time is as follows: 2018, 5 and 18 months
The implementation effect is as follows: soil analysis and determination of soil layer of 0-20cm at 5 days in 12 months, and the abundances of different microbial communities in each treatment are shown in the following table:
TABLE 1 different treatment of different microbial abundance
Taxonomy A B C D CK
Fusarium (Fusarium) 8.42% 2.60% 4.21% 2.29% 5.12%
Alternaria (Alternaria) 0.36% 1.28% 0.18% 0.52% 0.40%
Saccharomyces (yeast) 0.15% 2.24% 0.10% 2.10% 0.27%
Scedosporium (branched spore) 1.18% 1.03% 3.59% 0.29% 0.08%
Gibberella (Gibberella) 1.89% 0.34% 5.68% 0.92% 0.29%
Cladosporium (Cladosporium) 0.23% 0.86% 0.13% 1.40% 0.31%
Chaetomium (Chaetomium) 2.74% 0.26% 2.71% 0.16% 0.08%
Geomyces (Saccharomyces) 0.37% 0.18% 0.32% 0.16% 0.22%
Aphanoascus (without sporangia) 1.43% 0.13% 0.72% 0.08% 0.27%
Botryotrichum (Staphylococcus) 1.09% 0.06% 4.13% 0.11% 0.07%
Dicranophora (Dictyophora fungus) 0.00% 0.00% 0.00% 0.16% 0.23%
Paraphaeosporaria (non-lymphoid coccidia) 0.72% 0.10% 0.08% 0.05% 0.05%
Podospora (retrosporean) 2.58% 0.86% 1.00% 0.06% 0.40%
Mycothermus (mold) 4.31% 0.16% 1.33% 0.10% 0.04%
Mortierella (Mortierella) 0.48% 0.58% 0.06% 0.67% 0.15%
Colletotrichum (Anthrax) 0.03% 0.28% 0.04% 0.02% 1.91%
As can be seen from Table 1, the abundances of microorganisms in different treated soils are different, and the abundances of anthracnose germs which have great influence on grapes are as follows from high to low: CK > B > C > A > D, the difference is extremely significant compared with the contrast; the anthracnose causing fusarium microorganisms have the following abundance from high to low: a > CK > C > B > D, the difference is significant. Therefore, the vinegar residue and the biochar are spread on the ground surface, so that the anthracnose and the fusarium causing the anthracnose in the soil of the plough layer can be effectively reduced, and the occurrence of grape anthracnose is inhibited.

Claims (8)

1. The application of the combination of the vinegar residue and the biochar in inhibiting the soil anthrax bacteria.
2. The application of the combination of the vinegar residue and the biochar in inhibiting the fusarium microorganisms in soil.
3. A method for eliminating anthrax bacteria in soil is characterized in that vinegar residue and charcoal are mixed and evenly spread on the ground surface.
4. The method for reducing soil anthrax bacteria of claim 3, wherein the mass ratio of the vinegar residue to the biochar is (70-90): 1.
5. the method for reducing soil anthrax bacteria of claim 3, wherein the mixture of vinegar residue and charcoal is spread on the ground to a thickness of 9-11 cm.
6. A method for inhibiting soil Fusarium microorganisms is characterized by comprising the steps of mixing vinegar residue and biochar, and uniformly spreading the mixture on the ground surface.
7. The method for inhibiting microorganisms of the genus Fusarium in soil of claim 6, wherein the mass ratio of vinegar residue to biochar is (70-90): 1.
8. the method for inhibiting Fusarium microorganisms in soil of claim 6, wherein the vinegar residue and charcoal are mixed uniformly and spread on the ground to a thickness of 9-11 cm.
CN202111588960.0A 2021-12-23 2021-12-23 Method for eliminating soil colletotrichum Pending CN114410306A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115176813A (en) * 2022-07-16 2022-10-14 杭州声能科技有限公司 Preparation method of preparation for preventing and treating anthracnose of tea trees

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002180047A (en) * 2000-12-12 2002-06-26 Biotech Japan Kk Soil-improving agent and method for producing the same
CN112592240A (en) * 2020-11-26 2021-04-02 标优美生态工程盐城有限公司 Bio-carbon-based composite conditioner for repairing soil cadmium pollution and preparation method thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002180047A (en) * 2000-12-12 2002-06-26 Biotech Japan Kk Soil-improving agent and method for producing the same
CN112592240A (en) * 2020-11-26 2021-04-02 标优美生态工程盐城有限公司 Bio-carbon-based composite conditioner for repairing soil cadmium pollution and preparation method thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
刘亚柏: "醋糟不同配比覆盖对句容旱地葡萄园土壤理化性质的影响" *
林英: "醋糟基质对土传病害的抑制效果及其拮抗微生物的研究" *
蔡昆争等: "生物炭介导植物病害抗性及作用机理" *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115176813A (en) * 2022-07-16 2022-10-14 杭州声能科技有限公司 Preparation method of preparation for preventing and treating anthracnose of tea trees

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