CN114395496A - 用于产生丙二酸的重组宿主细胞 - Google Patents

用于产生丙二酸的重组宿主细胞 Download PDF

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Publication number
CN114395496A
CN114395496A CN202111260243.5A CN202111260243A CN114395496A CN 114395496 A CN114395496 A CN 114395496A CN 202111260243 A CN202111260243 A CN 202111260243A CN 114395496 A CN114395496 A CN 114395496A
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China
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malonate
malonic acid
acid
coa
malonyl
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Pending
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CN202111260243.5A
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English (en)
Chinese (zh)
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J·A·迪特里希
J·L·福特曼
E·J·斯汀
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Lygos Inc
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Lygos Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C67/00—Preparation of carboxylic acid esters
    • C07C67/08—Preparation of carboxylic acid esters by reacting carboxylic acids or symmetrical anhydrides with the hydroxy or O-metal group of organic compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • C12Y301/02004—3-Hydroxyisobutyryl-CoA hydrolase (3.1.2.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
    • C07C51/347—Preparation of carboxylic acids or their salts, halides or anhydrides by reactions not involving formation of carboxyl groups
    • C07C51/377—Preparation of carboxylic acids or their salts, halides or anhydrides by reactions not involving formation of carboxyl groups by splitting-off hydrogen or functional groups; by hydrogenolysis of functional groups
    • C07C51/38—Preparation of carboxylic acids or their salts, halides or anhydrides by reactions not involving formation of carboxyl groups by splitting-off hydrogen or functional groups; by hydrogenolysis of functional groups by decarboxylation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C67/00—Preparation of carboxylic acid esters
    • C07C67/30—Preparation of carboxylic acid esters by modifying the acid moiety of the ester, such modification not being an introduction of an ester group
    • C07C67/31—Preparation of carboxylic acid esters by modifying the acid moiety of the ester, such modification not being an introduction of an ester group by introduction of functional groups containing oxygen only in singly bound form

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN202111260243.5A 2012-03-06 2013-03-06 用于产生丙二酸的重组宿主细胞 Pending CN114395496A (zh)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201261607479P 2012-03-06 2012-03-06
US61/607,479 2012-03-06
US201261619112P 2012-04-02 2012-04-02
US61/619,112 2012-04-02
CN201380023454.4A CN104284974B (zh) 2012-03-06 2013-03-06 用于产生丙二酸的重组宿主细胞
PCT/US2013/029441 WO2013134424A1 (en) 2012-03-06 2013-03-06 Recombinant host cells for the production of malonate

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201380023454.4A Division CN104284974B (zh) 2012-03-06 2013-03-06 用于产生丙二酸的重组宿主细胞

Publications (1)

Publication Number Publication Date
CN114395496A true CN114395496A (zh) 2022-04-26

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Family Applications (4)

Application Number Title Priority Date Filing Date
CN202111260243.5A Pending CN114395496A (zh) 2012-03-06 2013-03-06 用于产生丙二酸的重组宿主细胞
CN201710980850.6A Pending CN107586732A (zh) 2012-03-06 2013-03-06 用于产生丙二酸的重组宿主细胞
CN201380023454.4A Expired - Fee Related CN104284974B (zh) 2012-03-06 2013-03-06 用于产生丙二酸的重组宿主细胞
CN202410200340.2A Pending CN118086086A (zh) 2012-03-06 2013-03-06 用于产生丙二酸的重组宿主细胞

Family Applications After (3)

Application Number Title Priority Date Filing Date
CN201710980850.6A Pending CN107586732A (zh) 2012-03-06 2013-03-06 用于产生丙二酸的重组宿主细胞
CN201380023454.4A Expired - Fee Related CN104284974B (zh) 2012-03-06 2013-03-06 用于产生丙二酸的重组宿主细胞
CN202410200340.2A Pending CN118086086A (zh) 2012-03-06 2013-03-06 用于产生丙二酸的重组宿主细胞

Country Status (8)

Country Link
US (5) US9816114B2 (https=)
EP (2) EP3404094A1 (https=)
CN (4) CN114395496A (https=)
AU (2) AU2013230930B2 (https=)
BR (1) BR112014022117B8 (https=)
ES (1) ES2698500T3 (https=)
MY (1) MY173953A (https=)
WO (1) WO2013134424A1 (https=)

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CN114395496A (zh) 2012-03-06 2022-04-26 利戈斯股份有限公司 用于产生丙二酸的重组宿主细胞
WO2015200545A1 (en) * 2014-06-26 2015-12-30 Lygos, Inc. Recombinant host cells for the production of malonate
FR3030575A1 (fr) * 2014-12-22 2016-06-24 Inst Nat Des Sciences Appliquees De Toulouse Procede de synthese d'acides gras
WO2016130597A1 (en) * 2015-02-10 2016-08-18 GeneSys Consulting, LLC Methylmalonic acid compositions, biological methods for making same, and microorganisms for making same
US20160230198A1 (en) * 2015-02-10 2016-08-11 GeneSys Consulting, LLC Methylmalonic acid compositions, biological methods for making same, and microorganisms for making same
CN109072172A (zh) * 2015-11-12 2018-12-21 利戈斯股份有限公司 用于厌氧产生L-天冬氨酸和β-丙氨酸的重组宿主细胞和方法
KR101828551B1 (ko) * 2016-01-11 2018-02-13 한국과학기술원 말론산 생성능을 가지는 재조합 변이미생물 및 이를 이용한 말론산의 제조방법
WO2018089971A1 (en) * 2016-11-14 2018-05-17 Lygos, Inc. Process for purification of malonic acid from fermentation broth
WO2019040737A1 (en) * 2017-08-23 2019-02-28 Lygos, Inc. RECOVERING MALONIC ACID AND ITS ESTERS
CN108315343A (zh) * 2018-02-11 2018-07-24 楚雄医药高等专科学校 一种产木犀草素酵母菌株的基因合成方法及菌株和应用
WO2019160862A1 (en) * 2018-02-13 2019-08-22 Lygos, Inc. Method for preparation of diester derivatives of malonic acid
CN111936623B (zh) * 2018-03-30 2023-08-25 英威达纺织（英国）有限公司 用于生物合成制造庚二酸的材料与方法和合成多肽的利用
US20220119821A1 (en) * 2018-06-25 2022-04-21 Lygos, Inc. Recombinant host cells and methods for the production of aspartic acid and b-alanine
WO2020127506A1 (en) * 2018-12-18 2020-06-25 Alderys Malonic semi-aldehyde-producing yeasts
CN111334444A (zh) * 2018-12-19 2020-06-26 中国科学院微生物研究所 长链二元酸生产菌株及其制备方法和应用
CN110499259B (zh) * 2019-07-22 2021-07-27 浙江工业大学 一种解酯耶氏酵母yw100-1及其应用
CN112410389B (zh) * 2019-08-23 2023-07-18 中国科学院微生物研究所 支链α-酮酸脱氢酶复合体在制备丙二酸单酰辅酶A中的应用
EP4022056A4 (en) * 2019-08-30 2024-07-03 Lygos, Inc. RECOMBINANT HOST CELLS FOR THE PRODUCTION OF MALONATE
WO2021133722A1 (en) * 2019-12-23 2021-07-01 Cargill, Incorporated Fermentation pathway for producing malonic acid
US20230348353A1 (en) * 2020-05-29 2023-11-02 Cargill, Incorporated Method for recovering malonate from a fermentation broth
EP4214326A4 (en) * 2020-09-21 2024-11-06 Lygos, Inc. CONTINUOUS ION EXCHANGE AND ESTERIFICATION OF FERMENTED MALONIC ACID
WO2022250697A1 (en) * 2021-05-28 2022-12-01 Cargill, Incorporated Fermentation method for producing malonic acid
US20250320532A1 (en) * 2021-06-17 2025-10-16 Lygos, Inc. Preparing diesters of malonic acid
CN116103172B (zh) * 2021-11-11 2025-06-03 江南大学 一种酿酒酵母通过丙氨酸途径生产丙二酸的方法
CN116103173B (zh) * 2021-11-11 2025-06-03 江南大学 一种提高酿酒酵母生产丙二酸的方法
CN116135981B (zh) * 2021-11-18 2024-07-12 中国科学院天津工业生物技术研究所 一种合成丙二酸的方法及应用
CN114736841B (zh) * 2022-04-02 2023-12-19 万华化学集团股份有限公司 一种重组大肠杆菌及其制备方法与应用
WO2024054828A2 (en) * 2022-09-06 2024-03-14 Lygos, Inc. Recombinant host cells for the production of malonate
EP4585586A4 (en) * 2022-09-09 2025-12-03 Xiamen Sinopeg Biotech Co Ltd CARBON CORE CATIONIC LIPID
WO2025038447A1 (en) * 2023-08-11 2025-02-20 Ginkgo Bioworks, Inc. Engineered cells for production of malonate
CN117757766B (zh) * 2024-02-20 2024-05-14 中国科学院天津工业生物技术研究所 醛还原酶突变体及其在合成d-1,2,4-丁三醇中的应用
CN119391682A (zh) * 2025-01-03 2025-02-07 浙江科技大学 一种谷氨酸脱羧酶多位点组合突变体及其应用

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CN101052706A (zh) * 2004-07-16 2007-10-10 代尔夫特技术大学 发酵木糖的真核细胞的代谢工程
CN101283089A (zh) * 2005-10-07 2008-10-08 加利福尼亚大学董事会 修饰细胞色素p450酶的编码核酸和其应用方法
WO2011102807A1 (en) * 2010-02-19 2011-08-25 Temasek Polytechnic A substrate for immobilizing functional substances and method for preparing the same
US20110296543A1 (en) * 2006-06-01 2011-12-01 The University Of California Nucleic acids and proteins and methods for making and using them

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CN101052706A (zh) * 2004-07-16 2007-10-10 代尔夫特技术大学 发酵木糖的真核细胞的代谢工程
CN101283089A (zh) * 2005-10-07 2008-10-08 加利福尼亚大学董事会 修饰细胞色素p450酶的编码核酸和其应用方法
US20110296543A1 (en) * 2006-06-01 2011-12-01 The University Of California Nucleic acids and proteins and methods for making and using them
WO2011102807A1 (en) * 2010-02-19 2011-08-25 Temasek Polytechnic A substrate for immobilizing functional substances and method for preparing the same

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Publication number Publication date
MY173953A (en) 2020-02-28
AU2013230930B2 (en) 2018-10-11
WO2013134424A1 (en) 2013-09-12
US20160177345A1 (en) 2016-06-23
US20170362614A1 (en) 2017-12-21
CN118086086A (zh) 2024-05-28
BR112014022117B1 (pt) 2022-02-15
EP3404094A1 (en) 2018-11-21
AU2013230930A1 (en) 2014-10-23
US9816114B2 (en) 2017-11-14
CN104284974A (zh) 2015-01-14
US10472653B2 (en) 2019-11-12
EP2823032B1 (en) 2018-08-22
US20220119783A1 (en) 2022-04-21
CN104284974B (zh) 2018-06-26
EP2823032A4 (en) 2016-03-02
US20240191216A1 (en) 2024-06-13
BR112014022117B8 (pt) 2023-01-10
US20200071733A1 (en) 2020-03-05
CN107586732A (zh) 2018-01-16
AU2019200158A1 (en) 2019-01-31
US11248215B2 (en) 2022-02-15
BR112014022117A2 (https=) 2017-06-20
EP2823032A1 (en) 2015-01-14
ES2698500T3 (es) 2019-02-05

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Application publication date: 20220426