CN114395468A - Device for collecting pathogens of human respiratory diseases - Google Patents
Device for collecting pathogens of human respiratory diseases Download PDFInfo
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- CN114395468A CN114395468A CN202111598471.3A CN202111598471A CN114395468A CN 114395468 A CN114395468 A CN 114395468A CN 202111598471 A CN202111598471 A CN 202111598471A CN 114395468 A CN114395468 A CN 114395468A
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- 244000052769 pathogen Species 0.000 title claims abstract description 21
- 208000023504 respiratory system disease Diseases 0.000 title claims abstract description 13
- 239000010410 layer Substances 0.000 claims abstract description 110
- 239000000758 substrate Substances 0.000 claims abstract description 23
- 239000011241 protective layer Substances 0.000 claims abstract description 13
- 239000007787 solid Substances 0.000 claims abstract description 7
- 238000001514 detection method Methods 0.000 claims description 16
- 239000000499 gel Substances 0.000 claims description 14
- 239000000443 aerosol Substances 0.000 claims description 10
- 239000007789 gas Substances 0.000 claims description 9
- 229920001817 Agar Polymers 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- -1 polyethylene Polymers 0.000 claims description 6
- 229920003023 plastic Polymers 0.000 claims description 4
- 230000000241 respiratory effect Effects 0.000 claims description 4
- 239000004793 Polystyrene Substances 0.000 claims description 3
- 239000004417 polycarbonate Substances 0.000 claims description 3
- 229920000515 polycarbonate Polymers 0.000 claims description 3
- 229920000193 polymethacrylate Polymers 0.000 claims description 3
- 229920002223 polystyrene Polymers 0.000 claims description 3
- SCUZVMOVTVSBLE-UHFFFAOYSA-N prop-2-enenitrile;styrene Chemical compound C=CC#N.C=CC1=CC=CC=C1 SCUZVMOVTVSBLE-UHFFFAOYSA-N 0.000 claims description 3
- 229920000638 styrene acrylonitrile Polymers 0.000 claims description 3
- 239000000057 synthetic resin Substances 0.000 claims description 3
- 229920003002 synthetic resin Polymers 0.000 claims description 3
- 239000004698 Polyethylene Substances 0.000 claims description 2
- 239000004743 Polypropylene Substances 0.000 claims description 2
- 229920002472 Starch Chemical class 0.000 claims description 2
- 239000000783 alginic acid Substances 0.000 claims description 2
- 229920000615 alginic acid Polymers 0.000 claims description 2
- 229960001126 alginic acid Drugs 0.000 claims description 2
- 235000010443 alginic acid Nutrition 0.000 claims description 2
- 150000004781 alginic acids Chemical class 0.000 claims description 2
- 239000004205 dimethyl polysiloxane Substances 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims description 2
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims description 2
- 229920000573 polyethylene Polymers 0.000 claims description 2
- 229920001155 polypropylene Polymers 0.000 claims description 2
- 229920001282 polysaccharide Chemical class 0.000 claims description 2
- 239000005017 polysaccharide Chemical class 0.000 claims description 2
- 150000004804 polysaccharides Chemical class 0.000 claims description 2
- 229920000915 polyvinyl chloride Polymers 0.000 claims description 2
- 239000004800 polyvinyl chloride Substances 0.000 claims description 2
- 150000003839 salts Chemical class 0.000 claims description 2
- 239000008107 starch Chemical class 0.000 claims description 2
- 235000019698 starch Nutrition 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical class O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 239000000088 plastic resin Substances 0.000 claims 1
- 210000002345 respiratory system Anatomy 0.000 abstract description 12
- 239000001963 growth medium Substances 0.000 abstract description 5
- 238000000034 method Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 238000000605 extraction Methods 0.000 abstract description 3
- 238000004949 mass spectrometry Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 208000018569 Respiratory Tract disease Diseases 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000002795 fluorescence method Methods 0.000 description 2
- 239000000644 isotonic solution Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/0045—Devices for taking samples of body liquids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B2010/0083—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements for taking gas samples
- A61B2010/0087—Breath samples
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Hematology (AREA)
- Sustainable Development (AREA)
- Heart & Thoracic Surgery (AREA)
- Medical Informatics (AREA)
- Surgery (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a device for collecting human respiratory disease pathogens, which is of a four-layer structure, wherein the first layer is a gel layer; the second layer is a protective layer, the third layer is a transparent substrate layer, and the fourth layer is a microfluidic layer; the protective layer is used for protecting the transparent substrate layer and the microfluidic layer so as to prevent the sample collected by the microfluidic layer from being polluted in the taking-out process; the gel layer or the device needs to be frozen before use, so that the temperature difference exists between the gel layer or the device and the exhaled gas, and the exhaled gas and the droplet solution are liquefied and deposited; the transparent substrate layer and the microfluidic layer are used for collecting a sample. In addition, a layer of solid culture medium can be adhered to the surface of the face-facing side of the transparent basal layer, so that the activity of pathogens can be maintained, and the pathogens can be directly detected after extraction or detected after subsequent further culture. The device can collect human respiratory tract samples at any time without the assistance of professional persons, is convenient and quick to use, and can collect the samples all the day and detect in time.
Description
Technical Field
The invention relates to a device for collecting human respiratory tract samples, in particular to a device for collecting human respiratory tract disease pathogens, which can be used for collecting respiratory tract samples of patients suffering from respiratory tract diseases and epidemic diseases.
Background
Currently, a known respiratory tract sample collection is a pharyngeal swab collection, in which epithelial tissues around the respiratory tract, i.e., samples of mucosal surfaces, are collected by using a cotton swab or the like and placed in a culture medium for collection. Although the collection scheme is generally applied to large hospitals and respiratory diseases detection processes and has high sensitivity, sample collection needs professional medical personnel, sample treatment also needs professional medical detection mechanisms, and most importantly, sample collection cannot be performed efficiently in large batch.
Disclosure of Invention
In order to overcome the problem of collecting samples in the prior stage, the invention provides a novel device for collecting pathogens of human respiratory diseases. The mask on the market at present is only limited to isolating pathogen infection between people and cannot collect pathogens for subsequent detection, but a set of multilayer device is designed and arranged on the mask based on the cold liquefaction of gas and aerosol breathed by people, the multilayer device can collect respiratory tract samples such as liquefied aerosol, spray and the like, the collected samples are adhered to the surface of a substrate, and spectral detection, mass spectrometry detection and the like can be directly carried out. Since the device is placed on the mask, a virus sample can be collected during wear. The device can be on a large scale, and respiratory track sample is collected to convenient and fast ground, and the device is with low costs moreover, easily promotes, and it is convenient to collect the sample, and repeatability is good.
The technical scheme adopted by the invention for solving the technical problems is as follows:
an apparatus for collecting pathogens of human respiratory diseases, said apparatus having a four-layer structure, a first layer being a gel layer; the second layer is a protective layer for protecting the third layer, the third layer is a transparent substrate layer, and the fourth layer is a microfluidic layer; in the multilayer structure, the above four layers can carry out rapid heat transfer, and the first layer or the whole device needs to be frozen before use, so that the device is kept at low temperature, and the device is prevented from being overhigh in temperature along with the increase of exhaled air; the first gel layer is far away from the face of a person, the temperature is lowest, the fourth microfluidic layer is closest to a respiratory tract, the temperature of aerosol and gas in the respiratory tract is higher, when the multilayer structure is encountered, the temperature of the multilayer structure is very low due to the heat transfer effect, the gas and the aerosol can be liquefied on the surface of the third layer, and the liquefied liquid is collected in the microfluidic layer. The protective layer and the gel layer of the device are peeled off and then placed in an instrument for detection.
In the above technical solution, the gel layer further comprises salt of alginic acid, water, agar, polysaccharide or starch.
Furthermore, the protective layer is made of various films such as polyethylene, polypropylene, polyvinyl chloride and the like.
Further, the material of the transparent substrate layer may be glass, plastic (such as polymethacrylate, polystyrene, polycarbonate, styrene acrylonitrile), synthetic resin, or other transparent materials.
Furthermore, the microfluidic layer is made of resin, plastic and other materials, such as polydimethylsiloxane, polymethacrylate, polystyrene, polycarbonate and styrene acrylonitrile.
The present invention also provides another device for collecting pathogens of human respiratory diseases. In the device, other structures are not changed, and a layer of solid culture medium which can be agar can be adhered to the surface of the transparent basal layer of the third layer facing to the face. If pathogens are present in human respiratory gases and aerosols, the collected pathogens may remain active in the solid medium. The detection can be carried out directly on the substrate or can be extracted and detected after subsequent further culture. When directly detecting, optical scattering, mass spectrometry detection after ionization and the like can be used based on the third layer of the substrate; when further extraction and detection are carried out, only the solid culture medium on the third layer structure needs to be washed and separated by an isotonic solution, and PCR and fluorescence methods are used for detection after culture.
The invention has the beneficial effects that:
the device can realize the collection of human respiratory tract samples at any time without the assistance of professional persons, is convenient and quick to use, and can collect the samples in all weather and detect in time.
Drawings
The invention is further explained below with reference to the figures and examples;
FIG. 1 is a schematic diagram of the apparatus of the present invention;
FIG. 2 is a schematic view of a microfluidic layer;
FIG. 3 is a schematic view of the assembly process of the device with a mask;
wherein, gel layer 1, protective layer 2, transparent substrate layer 3, microfluid layer 4.
Detailed Description
The device for collecting pathogens of respiratory diseases of human bodies according to the invention has a four-layer structure, the first layer of the device is a frozen gel layer, the second layer is a protective layer for protecting a transparent substrate layer of the third layer, and the fourth layer is a microfluidic layer, all layers of the multilayer structure can carry out heat transfer, the first layer is far away from the face of a human body, the temperature is lowest, and the fourth layer is nearest to the respiratory tract. The temperature of the aerosol and the gas in the respiratory tract are high, when the multilayer structure is encountered, the temperature of the multilayer structure is very low due to the heat transfer effect, the gas and the aerosol can be liquefied on the surface (the third layer surface) of the multilayer structure, and the liquefied liquid is collected in the microfluidic layer.
FIG. 2 is a schematic diagram of a microfluidic layer, wherein A-A1 is the thickness of the microfluidic layer, A1-C1-D1 is a collection port (the shape is big end down), a collection groove with any shape is arranged at the lower part of the microfluidic layer, droplets are collected by the collection port, and the droplets are mainly concentrated in the region after being liquefied.
The manufacturing and using processes of the device for collecting human respiratory disease pathogens are as follows:
(1) one material of quartz glass, organic plastic film, synthetic resin or the like is used as a transparent substrate layer, and the layer is a third layer;
(2) adhering a layer of plastic as a protective layer on the upper surface of the transparent substrate layer, wherein the layer is a second layer;
(3) adhering the frozen gel to the surface of the second layer to obtain a gel layer, wherein the frozen gel layer provides low temperature for the whole device, and the layer is the first layer.
(4) The fourth layer is a microfluidic layer, and the microfluidic layer (the fourth layer) and the transparent substrate layer (the third layer) form a sample collecting device for the respiratory tract gas and the aerosol after being liquefied in the presence of cold.
(5) The assembled multilayer structure device is arranged on a mask and is arranged at the position of a breathing valve of the mask, and then the mask can be used for collecting samples of a wearer wearing the mask.
The multi-layer structure is a detachable device, which can be replaced but not reused, and the sample collection service period is 1-6 hours.
The first layer, the second layer and the third layer have the same shape and can be in various shapes such as round, square, rectangular and the like.
The collecting groove below the fourth microfluidic layer can be Y-shaped, oval, semicircular, arc-shaped and the like.
In another embodiment of the present invention, an agar layer is adhered to the surface of the transparent substrate layer (third layer) and has a thickness of 1 nm to 1 cm. If pathogens are present in human respiratory gases and aerosols, the pathogens may remain active in agar solid media. During subsequent detection, the detection can be directly carried out on the substrate or can be extracted and detected after subsequent further culture. When directly detecting, optical scattering, mass spectrometry detection after ionization and the like can be used based on the third layer of the substrate; when further extraction and detection are carried out, only the solid culture medium on the third layer structure needs to be directly washed and separated by an isotonic solution, and PCR and fluorescence methods are used for detection after culture.
Claims (10)
1. A device for collecting pathogens of human respiratory diseases is characterized in that the device has a four-layer structure, wherein the first layer is a gel layer; the second layer is a protective layer, the third layer is a transparent substrate layer, and the fourth layer is a microfluidic layer; the gel layer or the device is required to be frozen before use, so that the device is kept at a low temperature; the protective layer is used for protecting the transparent substrate layer, so that the contact surface of the protective layer and the contact surface transparent substrate layer is not polluted; the transparent substrate layer and the microfluidic layer are used for collecting a sample.
2. The device of claim 1, wherein the microfluidic layer has a collection chamber for collecting respiratory gases and aerosols that liquefy upon contact with the transparent substrate layer.
3. The device for collecting pathogens of human respiratory diseases of claim 1 wherein the surface of the transparent substrate facing the face is adhered with an agar solid medium layer.
4. The device of claim 1, wherein the gel layer comprises one or more of salts of alginic acid, water, agar, polysaccharides or starch.
5. The device of claim 1, wherein the protective layer is a polyethylene, polypropylene or polyvinyl chloride film.
6. The device of claim 1, wherein the transparent substrate layer is made of glass, plastic or synthetic resin.
7. The device of claim 1, wherein the microfluidic layer comprises polydimethylsiloxane, polymethacrylate, polystyrene, polycarbonate or styrene acrylonitrile.
8. The device for collecting pathogens of human respiratory diseases of claim 1 wherein the device is attached to a breather valve of a mask, the device being removable from the breather valve of the mask.
9. The device of claim 1, wherein the gel layer is remote from the human face.
10. The device for collecting pathogens of human respiratory diseases of claim 1 wherein the protective layer and the gel layer of the device are peeled off and placed in an instrument for detection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111598471.3A CN114395468A (en) | 2021-12-24 | 2021-12-24 | Device for collecting pathogens of human respiratory diseases |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111598471.3A CN114395468A (en) | 2021-12-24 | 2021-12-24 | Device for collecting pathogens of human respiratory diseases |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114395468A true CN114395468A (en) | 2022-04-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111598471.3A Pending CN114395468A (en) | 2021-12-24 | 2021-12-24 | Device for collecting pathogens of human respiratory diseases |
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| Country | Link |
|---|---|
| CN (1) | CN114395468A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101760428A (en) * | 2009-12-30 | 2010-06-30 | 复旦大学 | Microfluidic chip for enriching microorganisms in air and preparation method thereof |
| CN111227347A (en) * | 2020-02-23 | 2020-06-05 | 刘巍 | Special enrichment sampling medical mask for respiratory tract transmitted pathogens |
| CN111513377A (en) * | 2020-03-05 | 2020-08-11 | 广州市宝创生物技术有限公司 | Novel mask for collecting coronavirus pathogens in aerosol and droplet and collection method |
| CN111528928A (en) * | 2020-07-13 | 2020-08-14 | 深圳华大基因股份有限公司 | Sampling mask |
| CN212629960U (en) * | 2020-05-20 | 2021-03-02 | 汪梓墨 | Mask cooling paste |
| CN214654606U (en) * | 2020-09-15 | 2021-11-09 | 埃肯有机硅（上海）有限公司 | Adhesive tape with multilayer structure and mask using same |
-
2021
- 2021-12-24 CN CN202111598471.3A patent/CN114395468A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101760428A (en) * | 2009-12-30 | 2010-06-30 | 复旦大学 | Microfluidic chip for enriching microorganisms in air and preparation method thereof |
| CN111227347A (en) * | 2020-02-23 | 2020-06-05 | 刘巍 | Special enrichment sampling medical mask for respiratory tract transmitted pathogens |
| CN111513377A (en) * | 2020-03-05 | 2020-08-11 | 广州市宝创生物技术有限公司 | Novel mask for collecting coronavirus pathogens in aerosol and droplet and collection method |
| CN212629960U (en) * | 2020-05-20 | 2021-03-02 | 汪梓墨 | Mask cooling paste |
| CN111528928A (en) * | 2020-07-13 | 2020-08-14 | 深圳华大基因股份有限公司 | Sampling mask |
| CN214654606U (en) * | 2020-09-15 | 2021-11-09 | 埃肯有机硅（上海）有限公司 | Adhesive tape with multilayer structure and mask using same |
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